Biochemical Engineering Journal最新文献_第7页

Model-based optimization of stripping onset in Saccharomyces cerevisiae very-high-gravity ethanol fermentations 基于模型的酿酒酵母极重乙醇发酵脱皮起始优化

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-03-01 Epub Date: 2025-12-27 DOI: 10.1016/j.bej.2025.110057

I.I.K. Veloso , V.T. Mazziero , D.A. Lemos , A.J.G. Cruz , M.O. Cerri , A.C. Badino

Very-high-gravity (VHG) fermentation increases ethanol yield and decreases water use, but it imposes severe osmotic and ethanolic stress on Saccharomyces cerevisiae, which frequently limits overall productivity. In situ ethanol removal through CO₂ gas stripping can mitigate this inhibition. However, the optimal timing for stripping onset under different temperature conditions remains not fully characterized. This study examined the effect of stripping initiation timing in VHG fed-batch fermentations at 28, 30, 32, and 34°C. A mechanistic fermentation model based on Monod-type kinetics with ethanol inhibition terms and coupled gas-liquid mass transfer was estimated using differential evolution. Distinct, temperature-specific ethanol concentration thresholds for stripping onset were identified, which maximized ethanol productivity (

P_{CE}

). Plateau analysis, based on identification of concentration regions where delayed initiation produced negligible changes in

P_{CE}

, refined these thresholds to within ±0.5 % of the maximum productivity. This resulted in CO₂ gas savings equivalent to 1.05 ± 0.06 h per fermentation cycle. Model-based time-varying temperature control optimization predicted an increase in ethanol productivity to 12.32 g L⁻¹ h⁻¹. The findings provide a simulation and parameter estimation framework for temperature-integrated extractive control strategies to improve ethanol production in high-biomass VHG fermentations.

非常高重力（VHG）发酵提高了乙醇产量，减少了水的使用，但它对酿酒酵母施加了严重的渗透和乙醇压力，这往往限制了总体生产力。通过CO2气提原位去除乙醇可以减轻这种抑制作用。然而，在不同温度条件下，汽提开始的最佳时机尚未完全确定。本研究考察了在28、30、32和34℃条件下VHG补料分批发酵中剥离起始时间的影响。利用微分演化方法估计了一个基于monod型动力学、乙醇抑制项和气液耦合传质的机制发酵模型。确定了不同的温度特异性乙醇浓度阈值，以最大化乙醇生产率（PCE）。平台分析，基于识别延迟起始产生可忽略不计的PCE变化的浓度区域，将这些阈值细化到最大生产力的±0.5%以内。这导致二氧化碳气体节省相当于1.05±0.06小时每个发酵周期。基于模型的时变温度控制优化预测乙醇产量将增加到12.32 g L - 1 h - 1。研究结果为温度集成萃取控制策略提供了模拟和参数估计框架，以提高高生物量VHG发酵的乙醇产量。

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引用次数: 0

DLF-BBP: A novel deep learning framework for blood-brain barrier penetrating peptides design DLF-BBP：一种新的血脑屏障穿透肽设计深度学习框架

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-03-01 Epub Date: 2025-11-22 DOI: 10.1016/j.bej.2025.110014

Lei Huang , Zhanglong Li , Zhengtai Li , Hongjiang Li , Tiejun Bing , Yingji Li , Changyuan Yu

The blood-brain barrier (BBB) maintains brain homeostasis by selectively regulating substance exchange but also limits central nervous system (CNS) drug delivery. Blood-brain barrier penetrating peptides (BBPs) are short peptides capable of traversing the BBB, serving as drug carriers or therapeutic agents. Therefore, designing novel BBPs is crucial for overcoming the BBB's limitations and advancing CNS-targeted drug development. In this study, we propose a novel deep learning framework for BBPs design (DLF-BBP). Beginning with a Self-Attention Generative Adversarial Network (SAGAN) model to generate novel candidate BBPs that were evaluated in silico, we subsequently constructed a predictive model based on a Convolutional Neural Network (CNN) for the initial screening of GAN-designed BBPs, achieving an area under the curve (AUC) of 0.988 and an accuracy of 0.954. Further multi-step screening was conducted using physicochemical property analysis, molecular docking, and online platforms. Finally, the filtered candidate GAN-designed BBPs were validated through in vitro BBB penetration assay, leading to the identification of three promising GAN-designed BBPs with high permeation capability, demonstrating penetration comparable to that of the positive control Lixisenatide. Our research offers new possibilities for treating neurological disorders.

血脑屏障（BBB）通过选择性调节物质交换来维持大脑稳态，但也限制了中枢神经系统（CNS）的药物传递。血脑屏障穿透肽（BBPs）是一种能够穿过血脑屏障的短肽，可作为药物载体或治疗剂。因此，设计新的血脑屏障对于克服血脑屏障的局限性和推进cns靶向药物的开发至关重要。在本研究中，我们提出了一种新的bbp设计深度学习框架（DLF-BBP）。我们首先使用自注意生成对抗网络（Self-Attention Generative Adversarial Network， SAGAN）模型来生成新的候选bbp，并在计算机上进行评估，随后构建了基于卷积神经网络（Convolutional Neural Network， CNN）的预测模型，用于gan设计的bbp的初始筛选，实现了曲线下面积（AUC）为0.988，准确率为0.954。通过理化性质分析、分子对接和在线平台进行进一步的多步骤筛选。最后，通过体外血脑屏障渗透实验对过滤后的候选gan设计的血脑屏障进行验证，鉴定出三种具有高渗透能力的gan设计的血脑屏障屏障，其渗透能力与阳性对照利昔那肽相当。我们的研究为治疗神经系统疾病提供了新的可能性。

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引用次数: 0

Biodegradation characteristics, pathway elucidation, and toxicity assessment of oxytetracycline-polyethylene combined pollution by two Aspergillus fungi 两种曲霉真菌对土霉素-聚乙烯复合污染的生物降解特性、途径阐明及毒性评价

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-03-01 Epub Date: 2025-12-02 DOI: 10.1016/j.bej.2025.110027

Yuan Zhang, Shuicao Liu, Tingting Ding, Wanwan Hao, Shuwen Ren, Jingwen Min, Congyang Zou

The widespread use of antibiotics and agricultural microplastics has become a global environmental issue, yet studies on the degradation of their combined pollution remain limited. Here, two functional strains, Aspergillus sp. DL-1 and DL-2, selected from 10 isolates and identified via ITS sequencing and phylogenetic analysis, were investigated for their ability to degrade oxytetracycline (OTC)–polyethylene (PE) combined pollution under optimal conditions. The OTC-PE combined treatment with fungi exhibited superior degradation performance. The OTC degradation rate in the OPD2 treatment reached 96.33 ± 0.36 %, while the weight loss rates of PE in the OPD1 and OPD2 were 10.83 ± 0.53 % and 8.92 ± 0.51 %, respectively. Furthermore, the WCA of the PE films in the combined treatments remained consistently lower than in the single-PE treatments, accompanied by significant changes in surface morphology and structure. Under the treatment of the strains, OTC can be degraded through multiple pathways, including decarbonylation, decarboxylation, deamination, demethylation, and dehydration. Specifically, biodegradation by DL-1 and DL-2 markedly lowered the developmental toxicity, mutagenicity, and bioaccumulation potential of OTC and its intermediates, thereby alleviating the environmental toxicity burden. In parallel, PE cleaved the chain under the action of microbial enzymes to generate low molecular hydrocarbons, which are further oxidized to carboxylic acids and other intermediate products. These findings provide a novel approach for addressing compound pollution in agricultural environments.

抗生素和农业微塑料的广泛使用已成为一个全球性的环境问题，但对其复合污染的降解研究仍然有限。本文从10株分离物中筛选出两株功能菌株Aspergillus sp. DL-1和DL-2，通过ITS测序和系统发育分析对其进行了鉴定，研究了它们在最佳条件下降解土霉素(OTC) -聚乙烯（PE）复合污染的能力。OTC-PE与真菌联合处理表现出较好的降解性能。OPD2治疗的场外降解率达到96.33 ±0.36  %,而减肥的PE OPD1和OPD2 10.83±0.53   %和8.92±0.51  %,分别。此外，复合处理PE膜的WCA始终低于单一PE处理，并伴有表面形貌和结构的显著变化。在菌株的作用下，OTC可通过多种途径降解，包括脱碳、脱羧、脱胺、去甲基和脱水。具体而言，DL-1和DL-2的生物降解显著降低了OTC及其中间体的发育毒性、诱变性和生物蓄积潜力，从而减轻了环境毒性负担。同时，PE在微生物酶的作用下裂解链，生成低分子碳氢化合物，再被氧化为羧酸等中间产物。这些发现为解决农业环境中的复合污染提供了一种新的途径。

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引用次数: 0

Addressing temperature gradient challenge in scaling up solid-state fermentation: A strategy using thermophilic strains for biosurfactant production 解决固态发酵中温度梯度的挑战：利用嗜热菌株生产生物表面活性剂的策略

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-03-01 Epub Date: 2025-12-02 DOI: 10.1016/j.bej.2025.110037

Jose Bueno-Mancebo, Adriana Artola, Syeda Amna Farooq, Raquel Barrena, Teresa Gea

Solid-state fermentation (SSF) is a promising approach for sustainable bioproduction production, particularly when using organic waste as a substrate. However, temperature gradients inherent to large-scale SSF often reduce process efficiency. This study developed a strategy for designing of a lipopeptide biosurfactant production process, scaling SSF in packed-bed bioreactors by thermophilic strains using winterisation oil cake (WOC) and sugarcane molasses (MOL) as nutrient sources. Fermentations at lab-scale (0.5 L) and pilot-scale (50 L) demonstrated a robust and reproducible process despite temperature changes from microbial activity. Among the strains tested, Bacillus subtilis CBI-7S1 was the highest producer, with optimal substrates amounts of 24 g of WOC and 12 g of MOL, producing 24.9 mg of crude lipopeptides per gram of dry matter (DM) at 0.5 L scale. These conditions were reproduced at pilot scale, with concentrations from 12 to 24 mg g⁻¹ DM. Moisture content strongly influenced biosurfactant production, while oxygen consumption was a reliable monitoring parameter. Surface tension was evaluated under different pH, temperature, and salinity, and mass spectrometry identified surfactin, iturin, and fengycin congeners. These findings provide insights into overcoming temperature gradients during scale-up and show that thermophilic strains enable biosurfactant production under SSF at pilot scale. This approach enhances SSF technological maturity, supporting its broader use in sustainable production of targeted metabolites across diverse bioprocesses.

固态发酵（SSF）是一种很有前途的可持续生物生产方法，特别是当使用有机废物作为底物时。然而，大规模SSF固有的温度梯度往往会降低工艺效率。本研究开发了一种设计脂肽生物表面活性剂生产工艺的策略，通过嗜热菌株在填充床生物反应器中以冬季油饼（WOC）和甘蔗糖蜜（MOL）为营养来源进行SSF的规模化。实验室规模（0.5 L）和中试规模（50 L）的发酵表明，尽管微生物活性引起温度变化，但发酵过程稳健且可重复。在试验菌株中，枯草芽孢杆菌CBI-7S1的产量最高，其最佳底物WOC为24 g， MOL为12 g，在0.5 L的条件下，每克干物质（DM）的粗脂肽产量为24.9 mg。这些条件在中试规模上重现，浓度从12到24 mg g−1 DM。水分含量强烈影响生物表面活性剂的生产，而氧气消耗是可靠的监测参数。在不同的pH、温度和盐度下评估表面张力，质谱鉴定表面蛋白、iturin和fengycin同系物。这些发现为在放大过程中克服温度梯度提供了见解，并表明嗜热菌株能够在SSF中试规模下生产生物表面活性剂。这种方法提高了SSF技术的成熟度，支持其在不同生物过程中可持续生产目标代谢物的更广泛应用。

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引用次数: 0

Investigating the effect of media composition on growth and mAb production in CHO cells using a piecewise hybrid dFBA-PLS framework 使用分段混合dFBA-PLS框架研究培养基组成对CHO细胞生长和单克隆抗体产生的影响

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-03-01 Epub Date: 2025-11-21 DOI: 10.1016/j.bej.2025.110013

Zahra Negahban , Ali Ghodba , Anne Richelle , Chris McCready , Valerie Ward , Hector Budman

In this study, we present a hybrid modeling framework that integrates piecewise Partial Least Squares (PLS) regression with Dynamic Flux Balance Analysis (dFBA) to simulate and optimize Chinese Hamster Ovary (CHO) cell fed-batch culture. Twenty-four Ambr15 experiments were conducted to systematically vary feed and inoculum compositions. Time-resolved metabolite, biomass, and Monoclonal antibodies (mAb) concentrations were collected and modeled. The hybrid model achieved high prediction accuracy (Normalized Mean Squared Error (NMSE)

<

0.15 for most metabolites) and provided interpretable flux profiles. Multivariate analysis revealed consistent metabolic signatures tied to media formulation, where specific feed–inoculum combinations drove shifts in glycolysis, TCA cycle flux, and nitrogen metabolism. These insights demonstrate the model’s capacity to capture key metabolic adaptations and support data-driven media optimization in CHO cell culture.

在这项研究中，我们提出了一个混合建模框架，结合分段偏最小二乘（PLS）回归和动态通量平衡分析（dFBA）来模拟和优化中国仓鼠卵巢（CHO）细胞的间歇培养。为了系统地改变饲料和接种物的组成，进行了24次Ambr15试验。收集时间分辨代谢物、生物量和单克隆抗体（mAb）浓度并建立模型。该混合模型具有较高的预测精度（大多数代谢物的归一化均方误差（NMSE） & 0.15），并提供了可解释的通量谱。多变量分析揭示了与培养基配方相关的一致代谢特征，其中特定的饲料-接种组合驱动糖酵解、TCA循环通量和氮代谢的变化。这些见解证明了该模型在CHO细胞培养中捕获关键代谢适应和支持数据驱动媒体优化的能力。

引用次数: 0

An adsorption-crosslinking immobilization approach for enhancing the operational stability of D-allulose 3-epimerase immobilized on chitosan-modified resin 壳聚糖改性树脂固定化D-allulose 3- epimase的吸附-交联固定化方法

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-03-01 Epub Date: 2025-12-03 DOI: 10.1016/j.bej.2025.110039

Yingying Zheng , Hongwei Liu , Shumin Guo , Jingwen Fei , Junqing Wang , Ruiming Wang , Piwu Li

D-allulose is a naturally occurring rare ketohexose that serves as a potential sucrose substitute. In this study, we developed a novel adsorption-crosslinking immobilization strategy based on a chitosan (CTS)-modified resin. After screening 15 resin types, D213 and ZGA351 were selected as carriers, and the CTS-modified carriers were crosslinked with glutaraldehyde to immobilize D-allulose 3-epimerase (DAE; EC 5.1.3.30). CTS modification significantly enhanced the performance of the immobilized enzymes, with the activity recovery rates of D213@CTS@DAE and ZGA351@CTS@DAE increasing compared to the unmodified carriers. The thermal, pH, and storage stabilities of the immobilized enzymes were significantly higher than those of free enzymes. D213@CTS@DAE retained high initial activity across multiple recycling and reuse cycles and sustained productivity in a packed-bed reactor after 29 days of continuous operation. The carrier material could be efficiently regenerated and reused. After regeneration, the immobilized enzyme maintained a high activity retention rate after five cycles. The findings provide a green biocatalytic solution for D-allulose production, which enhances the practicality and commercial viability of the immobilized enzyme.

D-allulose是一种天然存在的稀有酮己糖，可作为潜在的蔗糖替代品。在本研究中，我们开发了一种基于壳聚糖（CTS）改性树脂的新型吸附-交联固定策略。筛选15种树脂后，选择D213和ZGA351作为载体，将cts修饰的载体与戊二醛交联固定D-allulose 3- epimase （DAE; EC 5.1.3.30）。CTS修饰显著提高了固定化酶的性能，与未修饰的载体相比，D213@CTS@DAE和ZGA351@CTS@DAE的活性回收率提高。固定化酶的热稳定性、pH稳定性和贮藏稳定性均显著高于游离酶。D213@CTS@DAE在连续运行29天后，在填料床反应器中保持了高的初始活性，并在多个回收和再利用周期中保持了持续的生产力。载体材料可以有效地再生和再利用。再生后，固定化酶在5个循环后保持较高的活性保留率。该研究结果为D-allulose的生产提供了一种绿色的生物催化解决方案，提高了固定化酶的实用性和商业可行性。

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引用次数: 0

Hybrid section-based modeling of gas-phase hydrodynamics in biotrickling filters: Influence of packing material on residence time distributions 基于混合截面的生物滴滤气相流体动力学建模：填料对停留时间分布的影响

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-03-01 Epub Date: 2025-12-13 DOI: 10.1016/j.bej.2025.110049

Felipe A. Carreño-López , Patricio A. Moreno-Casas , Felipe Scott , Alberto Vergara-Fernández , José Sierra-Pallares , Raúl Muñoz

In this study, the gas-phase hydrodynamics within a 6.5 L biotrickling filter were quantified using pulse-injection residence time distributions (RTDs) recorded with a cost-effective metal oxide (MOx) sensor. This was conducted at a constant gas flow rate of 8 L min⁻¹ across trickling liquid velocities (TLV) ranging from 0 to 10

{m h}^{- 1}

, using three different packings: polyurethane foam, Tri-Packs Jaeger spheres, and wood bark. A hybrid section-based model was developed and evaluated against two closed-closed axial dispersion variants (AD

_{c c}

+CSTR and AD

_{c c}

+PFR). Model discrimination using AIC/BIC and

R^{2}

indicated that the optimal model description is contingent upon the packing material and operating regime: the hybrid model most accurately represented foam and most wood cases, whereas AD

_{c c}

+CSTR was sufficient for spheres under irrigation, and AD

_{c c}

+PFR consistently underperformed. Employing the optimal model in each scenario, the Peclet number increased with TLV for PUF and spheres (with diminishing returns at the highest TLV), whereas wood exhibited a low Peclet number and non-monotonic behavior. By explicitly partitioning the mean residence time between the buffer zones and the bed, the hybrid model effectively separates the boundary hold-up from the core dispersion, yielding a more precise representation of the packed bed Peclet number. Overall, MOx-based RTD measurements, in conjunction with model comparison, offer a cost-effective in situ diagnostic tool for differentiating boundary mixing from bed transport, thereby informing packing selection and operating parameters in gas-phase biofiltration.

在这项研究中，利用脉冲注射停留时间分布（rtd）记录了一个具有成本效益的金属氧化物（MOx）传感器，定量了6.5 L生物滴滤器内的气相流体动力学。实验采用了三种不同的填料：聚氨酯泡沫、trii - packs Jaeger球体和木皮，在8 L min - 1的恒定气体流速下进行，滴液速度（TLV）范围为0到10 m h - 1。建立了一种基于切片的混合模型，并对两种闭式轴向弥散变量（ADcc+CSTR和ADcc+PFR）进行了评估。使用AIC/BIC和R2的模型判别表明，最佳模型描述取决于包装材料和操作制度：混合模型最准确地代表泡沫和大多数木材情况，而ADcc+CSTR对于灌溉下的球体足够，而ADcc+PFR一直表现不佳。采用最优模型，PUF和球体的Peclet数随TLV的增加而增加（在最高TLV时收益递减），而木材则表现出低Peclet数和非单调行为。通过显式划分缓冲区和床层之间的平均停留时间，混合模型有效地将边界占用率与核心色散分离开来，从而得到更精确的填充床佩莱特数表示。总的来说，基于mox的RTD测量，结合模型比较，提供了一种具有成本效益的现场诊断工具，用于区分边界混合和床层输送，从而为气相生物过滤的填料选择和操作参数提供信息。

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引用次数: 0

Bioconversion kinetics and valorisation of oil palm empty fruit bunches for biomethane production and lignin derived compounds 油棕空果束用于生物甲烷生产和木质素衍生化合物的生物转化动力学和价值评价

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-03-01 Epub Date: 2025-12-20 DOI: 10.1016/j.bej.2025.110053

Irnia Nurika , Eka Nur Shabrina , Nurul Azizah , Sri Suhartini , Guy C. Barker , Timothy D.H. Bugg

This study assesses the facultative anaerobe Comamonas testosteroni as a mild, chemical-free pre-treatment for valorising oil palm empty fruit bunches (OPEFB) into biomethane and lignin-derived aromatics. Incubation with 2 % (v/v) C. testosteroni for 7 days at 30 °C, reduced lignin to 15.67 % (33.42 % removal), while retaining high cellulose (53.48–56.19 %) and hemicellulose (up to 16.21 %). Pre-treated OPEFB showed 20 % weight loss (vs 8 % in controls) and a 51 % rise in total soluble phenols, evidencing active lignin depolymerisation. GC–MS of liquor and solids qualitatively confirmed lignin breakdown and identified representative low-molecular-weight products (e.g., phenol, betulin, acetic acid and benzoxazole), supporting co-product potential. In biochemical methane potential tests, the pre-treated residue achieved a specific methane potential of 0.173 m³ CH₄ kg⁻¹ VS, an 85 % increase over the abiotic residue and higher than non-treated OPEFB. Kinetic fitting with Transference, Modified Gompertz and Logistic models yielded good agreement (R² up to 0.92), with Modified Gompertz best capturing cumulative methane production and the shortest lag phases. A simple energy check indicates a favourable margin: the incremental methane (ΔSMP ≈ 0.08 m³ CH₄ kg⁻¹ VS) equates ∼0.8 kWh kg⁻¹ VS, whereas estimated mixing for pre-treatment is ∼0.017–0.034 kWh kg⁻¹ VS. Overall C. testosteroni pre-treatment enables dual valorisation (biomethane plus aromatics) under mesophilic conditions, offering a practical route for integrated OPEFB biorefineries.

本研究评估了兼性厌氧菌睾酮单胞菌作为一种温和的、无化学物质的预处理，用于将油棕空果束（OPEFB）转化为生物甲烷和木质素衍生的芳烃。与2 % (v/v) C. steroni在30°C下孵育7天，木质素降至15.67 %（去除率33.42 %），同时保留高纤维素（53.48-56.19 %）和半纤维素（高达16.21 %）。预处理后的OPEFB重量减轻了20% %（对照组为8% %），可溶性酚总含量增加了51% %，证明木质素解聚活性。液相和固相的GC-MS定性证实了木质素的分解，并鉴定了具有代表性的低分子量产物（如苯酚、白桦脂、乙酸和苯并恶唑），支持了副产物的潜力。在生化甲烷电位测试中，预处理残渣的比甲烷电位为0.173 m³ CH4 kg⁻¹ VS，比非生物残渣增加85 %，高于未经处理的OPEFB。与Transference、Modified Gompertz和Logistic模型的动力学拟合结果吻合良好（R2高达0.92），其中Modified Gompertz模型最能捕获累积甲烷产量，滞后期最短。一个简单的能量检查表明了一个有利的余量：增加的甲烷（ΔSMP≈0.08 m³CH₄kg⁻¹VS）相当于0.8 kWh kg⁻¹ VS，而预处理的估计混合量为0.017-0.034 kWh kg⁻¹ VS。总的来说，C. testosterone预处理可以在中温条件下实现双重增值（生物甲烷和芳烃），为集成OPEFB生物精炼厂提供了一条实用的途径。

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引用次数: 0

Insights into microbial and sorptive regulation of chlorpyrifos-bispyribac dissipation in floating treatment wetlands 漂浮处理湿地中毒死蜱-双吡虫蜱耗散的微生物和吸附调控研究

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-03-01 Epub Date: 2025-12-10 DOI: 10.1016/j.bej.2025.110045

Kashaf Farooq , Muhammad Arslan , Samina Anwar , Maryam Noor , Muhammad Afzal

Agricultural runoff remains one of the most persistent threats to water quality worldwide. Floating wetlands (FWs), when designed appropriately, offer a promising nature-based solution. Here, we show how biologically and sorptively active FWs can remove two agrochemicals—chlorpyrifos (CPF) and bispyribac‑sodium (BIS), under response surface methodology (RSM)‑backed optimal operation (pH 8; 35 °C; 10 mg L⁻¹ each; 1 % glucose). FWs built with Phragmites australis (common reed) were amended with a defined consortium (CB2H, 1 % v/v), plant‑derived biochar (1.5 % w/v), biochar‑immobilized CB2H (1.5 % w/v), and nutrients (N 25 mg L⁻¹, P 25 mg L⁻¹, K 20 mg L⁻¹). CPF and BIS declined exponentially, fitting pseudo‑first‑order kinetics with adsorption component (high S, K_d in the immobilized system). CPF disappeared fastest in the consortium‑only and biochar‑immobilized treatments (k = 0.07 and 0.09 day⁻¹), resulting in > 99 % removal (>9.9 mg L⁻¹) by day 20; BIS peaked at > 84 % (8.4 mg L⁻¹) with immobilized cells. FTIR shifts (∼2920–2840, 2800 cm⁻¹) and new CO bands (1600–1800 cm⁻¹) indicated hydrogen bonding in the Phragmites biochar. Approximately 40 % of the CPF loss in the controls was abiotic (sorption/photolysis/hydrolysis). Chemical oxygen demand and ancillary pollutants also declined. Enhanced performance was supported by microbial colonization within biochar pores. The study provides key design constants (k, K_d), positioning engineered FWs as a scalable nature-based technology for pesticide-laden agricultural runoff.

农业径流仍然是全球水质最持久的威胁之一。如果设计得当，浮动湿地（FWs）是一种很有前途的基于自然的解决方案。在这里，我们展示了生物和吸附活性的FWs如何在响应面法（RSM）支持的最佳操作下（pH值8；35°C； 10 mg L⁻¹各；1 %葡萄糖）去除两种农药——毒死蜱（CPF）和双嘧菌酯钠（BIS）。美国鱼类和野生动物管理局用芦苇南极光(芦苇)修改与定义财团(CB2H 1 % v / v),植物-衍生生物炭(1.5 % w / v),生物炭-固定化CB2H(1.5 % w / v)和养分(N 25 mg  L⁻¹,P 25 毫克 L⁻¹,K 20 mg  L⁻¹)。CPF和BIS呈指数级下降，拟合吸附组分的准一级动力学（固定体系中S、Kd较高）。在纯联合治疗和生物炭固定治疗中，CPF消失得最快（k = 0.07和0.09天⁻¹），到第20天，CPF就被清除了>； 99% % （>9.9 mg L⁻¹）；在固定细胞时，BIS的峰值为>； 84 %（8.4 mg L⁻¹）。FTIR变化（~ 2920 - 2840,2800 cm⁻¹）和新的CO波段（1600-1800 cm⁻¹）表明芦苇生物炭中存在氢键。在对照组中，大约40% %的CPF损失是非生物的（吸收/光解/水解）。化学需氧量和辅助污染物也有所下降。生物炭孔隙内的微生物定殖支持了性能的提高。该研究提供了关键的设计常数（k, Kd），将工程FWs定位为一种可扩展的基于自然的农药农业径流技术。

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引用次数: 0

Improvement of furfural tolerance and butanol production in Clostridium beijerinckii ART44 through overexpression of Class I heat shock proteins 通过过表达I类热休克蛋白提高贝氏梭菌ART44的糠醛耐受性和丁醇产量

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-03-01 Epub Date: 2025-12-11 DOI: 10.1016/j.bej.2025.110047

Chaoyue Shi , Caolin Liu , Xin Cheng, Yujie Jing, Qinghua Zhang, Wenjie Lai, Hanguang Li

The conversion of lignocellulosic hydrolysate into butanol offers a significant approach to reduce environmental pollution and the greenhouse effect. However, the presence of inhibitors in the hydrolysate restricts its utilization efficiency. In this study, we investigated the role of Class I heat shock proteins (HSPs) in Clostridium beijerinckii ART44 under furfural stress. Notably, the grpE gene was identified as being significantly upregulated under this condition. Therefore, to enhance the furfural tolerance and butanol production, the grpE gene was overexpressed. With this effort, the resulting recombinant strain, named as ART44/grpE, exhibited a 73.75 % increase in butanol production (5.34 ± 0.29 g/L) and a 53.99 % increase in total solvent production (7.67 ± 0.16 g/L) under 3.0 g/L furfural stress, compared to the wild-type strain ART44. Furthermore, mechanistic studies revealed that grpE overexpression reduced cell surface hydrophobicity, minimized the leakage of intracellular macromolecules, and increased the proportion of saturated fatty acids in the cell membrane. These alterations contributed to enhancing cell viability, improving butanol biosynthesis enzyme activity, and reducing membrane permeability under furfural stress. Collectively, the results demonstrate that overexpressing grpE in C. beijerinckii ART44 is an effective and attainable approach to obtain a high furfural tolerance strain, and then improving its butanol production from lignocellulose hydrolysate.

木质纤维素水解产物转化为丁醇为减少环境污染和温室效应提供了一条重要途径。然而，水解产物中抑制剂的存在限制了其利用效率。在本研究中，我们研究了一类热休克蛋白（HSPs）在糠醛胁迫下在贝氏梭菌ART44中的作用。值得注意的是，在这种情况下，grpE基因被鉴定为显著上调。因此，为了提高糠醛耐受性和丁醇产量，grpE基因被过表达。这个努力,由此产生的重组菌株,命名为ART44 / grpE,表现出73.75 %提高丁醇生产(5.34 ±0.29  g / L)和53.99 %增加溶剂总产量(7.67 ±0.16  g / L)低于3.0 g / L糠醛压力,与野生型菌株ART44相比。此外，机制研究表明，grpE过表达降低了细胞表面疏水性，减少了细胞内大分子的渗漏，增加了细胞膜中饱和脂肪酸的比例。这些变化有助于提高细胞活力，提高丁醇生物合成酶活性，降低糠醛胁迫下的膜通透性。综上所述，在C. beijerinckii ART44中过表达grpE是获得高糠醛耐受性菌株，进而提高其木质纤维素水解丁醇产量的有效途径。

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