Biochemical Engineering Journal最新文献_第10页

Insight into the enhanced cAMP synthesis using Arthrobacter sp. CCTCC 2013431 under polyphosphate addition condition based on physiological and transcriptome analyses 基于生理和转录组分析，深入了解在多磷酸盐添加条件下使用Arthrobacter sp. CCTCC 2013431增强cAMP合成

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-03-01 Epub Date: 2025-12-15 DOI: 10.1016/j.bej.2025.110048

Zhigang Li , Nanxun Lu , Ruifang Li , Wei Liu , Chang Li , Zhonghua Zhang , Tianyou Yang

The insufficient supply of ATP limited cAMP synthesis during fermentations using Arthrobacter sp. CCTCC 2013431. cAMP fermentations with/without hexametaphosphate addition were conducted in a 7 L bioreactor and the underlying mechanism for enhanced cAMP biosynthesis under hexametaphosphate addition condition was investigated. Due to the addition of 2 g/L-broth sodium hexametaphosphate at 24 h, cAMP content reached 3.64 g/L, with an increment of 33.82 % when compared with control. The transcriptome analysis results revealed that 227 genes were up-regulated and 265 genes were down-regulated significantly in response to hexametaphosphate addition. For glycometabolism, the transcription levels of key enzymes genes involved with cAMP synthesis were enhanced significantly, and for energy metabolism, the transcription levels of genes associated with electron transport chain and polyphosphate kinase gene were increased significantly by which sufficient carbon skeleton and ATP were provided for cAMP biosynthesis. In addition, transcription levels of most reductase genes were up regulated significantly suggesting that intracellular antioxidant capacity was improved effectively by hexametaphosphate. Finally, activities of key enzymes together with intracellular contents of ROS, MDA and key metabolites were detected and the results were in accordance to those of transcriptome analysis. It could be concluded that hexametaphosphate relieved severe oxidative stress, strengthened energy metabolism and redistributed the carbon flux among different pathways resulting in enhanced cAMP synthesis and decreased byproduct generation.

在利用关节杆菌sp. CCTCC 2013431发酵过程中，ATP供应不足限制了cAMP的合成。在一个7 L的生物反应器中进行了有/没有六偏磷酸的cAMP发酵，并研究了六偏磷酸条件下cAMP生物合成增强的潜在机制。24 h加入2 g/L-六偏磷酸钠，cAMP含量达到3.64 g/L，比对照增加33.82 %。转录组分析结果显示，六偏磷酸酯添加显著上调了227个基因，下调了265个基因。糖代谢方面，参与cAMP合成的关键酶基因转录水平显著提高；能量代谢方面，电子传递链和多磷酸激酶相关基因转录水平显著提高，为cAMP生物合成提供了充足的碳骨架和ATP。此外，大多数还原酶基因的转录水平显著上调，表明六偏磷酸有效提高了细胞内抗氧化能力。最后检测关键酶活性、细胞内ROS、MDA及关键代谢物含量，结果与转录组分析结果一致。综上所述，六偏磷酸盐缓解了严重的氧化应激，加强了能量代谢，并在不同途径之间重新分配了碳通量，从而提高了cAMP的合成，减少了副产物的产生。

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引用次数: 0

Hydrocyclone-enhanced nutrient removal and sludge densification in activated sludge system: Insights into microbial response 活性污泥系统中水力旋流器增强的营养物去除和污泥致密化：对微生物反应的见解

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-03-01 Epub Date: 2025-12-15 DOI: 10.1016/j.bej.2025.110051

Wentao Xu , Shasha Liu , Mei Sun , Shijie Sun , Jiao Wang , Zhiming Bian , Qingyun Zhang , Changsheng Peng

Sludge densification is a promising technology to enhance nutrient removal in existing municipal wastewater treatment plants (WWTPs). In this study, the graded-device hydrocyclone was installed into activated sludge system to assist sludge densification and disclose its microbial response mechanism. Higher nutrient removal (94.2 % for COD, 97.2 % for NH₄⁺-N, 82.3 % for TN, 92.4 % for TP, respectively) were achieved in R2 reactor incorporating hydrocyclone, and these effluent indicators met the wastewater first class A discharge standards of China (GB18918–2002). The hydraulic shear forces of hydrocyclone promoted the sludge transformation from loose flocs to dense particles (average size of 292 μm), sludge settleability (relative hydrophobicity of 79.1 %, SVI₃₀ of 60 mL/g), and the secretion of extracellular polymeric substances (EPS of 98.89 mg/g-SS, protein/polysaccharide ratio of 1.78). Ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, denitrifying bacteria and polyphosphate-accumulating organisms were effectively enriched. These functional bacteria actively participated in membrane transport, signal transduction, carbon metabolism, nitrogen metabolism, phosphonate and phosphinate metabolism, providing the core driving force for long-term stable nutrient removal. These findings provided feasibility and mechanism support for hydrocyclone in enhancing nutrient removal, with a view to achieving cost reduction and efficiency benefits via sludge densification in industrial applications at full-scale WWTPs within the environmental field.

污泥致密化是一种很有前途的技术，可以提高现有城市污水处理厂的营养物去除率。本研究将分级装置水力旋流器安装在活性污泥系统中，辅助污泥致密化，揭示其微生物响应机理。采用水力旋流器的R2反应器，COD去除率为94.2 %，NH4+-N去除率为97.2% %，TN去除率为82.3 %，TP去除率为92.4 %，出水指标均达到中国废水一级A排放标准（GB18918-2002）。水力旋流器的水力剪切力促进了污泥由松散絮体向致密颗粒（平均粒径为292 μm）的转化，污泥的沉降性（相对疏水性为79.1% %，SVI30为60 mL/g），以及细胞外高分子物质的分泌（EPS为98.89 mg/g- ss，蛋白/多糖比为1.78）。氨氧化菌、亚硝酸盐氧化菌、反硝化菌和聚磷菌得到有效富集。这些功能性细菌积极参与膜转运、信号转导、碳代谢、氮代谢、膦酸盐和膦酸盐代谢，为长期稳定的营养物去除提供核心动力。这些发现为水力旋流器加强营养物去除提供了可行性和机制支持，以期在环境领域的大规模污水处理厂的工业应用中通过污泥致密化实现成本降低和效率提高。

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引用次数: 0

In situ directed evolution of γ-glutamyl kinase in Corynebacterium glutamicum S9114 by innovative EvolvR-mediated mutagenesis and rare codon-based selection to increase L-proline production 利用创新性evolvr介导的诱变和基于罕见密码子的选择，谷氨酸棒状杆菌S9114的γ-谷氨酰基激酶原位定向进化以增加l -脯氨酸的产量

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-02-01 Epub Date: 2025-10-17 DOI: 10.1016/j.bej.2025.109982

Jian-Zhong Xu , Feng Zhang , Kai Wang , Shuo Wan , Wei-Guo Zhang

An efficient evolution and selection system is vital for constructing the high added-value products hyper-producers in fermentation industry. Herein, an innovative EvolvR-mediated mutagenesis was used to diversify the γ-glutamyl kinase (GK)-coding gene proB in C. glutamicum S9114 and an optimized rare codon-based selection was used to isolate the L-proline high-yielding strain with optimal GK variant. Triple-mutant GK^{E74K/G149K/V150M} was identified with high substrate affinity and catalytic efficiency for producing L-proline. The resultant strain C. glutamicum Pro-7 produced 94.2 ± 5.6 g/L of L-proline with productivity of 1.96 g/(L·h) and a yield of 0.316 g/g. Subsequently, the molecular mechanisms involved in high L-proline production in strain Pro-7 was discovered, indicating that ATP and NADPH supply are also the key factors for the L-proline biosynthesis besides GK. Thus, in situ directed evolution of key genes in genome by EvolvR-mediated mutagenesis and rare codon-based selection provides a new direction and reference to construct industrial-strength strains.

高效的进化和选择系统是构建发酵工业高附加值产品超级生产者的关键。本文采用创新性的evolvr介导诱变技术，对C. glutamicum S9114中γ-谷氨酰基激酶（GK）编码基因probb进行了突变，并采用优化的稀有密码子选择方法分离出了具有最佳GK变异的l -脯氨酸高产菌株。三突变体GKE74K/G149K/V150M对l -脯氨酸具有较高的底物亲和力和催化效率。菌株C. glutamum Pro-7的L-脯氨酸产量为94.2 ± 5.6 g/L，产率为1.96 g/(L·h)，产率为0.316 g/g。随后发现菌株Pro-7高产l -脯氨酸的分子机制，表明除了GK外，ATP和NADPH的供应也是l -脯氨酸生物合成的关键因素。因此，通过evolvr介导的突变和基于稀有密码子的选择对基因组关键基因进行原位定向进化，为构建工业强度菌株提供了新的方向和参考。

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引用次数: 0

Treatment of real aquaculture wastewater using marine Chlorella: Pollutant removal and microbial community dynamics 用海洋小球藻处理真实水产养殖废水：污染物去除和微生物群落动态

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-02-01 Epub Date: 2025-10-31 DOI: 10.1016/j.bej.2025.109987

Shenwei Cheng , Yanqing Sheng

This study comprehensively investigated the nutrient removal mechanisms of Chlorella sp. in authentic aquaculture wastewater, while also examining the dynamic shifts within its symbiotic microbial community. The results showed that the microalgae exhibited robust growth in the wastewater, effectively reducing concentrations of ammonium nitrogen, nitrate nitrogen, and phosphate with removal efficiencies of 95.0 %, 93.2 %, and 91.7 %, respectively. Fluctuations in pH during the initial cultivation period indicated a cyclical interplay between heterotrophic microbial activity and microalgal photosynthesis. In the later stages, enhanced synthesis of Chlorella carotenoids was correlated with deteriorating water quality and the onset of oxidative stress. Initially, the microbial community was dominated by organic matter degradation, nitrate reduction, and fermentation. However, as cultivation progressed, metabolic activities shifted towards the photoassimilation of inorganic nutrients. The Chlorella sp. symbiotic system facilitated a rapid succession of the microbial community, which was characterized by frequent bacterial population changes, ultimately leading to a unique, temporally stable community structure. The initially diverse microbial population was progressively supplanted by more adaptive bacterial strains, achieving a steady state by the seventh day. Notably, Alcaligenaceae demonstrated exceptional adaptability compared to other genera, underscoring its pivotal role within the Chlorella sp. symbiotic system. This research provides valuable theoretical insights into algal-bacterial interactions and highlights their potential for application in water quality remediation.

本研究全面探讨了小球藻在真实养殖废水中的营养去除机制，同时考察了其共生微生物群落的动态变化。结果表明，微藻在废水中生长旺盛，能有效降低铵态氮、硝态氮和磷酸盐的浓度，去除率分别为95.0 %、93.2 %和91.7 %。培养初期pH值的波动表明异养微生物活性与微藻光合作用之间存在周期性相互作用。在后期，小球藻类胡萝卜素合成的增加与水质恶化和氧化应激的发生有关。最初，微生物群落以有机物降解、硝酸盐还原和发酵为主。然而，随着栽培的进行，代谢活动转向无机养分的光同化。小球藻共生系统促进了微生物群落的快速演替，其特征是细菌种群的频繁变化，最终形成了一个独特的、暂时稳定的群落结构。最初多样化的微生物种群逐渐被适应性更强的菌株所取代，在第7天达到稳定状态。值得注意的是，与其他属相比，Alcaligenaceae表现出特殊的适应性，强调了它在小球藻共生系统中的关键作用。该研究为藻-细菌相互作用提供了有价值的理论见解，并强调了它们在水质修复中的应用潜力。

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引用次数: 0

Biotransformation from rutin to quercetin in tomato by-products using almond β-glucosidase 利用杏仁β-葡萄糖苷酶将番茄副产品中的芦丁转化为槲皮素

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-02-01 Epub Date: 2025-10-06 DOI: 10.1016/j.bej.2025.109955

Jared Mauricio López-Téllez, María del Pilar Cañizares-Macías

Within the circular economy, quercetin is a high-value polyphenol with significant food and pharmaceutical applications, which can be chemically or enzymatically produced from rutin. This study proposes an efficient enzymatic strategy using both free and immobilized β-glucosidase for rutin hydrolysis. When standard rutin was tested hydrolysis rates reached 80.69 ± 2.89 % (free enzyme) and 82.33 ± 3.45 % (immobilized enzyme), with conversion to quercetin at 49.14 ± 2.63 % and 55.22 ± 1.90 %, respectively. Application to tomato peels and seeds showed nonetheless very promising results with rutin hydrolysis rates between 29.14 ± 2.77 % (immobilized enzyme) and 35.86 ± 3.11 % (free enzyme) and quercetin conversion from 19.31 ± 2.16 % to 21.09 ± 3.63 %. Nonetheless, quercetin content and antioxidant capacity increased closely by 32.00 % and 18.00 %, respectively, after hydrolysis. These findings introduce a rapid, sustainable, and cost-effective enzymatic process for enhancing the bioactive potential of tomato by-products, providing a valuable tool for agro-industrial applications and contributing to the valorization of food waste.

在循环经济中，槲皮素是一种高价值的多酚，具有重要的食品和制药应用，可以由芦丁化学或酶法生产。本研究提出了一种利用游离和固定化β-葡萄糖苷酶水解芦丁的有效方法。当标准测试芦丁水解率达到80.69 ±2.89  %(自由酶)和82.33 ±3.45  %(固定化酶),转换为槲皮素在49.14 ±2.63   %和55.22±1.90  %,分别。应用番茄皮和种子之间显示还是非常有前途的结果与芦丁水解率29.14 ±2.77   %(固定化酶)和35.86±3.11  %(自由酶)和槲皮素转换从19.31 ±2.16  % 21.09 ±3.63  %。水解后槲皮素含量和抗氧化能力分别提高了32.00 %和18.00 %。这些发现为提高番茄副产品的生物活性潜力提供了一种快速、可持续、经济的酶促过程，为农业工业应用提供了有价值的工具，并有助于食物垃圾的价值化。

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引用次数: 0

Multiscale CFD-metabolic modeling reveals hybrid impeller configurations mitigate substrate and oxygen starvation in industrial bioreactors 多尺度cfd -代谢模型揭示了混合叶轮配置减轻了工业生物反应器中的底物和缺氧

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-02-01 Epub Date: 2025-10-08 DOI: 10.1016/j.bej.2025.109956

Qingfeng Gu , Junxiong Yu , Yongqiang Liu , Yongbo Wang , Yingping Zhuang , Chao Li

Industrial-scale aerobic fermentations face persistent challenges from spatiotemporal substrate and oxygen gradients, which impair microbial productivity during scale-up. To address this, we establish a multiscale Computational Fluid Dynamics (CFD)-metabolic modeling framework to evaluate hybrid radial-axial impeller configurations in a 156 m³ stirred tank reactor. Compared to homogeneous all-radial and all-axial systems, hybrid configurations maintain superior balance in gas-liquid mixing, mass transfer, and power consumption through synergistic flow interactions. Integrated metabolic regime analysis and Lagrangian particle tracking confirm that the hybrid radial-axial impeller configurations effectively alleviate glucose and dissolved oxygen starvation. Especially, the C-K-R-R configuration, which uses a CD-6 impeller at the bottom, an axial-flow KSX impeller on the second level, and two Rushton turbines above, demonstrates optimal performance with 41.1 % non-starvation zone coverage (3.3 times all-axial impeller configurations) and 64.9 % residence time (1.6 times all-radial impeller configurations). This work establishes a multiscale optimization framework bridging bioreactor hydrodynamics with microbial metabolism, providing actionable strategies for scaled-up bioprocess design and intensification of industrial fermentation systems.

工业规模的好氧发酵面临着来自时空底物和氧梯度的持续挑战，这些挑战会在扩大规模期间损害微生物的生产力。为了解决这个问题，我们建立了一个多尺度计算流体动力学(CFD)-代谢建模框架来评估156 m³ 搅拌槽式反应器中径向-轴向混合叶轮的配置。与均匀的全径向和全轴向系统相比，混合配置通过协同流动相互作用，在气液混合、传质和功耗方面保持了更好的平衡。综合代谢状态分析和拉格朗日粒子跟踪证实，混合径向-轴向叶轮构型有效缓解葡萄糖和溶解氧饥饿。特别是C-K-R-R配置，底部使用CD-6叶轮，第二级使用轴流式KSX叶轮，上面使用两台Rushton涡轮机，具有最佳性能，非饥饿区覆盖率为41.1 %（全轴向叶轮配置的3.3倍），停留时间为64.9 %（全径向叶轮配置的1.6倍）。本研究建立了一个连接生物反应器流体动力学与微生物代谢的多尺度优化框架，为工业发酵系统的规模化生物工艺设计和强化提供了可行的策略。

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引用次数: 0

Bioremediation of diesel-contaminated saline soil through halotolerant-lipolytic fungal consortium and humic acid 耐盐溶脂真菌联合体和腐植酸对柴油污染盐渍土的生物修复作用

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-02-01 Epub Date: 2025-10-15 DOI: 10.1016/j.bej.2025.109969

Ahmed Sarfaraz , Sumbal Sajid , Qingming Zhang , Hengxing Zhang , Lin Lin , Wenfang Chen , Farhan Nabi , Syed Muhammad Mustajab Shah , Obey Kudakwashe Zveushe , Amal Mohamed Omer , Ying Han

Rapid urbanization has resulted in high diesel oil demand and consumption. Such processes are mostly carried out near coastal areas, where accidental oil spills occur during storage and handling, resulting in diesel-contaminated soils. Usually, soil microbial populations can remediate such contamination. However, the remediation of diesel-contaminated saline soils is challenging, as high salt concentrations inhibit the growth and activity of many hydrocarbon-degrading microbes, thereby limiting their remediation potential. In the current study, a combined approach was employed to remediate diesel-contaminated saline soil through bioaugmentation of a halotolerant-lipolytic fungal consortium (HLFC) and humic acid (HA) to alleviate the phytotoxicity of diesel and salt in Lolium perenne in the greenhouse experiment. The highest total petroleum hydrocarbon (TPH) degradation was achieved by the combined application of HLFC-HA (78.1 %), followed by HLFC (74.1 %), HA (65.2 %), and control (CK) (50.8 %). Plants displayed significant stress in diesel-contaminated saline soil. However, the combined application significantly reduced stress, plants showed better growth, photosynthesis, chlorophyll content, and lower antioxidant enzyme activity. Microbial diversity analysis showed that the relative abundances of the fungal genera Scopulariopsis and Aspergillus were significantly high in HLFC and HLFC-HA amended soils. Besides that, soil enzyme activity, including lipase and laccase, significantly increased in HLFC and HLFC-HA-amended soil. These findings demonstrated that HLFC-HA could be a beneficial strategy for the remediation of such oil-contaminated sites.

快速的城市化导致了柴油的高需求和高消耗。这些过程大多在沿海地区附近进行，在储存和处理过程中会发生意外的石油泄漏，导致柴油污染土壤。通常，土壤微生物种群可以修复这种污染。然而，修复柴油污染的盐碱地是具有挑战性的，因为高浓度的盐抑制了许多碳氢化合物降解微生物的生长和活性，从而限制了它们的修复潜力。本研究在温室试验中，采用耐盐溶脂真菌联合体（HLFC）和腐植酸（HA）联合修复柴油污染盐碱地的方法，减轻了柴油和盐对黑麦草（Lolium perenne）的植物毒性。HLFC-HA对总石油烃（TPH）的降解效果最好（78.1 %），其次是HLFC（74.1 %）、HA（65.2% %）和对照（CK）（50.8 %）。在柴油污染的盐渍土壤中，植物表现出明显的胁迫。但配施显著降低了胁迫，植株生长、光合、叶绿素含量均有所提高，抗氧化酶活性降低。微生物多样性分析表明，在HLFC和HLFC- ha改性土壤中，真菌属Scopulariopsis和Aspergillus的相对丰度显著较高。此外，HLFC和HLFC- ha处理的土壤酶活性显著提高，包括脂肪酶和漆酶。这些发现表明，高氟化碳- ha可能是一种有益的石油污染场地修复策略。

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引用次数: 0

Rapid lactic acid fermentation of vegetable waste in liquid-phase systems 植物废弃物液相系统快速乳酸发酵研究

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-02-01 Epub Date: 2025-11-01 DOI: 10.1016/j.bej.2025.109989

Han Zhang , Xue Song , Jie Zhang , Yongde Liu , Tianrun Bai , Feiyue Wang , Guihua Yan , Longjie Xiao , Tianzeng Jin , Jihong Zhao

In this study, vegetable waste liquid (VWL) was used as a substrate to produce lactic acid (LA) through anaerobic fermentation. The effects of inoculum size, carbon-to-nitrogen ratio (C/N ratio), temperature, and initial pH on LA yield and microbial succession were systematically investigated. All parameters significantly influenced LA production, with optimized conditions (12 % inoculum, initial pH of 6, C/N ratio of 20, 35 °C) yielding maximum LA production (11.76 g/L) within 16 h, corresponding to a 53.26 % glucose conversion rate. Excessive inoculum or deviation from optimal pH inhibited LA synthesis, whereas an appropriate C/N ratio enhanced microbial activity and substrate utilization. Lactobacillus plantarum was identified as the dominant species, with its relative abundance increasing from 64.14 % to 70.33 % at 16 h before stabilizing at 71.47 % at 24 h. Its metabolic dominance strongly correlated with rapid acidification, indicating its key role in efficient LA biosynthesis. Overall, precise fermentation parameter regulation effectively alleviated LA feedback inhibition and shortened the fermentation cycle to less than 24 h, offering a sustainable approach for converting vegetable waste into valuable biochemicals.

本研究以植物废液（VWL）为底物，通过厌氧发酵生产乳酸（LA）。系统研究了接种量、碳氮比（C/N）、温度和初始pH对LA产量和微生物演替的影响。所有参数均显著影响LA的产量，优化条件（接种量为12 %，初始pH为6，C/N比为20,35°C）在16 h内产生最大LA产量（11.76 g/L），对应的葡萄糖转化率为53.26% %。过量接种或偏离最佳pH会抑制LA合成，而适当的C/N比率则会增强微生物活性和底物利用率。植物乳杆菌（Lactobacillus plantarum）为优势菌种，其相对丰度在16 h时从64.14 %增加到70.33 %，在24 h时稳定在71.47 %。其代谢优势与快速酸化密切相关，表明其在高效LA生物合成中起关键作用。总体而言，精确的发酵参数调节有效地缓解了LA反馈抑制，并将发酵周期缩短至24 h以下，为将蔬菜废弃物转化为有价值的生化物质提供了一条可持续的途径。

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引用次数: 0

Temperature-tailored cellulose-rich Cladophora sp. hydrochar for selective malachite green adsorption from multi-dye systems 温度定制的富含纤维素的Cladophora sp.碳氢化合物对多染料系统中孔雀石绿的选择性吸附

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-02-01 Epub Date: 2025-10-30 DOI: 10.1016/j.bej.2025.109986

Muhammad Badaruddin , Laila Hanum , Elda Melwita , Sahrul Wibiyan , Yulizah Hanifah , Aldes Lesbani

This study presents the selective adsorption of malachite green (MG) from multi-dye mixtures using hydrochar derived from Cladophora sp. (C) via hydrothermal carbonization (HTC) at 150°C (C150), 200°C (C200), 250°C (C250), and 300°C (C300). Structural characterizations (XRD, FTIR, SEM-EDS, BET) confirmed enhanced porosity and surface functionalization with increasing HTC temperature. C200 exhibited the highest BET surface area (15.84 m²/g), while C300, with the largest pore size (18.27 nm), showed the highest MG adsorption capacity (204.1 mg/g), outperforming C (30.03 mg/g). Selective adsorption tests using ternary anionic-cationic dye mixtures revealed strong affinity toward cationic dyes, with MG showing 95.5 % removal by C300. Kinetic studies fitted the pseudo-second-order model, suggesting chemisorption dominance; the Avrami model also correlated well. Increasing Avrami rate constant (k₃) and decreasing exponent (n) with HTC temperature indicate a transition from diffusion to surface-controlled chemisorption. Equilibrium data followed the Sips isotherm, reflecting mixed monolayer–multilayer adsorption on moderately heterogeneous surfaces. The rising Sips constant (Kₛ) with temperature confirmed an endothermic process consistent with thermodynamic findings, which also verified spontaneity. Regeneration studies showed C300 retained > 50 % efficiency after three cycles, demonstrating stability and reusability. FTIR, XRD, and XPS analyses before and after MG adsorption confirmed interactions via electrostatic attraction, hydrogen bonding, and π–π interactions.

本研究介绍了从Cladophora sp. (C)衍生的碳氢化合物（HTC）在150°C (C150), 200°C (C200), 250°C （C250）和300°C （C300）下通过水热碳化（HTC）从多种染料混合物中选择性吸附孔雀石绿（MG）。结构表征（XRD， FTIR, SEM-EDS， BET）证实，随着HTC温度的升高，孔隙率和表面功能化增强。C200的BET表面积最大（15.84 m²/g）， C300的吸附量最大（18.27 nm），达到204.1 MG /g，优于C（30.03 MG /g）。使用三元阴离子-阳离子染料混合物的选择性吸附试验表明，对阳离子染料有很强的亲和力，C300对MG的去除率为95.5 %。动力学研究符合伪二阶模型，表明化学吸附优势；Avrami模型也有很好的相关性。Avrami速率常数（k₃）随着HTC温度的升高而增大，指数(n)随着HTC温度的升高而减小，表明由扩散向表面控制的化学吸附转变。平衡数据遵循Sips等温线，反映了在中等非均质表面上的混合单层-多层吸附。Sips常数（Kₛ）随温度的升高证实了一个与热力学结果一致的吸热过程，这也证实了自发性。再生研究表明，C300在三次循环后保持了>； 50 %的效率，表现出稳定性和可重复使用性。吸附MG前后的FTIR、XRD和XPS分析证实了静电吸引、氢键和π -π相互作用。

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引用次数: 0

Numerical simulation of catalytic enhancement in wall-coated enzyme-catalyzed micro-channel via geometric design 基于几何设计的壁涂酶催化微通道催化强化数值模拟

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-02-01 Epub Date: 2025-10-01 DOI: 10.1016/j.bej.2025.109947

Yiruo He , Jun Liang , Huiting Xu , Zhixi Zhang , Weiyi Su , Yuqi Hu , Na Wang , Xiong Yu , Honghai Wang

Microfluidics is widely used in enzyme biotechnology. Wall-coated immobilized enzyme micro-channels (W-IEMRs) offer significant advantages in stability and hydrodynamic performance but have limited catalytic efficiency due to long diffusion paths in the microreactor. This study used a computational fluid dynamics (CFD) model to simulate flow fields and concentration distributions in serpentine micro-channels, quantifying mixing efficiency via the mixing index (MI) and Dean number (De). By testing channels with different curvature radius, bending ratios, and inner diameters, the geometry was optimized to enhance mass transfer and mixing. Simulations showed that a smaller curvature radius, larger bending ratio, and smaller inner diameter strengthen Dean vortices, improving mixing. However, catalytic efficiency has a non-monotonic relationship with these parameters: a curvature radius below 6.31 mm reduces enzyme-substrate contact due to excessive vortices, while a bending ratio exceeding 60 % leads to uneven substrate distribution caused by counter-rotating vortices in adjacent sections, impairing performance. Optimal design parameters are: curvature radius 6.31 mm, bending ratio 60 %, inner diameter 0.5 mm. For micro-channels with varying reaction kinetics, careful consideration must be given to balancing mass transfer and catalytic performance.

微流体技术在酶生物技术中有着广泛的应用。壁涂固定化酶微通道（W-IEMRs）在稳定性和流体动力学性能方面具有显著的优势，但由于微反应器中的扩散路径较长，限制了催化效率。本研究采用计算流体动力学（CFD）模型模拟蛇形微通道内的流场和浓度分布，通过混合指数（MI）和迪安数（De）量化混合效率。通过测试不同曲率半径、弯曲比和内径的通道，优化了通道的几何形状，以增强传质和混合。模拟结果表明，较小的曲率半径、较大的弯曲比和较小的内径增强了Dean涡，改善了混合。然而，催化效率与这些参数之间存在非单调关系：曲率半径低于6.31 mm会由于涡流过大而减少酶与底物的接触，而弯曲比超过60 %则会由于相邻截面的反旋转涡流而导致底物分布不均匀，从而影响催化效率。优化设计参数为：曲率半径6.31 mm，弯曲比60 %，内径0.5 mm。对于反应动力学变化的微通道，必须仔细考虑传质和催化性能的平衡。

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