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Evaluation of Melanoidin Contribution to Colour Characteristics of Brewing Products 类黑素对酿酒产品颜色特征贡献的评价
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20235703001
Irina A. Gribkova, M. Eliseev, O. Kosareva, M. Zakharov, V. Zakharova
The article focuses on the issue of melanoidin quantification and its influence on the color characteristics of brewing products. It is shown that the reaction conditions (100±2)°C, the processing time of 2 hours and a certain list of sugars and amino acids typical for brewing products are adequate to obtain linear dependencies for estimating the beer melanoidin content at 300 and 420 nm. The data on the total content of melanoidins in light beer in the range of 3.83-51.4 mg/dm3, and in dark beer – 6.82-145.94 mg/dm3 at the specified wavelengths are given. The authors found that the dependence between the color and the total beer melanoidin content is characterized by a correlation coefficient R = 0.624, which indicates the influence of other organic compounds of beer on the color intensity.
本文主要讨论了类黑素的定量问题及其对酿酒产品颜色特性的影响。结果表明,在100±2°C的反应条件下,2小时的处理时间和一定的酿酒产品典型糖和氨基酸列表足以获得线性依赖关系,用于估算300和420 nm的啤酒类黑素含量。给出了在特定波长下,淡啤酒中类黑素总含量为3.83 ~ 51.4 mg/dm3,黑啤酒中类黑素总含量为6.82 ~ 145.94 mg/dm3。结果表明,啤酒颜色与啤酒总类黑素含量之间存在相关性,相关系数R = 0.624,说明啤酒中其他有机物对颜色强度的影响。
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引用次数: 2
Preparation, Assay and Stability Analysis of Curcumin-Polysaccharide Complexes 姜黄素-多糖配合物的制备、测定及稳定性分析
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236001012
Yun Qiao
Curcumin is a hydrophobic plant polyphenol with various functional properties such as antioxidant, antibacterial, anti-inflammatory, blood lipid regulator, hypotensive and anti-tumour, etc. However, it is sensitive to environmental factors such as light, oxygen and heat, and is prone to oxidative degradation and loss of biological activity during processing, storage and transportation, thus limiting its application. In this paper, chitosan and curcumin were used to form a complex to encapsulate and protect curcumin, and some modern assay techniques were used to examine the structural and functional properties of the complex and analyze its stability. RESULTS: Chitosan and curcumin could form a nanocomplex and the resistance of curcumin to light and heat was greatly enhanced after the formation of the complex. Significance: This study is of great importance to solve the problems of poor water dispersion, unstable physicochemical properties and difficult application of curcumin, and is of academic and practical significance for the future development and utilization of curcumin.
姜黄素是一种疏水性植物多酚,具有抗氧化、抗菌、抗炎、调节血脂、降血压、抗肿瘤等多种功能特性。但它对光、氧、热等环境因素敏感,在加工、储存和运输过程中容易发生氧化降解,丧失生物活性,限制了其应用。本文以壳聚糖和姜黄素为材料,制备了包封和保护姜黄素的配合物,并利用现代分析技术对配合物的结构和功能特性进行了表征,分析了其稳定性。结果:壳聚糖与姜黄素可形成纳米复合物,复合物形成后姜黄素的抗光性和抗热性明显增强。意义:本研究对解决姜黄素水分散性差、理化性质不稳定、应用困难等问题具有重要意义,对姜黄素未来的开发利用具有重要的学术意义和现实意义。
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引用次数: 0
The role of mechanical channel in the proliferation and migration of colon cancer 机械通道在结肠癌增殖和迁移中的作用
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236101024
Pin-Yu Wang
Piezo channel is the first family of mechanically gated cation channels found in mammals and has been shown correlated with the proliferation and metastasis of tumors in recent years. In a variety of cancer tissues, colon cancer tissues are particularly affected by mechanical stimulation. In this paper, we reported that the role of piezo1 in the proliferation and migration of colon cancer. Firstly, bioinformatics indicated that high levels of piezo1 expression existed in CRC tissues and were associated with poor prognosis. In vitro experiments, SW1116 cells were cultured in DMSO solutions, and CCK-8 assay and transwell assay were separately performed. It turned out that the inhibition of PIEZO1 by Dooku1 resulted in diminished cell proliferation and metastasis, while the activation of PIEZO1 by Yoda1 led to enhanced proliferation and migration of colon cells. Collectively, these findings demonstrated the facilitation role of piezo1 in colon cancer proliferation and migration, suggesting that piezo1 has the potential as a novel therapeutic target for drug design and treatment of colon cancer.
压电离子通道是在哺乳动物中发现的第一个机械门控阳离子通道家族,近年来被证明与肿瘤的增殖和转移有关。在多种癌症组织中,结肠癌组织尤其受机械刺激的影响。在本文中,我们报道了piezo1在结肠癌增殖和迁移中的作用。首先,生物信息学表明,在结直肠癌组织中存在高水平的piezo1表达,并与不良预后相关。体外实验采用DMSO溶液培养SW1116细胞,分别进行CCK-8测定和transwell测定。结果表明,Dooku1对PIEZO1的抑制导致细胞增殖和转移减少,而Yoda1对PIEZO1的激活导致结肠细胞增殖和迁移增强。总之,这些发现证明了piezo1在结肠癌增殖和迁移中的促进作用,表明piezo1具有作为结肠癌药物设计和治疗的新治疗靶点的潜力。
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引用次数: 0
The resistance of a number of indigenous species of shrubs and subshrubs of Sakhalin to a low-snow winter 库页岛一些本土灌木和亚灌木物种对低雪冬季的抵抗力
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236601008
V. V. Sheiko, D. M. Lebedev, V. Sorokopudov
A negative reaction to the anomalous nature of the low-snow winter of 2018/19 was shown by representatives of 78% of the species of shrubs and subshrubs indigenous to Sakhalin island, studied in the botanical garden of the city of Yuzhno-Sakhalinsk, and 9% of species of foreign shrubs. Such a reaction of local plants can be associated with their adaptations to such factors as a thick snow cover, temperature stability in spruce-fir forests, weak soil freezing. The latter may be due to deep snow, high groundwater levels, or if the species is adapted to the warmer climate of the main part of the range.
在南萨哈林斯克市植物园进行的研究中,78%的库页岛本土灌木和亚灌木物种以及9%的外来灌木物种的代表对2018/19年低雪冬季的异常性质表现出负面反应。当地植物的这种反应可能与它们对诸如厚厚的积雪、云杉林的温度稳定性、弱土壤冻结等因素的适应有关。后者可能是由于深雪、高地下水位,或者如果该物种适应了范围主要部分的温暖气候。
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引用次数: 0
Opportunities for monitoring the comfort of children living in substitute families for sustainable social development 有机会监测生活在替代家庭中的儿童的舒适度,以促进可持续的社会发展
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236506001
Maria Zaluzhnaya, Elena Bayer, Elena Chekalenko, N. Grigoryan, Alla Illarionova
This article reflects the monitoring indicators from the research of living conditions in substitute families in the South of Russia. The authors present some study results of the living comfort in a substitute family, namely the relationships with foster parents; interaction with blood and other foster children in the family; the psycho-emotional state; physical, mental, and emotional violence in a foster family; the desire to live in a substitute family. The monitoring was carried out among 76 foster children being brought up in substitute families of the Azov city and the Azov district of the Rostov region and accompanied by the Azov Child Care Centre. The results obtained are of great importance for specialists of support services for substitute families, as well as researchers whose scientific interest is focused on studying substitute family care in Russia. The research has been carried out within the framework of the Ministry of General and Vocational Education order of the Rostov Region No. 747 of 28 July 2022 “On measures to assess the level of comfort of a child’s stay in a substitute family”.
本文通过对俄罗斯南部替代家庭生活状况的研究,反映了监测指标。作者给出了一些关于替代家庭生活舒适度的研究结果,即与养父母的关系;与家庭中的血亲和其他寄养儿童的互动;心理-情绪状态;寄养家庭中的身体、精神和情感暴力;想要住在替代家庭的愿望。对76名寄养儿童进行了监测,这些儿童在罗斯托夫州亚速市和亚速区的替代家庭中长大,并由亚速儿童保育中心陪同。所获得的结果对替代家庭支持服务的专家以及研究俄罗斯替代家庭护理的科学兴趣的研究人员具有重要意义。这项研究是在罗斯托夫州普通和职业教育部2022年7月28日第747号命令“关于评估儿童在替代家庭居住的舒适程度的措施”的框架内进行的。
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引用次数: 0
Current Environmental Problems and their Solutions with the Help of New Technologies 当前的环境问题及其新技术的解决方案
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236307012
Rayana Duchaeva, S. Magomadov
The problem of ecology in these times is becoming more acute every day. An environmental problem is a change in the natural environment that leads to disruption of the functioning and structure of nature. Global problems are generated by the contradictions of social development, affecting the surrounding world by a sharply increased scale of human activity. Also associated with the uneven scientific, technical and socio-economic development of countries and regions. From the point of view of modern scientists, humanity lives in a world where everything is already collapsing.
当今时代的生态问题日益尖锐。环境问题是指自然环境的变化导致自然功能和结构的破坏。全球性问题是由社会发展的矛盾产生的,人类活动规模的急剧增加影响着周围的世界。也与各国和各区域科学、技术和社会经济发展不平衡有关。从现代科学家的观点来看,人类生活在一个一切都在崩溃的世界里。
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引用次数: 0
Same-day correction of baselines for demand response using long short-term memory 利用长短期记忆对需求响应基线进行同日修正
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236204004
T. Nagaya, Yoshifumi Hatai, K. Sano, Yuho Hane, Yasuto Matsui
In incentive-based the Demand Response, the amount of electricity demand reduction is calculated by subtracting actual electricity demand from the baseline (BL). The BL is the estimated electricity demand of households when no electricity demand suppression is performed. In Japan, the high 4 of 5 method is used to forecast the BL by averaging the actual demand of the day. In this study, we refer to the high 4 of 5 method as BL1. BL2 is the BL to which the value of the same-day adjustment is added based on the actual demand of the day. BL3 is BL1 plus the value of the same-day adjustment predicted using Long Short-Term Memory (LSTM). The average MAE values for BL2 and BL3, calculated using actual electricity demand data from October 15, 2021, to December 24, 2021, were 11.2 kW and 8.1 kW, respectively, with BL3 being 3.1 kW smaller than BL2. To estimate the confidence intervals for BL2 and BL3, we calculated the error by subtracting each BL from the actual value and calculated the ±3σ equivalent for the distribution of the error. The confidence interval calculated for BL3 was found to be ±9.2 kW lower than that for BL2. The F-test for the distribution of the errors for BL2 and BL3 yielded a P-value of 4.05 × 10-50, indicating that the variances of the two distributions were not equally distributed.
在以激励为基础的电力需求回应方案中,减少的电力需求量是以基线减去实际电力需求来计算的。BL是在不抑制电力需求的情况下,住户的估计电力需求。在日本,高4 / 5的方法是通过平均当天的实际需求来预测BL。在本研究中,我们将5的高4法称为BL1。BL2是根据当天实际需求加上当日调整值的提单。BL3为BL1加上使用长短期记忆(LSTM)预测的当日调整值。根据2021年10月15日至12月24日的实际电力需求数据计算,BL2和BL3的平均MAE值分别为11.2 kW和8.1 kW,其中BL3比BL2小3.1 kW。为了估计BL2和BL3的置信区间,我们通过从实际值中减去每个BL来计算误差,并计算误差分布的±3σ当量。BL3计算的置信区间比BL2低±9.2 kW。对BL2和BL3的误差分布进行f检验,p值为4.05 × 10-50,说明两种分布的方差分布不均匀。
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引用次数: 0
Development of Agriculture in the Context of Geopolitical Tensions 地缘政治紧张背景下的农业发展
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236301003
R. Yalmaev, E. Shkarupa, N. Gorshkova
The conditions of instability in the country, due to economic and political aspects, dictate the need to expand and strengthen the agricultural potential that can provide the country’s population with food. Despite geopolitical tensions, domestic agriculture has shown decent results in the past year, with some production volumes reaching scale. The purpose of this study is to study the key trends in the development of agriculture, the emphasis is placed primarily on crop production. Realizing the importance of ensuring food security, the tasks of the state should be to create stimulating conditions for intensifying the process of import substitution. It is necessary to expand the potential of domestic breeding, the development prospects of which are seen in the application of the “triple helix” concept (state-business-science). Providing farmers with highly effective plant protection products is also an important component of the agrarian policy of the state, which ensures, first of all, an increase in production and, as a result, consumption. In 2023, the mechanism of state support is being improved; appropriately raised the issue of adjusting the directions and priorities due to sanctions pressure and a shift in emphasis towards the policy of import substitution.
由于经济和政治方面的原因,该国的不稳定状况决定了必须扩大和加强能够向该国人口提供粮食的农业潜力。尽管地缘政治局势紧张,但国内农业在过去一年取得了不错的成果,一些产量达到了规模。本研究的目的是研究农业发展的主要趋势,重点主要放在作物生产上。认识到保障粮食安全的重要性,国家的任务应该是为加强进口替代进程创造有利条件。扩大国内育种的潜力是必要的,其发展前景是应用“三螺旋”概念(国家-商业-科学)。向农民提供高效的植物保护产品也是国家土地政策的重要组成部分,这首先保证了产量的增加,从而保证了消费的增加。2023年,国家支持机制不断完善;适当地提出了由于制裁压力和重点转向进口替代政策而调整方向和优先次序的问题。
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引用次数: 0
Evaluation of bio-acidifying yeast Lachancea thermotolerans as a strategy to reduce the effects of climate change in Tempranillo grape must vinification 耐高温生物酸化酵母菌Lachancea在丹尼罗葡萄酿造过程中减少气候变化影响的研究
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20235602036
Vidal Antonio Pérez Muñoz, Liliana del Rocío Castro-López, Luis Eugenio Martínez Hernández, Davis Cordero Herrera, Gabriela Herrera Martínez, Guillermo Castillo
The effects of climate change are posing major challenges for winemaking, especially in warm regions. Rising temperatures are leading to a considerable increase in sugar content and a reduction in the acidity of grape juices. This has prompted the search for new chemical and biological tools to reduce these effects in winemaking. Lachancea thermotolerans represent an interesting tool for wine improvement, due to their effects on lactic acid, ethanol, and volatile acidity. In this study, we evaluated the sequential inoculation of Lachancea thermotolerans (AEB Italia) + Saccharomyces cerevisiae as an alternative to the addition of water and tartaric acid in the vinification of over-ripened wine must (Tempranillo cv.). To do so, we conducted a micro vinification experiment that included three experimental treatments (replicated four times); AAS = Addition of water + tartaric acid fermented with Saccharomyces cerevisiae, ALS = sequential fermentation with Lachancea thermotolerans followed by Saccharomyces cerevisiae and SAC = Only inoculation with Saccharomyces cerevisiae (control). We measured the lactic acid, malic acid, acetic acid, total acidity, pH, glucose + fructose, and ethanol concentration of the resulting wines. We found a higher lactic acid content and total acidity in ALS wines (2.2 g/L y 6.65 g/L) compared with AAS (0.13 g/L y 5.75 g/L) and SAC wines (0.18 g/L y 5.37 g/L). ALS wines showed a significantly lower pH (4.00) than SAC wines (4.19) but did not differ significantly from AAS wines (3.19). Finally, acetic acid was higher in ALS wines (0.89 g/L) than AAS wines (0.64 g/L) but did not differ significantly from SAC wines (1.02 g/L). Our results point out that the use of Lachancea thermotolerans in a sequential inoculation with Saccharomyces cerevisiae is an alternative to the addition of water and tartaric acid in the vinification of over-ripened Tempranillo musts.
气候变化的影响给葡萄酒酿造带来了重大挑战,尤其是在温暖地区。气温上升导致葡萄汁的含糖量大幅增加,酸度降低。这促使人们寻找新的化学和生物工具来减少葡萄酒酿造中的这些影响。由于其对乳酸、乙醇和挥发性酸度的影响,耐温菌代表了葡萄酒改良的一个有趣的工具。在这项研究中,我们评估了顺序接种耐高温Lachancea (AEB Italia) +酿酒酵母(Saccharomyces cerevisiae)作为在过熟葡萄酒(Tempranillo cv.)酿造过程中添加水和酒石酸的替代方法。为此,我们进行了一个微发酵实验,包括三个实验处理(重复四次);AAS =加入水+酒石酸发酵的酿酒酵母,ALS =先用耐温酿酒酵母连续发酵,然后再用酿酒酵母发酵,SAC =只接种酿酒酵母(对照)。我们测量了所得葡萄酒的乳酸、苹果酸、乙酸、总酸度、pH、葡萄糖+果糖和乙醇浓度。我们发现ALS葡萄酒的乳酸含量和总酸度(2.2 g/L和6.65 g/L)高于AAS葡萄酒(0.13 g/L和5.75 g/L)和SAC葡萄酒(0.18 g/L和5.37 g/L)。ALS葡萄酒的pH值(4.00)明显低于SAC葡萄酒(4.19),但与AAS葡萄酒(3.19)差异不显著。最后,ALS葡萄酒的乙酸含量(0.89 g/L)高于AAS葡萄酒(0.64 g/L),但与SAC葡萄酒(1.02 g/L)差异不显著。我们的研究结果表明,在连续接种酿酒酵母菌中使用耐高温菌可以替代在过熟的丹魄葡萄酒酿造过程中添加水和酒石酸。
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引用次数: 0
Determination the content of cysteine in food by potassium dichromate spectrophotometry 重铬酸钾分光光度法测定食品中半胱氨酸含量
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20235901012
Xin-rong Wen, C. Tu
In this paper, a new method for the determination of cysteine by potassium dichromate spectrophotometry is established. In the H2SO4 medium, potassium dichromate has strong oxidation. Potassium dichromate can react with the hydrosulfuryl(-SH) in cysteine, which makes potassium dichromate fade, and the fading degree of potassium dichromate is proportional to the amount of cysteine added. Therefore, the content of cysteine can be quantitatively determined by determinating the decrease value of absorbance. The various influencing factors for the determination the content of cysteine by potassium bichromate spectrophotometry are investigated. Under the optimal conditions, the content of cysteine in food is determined, and the results are satisfactory.
本文建立了重铬酸钾分光光度法测定半胱氨酸的新方法。重铬酸钾在H2SO4介质中具有很强的氧化性。重铬酸钾能与半胱氨酸中的氢硫酰(-SH)发生反应,使重铬酸钾褪色,重铬酸钾的褪色程度与半胱氨酸的加入量成正比。因此,可以通过测定吸光度降低值来定量测定半胱氨酸的含量。研究了重铬酸钾分光光度法测定半胱氨酸含量的各种影响因素。在此条件下,对食品中半胱氨酸的含量进行了测定,结果令人满意。
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引用次数: 0
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