Biochemical Engineering Journal最新文献_第4页

Enhanced microalgae cultivation and simultaneous achievement of nutrient removal from actual anaerobic digestion effluent of a full-scale kitchen waste plant 增强微藻培养，同时实现从实际厌氧消化污水中去除营养物

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-08 DOI: 10.1016/j.bej.2025.110043

Wenwei Zhang , Yi Zhang , Meixing Xiao , Sijin Wu , Jialu Liu , Lie Yang

Conventional microalgae cultivation media are cost-prohibitive, necessitating alternative nutrient sources to enable economically viable biomass production for value-added applications. This study investigated the cultivation of Chlorella and Scenedesmus using actual anaerobic digestion effluent (ADE) from untreated kitchen waste. Despite an unfavorable C:N:P ratio, indigenous bacteria and other potential stressors, ADE culture conditions promoted superior growth rates and biomass accumulation in Chlorella. Aeration further increased biomass accumulation, with Chlorella achieving significantly higher yields than Scenedesmus. Specifically, Chlorella attained a biomass yield of 0.537 g/L and a growth rate of 0.218 μ/d. This enhanced performance is attributed to Chlorella's greater ammonia tolerance and efficient Ca²⁺ uptake (≤99.21 %). Remarkably, aeration increased Chlorella's extracellular polymeric substance (EPS) content by 88.56 %. And this system achieved simultaneous nutrient removal (37 % COD, 87 % TP) and biomass production, with potential for enhanced pollutant removal efficiency at reduced hydraulic retention times (HRT = 4–6 days). Fluorescence analysis revealed dissolved organic matter (DOM) supported microalgae growth through dual mechanisms: providing a carbon supply and facilitating heavy metal detoxification. These findings indicated that optimizing the pretreatment process of anaerobic digestate to achieve the transformation of complex organic matter in it may further enhance its potential for deep treatment of anaerobic digestate and microalgae culture.

传统的微藻培养基成本过高，需要替代营养来源，以实现经济上可行的生物质生产，用于增值应用。本研究利用未经处理的厨余垃圾实际厌氧消化出水（ADE）培养小球藻和场景藻。尽管C:N:P比、本地细菌和其他潜在胁迫因素不利，ADE培养条件促进了小球藻的生长速度和生物量积累。曝气进一步增加了生物量积累，其中小球藻的产量显著高于情景藻。其中，小球藻的生物量为0.537 g/L，生长速率为0.218 μ/d。这种增强的性能归因于小球藻更强的氨耐受性和高效的Ca 2 +吸收（≤99.21 %）。曝气显著提高了小球藻胞外聚合物（EPS）含量88.56 %。该系统同时实现了营养物去除（37% % COD, 87% % TP）和生物质生产，并且在减少水力停留时间（HRT = 4-6天）的情况下，具有提高污染物去除效率的潜力。荧光分析显示，溶解有机物（DOM）通过提供碳供应和促进重金属解毒的双重机制支持微藻生长。上述结果表明，优化厌氧消化液的预处理工艺，实现厌氧消化液中复杂有机物的转化，可以进一步增强厌氧消化液深度处理和微藻培养的潜力。

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引用次数: 0

Ready-to-use cryopreservation of undifferentiated induced pluripotent stem cells (iPSCs) without detachment from culture plates using D-proline and a synthetic polymer 使用d -脯氨酸和合成聚合物冷冻保存未分化的诱导多能干细胞（iPSCs），使其不脱离培养板

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-06 DOI: 10.1016/j.bej.2025.110041

Kenta Morita , Shinya Kawasaki , Tomoko Yashiro , Ryoko Futai , Chanhyon Kin , Aito Nakahashi , Hikaru Amo , Yukiya Kitayama , Takashi Aoi , Michiyo Koyanagi-Aoi , Tatsuo Maruyama

Induced pluripotent stem cells (iPSCs) have been making a significant impact on the fields of regenerative medicine and cell biology. Several cell-freezing media for dispersed single-cell iPSCs are already commercially available. However, cryopreservation techniques for iPSCs cultured in 2D and 3D formats remain to be established. In this study, we developed a xeno-free cell-freezing medium containing D-proline and a synthetic block copolymer composed of 2-(dimethylamino)ethyl methacrylate (DEGMA) and 2-methacryloyloxyethyl phosphorylcholine (MPC), designated PDEGMA-b-PMPC-b-PDEGMA, that enables the cryopreservation of iPSCs cultured in 2D on microplates without detachment of the cells. Prior to cryopreservation, 2D-cultured iPSCs were treated with TrypLE Select Enzyme to weaken their adhesion to the microplate surfaces. Subsequently, the cells were cryopreserved in the cell-freezing medium containing D-proline and PDEGMA-b-PMPC-b-PDEGMA at −80°C for 2 days. At 48 h after thawing, the cell recovery (cell viability) was at least 70 % relative to the cell viability before freezing, while the cell recovery with commercially available media was 1.2 % at most. The most effective composition of the cell-freezing medium was 10 vol% DMSO, 5 vol% Dulbecco’s modified Eagle’s medium, 85 vol% water, 1 % (w/v) PDEGMA-b-PMPC-b-PDEGMA, 2 % (w/v) D-proline, and 0.35 % (w/v) NaCl. The cell recovery value remained stable after 3 months of cryopreservation. Most importantly, the iPSCs maintained their pluripotency after cryopreservation in the newly developed cell-freezing medium.

诱导多能干细胞（iPSCs）在再生医学和细胞生物学领域产生了重大影响。用于分散的单细胞iPSCs的几种细胞冷冻介质已经在商业上可用。然而，以2D和3D格式培养的iPSCs的冷冻保存技术仍有待建立。在这项研究中，我们开发了一种含有d -脯氨酸和由2-（二甲氨基）甲基丙烯酸乙酯（DEGMA）和2-甲基丙烯酰氧乙基磷酸胆碱（MPC）组成的合成嵌段共聚物（命名为PDEGMA-b-PMPC-b-PDEGMA）的无xeno细胞冷冻培养基，该培养基可以在微孔板上低温保存2D培养的iPSCs，而不会使细胞脱落。在冷冻保存之前，用TrypLE Select酶处理2d培养的iPSCs以削弱其与微孔板表面的粘附。随后，细胞在含有d -脯氨酸和PDEGMA-b-PMPC-b-PDEGMA的细胞冷冻培养基中低温保存2天，温度为- 80℃。解冻后48 h，细胞回收率（细胞活力）相对于冷冻前的细胞活力至少为70 %，而使用市售培养基的细胞回收率最多为1.2 %。最有效的细胞冷冻培养基组成为：10 vol% DMSO， 5 vol% Dulbecco 's modified Eagle 's培养基，85 vol%水，1 % (w/v) PDEGMA-b-PMPC-b-PDEGMA, 2 % (w/v) d -脯氨酸，0.35 % (w/v) NaCl。冷冻保存3个月后，细胞恢复值保持稳定。最重要的是，在新开发的细胞冷冻培养基中冷冻保存后，iPSCs保持了其多能性。

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引用次数: 0

High-throughput microdroplet screening reveals chaperone- and time-dependent enhancement of recombinant papain folding and activity in Escherichia coli 高通量微滴筛选揭示了大肠杆菌中重组木瓜蛋白酶折叠和活性的伴侣性和时间依赖性增强

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-06 DOI: 10.1016/j.bej.2025.110042

Shimiao Chen , Yan Chen , Bin Shan , Yanyan Li , Fuhai Zheng , Yican Luo , Qinyu Lu

Recombinant papain production in Escherichia coli is limited by misfolding, aggregation, and host toxicity. Using a high-throughput microdroplet microbial culture (MMC) platform, we monitored chaperone-assisted folding and identified a transient “lysis window” during mid-log phase when activity peaked at 818.2 U/mg, doubling conventional yields. Co-expression with the Trigger Factor (TF) chaperone was most effective, whereas combining co-translational TF with the post-translational GroEL/ES system caused kinetic incompatibility, leading to massive aggregation and reduced activity (365.6 U/mg). Structural analyses revealed that optimal activity correlates with conformational flexibility rather than a static structure, a feature imparted by TF. Optimizing temporal dynamics and chaperone coordination is therefore essential for producing complex proteins. MMC provides a powerful platform for dissecting these folding pathways; in the redox-engineered E. coli SHuffle strain, this correct folding was synergistically enhanced, dramatically increasing soluble yields (to 450.2 mg/L) while maintaining high specific activity (857 U/mg) and confirming that an oxidizing cytoplasm is key for efficient production.

重组木瓜蛋白酶在大肠杆菌中的生产受到错误折叠、聚集和宿主毒性的限制。利用高通量微滴微生物培养（MMC）平台，我们监测了伴侣辅助折叠，并在中期发现了一个短暂的“裂解窗口”，当活性达到818.2 U/mg时，产量是常规产量的两倍。与触发因子（Trigger Factor， TF）伴侣共表达最有效，而与翻译后GroEL/ES系统共表达会导致动力学不相容，导致大量聚集和活性降低（365.6 U/mg）。结构分析表明，最佳活性与构象灵活性相关，而不是静态结构，这是TF赋予的特征。因此，优化时间动态和伴侣协调对于生产复杂蛋白质至关重要。MMC为解剖这些折叠通路提供了一个强大的平台；在氧化还原工程的大肠杆菌SHuffle菌株中，这种正确的折叠被协同增强，显著提高了可溶性产量（达到450.2 mg/L），同时保持了高比活性（857 U/mg），并证实了氧化细胞质是高效生产的关键。

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引用次数: 0

Characterization of hydrogels made of a novel recombinant spider silk proteins MaNC and evaluation for drug carriers 一种新型重组蛛丝蛋白MaNC水凝胶的表征及药物载体的评价

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-06 DOI: 10.1016/j.bej.2025.110040

Kangkang Wang , Guogeng Wang , Zhenzhou Zhao , Xujie Zhou , Hengrui Zhang , Na Li , Hongdan Bao

Owing to their good biocompatibility and low immunogenicity, spider silk proteins (spidroins) are promising biopolymers with high potential for various medical applications. Of these material types, spidroin-based hydrogels have attracted significant attention in biomaterials research owing to their non-toxic and excellent mechanical properties. However, reported spidroin hydrogels require extended gelation times. In this study, we engineered a novel recombinant spidroin (MaNC) by fusing the highly soluble N-terminal domain and the self-assembling C-terminal domain derived from major ampullate spidroin. The N-terminal domain confers high solubility on MaNC, while the C-terminal domain provides self-assembly properties, enabling rapid gelation of MaNC. We demonstrated that it spontaneously self-assembles into hydrogels at 37 °C within 10 min, accompanied by the formation of β-sheet structure, without requiring any external additives. It was shown that the self-assembling MaNC hydrogels exhibit no swelling, can be readily loaded with doxorubicin under aqueous conditions, release drug over one month, and that the diffusion rate can be tuned by changing the protein concentration and pH value. Collectively, these data support the notion that our silk hydrogels are fast-gelling, highly adaptable, and cytocompatible materials that self-assemble under mild conditions and are well suited for chemotherapy delivery.

由于其良好的生物相容性和低免疫原性，蜘蛛丝蛋白是一种很有前途的生物聚合物，在各种医学应用中具有很高的潜力。在这些材料类型中，蜘蛛蛋白水凝胶由于其无毒和优异的力学性能在生物材料研究中引起了极大的关注。然而，报道的蜘蛛蛋白水凝胶需要延长凝胶时间。在这项研究中，我们通过融合来自壶形蜘蛛蛋白的高可溶性n端结构域和自组装的c端结构域，构建了一种新的重组蜘蛛蛋白（MaNC）。n端结构域在MaNC上具有高溶解度，而c端结构域具有自组装特性，使MaNC能够快速凝胶化。我们证明了它在37°C下自发自组装成水凝胶，在10 min内，伴随着β-片结构的形成，而不需要任何外部添加剂。结果表明，自组装的MaNC水凝胶无溶胀现象，在水溶液条件下可以很容易地负载阿霉素，在一个月内释放药物，并且可以通过改变蛋白质浓度和pH值来调节扩散速率。总的来说，这些数据支持我们的丝绸水凝胶是一种快速凝胶化、适应性强、细胞相容性强的材料，在温和的条件下自组装，非常适合化疗递送。

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引用次数: 0

An adsorption-crosslinking immobilization approach for enhancing the operational stability of D-allulose 3-epimerase immobilized on chitosan-modified resin 壳聚糖改性树脂固定化D-allulose 3- epimase的吸附-交联固定化方法

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-03 DOI: 10.1016/j.bej.2025.110039

Yingying Zheng , Hongwei Liu , Shumin Guo , Jingwen Fei , Junqing Wang , Ruiming Wang , Piwu Li

D-allulose is a naturally occurring rare ketohexose that serves as a potential sucrose substitute. In this study, we developed a novel adsorption-crosslinking immobilization strategy based on a chitosan (CTS)-modified resin. After screening 15 resin types, D213 and ZGA351 were selected as carriers, and the CTS-modified carriers were crosslinked with glutaraldehyde to immobilize D-allulose 3-epimerase (DAE; EC 5.1.3.30). CTS modification significantly enhanced the performance of the immobilized enzymes, with the activity recovery rates of D213@CTS@DAE and ZGA351@CTS@DAE increasing compared to the unmodified carriers. The thermal, pH, and storage stabilities of the immobilized enzymes were significantly higher than those of free enzymes. D213@CTS@DAE retained high initial activity across multiple recycling and reuse cycles and sustained productivity in a packed-bed reactor after 29 days of continuous operation. The carrier material could be efficiently regenerated and reused. After regeneration, the immobilized enzyme maintained a high activity retention rate after five cycles. The findings provide a green biocatalytic solution for D-allulose production, which enhances the practicality and commercial viability of the immobilized enzyme.

D-allulose是一种天然存在的稀有酮己糖，可作为潜在的蔗糖替代品。在本研究中，我们开发了一种基于壳聚糖（CTS）改性树脂的新型吸附-交联固定策略。筛选15种树脂后，选择D213和ZGA351作为载体，将cts修饰的载体与戊二醛交联固定D-allulose 3- epimase （DAE; EC 5.1.3.30）。CTS修饰显著提高了固定化酶的性能，与未修饰的载体相比，D213@CTS@DAE和ZGA351@CTS@DAE的活性回收率提高。固定化酶的热稳定性、pH稳定性和贮藏稳定性均显著高于游离酶。D213@CTS@DAE在连续运行29天后，在填料床反应器中保持了高的初始活性，并在多个回收和再利用周期中保持了持续的生产力。载体材料可以有效地再生和再利用。再生后，固定化酶在5个循环后保持较高的活性保留率。该研究结果为D-allulose的生产提供了一种绿色的生物催化解决方案，提高了固定化酶的实用性和商业可行性。

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引用次数: 0

Addressing temperature gradient challenge in scaling up solid-state fermentation: A strategy using thermophilic strains for biosurfactant production 解决固态发酵中温度梯度的挑战：利用嗜热菌株生产生物表面活性剂的策略

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-02 DOI: 10.1016/j.bej.2025.110037

Jose Bueno-Mancebo, Adriana Artola, Syeda Amna Farooq, Raquel Barrena, Teresa Gea

Solid-state fermentation (SSF) is a promising approach for sustainable bioproduction production, particularly when using organic waste as a substrate. However, temperature gradients inherent to large-scale SSF often reduce process efficiency. This study developed a strategy for designing of a lipopeptide biosurfactant production process, scaling SSF in packed-bed bioreactors by thermophilic strains using winterisation oil cake (WOC) and sugarcane molasses (MOL) as nutrient sources. Fermentations at lab-scale (0.5 L) and pilot-scale (50 L) demonstrated a robust and reproducible process despite temperature changes from microbial activity. Among the strains tested, Bacillus subtilis CBI-7S1 was the highest producer, with optimal substrates amounts of 24 g of WOC and 12 g of MOL, producing 24.9 mg of crude lipopeptides per gram of dry matter (DM) at 0.5 L scale. These conditions were reproduced at pilot scale, with concentrations from 12 to 24 mg g⁻¹ DM. Moisture content strongly influenced biosurfactant production, while oxygen consumption was a reliable monitoring parameter. Surface tension was evaluated under different pH, temperature, and salinity, and mass spectrometry identified surfactin, iturin, and fengycin congeners. These findings provide insights into overcoming temperature gradients during scale-up and show that thermophilic strains enable biosurfactant production under SSF at pilot scale. This approach enhances SSF technological maturity, supporting its broader use in sustainable production of targeted metabolites across diverse bioprocesses.

固态发酵（SSF）是一种很有前途的可持续生物生产方法，特别是当使用有机废物作为底物时。然而，大规模SSF固有的温度梯度往往会降低工艺效率。本研究开发了一种设计脂肽生物表面活性剂生产工艺的策略，通过嗜热菌株在填充床生物反应器中以冬季油饼（WOC）和甘蔗糖蜜（MOL）为营养来源进行SSF的规模化。实验室规模（0.5 L）和中试规模（50 L）的发酵表明，尽管微生物活性引起温度变化，但发酵过程稳健且可重复。在试验菌株中，枯草芽孢杆菌CBI-7S1的产量最高，其最佳底物WOC为24 g， MOL为12 g，在0.5 L的条件下，每克干物质（DM）的粗脂肽产量为24.9 mg。这些条件在中试规模上重现，浓度从12到24 mg g−1 DM。水分含量强烈影响生物表面活性剂的生产，而氧气消耗是可靠的监测参数。在不同的pH、温度和盐度下评估表面张力，质谱鉴定表面蛋白、iturin和fengycin同系物。这些发现为在放大过程中克服温度梯度提供了见解，并表明嗜热菌株能够在SSF中试规模下生产生物表面活性剂。这种方法提高了SSF技术的成熟度，支持其在不同生物过程中可持续生产目标代谢物的更广泛应用。

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引用次数: 0

Biodegradation characteristics, pathway elucidation, and toxicity assessment of oxytetracycline-polyethylene combined pollution by two Aspergillus fungi 两种曲霉真菌对土霉素-聚乙烯复合污染的生物降解特性、途径阐明及毒性评价

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-02 DOI: 10.1016/j.bej.2025.110027

Yuan Zhang, Shuicao Liu, Tingting Ding, Wanwan Hao, Shuwen Ren, Jingwen Min, Congyang Zou

The widespread use of antibiotics and agricultural microplastics has become a global environmental issue, yet studies on the degradation of their combined pollution remain limited. Here, two functional strains, Aspergillus sp. DL-1 and DL-2, selected from 10 isolates and identified via ITS sequencing and phylogenetic analysis, were investigated for their ability to degrade oxytetracycline (OTC)–polyethylene (PE) combined pollution under optimal conditions. The OTC-PE combined treatment with fungi exhibited superior degradation performance. The OTC degradation rate in the OPD2 treatment reached 96.33 ± 0.36 %, while the weight loss rates of PE in the OPD1 and OPD2 were 10.83 ± 0.53 % and 8.92 ± 0.51 %, respectively. Furthermore, the WCA of the PE films in the combined treatments remained consistently lower than in the single-PE treatments, accompanied by significant changes in surface morphology and structure. Under the treatment of the strains, OTC can be degraded through multiple pathways, including decarbonylation, decarboxylation, deamination, demethylation, and dehydration. Specifically, biodegradation by DL-1 and DL-2 markedly lowered the developmental toxicity, mutagenicity, and bioaccumulation potential of OTC and its intermediates, thereby alleviating the environmental toxicity burden. In parallel, PE cleaved the chain under the action of microbial enzymes to generate low molecular hydrocarbons, which are further oxidized to carboxylic acids and other intermediate products. These findings provide a novel approach for addressing compound pollution in agricultural environments.

抗生素和农业微塑料的广泛使用已成为一个全球性的环境问题，但对其复合污染的降解研究仍然有限。本文从10株分离物中筛选出两株功能菌株Aspergillus sp. DL-1和DL-2，通过ITS测序和系统发育分析对其进行了鉴定，研究了它们在最佳条件下降解土霉素(OTC) -聚乙烯（PE）复合污染的能力。OTC-PE与真菌联合处理表现出较好的降解性能。OPD2治疗的场外降解率达到96.33 ±0.36  %,而减肥的PE OPD1和OPD2 10.83±0.53   %和8.92±0.51  %,分别。此外，复合处理PE膜的WCA始终低于单一PE处理，并伴有表面形貌和结构的显著变化。在菌株的作用下，OTC可通过多种途径降解，包括脱碳、脱羧、脱胺、去甲基和脱水。具体而言，DL-1和DL-2的生物降解显著降低了OTC及其中间体的发育毒性、诱变性和生物蓄积潜力，从而减轻了环境毒性负担。同时，PE在微生物酶的作用下裂解链，生成低分子碳氢化合物，再被氧化为羧酸等中间产物。这些发现为解决农业环境中的复合污染提供了一种新的途径。

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引用次数: 0

An exporter of Leucine, LeuE functions as an exporter of 1-aminocyclopropane-1-carboxylic acid in Escherichia coli 作为亮氨酸的出口商，LeuE在大肠杆菌中作为1-氨基环丙烷-1-羧酸的出口商

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-02 DOI: 10.1016/j.bej.2025.110038

Xue-Chun Li , Hong-Jie Xie , Zheng-Xiong Zhou , Bin Sun , Jun-Jie Lin , Xiao-Dan Li , Ren Wang

1-aminocyclopropane-1-carboxylic acid (ACC), a crucial intermediate in the ethylene biosynthetic pathway, plays a decisive role in regulating plant developmental processes and stress responses. In heterologous biosynthesis systems, ACC biosynthesis is inhibited due to its antibacterial activity. Enhancing ACC export confers advantages to ACC-producing biotechnological platforms and microbial cell factories. While bacterial transporters for canonical amino acids in Corynebacterium glutamicum and Escherichia coli have been extensively studied, their roles in ACC transport remain unexplored. To identify the exporters mediating ACC transport in E. coli, relevant experiments were performed in this study. The results showed that the leucine-exporter LeuE acts as a high-efficiency ACC exporter in E. coli. Subsequently, structural modeling coupled with site-directed mutagenesis revealed that L160 is a critical residue in determining the ACC transport specificity of LeuE. The L160Y mutation enhanced E. coli tolerance to ACC, elevating the threshold from 50 mM to 100 mM. Structural modeling predicted that the L160Y mutation-induced compaction reduced the transporter channel diameter by 28 % (from 9.551 Å to 6.876 Å), a change that may enhance the binding affinity of ACC. Notably, a significant reduction in intracellular ACC levels was observed in both the LeuE-overexpressing and LeuE (L160Y)-overexpressing strains. These findings enable large-scale biosynthesis of ACC and its derivatives via engineered microbial systems, thereby facilitating cost-effective development of ACC-based pharmaceuticals and agrochemicals.

1-氨基环丙烷-1-羧酸（1- aminocyclo丙烷-1-carboxylic acid， ACC）是乙烯生物合成途径中的重要中间体，在调控植物发育过程和逆境响应中起着决定性作用。在异源生物合成系统中，由于ACC的抗菌活性，其生物合成受到抑制。加强ACC出口可为ACC生产生物技术平台和微生物细胞工厂带来优势。虽然谷氨酸棒状杆菌和大肠杆菌中典型氨基酸的细菌转运体已被广泛研究，但它们在ACC转运中的作用仍未被探索。为了确定介导ACC在大肠杆菌中运输的出口蛋白，本研究进行了相关实验。结果表明，亮氨酸输出蛋白LeuE在大肠杆菌中具有高效的ACC输出蛋白作用。随后，结构建模与定点突变结合发现L160是决定LeuE ACC转运特异性的关键残基。L160Y突变增强了大肠杆菌对ACC的耐受性，将阈值从50 mM提高到100 mM。结构建模预测，L160Y突变诱导的压实使转运体通道直径减少了28% %（从9.551 Å减少到6.876 Å），这一变化可能增强了ACC的结合亲和力。值得注意的是，在LeuE-过表达和LeuE (L160Y)-过表达菌株中，细胞内ACC水平均显著降低。这些发现使通过工程微生物系统大规模生物合成ACC及其衍生物成为可能，从而促进基于ACC的药物和农用化学品的经济高效开发。

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引用次数: 0

Sustainable bioelectricity production in wetland-microbial fuel cells: The role of carbon-based wire and Echinodorus cordifolius as a nutrient source 湿地微生物燃料电池的可持续生物电生产：碳基金属丝和棘毛虫作为营养源的作用

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-02 DOI: 10.1016/j.bej.2025.110036

Azizuddin Muhammad Nashafi , Rujira Dolphen , Sucheewin Krobthong , Yodying Yingchutrakul , Chairat Treesubsuntorn

Achieving stable bioelectricity production in wetland-microbial fuel cells (WMFCs) remains challenging due to material degradation and fluctuating environmental conditions. This study investigates the long-term performance of carbon-based electrodes and wires in WMFC systems by assessing cathodic physiochemical properties and rhizosphere metabolomics under light (700 μmol·m⁻²·s⁻¹) and dark conditions. Over 150 days, carbon-based wire systems generated 3.7 times higher bioelectricity than commercial copper-based wires. By the final day, the Plant + Carbon wire system achieved a power density of 31.71 ± 7.11 mW/m², compared to 8.59 ± 5.35 mW/m² in the Plant + Copper wire system. Light intensity and cathodic temperature strongly influenced bioelectricity, with higher generation during the light period (8.28 ± 2.93–12.29 ± 5.56 mW/m²) than in darkness (7.08 ± 3.27–7.15 ± 4.26 mW/m²). Interestingly, planted systems consistently exhibited more stable power generation than unplanted systems, likely due to enhanced rhizosphere activity and distinctive metabolite profiles that supported electron transfer and temperature adaptation. Metabolomic analysis revealed up-regulated metabolites, including 10-undecenoic acid and carnitine derivatives, which may function as nutrients, electron acceptors, and thermoprotectants under diurnal temperature fluctuations. These findings highlight the role of wetland plants and carbon-based materials in improving WMFC resilience, ensuring operational stability, and enabling long-term bioelectricity generation.

由于材料降解和波动的环境条件，在湿地微生物燃料电池（WMFCs）中实现稳定的生物发电仍然具有挑战性。本研究通过评估在光照（700 μmol·m⁻²·s⁻¹）和黑暗条件下的阴极物理化学性质和根际代谢组学，研究了碳基电极和导线在WMFC系统中的长期性能。在150天的时间里，碳基电线系统产生的生物电是商用铜基电线的3.7倍。到最后一天，Plant + 碳线系统的功率密度达到31.71 ± 7.11 mW/m²，而Plant + 铜线系统的功率密度为8.59 ± 5.35 mW/m²。光强度和阴极温度的强烈影响生物电,高代光期间(8.28 ±2.93 - -12.29  ±5.56  mW / m²)比在黑暗中(7.08 ±3.27 - -7.15  ±4.26  mW / m²)。有趣的是，种植系统始终表现出比未种植系统更稳定的发电，这可能是由于根际活性增强和支持电子转移和温度适应的独特代谢物谱。代谢组学分析显示，在昼夜温度波动下，10-十一烯酸和肉碱衍生物等代谢物可能具有营养物质、电子受体和热保护剂的功能。这些发现强调了湿地植物和碳基材料在提高WMFC弹性、确保运行稳定性和实现长期生物发电方面的作用。

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引用次数: 0

Effects of electrolyte composition and applied voltage on methane generation and microbial community shifts in the electromethanogenesis system 电解液组成和外加电压对甲烷电生成系统中甲烷生成和微生物群落迁移的影响

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-11-28 DOI: 10.1016/j.bej.2025.110035

Lucky Caesar Direstiyani, Daisuke Inoue, Michihiko Ike

An electromethanogenesis (EM) system was successfully established using non-acclimated anaerobic digestion sludge as the inoculum. This study aimed to evaluate the EM performance by varying the electrolyte composition and the applied voltage. Alterations in the microbial community associated with CH₄ generation and bioelectrochemchemical performance were also investigated. The findings indicated that the use of organic-rich electrolyte with a low applied voltage of 0.15 V showed a positive correlation with enhanced CH₄ generation up to 59 % and a CH₄ yield of 223.13 mmol day⁻¹ m⁻² which was ten times higher than the operation using the same electrolyte with an applied voltage of 0.35 V. Microbial community analysis revealed a shift of dominant methanogens from Methanosaeta to Methanosarcina and Methanoculleus at the cathodic biofilms when operated with organic-rich electrolyte at low voltage of 0.15 V. The presence of electroactive bacteria, such as DMER64 and JGI-0000079-D21, and syntrophic bacteria, including Desulfovibrio and Petrimonas, suggested the development of syntrophic interactions that strengthen biofilm resilience and the overall performance of the EM system. The microbial interaction network also emphasized the significance of electrolyte composition and adequate applied voltage in shaping microbial biofilms for efficient CH₄ generation. The findings of this study accentuate the roles of sufficient electrolyte composition and low-voltage in enhancing the EM performance and corroborate the synergistic advantages of the EM system.

以未驯化的厌氧消化污泥为接种物，成功建立了电产甲烷（EM）系统。本研究旨在通过改变电解液成分和施加电压来评估电磁性能。微生物群落的变化与CH4的产生和生物电化学性能也进行了研究。结果表明，使用低电压为0.15 V的富有机电解液，CH4生成率可提高59 %，CH4产率为223.13 mmol day−1 m−2，比使用相同电压为0.35 V的电解液提高10倍。微生物群落分析表明，在0.15 V富有机物电解液作用下，阴极生物膜上优势产甲烷菌由Methanosaeta向Methanosarcina和Methanoculleus转变。电活性细菌（如DMER64和JGI-0000079-D21）和共生细菌（包括Desulfovibrio和Petrimonas）的存在表明，共生相互作用的发展增强了生物膜的弹性和EM系统的整体性能。微生物相互作用网络还强调了电解质组成和适当的施加电压在形成微生物生物膜以有效生成CH4方面的重要性。本研究的结果强调了充足的电解质成分和低电压对提高电磁性能的作用，并证实了电磁系统的协同优势。

{"title":"Effects of electrolyte composition and applied voltage on methane generation and microbial community shifts in the electromethanogenesis system","authors":"Lucky Caesar Direstiyani, Daisuke Inoue, Michihiko Ike","doi":"10.1016/j.bej.2025.110035","DOIUrl":"10.1016/j.bej.2025.110035","url":null,"abstract":"<div><div>An electromethanogenesis (EM) system was successfully established using non-acclimated anaerobic digestion sludge as the inoculum. This study aimed to evaluate the EM performance by varying the electrolyte composition and the applied voltage. Alterations in the microbial community associated with CH<sub>4</sub> generation and bioelectrochemchemical performance were also investigated. The findings indicated that the use of organic-rich electrolyte with a low applied voltage of 0.15 V showed a positive correlation with enhanced CH<sub>4</sub> generation up to 59 % and a CH<sub>4</sub> yield of 223.13 mmol day<sup>−1</sup> m<sup>−2</sup> which was ten times higher than the operation using the same electrolyte with an applied voltage of 0.35 V. Microbial community analysis revealed a shift of dominant methanogens from <em>Methanosaeta</em> to <em>Methanosarcina</em> and <em>Methanoculleus</em> at the cathodic biofilms when operated with organic-rich electrolyte at low voltage of 0.15 V. The presence of electroactive bacteria, such as <em>DMER64</em> and <em>JGI-0000079-D21</em>, and syntrophic bacteria, including <em>Desulfovibrio</em> and <em>Petrimonas</em>, suggested the development of syntrophic interactions that strengthen biofilm resilience and the overall performance of the EM system. The microbial interaction network also emphasized the significance of electrolyte composition and adequate applied voltage in shaping microbial biofilms for efficient CH<sub>4</sub> generation. The findings of this study accentuate the roles of sufficient electrolyte composition and low-voltage in enhancing the EM performance and corroborate the synergistic advantages of the EM system.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"227 ","pages":"Article 110035"},"PeriodicalIF":3.7,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145682446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0