International journal of genetics and molecular biology最新文献

{"title":"Gene Trapping: A Powerful Tool of Functional Genomics to Identify Novel Genes","authors":"K. Anjani, Vimlesh Sharma, H. Kumar","doi":"10.9735/0975-2862.10.1.325-332","DOIUrl":"https://doi.org/10.9735/0975-2862.10.1.325-332","url":null,"abstract":"","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"115 1","pages":"325"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80304142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Dus Descriptor for Casuarina Genetic Resources","authors":"K. Parthiban, N. Kanagaraj, B. Palanikumaran, N. Krishnakumar","doi":"10.9735/0975-2862.10.1.333-338","DOIUrl":"https://doi.org/10.9735/0975-2862.10.1.333-338","url":null,"abstract":"","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"1 3 1","pages":"333"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83417127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDH1 Mutation in Gliomas in Baghdad by Immunohistochemical Study","authors":"Zahraa Marwan Shaban, Salim Rasheed Al-Aubaidy, Ameer Dhahir Hameedi","doi":"10.11648/j.ijgg.20180601.11","DOIUrl":"https://doi.org/10.11648/j.ijgg.20180601.11","url":null,"abstract":"IDH1 (isocitrate dehydrogenase 1) mutation might be encounter in the low-grade glioma occurs in early stages of development and directs the progression of the tumor to a higher grade. Aim of the study was to assess the frequency of IDH1 mutation in Iraqi patients with gliomas by immunohistochemical study, to correlate its immunoreactivity with some clinicopathological parameters. The study did on formalin fixed, paraffin embedded tumor tissue from 66 patients with different grades of intracranial gliomas of both gender and all age groups in the Baghdad city were collected in this retrospective and prospective randomized study. Ten normal brain tissue samples in form of paraffin blocks took from forensic medicine unit. New technique used, which is manual tissue microarray Immunohistochemical detection of IDH1 antibodies did by Dako autostainer link 48. Positive cytoplasmic IDH1 staining was found in 38 (57.6%) of cases of glioma. In adult gliomas, secondary glioblastoma multiforme, low grade astrocytoma and oligodendroglial tumors had the greatest values of IDH1 positivity (87%, 80% and 72.72% respectively) followed by anaplastic astrocytoma (42%), then primary glioblastoma multiforme (26%). Males and females expressed the IDH1 equally. The conclusions from the work were IDH1 mutation commonly existed in adult gliomas, low-grade gliomas and secondary glioblastoma, it had no role in pediatric gliomas, and it could be a diagnostic and prognostic marker.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"42 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2018-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74585150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De novo assembly and characterization of transcriptome and microsatellite marker development for Taro (Colocasia esculenta (L.) Schott.)","authors":"Li Wang, Jianmei Yin, Peitong Zhang, Xiaoyong Han, Wenqi Guo, Chun-Hong Li","doi":"10.5897/IJGMB2017.0155","DOIUrl":"https://doi.org/10.5897/IJGMB2017.0155","url":null,"abstract":"Taro (Colocasia esculenta. (L.) Schott) is a genus of perennial plants that is widely distributed in the tropics or subtropics of Asia, Africa and America, which is the fourteenth most consumed vegetable of the world. However, molecular genetic research of Colocasia has been hindered by the insufficient genomic and transcriptome information. Here, the transcriptome of taro variety ‘Jingjiang Xiangsha’ from Jiangsu, China, was sequenced using the Illumina HiSeqTM 2000 platform in 2015. A total of 58,263,364 reads were generated, and assembly resolved into 65,878 unigenes with a N50 length of 1,357 bp. A total of 40,375 unigene sequences were successfully annotated based on searches against six public databases. Among the annotated unigenes, 14,753 were identified by gene Ontologyterms, 16,643 were classified to Clusters of Orthologous Groups categories, and 25,401 were mapped to 127 pathways in the Kyoto Encyclopedia of genes and genomes database. Also, 11,363 potential microsatellite loci were identified in 5,671 unigenes, and 150 primer pairs were randomly selected and amplified in 18 accessions of C. esculenta. A total of 100 primer pairs showed polymorphisms in repeat length. The number of alleles per locus ranged from 2 to 8. Across the 100 microsatellite loci, the polymorphism information content values ranged from 0.042 to 0.778. The transcriptomic data and microsatellite markers will play important roles in future functional gene analyses and genetic map construction of taro. \u0000 \u0000   \u0000 \u0000 Key words: Colocasia, genetic diversity, microsatellite markers, transcriptome.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"40 1","pages":"26-36"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81365354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Broad sense heritability and genetic advance for grain yield and yield components of chickpea (Cicer arietinum L.) genotypes in western Ethiopia","authors":"Biruk Alemu, K. Tesfaye, T. Haileselassie, D. Lule","doi":"10.5897/IJGMB2017.0158","DOIUrl":"https://doi.org/10.5897/IJGMB2017.0158","url":null,"abstract":"Genetic variability is a prerequisite to crop improvement. The objective of the present study was to assess and quantify the genetic variability, estimate heritability and genetic advance for yield and yield contributing characters of released and pipeline chickpea varieties based on agro- morphological traits. A total of 16 chickpea materials (8 improved varieties, 7 advanced lines, and one local check) were planted in Randomized Complete Block Design (RCBD) with three replications at Shambu, Hawa Galan, Mata, Alaku Belle, and Badesso, in Western Ethiopia. Important agronomic data were collected and subjected to analysis using statistical analysis software (SAS). The combined analysis of variance (ANOVA) indicated highly significant differences (P≤0.01) among chickpea genotypes for grain yield and other agronomic traits except for number of seed per pod and branch per plant, indicating the existence of ample genetic variability among present chickpea genotypes. Genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) for the character such as grain filling duration, days to flowering and hundred seed weight, showed very small difference demonstrating that the observed variations for these traits were mostly due to genetic factors with little impact of environment. The higher heritability coupled with higher genetic advance was noted for 100 seed weight, number of pods per plant, number of seed per pod and grain yield signifying the ease of phenotype-based selection for the improvement of those traits. \u0000 \u0000   \u0000 \u0000 Key words: Chickpea (Cicer airetinum L.), genetic variability, phenotypic coefficient of variation (PCV), genotypic coefficient of variation (GCV), selection.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"35 1","pages":"21-25"},"PeriodicalIF":0.0,"publicationDate":"2017-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88059019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Throughput Sequencing Reveals miRNAs Affecting Follicle Development in Chicken","authors":"Quan Zhang, L. Shanshan, B. Du","doi":"10.11648/J.IJGG.20170506.12","DOIUrl":"https://doi.org/10.11648/J.IJGG.20170506.12","url":null,"abstract":"As the derivative of chicken skin, hair follicle is capable of self-renew. Its proliferation and differentiation result in hair formation. MicroRNAs (miRNAs) can effectively regulate gene expression at the post-transcriptional level and play a critical role in tissue growth, development. In this study, we used next generation sequencing technology sequenced miRNAs of the hair follicle derived from the 13 day-old chicken (Gallus gallus) embryos in which from Kirin chicken and Huaixiang chicken that feathers having morphogenesis with significantly different curling. A population of conserved miRNAs was identified. These conserved miRNAs were derived from 638 homologous hairpin precursors across 5 animal species. We identified a total of 645 miRNAs in the chicken embryos. Among them, 11 differentially expressed miRNAs were identified (> ±2 Fold, p value <0.05) by comparing Kirin chicken and Huaixiang chicken. Several gene ontology (GO) biology processes and the WNT, BMP and TGF-β signaling pathways were found to be differentially expressed miRNAs as part of hair follicle development process. The miR-1623 has an effect on WNT4 and involved in hair follicle cell development. This study has identified miRNAs that associated with the chick embryonic hair follicle development and identified some target miRNAs for further research into their role played in feather growth.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"236 1","pages":"76"},"PeriodicalIF":0.0,"publicationDate":"2017-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81123301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Variability and Association Among Agronomic Characters in Selected Field Pea ( Pisumsativum L. ) Genotypes at Bale Zone, Sinana Research Center, Oromia Region, Ethiopia","authors":"Benti Ofga, Yohannis Petros","doi":"10.11648/J.IJGG.20170506.11","DOIUrl":"https://doi.org/10.11648/J.IJGG.20170506.11","url":null,"abstract":"Field pea ( Pisumsativum L. ) is an annual herbaceous legume belonging to the family Fabaceae that adapted to cool moist climate with moderate temperature. The species P. sativumis dominant in Ethiopia even though wild and primitive forms are also known to exist in the high elevation of the country. No sufficient work has been done for understanding and describing the nature and extent of genetic variability on Field Pea. Thus use of genetic variability is suggested to alleviate the shortage of released varieties in Field pea. The studies of association characters solve the low yield of field pea in Ethiopia. Therefore, the present study was conducted to study genetic variability and association among agronomic characters of field pea ( Pisumsativum L. ) genotypes. The field experiment was conducted using 29 varieties and 7 accessions at Sinana Agricultural Research Centre during 2013/14 main growing season. Treatments were arranged in Simple Lattice design with two replications. The results obtained revealed that the mean squares of the genotypes were highly significant for most of the characters (p < 0.05) except for days to emergence, days to flowering, pods per plant, above ground biomass and harvest index implying that wide range of variability has been obtained for the traits studied. The genotypic coefficient of variation ranged from 2.91% for plant height to 37.9% for above ground biomass, while phenotypic coefficient of variation ranged from 6.28% for days to maturity to 62.6% for above ground biomass. The estimated broad sense heritability ranged from 6.9% for yield per plant to 93.0.0% for pod length. Correlation between different traits and seed yield indicated that there was marked positive and highly significant association of seed yield with days to emergence (rg=0.296**), days to maturity (rg=0.184**), seeds per pod (rg=0.325**) and harvest index (rg=0.341**) indicating the yield improvement perhaps be contributed to changes in these traits. The path analysis at phenotypic level revealed that maximum positive direct effect was exerted by 100 seed weight (1.754) followed by pod length (2.211) and days to emergence (2.056). These traits best contributing to yield variation that emphasis by breeders for future yield improvement of the crop. It can be conclude that Field pea yield can be successfully improved by studying genetic variability and its agronomic characters.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"121 1","pages":"63"},"PeriodicalIF":0.0,"publicationDate":"2017-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86162123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inter-species Transferability of Microsatellite Markers Derived from Wild Relatives to Cultivated Species of Finger Millet","authors":"Nagabhushana Kotrappa, S. Hittalmani, Krishna Venkatarangaiah","doi":"10.11648/j.ijgg.20170504.12","DOIUrl":"https://doi.org/10.11648/j.ijgg.20170504.12","url":null,"abstract":"The accessibility to public sequence information has paved the way for development of new genomic resources and its cross transferability among closely related genera. In the present study, the nucleotide and EST sequences derived from nine different species of Eleusine were utilized for identifying microsatellite markers and their transferability in E. coracana. The frequency and distribution of repeat motifs of Di, tri, tetra and penta-nucleotide repeats were compared across species. The nucleotide/EST sequences, classified based on their function, were majorly involved in abiotic stress followed by carbohydrate biosynthesis in all the species. Of 2133 primers designed, tri-nucleotide repeats were more abundant (1043) followed by repeats in compound format (963). A highest number of 1660 primer pairs were identified in E. coracana subsp. coracana and nearly 50% of which contained compound repeats, majorly comprising of di-nucleotides. The frequency of microsatellite repeats and the number of primers designed per sequence were maximum in E. kigeziensis (138%) followed by E. floccifolia (126%) while the same was minimum in E. coracana subsp. africana (53%). While the transferability of microsatellites derived from other Eleusine species to cultivated coracana species ranged from 50 to 100%, the primers derived from cultivated species were more informative than from that of wild relatives.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"1 1","pages":"42"},"PeriodicalIF":0.0,"publicationDate":"2017-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76700382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetics of Flower Color in Periwinkle Catharanthus roseus (L) G. Don","authors":"A. H. Abdelmageed, Mohamed Abdelrahman","doi":"10.11648/J.IJGG.20170504.11","DOIUrl":"https://doi.org/10.11648/J.IJGG.20170504.11","url":null,"abstract":"Genetics of flower Color in winka Catharanthus roseus (L) G.Don were investigate by inheritance two types (strains) of plants with different flowers color were used in this study, violet (V) and White (W) color as parents, to determine the number of genes involved. This study was conducted at the Department of Chemistry & Biology at the Faculty of Education, University of Kassala, kassala State, Sudan, during: the autumn, seasons for two years 2010- 2012. First the two parents were covered to ensure self pollination. Reciprocal cross has been carried out between the two inbred parents. The study showed that a single pair of genes is probably involved in flower colour and that gene for violet color is incompletely dominant over that for white color. The reciprocal crosses gave the same results indicating no role of cytoplasmic genes in the inheritance of these colors.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"274 1","pages":"36"},"PeriodicalIF":0.0,"publicationDate":"2017-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76128679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Individuals in a DNA Mixture Using SNP Markers","authors":"Abdulwasiu Ibrahim","doi":"10.11648/J.IJGG.20170503.11","DOIUrl":"https://doi.org/10.11648/J.IJGG.20170503.11","url":null,"abstract":"This article focuses mainly on DNA mixture from two contributors, a victim and an unknown culprit. There are two areas I believe will be of interest to forensic scientists, police and a Jury. These areas are identification of an individual in a DNA mixture and familial DNA database searching of a culprit through a relative. In this article, I looked at identification of individuals in a mixture using Single Nucleotide Polymorphisms (SNPs) markers. SNPs are starting to be used for forensic identification; I employed them as they produce incredible results for identification in a two-person mixture. The conservative method I employed here is the random man not excluded probability – P (RMNE) approach, an inclusion probability method generally considered as a frequentist approach. It was found that an optimum allele frequency of 0.2 is required to produce almost certain identification with much distortion in identifying an individual even when inbreeding is up to 50% in a population. Another interesting thing is that relatives of a suspect whom are actual contributors to the DNA mixture can also be identified. In a case where there are relatives in the mixture it was found that twice the number of SNP panels is required to identify an individual than in a case where no relative is involved. And lastly, typing more SNP panels helps to improve identification and therefore produce forensically useful results.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"12 1","pages":"27"},"PeriodicalIF":0.0,"publicationDate":"2017-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74006568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}