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The T-RFLP research method in the study of rumen microbiota in dairy cows with subclinical ketosis T-RFLP研究方法在亚临床酮症奶牛瘤胃微生物群研究中的应用
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236606001
A. Yashin, Elizabeth Kasatkina, Aleksey Prusakov, Vladimir Ponamarev, Alexander Lunegov
The article presents the results of researches related to the study of the possibility of using the modern molecular genetics method -T-RFLP-analysis (terminal restriction fragment length polymorphism) to identify the community of microorganisms in the contents of the rumen in clinically healthy highly productive cows and cows with subclinical ketosis. The research methodology is based on the analysis of the conservative regions in the microorganisms’ genome variability. The results showed the high efficiency of the method used for the identification of microorganisms in the rumen contents in the studied animals. A large community of bacteria, archaea, protozoa and anaerobic fungi is determined in the rumen contents from highly productive cows with subclinical ketosis. The data obtained allow us to significantly amplify the information on the pathogenesis of subclinical ketosis in cows with high milk productivity. The presence of conditionally pathogenic and pathogenic microflora in sick cows in the rumen contents indicates a violation of rumen digestion, which leads to the development of concomitant non-contagious diseases in animals.
本文介绍了利用现代分子遗传学方法- t - rflp分析(末端限制性片段长度多态性)鉴定临床健康高产奶牛和亚临床酮症奶牛瘤胃内容物微生物群落的可能性的相关研究结果。研究方法是基于对微生物基因组变异中的保守区域的分析。结果表明,该方法对实验动物瘤胃内容物微生物的鉴定具有较高的效率。在高产亚临床酮症奶牛的瘤胃内容物中发现了大量细菌、古生菌、原生动物和厌氧真菌。获得的数据使我们能够显著地扩大高产奶量奶牛亚临床酮症发病机制的信息。在病牛的瘤胃内容物中存在条件致病性和致病性微生物群,表明违反了瘤胃消化,从而导致动物同时发生非传染性疾病。
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引用次数: 0
Social and psychological state as a factor for determining pronosological health 社会和心理状态是决定生理健康的一个因素
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236505028
A. A. Abdumananov, R. M. Eshonov
The article shows the development of a continuous functioning technology for remote automated monitoring of the socio-psychological state of the population to determine the prenosological level of individual health, which allows to optimize medical non-invasive mass screening of the population. The technologies and their implementation are shown to ensure functioning with the use of information and communication technologies, mobile communications, methods of automated remote exchange, accumulation, storage of information, as well as with the involvement of medical knowledge, mathematical models and algorithms for their data mining socio-psychological state population, development and implementation of preventive measures, which are effective mechanisms to ensure health of the healthy people’s.
本文展示了一种持续功能技术的发展,用于远程自动监测人口的社会心理状态,以确定个人健康的先兆学水平,从而可以优化人口的医疗非侵入性大规模筛查。这些技术及其实施表明,通过使用信息和通信技术、移动通信、自动远程交换、信息积累和存储方法,以及利用医学知识、数学模型和算法进行数据挖掘、社会心理状况、人口、制定和实施预防措施,确保发挥作用。这些都是保障健康人健康的有效机制。
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引用次数: 0
Improving audition costs of agricultural products under economic modernization 经济现代化条件下提高农产品成本
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236508001
Dilshod Mamadiyarov, Shakhlokhon Dekhkanova, Dilobar Khaitboeva, Feruza Uzokova
General production costs are reflected in the item “Costs of production and organization of management”. This article also takes into account insurance payments for buildings, structures, machinery, equipment, inventory and other fixed assets directly used in the production of products. Insurance payments are distributed by crops and types of products in proportion to the costs of production. Insurance payments are reflected in the accounting records in the debit of the “Agriculture” account and in the credit of the “Insurance settlements” account. The article “Other expenses” reflects the expenses directly related to the production of products, but none of the above items is included. This article reveals modern methods of intra-company management of the formation of the cost of agricultural products, the composition of the costs of agricultural firms, the procedure for their control, optimization and audit. A structural model is proposed that represents the methodology of internal control and audit at the macro- and micro-levels of the hierarchical management of an agricultural enterprise. The composition of the information necessary for the implementation of the methodology of intra-company control, accounting and analytical procedures used to evaluate cost items and make management decisions are determined. The features of the audit (external, internal) of production costs are revealed - establishing the validity of the formation and correctness of accounting for production costs, identifying unused reserves. The conceptual scheme of the technique of this type of audit is given.
一般生产成本反映在“生产和组织管理成本”项目中。本文还考虑了建筑物、构筑物、机器、设备、存货和其他直接用于产品生产的固定资产的保险支付。保险费按作物和产品种类按生产成本的比例分配。保险付款在会计记录中反映在“农业”帐户的借方和“保险结算”帐户的贷方。“其他费用”一栏反映的是与产品生产直接相关的费用,但不包括上述各项。本文揭示了农产品成本形成的现代企业内部管理方法,农业企业成本的构成,农业企业成本的控制、优化和审计程序。本文提出了一个结构模型,代表了农业企业分级管理的宏观和微观层面的内部控制和审计方法。确定了实施公司内部控制、会计和分析程序的方法所需的信息的组成,这些方法用于评估成本项目和做出管理决策。揭示了生产成本审计(外部审计和内部审计)的特点——建立生产成本核算形成的有效性和正确性,识别未使用准备金。给出了这种审计技术的概念方案。
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引用次数: 0
Forming neuroendocrine apparatus of lung in ontogenesis 个体发生过程中肺神经内分泌器官的形成
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236505015
S. Blinova, F. Oripov, N. Yuldasheva, F. M. Khamidova, J. M. Ismoilov
Aim of the research is to show the developing legitimacy APUD-system of lungs in pre and post ontogenesis. Condition of neuroendocrine apparatus of lungs on rabbits was studied during the fetal embryogenesis of inner development and after 1-180 days of the birth. Duration of the research on rabbits was chosen according to their changing afterbirth features as physiological function of organism; was selected the periods of newborn stage, enlightenment, complication of locomotion, pre-pubertal and pubertal. Materials were fixed by immersion in liquid of Buena. After suitable wiring, the material was flooded in paraffin. Histologic shears were colored hematoxylin and eosin, stain of Van –Gieson, resorcinol-fuchsinol of Veingeirt. For detecting endocrine cells of shear impregnated by the method of Gremeluise. Luminescent histochemical research was done with the help of V.N. Shvalyova and N.N. Juchkova method with applying glyoxylic acid on fresh-iced shears. For defining, the amount of fluorescent monoamine (serotonin and catecholamine) was used microfluorimetry. However, the intensification of histogenetic processes occurring in the organ after birth due to the expansion of physiological functions is accompanied by an increase in the number of apudocytes and NET. The content of catecholamines and serotonin in neuroendocrine structures depends on the period of growth and differentiation of the lung: during the intensive growth of the respiratory organs in the fetal period and in newborns, the level of catecholamines in apudocytes and NET is increased; with the predominance of differentiation processes in the neuroendocrine apparatus, the serotonin content increases.
本研究旨在揭示apud系统在个体发生前后的发展合法性。研究了兔胚胎发育初期及出生后1 ~ 180天肺神经内分泌器官的发育状况。根据家兔作为机体生理功能的变化特征选择研究时间;选取新生儿期、启蒙期、运动并发症期、青春期前期和青春期。将材料浸泡在Buena液体中固定。在适当的布线后,将材料浸泡在石蜡中。组织切片采用苏木精和伊红染色,Van -Gieson染色,Veingeirt染色。目的:探讨用Gremeluise法浸渍的剪切内分泌细胞的检测方法。采用V.N. Shvalyova法和N.N. Juchkova法,用乙醛酸涂敷新鲜冰剪,进行荧光组织化学研究。为了确定荧光单胺(血清素和儿茶酚胺)的量,使用微荧光法。然而,由于生理功能的扩展,出生后器官中发生的组织发生过程的增强伴随着无轴细胞和NET数量的增加。神经内分泌结构中儿茶酚胺和5 -羟色胺的含量与肺的生长和分化期有关:在胎儿期和新生儿呼吸器官的密集生长期间,apudoc细胞和NET中儿茶酚胺的水平升高;随着神经内分泌器官分化过程的主导,血清素含量增加。
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引用次数: 0
Environmental Problems and Entrepreneurship in the Region 区域内的环境问题和企业家精神
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236304001
V. Dzobelova, Kirill Grabovy, Darya B. Belinskaya
Ensuring environmental protection, rational use and reproduction of natural resources is one of the key constitutionally significant benefits that form the basis of long-term socio-economic development, determine the material basis for the development of future generations. The solution to this problem can be achieved through various tools, in particular through the development of an environmentally oriented business. This article discusses the key features of environmental entrepreneurship at the regional level.
确保环境保护、合理利用和再生产自然资源是构成长期社会经济发展的基础、决定后代发展的物质基础的重要的、具有宪法意义的利益之一。这个问题的解决方案可以通过各种工具来实现,特别是通过发展以环境为导向的业务。本文讨论了区域层面环境企业家精神的主要特征。
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引用次数: 0
Response Surface Optimization of Pure Natural Fairy Tofu Production Process 纯天然神仙豆腐生产工艺响应面优化
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236002023
Chao He, Lebin Yin, Wen-Tao Zou, Cong Liu, Xuewei Yang, Xueyun Luo, Jianping Dai
This experiment was centred on the concept of using pectin gel to manufacture fairy tofu in order to determine the ideal parameters for the creation of natural fairy tofu. Based on the single-factor test, The Box-Behnken central combination design approach was employed to investigate the optimal production process of fairy tofu. According to the findings, there were substantial changes (P<0.05) in the qualitative attributes of tofu at varied material-to-liquid ratios, drying temperatures (the leaves of Premna microphylla Turcz) and grinding temperatures during the production process. The optimal process parameters were: material-to-liquid ratio of 1:60 (g:g), drying temperature of 20 °C and a grinding temperature of 90 °C. The yield of fairy tofu produced under this process parameter was 46.02 g/g, which was 55.33% higher than the yield of fairy tofu produced by the unoptimized process. It indicated that the optimized process conditions for the production of pure natural fairy tofu are feasible and can produce a high-yield and high-quality of fairy tofu. This study provides a theoretical basis for the reuse of the leaves of Premna microphylla Turcz. and lays the foundation for the large-scale production of fairy tofu.
本实验围绕用果胶凝胶制作神仙豆腐的概念,以确定制作天然神仙豆腐的理想参数。在单因素试验的基础上,采用Box-Behnken中心组合设计方法,研究神仙豆腐的最佳生产工艺。结果表明,在不同料液比、干燥温度(小叶豆叶)和研磨温度下,豆腐的定性属性发生了显著变化(P<0.05)。最佳工艺参数为:料液比1:60 (g:g),干燥温度20℃,研磨温度90℃。该工艺参数下的神仙豆腐得率为46.02 g/g,比未优化工艺下神仙豆腐得率提高了55.33%。结果表明,优化后的生产纯天然神仙豆腐的工艺条件是可行的,可以生产出高产、优质的神仙豆腐。本研究为小叶羊草叶片的再利用提供了理论依据。为神仙豆腐的规模化生产奠定了基础。
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引用次数: 0
The Mechanism of Carbon Regulation of Emissions in Industrial Energy 工业能源碳排放调控机制研究
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236302003
Kh. M. Musaeva, Gulpam Annadurdyeva
The introduction of cross-border taxes on hydrocarbon-intensive goods in the EU is expected in 2023, and the draft proposals are expected in the second quarter of 2021. The conditions for collecting such a tax from the EU will affect the interests of the EU itself for the next two decades remaining under the Green Deal until zero emissions. Apparently, the mechanism of tax collection will soon become clear, where and for what the collected funds will be directed. The issue of the impact of new taxes on Russian exports shifts the internal debate over the nature of such regulation to several urgent issues. This is the need for a more accurate, reliable and documented accounting of emissions in the country. And this is the choice of the option of adapting the country’s tax system to minimize the losses of companies, which began to be actively discussed, since the time factor begins to operate. An important aspect of the problem is the need for the simultaneous and rapid introduction of measures in the field of regulation, taxes, statistics in a complex, depending on the chosen response option. The new measures will not only affect the country’s climate programs, taxation and foreign trade, but will also have implications for economic strategy and even regional development.
欧盟预计将于2023年对碳氢化合物密集型商品征收跨境税,提案草案预计将于2021年第二季度出台。从欧盟征收此类税收的条件将影响欧盟自身在绿色协议下的未来20年的利益,直到零排放。显然,税收征收机制将很快变得清晰,征收的资金将用于何处和什么目的。新税对俄罗斯出口产品的影响问题,将围绕此类监管性质的内部辩论转移到了几个紧迫问题上。这就是需要对该国的排放量进行更准确、可靠和有文件记录的核算。这是自时间因素开始起作用以来,开始积极讨论的调整国家税收制度以尽量减少公司损失的选择。这个问题的一个重要方面是,需要根据所选择的应对办法,同时和迅速地在综合设施的管制、税收、统计等领域采取措施。新措施不仅会影响国家的气候计划、税收和对外贸易,还会对经济战略甚至区域发展产生影响。
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引用次数: 0
Transformation of legal consciousness as a threat to the cybersecurity of an individual 法律意识的转变对个人网络安全的威胁
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236508003
Alexandra Brovkina, Vadim Burukin, Valentina Makarova, Tatiana Trifonova
The widespread availability of information and telecommunication sources (IT technologies), on the one hand, increases the level of legal awareness of the population and the effectiveness of interaction between political institutions and civil society, the activities of state bodies are becoming more transparent, education is more accessible. But in parallel with this, the level of the destructive impact of digitalization of public relations is also growing. A person dissolves into virtual reality, the personality is split into two, i.e. realized and manifested at different levels in different ways in virtual and real reality, there is a transformation of consciousness and, as a consequence of legal consciousness. In our opinion, the above provision is an urgent problem and requires theoretical and legal analysis. The article examines the problems of transformation of the legal consciousness of an individual in the digital space. The authors propose measures aimed at reducing the unfavorable factors affecting the distortion of legal consciousness.
一方面,信息和电信资源(信息技术)的广泛可用性提高了人民的法律意识水平和政治机构与民间社会之间互动的有效性,国家机构的活动变得更加透明,教育更容易获得。但与此同时,公共关系数字化的破坏性影响也在不断增强。一个人溶入虚拟现实,人格分裂为两个,即在虚拟现实和现实现实中以不同的方式在不同的层次上实现和表现,这是一种意识的转化,作为法律意识的结果。我们认为,上述规定是一个亟待解决的问题,需要进行理论和法律分析。本文探讨了数字空间中个人法律意识的转化问题。笔者提出了减少影响法律意识扭曲的不利因素的措施。
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引用次数: 0
The role of timely diagnosis and treatment of ENT pathologies in maintaining the physical health of patients 及时诊断和治疗耳鼻喉科病理在维护患者身体健康中的作用
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236505002
A. Zolotar, A. Malevanets, E. Meteleva, S. Mustafaeva, A. Timan, E. Farzalyeva, S. Avkaeva, V. Serdtseva
The presented work analyzes the need for specialized diagnostic procedures in order to identify the presence of certain ENT pathologies in the patient, since their untimely detection and, as a result, treatment directly affect the physical health of a person as a whole. It is noted that a decrease in patients’ attention to their health, as well as an untimely appeal to a specialized specialist for advice, can lead to the development of serious complications and even surgical intervention. The authors investigate in detail the features of common ENT diseases, possible symptoms, as well as complications that arise in the case of a chronic course of such diseases. At the same time, considerable emphasis is placed on the importance of the prevention of ENT diseases, among these measures are proper nutrition, a healthy lifestyle, regular medical examinations and following recommendations for the care of ENT organs. The paper emphasizes that timely diagnosis and treatment of ENT pathologies should be identified as key factors that ensure the necessary level of physical health of patients, for this reason, it is necessary to consult a specialist at the first symptoms of the disease. Investigating the methods of diagnosis of ENT diseases, the authors of the work also pay attention to the consideration of methods of treatment of ENT diseases, especially noting conservative therapy, since at the early stages of the development of the disease it allows to restore the patient’s health without resorting to surgical intervention. Based on the results of consideration of the problem, it should be concluded that timely referral to a specialist, diagnosis and treatment of ENT diseases are necessary conditions for maintaining and improving the physical health of patients and their quality of life.
所介绍的工作分析了需要专门的诊断程序,以确定患者是否存在某些耳鼻喉科病理,因为它们的不及时发现和治疗直接影响到整个人的身体健康。报告指出,患者对自己健康的关注减少,以及不及时地向专科医生寻求咨询,可能导致严重并发症的发生,甚至进行手术干预。作者详细调查了常见的耳鼻喉科疾病的特点,可能的症状,以及在这种疾病的慢性病程中出现的并发症。同时,相当重视预防耳鼻喉科疾病的重要性,这些措施包括适当的营养、健康的生活方式、定期体检和以下护理耳鼻喉科器官的建议。本文强调,及时诊断和治疗耳鼻喉科病理应确定为关键因素,以确保患者的身体健康的必要水平,因此,有必要咨询专家在疾病的第一个症状。在研究耳鼻喉科疾病的诊断方法时,作者还注意到耳鼻喉科疾病的治疗方法,特别是注意到保守疗法,因为在疾病发展的早期阶段,它可以恢复患者的健康,而无需诉诸手术干预。根据问题的考虑结果,应得出结论,及时转诊专科,诊断和治疗耳鼻喉科疾病是维持和改善患者身体健康和生活质量的必要条件。
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引用次数: 0
Cytotoxicity of some preservatives for culture Chlorella Vulgaris GKO strain 几种防腐剂对小球藻GKO菌株的细胞毒性研究
Pub Date : 2023-01-01 DOI: 10.1051/bioconf/20236605008
O. Azhikina, I. Makhnin, Yu. Berenev, L.Yu. Karpenko
One of the ways to preserve the culture of microalgae for a long time is conservation. All modern methods of preservation and long-term storage of microorganism cultures are based on the transfer of cells to a state of anabiosis, in which metabolic processes are either completely or partially stopped. One of the mechanisms that are realized during the transition to the anabiotic state is a change in cell permeability. This can be achieved by modifying the culture medium. Thus, the search for preservatives that contribute to the long-term preservation of biomass is very relevant. The primary condition for the use of any preservative or a new component of the culture medium should be the absence of a toxic effect on the cell culture. Goal of the work: to determine the cytotoxic effect of some preservatives for the cell culture of Chlorella vulgaris GKO strain. The cytotoxicity of ascorbic acid, lactic acid, citric acid, acetic acid, sodium chloride and urotropin was analyzed for the cell culture of Chlorella vulgaris strain GKO. The following parameters were determined: pH of Chlorella vulgaris suspension with preservative; Total number of cells, MM/ml; The ratio of dead cells to the total number of cells,%; Specific growth/death rate; The optical density of the suspension; The difference in the average optical density,%; Cell size (diameter), µm pH of suspension of Chlorella vulgaris with preservative; Total number of cells, MM/ml; The ratio of dead cells to the total number of cells,%; Specific growth/death rate; The optical density of the suspension; The difference in the average optical density,%; Cell size (diameter), µm pH of suspension of Chlorella vulgaris with preservative; Total number of cells, MM/ml; The ratio of dead cells to the total number of cells,%; Specific growth/death rate; The optical density of the suspension; The difference in the average optical density,%; Cell size (diameter), µm
长期保存微藻培养物的方法之一是保存。所有保存和长期储存微生物培养物的现代方法都是基于将细胞转移到复苏状态,在这种状态下,代谢过程要么完全停止,要么部分停止。在向厌氧状态过渡的过程中实现的机制之一是细胞通透性的改变。这可以通过修改培养基来实现。因此,寻找有助于生物质长期保存的防腐剂是非常重要的。使用任何防腐剂或培养基的新成分的首要条件应该是对细胞培养没有毒性作用。目的:测定几种防腐剂对普通小球藻GKO菌株细胞培养的细胞毒作用。研究了抗坏血酸、乳酸、柠檬酸、乙酸、氯化钠和尿激酶对普通小球藻GKO的细胞毒性。测定了以下参数:添加防腐剂的小球藻悬浮液的pH值;细胞总数,MM/ml;死亡细胞占细胞总数的比例,%;特定生长/死亡率;悬浮液的光密度;平均光密度之差,%;细胞大小(直径),添加防腐剂的普通小球藻悬浮液的µm pH;细胞总数,MM/ml;死亡细胞占细胞总数的比例,%;特定生长/死亡率;悬浮液的光密度;平均光密度之差,%;细胞大小(直径),添加防腐剂的普通小球藻悬浮液的µm pH;细胞总数,MM/ml;死亡细胞占细胞总数的比例,%;特定生长/死亡率;悬浮液的光密度;平均光密度之差,%;细胞尺寸(直径),µm
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引用次数: 0
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