Biochemical Engineering Journal最新文献_第4页

Efficient production of 9α-hydroxy-steroid from phytosterols in Mycobacterium fortuitum ATCC 6842 by modifying multiple genes

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-18 DOI: 10.1016/j.bej.2025.109689

Ruijie Zhang , Wen Sun , Suwan Han , Xiaoxuan Sun , Xinghui Zhai , Beiru He , Xianfeng Zhu , Baoguo Zhang

The steroid drug industry is increasingly utilizing microbial biotransformation, employing genetically modified mycobacteria to convert phytosterols into steroid intermediates, with an emphasis on improving yield and purity. This study enhances the production of 9α-hydroxy-4-androstene-3,17-dione (9-OH-AD), a vital C19 steroid intermediate for glucocorticoid synthesis, by genetically modifying Mycobacterium fortuitum ATCC 6842. The study involved the targeted disruption of five 3-ketosteroid-Δ¹-dehydrogenase (kstD) genes to prevent Δ¹-dehydrogenation. The purity of 9-OH-AD is initially low at 81.85 % due to two main by-products: 9,22-dihydroxy-23,24-bisnorchol-4-ene-3-one (9-OH-HP) and 9,24-dihydroxychol-4-en-3-one (9,24-DHC). To address this, the steroid aldolase (sal) gene was deleted to block the C22 metabolic pathway, which completely eliminated 9-OH-HP and increased the purity of 9-OH-AD to 88.19 %. To further reduce 9,24-DHC levels, acyl-CoA dehydrogenases ChsE1 and ChsE2 were overexpressed. The resulting strain, MFKS_chsE1-chsE2, achieved a high purity of 9-OH-AD at 94.96 %, with a molar yield of 87.17 % from 10 g/L phytosterols, and converted up to 30 g/L of phytosterols into 15.91 g/L of 9-OH-AD. This method effectively enhances both the production and purity of this important steroid intermediate.

{"title":"Efficient production of 9α-hydroxy-steroid from phytosterols in Mycobacterium fortuitum ATCC 6842 by modifying multiple genes","authors":"Ruijie Zhang , Wen Sun , Suwan Han , Xiaoxuan Sun , Xinghui Zhai , Beiru He , Xianfeng Zhu , Baoguo Zhang","doi":"10.1016/j.bej.2025.109689","DOIUrl":"10.1016/j.bej.2025.109689","url":null,"abstract":"<div><div>The steroid drug industry is increasingly utilizing microbial biotransformation, employing genetically modified mycobacteria to convert phytosterols into steroid intermediates, with an emphasis on improving yield and purity. This study enhances the production of 9α-hydroxy-4-androstene-3,17-dione (9-OH-AD), a vital C19 steroid intermediate for glucocorticoid synthesis, by genetically modifying <em>Mycobacterium fortuitum</em> ATCC 6842. The study involved the targeted disruption of five 3-ketosteroid-Δ<sup>1</sup>-dehydrogenase (<em>kstD</em>) genes to prevent Δ<sup>1</sup>-dehydrogenation. The purity of 9-OH-AD is initially low at 81.85 % due to two main by-products: 9,22-dihydroxy-23,24-bisnorchol-4-ene-3-one (9-OH-HP) and 9,24-dihydroxychol-4-en-3-one (9,24-DHC). To address this, the steroid aldolase (<em>sal</em>) gene was deleted to block the C22 metabolic pathway, which completely eliminated 9-OH-HP and increased the purity of 9-OH-AD to 88.19 %. To further reduce 9,24-DHC levels, acyl-CoA dehydrogenases ChsE1 and ChsE2 were overexpressed. The resulting strain, MFKS_<em>chsE1</em>-<em>chsE2</em>, achieved a high purity of 9-OH-AD at 94.96 %, with a molar yield of 87.17 % from 10 g/L phytosterols, and converted up to 30 g/L of phytosterols into 15.91 g/L of 9-OH-AD. This method effectively enhances both the production and purity of this important steroid intermediate.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"218 ","pages":"Article 109689"},"PeriodicalIF":3.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous expression of algal fucosyltransferases and sulfotransferases in Escherichia coli

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-18 DOI: 10.1016/j.bej.2025.109690

Ahmed Zayed , Benjamin Ledermann , Thomas Fischöder , Lothar Elling , Nicole Frankenberg-Dinkel , Roland Ulber

Alternative to classical production of fucoidans, enzymatic synthesis has been proposed especially following genomic and bioinformatic investigations in Ectocarpus siliculosus and Saccharina sp. The present research aimed to assess the activity of four putative algal enzymes, including fucosyltransferases (FucTs_21 and FucTs_50) and carbohydrate sulfotransferases (STs_32 and STs_283) expressed in E. coli BL21 (DE3) for future application in lab-synthesized fucoidans. A specific activity of 0.47 pmol.min⁻¹.µg⁻¹ was observed for FucTs_50 in the Glycosyltransferase activity assay, while multiplexed capillary electrophoresis (MP-CE) revealed that almost 32 % of GDP-L-fucose was affected. Additionally, the Universal Sulfotransferase Activity Kit was used to evaluate STs, and the results indicated that STs_283 and STs_32 exhibited specific activities at 2.29 and 2.38 pmol.min⁻¹.µg⁻¹, respectively. The results indicated correctly folded active enzymes with binding sites specific to their corresponding substrates.

{"title":"Heterologous expression of algal fucosyltransferases and sulfotransferases in Escherichia coli","authors":"Ahmed Zayed , Benjamin Ledermann , Thomas Fischöder , Lothar Elling , Nicole Frankenberg-Dinkel , Roland Ulber","doi":"10.1016/j.bej.2025.109690","DOIUrl":"10.1016/j.bej.2025.109690","url":null,"abstract":"<div><div>Alternative to classical production of fucoidans, enzymatic synthesis has been proposed especially following genomic and bioinformatic investigations in <em>Ectocarpus siliculosus</em> and <em>Saccharina</em> sp. The present research aimed to assess the activity of four putative algal enzymes, including fucosyltransferases (FucTs_21 and FucTs_50) and carbohydrate sulfotransferases (STs_32 and STs_283) expressed in <em>E. coli</em> BL21 (DE3) for future application in lab-synthesized fucoidans. A specific activity of 0.47 pmol.min<sup>−1</sup>.µg<sup>−1</sup> was observed for FucTs_50 in the Glycosyltransferase activity assay, while multiplexed capillary electrophoresis (MP-CE) revealed that almost 32 % of GDP-L-fucose was affected. Additionally, the Universal Sulfotransferase Activity Kit was used to evaluate STs, and the results indicated that STs_283 and STs_32 exhibited specific activities at 2.29 and 2.38 pmol.min<sup>−1</sup>.µg<sup>−1</sup>, respectively. The results indicated correctly folded active enzymes with binding sites specific to their corresponding substrates.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"218 ","pages":"Article 109690"},"PeriodicalIF":3.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced nitrogen removal through rapid biofilm formation by Paracoccus sp. XN-1 immobilized carriers in sequencing batch biofilm reactor

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-18 DOI: 10.1016/j.bej.2025.109678

Yuhang Song , Lingmin Zhao , Jiaonan Zhang , Jiaoling Zhang , Qingpi Yan

Biofilm formation is critical for wastewater treatment efficiency, but slow biofilm development often limits system startup and performance. In this study, a sequencing batch biofilm reactor (SBBR) was established to evaluate Paracoccus sp. XN-1's capability to accelerate biofilm formation for treating aquaculture wastewater. Results showed that XN-1 inoculation (T-SBR) significantly enhanced biofilm formation, achieving mature biofilm development within 36 days compared to 48 days in conventional activated sludge (SBR). This rapid startup is particularly valuable for aquaculture facilities that require efficient and stable wastewater treatment systems to maintain water quality during intensive farming. High-throughput sequencing revealed distinct microbial community structures between the two systems, with T-SBR showing enrichment of key functional groups, particularly Methyloparacoccus (4.8 %) and Lysinibacillus (21.4 %). Network analysis demonstrated complex ecological interactions within T-SBR, with Methyloparacoccus forming a central hub coordinating nitrogen transformation processes. Functional prediction indicated enhanced nitrogen metabolism and oxidative phosphorylation pathways in T-SBR, contributing to superior NH₄⁺-N (99.27 %) and TN (83.14 %) removal efficiencies. These results demonstrate that XN-1 inoculation optimizes microbial community assembly for enhanced nitrogen removal, providing a practical solution for sustainable aquaculture wastewater treatment.

{"title":"Enhanced nitrogen removal through rapid biofilm formation by Paracoccus sp. XN-1 immobilized carriers in sequencing batch biofilm reactor","authors":"Yuhang Song , Lingmin Zhao , Jiaonan Zhang , Jiaoling Zhang , Qingpi Yan","doi":"10.1016/j.bej.2025.109678","DOIUrl":"10.1016/j.bej.2025.109678","url":null,"abstract":"<div><div>Biofilm formation is critical for wastewater treatment efficiency, but slow biofilm development often limits system startup and performance. In this study, a sequencing batch biofilm reactor (SBBR) was established to evaluate <em>Paracoccus</em> sp. XN-1's capability to accelerate biofilm formation for treating aquaculture wastewater. Results showed that XN-1 inoculation (T-SBR) significantly enhanced biofilm formation, achieving mature biofilm development within 36 days compared to 48 days in conventional activated sludge (SBR). This rapid startup is particularly valuable for aquaculture facilities that require efficient and stable wastewater treatment systems to maintain water quality during intensive farming. High-throughput sequencing revealed distinct microbial community structures between the two systems, with T-SBR showing enrichment of key functional groups, particularly <em>Methyloparacoccus</em> (4.8 %) and <em>Lysinibacillus</em> (21.4 %). Network analysis demonstrated complex ecological interactions within T-SBR, with <em>Methyloparacoccus</em> forming a central hub coordinating nitrogen transformation processes. Functional prediction indicated enhanced nitrogen metabolism and oxidative phosphorylation pathways in T-SBR, contributing to superior NH<sub>4</sub><sup>+</sup>-N (99.27 %) and TN (83.14 %) removal efficiencies. These results demonstrate that XN-1 inoculation optimizes microbial community assembly for enhanced nitrogen removal, providing a practical solution for sustainable aquaculture wastewater treatment.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109678"},"PeriodicalIF":3.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning-based predictive modeling and optimization: Artificial neural network-genetic algorithm vs. response surface methodology for black soldier fly (Hermetia illucens) farm waste fermentation

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-17 DOI: 10.1016/j.bej.2025.109685

Oseweuba Valentine Okoro , D.E. Caevel Hippolyte , Lei Nie , Keikhosro Karimi , Joeri F.M. Denayer , Armin Shavandi

Recognizing the complexity of non-linear and interdependent biological processes, this study compared the predictive performance of artificial neural network (ANN) models with response surface methodology regression based (RB) models. The research focused on the biological transformation of black soldier fly (Hermetia illucens) farm waste into chitin, facilitated by Lactobacillus paracasei. Key parameters of time (1–7 days), temperature (30–40 °C), substrate concentration (7.5–20 wt%), and inoculum concentration (5–15 v/v%), were evaluated for their impact on demineralization and deproteinization subprocesses and subsequently optimized. It was determined that the ANN models outperformed RB models, with R² values of 0.950 and 0.959 for DP% and DD%, compared to 0.677 and 0.720 for RB models. While both models, optimized using a multi-objective genetic algorithm (MOGA) and a desirability function respectively, produced comparable optimal results, differences emerged in process variable analysis. Main effects plots (RB) and one way partial dependence plots (ANN) revealed conflicting parameter influences, highlighting the limitations of regression models in complex systems. This study highlights the superiority of ANN-MOGA in addressing biological complexity and recommends its use especially if RB models show suboptimal predictive capabilities.

{"title":"Machine learning-based predictive modeling and optimization: Artificial neural network-genetic algorithm vs. response surface methodology for black soldier fly (Hermetia illucens) farm waste fermentation","authors":"Oseweuba Valentine Okoro , D.E. Caevel Hippolyte , Lei Nie , Keikhosro Karimi , Joeri F.M. Denayer , Armin Shavandi","doi":"10.1016/j.bej.2025.109685","DOIUrl":"10.1016/j.bej.2025.109685","url":null,"abstract":"<div><div>Recognizing the complexity of non-linear and interdependent biological processes, this study compared the predictive performance of artificial neural network (ANN) models with response surface methodology regression based (RB) models. The research focused on the biological transformation of black soldier fly (<em>Hermetia illucens</em>) farm waste into chitin, facilitated by <em>Lactobacillus paracasei</em>. Key parameters of time (1–7 days), temperature (30–40 °C), substrate concentration (7.5–20 wt%), and inoculum concentration (5–15 v/v%), were evaluated for their impact on demineralization and deproteinization subprocesses and subsequently optimized. It was determined that the ANN models outperformed RB models, with R² values of 0.950 and 0.959 for DP% and DD%, compared to 0.677 and 0.720 for RB models. While both models, optimized using a multi-objective genetic algorithm (MOGA) and a desirability function respectively, produced comparable optimal results, differences emerged in process variable analysis. Main effects plots (RB) and one way partial dependence plots (ANN) revealed conflicting parameter influences, highlighting the limitations of regression models in complex systems. This study highlights the superiority of ANN-MOGA in addressing biological complexity and recommends its use especially if RB models show suboptimal predictive capabilities.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"218 ","pages":"Article 109685"},"PeriodicalIF":3.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the antibiotics removal ability of Monoraphidium contortum

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-17 DOI: 10.1016/j.bej.2025.109686

Larissa T.A. Costa-Ramos , Marcus V.X. Senra , Guilherme H.S. Nogueira , Ralf R. Ramalho-Júnior , Sandro J. Andrade , Evellin E. Santo , Aline K. Gohara-Beirigo , Juliana Gomes Santos , Livia S. Ferreira-Camargo , João Carlos M. Carvalho , Marcelo C. Matsudo

This study shows the Monoraphidium contortum potential for removing antibiotics, specifically sulfamethoxazole (SMX) and trimethoprim (TMP), from wastewater. Experiments were conducted using a bench-scale tubular photobioreactor, where the microalga demonstrated the ability to reduce antibiotic concentrations while simultaneously removing inorganic nutrients and producing commercially valuable biomass. When each antibiotic was individually added in the culture medium, M. contortum was able to remove 42.3 % of SMX and 28.6 % of TMP. However, when both antibiotics were introduced simultaneously, SMX removal decreased to 7 %. Additionally, the biochemical composition of the resulting biomass revealed stable lipid content, with a fatty acid profile suitable for biodiesel production. By genome analysis, it was possible to identify a multicopper oxidase enzyme, with potential antibiotic-degrading properties, shedding light on the mechanisms behind the removal process. These findings suggest that M. contortum could play a crucial role in bioremediation and sustainable wastewater treatment, generating feedstock for bioenergy production.

{"title":"Unveiling the antibiotics removal ability of Monoraphidium contortum","authors":"Larissa T.A. Costa-Ramos , Marcus V.X. Senra , Guilherme H.S. Nogueira , Ralf R. Ramalho-Júnior , Sandro J. Andrade , Evellin E. Santo , Aline K. Gohara-Beirigo , Juliana Gomes Santos , Livia S. Ferreira-Camargo , João Carlos M. Carvalho , Marcelo C. Matsudo","doi":"10.1016/j.bej.2025.109686","DOIUrl":"10.1016/j.bej.2025.109686","url":null,"abstract":"<div><div>This study shows the <em>Monoraphidium contortum</em> potential for removing antibiotics, specifically sulfamethoxazole (SMX) and trimethoprim (TMP), from wastewater. Experiments were conducted using a bench-scale tubular photobioreactor, where the microalga demonstrated the ability to reduce antibiotic concentrations while simultaneously removing inorganic nutrients and producing commercially valuable biomass. When each antibiotic was individually added in the culture medium, <em>M. contortum</em> was able to remove 42.3 % of SMX and 28.6 % of TMP. However, when both antibiotics were introduced simultaneously, SMX removal decreased to 7 %. Additionally, the biochemical composition of the resulting biomass revealed stable lipid content, with a fatty acid profile suitable for biodiesel production. By genome analysis, it was possible to identify a multicopper oxidase enzyme, with potential antibiotic-degrading properties, shedding light on the mechanisms behind the removal process. These findings suggest that <em>M. contortum</em> could play a crucial role in bioremediation and sustainable wastewater treatment, generating feedstock for bioenergy production.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109686"},"PeriodicalIF":3.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable intensified conversion of waste corn cob to 5-HMF by synergistic action between infrared radiation and cellulase grafted nano-titania-silica bio-photocatalyst

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-16 DOI: 10.1016/j.bej.2025.109675

Sohini Roy Choudhury, Rajat Chakraborty

This study reports the pioneering investigation into the synergistic effects of nano-silica-titania immobilized cellulase bio-photocatalyst and visible near-infrared radiation (VIS-NIR) on the intensification of 5-hydroxymethylfurfural (5-HMF) synthesis from delignified corn cob (DCC). A nano-bio-photocatalyst, silica/titania-cellulase (ST-C),was engineered where nano-titania-silica core-shell spheres were synthesized and conjugated with Aspergillus niger derived cellulase enzyme. With a band gap of 2.83 eV, the optimized ST-C exhibited its potential for enhanced bio-photocatalytic efficacy in 5-HMF synthesis via bio-photochemical conversion (BPC) of DCC in a quartz iodine irradiated self-vibrating batch bioreactor (QIISBR) (100 W,visible range:380–700 nm).The synergistic effect between VIS-NIR of QIISBR and the prepared ST-C bio-photocatalyst was evident from the enhanced 5-HMF yield(74.24 mol%) compared to dark run (69.3 mol%) at the optimal 60°C BPC temperature, 1 wt% bio-photocatalyst concentration, 60 min reaction time, 30 w/w Biomass-to-Water ratio as determined by Taguchi Structured Orthogonal Design (TSOD). The VIS-NIR system also offered 68.5 % energy savings compared to dark run. Remarkably,the immobilized enzyme preserved 93 % of its initial activity after nine consecutive uses, showcasing its high operational stability and recyclability attributes. The Life Cycle Sustainability Evaluation (LCSE) revealed reduction in environmental burdens, marked by 69.23 % decrease in Carbon Footprint,45.8 % decline in Human Toxicity Potential,50.1 % reduction in Water Consumption Potential compared to conventional process.The developed process demonstrating synergistic application of VIS-NIR and bio-photocatalyst could procreate a greener pathway through sustainable valorization of lignocellulosic biomass towards synthesis of 5-HMF and similar platform chemicals.

{"title":"Sustainable intensified conversion of waste corn cob to 5-HMF by synergistic action between infrared radiation and cellulase grafted nano-titania-silica bio-photocatalyst","authors":"Sohini Roy Choudhury, Rajat Chakraborty","doi":"10.1016/j.bej.2025.109675","DOIUrl":"10.1016/j.bej.2025.109675","url":null,"abstract":"<div><div>This study reports the pioneering investigation into the synergistic effects of nano-silica-titania immobilized cellulase bio-photocatalyst and visible near-infrared radiation (VIS-NIR) on the intensification of 5-hydroxymethylfurfural (5-HMF) synthesis from delignified corn cob (DCC). A nano-bio-photocatalyst, silica/titania-cellulase (ST-C),was engineered where nano-titania-silica core-shell spheres were synthesized and conjugated with <em>Aspergillus niger</em> derived cellulase enzyme. With a band gap of 2.83 eV, the optimized ST-C exhibited its potential for enhanced bio-photocatalytic efficacy in 5-HMF synthesis via bio-photochemical conversion (BPC) of DCC in a quartz iodine irradiated self-vibrating batch bioreactor (QIISBR) (100 W,visible range:380–700 nm).The synergistic effect between VIS-NIR of QIISBR and the prepared ST-C bio-photocatalyst was evident from the enhanced 5-HMF yield(74.24 mol%) compared to dark run (69.3 mol%) at the optimal 60°C BPC temperature, 1 wt% bio-photocatalyst concentration, 60 min reaction time, 30 w/w Biomass-to-Water ratio as determined by Taguchi Structured Orthogonal Design (TSOD). The VIS-NIR system also offered 68.5 % energy savings compared to dark run. Remarkably,the immobilized enzyme preserved 93 % of its initial activity after nine consecutive uses, showcasing its high operational stability and recyclability attributes. The Life Cycle Sustainability Evaluation (LCSE) revealed reduction in environmental burdens, marked by 69.23 % decrease in Carbon Footprint,45.8 % decline in Human Toxicity Potential,50.1 % reduction in Water Consumption Potential compared to conventional process.The developed process demonstrating synergistic application of VIS-NIR and bio-photocatalyst could procreate a greener pathway through sustainable valorization of lignocellulosic biomass towards synthesis of 5-HMF and similar platform chemicals.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109675"},"PeriodicalIF":3.7,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Degradation of β-HCH by Enterobacter sp. CS01: Characteristics, mechanism and application in soil remediation 肠杆菌 CS01 对 β-HCH 的降解：特性、机理及在土壤修复中的应用

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-15 DOI: 10.1016/j.bej.2025.109673

Huijun Shi , Qing Chen , Yanpeng Liang , Litang Qin , Honghu Zeng , Xiaohong Song

β-Hexachlorocyclohexane (β-HCH) is a persistent organochlorine pesticide that poses a significant threat to the ecological environment, necessitating the urgent development of effective degradation methods. Microbial degradation has demonstrated substantial potential among various bioremediation techniques due to its environmentally friendly and economical characteristics. This study evaluates the degradation capability of Enterobacter sp. CS01 on β-HCH, its physiological responses, and its potential application in soil remediation. Under optimal conditions (pH 7, 30°C), 51 % of β-HCH was effectively removed. Metabolomics and antioxidant enzyme activity analyses revealed that CS01 defends against oxidative damage by modulating the activities of superoxide dismutase (SOD) and catalase (CAT), involving butyrate, alanine, aspartate, and glutamate metabolism, as well as the pentose phosphate pathway. CS01 converts β-HCH into less toxic intermediates through dichloride elimination, dehalogenation of hydrogen, and hydrolysis reactions. Soil experiments indicated that soil enzyme activities (S-POD, S-DHA, S-PPO) are closely related to the degradation of β-HCH, with the order of carbon source utilization being esters, amino acids, and sugars. This study provides new insights into the microbial degradation mechanisms of organochlorine pesticides and aids in the development of more efficient and environmentally friendly degradation technologies.

{"title":"Degradation of β-HCH by Enterobacter sp. CS01: Characteristics, mechanism and application in soil remediation","authors":"Huijun Shi , Qing Chen , Yanpeng Liang , Litang Qin , Honghu Zeng , Xiaohong Song","doi":"10.1016/j.bej.2025.109673","DOIUrl":"10.1016/j.bej.2025.109673","url":null,"abstract":"<div><div>β-Hexachlorocyclohexane (β-HCH) is a persistent organochlorine pesticide that poses a significant threat to the ecological environment, necessitating the urgent development of effective degradation methods. Microbial degradation has demonstrated substantial potential among various bioremediation techniques due to its environmentally friendly and economical characteristics. This study evaluates the degradation capability of <em>Enterobacter</em> sp. CS01 on β-HCH, its physiological responses, and its potential application in soil remediation. Under optimal conditions (pH 7, 30°C), 51 % of β-HCH was effectively removed. Metabolomics and antioxidant enzyme activity analyses revealed that CS01 defends against oxidative damage by modulating the activities of superoxide dismutase (SOD) and catalase (CAT), involving butyrate, alanine, aspartate, and glutamate metabolism, as well as the pentose phosphate pathway. CS01 converts β-HCH into less toxic intermediates through dichloride elimination, dehalogenation of hydrogen, and hydrolysis reactions. Soil experiments indicated that soil enzyme activities (S-POD, S-DHA, S-PPO) are closely related to the degradation of β-HCH, with the order of carbon source utilization being esters, amino acids, and sugars. This study provides new insights into the microbial degradation mechanisms of organochlorine pesticides and aids in the development of more efficient and environmentally friendly degradation technologies.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109673"},"PeriodicalIF":3.7,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of electric field on microbial metabolism in petroleum-polluted soil: Insights from microbial function and carbon utilization characteristics 电场对石油污染土壤中微生物代谢的影响：从微生物功能和碳利用特征看问题

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-13 DOI: 10.1016/j.bej.2025.109665

Ruijuan Fan , Mengke Zhu , Bin Yang , Xingfu Yan

This study investigated how the electric field enhances microbial metabolism during bio-electrokinetic (BIO-EK) remediation and identified factors hindering sustainable pollutant degradation. The results showed that the degradation rate of total organic carbon (TOC) was higher in BIO-EK than in bioremediation and electrokinetic processes, by up to 2.22 and 1.54 times, respectively. Similarly, the degradation efficiency of total petroleum hydrocarbons (TPH) in BIO-EK was 1.58 and 1.81 times higher than in the bioremediation and electrokinetic groups. In BIO-EK, microbial biomass carbon (MBC) content was up to 6.13 times higher than in the bioremediation group, and dissolved organic carbon (DOC) content was 2.30 and 1.26 times higher than in the bioremediation and electrokinetic groups, respectively. This indicates that the electric field promoted the conversion of TOC to DOC, which was assimilated by microorganisms to generate MBC. The analysis of the structure and functional genes of soil microbial communities revealed that the electric field accelerated the degradation of key petroleum hydrocarbon components and the soil carbon cycle. However, degradation rates varied, and MBC and DOC levels declined in later stages, indicating discontinuous pollutant degradation. The analysis of the ratio of organic carbon derived from petroleum and soil sources (¹²Cp/¹²Cs) revealed intermittent use of petroleum-derived carbon, potentially limiting the sustainable degradation of pollutants. The research results provide insights for improving BIO-EK remediation efficiency.

{"title":"Effects of electric field on microbial metabolism in petroleum-polluted soil: Insights from microbial function and carbon utilization characteristics","authors":"Ruijuan Fan , Mengke Zhu , Bin Yang , Xingfu Yan","doi":"10.1016/j.bej.2025.109665","DOIUrl":"10.1016/j.bej.2025.109665","url":null,"abstract":"<div><div>This study investigated how the electric field enhances microbial metabolism during bio-electrokinetic (BIO-EK) remediation and identified factors hindering sustainable pollutant degradation. The results showed that the degradation rate of total organic carbon (TOC) was higher in BIO-EK than in bioremediation and electrokinetic processes, by up to 2.22 and 1.54 times, respectively. Similarly, the degradation efficiency of total petroleum hydrocarbons (TPH) in BIO-EK was 1.58 and 1.81 times higher than in the bioremediation and electrokinetic groups. In BIO-EK, microbial biomass carbon (MBC) content was up to 6.13 times higher than in the bioremediation group, and dissolved organic carbon (DOC) content was 2.30 and 1.26 times higher than in the bioremediation and electrokinetic groups, respectively. This indicates that the electric field promoted the conversion of TOC to DOC, which was assimilated by microorganisms to generate MBC. The analysis of the structure and functional genes of soil microbial communities revealed that the electric field accelerated the degradation of key petroleum hydrocarbon components and the soil carbon cycle. However, degradation rates varied, and MBC and DOC levels declined in later stages, indicating discontinuous pollutant degradation. The analysis of the ratio of organic carbon derived from petroleum and soil sources (<sup>12</sup>Cp/<sup>12</sup>Cs) revealed intermittent use of petroleum-derived carbon, potentially limiting the sustainable degradation of pollutants. The research results provide insights for improving BIO-EK remediation efficiency.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109665"},"PeriodicalIF":3.7,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A model based approach for monitoring Bordetella pertussis fermentation with an inline spectro-fluorescence probe

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-13 DOI: 10.1016/j.bej.2025.109674

Abhishek Mishra , Michael Vitelli , Boris Tartakovsky , Ibrahim M. Tamer , Hector Budman

Presently, the extraction of the antigen pertactin poses a challenge in the manufacturing of the whooping cough vaccine due to its low and variable yield [1]. In this work, a hybrid model that combines empirical and mechanistic parts and in-line fluorescence measurements is used to design an estimator for monitoring the manufacturing process in bioreactors. The empirical part of the hybrid model uses Partial Least Squares (PLS) regression to estimate biomass, carbon source, and pertactin productivity from fluorescence data. In view that significant correlations are observed between oxidative stress and productivity, the mechanistic part of the hybrid model is based on key oxidative reaction pathways. Estimation based on a hybrid model is shown to improve the prediction accuracy of antigen productivity as compared to purely empirical or purely mechanistic model-based estimators. The proposed estimator enables real-time monitoring of the manufacturing process and opens the possibility of future implementation of mid-point corrective actions.

{"title":"A model based approach for monitoring Bordetella pertussis fermentation with an inline spectro-fluorescence probe","authors":"Abhishek Mishra , Michael Vitelli , Boris Tartakovsky , Ibrahim M. Tamer , Hector Budman","doi":"10.1016/j.bej.2025.109674","DOIUrl":"10.1016/j.bej.2025.109674","url":null,"abstract":"<div><div>Presently, the extraction of the antigen pertactin poses a challenge in the manufacturing of the whooping cough vaccine due to its low and variable yield [1]. In this work, a hybrid model that combines empirical and mechanistic parts and in-line fluorescence measurements is used to design an estimator for monitoring the manufacturing process in bioreactors. The empirical part of the hybrid model uses Partial Least Squares (PLS) regression to estimate biomass, carbon source, and pertactin productivity from fluorescence data. In view that significant correlations are observed between oxidative stress and productivity, the mechanistic part of the hybrid model is based on key oxidative reaction pathways. Estimation based on a hybrid model is shown to improve the prediction accuracy of antigen productivity as compared to purely empirical or purely mechanistic model-based estimators. The proposed estimator enables real-time monitoring of the manufacturing process and opens the possibility of future implementation of mid-point corrective actions.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109674"},"PeriodicalIF":3.7,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143430121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing bamboo biomass for sustainable isobutanol production using genetically engineered Escherichia coli

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-11 DOI: 10.1016/j.bej.2025.109669

Said Nawab , Zahoor , Syed Bilal Shah , Mujeeb Ur Rahman , Hareef Ahmed Keerio , Inamur Rahman

Researchers are finding sustainable fuel alternatives due to the growing costs and environmental issues related to fossil energy. Isobutanol is an attractive biofuel with a higher energy content than ethanol and the potential to replace gasoline. Bamboo emerges as a promising and cost-effective feedstock for isobutanol production due to its fast growth and high holocellulosic content. By using response surface methodology, this study optimized glucose recovery from bamboo biomass by varying pretreatment temperature (30–70°C), reaction time (0.5–6 h), and NaOH concentration (0.5–3 %). The maximum glucose recovery was achieved using 1 % NaOH at 68°C for 6 hours, resulting in improved cellulose and reduced hemicellulose and lignin content in bamboo. This process released 31.01 mg/mL glucose, representing 65 % of available sugars. Structural modifications of the untreated and alkali-treated bamboo biomass were confirmed through Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Isobutanol fermentation with the engineered E. coli strain JCL260 produced 2.28 g/L of isobutanol from enzymatic hydrolysate, achieving 22.86 % of the theoretical maximum. This study determines that cellulose from bamboo has been efficiently transformed into isobutanol through fermentation, although at a lower concentration associated with pure glucose.

{"title":"Optimizing bamboo biomass for sustainable isobutanol production using genetically engineered Escherichia coli","authors":"Said Nawab , Zahoor , Syed Bilal Shah , Mujeeb Ur Rahman , Hareef Ahmed Keerio , Inamur Rahman","doi":"10.1016/j.bej.2025.109669","DOIUrl":"10.1016/j.bej.2025.109669","url":null,"abstract":"<div><div>Researchers are finding sustainable fuel alternatives due to the growing costs and environmental issues related to fossil energy. Isobutanol is an attractive biofuel with a higher energy content than ethanol and the potential to replace gasoline. Bamboo emerges as a promising and cost-effective feedstock for isobutanol production due to its fast growth and high holocellulosic content. By using response surface methodology, this study optimized glucose recovery from bamboo biomass by varying pretreatment temperature (30–70°C), reaction time (0.5–6 h), and NaOH concentration (0.5–3 %). The maximum glucose recovery was achieved using 1 % NaOH at 68°C for 6 hours, resulting in improved cellulose and reduced hemicellulose and lignin content in bamboo. This process released 31.01 mg/mL glucose, representing 65 % of available sugars. Structural modifications of the untreated and alkali-treated bamboo biomass were confirmed through Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Isobutanol fermentation with the engineered <em>E. coli</em> strain JCL260 produced 2.28 g/L of isobutanol from enzymatic hydrolysate, achieving 22.86 % of the theoretical maximum. This study determines that cellulose from bamboo has been efficiently transformed into isobutanol through fermentation, although at a lower concentration associated with pure glucose.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109669"},"PeriodicalIF":3.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0