Pub Date : 2021-11-29DOI: 10.31557/apjcb.2021.6.4.339-341
E. Nazar, E. Hashemi, S. Ahmadi, Keyvan Baloochi
Introduction: Nodular fasciitis is an exceptional benign soft tissue tumor of the breast that clinically and radiologically can simulate invasive duct carcinoma. Case presentation: We report a case of 67-year-old woman who had a palpable right breast mass of 2 months duration. Sonography showed a hypoechoic heterogeneous round nodule with irregular and speculated borders in right breast. Right breast mass core needle biopsy was inconclusive. Right mastectomy was done due to highly suspicious for malignancy on imaging. Pathological and immunohistochemical examination revealed that the tumor composed of a stellate spindle cell tumor with red blood cells extravasation, compatible with nodular fasciitis. Therefore, pathological evaluation of the lesion is crucial in the assembly of the diagnosis. Conclusion: Nodular fasciitis should be considered in the differential diagnosis of any suspicious breast mass.
{"title":"A Case of Nodular Fasciitis of the Breast Previously Misdiagnosed as Breast Carcinoma; A Case Report","authors":"E. Nazar, E. Hashemi, S. Ahmadi, Keyvan Baloochi","doi":"10.31557/apjcb.2021.6.4.339-341","DOIUrl":"https://doi.org/10.31557/apjcb.2021.6.4.339-341","url":null,"abstract":"Introduction: Nodular fasciitis is an exceptional benign soft tissue tumor of the breast that clinically and radiologically can simulate invasive duct carcinoma. Case presentation: We report a case of 67-year-old woman who had a palpable right breast mass of 2 months duration. Sonography showed a hypoechoic heterogeneous round nodule with irregular and speculated borders in right breast. Right breast mass core needle biopsy was inconclusive. Right mastectomy was done due to highly suspicious for malignancy on imaging. Pathological and immunohistochemical examination revealed that the tumor composed of a stellate spindle cell tumor with red blood cells extravasation, compatible with nodular fasciitis. Therefore, pathological evaluation of the lesion is crucial in the assembly of the diagnosis. Conclusion: Nodular fasciitis should be considered in the differential diagnosis of any suspicious breast mass.","PeriodicalId":8848,"journal":{"name":"Asian Pacific Journal of Cancer Biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89120794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-11DOI: 10.31557/apjcb.2021.6.3.225-227
P. Gadhia, Jessica Jeejan, Vishma Shah, M. Patel, S. Vaniawala
Background: BCR-ABL kinase domain(KD) mutations accounts for 60-80% of Imatinib resistance in chronic myeloid leukemia (CML) – chronic phase (CP). Patients with CML who are receiving imatinib treatment, a mutation analysis is required to find out the resistance of imatinib as per European Leukemia Net (ELN) criteria. The present study was carried out to assess for different types of mutations responsible for resistance of imatinib treatment from Western India. Methods: In a retrospective study, the patients who were tested for imatinib resistance were analysed for IRMA testing using direct sequencing of BCR-ABL transcript by Sanger method. Results: A total of 215 patients were tested for Imatinib resistance analysis (IRMA), of which 45 (20.93%) had detectable mutations. The highest frequency of mutation recorded at T315I amino acids site, followed by M244V and G250E sites. Conclusion: The patients who were tested for IRMA showed 20.93 % positive mutations with reference to its resistance are discussed.
{"title":"A Study on BCR-ABL Kinase Domain Mutations in Chronic Myeloid Leukemia from Western India","authors":"P. Gadhia, Jessica Jeejan, Vishma Shah, M. Patel, S. Vaniawala","doi":"10.31557/apjcb.2021.6.3.225-227","DOIUrl":"https://doi.org/10.31557/apjcb.2021.6.3.225-227","url":null,"abstract":"Background: BCR-ABL kinase domain(KD) mutations accounts for 60-80% of Imatinib resistance in chronic myeloid leukemia (CML) – chronic phase (CP). Patients with CML who are receiving imatinib treatment, a mutation analysis is required to find out the resistance of imatinib as per European Leukemia Net (ELN) criteria. The present study was carried out to assess for different types of mutations responsible for resistance of imatinib treatment from Western India. Methods: In a retrospective study, the patients who were tested for imatinib resistance were analysed for IRMA testing using direct sequencing of BCR-ABL transcript by Sanger method. Results: A total of 215 patients were tested for Imatinib resistance analysis (IRMA), of which 45 (20.93%) had detectable mutations. The highest frequency of mutation recorded at T315I amino acids site, followed by M244V and G250E sites. Conclusion: The patients who were tested for IRMA showed 20.93 % positive mutations with reference to its resistance are discussed. \u0000 ","PeriodicalId":8848,"journal":{"name":"Asian Pacific Journal of Cancer Biology","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81264411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-11DOI: 10.31557/apjcb.2021.6.3.219-224
Samira Molashahi, D. Farhud, M. Saffari, E. Ebrahimi, Amirnader Emamirazavi, R. Shirkoohi
Background: Fibroblast growth factor-10 (FGF-10) is a member of a superfamily with characteristics of epithelial cell proliferation and embryonic development and is assumed to have a role in a phenomenon called the epithelial-mesenchymal transition (EMT). The previous study has revealed the critical role of FGF-10 in type III EMT in breast cancer cell lines. The mentioned finding, demonstrates the possible role of this factor in type III EMT in cancers with different origins such as breast. The present study investigated the expression of FGF-10 as a mitotic-inducing growth factor, normally has a low expression in breast tissues amongst breast cancer patients. Materials and Methods: 67 breast cancer tissues and 8 normal breast tissues were randomly selected from the Iran national tumor bank. The FGF-10 gene expression analysis was performed after the RNA expression using the real-time RTPCR, which was followed by the Student’s t-test statistical analysis. Results: The findings revealed that the relative expression of FGF-10 was elevated in tumor tissues as compared with normal breast tissues, and the higher expression had a direct correlation with the progression of clinical and pathologic staging. The expression was also significantly higher in triple-negative breast cancer and p53 null tissues. Conclusion: Taken together, it is suggested that, although in some variables it was not significant but generally the invasion and migration in tumor tissues are the same as in-vitro analysis as indicated before, and the expression has a direct relationship with the molecular presentation and clinical -pathological progression.
{"title":"The Expression, Morphology, and Clinical Characteristics of Fibroblast Growth Factor-10 in Breast Cancer","authors":"Samira Molashahi, D. Farhud, M. Saffari, E. Ebrahimi, Amirnader Emamirazavi, R. Shirkoohi","doi":"10.31557/apjcb.2021.6.3.219-224","DOIUrl":"https://doi.org/10.31557/apjcb.2021.6.3.219-224","url":null,"abstract":"Background: Fibroblast growth factor-10 (FGF-10) is a member of a superfamily with characteristics of epithelial cell proliferation and embryonic development and is assumed to have a role in a phenomenon called the epithelial-mesenchymal transition (EMT). The previous study has revealed the critical role of FGF-10 in type III EMT in breast cancer cell lines. The mentioned finding, demonstrates the possible role of this factor in type III EMT in cancers with different origins such as breast. The present study investigated the expression of FGF-10 as a mitotic-inducing growth factor, normally has a low expression in breast tissues amongst breast cancer patients. Materials and Methods: 67 breast cancer tissues and 8 normal breast tissues were randomly selected from the Iran national tumor bank. The FGF-10 gene expression analysis was performed after the RNA expression using the real-time RTPCR, which was followed by the Student’s t-test statistical analysis. Results: The findings revealed that the relative expression of FGF-10 was elevated in tumor tissues as compared with normal breast tissues, and the higher expression had a direct correlation with the progression of clinical and pathologic staging. The expression was also significantly higher in triple-negative breast cancer and p53 null tissues. Conclusion: Taken together, it is suggested that, although in some variables it was not significant but generally the invasion and migration in tumor tissues are the same as in-vitro analysis as indicated before, and the expression has a direct relationship with the molecular presentation and clinical -pathological progression. ","PeriodicalId":8848,"journal":{"name":"Asian Pacific Journal of Cancer Biology","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81356425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-22DOI: 10.31557/apjcb.2021.6.3.213-217
M. Hosen, Narwana Khaleque, Sajib Chakraborty, M. Mahtab, Y. Kabir
Background: Hepatocellular carcinoma (HCC) is one of the fatal cancer types worldwide, and a variety of genetic factors are considered to be associated with this incidence. MDM2 gene plays a pivotal role in various pathways, which are essential to combat tumor formation. The study aimed to find out the associations of MDM2 (T309G, rs2279744) gene polymorphism with the development of HCC in the Bangladeshi population. Methods: A case-control study on 100 HCC patients and 110 control subjects was conducted. The genotyping of the MDM2 (T309G) gene was done using PCR-RFLP methods. Results: The percentage of TT and GG genotypes were significantly different (p<0.01) among the study subjects. There were four genotyping groups, while the subjects with TT genotypes were considered the reference group. Patients with GG genotypes were at high risk of developing HCC (OR, 3.6; 95 % CI, 1.64–7.80; p<0.01) compared to the control. On the other hand, the association of TG genotypes with HCC was not statistically significant (OR, 1.8; 95 % CI, 0.91–3.40, p>0.05). In addition, patients having either GG or TG genotypes showed higher risk for HCC compared to control group (OR = 2.20; 95% CI = 1.21–4.14; P < 0.05). Conclusion: Our study suggested that the MDM2 gene may have a strong association with the development of HCC, and the GG allele could serve as an essential determinant to identify the higher risk of HCC in the Bangladeshi population.
{"title":"MDM2 (T309G) Gene Polymorphism Determines the Susceptibility of Hepatocellular Carcinoma in Bangladesh","authors":"M. Hosen, Narwana Khaleque, Sajib Chakraborty, M. Mahtab, Y. Kabir","doi":"10.31557/apjcb.2021.6.3.213-217","DOIUrl":"https://doi.org/10.31557/apjcb.2021.6.3.213-217","url":null,"abstract":"Background: Hepatocellular carcinoma (HCC) is one of the fatal cancer types worldwide, and a variety of genetic factors are considered to be associated with this incidence. MDM2 gene plays a pivotal role in various pathways, which are essential to combat tumor formation. The study aimed to find out the associations of MDM2 (T309G, rs2279744) gene polymorphism with the development of HCC in the Bangladeshi population. Methods: A case-control study on 100 HCC patients and 110 control subjects was conducted. The genotyping of the MDM2 (T309G) gene was done using PCR-RFLP methods. Results: The percentage of TT and GG genotypes were significantly different (p<0.01) among the study subjects. There were four genotyping groups, while the subjects with TT genotypes were considered the reference group. Patients with GG genotypes were at high risk of developing HCC (OR, 3.6; 95 % CI, 1.64–7.80; p<0.01) compared to the control. On the other hand, the association of TG genotypes with HCC was not statistically significant (OR, 1.8; 95 % CI, 0.91–3.40, p>0.05). In addition, patients having either GG or TG genotypes showed higher risk for HCC compared to control group (OR = 2.20; 95% CI = 1.21–4.14; P < 0.05). Conclusion: Our study suggested that the MDM2 gene may have a strong association with the development of HCC, and the GG allele could serve as an essential determinant to identify the higher risk of HCC in the Bangladeshi population.","PeriodicalId":8848,"journal":{"name":"Asian Pacific Journal of Cancer Biology","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85845261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-08DOI: 10.31557/apjcb.2021.6.3.207-212
Sumali Randhini Sumithrarachchi, W.A.Udari.C. Pemasiri, A. Pathiranage, P. Jayasooriya
Introduction: Oral cancer and Oral Potentially Malignant Disorders (OPMDs) arising due to risk habits like betel quid (BQ) chewing, smoking and alcohol use are preventable despite the high prevalence and poor survival rates. However, dependence to risk habits has become a barrier to prevent OPMDs and oral cancer. Objectives: This study was conducted to determine the dependence to risk habits among patients with such oral lesions, and the quitting among dependent users after diagnosis. Materials and method: A case-control study was conducted among 100 participants, 50 diagnosed with OPMDs and oral cancer from two Oral and Maxillo Facial clinics in Sri Lanka and a similar control group without any oral lesions. All participants practiced one or more risk habits such as BQ chewing, smoking and/or alcohol use. Dependence was assessed using Sinhala language translations of standardized scales such as Betel Quid Dependence Scale for BQ chewers, Fagerstrom Test for Nicotine Dependence for tobacco smokers and CAGE (“Cut down”, “Annoyed”, “Guilty” and “Eye opener”) scale for alcohol users. Results: Dependence on habits was more among the case group (BQ-93%; smoking-14%; alcohol-66%). Quitting rates of BQ and alcohol among those who were diagnosed with oral cancer [BQ (89%), alcohol (89%)] were higher (p<0.05) than those with OPMDs [BQ (50%), alcohol (22%)]. Lack of awareness due to low socio-economic status (97%) was a significant association for BQ dependence (p=0.01).Conclusion: In conclusion, development of OPMDs and oral cancer increases with dependence to BQ chewing, smoking and alcohol use. Increased awareness about OPMDs and its risk of malignancy is needed among public. Prevention of dependence to these risk habits also becomes essential.
{"title":"Betel Quid, Smoking and Alcohol Dependency among Patients with Oral Potentially Malignant Disorders and Oral Cancer in Sri Lanka; A Preliminary Case-control Study","authors":"Sumali Randhini Sumithrarachchi, W.A.Udari.C. Pemasiri, A. Pathiranage, P. Jayasooriya","doi":"10.31557/apjcb.2021.6.3.207-212","DOIUrl":"https://doi.org/10.31557/apjcb.2021.6.3.207-212","url":null,"abstract":"Introduction: Oral cancer and Oral Potentially Malignant Disorders (OPMDs) arising due to risk habits like betel quid (BQ) chewing, smoking and alcohol use are preventable despite the high prevalence and poor survival rates. However, dependence to risk habits has become a barrier to prevent OPMDs and oral cancer. Objectives: This study was conducted to determine the dependence to risk habits among patients with such oral lesions, and the quitting among dependent users after diagnosis. Materials and method: A case-control study was conducted among 100 participants, 50 diagnosed with OPMDs and oral cancer from two Oral and Maxillo Facial clinics in Sri Lanka and a similar control group without any oral lesions. All participants practiced one or more risk habits such as BQ chewing, smoking and/or alcohol use. Dependence was assessed using Sinhala language translations of standardized scales such as Betel Quid Dependence Scale for BQ chewers, Fagerstrom Test for Nicotine Dependence for tobacco smokers and CAGE (“Cut down”, “Annoyed”, “Guilty” and “Eye opener”) scale for alcohol users. Results: Dependence on habits was more among the case group (BQ-93%; smoking-14%; alcohol-66%). Quitting rates of BQ and alcohol among those who were diagnosed with oral cancer [BQ (89%), alcohol (89%)] were higher (p<0.05) than those with OPMDs [BQ (50%), alcohol (22%)]. Lack of awareness due to low socio-economic status (97%) was a significant association for BQ dependence (p=0.01).Conclusion: In conclusion, development of OPMDs and oral cancer increases with dependence to BQ chewing, smoking and alcohol use. Increased awareness about OPMDs and its risk of malignancy is needed among public. Prevention of dependence to these risk habits also becomes essential. \u0000 ","PeriodicalId":8848,"journal":{"name":"Asian Pacific Journal of Cancer Biology","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81986243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-08DOI: 10.31557/apjcb.2021.6.3.231-233
H. Tabriz, E. Nazar, Fatemeh Jazayeri, A. Javadi
Introduction: COVID-19 infection prompts inflammatory responses and acute lung injury in human beings. Complete blood count with differential is essential investigative tool in its managing. However, very few studies revealed the variations of blood cell morphology in this disease.Case report: We reported a 39-years- old female patient complained of respiratory distress one week prior to hospitalization. The patient suffered from cough, fever, and molecular test was reported positive for COVID-19 infection. Laboratory data revealed severe permanent leukopenia and peripheral blood smear examination showed blastoid cells after remission of respiratory signs. Patient underwent bone marrow biopsy for rule out acute myeloid leukemia. But, on bone marrow sample, only viral cytopathic effects were seen. COVID-19 virus stimulates inflammatory cells to produces various inflammatory cytokines and as a result, viral cytopathic effects on white blood cells is seen. Conclusion: We have described how the characteristic peripheral blood findings of COVID‐19 infection can be misinterpret as acute myeloid leukemia.
{"title":"Hematologic Presentations of COVID-19 Can be Misinterpreted as Acute Myeloid Leukemia","authors":"H. Tabriz, E. Nazar, Fatemeh Jazayeri, A. Javadi","doi":"10.31557/apjcb.2021.6.3.231-233","DOIUrl":"https://doi.org/10.31557/apjcb.2021.6.3.231-233","url":null,"abstract":"Introduction: COVID-19 infection prompts inflammatory responses and acute lung injury in human beings. Complete blood count with differential is essential investigative tool in its managing. However, very few studies revealed the variations of blood cell morphology in this disease.Case report: We reported a 39-years- old female patient complained of respiratory distress one week prior to hospitalization. The patient suffered from cough, fever, and molecular test was reported positive for COVID-19 infection. Laboratory data revealed severe permanent leukopenia and peripheral blood smear examination showed blastoid cells after remission of respiratory signs. Patient underwent bone marrow biopsy for rule out acute myeloid leukemia. But, on bone marrow sample, only viral cytopathic effects were seen. COVID-19 virus stimulates inflammatory cells to produces various inflammatory cytokines and as a result, viral cytopathic effects on white blood cells is seen. Conclusion: We have described how the characteristic peripheral blood findings of COVID‐19 infection can be misinterpret as acute myeloid leukemia.","PeriodicalId":8848,"journal":{"name":"Asian Pacific Journal of Cancer Biology","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85528950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-12DOI: 10.31557/apjcb.2021.6.3.201-205
M. Mohammed, N. Ahmed
Background: Evasion of apoptosis is an essential alteration for cellular genetic mutation. Survivin is a member of inhibitor of apoptosis protein (IAP) family. Under normal conditions, Survivin is expressed in embryonic and fetal tissues and markedly diminished in mature, differentiated adult tissues. Survivin was found to be re-expressed in multiple solid and hematological human malignant neoplasms. The purpose of this study was to evaluate the expression of Survivin in renal cell carcinoma (RCC), and to find statistically significant associations between Survivin and the tested Clinicopathological parameters.Methods: 39 patients with RCCs who underwent nephrectomy were included in the study. From each RCC specimen, two tissue sections were obtained; one was stained by H&E stain to determine both RCC phenotype and Fuhrman’s nuclear grades. The second tissue section was immunohistochemically stained by anti-human Survivin antibody. Results: The study revealed statistically significant associations between Survivin expression in RCC specimens and RCC histological types (p =0.002), high tumor grade (p< 0.001) and advanced tumor stage (p< 0.001). Conclusion: The study revealed that Survivin is positively correlated to poorly differentiated RCCs with high Fuhrman’s nuclear grade and advanced tumor stage.
{"title":"Significance of Immunohistochemical Expression of Survivin in Renal Cell Carcinoma","authors":"M. Mohammed, N. Ahmed","doi":"10.31557/apjcb.2021.6.3.201-205","DOIUrl":"https://doi.org/10.31557/apjcb.2021.6.3.201-205","url":null,"abstract":"Background: Evasion of apoptosis is an essential alteration for cellular genetic mutation. Survivin is a member of inhibitor of apoptosis protein (IAP) family. Under normal conditions, Survivin is expressed in embryonic and fetal tissues and markedly diminished in mature, differentiated adult tissues. Survivin was found to be re-expressed in multiple solid and hematological human malignant neoplasms. The purpose of this study was to evaluate the expression of Survivin in renal cell carcinoma (RCC), and to find statistically significant associations between Survivin and the tested Clinicopathological parameters.Methods: 39 patients with RCCs who underwent nephrectomy were included in the study. From each RCC specimen, two tissue sections were obtained; one was stained by H&E stain to determine both RCC phenotype and Fuhrman’s nuclear grades. The second tissue section was immunohistochemically stained by anti-human Survivin antibody. Results: The study revealed statistically significant associations between Survivin expression in RCC specimens and RCC histological types (p =0.002), high tumor grade (p< 0.001) and advanced tumor stage (p< 0.001). Conclusion: The study revealed that Survivin is positively correlated to poorly differentiated RCCs with high Fuhrman’s nuclear grade and advanced tumor stage. ","PeriodicalId":8848,"journal":{"name":"Asian Pacific Journal of Cancer Biology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90152250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-09DOI: 10.31557/APJCB.2021.6.3.173-179
Huzaifa Saleem, Mehwish Javed, A. Saleem, Sadia Atif, Rabia Rafi
Objective: To determine the diagnostic yield of cell blocks, prepared by the vapour fixation method, using laboratory supplies easily available at any low resource laboratory setup or outpatient department of a basic healthcare unit. Methods: Prospective descriptive study was carried out at Healthways Laboratories Rawalpindi, for 6 months from 1st January 2017 to 30th June 2017. Walk in patients referred to the lab for FNAC were selected by non probability convenient sampling. After preparing FNAC slides from first pass, the patients were briefed about the cell block material and after informed consent second dedicated passes were done to make cell blocks. Results: Out of 47 cases, there were 25 (53.2 %) breast lumps, 8 (17%) superficial collections, 7 (15.1%) lymphadenopathies, 6 (12.8%) thyroid swellings and 1 salivary gland swelling. Technique yielded moderate to high cellularity in 33 (70.2%) cases, 16 from malignant and 1 from benign breast lumps, 5 from thyroid, 3 from reactive and 3 from metastatic lymphnodes, 3 from abscesses, 1 from lipoma and 1 from salivary gland. Low cellularity 14 (29.8%) cases were from 5 benign and 3 malignant breast lumps, 2 hematomas and 1 case each from thyroid, metastatic lymphnode, abscess and lipoma. Good cellularity was achieved in 16 (84.2%) of 19 cases of malignant breast lesions, 3 (15.8%) were hypo cellular of which 1 was non-diagnostic on FNAC smear. Cell block diagnosis was comparable to FNAC in 35 of 47 (74.4%) cases. Non diagnostic cases on cell block were more, 13 versus 3 cases on FNAC. The kappa value of agreement in diagnosis was 0.64. Conclusions: Poor man’s cell block method is simplest and effective method of cell block preparation which must be advocated at basic healthcare units and low tech laboratory. Its diagnostic potential must be further explored.
{"title":"Poor Man’s Cell-block Preparation, a Novel Idea which Can be Applied to Basic Health Care Facilities in Resource-poor Settings","authors":"Huzaifa Saleem, Mehwish Javed, A. Saleem, Sadia Atif, Rabia Rafi","doi":"10.31557/APJCB.2021.6.3.173-179","DOIUrl":"https://doi.org/10.31557/APJCB.2021.6.3.173-179","url":null,"abstract":" Objective: To determine the diagnostic yield of cell blocks, prepared by the vapour fixation method, using laboratory supplies easily available at any low resource laboratory setup or outpatient department of a basic healthcare unit. Methods: Prospective descriptive study was carried out at Healthways Laboratories Rawalpindi, for 6 months from 1st January 2017 to 30th June 2017. Walk in patients referred to the lab for FNAC were selected by non probability convenient sampling. After preparing FNAC slides from first pass, the patients were briefed about the cell block material and after informed consent second dedicated passes were done to make cell blocks. Results: Out of 47 cases, there were 25 (53.2 %) breast lumps, 8 (17%) superficial collections, 7 (15.1%) lymphadenopathies, 6 (12.8%) thyroid swellings and 1 salivary gland swelling. Technique yielded moderate to high cellularity in 33 (70.2%) cases, 16 from malignant and 1 from benign breast lumps, 5 from thyroid, 3 from reactive and 3 from metastatic lymphnodes, 3 from abscesses, 1 from lipoma and 1 from salivary gland. Low cellularity 14 (29.8%) cases were from 5 benign and 3 malignant breast lumps, 2 hematomas and 1 case each from thyroid, metastatic lymphnode, abscess and lipoma. Good cellularity was achieved in 16 (84.2%) of 19 cases of malignant breast lesions, 3 (15.8%) were hypo cellular of which 1 was non-diagnostic on FNAC smear. Cell block diagnosis was comparable to FNAC in 35 of 47 (74.4%) cases. Non diagnostic cases on cell block were more, 13 versus 3 cases on FNAC. The kappa value of agreement in diagnosis was 0.64. Conclusions: Poor man’s cell block method is simplest and effective method of cell block preparation which must be advocated at basic healthcare units and low tech laboratory. Its diagnostic potential must be further explored.","PeriodicalId":8848,"journal":{"name":"Asian Pacific Journal of Cancer Biology","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85270789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-09DOI: 10.31557/APJCB.2021.6.3.195-200
T. Hoang, Thi Thu Huong Bui, T. Nguyễn
Background: Breast cancer is a common malignancy in women. DCTPP1 is a potential target for the development of antitumor drugs, and plays an important role in the process of DNA replication. Aims: To investigate the biological role of DCTPP1 gene, as well as its expression in breast cancer and its relation to patient prognosis. Materials and Methods: Breast cancer data was derived from the TCGA database. Using the UALCAN database, the expression level of DCTPP1 mRNA in breast cancer tissues was investigated. The expression of DCTPP1 in various pathological types of breast cancer was studied using the Human Protein Atlas. UALCAN was also used to investigate the relationship between DCTPP1 gene expression and breast cancer patient prognosis. Bioinformatics studied the proteins related to DCTPP1 expression and their roles in the GeneMANIA and WebGestalt databases. Results: DCTPP1 mRNA was significantly expressed in breast cancer compared to normal breast tissue (P<0.001). DCTPP1 was shown to be highly expressed in breast cancer tissues from different pathological types and stages (P<0.001). The DCTPP1 protein was expressed at a higher frequency in breast cancer than in normal breast tissue. When compared to patients with low DCTPP1 expression, patients with high DCTPP1 expression had a considerably shorter overall survival time. The 20 proteins related to DCTPP1 expression were mostly located in the nucleus and membrane, and were involved in biological regulation, stimulus response, metabolic process, and other processes, according to gene ontology analysis. It plays an important role in protein binding, ion binding, and nucleic acid binding. Conclusion: DCTPP1 is highly expressed in breast cancer, and is associated to a poor prognosis for patients with breast cancer. DCTPP1 may be a potential therapy and intervention target for breast cancer.
背景:乳腺癌是女性常见的恶性肿瘤。DCTPP1是开发抗肿瘤药物的潜在靶点，在DNA复制过程中发挥重要作用。目的:探讨DCTPP1基因在乳腺癌中的生物学作用、表达及其与患者预后的关系。材料和方法:乳腺癌数据来源于TCGA数据库。利用UALCAN数据库，研究DCTPP1 mRNA在乳腺癌组织中的表达水平。利用人蛋白图谱(Human Protein Atlas)研究DCTPP1在不同病理类型乳腺癌中的表达。UALCAN还用于研究DCTPP1基因表达与乳腺癌患者预后的关系。生物信息学研究了DCTPP1表达相关蛋白及其在GeneMANIA和WebGestalt数据库中的作用。结果:DCTPP1 mRNA在乳腺癌组织中的表达明显高于正常乳腺组织(P<0.001)。DCTPP1在不同病理类型和分期的乳腺癌组织中均有高表达(P<0.001)。DCTPP1蛋白在乳腺癌中的表达频率高于正常乳腺组织。与DCTPP1低表达的患者相比，DCTPP1高表达的患者总生存时间明显缩短。基因本体论分析显示，与DCTPP1表达相关的20个蛋白多位于细胞核和细胞膜，参与生物调控、刺激反应、代谢过程等过程。它在蛋白质结合、离子结合、核酸结合中起重要作用。结论:DCTPP1在乳腺癌中高表达，与乳腺癌患者预后不良有关。DCTPP1可能是乳腺癌潜在的治疗和干预靶点。
{"title":"Comprehensive Analysis of the Expression and Prognosis for DCTPP1 gene in Breast Cancer","authors":"T. Hoang, Thi Thu Huong Bui, T. Nguyễn","doi":"10.31557/APJCB.2021.6.3.195-200","DOIUrl":"https://doi.org/10.31557/APJCB.2021.6.3.195-200","url":null,"abstract":"Background: Breast cancer is a common malignancy in women. DCTPP1 is a potential target for the development of antitumor drugs, and plays an important role in the process of DNA replication. Aims: To investigate the biological role of DCTPP1 gene, as well as its expression in breast cancer and its relation to patient prognosis. Materials and Methods: Breast cancer data was derived from the TCGA database. Using the UALCAN database, the expression level of DCTPP1 mRNA in breast cancer tissues was investigated. The expression of DCTPP1 in various pathological types of breast cancer was studied using the Human Protein Atlas. UALCAN was also used to investigate the relationship between DCTPP1 gene expression and breast cancer patient prognosis. Bioinformatics studied the proteins related to DCTPP1 expression and their roles in the GeneMANIA and WebGestalt databases. Results: DCTPP1 mRNA was significantly expressed in breast cancer compared to normal breast tissue (P<0.001). DCTPP1 was shown to be highly expressed in breast cancer tissues from different pathological types and stages (P<0.001). The DCTPP1 protein was expressed at a higher frequency in breast cancer than in normal breast tissue. When compared to patients with low DCTPP1 expression, patients with high DCTPP1 expression had a considerably shorter overall survival time. The 20 proteins related to DCTPP1 expression were mostly located in the nucleus and membrane, and were involved in biological regulation, stimulus response, metabolic process, and other processes, according to gene ontology analysis. It plays an important role in protein binding, ion binding, and nucleic acid binding. Conclusion: DCTPP1 is highly expressed in breast cancer, and is associated to a poor prognosis for patients with breast cancer. DCTPP1 may be a potential therapy and intervention target for breast cancer.","PeriodicalId":8848,"journal":{"name":"Asian Pacific Journal of Cancer Biology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74907475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-09DOI: 10.31557/APJCB.2021.6.3.181-185
R. Golmohammadi, M. Mohajeri, A. Jarrahi, A. Moslem, A. Pejhan, A. Gohari
Objective: Contradictory reports have been published regarding the expression levels of the hormone receptors of estrogen and progesterone (ER / PR) and theirclinical importance in diagnosis of breast cancer. The aim of this study was to evaluate the relationship between pathological features of invasive and non-invasive ductal tumors by different ER / PR phenotypes. Methods: This descriptive-analytical study was performed on 74 specimens of breast cancer referred to Isfahan Hospitals for diagnosis between 2015 - 2018. After fixation of the specimens in formalin, tissue passage, cross section and H / E staining, the specimens were divided into two groups: non- invasive and Invasive ductal Carcinoma. After removing of mask, expression of different ER / PR phenotypes was performed using primary monoclonal antibody and immunohistochemically methods. Results: From 74 malignant specimens, 61 (82.4%) were in the category of invasive ductal tumors and 13 cases (17.6%) were in the category of non-invasive ductal tumors. Out of 73 patients with positive ER or PR phenotype 47 samples (63.5%) had ER + / PR +phenotypes, 6 samples had (8.1%) ER+ / PR –phenotype, 20 samples (27%) had ER- / PR + phenotype and only one sample (1.4%) had the ER- / PR- phenotype and was in the category of invasive ductal tumors. There was not detected ER- / PR- phenotype expression in non-invasive ductal tumor. Further analysis showed that there were not significant difference between ER / PR phenotype and tumor stage (p =0.36) or with tumor Grade (P=0.38), high age of menopause or post menopause (P> 0.05). Conclusion: Our data shows that expression of ER- / PR- phenotype only was detected in invasive ductal tumor. It is thought that the tumor type maybe affects the expression of different types of ER / PR hormone receptor phenotypes in breast cancer patients.
{"title":"Histopathologic Characteristics of Invasive and Non-invasive Ductal Tumors have Relationship with Different Phenotypes of ER / PR Receptors in Breast Cancer Patients","authors":"R. Golmohammadi, M. Mohajeri, A. Jarrahi, A. Moslem, A. Pejhan, A. Gohari","doi":"10.31557/APJCB.2021.6.3.181-185","DOIUrl":"https://doi.org/10.31557/APJCB.2021.6.3.181-185","url":null,"abstract":"Objective: Contradictory reports have been published regarding the expression levels of the hormone receptors of estrogen and progesterone (ER / PR) and theirclinical importance in diagnosis of breast cancer. The aim of this study was to evaluate the relationship between pathological features of invasive and non-invasive ductal tumors by different ER / PR phenotypes. Methods: This descriptive-analytical study was performed on 74 specimens of breast cancer referred to Isfahan Hospitals for diagnosis between 2015 - 2018. After fixation of the specimens in formalin, tissue passage, cross section and H / E staining, the specimens were divided into two groups: non- invasive and Invasive ductal Carcinoma. After removing of mask, expression of different ER / PR phenotypes was performed using primary monoclonal antibody and immunohistochemically methods. Results: From 74 malignant specimens, 61 (82.4%) were in the category of invasive ductal tumors and 13 cases (17.6%) were in the category of non-invasive ductal tumors. Out of 73 patients with positive ER or PR phenotype 47 samples (63.5%) had ER + / PR +phenotypes, 6 samples had (8.1%) ER+ / PR –phenotype, 20 samples (27%) had ER- / PR + phenotype and only one sample (1.4%) had the ER- / PR- phenotype and was in the category of invasive ductal tumors. There was not detected ER- / PR- phenotype expression in non-invasive ductal tumor. Further analysis showed that there were not significant difference between ER / PR phenotype and tumor stage (p =0.36) or with tumor Grade (P=0.38), high age of menopause or post menopause (P> 0.05). Conclusion: Our data shows that expression of ER- / PR- phenotype only was detected in invasive ductal tumor. It is thought that the tumor type maybe affects the expression of different types of ER / PR hormone receptor phenotypes in breast cancer patients.","PeriodicalId":8848,"journal":{"name":"Asian Pacific Journal of Cancer Biology","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87579303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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