Biochemical Engineering Journal最新文献_第5页

Volemitol production from Yarrowia lipolytica via transaldolase gene (TAL) disruption and erythrose-4-phosphate (E4P) flux regulation 通过反式脱醛酶基因 (TAL) 干扰和赤藓糖-4-磷酸 (E4P) 通量调节从脂肪分解酵母菌中产生油醇

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-23 DOI: 10.1016/j.bej.2024.109535

Liyun Ji, Qing Li, Ye Li, Shuo Xu, Hairong Cheng

Volemitol (D-glycero-D-manno-Heptitol, C₇H₁₆O₇ with CAS No.488–38–0), a seven-carbon (C7) sugar-derived alcohol, has the potential to be used as a natural sweetener. The natural scarcity of sugar alcohols restricts their practical uses. However, Yarrowia lipolytica has shown significant promise in industrial production due to its capability to efficiently produce sugar alcohols like erythritol, D-threitol, mannitol, and xylitol by generating key biosynthetic intermediates through glycolysis and the phosphopentose (PPP) pathway. In this study, the transaldolase gene (TAL) in the erythritol synthesis pathway was knocked out in the erythritol-producing Y. lipolytica strain CGMCC7326 to inhibit erythritol production. TAL-deleted strain Y. lipolytica CGMCC7326ΔTAL exhibited a notable decline in erythritol production; however, a novel substance, volemitol, was generated from glucose at a titer of 50 g/L. Volemitol with 99 % purity was obtained as a white microneedle powder crystal through the enzymatic activity of mannitol dehydrogenase (MDH2), which reduces sedoheptulose to volemitol. The proposed biosynthetic pathway in Y. lipolytica CGMCC7326ΔTAL is: sedoheptulose-7-phosphate was converted to sedoheptulose, then was reduced to volemitol. In conclusion, this study is the first to report efficient volemitol production from glucose via fermentation by engineered Y. lipolytica.

沃尔米醇（D-甘油-D-甘露庚醇，C7H16O7，化学文摘社编号：488-38-0）是一种七碳（C7）糖醇，具有作为天然甜味剂的潜力。糖醇的天然稀缺性限制了其实际用途。然而，脂肪分解亚罗菌（Yarrowia lipolytica）通过糖酵解和磷酸戊糖（PPP）途径产生关键的生物合成中间体，能够高效地生产赤藓糖醇、D-苏力糖醇、甘露糖醇和木糖醇等糖醇，因而在工业生产中显示出巨大的前景。本研究敲除了生产赤藓糖醇的脂溶性酵母菌株 CGMCC7326 中赤藓糖醇合成途径中的反醛醇酶基因（TAL），以抑制赤藓糖醇的生产。TAL缺失菌株 Y. lipolytica CGMCC7326ΔTAL 的赤藓糖醇产量明显下降，但却以 50 克/升的滴度从葡萄糖中生成了一种新物质--伏来米醇。通过甘露醇脱氢酶（MDH2）的酶活性，将沉淀七聚糖还原为油菜醇，从而获得纯度为 99% 的白色微针粉末晶体。所提出的 Y. lipolytica CGMCC7326ΔTAL 的生物合成途径是：7-磷酸沉降色酮糖被转化为沉降色酮糖，然后被还原为 volemitol。总之，本研究首次报道了工程脂溶性酵母通过发酵从葡萄糖中高效生产出伏来米醇。

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引用次数: 0

Enhanced antimicrobial performance of single-use polyamide 56 nanofiber membranes modified with chitosan, reactive dyes, and poly(hexamethylene biguanide) (PHMB) for biological waste treatments 用壳聚糖、活性染料和聚六亚甲基双胍 (PHMB) 改性的一次性聚酰胺 56 纳米纤维膜的抗菌性能增强，可用于生物废物处理

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-22 DOI: 10.1016/j.bej.2024.109538

Jia-Hong Yang , Quang-Vinh Le , Bing-Lan Liu , Penjit Srinophakun , Chi-Yun Wang , Chen‑Yaw Chiu , I-Son Ng , Kuei-Hsiang Chen , Yu-Kaung Chang

Polyamide 56 (PA56), derived from renewable resources like wheat and corn via microbial fermentation, presents a sustainable alternative to traditional petrochemical-based polymers and has gained attention for its versatility in various fields. This study explores the antibacterial properties of bio-nylon nanofibers through fabrication and modification processes. PA56 nanofiber membrane was initially fabricated using electrospinning technology. These membranes were then modified with chitosan (CS), forming PA56-CS membranes, and further enhanced with Reactive dyes (Reactive Green 19, RG19 and Reactive Red 141, RR141) to produce PA56-CS-DYE membranes. Poly(hexamethylene biguanide) (PHMB), a positively charged polymer, was subsequently bonded to these membranes, resulting in PA56-CS-DYE-PHMB nanofiber membranes. Comprehensive physical characterizations using Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermogravimetric analysis (TGA) were conducted alongside qualitative and quantitative antibacterial assays against E. coli. The PA56-CS-RR141-PHMB nanofiber membrane exhibited exceptional antibacterial efficiency (AE), exceeding 97 %, indicating strong potential for biological waste treatment and related applications. However, the high antibacterial performance of these membranes is primarily suited for single-use systems, as their efficacy diminishes with repeated use, making them ideal for immediate and short-term applications in healthcare and environmental settings.

聚酰胺 56（PA56）通过微生物发酵从小麦和玉米等可再生资源中提取，是传统石化基聚合物的可持续替代品，因其在各个领域的多功能性而备受关注。本研究通过制备和改性工艺探索了生物尼龙纳米纤维的抗菌特性。PA56 纳米纤维膜最初采用电纺丝技术制造。然后用壳聚糖（CS）对这些膜进行改性，形成 PA56-CS 膜，再用活性染料（活性绿 19、RG19 和活性红 141、RR141）进一步增强，制成 PA56-CS-DYE 膜。带正电荷的聚合物聚六亚甲基双胍（PHMB）随后被粘合到这些膜上，形成 PA56-CS-DYE-PHMB 纳米纤维膜。利用傅立叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和热重分析（TGA）进行了全面的物理表征，并对大肠杆菌进行了定性和定量的抗菌检测。PA56-CS-RR141-PHMB 纳米纤维膜表现出卓越的抗菌效率（AE），超过 97%，显示出其在生物废物处理和相关应用方面的巨大潜力。不过，这些膜的高抗菌性能主要适用于一次性使用系统，因为它们的功效会随着重复使用而降低，因此非常适合医疗保健和环境领域的即时和短期应用。

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引用次数: 0

Recent trends in metabolic engineering for microbial production of value-added natural products 微生物生产增值天然产品的代谢工程最新趋势

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-21 DOI: 10.1016/j.bej.2024.109537

Prasenjit Chakraborty , Randhir Kumar , Sanjay Karn , Payal Patel , Haren Gosai

Microbe-mediated chemical production is a sustainable approach for production of natural products that are important for our society. Traditional fermentation production of natural products viz. drugs, nutraceuticals, pigments, aromatic compounds, and biofuels has limitations such as low yield, high cost and time- consuming process. Significant success in the economical production of these natural products has been established by altering the metabolic pathways of microorganisms through metabolic engineering. Recent advancements in new tools and strategies have led to a significant reduction in the turnaround time in the classic approach i.e. design-build-test-learn (DBTL) cycle in metabolic engineering. Here, we summarize various tools and strategies developed in the area of metabolic engineering with a focus on value-added natural products Also, this review lists bioprospecting of microorganisms through metabolic engineering in last five years for production of natural products. At the end, outlook, challenges and opportunities for the successful establishment of robust engineered microbial cell factories have been reported.

以微生物为媒介的化学生产是一种生产对社会十分重要的天然产品的可持续方法。药物、营养保健品、色素、芳香化合物和生物燃料等天然产品的传统发酵生产具有产量低、成本高和耗时长等局限性。通过代谢工程改变微生物的代谢途径，在经济生产这些天然产品方面取得了巨大成功。新工具和新策略的最新进展大大缩短了传统方法（即代谢工程中的设计-构建-测试-学习（DBTL）循环）的周转时间。在此，我们总结了代谢工程领域开发的各种工具和策略，重点关注增值天然产品。此外，本综述还列举了过去五年中通过代谢工程对微生物进行生物勘探以生产天然产品的情况。最后，报告了成功建立强大的工程微生物细胞工厂的前景、挑战和机遇。

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引用次数: 0

Assembly of a tumor microtissue by stacking normal and cancer spheroids on Kenzan using a bio-3D printer to monitor dynamic cancer cell invasion in the microtissue 使用生物三维打印机将正常球体和癌症球体堆叠在肯赞上，组装成肿瘤微组织，以监测微组织中癌细胞的动态侵袭情况

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-19 DOI: 10.1016/j.bej.2024.109536

Kazuaki Ninomiya , Tatsuhiko Taniuchi

In the present study, a tumor microtissue was assembled by precisely stacking normal and cancer cell spheroids on Kenzan (microneedle array) using a spheroid stacking type bio-3D printer. This is the first study to non-invasively observe the dynamic behavior of GFP-tagged cancer cell invasion in the microtissue assembled by a spheroid stacking type bio-3D printer. First, the cancer cell spheroid was prepared using 10 % cancer cells (MCF-7 expressing GFP) and 90 % normal cells (70 % HNDF and 20 % HUVEC). The normal cell spheroid was prepared using 100 % normal cells (80 % HNDF and 20 % HUVEC). The tumor microtissue was then assembled on Kenzan by placing 1 cancer cell spheroid in the center position of the microtissue and 8 normal cell spheroids around it. 9 spheroids stacked on Kenzan were fused into 1 tumor microtissue after 24 hours of culture. The green fluorescence derived from cancer cells spread from the central position to the entire area of the tumor microtissue. The spread dynamics of cancer cell-derived GFP fluorescence can be used as a simple measure to evaluate cancer cell migration/invasion and response to anticancer drugs.

在本研究中，利用球形堆叠式生物三维打印机将正常细胞球体和癌细胞球体精确堆叠在 Kenzan（微针阵列）上，组装成肿瘤微组织。这是首次利用球形堆叠式生物三维打印机无创观察 GFP 标记的癌细胞侵袭微组织的动态行为。首先，用 10% 的癌细胞（表达 GFP 的 MCF-7）和 90% 的正常细胞（70% 的 HNDF 和 20% 的 HUVEC）制备癌细胞球。正常细胞球使用 100 % 的正常细胞（80 % HNDF 和 20 % HUVEC）制备。然后在 Kenzan 上组装肿瘤微组织，在微组织中心位置放置 1 个癌细胞球体，周围放置 8 个正常细胞球体。堆叠在 Kenzan 上的 9 个球体在培养 24 小时后融合成 1 个肿瘤微组织。癌细胞发出的绿色荧光从中心位置扩散到肿瘤微组织的整个区域。癌细胞衍生的 GFP 荧光的扩散动态可作为评估癌细胞迁移/侵袭和对抗癌药物反应的一种简单方法。

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引用次数: 0

Adsorption characteristics of virus-mimetic fluorescent nanoparticles on polymer fiber material surfaces 仿病毒荧光纳米粒子在聚合物纤维材料表面的吸附特性

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-19 DOI: 10.1016/j.bej.2024.109534

Rina Uchida , Ayuri Mitsuno , Tomohiro Komatsu , Chisato Sakamoto , Satoshi Amaya , Satoshi Migita , Eiichiro Takamura , Hiroaki Sakamoto

Contact infection, whereby the virus is transmitted through objects to which it adheres, affects the spread of infection. Although inactivation with alcohol is commonly used, the virus may adhere to the surface again after removal. It is necessary to take measures against direct and contact infection to control the spread of viral infection. However, the type of material the viruses adsorb onto is not fully understood. The objective in the present study is to elucidate virus adsorption behavior to suppress the indirect spread of infection. We examined for materials that are difficult for viruses to adhere to and investigated their adsorption mechanisms to control the spread of infection through contact infection. In this study, spike protein-modified fluorescent nanoparticles (SFNs) were designed and fabricated by modifying the surface of fluorescent particles with the spike S1 protein, which is expected to first contact and adsorb onto the material surface. Purpose of the present study is to analyze the influence of material characteristics on virus adhesion using SFNs. SFNs were adsorbed onto Nylon, polyester (PET), polypropylene (PP), polytetrafluoroethylene (PTFE), acrylic and rayon, and their fluorescence intensities and adsorption characteristics were compared by scanning electron microscopy (SEM) analysis and fluorescence analysis. Principal component analysis showed that virus adsorption was more sensitive to coarseness for PTFE than for other fibers.

接触传染，即病毒通过其附着的物体传播，会影响感染的扩散。虽然常用酒精灭活，但清除后病毒可能会再次附着在物体表面。有必要采取措施防止直接感染和接触感染，以控制病毒感染的传播。然而，人们对病毒吸附在哪种材料上并不完全了解。本研究的目的是阐明病毒的吸附行为，以抑制感染的间接传播。我们研究了病毒难以吸附的材料，并调查了它们的吸附机制，以控制通过接触感染的传播。本研究设计并制造了尖峰蛋白修饰的荧光纳米粒子（SFNs），方法是用尖峰 S1 蛋白修饰荧光粒子表面，使其首先接触并吸附在材料表面。本研究的目的是利用 SFNs 分析材料特性对病毒吸附的影响。研究人员将 SFNs 吸附在尼龙、聚酯（PET）、聚丙烯（PP）、聚四氟乙烯（PTFE）、腈纶和人造丝上，并通过扫描电子显微镜（SEM）分析和荧光分析比较了它们的荧光强度和吸附特性。主成分分析表明，与其他纤维相比，聚四氟乙烯纤维的病毒吸附对粗细度更为敏感。

{"title":"Adsorption characteristics of virus-mimetic fluorescent nanoparticles on polymer fiber material surfaces","authors":"Rina Uchida , Ayuri Mitsuno , Tomohiro Komatsu , Chisato Sakamoto , Satoshi Amaya , Satoshi Migita , Eiichiro Takamura , Hiroaki Sakamoto","doi":"10.1016/j.bej.2024.109534","DOIUrl":"10.1016/j.bej.2024.109534","url":null,"abstract":"<div><div>Contact infection, whereby the virus is transmitted through objects to which it adheres, affects the spread of infection. Although inactivation with alcohol is commonly used, the virus may adhere to the surface again after removal. It is necessary to take measures against direct and contact infection to control the spread of viral infection. However, the type of material the viruses adsorb onto is not fully understood. The objective in the present study is to elucidate virus adsorption behavior to suppress the indirect spread of infection. We examined for materials that are difficult for viruses to adhere to and investigated their adsorption mechanisms to control the spread of infection through contact infection. In this study, spike protein-modified fluorescent nanoparticles (SFNs) were designed and fabricated by modifying the surface of fluorescent particles with the spike S1 protein, which is expected to first contact and adsorb onto the material surface. Purpose of the present study is to analyze the influence of material characteristics on virus adhesion using SFNs. SFNs were adsorbed onto Nylon, polyester (PET), polypropylene (PP), polytetrafluoroethylene (PTFE), acrylic and rayon, and their fluorescence intensities and adsorption characteristics were compared by scanning electron microscopy (SEM) analysis and fluorescence analysis. Principal component analysis showed that virus adsorption was more sensitive to coarseness for PTFE than for other fibers.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109534"},"PeriodicalIF":3.7,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Process modeling and 3-stage photobioreactor design for algae cultivation and CO2 capture: A case study using palm oil mill effluent 用于藻类培养和二氧化碳捕获的工艺建模和三阶段光生物反应器设计：利用棕榈油厂废水的案例研究

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-18 DOI: 10.1016/j.bej.2024.109532

Emmanuel Yahaya , Wan Sieng Yeo , Jobrun Nandong

Microalgae have advantages, including rapid growth rates, a high lipid production capacity, effective removal of nitrates and phosphates from wastewater, and efficient carbon dioxide (CO₂) absorption. The optimal operating conditions and strategies of microalgae cultivation can vary significantly from one goal to another. An economic approach to exploring various operating strategies is doable via microalgal process modeling and simulation. Therefore, this study aims to develop a simulation model aimed at enhancing algae growth within a photobioreactor (PBR) system designed to reduce CO₂ emissions in palm oil mills. This simulation model is constructed to explore the algae growth CO₂ capture efficiency and the influence of oxygen (O₂) in the water in the PBR. This study achieved a CO₂ capture efficiency of up to 60 % which represents the highest capture, and a dissolved O₂ of 20 % was achieved due to the effect of the mass transfer coefficient. Algal growth exhibited a high rate, approximately 1057 g/m³, which could serve as a potential pathway for biodiesel or biobutanol production. Additionally, this study underscores the significant role of the mass transfer coefficient in effectively reducing liquid O₂ levels to maximize CO₂ capture and achieve a high algae yield. Furthermore, the simulation results reveal that a high concentration of O₂ in the water promotes photorespiration, which hampers algal growth and reduces CO₂ capture efficiency.

微藻具有生长速度快、产脂能力强、能有效去除废水中的硝酸盐和磷酸盐以及高效吸收二氧化碳（CO2）等优点。微藻培养的最佳操作条件和策略会因目标不同而有很大差异。通过微藻工艺建模和模拟，可以采用经济的方法来探索各种操作策略。因此，本研究旨在开发一个仿真模型，以提高光生物反应器（PBR）系统中的藻类生长，从而减少棕榈油厂的二氧化碳排放。该模拟模型旨在探索藻类生长的二氧化碳捕获效率以及光生物反应器中水中氧气（O2）的影响。这项研究实现了高达 60% 的二氧化碳捕获效率，这代表了最高的捕获效率，由于传质系数的影响，实现了 20% 的氧气溶解度。藻类的生长率很高，约为 1057 克/立方米，可作为生产生物柴油或生物丁醇的潜在途径。此外，这项研究还强调了传质系数在有效降低液态氧气水平以最大限度地捕获二氧化碳和实现高产藻类方面的重要作用。此外，模拟结果表明，水中高浓度的氧气会促进光呼吸，从而阻碍藻类生长并降低二氧化碳捕获效率。

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引用次数: 0

Enhanced catalytic activity and reusability of sucrose phosphorylase@magnetic nanoparticles by surface-coating amorphous ZIF-67 通过表面包覆无定形 ZIF-67 增强蔗糖磷酸化酶@磁性纳米粒子的催化活性和重复使用性

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-16 DOI: 10.1016/j.bej.2024.109533

Zhendong Liu , Haichang Xu , Bin Wei , Hao Liang

Sucrose phosphorylase (SPase) is a highly efficient glycosyltransferase which has a wide range of substrate specificity and excellent application prospects in the cosmetics, food, and medicine fields. The application of free SPase is limited due to its high cost, poor stability and poor reusability. Immobilization of enzymes can solve these problems. In this study, SPase was firstly immobilized by magnetic nanoparticles (MNPs) and surface-coating amorphous ZIF-67 for enhancing catalytical activity and reusability. ZIF-67@SPase@MNPs with MNPs core and ZIF-67 shell was characterized by TEM, SEM, XRD, FT-IR and VSM. Compared with free SPase, the catalytic activity of ZIF-67@SPase@MNPs increased by 30 %. Coated with amorphous ZIF-67, the immobilized enzyme retained 70 % relative activity after 12 cycles and 80 % relative activity after 15 days of storage. In addition, ZIF-67@SPase@MNPs had strong magnetic properties and the saturation magnetization was 52.07 emu/g. Surface-coating amorphous ZIF-67 on SPase@MNPs is a promising method for immobilizing enzymes, which can improve catalytic activity and reusability, therefore showing great application potential in biocatalysts and product separation. It has a good application prospect in the production of high viscosity glycosides.

蔗糖磷酸化酶（SPase）是一种高效的糖基转移酶，具有广泛的底物特异性，在化妆品、食品和医药领域具有良好的应用前景。由于游离 SPase 成本高、稳定性差、重复利用率低，其应用受到限制。酶的固定化可以解决这些问题。本研究首先利用磁性纳米颗粒（MNPs）固定化 SPase，然后在其表面包覆无定形 ZIF-67，以提高其催化活性和可重复使用性。研究人员利用 TEM、SEM、XRD、FT-IR 和 VSM 对以 MNPs 为核心、ZIF-67 为外壳的 ZIF-67@SPase@MNPs 进行了表征。与游离 SPase 相比，ZIF-67@SPase@MNPs 的催化活性提高了 30%。包覆了无定形 ZIF-67 的固定化酶在 12 个循环后保持了 70% 的相对活性，在储存 15 天后保持了 80% 的相对活性。此外，ZIF-67@SPase@MNPs 还具有很强的磁性，其饱和磁化率为 52.07 emu/g。在 SPase@MNPs 表面包覆无定形 ZIF-67 是一种很有前景的固定化酶的方法，可以提高催化活性和重复利用率，因此在生物催化剂和产物分离方面具有很大的应用潜力。它在高粘度糖苷的生产中具有良好的应用前景。

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引用次数: 0

Removal of selenate from wastewater using a bioelectrochemical reactor: The importance of measuring selenide and the role of competing anions 利用生物电化学反应器去除废水中的硒酸盐：测量硒化物的重要性和竞争阴离子的作用

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-15 DOI: 10.1016/j.bej.2024.109531

Benhur K. Asefaw , Huan Chen , Youneng Tang

Removal of selenate (SeO₄^2-) from selenate-contaminated wastewater is challenging due to the commonly co-existing and competing anions of sulfate (SO₄^2-) and nitrate (NO₃^-). This study investigates SeO₄²⁻ reduction to elemental selenium (Se⁰) in a cathode-based bioelectrochemical (BEC) reactor and a conventional biofilm reactor (i.e., an upflow anaerobic reactor). The simulated wastewater contained SeO₄²⁻ at a typical concentration of 5 mg Se/L, SO₄²⁻ at a typical concentration of 1000 mg S/L, and NO₃⁻ at concentrations that varied from 0 to 10 mg N/L. The impact of sulfate on the BEC reactor was much lower than that on the conventional reactor: The selenium removal, defined as (selenate in influent – dissolved selenium in effluent)/selenate in influent, was 99 % in the BEC reactor versus 69 % in the conventional biofilm reactor. The lower selenium removal in the conventional reactor was mainly due to the >10 times higher reduction of sulfate, which directly caused competition between sulfate and selenate for the common resources such as electrons. The more reduction of sulfate in the conventional reactor further led to 45 times higher production of selenide. Selenide is usually assumed to be minimal and therefore not measured in the literature. This simplification may significantly overestimate selenium removal when the influent sulfate concentration is very high. NO₃^- in the influent of the BEC reactor promoted selenium removal when it was less than 5.0 mg N/L but inhibited selenate removal when it was more than 7.5 mg N/L. This was supported by the microbial community analysis and intermediate (nitrite) analysis.

由于硫酸盐（SO42-）和硝酸盐（NO3-）这两种阴离子通常同时存在并相互竞争，因此从硒酸盐污染的废水中去除硒酸盐（SeO42-）具有挑战性。本研究调查了在阴极生物电化学（BEC）反应器和传统生物膜反应器（即上流式厌氧反应器）中将 SeO42- 还原为元素硒（Se0）的情况。模拟废水中 SeO42- 的典型浓度为 5 毫克 Se/升，SO42- 的典型浓度为 1000 毫克 S/升，NO3- 的浓度为 0 至 10 毫克 N/升。硫酸盐对 BEC 反应器的影响远远低于对传统反应器的影响：BEC 反应器的硒去除率为 99%（进水硒酸盐-出水溶解硒）/进水硒酸盐，而传统生物膜反应器的硒去除率为 69%。传统反应器中硒去除率较低的主要原因是硫酸盐的还原率高达 10 倍，这直接导致了硫酸盐和硒酸盐对电子等共同资源的竞争。传统反应器中更多的硫酸盐还原进一步导致硒化物产量增加 45 倍。硒化物通常被假定为极少量，因此文献中没有对其进行测量。当进水硫酸盐浓度很高时，这种简化可能会大大高估硒的去除率。当 BEC 反应器进水中的 NO3- 小于 5.0 毫克 N/L 时，会促进硒的去除，但当 NO3- 大于 7.5 毫克 N/L 时，则会抑制硒的去除。微生物群落分析和中间产物（亚硝酸盐）分析也证实了这一点。

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引用次数: 0

Corn stover waste preparation cerium-modified biochar for phosphate removal from pig farm wastewater: Adsorption performance and mechanism 玉米秸秆废料制备的铈改性生物炭用于去除养猪场废水中的磷酸盐：吸附性能和机理

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-13 DOI: 10.1016/j.bej.2024.109530

Liu Lou , Weikun Li , Hao Yao , Huiying Luo , Gang Liu , Jun Fang

The high phosphorus content in livestock and poultry wastewater is a significant factor contributing to water eutrophication. It is imperative to seek an economically efficient method for phosphate recovery. This study employed cerium-modified biochar to recover phosphate from pig farm wastewater. An investigation was conducted to examine the adsorption performance and removal mechanism of phosphate. Among the different samples, 0.1CeB-500℃ was selected for subsequent experiments. It exhibited a phosphate adsorption capacity of 9.58 mg/g and a removal efficiency of 95.75 %. The results showed that the phosphate adsorption process followed not only the pseudo-second-order kinetic model, but also the Langmuir isotherm model. It suggested that the adsorption of phosphate onto the biochar occurred in a monolayer chemical manner, with a maximum adsorption capacity of 10.86 mg/g. Phosphate adsorption was minimally affected within the pH range of 2–9, with Cl^- having negligible impact, NO₃^- slightly inhibiting, and HCO₃^- and CO₃^2- significantly hindering phosphate adsorption. A series of characterization results indicated that phosphate removal occurred through surface precipitation forming CePO₄, ligand exchange between carbonate and phosphate, inner-sphere complexation, and electrostatic attraction. The phosphate removal efficiency from pig farm wastewater was 43.25 %, demonstrating promising potential for practical application.

畜禽废水中的高磷含量是导致水体富营养化的一个重要因素。当务之急是寻求一种经济高效的磷酸盐回收方法。本研究采用铈改性生物炭从养猪场废水中回收磷酸盐。对磷酸盐的吸附性能和去除机理进行了研究。在不同的样品中，选择了 0.1CeB-500℃ 进行后续实验。其磷酸盐吸附容量为 9.58 mg/g，去除率为 95.75 %。结果表明，磷酸盐的吸附过程不仅遵循伪二阶动力学模型，而且遵循 Langmuir 等温线模型。结果表明，生物炭对磷酸盐的吸附是以单层化学方式进行的，最大吸附容量为 10.86 mg/g。在 pH 值为 2-9 的范围内，磷酸盐的吸附受到的影响很小，Cl- 的影响可以忽略不计，NO3- 有轻微的抑制作用，而 HCO3- 和 CO32- 则明显阻碍磷酸盐的吸附。一系列表征结果表明，磷酸盐的去除是通过表面沉淀形成 CePO4、碳酸盐与磷酸盐之间的配体交换、内球络合和静电引力实现的。养猪场废水中的磷酸盐去除率为 43.25%，显示出了很好的实际应用潜力。

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引用次数: 0

Power production characteristics of binary particles pulsed anaerobic fluidized bed microbial fuel cell 二元颗粒脉冲厌氧流化床微生物燃料电池的发电特性

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-11 DOI: 10.1016/j.bej.2024.109524

Yangfan Song, Meng Wang, Yiming Han, Yanmin Li, Hongwei Chen, Xiang Wei, Zhuo Liu

A novel binary particulate pulsating anaerobic fluidized bed microbial fuel cell (BPFB-MFC) was designed and constructed in order to improve the efficiency of low-grade energy conversion in sewage. The effects of pulsed liquid flow rate and bed filling rate on the electricity production performance and effluent treatment characteristics of the BPFB-MFC were investigated experimentally. The results showed that when the pulsed liquid flow was u=1.95sin(π/3)t cm·s⁻¹ and when the bed materials in the anode chamber consisted of 10 % bed height activated carbon particles and 10 % bed height ceramic particles, the highest voltage produced was 519.7 mV, the highest power density was 587.5 mW·m⁻², and the lowest internal resistance was 171.2Ω, which was the optimal experimental working condition. It was found that the electricity production performance and effluent treatment efficiency of the mixed particles system were better than those of a system with single particles. This work held promise of promoting the industrialization of MFC.

为了提高污水中低品位能量的转化效率，设计并构建了一种新型二元颗粒脉动厌氧流化床微生物燃料电池（BPFB-MFC）。实验研究了脉冲液体流速和床层填充率对 BPFB-MFC 发电性能和污水处理特性的影响。结果表明，当脉冲液流为 u=1.95sin(π/3)t cm-s-1，阳极室的床层材料由 10 % 的床层高度活性炭颗粒和 10 % 的床层高度陶瓷颗粒组成时，产生的最高电压为 519.7 mV，最高功率密度为 587.5 mW-m-2，最低内阻为 171.2Ω，这是最佳的实验工作条件。实验发现，混合颗粒系统的发电性能和污水处理效率均优于单一颗粒系统。这项工作有望促进 MFC 的产业化。

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