Biochemical Engineering Journal最新文献_第5页

Amelioration of mining-area soils with microbial induction of magnesium ammonium phosphate 微生物诱导磷酸铵镁改良矿区土壤

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-04-01 Epub Date: 2026-01-07 DOI: 10.1016/j.bej.2026.110075

Zhe Wang , Wen-yan Zhang , Jun-li Cheng , Shu-hui Wei , Hong-yuan Cui , Wei-da Wang

Large-scale mineral exploitation causes heavy metal pollution and nutrient depletion in mining-area soils, making heavy metal control and soil fertility improvement urgent. This study synthesized biological magnesium ammonium phosphate (bio-MAP) via microbially induced mineralization, characterized its microstructure, and conducted pot experiments with ryegrass and alfalfa under different bio-MAP additions to analyze effects on soil physicochemical properties, Pb²⁺/Cd²⁺ contents, and plant growth. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) confirmed the precipitate was mainly struvite (MgNH₄PO₄·6H₂O) with a regular orthorhombic structure. Appropriate bio-MAP addition not only facilitated the formation of large soil aggregates and enhanced soil structural stability but also significantly promoted the growth of ryegrass and alfalfa. At the 30-day harvest, the total fresh weight of ryegrass reached 1.33 g in the P5 treatment (bio-MAP addition: 1000 mg/kg), while that of alfalfa reached 2.36 g in the P3 treatment (bio-MAP addition: 500 mg/kg). Compared with the control, the P1, P3, and P5 treatments reduced soil available Pb content by 7.16 %, 15.1 %, and 19.28 %, respectively, and soil available Cd content by 58.57 %, 62.31 %, and 65.1 %, respectively. Bio-MAP also increased soil sucrase activity and abundances of Proteobacteria, Actinobacteria, and Bacillus. This study provides a new method for mine soil amendment and heavy metal control, plus references for ecological restoration.

大规模矿产开采造成矿区土壤重金属污染和养分耗竭，治理重金属和提高土壤肥力刻不容缓。本研究通过微生物诱导矿化法合成了生物磷酸镁铵（bio-MAP），对其微观结构进行了表征，并在不同生物map添加量的黑麦草和苜蓿上进行了盆栽实验，分析了生物map添加量对土壤理化性质、Pb 2 + /Cd 2 +含量以及植物生长的影响。x射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和扫描电镜（SEM）证实沉淀主要为鸟粪石（MgNH₄PO₄·6H₂O），具有规则的正交结构。适当添加生物map不仅有利于土壤团聚体的形成，增强土壤结构稳定性，而且显著促进黑麦草和苜蓿的生长。收获30 d时，P5处理（bio-MAP添加量为1000 mg/kg）黑麦草总鲜重达到1.33 g， P3处理（bio-MAP添加量为500 mg/kg）紫花苜蓿总鲜重达到2.36 g。与对照相比，P1、P3和P5处理土壤有效Pb含量分别降低了7.16 %、15.1 %和19.28 %，土壤有效Cd含量分别降低了58.57 %、62.31 %和65.1% %。Bio-MAP还增加了土壤蔗糖酶活性和变形菌、放线菌和芽孢杆菌的丰度。该研究为矿山土壤修复和重金属控制提供了新的方法，并为生态修复提供了参考。

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引用次数: 0

Transient heat and mass transfer in a wall-cooled packed-tray bioreactor for solid-state fermentation: An engineering framework for parameter estimation 固态发酵用壁冷填料托盘生物反应器的瞬态传热传质：参数估计的工程框架

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-04-01 Epub Date: 2026-01-03 DOI: 10.1016/j.bej.2026.110071

Gerardo A. Gómez-Ramos , Lilia A. Prado-Barragán , Sergio Huerta-Ochoa , Victor M. Rivera , Ángeles García-Hernández , Carlos O. Castillo-Araiza

Solid-state fermentation (SSF) of agrifood byproducts (AFBP) offers a sustainable route for producing high-value biomolecules, yet its industrial adoption is constrained by limited understanding of transport phenomena in wall-cooled packed bioreactors. Despite their potential for large-scale SSF, reliable methods for describing heat and mass transfer remain scarce. Building on previous fluid-dynamic studies, this work characterizes intrinsic transport mechanisms in a bench-scale wall-cooled tray packed with AFBP as substrate. An engineering framework not previously applied to these systems, integrating abiotic experiments, fluid dynamics, and transient analysis, was applied to estimate the effective thermal conductivity (

k_{eff}

), wall heat transfer coefficient (

h_{w}

), and axial dispersion coefficient (

D_{ax}

). Experiments under conditions favorable to Yarrowia lipolytica 2.2ab (318.15 K, 60 % moisture on a dry-weight basis, and inlet specific airflow rates, VkgM, of 1.875–6.250 L.kg⁻¹.min⁻¹) yielded values of 0.565–4.34 W.m⁻¹ .K⁻¹ for

k_{eff}

, 0.865–25.9 W.m⁻².K⁻¹ for

h_{w}

, and 2.60 × 10⁻³ –5.30 × 10⁻³ m².s⁻¹ for

D_{ax}

. These results differ from previous SSF studies employing AFBP, highlighting the influence of reactor configuration, operating conditions, and parameter estimation approach. The proposed methodology provides a reliable framework for evaluating heat and mass transfer in SSF systems using AFBP as a substrate and establishes a foundation for predictive design and scale-up of packed bioreactors in biotechnological applications.

农业食品副产品（AFBP）的固态发酵（SSF）为生产高价值生物分子提供了一条可持续的途径，但其工业应用受到对壁冷填充生物反应器中运输现象的有限理解的限制。尽管它们具有大规模SSF的潜力，但描述传热和传质的可靠方法仍然很少。在先前的流体动力学研究的基础上，本研究表征了以AFBP为衬底的台式壁冷托盘中的固有输运机制。一个以前没有应用于这些系统的工程框架，整合了非生物实验、流体动力学和瞬态分析，用于估计有效导热系数（keff）、壁面传热系数（hw）和轴向分散系数（Dax）。实验条件为脂肪耶氏菌2.2ab (318.15 K)， 60%的水分（干重基础），入口比气流速率（VkgM）为1.875-6.250 L.kg⁻¹。w - m（⁻）的值为0.565-4.34。K⁻¹for keff, 0.865-25.9 w m⁻²。K⁻¹，以及2.60 × 10⁻³-5.30 × 10⁻³²。（对Dax来说）这些结果与先前使用AFBP的SSF研究不同，突出了反应器配置、操作条件和参数估计方法的影响。所提出的方法为评估使用AFBP作为基质的SSF系统中的传热传质提供了可靠的框架，并为生物技术应用中填料生物反应器的预测设计和放大奠定了基础。

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引用次数: 0

Competitive and synergistic effects of manganese-nitrogen co-doped sludge biochar on the chlortetracycline hydrochloride and Cu²⁺ from water 锰氮共掺杂污泥生物炭对盐酸氯四环素和水中Cu 2 +的竞争和协同效应

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-04-01 Epub Date: 2026-01-15 DOI: 10.1016/j.bej.2026.110082

Xinhua Zhou, Zhihao Li, Ziqiang Ding, Lin Lu, Letong Wang

Addressing the issue of simultaneous antibiotic and heavy metal contamination in effluent from intensive animal husbandry, this study used sewage sludge as the raw material. It employed co-doping modification with urea and manganese chloride to synthesize a sludge biochar composite (Mn-N-SBC) through pyrolysis for removing CTC and Cu^2 + from aquaculture effluent. Experimental results showed that in the single system, the Qemax values were 93.27 mg/g and 31.45 mg/g, respectively. In the binary system, the Qemax values were 94.89 mg/g and 30.76 mg/g, respectively. These findings indicate that competing interactions and synergistic effects coexist during the concurrent removal of CTC and Cu²⁺ by Mn-N-SBC. Microstructural analysis suggests that the competitive adsorption of CTC and Cu²⁺ on Mn-N-SBC mainly results from their competition for the same active sites. This enhanced adsorption can be attributed to electrostatic interactions, Cu²⁺ bridging effects, and interactions involving O/N functional groups within CTC molecules. Overall, this study clarifies the concurrent adsorption mechanism of Mn-N-SBC for CTC and Cu²⁺, offering both a strategy for sludge valorization and a template for designing multifunctional adsorbents to treat complex wastewater.

针对集约化畜牧业废水中同时存在抗生素和重金属污染的问题，本研究以污水污泥为原料。采用尿素和氯化锰共掺杂改性，热解合成污泥生物炭复合材料（Mn-N-SBC），用于去除水产养殖出水中的CTC和Cu2 +。实验结果表明，在单一体系中，Qemax值分别为93.27 mg/g和31.45 mg/g。在二元体系中，Qemax值分别为94.89 mg/g和30.76 mg/g。这些结果表明，在Mn-N-SBC同时去除CTC和Cu2+的过程中，竞争相互作用和协同效应并存。微观结构分析表明，CTC和Cu2+在Mn-N-SBC上的竞争吸附主要是由于它们对相同活性位点的竞争。这种增强的吸附可归因于静电相互作用、Cu2+桥接效应和CTC分子内O/N官能团的相互作用。总的来说，本研究阐明了Mn-N-SBC对CTC和Cu2+的同时吸附机理，为污泥增值策略和设计多功能吸附剂处理复杂废水提供了模板。

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引用次数: 0

Sustainable valorization of rapeseed meal to bioactive and nutritional products via solid-state fermentation with protease-enhanced Streptomyces sp. SCUT-3 利用蛋白酶增强链霉菌SCUT-3固态发酵将菜籽粕持续发酵为具有生物活性和营养价值的产品

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-04-01 Epub Date: 2026-01-19 DOI: 10.1016/j.bej.2026.110086

Zhen-Jie Dai , Wen-Jing Feng , Can Qin , Hua-Hong Cai , Jing-Yi Hu , Zhi-Wei Li , Ming-Shu Zhang , Lei Wu , Jun-Jin Deng , Xiao-Chun Luo

To meet the growing global demand for protein driven by population increases, converting agricultural by-products into edible protein is critical for sustainable food security. This study introduces a solid-state fermentation (SSF) platform utilizing protease-engineered Streptomyces sp. SCUT-3 for the valorization of rapeseed meal (RSM), the world’s second-largest oilseed residue. Optimized SSF parameters (substrate-liquid ratio 1:2 w/v, 2-day fermentation) achieved 82.2 % protein recovery rate, yielding 15.3 g free amino acids (FAAs) and 17.8 g soluble peptides (SPPs) (96 % < 2000 Da) per 100 g RSM (40.28 g protein). Fermented RSM (FRSM) showed significant improvements: 86.5 % protein digestibility (a 2.6-fold enhancement), and a more enriched nutritional profile with 47.4 % essential amino acids in FAAs. Additionally, SSF effectively reduced anti-nutritional factors, removing 92 % glucosinolates and nearly 100 % phytic acid. Structural analysis revealed that FRSM’s fragmented, porous morphology facilitated improved nutrient absorption. A largemouth bass feeding trial demonstrated the practical benefits of FRSM, where a 10 % replacement in feed increased weight gain rate and feed intake compared to RSM-fed controls. This study provides a scalable and sustainable solution for the valorization of RSM, addressing both protein security and circular economy objectives.

为了满足由人口增长驱动的全球对蛋白质日益增长的需求，将农业副产品转化为可食用蛋白质对可持续粮食安全至关重要。本研究介绍了一种利用蛋白酶工程Streptomyces sp. SCUT-3的固态发酵（SSF）平台，用于世界第二大油籽渣菜籽粕（RSM）的发酵。优化后的SSF参数（底液比1:2 w/v，发酵2天）的蛋白质回收率为82.2 %，每100 g RSM（40.28 g蛋白质）产生15.3 g游离氨基酸（FAAs）和17.8 g可溶性肽（SPPs）（96 % < 2000 Da）。发酵的RSM （FRSM）表现出显著的改善：蛋白质消化率为86.5 %（提高2.6倍），营养成分更丰富，FAAs中必需氨基酸含量为47.4 %。此外，SSF有效地降低了抗营养因子，去除92% %的硫代葡萄糖苷和近100% %的植酸。结构分析表明，FRSM的破碎多孔形态有助于提高营养物质的吸收。一项大口黑鲈饲养试验证明了FRSM的实际效益，在饲料中添加10% %的替代饲料，与rsm饲养的对照组相比，可提高增重率和采食量。该研究为RSM的增值提供了一个可扩展和可持续的解决方案，同时解决了蛋白质安全和循环经济目标。

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引用次数: 0

Membrane transporter mining and enzyme engineering drive high-titer δ-tocotrienol production in Yarrowia lipolytica 膜转运体的挖掘和酶工程驱动高滴度的产脂耶氏菌δ-生育三烯醇

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-04-01 Epub Date: 2026-01-09 DOI: 10.1016/j.bej.2026.110079

Chenchen Xu , Xinyu Jiao , Quanlu Zhao , Tianli Yuan , Haoran Bi , Kai Wang , Tianwei Tan

δ-Tocotrienol, a vitamin E bioactive isoform, possesses strong antioxidant and anticancer properties. However, its production is limited by its extremely low abundance in plants and the difficulty of separating it from other tocotrienol isomers. Therefore, in this study, Y. lipolytica was engineered for high-level δ-tocotrienol production using a multipronged strategy. Heterologous δ-tocotrienol pathway gene expression and precursor supply enhancement facilitated the production of 2.8 mg/L δ-tocotrienol. Transcriptomic analysis and metabolic modeling (iYALI743) identified four transporters that markedly improved the δ-tocotrienol titer to 4.68 mg/L. Moreover, PpHPD and SyHPT protein fusion, and the truncation of the N-terminal signal peptide of AtTC, which enhanced plant enzyme performance in Y. lipolytica, raised the δ-tocotrienol titer to 54.67 mg/L. Under optimized fermentation conditions with antioxidant butylated hydroxytoluene (BHT) supplementation, the engineered strain achieved a δ-tocotrienol titer of 617.23 mg/L in a 2 L bioreactor, representing the highest yield on record.

δ-生育三烯醇是维生素E的一种生物活性异构体，具有很强的抗氧化和抗癌特性。然而，由于其在植物中的丰度极低，并且难以从其他生育三烯醇异构体中分离出来，其生产受到限制。因此，在本研究中，利用多管齐下的策略，对聚脂酵母进行了高水平的δ-生育三烯醇生产。异源δ-生育三烯醇途径基因的表达和前体供应的增加促进了2.8 mg/L δ-生育三烯醇的产生。转录组学分析和代谢模型（iYALI743）鉴定出四种转运蛋白，显著提高了δ-生育三烯醇滴度至4.68 mg/L。此外，PpHPD与SyHPT蛋白融合，截断AtTC的n端信号肽，增强了植物酶在脂聚Y. lipolytica中的表现，使δ-生育三烯醇滴度提高到54.67 mg/L。在添加抗氧化剂丁基羟基甲苯（BHT）的优化发酵条件下，工程菌株在2 L的生物反应器中获得了617.23 mg/L的δ-生育三烯醇滴度，这是有记录以来的最高产量。

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引用次数: 0

Optimum biohydrogen production by combination of fermentation and microbial electrolysis cell: Bioreactor design, scale-up, and technology readiness level analysis 结合发酵和微生物电解细胞的最佳生物制氢：生物反应器设计、放大和技术准备水平分析

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-04-01 Epub Date: 2025-12-31 DOI: 10.1016/j.bej.2025.110066

Vikash, Nitai Basak

The integration of fermentation with microbial electrolysis cells (MECs) presents a novel approach to enhance biohydrogen production from organic residues, thereby overcoming the limitations of traditional fermentation systems. This review critically assesses the current state of this combined technology, focusing on bioreactor engineering, scale-up challenges, and its readiness for commercial application. The fermentation stage effectively converts complex biomass into a volatile fatty acid (VFA) rich effluent, which serves as an ideal substrate for the subsequent MEC to achieve near-complete substrate valorisation and significantly higher hydrogen yields. Analysis of bioreactor design reveals a clear trend toward reducing capital costs through the development of low-cost, non-precious metal cathode catalysts, such as nickel alloys and stainless steel, to replace platinum, as well as the adoption of simplified, membraneless reactor architectures. The comprehensive Technology Readiness Level (TRL) assessment places the system at TRL 3–4. However, scaling this technology from the laboratory to industrial application presents considerable challenges. We conclude that bridging the gap to pilot-scale (TRL 6) requires overcoming specific engineering hurdles such as maintaining high current densities in large-scale reactors, mitigating biofouling, and optimizing various biological and physical factors affecting biohydrogen yield in fermentation and MEC systems.

将发酵与微生物电解细胞（MECs）相结合，为提高有机残留物产氢提供了一种新的途径，从而克服了传统发酵系统的局限性。这篇综述批判性地评估了这种组合技术的现状，重点是生物反应器工程、扩大规模的挑战以及其商业应用的准备情况。发酵阶段有效地将复杂的生物质转化为挥发性脂肪酸（VFA）丰富的排出物，作为后续MEC的理想底物，以实现近乎完全的底物增值和显着提高的氢气产量。对生物反应器设计的分析揭示了一个明显的趋势，即通过开发低成本、非贵金属阴极催化剂（如镍合金和不锈钢）来取代铂，以及采用简化的无膜反应器结构来降低资本成本。综合技术准备水平（TRL）评估将该系统置于TRL 3-4。然而，将这项技术从实验室扩展到工业应用存在相当大的挑战。我们得出的结论是，弥合中试规模（TRL 6）的差距需要克服特定的工程障碍，例如在大型反应器中保持高电流密度，减轻生物污染，以及优化影响发酵和MEC系统中生物氢产量的各种生物和物理因素。

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引用次数: 0

Corrigendum to “Use of free nitrous acid from partial nitrification reactor for the sanitization of digester effluents and Class A biosolids production” [Biochem. Eng. J. 226 (2026) 109993] 部分硝化反应器游离亚硝酸盐用于消化废水的消毒和A类生物固体生产的更正[生物化学]。Eng。J. 226 (2026) 109993]

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-04-01 Epub Date: 2026-01-12 DOI: 10.1016/j.bej.2026.110077

Zeinab Morhell , Gabriel Martínez , Oscar Franchi , Dafne Crutchik , Cristofer Da Silva , Lorna Guerrero , Alba Pedrouso , Ángeles Val del Río , Anuska Mosquera-Corral , José Luis Campos

引用次数: 0

Purple-red azaphilone alkaloids from Talaromyces amestolkiae showing antimicrobial activity 具有抗菌活性的紫红色氮蚜酮生物碱

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-04-01 Epub Date: 2025-12-30 DOI: 10.1016/j.bej.2025.110065

Fernanda de Oliveira , Caio de Azevedo Lima , Diana Cláudia Gouveia Alves Pinto , Laura Carmona Ferreira , Tais Maria Bauab , Silvio Silvério da Silva , Valéria Carvalho Santos-Ebinuma

The global rise of antimicrobial resistance highlights the urgent need for alternative bioactive compounds from sustainable sources. Fungal colorants, especially azaphilones, represent promising natural molecules due to their structural diversity, colorant properties, and biological activities. Talaromyces amestolkiae is a well-known source of Monascus-like azaphilone colorants free of mycotoxins, yet its antimicrobial potential and production dynamics remain underexplored. In this study, submerged cultivations were conducted to improve colorant production, isolate novel compounds, and assess antimicrobial activity. The effects of glucose concentration and hydrodynamic conditions, expressed through agitation, aeration, and the volumetric oxygen transfer coefficient (k_La), were investigated. The optimal condition (100 rpm, 1.0 vvm, 10 g·L⁻¹ of glucose) promoted balanced oxygen availability and carbon utilization, resulting in maximum colorant yield. Two novel purple-red azaphilone alkaloids, cis-amestolkin (1) and trans-amestolkin (2), were isolated and characterized by UV–Vis, LC-HRMS, and NMR spectroscopy. These compounds are geometric isomers of nitrogen-containing azaphilones featuring a γ-lactone ring and a unique 4-amino-6-hydroxyhexanoic acid moiety. Antimicrobial assays revealed that T. amestolkiae-derived metabolites exhibit pronounced inhibitory activity against Pseudomonas aeruginosa, Staphylococcus aureus, and Salmonella setubal, highlighting their bioactive potential . This work integrates bioprocess improvement with metabolite identification and biological evaluation, underscoring the value of fungal colorants as multifunctional, sustainable agents for industrial and pharmaceutical applications.

全球抗菌素耐药性的上升凸显了从可持续来源寻找替代生物活性化合物的迫切需要。真菌着色剂，特别是氮唑啉，由于其结构多样性、着色剂特性和生物活性，是一种很有前途的天然分子。amestolkiae Talaromyces amestolkiae是一种众所周知的不含霉菌毒素的红曲霉样氮蚜酮着色剂的来源，但其抗菌潜力和生产动态仍未得到充分开发。在本研究中，通过潜水培养来提高着色剂的产量，分离新的化合物，并评估抗菌活性。考察了葡萄糖浓度和流体动力条件对搅拌、曝气和体积氧传递系数（kLa）的影响。最佳条件（100 rpm, 1.0 vvm， 10 g·L⁻¹葡萄糖）促进了氧可用性和碳利用的平衡，从而获得了最大的着色剂收率。分离得到了两种新的紫红色氮蚜酮生物碱顺式-阿梅斯托金(1)和反式阿梅斯托金(2)，并通过UV-Vis、LC-HRMS和NMR对其进行了表征。这些化合物是含氮氮氮酮的几何异构体，具有γ-内酯环和独特的4-氨基-6-羟基己酸部分。抗菌实验显示，金黄色葡萄球菌衍生的代谢物对铜绿假单胞菌、金黄色葡萄球菌和settubal沙门氏菌具有明显的抑制活性，突出了其生物活性潜力。这项工作将生物过程改进与代谢物鉴定和生物学评价相结合，强调了真菌着色剂作为多功能、可持续的工业和制药应用剂的价值。

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引用次数: 0

Lignin valorization to polyhydroxyalkanoates via microbial co-cultured and genome reduction method 微生物共培养和基因组还原法研究木质素转化为聚羟基烷酸酯

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-04-01 Epub Date: 2026-01-12 DOI: 10.1016/j.bej.2026.110080

Yi-Fei Zhang , Dong Wang , Yin-Ping Wang , Zhong-Wei Fan , Jian Liu , Shi-Lei Yu , Jia-Qi Cui

The utilization of agricultural waste as a renewable carbon source for biopolymer production represents a critical component of the circular economy. To enhance the bioconversion efficiency of bio-based polymers, a co-cultured system and a genome reduction method were employed to improve lignin valorization. Pseudomonas putida KT2440 exhibited higher lignin bioconversion capacity than other evaluated strains. When co-cultured with Bacillus sp. S0, the dry cell weight (DCW) and polyhydroxyalkanoate (PHA) concentration increased to 1.6 g/L and 42.6 mg/L, respectively, compared with P. putida KT2440 alone. Lignin valorization was further enhanced when the genome-reduced strain P. putida KTU-13 was co-cultured with Bacillus sp. S0. After optimizing key fermentation parameters, including nitrogen content, inoculation rate, and strain ratio, the DCW and PHA concentration reached 2.0 g/L and 67.1 mg/L, respectively. Overall, this work demonstrates a sustainable approach for improving bio-based polymer production through integrated process optimization.

利用农业废弃物作为生物聚合物生产的可再生碳源是循环经济的重要组成部分。为了提高生物基聚合物的生物转化效率，采用共培养体系和基因组还原方法来改善木质素的增值。恶臭假单胞菌KT2440表现出较高的木质素转化能力。与枯草芽孢杆菌KT2440共培养时，枯草芽孢杆菌的干细胞重（DCW）和聚羟基烷酸酯（PHA）浓度分别提高到1.6 g/L和42.6 mg/L。当基因组减少的恶臭杆菌KTU-13与芽孢杆菌S0共培养时，木质素的增殖能力进一步增强。对氮含量、接种量、菌种比等关键发酵参数进行优化后，DCW和PHA浓度分别达到2.0 g/L和67.1 mg/L。总的来说，这项工作证明了通过集成工艺优化改善生物基聚合物生产的可持续方法。

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引用次数: 0

Sustainable chitosan-egg white protein nanofiber membranes for efficient bacterial capture and killing 可持续的壳聚糖-蛋清蛋白纳米纤维膜，用于有效的细菌捕获和杀死

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-04-01 Epub Date: 2026-01-02 DOI: 10.1016/j.bej.2025.110067

Thi Tam An Tran , Edouard Gnoumou , Quang-Vinh Le , Nguyen The Duc Hanh , Nanthiya Hansupalak , Yi-Hua Gao , Bing-Lan Liu , Chi-Yun Wang , Kuei-Hsiang Chen , Yu-Kaung Chang

Egg whites are an abundant source of natural proteins with inherent biological activities, including antibacterial properties. In this study, a novel antibacterial nanofiber membrane was developed by immobilizing egg white proteins (EWP) onto an electrospun polyacrylonitrile (PAN) membrane. The PAN membrane was first modified via alkaline hydrolysis to introduce carboxyl groups (P-COOH), followed by chitosan (CS) grafting (P-COOH-CS) to enhance biocompatibility and protein attachment. EWP was then immobilized onto the surface through ionic and covalent interactions. The immobilization process was optimized by varying pH, CS molecular weight, and solute concentration. The resulting P-COOH-CS-EWP membrane exhibited strong antibacterial efficacy, achieving 98.25 ± 2.09 % reduction in Gram-negative E. coli and 88.24 ± 2.93 % reduction in Gram-positive S. aureus. Furthermore, the nanofiber membrane demonstrated good reusability and stability, retaining 75.84 ± 3.73 % activity after three cycles and 76.41 ± 0.79 % after four weeks of storage. This work highlights the potential of EWP-functionalized nanofiber membranes as a cost-effective, eco-friendly antibacterial material. The approach provides a sustainable strategy for recycling EWP waste and can be applied in biomedical, food packaging, and environmental applications that require antibacterial protection.

蛋清是天然蛋白质的丰富来源，具有固有的生物活性，包括抗菌特性。本研究将蛋清蛋白（EWP）固定在静电纺聚丙烯腈（PAN）膜上，制备了一种新型抗菌纳米纤维膜。首先对PAN膜进行碱性水解修饰，引入羧基（P-COOH），然后进行壳聚糖（CS）接枝（P-COOH-CS）以增强生物相容性和蛋白质附着。然后通过离子和共价相互作用将EWP固定在表面。通过改变pH、CS分子量和溶质浓度对固定化工艺进行优化。所得P-COOH-CS-EWP膜具有较强的抗菌效果，对革兰氏阴性大肠杆菌和革兰氏阳性金黄色葡萄球菌的抗菌效果分别达到98.25 ± 2.09 %和88.24 ± 2.93 %。此外，纳米纤维膜表现出良好的可重复使用性和稳定性，三次循环后活性保持75.84 ± 3.73 %，四周后活性保持76.41 ± 0.79 %。这项工作强调了ewp功能化纳米纤维膜作为一种具有成本效益，环保的抗菌材料的潜力。该方法为EWP废物的回收提供了一种可持续的策略，可以应用于需要抗菌保护的生物医学，食品包装和环境应用。

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