Biochemical Engineering Journal最新文献_第7页

Enhancing gibberellic acid production in Fusarium fujikuroi through regulatory factors optimization and overexpression of key genes 通过调控因子优化和关键基因过表达提高藤黑镰刀菌赤霉素产量

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-11-11 DOI: 10.1016/j.bej.2025.110004

Yuanshan Wang , Lang Wang , Liangzhuang Tan , Yihang Wu , Chunyue Weng , Yuke Cen , Zhiqiang Liu , Yuguo Zheng

Gibberellic acid (GA₃) is a crucial phytohormone involved in plant growth regulation with widespread applications in agriculture and other fields. In this study, GA₃ synthesis in an industrial GA₃ producer F. fujikuroi strain FF00 was improved with a strategy by reprogramming the regulatory network and overexpressing key genes. Three positive regulators AreA (the nitrogen regulator), Lae1 (the velvet complex component), and Hat1 (the histone modification regulator) were identified by screening regulators affecting GA₃ biosynthesis. Mutant FF18–4 with GA₃ titer of 2.61 g/L in shake-flask fermentation was constructed by co-overexpressing AreA, Lae1, and Hat1 in strain FF00. Comparative transcriptomic analysis revealed that reprogramming of the regulatory network resulted in the down-regulation of two key genes (Ggs2 and Cps/Ks) in the GA₃ biosynthetic pathway which reduced the supply of GA₃ precursor geranylgeranyl pyrophosphate. Ggs2 and Cps/Ks genes were further co-overexpressed to enhance metabolic flux for GA₃ biosynthesis. The GA₃ titer of the resulting mutant FF19–5 reached 2.73 g/L, which was 49.2 % higher than that of strain FF00. Therefore, the GA₃ biosynthesis of strain FF00 was significantly improved by metabolic network reprogramming and metabolic balance. The established strategy provided the basis for GA₃ over-producer construction and may be helpful for the synthesis of other chemicals with microbial cell factories.

赤霉素（giberellic acid, GA3）是一种重要的植物激素，在农业和其他领域有着广泛的应用。本研究通过对GA3工业产生菌F. fujikuroi菌株FF00的调控网络进行重编程和过表达关键基因的策略，提高了GA3的合成。通过筛选影响GA3生物合成的调节因子，鉴定出3个正调节因子AreA（氮调节因子）、Lae1（丝绒复合物组分）和Hat1（组蛋白修饰调节因子）。在菌株FF00中共过表达AreA、Lae1和Hat1，构建摇瓶发酵GA3滴度为2.61 g/L的突变体FF18-4。比较转录组学分析显示，调控网络的重编程导致GA3生物合成途径中两个关键基因Ggs2和Cps/Ks的下调，从而减少了GA3前体香叶酰焦磷酸的供应。Ggs2和Cps/Ks基因进一步共过表达，以增强GA3生物合成的代谢通量。突变体FF19-5的GA3滴度达到2.73 g/L，比菌株FF00提高49.2% %。因此，通过代谢网络重编程和代谢平衡，菌株FF00的GA3生物合成得到了显著提高。所建立的策略为GA3过度生产者的构建提供了基础，并可能对微生物细胞工厂合成其他化学品有所帮助。

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引用次数: 0

In-situ synthesis of regenerable ZIF-67/cellulose aerogel composites for rapid and high-capacity tetracycline adsorption 原位合成可再生ZIF-67/纤维素气凝胶复合材料快速、高容量吸附四环素

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-11-10 DOI: 10.1016/j.bej.2025.110003

Meijing Liu , Shiye Cai , C. Srinivasakannan , Gang Xue , Li Wang , Yaping Wang , Xinhui Duan

The presence of tetracycline (TC) in aquatic environments poses significant risks to ecosystems and human health. Although metal-organic frameworks (MOFs) show promise for TC removal, their powdery nature limits their application in water treatment. This study developed a hierarchical porous ZIF-67/cellulose aerogel composite (ZIF-67/CA) through crosslinking and in-situ growth. The composite exhibits a high specific surface area of 346 m²/g and large pore volume of 1.13 cm³/g, with its hierarchical pore architecture contributing to a remarkable TC adsorption capacity of 961.54 mg/g and rapid kinetics following the pseudo-second-order model. The adsorbent maintained over 61 % of its initial capacity after five regeneration cycles via H₂O₂-induced degradation. Combined with comprehensive mechanistic analysis, these findings demonstrate ZIF-67/CA as an efficient and reusable solution for antibiotic-contaminated water treatment.

Synopsis

The ZIF-67/CA composite synthesized via in-situ growth demonstrates efficient adsorption of TC through multiple synergistic adsorption mechanisms.

四环素（TC）在水生环境中的存在对生态系统和人类健康构成重大风险。虽然金属有机框架（mof）显示出去除TC的希望，但它们的粉状性质限制了它们在水处理中的应用。本研究通过交联和原位生长制备了一种分级多孔ZIF-67/纤维素气凝胶复合材料（ZIF-67/CA）。该复合材料具有346 m2/g的高比表面积和1.13 cm3/g的大孔容，其层次化的孔隙结构使其吸附TC的容量达到了961.54 mg/g，并且具有快速的准二阶动力学模型。经过5次h2o2诱导降解再生后，吸附剂的容量保持在初始容量的61% %以上。结合综合机理分析，这些发现表明ZIF-67/CA是一种高效且可重复使用的抗生素污染水处理解决方案。摘要原位生长法制备的ZIF-67/CA复合材料通过多种协同吸附机制对TC进行了高效吸附。

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引用次数: 0

Multi-substrate kinetic framework and wool-immobilised Pseudomonas fluorescens lipase (WPFL) for solvent-free biocatalysis of terpene esters from rose geranium oil 多底物动力学框架和羊毛固定化荧光假单胞菌脂肪酶（WPFL）用于无溶剂催化玫瑰天竺葵油中萜烯酯的研究

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-11-10 DOI: 10.1016/j.bej.2025.110002

Alisa S. Wikaputri , Azimmatul Ihwah , Derek J. Irvine , Robert A. Stockman , Stephen Grebby , Parimala Shivaprasad

We present a green, solvent-free enzymatic strategy for the valorisation of rose geranium essential oil into bio-based terpene esters using wool-immobilized Pseudomonas fluorescens lipase (WPFL). Among 30 volatiles, the major constituents citronellol and geraniol were selectively transformed. The process achieved complete conversion of geraniol and 88 % conversion of citronellol within 5 h, doubling productivity relative to conventional solvent-based methods while maintaining efficiency across varied substrate concentrations. Wool, as a renewable and biodegradable support, ensured uniform enzyme distribution and high catalytic stability. Kinetic analysis using specificity constants (kcat/K_M) provided turnover numbers of 5.03 s⁻¹ (geraniol) and 0.25 s⁻¹ (citronellol), establishing the first multi-substrate kinetic framework for essential oil transesterification. Compared to commercial catalysts like Novozym 435, WPFL offers a scalable, cost-effective, and environmentally benign route to sustainable fragrance and flavour esters. Sensory analysis before and after transesterification revealed altered olfactory profiles, highlighting the potential to generate novel fragrance profiles through biocatalysis. This work demonstrates how solvent-free processing, renewable enzyme supports, and kinetic modelling can be integrated to enable efficient, scalable production of bio-based chemicals.

我们提出了一种绿色、无溶剂的酶促策略，利用羊毛固定化荧光假单胞菌脂肪酶（WPFL）将玫瑰天竺葵精油转化为生物基萜烯酯。30种挥发物中，主要成分香茅醇和香叶醇被选择性转化。该工艺在5 h内实现了香叶醇的完全转化和香薰醇的88 %的转化率，相对于传统的溶剂基方法的生产率翻了一番，同时在不同的底物浓度下保持效率。羊毛作为可再生和可生物降解的载体，保证了酶的均匀分布和高催化稳定性。使用特异性常数（kcat/KM）的动力学分析提供了5.03 s⁻¹ （香叶醇）和0.25 s⁻¹ （香橼醇）的周转数，建立了第一个精油酯交换的多底物动力学框架。与Novozym 435等商业催化剂相比，WPFL提供了一种可扩展、经济高效、环保的可持续香料和风味酯的途径。酯交换反应前后的感官分析揭示了嗅觉特征的改变，强调了通过生物催化产生新型香味特征的潜力。这项工作展示了如何将无溶剂加工、可再生酶支持和动力学建模集成在一起，以实现高效、可扩展的生物基化学品生产。

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引用次数: 0

Process-based strategies improve xylitol production by recombinant S. cerevisiae from sugarcane straw hydrolysate 基于工艺的策略提高了重组酿酒酵母从甘蔗秸秆水解物中生产木糖醇的能力

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-11-10 DOI: 10.1016/j.bej.2025.109999

Thiago I. Gaspar, Frank U.S. Lizarazo, Gonçalo A.G. Pereira, Fellipe S.B. de Mello

Xylitol, a naturally occurring biomolecule, is utilized in various commercial applications, with its market expected to reach USD 1.7 billion by 2028. Large-scale commercial production typically involves rigorous chemical processes, making xylitol biosynthesis an attractive alternative. The generally recognized as safe (GRAS) yeast Saccharomyces cerevisiae emerges as an appealing host for this purpose due to its robustness and efficiency under industrial conditions. However, research on utilizing this yeast for xylitol production from biomass hydrolysates remains sparse. This study investigates bioprocessing improvements to enhance xylitol production from sugarcane straw hydrolysate using recombinant S. cerevisiae strains FMYX and CENPKX, derived from industrial and laboratory backgrounds. Strategies such as increased cell inoculum and nutrient supplementation were employed to optimize xylose conversion in batch processes. Adjusting the pitching rate enhanced xylitol productivity by up to 1.32-fold, while yeast extract and peptone (YP) supplementation resulted in a 1.42-fold increase in xylitol production by FMYX. Notably, under very low cell inoculum conditions, YP supplementation led to an 8.7-fold increase in xylitol production by FMYX, reaching levels comparable to those obtained with a 25-fold higher inoculum in the absence of supplementation. This research provides an efficient alternative process for xylitol production, leveraging existing industrial infrastructure. While techno-economic analysis remains imperative, results represent novel approaches towards sustainable xylitol biosynthesis using modified S. cerevisiae.

木糖醇是一种天然存在的生物分子，被用于各种商业应用，到2028年，其市场预计将达到17亿美元。大规模商业生产通常涉及严格的化学过程，使木糖醇生物合成成为一种有吸引力的替代品。公认的安全（GRAS）酵母菌（Saccharomyces cerevisiae）因其在工业条件下的稳健性和效率而成为一种有吸引力的宿主。然而，利用这种酵母从生物质水解物中生产木糖醇的研究仍然很少。本研究利用具有工业和实验室背景的重组酿酒酵母菌株FMYX和CENPKX，研究了对甘蔗秸秆水解产物进行生物加工以提高木糖醇产量的方法。采用增加细胞接种量和添加营养物质等方法优化木糖的批量转化。调整俯仰率可使木糖醇产量提高1.32倍，而酵母提取物和蛋白胨（YP）的添加使FMYX的木糖醇产量提高1.42倍。值得注意的是，在非常低的细胞接种量条件下，添加YP导致FMYX木糖醇产量增加8.7倍，达到与未添加YP时接种量增加25倍的水平相当。本研究为木糖醇生产提供了一种有效的替代工艺，利用现有的工业基础设施。虽然技术经济分析仍然是必要的，但结果代表了利用改性酿酒酵母可持续木糖醇生物合成的新方法。

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引用次数: 0

Operational performance and mechanistic analysis of the anaerobic digestion of iron-rich waste activated sludge 富铁废物活性污泥厌氧消化的运行性能及机理分析

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-11-09 DOI: 10.1016/j.bej.2025.110000

Longmei Xu , Yuqi Wu , Xiulan Song

The effects of high iron content on the anaerobic digestion of waste activated sludge were investigated in this study. Biochemical methane potential tests indicated that waste activated sludge with high iron content (54.7 mg/g total solids) significantly reduced cumulative methane production and methane content compared to waste activated sludge with low iron content (25.98 mg/g total solids). Furthermore, the addition of magnetite led to negligible enhancement of methane production. Physicochemical analyses indicated that pH, oxidation–reduction potential and alkalinity were maintained within normal ranges during the anaerobic digestion of iron-rich waste activated sludge, whereas the solubilisation rate of particulate organic matter was notably reduced due to the physical binding of iron ions, iron oxides and organic particles. Microbial community analysis indicated that dissimilatory iron-reducing bacteria (Longilinea) were enriched under high-iron conditions, competing with methanogens for electrons and thereby inhibiting methane production. Based on microbial community structure and metabolic function analyses, acetotrophic methanogenesis and hydrogenotrophic methanogenesis were identified as the dominant metabolic pathways for methane production. Overall, anaerobic digestion alone may not be effective for the treatment of iron-rich waste activated sludge due to low energy recovery and high operating costs. This study provides new insights into the mechanisms underlying the poor anaerobic digestion performance of iron-rich waste activated sludge and offers practical guidance to improve methane recovery and sludge disposal.

研究了高铁含量对废活性污泥厌氧消化的影响。生化甲烷电位试验表明，高铁含量（54.7 mg/g固体总量）的废活性污泥与低铁含量（25.98 mg/g固体总量）的废活性污泥相比，累积甲烷产量和甲烷含量显著降低。此外，磁铁矿的加入对甲烷产量的提高作用微不足道。理化分析表明，富铁废物活性污泥厌氧消化过程中pH值、氧化还原电位和碱度保持在正常范围内，而颗粒有机物的溶解速率由于铁离子、氧化铁和有机颗粒的物理结合而明显降低。微生物群落分析表明，在高铁条件下，异化铁还原菌（Longilinea）富集，与产甲烷菌竞争电子，从而抑制甲烷的产生。通过微生物群落结构和代谢功能分析，确定了乙营养化产甲烷和氢营养化产甲烷是甲烷产生的主要代谢途径。总的来说，由于能量回收率低和运行成本高，单靠厌氧消化可能不能有效地处理富铁废物活性污泥。本研究为富铁废物活性污泥厌氧消化性能差的机理提供了新的见解，并为提高甲烷回收和污泥处理提供了实践指导。

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引用次数: 0

Performance and mechanisms of an algae-bacteria symbiotic system for treating low carbon-to-nitrogen ratio wastewater 藻类-细菌共生系统处理低碳氮比废水的性能和机制

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-11-08 DOI: 10.1016/j.bej.2025.109995

Lin Zhao , Yuwei Xu , Yifan Zhang , Yuhan Huang , Guanqin Huang , Zhangli Hu , Chuansheng Wu , Jun Tang

The integration of microalgae and bacteria offers a promising approach for nutrient recovery and resource valorization in wastewater treatment, particularly under carbon-limited conditions. This study investigates the performance and potential mechanisms of a Tetradesmus obliquus-based algae–bacteria symbiotic (ABS) system for treating low carbon-to-nitrogen (C/N) ratio wastewater under varying light conditions, initial inoculation densities, and aeration rates. Optimal operating conditions were identified as an initial OD₆₈₀ of 0.2, aeration rate of 1 L air/min, light intensity of 112 μmol/(m²·s), and a 16 L:8D photoperiod. Under these conditions, the ABS system achieved near-complete removal of total nitrogen (95.85 %) and total phosphorus (97.96 %). Concurrently, biomass dry weight increased from 0.05 to 0.37 g/L, with the system producing substantial amounts of protein, polysaccharides, and lipids, demonstrating strong potential for bioresource recovery. Metagenomic and transcriptomic analyses revealed a clear microbial succession from Chlorophyta to Pseudomonadota and Aquibium dominance, coupled with upregulation of nitrogen (narG, nirK, NRT2, glnA) and phosphorus (ppk1, pstA, phoB) metabolism genes, indicating enhanced nutrient assimilation under low C/N stress. Overall, the ABS system effectively coupled wastewater treatment with biosynthetic productivity. These findings highlight its potential as a sustainable platform for integrated nutrient removal and high-value biomass generation in low C/N ratio wastewater scenarios.

微藻和细菌的结合为污水处理中的养分回收和资源增值提供了一种很有前途的方法，特别是在碳限制条件下。本研究探讨了斜四角藻-细菌共生（ABS）系统在不同光照条件、初始接种密度和曝气速率下处理低碳氮比废水的性能和潜在机制。最佳操作条件为初始OD680为0.2，曝气速率为1 L air/min，光照强度为112 μmol/(m²·s)，光周期为16 L:8D。在此条件下，ABS系统几乎完全去除了总氮（95.85 %）和总磷（97.96 %）。同时，生物质干重从0.05提高到0.37 g/L，系统产生大量的蛋白质、多糖和脂质，显示出很强的生物资源回收潜力。元基因组学和转录组学分析显示，从绿藻到假单胞藻具有明显的微生物演替和水藻优势，同时氮（narG、nirK、NRT2、glnA）和磷（ppk1、pstA、phoB）代谢基因上调，表明低碳氮胁迫下营养同化增强。总体而言，ABS系统有效地将废水处理与生物合成生产力结合起来。这些发现突出了它作为一个可持续平台的潜力，可以在低碳氮比的废水场景中综合去除营养物和产生高价值的生物质。

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引用次数: 0

Insights into iron-doped keratin derived via alkali hydrolysis for nitrate adsorption: Experimental and computational modeling 洞察铁掺杂角蛋白衍生通过碱水解硝酸盐吸附：实验和计算模型

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-11-08 DOI: 10.1016/j.bej.2025.109997

Riya Sen , Kamalesh Sen , Naba Kumar Mondal

This study explores the sustainable and efficient approach to nitrate remediation using iron-doped keratin (Fe@KHH) synthesized by impregnation of iron in keratin (KHH) extracted from human hair waste by alkaline hydrolysis. By iron incorporation porosity, functional group and surface charge were enhanced by which adsorption capacity was increased from 33.3 mg/g to 81.69 mg/g. Response Surface Methodology using a Box–Behnken design identified pH and contact time are the most influential factors, with the model predicting 94.13 % nitrate removal under optimal conditions and a high desirability score (1.000). Kinetic studies indicated pseudo-second-order behavior for both adsorbents, while thermodynamic analysis confirmed the exothermic and spontaneous nature of the process. Competitive ion studies and desorption tests demonstrated Fe@KHH’s superior selectivity and reusability, retaining 65 % capacity after six cycles. Density Functional Theory, Density of States, and Molecular Dynamicssimulations supported experimental findings by revealing strong nitrate interaction, reduced HOMO–LUMO gap, and favorable non-covalent stabilization. Collectively, these results highlight Fe@KHH as a highly effective, low-cost, and eco-friendly adsorbent for nitrate removal in water treatment systems.

本研究利用人发废弃物中提取的角蛋白（KHH），通过碱性水解将铁浸渍合成铁掺杂角蛋白（Fe@KHH），探索可持续、高效的硝酸盐修复方法。铁的掺入增强了孔隙度、官能团和表面电荷，使吸附容量从33.3 mg/g提高到81.69 mg/g。采用Box-Behnken设计的响应面法确定pH值和接触时间是影响最大的因素，该模型预测在最佳条件下硝酸盐去除率为94.13% %，期望分数为1.000。动力学研究表明两种吸附剂的伪二阶行为，而热力学分析证实了该过程的放热和自发性质。竞争离子研究和解吸试验表明Fe@KHH具有优异的选择性和可重复使用性，在六个循环后保持65 %的容量。密度泛函数理论、态密度和分子动力学模拟通过揭示强硝酸盐相互作用、减小HOMO-LUMO间隙和有利的非共价稳定来支持实验结果。总的来说，这些结果突出Fe@KHH作为水处理系统中硝酸盐去除的高效，低成本和环保吸附剂。

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引用次数: 0

Hybrid model development to support process understanding: An industrial mRNA in vitro transcription case study 混合模型开发以支持过程理解：一个工业mRNA体外转录案例研究

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-11-07 DOI: 10.1016/j.bej.2025.109998

Guilherme Ramos , Alice Rosa , Konstantinos Alexias , Hubert Boulic , Sofia Moreira , Moritz von Stosch

Optimizing the in vitro transcription (IVT) process for mRNA production of large mRNA molecules is a challenge. In this study, a hybrid model was developed to aid in understanding the process behavior for a specific mRNA sequence. The hybrid model strategy allowed using the available fundamental knowledge while accounting for changes in the process that would be difficult to incorporate into a more mechanistic approach, e.g., lot-to-lot variations in starting materials. The model showed excellent performance on a representative test set, capturing the evolution of total mRNA concentration, intact/fragmented mRNA, NTPs and pH (not controlled in the current setting) for multiple process parameter configurations. Further insights were extracted via what-if simulations and Shapley value analysis, which are presented in a qualitative manner. The results confirm the importance of the ratio of Magnesium to NTP molecules on the final process yield (a ∼10 % variation in Magnesium/NTP can change transcription rate by ∼30 %), highlighting also the influence of temperature (a 5°C can change rates of transcription and fragmentation by 150 % and 175 %, respectively), the DNA material lot and mix preparation time on process outcome. The model structure is such that additional inputs could be added in subsequent activities for scale-up support or the development of processes for other mRNA molecules.

优化体外转录（IVT）过程以生产大mRNA分子是一个挑战。在这项研究中，开发了一个杂交模型来帮助理解特定mRNA序列的过程行为。混合模型策略允许使用可用的基础知识，同时考虑到过程中的变化，这些变化很难合并到更机械的方法中，例如，起始材料的批对批变化。该模型在具有代表性的测试集上表现出色，捕获了多个工艺参数配置下总mRNA浓度、完整/碎片化mRNA、ntp和pH（当前设置不受控制）的演变。进一步的见解是通过假设模拟和沙普利值分析，这是在定性的方式提出。结果证实了镁与NTP分子的比例对最终工艺收率的重要性（镁/NTP变化约10 %可使转录率改变约30 %），同时也强调了温度（5°C可使转录率和片段率分别改变150 %和175 %）、DNA材料数量和混合制备时间对工艺结果的影响。模型结构是这样的，可以在后续活动中添加额外的输入，以扩大支持或开发其他mRNA分子的过程。

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引用次数: 0

Use of free nitrous acid from partial nitrification reactor for the sanitization of digester effluents and Class A biosolids production 部分硝化反应器游离亚硝酸盐用于消化废水的卫生处理及甲类生物固体的生产

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-11-07 DOI: 10.1016/j.bej.2025.109993

Zeinab Morhell , Gabriel Martínez , Oscar Franchi , Dafne Crutchik , Cristopher Da Silva , Lorna Guerrero , Alba Pedrouso , Ángeles Val del Río , Anuska Mosquera-Corral , José Luis Campos

Mesophilic anaerobic digestion alone typically fails to meet the pathogen reduction criteria required for Class A biosolids (<1000 MPN g⁻¹ TS of fecal coliforms), limiting their agricultural reuse. Since many wastewater treatment plants (WWTPs) have implemented partial nitrification/anammox (PN/A) processes in their sludge lines, this study proposes using free nitrous acid (FNA), generated in situ within a partial nitrification (PN) reactor, as a cost-effective biocidal agent to achieve Class A biosolids. A PN reactor was directly fed with the effluent from an anaerobic digester treating thermally hydrolyzed sludge and operated at hydraulic retention times (HRTs) between 1.5 and 4 days. The reactor achieved ammonium oxidation efficiencies of 46–49 %, generating FNA concentrations of 0.95–2.40 mg HNO₂ L⁻¹ that simultaneously promoted pathogen inactivation within the reactor, resulting in total coliform removals of 77–89 % and meeting Class A standards. Batch kinetic assays simulating mild acidification (pH 5–6) showed that > 99 % coliform removal could be achieved within 2 h, confirming the feasibility of a compact post-treatment step if needed. Economic evaluation indicated that the higher aeration energy demand was compensated by lower sludge management costs and potential revenue from Class A biosolids. Overall, the proposed configuration integrates nitrogen removal and sludge sanitization, providing a sustainable, energy-efficient, and economically viable strategy for biosolids reuse in WWTPs.

仅嗜中温厌氧消化通常不能满足A类生物固体（1000 MPN g - 1 TS粪便大肠菌）所需的病原体减少标准，限制了它们的农业重复利用。由于许多污水处理厂（WWTPs）已经在其污泥生产线上实施了部分硝化/厌氧氨氧化（PN/A）工艺，因此本研究建议使用在部分硝化（PN）反应器内原位生成的游离亚硝酸盐（FNA）作为一种具有成本效益的杀菌剂来获得A类生物固体。将处理热水解污泥的厌氧沼气池出水直接送入PN反应器，并在水力停留时间（hrt）为1.5 ~ 4天的条件下运行。反应器的氨氧化效率为46-49 %，产生的FNA浓度为0.95-2.40 mg HNO₂L⁻¹ ，同时促进了反应器内病原体的灭活，使总大肠菌群去除率达到77-89 %，达到A类标准。模拟温和酸化（pH 5-6）的批量动力学分析表明，在2 h内可以实现>； 99 %大肠菌群的去除，确认了如果需要的话，紧凑的后处理步骤的可行性。经济评价表明，较高的曝气能量需求被较低的污泥管理成本和A类生物固体的潜在收入所补偿。总体而言，所提出的配置集成了氮去除和污泥消毒，为污水处理厂的生物固体再利用提供了可持续、节能和经济可行的策略。

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引用次数: 0

Staged anaerobic strategy enhances endogenous partial denitrifying phosphorus removal: Performance improvement and microbial mechanisms 阶段厌氧策略增强内源性部分反硝化除磷：性能改进和微生物机制

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-11-07 DOI: 10.1016/j.bej.2025.109992

Dong Li , Songwei Yang , Yuhang Zhou , Shuai Li , Fei Ding , Huiping Zeng , Jie Zhang

The endogenous partial denitrifying phosphorus removal (EPDPR) process, driven by denitrifying polyphosphate-accumulating organisms (DPAOs), can provide a source of NO₂⁻−N for anaerobic ammonium oxidation (annmmox). However, unstable phosphorus removal and insufficient NO₂⁻ −N accumulation remain challenges. Previous studies have enhanced DPAOs activity by optimizing carbon sources and C/N ratios, the effects of staged anaerobic configuration on the EPDPR process remain unclear. In this study, one-stage, two-stage, and three-stage anaerobic modes were implemented in a sequencing batch reactor to systematically evaluate the effects of staged anaerobic strategy on system performance and microbial community structure. Results showed that, under identical carbon conditions, the maximum phosphorus release rate of DPAOs increased from 12.35 to 17.24 mg P/(g VSS·h), and their contribution to endogenous carbon storage rose from 28.10 % to 43.19 %. Under the three-stage anaerobic condition, total phosphorus removal efficiency and nitrite accumulation rate reached 94.58 % and 74.01 %, respectively. The results of High-throughput sequencing revealed that the relative abundances of dominant DPAOs genera represented by Candidatus_Accumulibacter, Pseudomonas, and Thauera increased to 2.43 %, 5.85 %, and 5.24 %, respective. In comparison Nitrospira abundances decreased to 0.20 %, suppressing further NO₂⁻−N reduction and providing favorable conditions for anammox process coupling.

由反硝化聚磷生物（DPAOs）驱动的内源性部分反硝化除磷（EPDPR）过程可以为厌氧氨氧化（annmmox）提供NO2−−N的来源。然而，磷去除不稳定和NO2−−N积累不足仍然是挑战。已有研究通过优化碳源和碳氮比来提高DPAOs的活性，但阶段厌氧配置对EPDPR过程的影响尚不清楚。本研究在序批式反应器中实施了一级、二级和三级厌氧模式，系统评估了分级厌氧策略对系统性能和微生物群落结构的影响。结果表明，在相同碳量条件下，DPAOs的最大磷释放速率从12.35增加到17.24 mg P/(g VSS·h)，对内源碳储量的贡献从28.10 %增加到43.19 %。在三级厌氧条件下，总磷去除率和亚硝酸盐积累率分别达到94.58 %和74.01 %。高通量测序结果显示，以Candidatus_Accumulibacter、Pseudomonas和Thauera为代表的优势DPAOs属的相对丰度分别增加到2.43 %、5.85 %和5.24 %。相比之下，硝化螺旋菌丰度降至0.20 %，抑制了NO2−−N的进一步还原，为厌氧氨氧化过程耦合提供了有利条件。

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