Pub Date : 2023-01-01DOI: 10.1051/bioconf/20236307011
S. K. Cherchesova, M. I. Shapovalov, V. I. Mamaev, I. E. Dzhioeva, A. Yakimov
Data on the degree of knowledge on different groups of aquatic invertebrates on northern slopes of the Central Caucasus are given. It is shown that the best known groups are mayflies, stoneflies, odonates and caddisflies. Other groups have been studied to a much smaller degree. The estimated number of species in the study area is several hundreds. Secondary aquatic insect groups, such as those of the order Diptera, are the most speciose. Data on the degree of study of various groups of aquatic invertebrates on the northern slopes of the Central Caucasus are presented. It is shown that the most studied groups are mayflies, stoneflies, dragonflies and caddisflies. Other groups have been studied to a much lesser extent. The estimated number of species in the study area is several hundred. Minor groups of aquatic insects, such as the order Diptera, are more diverse.
{"title":"Freshwater Fauna of Invertebrates of the Northern Slopes of the Central Caucasus","authors":"S. K. Cherchesova, M. I. Shapovalov, V. I. Mamaev, I. E. Dzhioeva, A. Yakimov","doi":"10.1051/bioconf/20236307011","DOIUrl":"https://doi.org/10.1051/bioconf/20236307011","url":null,"abstract":"Data on the degree of knowledge on different groups of aquatic invertebrates on northern slopes of the Central Caucasus are given. It is shown that the best known groups are mayflies, stoneflies, odonates and caddisflies. Other groups have been studied to a much smaller degree. The estimated number of species in the study area is several hundreds. Secondary aquatic insect groups, such as those of the order Diptera, are the most speciose. Data on the degree of study of various groups of aquatic invertebrates on the northern slopes of the Central Caucasus are presented. It is shown that the most studied groups are mayflies, stoneflies, dragonflies and caddisflies. Other groups have been studied to a much lesser extent. The estimated number of species in the study area is several hundred. Minor groups of aquatic insects, such as the order Diptera, are more diverse.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87161282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1051/bioconf/20235709001
Nikita Lyakhovchenko, Elizabeth Gubina, V. Senchenkov, Ilya A. Nikishin, I. Solyanikova
The study presents growth kinetics of a BG28 bacterial strain isolated from vermicompost (generated with the use of Eisenia fetida worms). The strain was given a codename BG28. It was revealed that the isolate is capable of using high concentrations of sodium benzoate (up to 15 g/l) as a substrate. At the same time, the rate and division constants of a culture that grows at 5 g/l, 10 g/l, and 15 g/l do not differ. However, the mean and specific increment of BG28 at the end of the logarithmic growth phase is significantly higher in the variant with 5 g/l. It was testified, that with the shortest duration of the logarithmic growth phase on a medium with 5 g/l, the substrate loss constant is higher than in other variants. The difference in the kinetic parameters of the culture at 5 g/l and 10 g/l sodium benzoate is insignificant. With an increase of the substrate concentration to 15 g/l, the duration of the logarithmic growth phase increased significantly, but the sodium benzoate decrease constant was found to be the lowest. During the study of the individual properties of BG28, it was revealed that the strain is capable of growing on benzoic acid. On this basis, it can be assumed that the culture contributes to the degradation of plant residues during vermicomposting. Besides, the isolate grows on a mineral nutrient medium with polyethylene glycol 6000 and liquid paraffin. In the process of the individual properties estimation, it was revealed that the strain is capable of local suppression of the Alternaria brassicicola VKM F-1864 mold growth when co-cultivated on agar nutrient medium.
{"title":"Estimation of the Ability to Decompose Sodium Benzoate by a Bacterium Isolated from Biohumus Eicenia Fetida","authors":"Nikita Lyakhovchenko, Elizabeth Gubina, V. Senchenkov, Ilya A. Nikishin, I. Solyanikova","doi":"10.1051/bioconf/20235709001","DOIUrl":"https://doi.org/10.1051/bioconf/20235709001","url":null,"abstract":"The study presents growth kinetics of a BG28 bacterial strain isolated from vermicompost (generated with the use of Eisenia fetida worms). The strain was given a codename BG28. It was revealed that the isolate is capable of using high concentrations of sodium benzoate (up to 15 g/l) as a substrate. At the same time, the rate and division constants of a culture that grows at 5 g/l, 10 g/l, and 15 g/l do not differ. However, the mean and specific increment of BG28 at the end of the logarithmic growth phase is significantly higher in the variant with 5 g/l. It was testified, that with the shortest duration of the logarithmic growth phase on a medium with 5 g/l, the substrate loss constant is higher than in other variants. The difference in the kinetic parameters of the culture at 5 g/l and 10 g/l sodium benzoate is insignificant. With an increase of the substrate concentration to 15 g/l, the duration of the logarithmic growth phase increased significantly, but the sodium benzoate decrease constant was found to be the lowest. During the study of the individual properties of BG28, it was revealed that the strain is capable of growing on benzoic acid. On this basis, it can be assumed that the culture contributes to the degradation of plant residues during vermicomposting. Besides, the isolate grows on a mineral nutrient medium with polyethylene glycol 6000 and liquid paraffin. In the process of the individual properties estimation, it was revealed that the strain is capable of local suppression of the Alternaria brassicicola VKM F-1864 mold growth when co-cultivated on agar nutrient medium.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87765603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1051/bioconf/20236501001
Lyudmila Kravchenko, Yevgeniy Chayka, Viktor Zhurba
An important generally recognized factor in improving the quality of cultivation in recent years is the elastic fastening of working organs. It is believed that the working bodies fixed with elastic struts make intense vibrations, which leads to an improvement in the quality of soil crumbling and a decrease in traction resistance similar to the vibrations of a vibrator. However, the paws on the elastic rack when working receive a deviation from the set processing depth by 10-15% more than with a rigid suspension and do not maintain the optimal cutting geometry. It is established that elastic displacements depend on the configuration of spring struts.
{"title":"Optimization of the parameters of the spring rack of the cultivator","authors":"Lyudmila Kravchenko, Yevgeniy Chayka, Viktor Zhurba","doi":"10.1051/bioconf/20236501001","DOIUrl":"https://doi.org/10.1051/bioconf/20236501001","url":null,"abstract":"An important generally recognized factor in improving the quality of cultivation in recent years is the elastic fastening of working organs. It is believed that the working bodies fixed with elastic struts make intense vibrations, which leads to an improvement in the quality of soil crumbling and a decrease in traction resistance similar to the vibrations of a vibrator. However, the paws on the elastic rack when working receive a deviation from the set processing depth by 10-15% more than with a rigid suspension and do not maintain the optimal cutting geometry. It is established that elastic displacements depend on the configuration of spring struts.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88335170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
There are conflicting views among researchers on the precise beginning of champignon cultivation. While some contend that its cultivation began in France, others assert that it originated in Italy around a thousand years ago. French farmers discovered in the 17th century that champignons thrived not just on manure-fertilized lawns but also in dark, damp quarters, a method that is still used today. Champignon was cultivated on dedicated farms on royal estates when European kings ruled the continent because it was a costly and uncommon delicacy. The objective of this study was to analyze the composition of the common mushroom using mass spectrometry with a Chromatek-Crystal 5000 spectrometer. The study aimed to extract and quantitatively determine the compounds present in the common fungus. A technology for obtaining the drug was developed through experiments, which involved extracting plant materials with absolute alcohol. The methodology employed can be used to further explore the properties of the common mushroom, and contribute to the development of effective pharmaceutical products.
{"title":"Chromato-mass-spectrometry of the analysis of the sum of the common mushrooms","authors":"Bairamdurdi Sapaev, Foziljon Saitkulov, Muattar Mamedova, Shahlo Saydaliyeva, Dilafruz Makhmudova","doi":"10.1051/bioconf/20236501006","DOIUrl":"https://doi.org/10.1051/bioconf/20236501006","url":null,"abstract":"There are conflicting views among researchers on the precise beginning of champignon cultivation. While some contend that its cultivation began in France, others assert that it originated in Italy around a thousand years ago. French farmers discovered in the 17th century that champignons thrived not just on manure-fertilized lawns but also in dark, damp quarters, a method that is still used today. Champignon was cultivated on dedicated farms on royal estates when European kings ruled the continent because it was a costly and uncommon delicacy. The objective of this study was to analyze the composition of the common mushroom using mass spectrometry with a Chromatek-Crystal 5000 spectrometer. The study aimed to extract and quantitatively determine the compounds present in the common fungus. A technology for obtaining the drug was developed through experiments, which involved extracting plant materials with absolute alcohol. The methodology employed can be used to further explore the properties of the common mushroom, and contribute to the development of effective pharmaceutical products.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88431225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1051/bioconf/20236308002
S. Tsutsulaeva, Eliza Yangulbaeva
The purpose of the article is to identify the mechanisms of reproduction and transformation of the mental and behavioral aspects of traditional ethno-social practices in extreme conditions of exile and ethnic deportation of the Chechen people.The question of the influence of the geographical environment on the socio-political processes and mores of society was raised by ancient authors, but the great geographical discoveries that caused the rapid economic and socio-political development of Europe served as the historical prerequisites for the emergence of an integral concept of geographical determinism.
{"title":"Influence of the Natural and Geographical Environment on the Adaptation of the Deported People During the Great Patriotic War (on the Example of the Chechens)","authors":"S. Tsutsulaeva, Eliza Yangulbaeva","doi":"10.1051/bioconf/20236308002","DOIUrl":"https://doi.org/10.1051/bioconf/20236308002","url":null,"abstract":"The purpose of the article is to identify the mechanisms of reproduction and transformation of the mental and behavioral aspects of traditional ethno-social practices in extreme conditions of exile and ethnic deportation of the Chechen people.The question of the influence of the geographical environment on the socio-political processes and mores of society was raised by ancient authors, but the great geographical discoveries that caused the rapid economic and socio-political development of Europe served as the historical prerequisites for the emergence of an integral concept of geographical determinism.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86110276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1051/bioconf/20235903016
Jianhe Liu, Wenzheng Li
The study of aerosol deposition in the human upper respiratory tract is of great significance for understanding the impact of toxic aerosols on human health and improving the therapeutic effect of drug aerosol inhalation. 3D printing technology was used in the experimental model of the human upper airway. A laser particle size analyzer was used to study the deposition of aerosol particles with a particle size of 1-20 μm inhaled in the upper respiratory tract of the human body, and the particle deposition rules of the aerosol particles in the upper respiratory tract were analyzed. The experimental results show that aerosol particles remain more in the pharynx, larynx and position. For particles with a particle size of 1-5 μm, more deposition will occur in the trachea, and for particles with a particle size of 10-20 μm, most of them will be deposited in the mouth.
{"title":"Experimental study on aerosol inhalation of the human upper airway","authors":"Jianhe Liu, Wenzheng Li","doi":"10.1051/bioconf/20235903016","DOIUrl":"https://doi.org/10.1051/bioconf/20235903016","url":null,"abstract":"The study of aerosol deposition in the human upper respiratory tract is of great significance for understanding the impact of toxic aerosols on human health and improving the therapeutic effect of drug aerosol inhalation. 3D printing technology was used in the experimental model of the human upper airway. A laser particle size analyzer was used to study the deposition of aerosol particles with a particle size of 1-20 μm inhaled in the upper respiratory tract of the human body, and the particle deposition rules of the aerosol particles in the upper respiratory tract were analyzed. The experimental results show that aerosol particles remain more in the pharynx, larynx and position. For particles with a particle size of 1-5 μm, more deposition will occur in the trachea, and for particles with a particle size of 10-20 μm, most of them will be deposited in the mouth.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86183525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1051/bioconf/20235703004
A. Byakov, M. Karpov, N. Strizhov, M. Donova
The fast-growing saprotrophic strain Mycolicibacterium smegmatis mc2155 is capable of utilizing plant and animal sterols and can be used for creation of genetically engineered strains producing biologically active steroids. Oxidation of the 3β-hydroxyl group and Δ5(6)→Δ4(5) double bond isomerization followed by formation of stenones from sterols are considered as the initial stage of steroid catabolism in some actinobacteria. The study of the mechanism of steroid nucleus 3β-hydroxyl group oxidation is relevant for the creation of a method of the microbiological production of valuable 3β-hydroxy-5-en-steroids. A mutant strain of M. smegmatis with deletions in three genes (MSMEG_1604, MSMEG_5228 and MSMEG_5233) encoding known enzymes exhibiting 3β-hydroxysteroid dehydrogenase activity was constructed by homologous recombination coupled with double selection. The resulting mutant retained macromorphological properties and the ability to convert cholesterol. 3-Keto-4-en-steroids were found among the sterol catabolism intermediates. Experimentally obtained data indicate the presence of a previously undetected intracellular enzyme that performs the function of 3β-hydroxysteroid dehydrogenase/Δ5(6)→Δ4(5) isomerase.
{"title":"Creation and Characterization of Mycolicibacterium Smegmatis mc2155 with Deletions in Genes Encoding Sterol Oxidation Enzymes","authors":"A. Byakov, M. Karpov, N. Strizhov, M. Donova","doi":"10.1051/bioconf/20235703004","DOIUrl":"https://doi.org/10.1051/bioconf/20235703004","url":null,"abstract":"The fast-growing saprotrophic strain Mycolicibacterium smegmatis mc2155 is capable of utilizing plant and animal sterols and can be used for creation of genetically engineered strains producing biologically active steroids. Oxidation of the 3β-hydroxyl group and Δ5(6)→Δ4(5) double bond isomerization followed by formation of stenones from sterols are considered as the initial stage of steroid catabolism in some actinobacteria. The study of the mechanism of steroid nucleus 3β-hydroxyl group oxidation is relevant for the creation of a method of the microbiological production of valuable 3β-hydroxy-5-en-steroids. A mutant strain of M. smegmatis with deletions in three genes (MSMEG_1604, MSMEG_5228 and MSMEG_5233) encoding known enzymes exhibiting 3β-hydroxysteroid dehydrogenase activity was constructed by homologous recombination coupled with double selection. The resulting mutant retained macromorphological properties and the ability to convert cholesterol. 3-Keto-4-en-steroids were found among the sterol catabolism intermediates. Experimentally obtained data indicate the presence of a previously undetected intracellular enzyme that performs the function of 3β-hydroxysteroid dehydrogenase/Δ5(6)→Δ4(5) isomerase.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86431658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This essay establishes three factors, i.e. organic fertilizer, chemical fertilizer and organic fertilizer with cow carbide. The purpose of the present study was to elucidate influence of organic fertilizer and chemical fertilizer on yield and inner quality in spinach with fifteen treatments at five levels. The results showed that the contents of reducing sugar and nitrate nitrogen of spinach in organic fertilizer handling area was less than those in chemical fertilizer. No significant difference was found among the fresh weight, total sugar content and vitamin C content by utilization of organic fertilizer or chemical fertilizer.
{"title":"Comparison of the Influence of Organic and Conventional Cultivation on Yield and Inner Quality in Spinach (Spinacia Oleracea L.)","authors":"Jinhua Bao, Cheng Wang, Xiangjun Shen, Defeng Zhuang, Eriko Sasakid","doi":"10.1051/bioconf/20236002001","DOIUrl":"https://doi.org/10.1051/bioconf/20236002001","url":null,"abstract":"This essay establishes three factors, i.e. organic fertilizer, chemical fertilizer and organic fertilizer with cow carbide. The purpose of the present study was to elucidate influence of organic fertilizer and chemical fertilizer on yield and inner quality in spinach with fifteen treatments at five levels. The results showed that the contents of reducing sugar and nitrate nitrogen of spinach in organic fertilizer handling area was less than those in chemical fertilizer. No significant difference was found among the fresh weight, total sugar content and vitamin C content by utilization of organic fertilizer or chemical fertilizer.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82942152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1051/bioconf/20236506005
Hasanboy Abdusamatov
The article is devoted to socio-psychological characteristics of children in migrant families, and reveals their problems. It presents the research results of foreign and Uzbek scientists who conducted research on migrant families and their children. The article proves that growing up without a father or mother has a negative effect on the child’s psychology, and that family problems arise as a result of the increase in the number of migrants in the world, as a result of which family members do not see each other for a long time. The conflict in personal and family values, its impact on the upbringing system of Uzbek families, as well as methods of bringing up and educating the children of migrant families and providing psychological services to them in educational institutions are shown. Psychological recommendations are given to parents who have left their children in their homeland and become migrant workers in other countries.
{"title":"Socio-psychological problems of children in migrant families and their solutions","authors":"Hasanboy Abdusamatov","doi":"10.1051/bioconf/20236506005","DOIUrl":"https://doi.org/10.1051/bioconf/20236506005","url":null,"abstract":"The article is devoted to socio-psychological characteristics of children in migrant families, and reveals their problems. It presents the research results of foreign and Uzbek scientists who conducted research on migrant families and their children. The article proves that growing up without a father or mother has a negative effect on the child’s psychology, and that family problems arise as a result of the increase in the number of migrants in the world, as a result of which family members do not see each other for a long time. The conflict in personal and family values, its impact on the upbringing system of Uzbek families, as well as methods of bringing up and educating the children of migrant families and providing psychological services to them in educational institutions are shown. Psychological recommendations are given to parents who have left their children in their homeland and become migrant workers in other countries.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86718847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to explore the effect of treadmill exercise on anxiety in rats. Thirty SPF male rats aged 2 months with a body mass of (225±25) g were randomly divided into control group (CG, n=10), chronic sleep deprivation group (CSD, n=10) and sleep deprivation exercise group (CSD+E, n=10) after adaptive feeding for 1 week. The CSD model of rats in CSD group and CSD+E group was made by multi platform water environment method. Sleep deprivation of 18 h per day (from 12:00 pm. to 6:00 am. the next day) for 8 weeks. The effect of running on the anxiety-like behaviour of CSD rats was examined in the open field test (OFT) and the elevated plus maze (EPM) experiment. Hematoxylin-eosin (HE) staining, Annexin V/PI flow cytometry, immunofluorescence staining, Western blot, RT-qPCR and other methods were used to detect the effects of treadmill exercise on the morphology of hippocampus, apoptosis related factors caspase-12, Bax, Bcl-1, inflammatory factors (IL-6, TNF-a), Wnt β- catenin, p- β-catenin. The results of HE staining showed that the brain tissue of the control rats was structurally intact, with thick layers of cone cells, relatively dense, neatly arranged and compact, the cell edge structures were intact and clearly visible, with no obvious abnormal changes. The cone cell layer of brain tissue in the chronic sleep deprivation group was thin, with relatively low cell density, disorganized and sparse arrangement, and blurred cell edges. The symptoms of the above pathological changes in brain tissue of rats in sleep deprivation exercise group gradually alleviated. OFT results showed that compared with CG group, the number of activities in the central region of CSD group was significantly reduced (P<0.01), and the total distance of exercise was significantly shortened (P<0.01). EPM results showed that compared with CG group, OT and CE in CSD group decreased significantly (P<0.01). CCK-8 results showed that compared with CG group, the activity of neurons in CA1 area of hippocampus in CSD group was significantly decreased (P<0.01), while that in CSD+E group was significantly increased (P<0.01); Annexin V/PI flow cytometry results showed that compared with CG group, the apoptosis of neurons in CA1 area of hippocampus in CSD group increased (P<0.01), and that in CSD+E group decreased significantly (P<0.01);Western blot results showed that caspase-12, Bax, IL-6, IL-1β and TNF-a were highly expressed and Bcl-1 was lowly expressed in hippocampal tissues of rats in the CSD group compared with the CG group (P<0.01), Wnt, β-catenin and p-β-catenin were lowly expressed in hippocampal tissues of rats in the CSD group, and Gsk-3βprotein expression was significantly higher (p<0.01). The results of RT-qPCR showed that caspase-12 mRNA, Bax mRNA, IL-6 mRNA, TNF-a mRNA and IL-1β mRNA were highly expressed and Bcl-1 mRNA was lowly expressed in hippocampal tissues of rats in the CSD group compared with the CG group (P<0.01). Compared with the CG group, Wnt mRNA and β
{"title":"Mechanisms by Which Physical Activity Modulates the Wnt/β-catenin Pathway to Alleviate Anxiety-like Depression","authors":"Xiaofeng Jiang, Ziwei Ni, Q. Feng, Hongtao Guo, D. Fu, Junmian Wang, Hongtao Zhou, Xuefeng Liang, Cailian Ruan","doi":"10.1051/bioconf/20236101005","DOIUrl":"https://doi.org/10.1051/bioconf/20236101005","url":null,"abstract":"This study aimed to explore the effect of treadmill exercise on anxiety in rats. Thirty SPF male rats aged 2 months with a body mass of (225±25) g were randomly divided into control group (CG, n=10), chronic sleep deprivation group (CSD, n=10) and sleep deprivation exercise group (CSD+E, n=10) after adaptive feeding for 1 week. The CSD model of rats in CSD group and CSD+E group was made by multi platform water environment method. Sleep deprivation of 18 h per day (from 12:00 pm. to 6:00 am. the next day) for 8 weeks. The effect of running on the anxiety-like behaviour of CSD rats was examined in the open field test (OFT) and the elevated plus maze (EPM) experiment. Hematoxylin-eosin (HE) staining, Annexin V/PI flow cytometry, immunofluorescence staining, Western blot, RT-qPCR and other methods were used to detect the effects of treadmill exercise on the morphology of hippocampus, apoptosis related factors caspase-12, Bax, Bcl-1, inflammatory factors (IL-6, TNF-a), Wnt β- catenin, p- β-catenin. The results of HE staining showed that the brain tissue of the control rats was structurally intact, with thick layers of cone cells, relatively dense, neatly arranged and compact, the cell edge structures were intact and clearly visible, with no obvious abnormal changes. The cone cell layer of brain tissue in the chronic sleep deprivation group was thin, with relatively low cell density, disorganized and sparse arrangement, and blurred cell edges. The symptoms of the above pathological changes in brain tissue of rats in sleep deprivation exercise group gradually alleviated. OFT results showed that compared with CG group, the number of activities in the central region of CSD group was significantly reduced (P<0.01), and the total distance of exercise was significantly shortened (P<0.01). EPM results showed that compared with CG group, OT and CE in CSD group decreased significantly (P<0.01). CCK-8 results showed that compared with CG group, the activity of neurons in CA1 area of hippocampus in CSD group was significantly decreased (P<0.01), while that in CSD+E group was significantly increased (P<0.01); Annexin V/PI flow cytometry results showed that compared with CG group, the apoptosis of neurons in CA1 area of hippocampus in CSD group increased (P<0.01), and that in CSD+E group decreased significantly (P<0.01);Western blot results showed that caspase-12, Bax, IL-6, IL-1β and TNF-a were highly expressed and Bcl-1 was lowly expressed in hippocampal tissues of rats in the CSD group compared with the CG group (P<0.01), Wnt, β-catenin and p-β-catenin were lowly expressed in hippocampal tissues of rats in the CSD group, and Gsk-3βprotein expression was significantly higher (p<0.01). The results of RT-qPCR showed that caspase-12 mRNA, Bax mRNA, IL-6 mRNA, TNF-a mRNA and IL-1β mRNA were highly expressed and Bcl-1 mRNA was lowly expressed in hippocampal tissues of rats in the CSD group compared with the CG group (P<0.01). Compared with the CG group, Wnt mRNA and β","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91454260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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