Biochemical Engineering Journal最新文献_第4页

Advanced bioelectronic scaffolds with electrostimulation for enhanced muscle regeneration 先进的生物电子支架与电刺激增强肌肉再生

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-02-02 DOI: 10.1016/j.bej.2026.110105

Xi Yang , Jianmin Wang , Liang Zhang , Fengling Zhuo , Linyan Ge , Liuhang Zhang , Jie Li , Fei Han , Guanghui Song , Xiaozhi Wang

Tissue engineering scaffolds are essential for facilitating tissue regeneration, and electrical stimulation has emerged as a powerful complementary strategy to accelerate this process. However, the functionality and performance of current scaffold systems remain suboptimal, limiting their therapeutic potential. In this study, a novel graphene-boron nitride-poly(lactic-co-glycolic acid) (GR-BN-PLGA) scaffold was fabricated using 3D printing technology for the repair of abdominal wall hernias in rats. The scaffold was constructed using two materials with distinct electrical properties, conductive GR-PLGA and insulating BN-PLGA, through a layered printing process. By integrating electrodes and microneedles, the scaffold was designed to establish a centrally directed electric field in the abdominal defect area, enabling effective electrical stimulation therapy. The results demonstrated that the combined application of the scaffold and electrical stimulation significantly upregulated the expression of α-smooth muscle actin, type I collagen, and Cell Proliferation Marker Protein Ki-67, thereby facilitating tissue remodeling. Meanwhile, the lower expression levels of proliferating cell nuclear antigen and connective tissue growth factor effectively suppressed excessive proliferation and fibrosis, aiding in the formation of stable and functional regenerated tissue. The synergistic application of conductive scaffolds and electrical stimulation provides a novel strategy for tissue repair and highlights its tremendous potential in accelerating tissue regeneration and promoting the formation of functional tissues.

组织工程支架对于促进组织再生至关重要，电刺激已成为加速这一过程的有力补充策略。然而，目前支架系统的功能和性能仍然不够理想，限制了它们的治疗潜力。本研究采用3D打印技术制备了新型石墨烯-氮化硼-聚乳酸-羟基乙酸（GR-BN-PLGA）支架，用于大鼠腹壁疝修复。支架采用导电GR-PLGA和绝缘BN-PLGA两种具有不同电性能的材料，通过分层印刷工艺构建而成。通过整合电极和微针，该支架被设计用于在腹部缺陷区域建立一个中心定向电场，从而实现有效的电刺激治疗。结果表明，支架与电刺激联合应用可显著上调α-平滑肌肌动蛋白、I型胶原和细胞增殖标记蛋白Ki-67的表达，促进组织重塑。同时，增殖细胞核抗原和结缔组织生长因子的低表达水平有效抑制了过度增殖和纤维化，有助于形成稳定和功能的再生组织。导电支架和电刺激的协同应用为组织修复提供了一种新的策略，并突出了其在加速组织再生和促进功能组织形成方面的巨大潜力。

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引用次数: 0

Enhanced anticancer activity through bidirectional fermentation of Epimedium brevicornum Maxim. with Morchella esculenta 短角淫羊藿双向发酵增强抗癌活性。羊肚菌

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-01-30 DOI: 10.1016/j.bej.2026.110103

Chengchuan Che , Lijun Sheng , Pingping Ding , Qi Liu , Huimin Zhao , Wenyu Han , Yunli Guo , Cuijuan Gao , Carol Sze Ki Lin , Xinxin Liang

Epimedium brevicornum Maxim. (EB), a traditional Chinese herbal medicine, exhibits notable anticancer and anti-aging activities attributed primarily to flavonoids such as epimedin C and icariin. However, its effective application is limited by low extraction efficiency and incomplete mechanistic understanding. Bidirectional fermentation is an effective microbial bioconversion strategy to enrich bioactive constituents or generate novel metabolites. Herein, the medicinal fungus Morchella esculenta (ME) was used to conduct bidirectional fermentation of EB. Compared with single-fermentation EB extract, the ME/EB extract had 2.39-fold and 1.44-fold greater epimedin C and icariin content, respectively. The ME/EB extract exhibited significant antiproliferative activity against cancer cells, with IC₅₀ values of 215 μg/mL for human cervical cancer HeLa cells and 235 μg/mL for non-small-cell lung cancer A549 cells. Moreover, the ME/EB extract induced cell-cycle arrest at G1/G0 phase in HeLa cells and S phase in A549 cells, while markedly suppressing cell migration and invasion. The ME/EB extract also down-regulated Bcl-2 protein expression and up-regulated Bax, PI3K, and Akt protein expression, thereby promoting cancer cell apoptosis. Therefore, bidirectional fermentation significantly enhanced EB anticancer activity, potentially through modulation of the PI3K/Akt signalling pathway. Overall, ME-mediated bidirectional fermentation of EB shows promise as a novel strategy for developing anticancer agents.

短角淫羊藿。中药叶黄素（EB）具有显著的抗肿瘤和抗衰老活性，其主要成分是淫羊藿苷和淫羊藿苷等黄酮类化合物。但萃取效率低，对其机理认识不全，限制了其有效应用。双向发酵是一种有效的微生物生物转化策略，可以丰富生物活性成分或产生新的代谢产物。本研究利用药用真菌羊肚菌（Morchella esculenta， ME）对EB进行双向发酵。与单发酵EB提取物相比，ME/EB提取物的淫羊藿苷C和淫羊藿苷含量分别提高了2.39倍和1.44倍。ME/EB提取物对癌细胞具有显著的抗增殖活性，对人宫颈癌HeLa细胞的IC₅0值为215 μg/mL，对非小细胞肺癌A549细胞的IC₅0值为235 μg/mL。此外，ME/EB提取物诱导HeLa细胞的G1/G0期和A549细胞的S期细胞周期停滞，同时显著抑制细胞迁移和侵袭。ME/EB提取物还能下调Bcl-2蛋白表达，上调Bax、PI3K和Akt蛋白表达，从而促进癌细胞凋亡。因此，双向发酵显著增强EB的抗癌活性，可能是通过调节PI3K/Akt信号通路。综上所述，me介导的EB双向发酵有望成为开发抗癌药物的新策略。

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引用次数: 0

Innovatively engineered biowaste-derived CaO–Fe/activated carbon and ce/sepiolite composite catalysts for microwave-intensified biodiesel production from waste cooking oil 创新设计的生物垃圾衍生的CaO-Fe /活性炭和ce/海泡石复合催化剂，用于从废食用油中微波强化生产生物柴油

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-01-26 DOI: 10.1016/j.bej.2026.110097

Hiba A. Abdulkareem , Mohammed T. Yassen , Nooralhuda N. Ahmed , Malak. B.Ahmed , Saba A. Gheni , Mudheher M. Ali , Farah T. Al-Sudani , Ataallah K. Tahah

The development of low-cost and sustainable catalytic systems is essential for advancing biodiesel production from waste lipids. This study presents two novel heterogeneous catalysts derived entirely from natural and biowaste resources: (i) CaO–Fe supported on activated carbon produced from eggshells and date palm fronds (CaO–Fe/AC), and (ii) cerium-modified activated sepiolite (Ce/AS). The catalysts were synthesized via incipient wetness impregnation followed by thermal activation and comprehensively characterized using BET, SEM, XRD, FTIR, TGA, and ICP-OES analyses. The CaO–Fe/AC catalyst exhibited strong basicity and high surface area favorable for transesterification, while the Ce/AS catalyst provided bifunctional acid–base properties enabling the direct conversion of high–free fatty acid (FFA) waste cooking oil (WCO) through simultaneous esterification and transesterification. Process intensification using microwave irradiation significantly reduced reaction time and energy consumption. Under optimized conditions (methanol-to-oil ratio of 9:1, catalyst loading of 0.3 wt%, and 100 % microwave power), biodiesel conversions of 97 % for CaO–Fe/AC and 99 % for Ce/AS were achieved within 6 min. The produced biodiesel met ASTM D6751 and EN 14214 specifications. The combination of microwave-assisted heating and biowaste-derived composite catalysts offers a rapid, energy-efficient, and environmentally sustainable route for biodiesel production, supporting circular economy strategies and scalable renewable fuel technologies.

开发低成本和可持续的催化系统是推进废脂生物柴油生产的关键。本研究提出了两种完全来自自然和生物废弃物资源的新型多相催化剂：(i)蛋壳和椰枣叶活性炭负载的CaO-Fe （CaO-Fe /AC）和（ii）铈修饰的活化海泡石（Ce/AS）。采用初湿浸渍-热活化法制备催化剂，并用BET、SEM、XRD、FTIR、TGA、ICP-OES等方法对催化剂进行了综合表征。CaO-Fe /AC催化剂具有强碱性和高表面积，有利于酯交换；Ce/AS催化剂具有酸碱双功能，可通过同时酯化和酯交换直接转化高游离脂肪酸废食用油（WCO）。微波辐照强化工艺显著缩短了反应时间和能耗。在优化条件下（甲醇油比为9:1，催化剂负载为0.3 wt%，微波功率为100% %），在6 min内，CaO-Fe /AC和Ce/AS的生物柴油转化率分别达到97 %和99 %。生产的生物柴油符合ASTM D6751和EN 14214规格。微波辅助加热和生物废物衍生的复合催化剂的结合为生物柴油的生产提供了一条快速、节能和环境可持续的途径，支持循环经济战略和可扩展的可再生燃料技术。

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引用次数: 0

Genetically encoded FRET-based sensor for intracellular heparin monitoring in real time 基因编码的基于fret的细胞内肝素实时监测传感器

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-01-27 DOI: 10.1016/j.bej.2026.110099

Sana Masroor, Neha Soleja, Mohamad Aman Jairajpuri, Mohd Mohsin

Heparin is an indispensable anticoagulant commonly administered during surgical interventions that require extracorporeal blood circulation, including cardiopulmonary bypass surgery. However, heparin overdose frequently leads to potentially fatal bleeding complications. Hence, precise monitoring of heparin levels is critical to avoid risks such as heparin-induced thrombocytopenia (HIT) and hemorrhage. Recently, Various detection tools have been designed for heparin, but these have complex procedures, low sensitivity, and do not perform real-time monitoring of heparin. Therefore, we have designed a highly effective genetically encoded fluorescence resonance energy transfer (FRET)-based Heparin Sensor (HepSen) to perform real-time monitoring of heparin with high spatio-temporal resolution. HepSen was developed by incorporating heparin-binding protein, platelet factor 4 (PF4) protein, between ECFP and Venus at N- and C-terminus, respectively. This novel nanosensor exhibits high stability at physiological pH levels and remains unaffected by the addition of biologically significant metal ions. Furthermore, two mutant variants, C12G and R20A, were also designed; nevertheless, wild-type HepSen showed the highest binding affinity with K_d of 2.73 × 10⁻⁷ M and a broad detection range of 1 nM-50 µM. HepSen exhibited robust expression in Escherichia coli (E. coli), Saccharomyces cerevisiae (S. cerevisiae), and human embryonic kidney (HEK-293T) cells, facilitating real-time monitoring of heparin with a well-defined saturation curve, thereby demonstrating its intracellular sensing capability across diverse biological systems.

肝素是一种不可缺少的抗凝剂，通常在需要体外血液循环的外科手术中使用，包括体外循环手术。然而，肝素过量经常导致潜在的致命出血并发症。因此，精确监测肝素水平对于避免肝素诱发的血小板减少症（HIT）和出血等风险至关重要。近年来，人们设计了多种肝素检测工具，但这些工具程序复杂，灵敏度低，不能实时监测肝素。因此，我们设计了一种高效的基于基因编码荧光共振能量转移（FRET）的肝素传感器（HepSen），以实现高时空分辨率的肝素实时监测。HepSen是通过将肝素结合蛋白、血小板因子4 （PF4）蛋白分别置于ECFP和Venus的N端和c端而开发的。这种新型纳米传感器在生理pH水平下具有很高的稳定性，并且不受添加具有生物意义的金属离子的影响。此外，还设计了C12G和R20A两个突变体；然而，野生型HepSen的结合亲和力最高，Kd为2.73 × 10−7 M，检测范围为1 nM-50µM。HepSen在大肠杆菌（E. coli）、酿酒酵母（S. cerevisiae）和人胚胎肾（HEK-293T）细胞中表现出强大的表达，通过定义明确的饱和曲线促进对肝素的实时监测，从而证明了其在不同生物系统中的细胞内传感能力。

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引用次数: 0

Enhanced expression and purification strategy for β2-microglobulin in Escherichia coli β2微球蛋白在大肠杆菌中的增强表达及纯化策略

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-01-30 DOI: 10.1016/j.bej.2026.110100

Boram Kim , Junwoo Hwang , Dong-Hyun Seo , Sun Taek Kim , Ji-Hun Kim , Kyoung-Seok Ryu

β2-microglobulin (β2m) is a biomarker for various renal diseases and forms the major histocompatibility complex class I (MHC-I) with the heavy chain. The presenting antigenic peptide on the MHC-I is critical for the cytotoxic T-cell mediated immune responses, and thus extensive efforts have been paid to the in vitro reconstitution of the MHC-I through co-refolding of the heavy chain and β2m. Although β2m that contains a single disulfide bond is highly stable, it is typically expressed as an insoluble form in Escherichia coli (E. coli). In this study, native β2m was prepared via soluble expression in the E. coli SHuffle T7 Express strain, achieving a yield of 30–40 mg per liter of Luria-Bertani (LB) medium, more than 10-fold higher than that obtained through periplasmic expression. The refolding of the heavy chain with the purified β2m successfully produced the cognate peptide-loaded MHC-I (pMHC-I). The SHuffle T7 system offers a straightforward approach for producing functional β2m for future researches in immunology and structural biology.

β2-微球蛋白（β2m）是多种肾脏疾病的生物标志物，与重链形成主要的组织相容性复合体I类（MHC-I）。MHC-I上的递呈抗原肽对于细胞毒性t细胞介导的免疫应答至关重要，因此通过重链和β2m的共重折叠来体外重建MHC-I已经付出了大量的努力。虽然含有单键二硫键的β2m高度稳定，但它在大肠杆菌（E. coli）中通常以不溶形式表达。本研究采用可溶性表达法在大肠杆菌SHuffle T7 Express菌株中制备了天然β2m，在LB培养基中获得了30-40 mg / l的产量，比采用周质表达法获得的产量高出10倍以上。重链与纯化的β2m重折叠成功地产生同源肽装载MHC-I （pMHC-I）。SHuffle T7系统为免疫学和结构生物学的未来研究提供了一种直接产生功能性β2m的方法。

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引用次数: 0

A combination of domain fusion and homologous site transplantation improves the performance of Bst DNA polymerase in denaturation bubble-mediated strand exchange amplification 结构域融合和同源位点移植的结合提高了Bst DNA聚合酶在变性气泡介导的链交换扩增中的性能

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-01-30 DOI: 10.1016/j.bej.2026.110104

Quanling Dong , Qiming Chen , Keren Shang , Zhengrong Lu , Yuanlong Hu , Zhanmin Liu

Bst DNA polymerase has been widely used in isothermal nucleic-acid amplification platforms for pathogen detection and molecular diagnostics. Improving the catalytic efficiency and operational robustness of Bst DNA polymerase through protein engineering is therefore of substantial interest. In this study, we combined domain fusion with the introduction of a homologous site transplantation in the large fragment of Bst DNA polymerase (BstLF). Specifically, the double-stranded DNA-binding domain Sso7d was fused to BstLF via a flexible linker, while the F496H mutation was introduced. The resulting mutant, Sso7d-BstLF(F496H), demonstrated improved catalytic efficiency in isothermal amplification techniques of denaturation bubble-mediated strand exchange amplification (SEA). Compared with the wild-type, Sso7d-BstLF(F496H) reduced the SEA amplification time by approximately 50 %. In addition, the engineered polymerase retained robust amplification activity at 71°C and showed improved tolerance to pH fluctuations and to common inhibitory components (NaCl, EDTA, urea, ethanol, and SDS). Molecular docking and mutation energy calculations suggested that improved performance might be associated with additional hydrogen bonds between the R group and DNA, consistent with increased protein-DNA affinity. Molecular dynamics simulations further indicated that F496H preserves global structural stability while reducing local flexibility, providing a plausible structural basis for the observed activity enhancement. Collectively, these findings identify Sso7d–BstLF(F496H) as a promising polymerase for improved isothermal amplification–based molecular diagnostics.

Bst DNA聚合酶已广泛应用于病原体检测和分子诊断的等温核酸扩增平台。因此，通过蛋白质工程提高Bst DNA聚合酶的催化效率和操作稳健性具有重要意义。在这项研究中，我们将结构域融合与在Bst DNA聚合酶（BstLF）大片段中引入同源位点移植相结合。具体来说，双链dna结合域Sso7d通过一个柔性连接体融合到BstLF中，同时引入F496H突变。由此产生的突变体Sso7d-BstLF（F496H）在变性气泡介导链交换扩增（SEA）的等温扩增技术中表现出更高的催化效率。与野生型相比，Sso7d-BstLF（F496H）将SEA扩增时间缩短了约50% %。此外，工程聚合酶在71°C下保持了强大的扩增活性，并表现出对pH波动和常见抑制成分（NaCl， EDTA，尿素，乙醇和SDS）的耐受性增强。分子对接和突变能量计算表明，性能的提高可能与R基团和DNA之间额外的氢键有关，这与蛋白质-DNA亲和力的增加相一致。分子动力学模拟进一步表明，F496H保持了整体结构稳定性，同时降低了局部柔韧性，为观察到的活性增强提供了合理的结构基础。总的来说，这些发现确定Sso7d-BstLF （F496H）是一种有前途的聚合酶，用于改进等温扩增的分子诊断。

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引用次数: 0

Nitrogen removal performance and low-temperature impact of hybrid membrane aerated biofilms reactor (H-MABR) 混合膜曝气生物膜反应器（H-MABR）脱氮性能及低温影响

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-01-29 DOI: 10.1016/j.bej.2026.110102

Youzhao Wang , Jie Han , Mingdong Chang , Yongguang Ma , Xiaoyan Dang , Shumin He , Zhipeng Wang , Rongxiao Zhang , Junnan Liu , Jinxiang Wang , Lin Zhai , Junting Wang , Zhenning Lv , Tong Zhu

Declining microbial activity under low-temperature conditions poses a persistent challenge for biological wastewater treatment. Conventional Membrane Aerated Biofilm Reactor (C-MABR) has the advantages of high mass transfer efficiency, stable treatment effect and low aeration energy consumption. However, treatment capacity of C-MABR is significantly compromised under low-temperature conditions. In this work, a novel hybrid membrane aerated biofilm reactor (H-MABR) was developed by integrating biomass carriers with membrane aeration to enhance system robustness in cold conditions. Performance, process optimization, and microbial community characteristics of H‑MABR were systematically evaluated at

10 \sim 12 ℃

. The results indicated that under approximately 200 mg/L of COD, approximately 50 mg/L of

{NH}_{4}^{+} - N

and 12 h of HRT, COD removal loading rates averaged 0.244 kg/(m³∙d),

{NH}_{4}^{+} - N

removal loading rates averaged 0.051 kg N/(m³∙d) and TN removal loading rates averaged 0.038 kg N/(m³∙d), respectively. Using response surface methodology (RSM), optimal operating window for pollutant removal was identified as followed: temperature

10 \sim 12 ℃

, pH 7.5, C/N ratio 3.71, and aeration pressure 0.02 MPa. Microbial community analysis demonstrated that exposure to low temperature markedly reshaped the community structure in H‑MABR, indicating a strong linkage between temperature and functional microbial community. Overall, this study elucidated impact of low-temperature operation on H‑MABR performance and microbial ecology and provided practical insights to guide the design and operation for wastewater treatment in cold environments.

低温条件下微生物活性下降是废水生物处理的一个长期挑战。常规膜曝气生物膜反应器（C-MABR）具有传质效率高、处理效果稳定、曝气能耗低等优点。然而，在低温条件下，C-MABR的处理能力明显受损。在这项工作中，通过将生物质载体与膜曝气相结合，开发了一种新型混合膜曝气生物膜反应器（H-MABR），以提高系统在寒冷条件下的稳健性。在10 ~ 12℃条件下对H‑MABR的性能、工艺优化和微生物群落特征进行了系统评价。结果表明，在约200 mg/L COD、约50 mg/L NH4+−N和12 h HRT条件下，COD去除率平均为0.244 kg/(m³∙d)， NH4+−N去除率平均为0.051 kg N/(m³∙d)， TN去除率平均为0.038 kg N/（m³∙d）。利用响应面法（RSM）确定了污染物去除的最佳操作窗口：温度10 ~ 12℃，pH值7.5，C/N比3.71，曝气压力0.02 MPa。微生物群落分析表明，低温显著重塑了H - MABR的群落结构，表明温度与功能微生物群落之间存在很强的联系。总体而言，本研究阐明了低温运行对H - MABR性能和微生物生态的影响，为指导低温环境下废水处理的设计和运行提供了实践见解。

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引用次数: 0

A novel multi-directional modular mixing system for bringing new insight to microalgae production in panel photobioreactors 一种新型的多向模块化混合系统，为面板光生物反应器中的微藻生产带来了新的见解

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-04-01 Epub Date: 2026-01-08 DOI: 10.1016/j.bej.2026.110074

Barış Erdoğan , S. Furkan Demi̇rden , Deniz Senyay-Oncel , Suphi S. Oncel

A novel system was designed herein to enhance the mixing efficiency of conventional panel photobioreactors (PBRs). Initially, design optimization was conducted using three-dimensional computational modeling. For this, CFD simulations performed using the Shear Stress Transport model of different mixing configurations. These are single vertical shaft (1B), single turbine on each lateral shafts (2B) and single turbines in all directions (3B), respectively. Rushton and marine type impellers are used in these simulations for comprehensive evaluation. Here, Chlamydomonas reinhardtii CC-124 was used as model microalgae. In both simulations and validation studies Rushton turbine gives better results (36.81 ± 0.23 mg.L⁻¹) when compared with marine impeller (16.42 ± 0.67 mg.L⁻¹) in terms of highest total chlorophyll amount reached. Among the different configurations with Rushton impeller, the 1B and 3B configurations come forward. Although, 3B reached higher average shear stress value (3.63 Pa) than 1B, this configuration was able to reach higher microalgae concentration in a short time during the 13-day culture period when evaluated in terms of biomass. This result indicates that 3B configuration which creates highest magnitude multidirectional flow vectors provides a consistent homogeneous mixing for better biomass production in PBR. Based on these results, it can be said that this modular mixing system design is a promising contribution for panel PBRs and new microalgae production systems.

为了提高面板型光生物反应器（PBRs）的混合效率，设计了一种新型系统。最初，采用三维计算建模进行设计优化。为此，采用不同混合配置的剪切应力输运模型进行CFD模拟。它们分别是单垂直轴（1B），每侧轴上的单涡轮（2B）和所有方向上的单涡轮（3B）。在这些模拟中使用了Rushton和marine型叶轮进行综合评价。本文以莱茵衣藻CC-124为模型微藻。在模拟和验证研究中，Rushton涡轮的最高叶绿素含量（36.81 ± 0.23 mg.L−1）优于船用叶轮（16.42 ± 0.67 mg.L−1）。在Rushton叶轮的不同配置中，提出了1B和3B两种配置。虽然3B的平均剪应力值（3.63 Pa）高于1B，但从生物量的角度来看，在13天的培养期内，这种配置能够在短时间内达到更高的微藻浓度。该结果表明，产生最大量级多向流矢量的3B配置为PBR中更好的生物质生产提供了一致的均匀混合。基于这些结果，可以说这种模块化混合系统设计对板式pbr和新的微藻生产系统有很大的贡献。

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引用次数: 0

Configuration and optimization of in-situ microbial protein production via bio-electrochemical system 生物电化学原位微生物蛋白生产系统的配置与优化

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-04-01 Epub Date: 2026-01-12 DOI: 10.1016/j.bej.2026.110081

Yilin Jiang , Hangyu Sun , Shimin Wu , Xiangjie Xiao , Yanping Liu , Ziyi Yang

Upcycling of nitrogen for producing microbial protein (MP) is a promising method to support the development of circular economy. In this study, in-situ MP production system was upgraded based on microbial electrolytic cell (MEC). Firstly, ammonia-tolerant electroactive microorganisms were enriched on the bio-anode electrode at 1500 mg N/L. Secondly, to enhance the NH₄⁺ -N migration efficiency, ion concentration in the cathode chamber of 20 mmol K₂HPO₄ + 20 mmol Na₂HPO₄, pH in the cathode chamber of 7.0 and applied voltage of 0.6 V was selected. Thirdly, in-situ MP production system showed the feasibility, with MP production of 1.82 g/L. Finally, voltage of 1.0 V, NH₄⁺-N concentration of 1 g N/L in anode chamber and 20°C were selected as the optimal operational conditions. And MP production of 2.19 g/L was obtained, with 30.47 % of nitrogen stored in MP. Essential amino acids of MP produced in this study was about 42 %, belonging to the high-quality proteins. In-situ protein production system showed the promising MP synthesis capacity, which could be used as a sustainable solution for nitrogen upcycling.

氮的升级回收生产微生物蛋白是支持循环经济发展的一种很有前途的方法。本研究对基于微生物电解池（MEC）的原位MP生产系统进行了升级。首先，在1500 mg N/L的生物阳极电极上富集耐氨电活性微生物。其次，为提高NH4+ -N迁移效率，阴极室离子浓度为20 mmol K2HPO4 + 20 mmol Na2HPO4，阴极室pH为7.0，施加电压为0.6 V。第三，原位生产MP系统显示出可行性，MP产量为1.82 g/L。最终选择电压为1.0 V，阳极室NH4+-N浓度为1 g N/L，温度为20℃为最佳操作条件。MP的产量为2.19 g/L，其中30.47 %的氮储存在MP中。本研究生产的MP必需氨基酸含量约为42% %，属于优质蛋白质。原位蛋白质生产系统显示出良好的MP合成能力，可作为氮升级循环的可持续解决方案。

{"title":"Configuration and optimization of in-situ microbial protein production via bio-electrochemical system","authors":"Yilin Jiang , Hangyu Sun , Shimin Wu , Xiangjie Xiao , Yanping Liu , Ziyi Yang","doi":"10.1016/j.bej.2026.110081","DOIUrl":"10.1016/j.bej.2026.110081","url":null,"abstract":"<div><div>Upcycling of nitrogen for producing microbial protein (MP) is a promising method to support the development of circular economy. In this study, <em>in-situ</em> MP production system was upgraded based on microbial electrolytic cell (MEC). Firstly, ammonia-tolerant electroactive microorganisms were enriched on the bio-anode electrode at 1500 mg N/L. Secondly, to enhance the NH<sub>4</sub><sup>+</sup> -N migration efficiency, ion concentration in the cathode chamber of 20 mmol K<sub>2</sub>HPO<sub>4</sub> + 20 mmol Na<sub>2</sub>HPO<sub>4</sub>, pH in the cathode chamber of 7.0 and applied voltage of 0.6 V was selected. Thirdly, <em>in-situ</em> MP production system showed the feasibility, with MP production of 1.82 g/L. Finally, voltage of 1.0 V, NH<sub>4</sub><sup>+</sup>-N concentration of 1 g N/L in anode chamber and 20°C were selected as the optimal operational conditions. And MP production of 2.19 g/L was obtained, with 30.47 % of nitrogen stored in MP. Essential amino acids of MP produced in this study was about 42 %, belonging to the high-quality proteins. <em>In-situ</em> protein production system showed the promising MP synthesis capacity, which could be used as a sustainable solution for nitrogen upcycling.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"228 ","pages":"Article 110081"},"PeriodicalIF":3.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZrO2/g-C3N4 heterojunction nanocomposites for enhanced photocatalytic degradation of organic pollutants and antibacterial activity ZrO2/g-C3N4异质结纳米复合材料增强光催化降解有机污染物和抗菌活性

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-04-01 Epub Date: 2026-01-19 DOI: 10.1016/j.bej.2026.110085

Shweta Vashisth, S.P. Nehra

In the current study, zirconium oxide/graphitic carbon nitride (ZrO₂/g-C₃N₄) nanocomposites have been synthesized using Murraya koenigii leaf extract as an eco-friendly reducing and stabilizing agent. The properties of the ZrO₂/g-C₃N₄ nanocomposites were investigated by employing several analytical techniques such as X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), high-resolution transmission electron microscopy (HRTEM), UV-Vis diffuse reflectance spectroscopy (UV-DRS), field emission scanning electron microscopy (FESEM), energy-dispersive X-ray analysis (EDX), and elemental mapping. The photocatalytic activity of the nanocomposites was evaluated through the use of ultraviolet-visible (UV-Vis) spectroscopy for the removal of the organic dyes, xylenol orange (XO) and malachite green (MG) dyes. Following a thorough analysis, it has been demonstrated that, in comparison to other samples, the ZrCN 2 nanocomposite showed significantly enhanced photocatalytic dye degradation activity for malachite green (94.31 %) and xylenol orange (59.28 %) dyes. To support the potential use of the synthesized nanomaterials against Escherichia coli, an antibacterial study has also been conducted. ZrCN 2 showed the highest antibacterial activity against E. coli compared to the other samples, with an 8-mm zone of inhibition and the lowest minimum inhibitory concentration (30 µg). The composite's recyclability test has been performed for up to five cycles, and its photodegradation results remained consistent. Moreover, a scavenger experiment was conducted to determine the major reactive oxygen species (ROS) involved in the photocatalytic mechanism. The outcomes of the present investigation offer important insights into the synthesis and characterization of ZrO₂/g-C₃N₄ nanocomposites that demonstrate enhanced photocatalytic dye degradation and antibacterial efficiency as compared to pure g-C₃N₄ and ZrO₂ nanoparticles.

本研究以木树叶提取物为还原剂和稳定剂，合成了氧化锆/石墨氮化碳（ZrO2/g-C3N4）纳米复合材料。采用x射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、热重分析（TGA）、x射线光电子能谱（XPS）、高分辨率透射电子显微镜（HRTEM）、紫外-可见漫反射光谱（UV-DRS）、场发射扫描电子显微镜（FESEM）、能量色散x射线分析（EDX）和元素映射等分析技术研究了ZrO2/g-C3N4纳米复合材料的性能。利用紫外-可见（UV-Vis）光谱法评价了纳米复合材料对有机染料、二甲酚橙（XO）和孔雀石绿（MG）染料的光催化活性。经过深入的分析，与其他样品相比，zrcn2纳米复合材料对孔雀石绿（94.31 %）和二甲酚橙（59.28 %）染料的光催化降解活性显著增强。为了支持合成的纳米材料对抗大肠杆菌的潜在用途，还进行了一项抗菌研究。ZrCN 2对大肠杆菌的抑菌活性最高，抑菌带为8 mm，最低抑菌浓度为30 µg。复合材料的可回收性测试已经进行了多达五个循环，其光降解结果保持一致。此外，还进行了清除剂实验，以确定参与光催化机制的主要活性氧（ROS）。本研究的结果为ZrO2/g-C3N4纳米复合材料的合成和表征提供了重要的见解，与纯g-C3N4和ZrO2纳米颗粒相比，它们具有更好的光催化染料降解和抗菌效率。

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引用次数: 0