SAMADI SAMADI, SITTI WAJIZAH, SAID MIRZA PRATAMA, ANURAGA JAYANEGARA
Abstract. Samadi, Wajizah S, Pratama SM, Jayanegara A. 2023. Evaluation of nutritive values of various non-conventional protein sources as potential feed ingredients for ruminants. Biodiversitas 24: 4069-4078. The purpose of this study was to evaluate the nutritive values of various non-conventional protein sources as potential ingredients for ruminants, including chemical composition, in vitro digestibility, rumen fermentation, and methane emissions. Seven various non-conventional protein sources; grasshopper meal (GM), earthworm meal (EWM), fish by-product meal (FbPM), centipede meal (CM), snail meal (SM), ant eggs meal (AEM), mealworm meal (MWM) and one conventional protein source; soybean meal (SBM) were used in this study. All samples were dried at the temperature of 60oC for 24h and ground by the use of a hammer mill to pass a 1 mm sieve. The ground samples were utilized for further analysis, including chemical composition, in vitro digestibility, rumen fermentation, and methane emissions. Incubation was conducted with three replications in the water bath and temperature was maintained at 39ºC for 48h. All data were statistically analyzed using SPSS version 16 and the difference between treatments was stated (P<0.05). The results of the study indicated that all the samples contained various CP. The NDICP and ADICP in samples were low. All samples had high IVDMD and IVOMD, the highest in FbPM (P<0.01). Methane emission of the samples was lower than SBM (P<0.01).
摘要张建军，张建军，张建军，等。各种非传统蛋白质来源作为反刍动物潜在饲料原料的营养价值评价。生物多样性，24:4069-4078。本研究的目的是评估各种非传统蛋白质来源作为反刍动物潜在原料的营养价值，包括化学成分、体外消化率、瘤胃发酵和甲烷排放。7种不同的非常规蛋白质来源;蚱蜢粉(GM)、蚯蚓粉(EWM)、鱼副产物粉(FbPM)、蜈蚣粉(CM)、蜗牛粉(SM)、蚁卵粉(AEM)、粉虫粉(MWM)和一种常规蛋白质源;本研究以豆粕(SBM)为原料。所有样品在60℃下干燥24h，用锤式磨粉机研磨，通过1 mm筛。利用地面样品进行进一步分析，包括化学成分、体外消化率、瘤胃发酵和甲烷排放。在水浴中孵育3次，温度保持在39℃，孵育48小时。所有数据均采用SPSS version 16进行统计学分析，并说明处理间差异(P<0.05)。研究结果表明，所有样品均含有不同的CP，样品的NDICP和ADICP较低。所有样品均具有较高的IVDMD和IVOMD，其中FbPM最高(P<0.01)。样品的甲烷排放量低于SBM (P<0.01)。
{"title":"Evaluation of nutritive values of various non-conventional protein sources as potential feed ingredients for ruminants","authors":"SAMADI SAMADI, SITTI WAJIZAH, SAID MIRZA PRATAMA, ANURAGA JAYANEGARA","doi":"10.13057/biodiv/d240745","DOIUrl":"https://doi.org/10.13057/biodiv/d240745","url":null,"abstract":"Abstract. Samadi, Wajizah S, Pratama SM, Jayanegara A. 2023. Evaluation of nutritive values of various non-conventional protein sources as potential feed ingredients for ruminants. Biodiversitas 24: 4069-4078. The purpose of this study was to evaluate the nutritive values of various non-conventional protein sources as potential ingredients for ruminants, including chemical composition, in vitro digestibility, rumen fermentation, and methane emissions. Seven various non-conventional protein sources; grasshopper meal (GM), earthworm meal (EWM), fish by-product meal (FbPM), centipede meal (CM), snail meal (SM), ant eggs meal (AEM), mealworm meal (MWM) and one conventional protein source; soybean meal (SBM) were used in this study. All samples were dried at the temperature of 60oC for 24h and ground by the use of a hammer mill to pass a 1 mm sieve. The ground samples were utilized for further analysis, including chemical composition, in vitro digestibility, rumen fermentation, and methane emissions. Incubation was conducted with three replications in the water bath and temperature was maintained at 39ºC for 48h. All data were statistically analyzed using SPSS version 16 and the difference between treatments was stated (P<0.05). The results of the study indicated that all the samples contained various CP. The NDICP and ADICP in samples were low. All samples had high IVDMD and IVOMD, the highest in FbPM (P<0.01). Methane emission of the samples was lower than SBM (P<0.01).","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135065010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NUR FATMA, METUSALACH METUSALACH, NURPUDJI ASTUTI TASLIM, MALA NURILMALA
Abstract. Fatma N, Metusalach, Taslim NA, Nurilmala M. 2023. SDS-PAGE protein profile of albumin extracted by steaming from four marine and three brackish-water fishes. Biodiversitas 24: 4027-4033. Snakehead fish is a type of fish that has been known as the best source of fish albumin. However, the increasing demand for and exploitation of snakehead fish albumin is not accompanied by the availability of sustainable snakehead fish stocks. Snakehead fish stocks in nature continue to experience depletion and cultivation efforts that have been carried out so far have not yielded satisfactory results. For this reason, it is necessary to find alternative sources of fish albumin. The purpose of this study was to determine the molecular weight of the albumin proteins extracted by water bath steaming from four marine fishes (Indian scad Decapterus russelli, short-bodied mackerel Rastrelliger brachysoma, goldband fusilier Pterocaesio chrysozona, Japanese threadfin bream Nemipterus japonicus) and three brackish-water fishes (white-lipped eel catfish Paraplotosus albilabris, barramundi Lates calcarifer, milkfish Chanos chanos). The method used was Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) electrophoresis. Each sample was steamed in a water bath at 50°C for 30 minutes to obtain the crude extract's optimal yield and albumin content. The molecular weights of each protein band were analyzed using the Photocapt application, visualized and tabulated, and analyzed descriptively. Indian scad had six protein bands with three major (48 kD, 37 kD, 32 kD) and three minor bands (66 kD, 25 kD, 15 kD). Shortbodied mackerel had one major protein band (26 kD). Goldband fusilier had one major (33 kD) and four minor bands (50 kD, 69 kD, 57 kD, 26 kD). Japanese threadfin bream had one major (62 kD) and three minor bands (171 kD, 72 kD, 26 kD). White-lipped eel catfish had two major (41 kD, 34 kD and two minor bands (70 kD, 26 kD). Barramundi had one major (26 kD) and two minor bands (171 kD, 87 kD). Milkfish had one major (41 kD) and four minor bands (333 kD, 135 kD, 31 kD, 25 kD). It is hoped that this research will provide information on which fish have the potential as a source of fish albumin in terms of protein composition which resembles snakehead fish so that the properties provided are as good as albumin from snakehead fish.
{"title":"SDS-PAGE protein profile of albumin extracted by steaming from four marine and three brackish-water fishes","authors":"NUR FATMA, METUSALACH METUSALACH, NURPUDJI ASTUTI TASLIM, MALA NURILMALA","doi":"10.13057/biodiv/d240740","DOIUrl":"https://doi.org/10.13057/biodiv/d240740","url":null,"abstract":"Abstract. Fatma N, Metusalach, Taslim NA, Nurilmala M. 2023. SDS-PAGE protein profile of albumin extracted by steaming from four marine and three brackish-water fishes. Biodiversitas 24: 4027-4033. Snakehead fish is a type of fish that has been known as the best source of fish albumin. However, the increasing demand for and exploitation of snakehead fish albumin is not accompanied by the availability of sustainable snakehead fish stocks. Snakehead fish stocks in nature continue to experience depletion and cultivation efforts that have been carried out so far have not yielded satisfactory results. For this reason, it is necessary to find alternative sources of fish albumin. The purpose of this study was to determine the molecular weight of the albumin proteins extracted by water bath steaming from four marine fishes (Indian scad Decapterus russelli, short-bodied mackerel Rastrelliger brachysoma, goldband fusilier Pterocaesio chrysozona, Japanese threadfin bream Nemipterus japonicus) and three brackish-water fishes (white-lipped eel catfish Paraplotosus albilabris, barramundi Lates calcarifer, milkfish Chanos chanos). The method used was Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) electrophoresis. Each sample was steamed in a water bath at 50°C for 30 minutes to obtain the crude extract's optimal yield and albumin content. The molecular weights of each protein band were analyzed using the Photocapt application, visualized and tabulated, and analyzed descriptively. Indian scad had six protein bands with three major (48 kD, 37 kD, 32 kD) and three minor bands (66 kD, 25 kD, 15 kD). Shortbodied mackerel had one major protein band (26 kD). Goldband fusilier had one major (33 kD) and four minor bands (50 kD, 69 kD, 57 kD, 26 kD). Japanese threadfin bream had one major (62 kD) and three minor bands (171 kD, 72 kD, 26 kD). White-lipped eel catfish had two major (41 kD, 34 kD and two minor bands (70 kD, 26 kD). Barramundi had one major (26 kD) and two minor bands (171 kD, 87 kD). Milkfish had one major (41 kD) and four minor bands (333 kD, 135 kD, 31 kD, 25 kD). It is hoped that this research will provide information on which fish have the potential as a source of fish albumin in terms of protein composition which resembles snakehead fish so that the properties provided are as good as albumin from snakehead fish.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":"110 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135064795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract. Suharti S, Novrariani N, Wiryawan KG. 2023. Short Communication: Morphological, biochemical, and molecular identification of cellulolytic bacteria isolated from feces of endemic tropical herbivores. Biodiversitas 24: 4046-4051. Indonesia has endemic herbivores that consume lignocellulose feedstuffs including grass, tree foliage, rice straw, and legume, indicating the presence of cellulolytic bacteria in their gastrointestinal tracts. Therefore, the aim of this study was to isolate and identify cellulolytic bacteria from the feces of tropical endemic herbivores, including anoa (Bubalus depressicornis), banteng (Bos javanicus), muntjak (Muntiacus muntjak), and timor deer (Rusa timorensis). Bacteria were isolated using the serial dilution technique and screened on Carboxy Methyl Cellulose (CMC) media. The selected isolates were identified based on their morphological and biochemical characteristics, cellulase enzyme activity, and molecular identification of 16S rDNA. The result showed that a total of five bacterial isolates were isolated from feces of anoa, banteng, muntjak, and timor deer. In addition, isolates exhibited characteristics of facultative anaerobes with gram-positive coccus, fermenting glucose, fructose, sucrose, starch, and cellulose. Based on cellulolytic index, isolates from anoa and banteng feces showed high cellulolytic activity with an index of about 1.2, indicating their potential as cellulose-degrading bacteria. Molecular identification and phylogeny analysis of cellulolytic bacteria isolates from anoa and banteng feces showed 100% similarities with Enterococcus faecium. Therefore, bacteria from feces of tropical endemic herbivores, especially anoa and banteng, possess cellulolytic activity and have potential as cellulolytic probiotic for ruminants that feed on forage-based diet. This is the first study to document the cellulolytic activity of anoa, and banteng feces.
{"title":"Short Communication: Morphological, biochemical, and molecular identification of cellulolytic bacteria isolated from feces of endemic tropical herbivores","authors":"SRI SUHARTI, NUR NOVRARIANI, KOMANG G. WIRYAWAN","doi":"10.13057/biodiv/d240742","DOIUrl":"https://doi.org/10.13057/biodiv/d240742","url":null,"abstract":"Abstract. Suharti S, Novrariani N, Wiryawan KG. 2023. Short Communication: Morphological, biochemical, and molecular identification of cellulolytic bacteria isolated from feces of endemic tropical herbivores. Biodiversitas 24: 4046-4051. Indonesia has endemic herbivores that consume lignocellulose feedstuffs including grass, tree foliage, rice straw, and legume, indicating the presence of cellulolytic bacteria in their gastrointestinal tracts. Therefore, the aim of this study was to isolate and identify cellulolytic bacteria from the feces of tropical endemic herbivores, including anoa (Bubalus depressicornis), banteng (Bos javanicus), muntjak (Muntiacus muntjak), and timor deer (Rusa timorensis). Bacteria were isolated using the serial dilution technique and screened on Carboxy Methyl Cellulose (CMC) media. The selected isolates were identified based on their morphological and biochemical characteristics, cellulase enzyme activity, and molecular identification of 16S rDNA. The result showed that a total of five bacterial isolates were isolated from feces of anoa, banteng, muntjak, and timor deer. In addition, isolates exhibited characteristics of facultative anaerobes with gram-positive coccus, fermenting glucose, fructose, sucrose, starch, and cellulose. Based on cellulolytic index, isolates from anoa and banteng feces showed high cellulolytic activity with an index of about 1.2, indicating their potential as cellulose-degrading bacteria. Molecular identification and phylogeny analysis of cellulolytic bacteria isolates from anoa and banteng feces showed 100% similarities with Enterococcus faecium. Therefore, bacteria from feces of tropical endemic herbivores, especially anoa and banteng, possess cellulolytic activity and have potential as cellulolytic probiotic for ruminants that feed on forage-based diet. This is the first study to document the cellulolytic activity of anoa, and banteng feces.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135065012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LISNAWITA LISNAWITA, AHMAD RAFIQI TANTAWI, MARYANI CYCCU TOBING, ARTHA JUNITA HUTAHAYAN, WISMAROH SANNIWATI SARAGIH, DEWI SARTIAMI
Abstract. Lisnawita, Tantawi AR, Tobing MC, Hutahayan AJ, Saragih WS, Sartiami D. 2023. The vector of Pineapple Mealybug Wilt-associated Virus (PMWaV) in Sipahutar pineapples in North Tapanuli, Indonesia. Biodiversitas 24: 4052-4059. The Pineapple Mealybug Wilt-associated Virus (PMWaV) disease is one of the most important diseases in pineapples. It Is widely spread in almost all pineapple plantations, including those in North Tapanuli District, North Sumatra Province, Indonesia. Currently, there is still little information on the PMWaV disease and the species of mealybug found in Sipahutar pineapples in the North Tapanuli District. This research aims to identify mealybugs as the vectors that carry the virus that causes PMWaV disease in Sipahutar pineapples. Mealybug samples were taken from 12 villages in four sub-districts in the center of Sipahutar pineapples plantations in the North Tapanuli District. The four sub-districts were Sipahutar Sub-district from Siabal Abal IV Village, Sabungan Nihuta I Village, and Sabungan Nihuta II Village; Siborongborong Sub-district from Bahal Batu I Village, Bahal Batu II Village, and Lobu Siregar Village; Tarutung Sub-district from Parbaju Tonga Village, Jambur Nauli Village, and Sihujur Village; Pangaribuan Sub-district from Pansurnatolu Village, Sigotom Village, and Rahut Bosi Village. Samples were identified morphologically and molecularly. The results of the identification using both methods showed that the mealybugs found on Sipahutar pineapples with PMWaV symptoms from the 12 villages were Dysmicoccus brevipes species.
摘要李建军，张建军，张建军，张建军，张建军。印度尼西亚北塔帕努里Sipahutar凤梨中菠萝粉蚧萎蔫相关病毒(PMWaV)的媒介研究。生物多样性24:4052-4059。菠萝粉蚧萎蔫相关病毒(PMWaV)病是菠萝的主要病害之一。它在几乎所有菠萝种植园中广泛传播，包括在印度尼西亚北苏门答腊省北塔帕努里区。目前，关于在北塔帕努里区Sipahutar菠萝中发现的PMWaV疾病和粉蚧种类的信息仍然很少。这项研究的目的是确定粉蚧作为媒介携带病毒，导致PMWaV病在Sipahutar菠萝。粉蚧样本取自北塔帕努里区Sipahutar菠萝种植园中心4个街道的12个村庄。四个街道分别是Siabal Abal IV村的Sipahutar街道、Sabungan Nihuta I村和Sabungan Nihuta II村;从Bahal Batu I村、Bahal Batu II村和Lobu Siregar村开始的Siborongborong街道;从Parbaju Tonga村、Jambur Nauli村和sihujurr村到Tarutung街道;Pangaribuan街道由Pansurnatolu村、Sigotom村和Rahut Bosi村组成。对样品进行了形态学和分子鉴定。两种方法的鉴定结果均表明，12个村有PMWaV症状的Sipahutar菠萝上发现的粉蚧均为短丝微球菌。
{"title":"The vector of Pineapple Mealybug Wilt-associated Virus (PMWaV) in Sipahutar pineapples in North Tapanuli, Indonesia","authors":"LISNAWITA LISNAWITA, AHMAD RAFIQI TANTAWI, MARYANI CYCCU TOBING, ARTHA JUNITA HUTAHAYAN, WISMAROH SANNIWATI SARAGIH, DEWI SARTIAMI","doi":"10.13057/biodiv/d240743","DOIUrl":"https://doi.org/10.13057/biodiv/d240743","url":null,"abstract":"Abstract. Lisnawita, Tantawi AR, Tobing MC, Hutahayan AJ, Saragih WS, Sartiami D. 2023. The vector of Pineapple Mealybug Wilt-associated Virus (PMWaV) in Sipahutar pineapples in North Tapanuli, Indonesia. Biodiversitas 24: 4052-4059. The Pineapple Mealybug Wilt-associated Virus (PMWaV) disease is one of the most important diseases in pineapples. It Is widely spread in almost all pineapple plantations, including those in North Tapanuli District, North Sumatra Province, Indonesia. Currently, there is still little information on the PMWaV disease and the species of mealybug found in Sipahutar pineapples in the North Tapanuli District. This research aims to identify mealybugs as the vectors that carry the virus that causes PMWaV disease in Sipahutar pineapples. Mealybug samples were taken from 12 villages in four sub-districts in the center of Sipahutar pineapples plantations in the North Tapanuli District. The four sub-districts were Sipahutar Sub-district from Siabal Abal IV Village, Sabungan Nihuta I Village, and Sabungan Nihuta II Village; Siborongborong Sub-district from Bahal Batu I Village, Bahal Batu II Village, and Lobu Siregar Village; Tarutung Sub-district from Parbaju Tonga Village, Jambur Nauli Village, and Sihujur Village; Pangaribuan Sub-district from Pansurnatolu Village, Sigotom Village, and Rahut Bosi Village. Samples were identified morphologically and molecularly. The results of the identification using both methods showed that the mealybugs found on Sipahutar pineapples with PMWaV symptoms from the 12 villages were Dysmicoccus brevipes species.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":"7-8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135065005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MUHAMMAD HANAFIAH, FARIDA ATHAILLAH, T. ZAHRIAL HELMI, AMALIA SUTRIANA
Abstract. Hanafiah M, Athaillah F, Helmi TZ, Sutriana A. 2023. Morphology of Setaria spp. (Setariidae; Nematoda) in Aceh cattle, Indonesia. Biodiversitas 24: 4151-4160. Setaria spp. filaria usually inhabit the peritoneal cavity and its microfilaria can be found in cattle blood. Adult worms are considered non-pathogenic but may cause mild fibrinous peritonitis. However, microfilaria can cause serious conditions. The worms migrate randomly to the central nervous system in a non-natural host such as sheep, horses, and goats, causing serious neuropathological symptoms which are known as epizootic cerebrospinal setariosis or nematodiasis cerebrospinal. The objective of this research was to determine the prevalence of Setaria spp. microfilaria in the blood of infected Aceh cattle, identify the worm species in the peritoneal cavity, and determine the morphological difference of Setaria spp. through light microscope (LM) and Scanning Electron Microscope (SEM). This research was performed on 150 Aceh cattle slaughtered in Banda Aceh slaughterhouse. The hematocrit microcapillary method and modified Knott’s Technique were used to determine the presence of Setaria spp. microfilaria in blood, while LM and SEM were used to identify Setaria spp. adult worm. The results showed that Setaria spp. was detected in Aceh cattle blood based on modified Knott’s Technique and hematocrit microcapillary method with a similar prevalence of 13.3%. The worm identification revealed the presence of Setaria spp. in the peritoneal cavity of Aceh cattle. The morphological differences between Setaria digitata and Setaria labiatopapillosa were successfully discovered through LM and SEM studies.
{"title":"Morphology of Setaria spp. (Setariidae; Nematoda) in Aceh cattle, Indonesia","authors":"MUHAMMAD HANAFIAH, FARIDA ATHAILLAH, T. ZAHRIAL HELMI, AMALIA SUTRIANA","doi":"10.13057/biodiv/d240754","DOIUrl":"https://doi.org/10.13057/biodiv/d240754","url":null,"abstract":"Abstract. Hanafiah M, Athaillah F, Helmi TZ, Sutriana A. 2023. Morphology of Setaria spp. (Setariidae; Nematoda) in Aceh cattle, Indonesia. Biodiversitas 24: 4151-4160. Setaria spp. filaria usually inhabit the peritoneal cavity and its microfilaria can be found in cattle blood. Adult worms are considered non-pathogenic but may cause mild fibrinous peritonitis. However, microfilaria can cause serious conditions. The worms migrate randomly to the central nervous system in a non-natural host such as sheep, horses, and goats, causing serious neuropathological symptoms which are known as epizootic cerebrospinal setariosis or nematodiasis cerebrospinal. The objective of this research was to determine the prevalence of Setaria spp. microfilaria in the blood of infected Aceh cattle, identify the worm species in the peritoneal cavity, and determine the morphological difference of Setaria spp. through light microscope (LM) and Scanning Electron Microscope (SEM). This research was performed on 150 Aceh cattle slaughtered in Banda Aceh slaughterhouse. The hematocrit microcapillary method and modified Knott’s Technique were used to determine the presence of Setaria spp. microfilaria in blood, while LM and SEM were used to identify Setaria spp. adult worm. The results showed that Setaria spp. was detected in Aceh cattle blood based on modified Knott’s Technique and hematocrit microcapillary method with a similar prevalence of 13.3%. The worm identification revealed the presence of Setaria spp. in the peritoneal cavity of Aceh cattle. The morphological differences between Setaria digitata and Setaria labiatopapillosa were successfully discovered through LM and SEM studies.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135065015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
WAHYU ISRONI, ZULKISAM PRAMUDIA, ANJAS SASANA BAHRI, MEGA ASRI RISQIANA, NURUL MAULIDA, TIA DWI IRAWANDANI
Abstract. Isroni W, Pramudia Z, Bahri AS, Risqiana MA, Maulida N, Irawandani TD. 2023. Comparative study of the application fish apartments in Situbondo and Probolinggo, East Java, Indonesia. Biodiversitas 24: 4034-4045. Fisheries resources which are common property in their utilization are vulnerable to overfishing conditions due to exploratory actions. In addition, the use of destructive fishing gear and habitat degradation has a great impact on fisheries productivity which continues to decrease. Fish apartments as alternative artificial habitat have been successfully applied in Probolinggo, especially for coral organisms. This study compares the application of fish apartments in two different locations, Karang Katon Probolinggo and Karang Mayit Beach Situbondo. Through descriptive and valuative approaches, the survey results showed that in Karang Katon there were 5 species, the highest composition level is Acanthurus achilles (51.56%).Karang Mayit Beach there were 3 species, the highest composition level is Chaetodon octofasciatus (60.56%). The highest abundance is Acanthurus achilles (34.4 ind/m2) in Karang Katon and Chaetodon octofasciatus (42.2 ind/m2) in Karang Mayit Beach. The diversity index in Karang Katon-1.280, while in Karang Mayit Beach-0.853. The dominance index in Karang Katon 0.343, while in Karang Mayit Beach 0.496. Based on this research, the analysis showed positive results for both different locations, indicating it can be used as new habitats for sustainability and increased productivity of fisheries resources.
{"title":"Comparative study of the application fish apartments in Situbondo and Probolinggo, East Java, Indonesia","authors":"WAHYU ISRONI, ZULKISAM PRAMUDIA, ANJAS SASANA BAHRI, MEGA ASRI RISQIANA, NURUL MAULIDA, TIA DWI IRAWANDANI","doi":"10.13057/biodiv/d240741","DOIUrl":"https://doi.org/10.13057/biodiv/d240741","url":null,"abstract":"Abstract. Isroni W, Pramudia Z, Bahri AS, Risqiana MA, Maulida N, Irawandani TD. 2023. Comparative study of the application fish apartments in Situbondo and Probolinggo, East Java, Indonesia. Biodiversitas 24: 4034-4045. Fisheries resources which are common property in their utilization are vulnerable to overfishing conditions due to exploratory actions. In addition, the use of destructive fishing gear and habitat degradation has a great impact on fisheries productivity which continues to decrease. Fish apartments as alternative artificial habitat have been successfully applied in Probolinggo, especially for coral organisms. This study compares the application of fish apartments in two different locations, Karang Katon Probolinggo and Karang Mayit Beach Situbondo. Through descriptive and valuative approaches, the survey results showed that in Karang Katon there were 5 species, the highest composition level is Acanthurus achilles (51.56%).Karang Mayit Beach there were 3 species, the highest composition level is Chaetodon octofasciatus (60.56%). The highest abundance is Acanthurus achilles (34.4 ind/m2) in Karang Katon and Chaetodon octofasciatus (42.2 ind/m2) in Karang Mayit Beach. The diversity index in Karang Katon-1.280, while in Karang Mayit Beach-0.853. The dominance index in Karang Katon 0.343, while in Karang Mayit Beach 0.496. Based on this research, the analysis showed positive results for both different locations, indicating it can be used as new habitats for sustainability and increased productivity of fisheries resources.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":"202 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135064785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract. Nitiworakarn S, Phae-Ngam W, Vanijajiva O. 2023. Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers. Biodiversitas 24: 4060-4068. Musa cultivars are mostly obtained from crossing between wild diploid subspecies of M. acuminata (A genome) and M. balbisiana (B genome), and they reveal numerous levels of ploidy and genomic constitution. The present study, for the first time, investigated the genetic diversity of 90 Musa cultivars from 17 localities of Chai Badan district in Lopburi Province, Thailand, using start codon targeted (SCoT) markers. The SCoT technique has shown very high repeatability for characterizing Musa cultivars. The result indicated that DNA fingerprints from 30 SCoT primers generated 244 amplicons, among which 238 bands (97.54%) were polymorphic, with a mean of 7.93 polymorphic bands per primer. The average polymorphism information content (PIC) was high, ranging from 0.345 to 0.483, with an average of 0.432. Genetic diversity and its partitioning parameters were calculated and demonstrated that present Musa cultivars maintain relatively high genetic diversity. The unweighted pair-group method with an arithmetic cluster analysis, principal coordinate analysis and STRUCTURE analysis results were the same and showed the clear division of the genotypes into two distinct clusters, which were cluster-I consisted of only the AA genomes and cluster-II comprised of ABB and BB genomes. In conclusion, SCoT markers obtained here showed their manipulation in genetic diversity and the relationship of cultivated Musa, which would be practical for genetic preservation and continuing breeding programs in Thailand and other regions.
{"title":"Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers","authors":"SARUDA NITIWORAKARN, WUTTICHAI PHAE-NGAM, ONGKARN VANIJAJIVA","doi":"10.13057/biodiv/d240744","DOIUrl":"https://doi.org/10.13057/biodiv/d240744","url":null,"abstract":"Abstract. Nitiworakarn S, Phae-Ngam W, Vanijajiva O. 2023. Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers. Biodiversitas 24: 4060-4068. Musa cultivars are mostly obtained from crossing between wild diploid subspecies of M. acuminata (A genome) and M. balbisiana (B genome), and they reveal numerous levels of ploidy and genomic constitution. The present study, for the first time, investigated the genetic diversity of 90 Musa cultivars from 17 localities of Chai Badan district in Lopburi Province, Thailand, using start codon targeted (SCoT) markers. The SCoT technique has shown very high repeatability for characterizing Musa cultivars. The result indicated that DNA fingerprints from 30 SCoT primers generated 244 amplicons, among which 238 bands (97.54%) were polymorphic, with a mean of 7.93 polymorphic bands per primer. The average polymorphism information content (PIC) was high, ranging from 0.345 to 0.483, with an average of 0.432. Genetic diversity and its partitioning parameters were calculated and demonstrated that present Musa cultivars maintain relatively high genetic diversity. The unweighted pair-group method with an arithmetic cluster analysis, principal coordinate analysis and STRUCTURE analysis results were the same and showed the clear division of the genotypes into two distinct clusters, which were cluster-I consisted of only the AA genomes and cluster-II comprised of ABB and BB genomes. In conclusion, SCoT markers obtained here showed their manipulation in genetic diversity and the relationship of cultivated Musa, which would be practical for genetic preservation and continuing breeding programs in Thailand and other regions.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135064787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract. Bazarov B, Rajamuradov Z, Safin M, Rajabov A, Khayitov D, Kuziev M, Aminjonov S, Ismayilova M, Kudratov J, Khujabekov M, Khaydarov D. 2023. The productivity, chemical composition and nutritional value of pastures dominated by Artemisia diffusa and Cousinia resinosa in arid lands of southwestern Uzbekistan. Biodiversitas 24: 3916-3923. In recent years, extensive degradation of pastures has been observed all over the world as a result of drastic changes in climate and the increase of anthropogenic factors. As a result, the biological diversity of plant communities in pastures decreases, and specific changes occur in dry mass productivity, nutritional value, and other indicators of plant biomass. Therefore, studies aimed at assessing the condition of the vegetation in pastures are important. This research is one of the first studies of this type aimed at determining the biomass yield of pastures dominated by Artemisia diffusa Krasch. ex Poljako and Cousinia resinosa Juz. at varying elevations in arid areas of the southwestern regions of Uzbekistan, analyzing the chemical composition and nutritional value contained in the plants and evaluating the yield and quality of milk of goats fed with such plants. The results showed that dry mass yield, chemical composition, amount of substance, and nutritional value of the biomass produced by the plants in the pastures were directly influenced by the type of plants that make up the pasture, the elevation of the pastures, and climate factors (i.e., season). Our analysis also showed that the resiproductivity, chemical composition, and nutritional parameters were the highest in the mountain pastures compared to those in the hill and desert pastures. Goats fed by grazing in the pastures produced a higher yield and quality of milk compared to goats fed with conventional rations. The findings of this study explain the diversity of the productivity, chemical composition, nutritional value, and potential of the biomass produced by plants in pastures with dominant species A. diffusa and C. resinosa in increasing goat milk production in arid regions.
摘要巴扎罗夫B, Rajamuradov Z, Safin M, Rajabov A, Khayitov D, Kuziev M, Aminjonov S, Ismayilova M, Kudratov J, Khujabekov M, Khaydarov D. 2023。乌兹别克斯坦西南部干旱区白花蒿和臭臭草为主草场的生产力、化学成分和营养价值。生物多样性24:3916-3923。近年来，由于气候的剧烈变化和人为因素的增加，世界各地的牧场出现了广泛的退化。因此，牧场植物群落的生物多样性下降，植物生物量的干质量生产力、营养价值等指标发生了具体变化。因此，对草场植被状况进行评估具有重要意义。本研究是首次对白花蒿(Artemisia diffusa Krasch)牧草生物量产量进行研究。前Poljako和Cousinia resinosa Juz。在乌兹别克斯坦西南部干旱地区不同海拔的地区，分析了这些植物所含的化学成分和营养价值，并评估了用这些植物喂养的山羊的产奶量和奶质量。结果表明，牧草植物的干质量、化学成分、物质含量和营养价值直接受到牧草类型、牧草海拔和季节等气候因素的影响。分析还表明，与丘陵和荒漠牧草相比，山地牧草的弹性生产力、化学成分和营养参数最高。在牧场放牧的山羊比用常规口粮喂养的山羊产量和奶质量更高。本研究结果解释了干旱地区优势种白花羊草和树脂羊草在提高羊奶产量方面的生产力、化学成分、营养价值和生物量多样性。
{"title":"The productivity, chemical composition and nutritional value of pastures dominated by Artemisia diffusa and Cousinia resinosa in arid lands of southwestern Uzbekistan","authors":"BAXRITDIN BAZAROV, ZAYNITDIN RAJAMURADOV, MARS SAFIN, ASATILLO RAJABOV, DAVRON KHAYITOV, MIRZOXID KUZIEV, SHARIFKUL AMINJONOV, MARKHAMAT ISMAYILOVA, JASUR KUDRATOV, MIRZAAHMAD KHUJABEKOV, DILOVAR KHAYDAROV","doi":"10.13057/biodiv/d240730","DOIUrl":"https://doi.org/10.13057/biodiv/d240730","url":null,"abstract":"Abstract. Bazarov B, Rajamuradov Z, Safin M, Rajabov A, Khayitov D, Kuziev M, Aminjonov S, Ismayilova M, Kudratov J, Khujabekov M, Khaydarov D. 2023. The productivity, chemical composition and nutritional value of pastures dominated by Artemisia diffusa and Cousinia resinosa in arid lands of southwestern Uzbekistan. Biodiversitas 24: 3916-3923. In recent years, extensive degradation of pastures has been observed all over the world as a result of drastic changes in climate and the increase of anthropogenic factors. As a result, the biological diversity of plant communities in pastures decreases, and specific changes occur in dry mass productivity, nutritional value, and other indicators of plant biomass. Therefore, studies aimed at assessing the condition of the vegetation in pastures are important. This research is one of the first studies of this type aimed at determining the biomass yield of pastures dominated by Artemisia diffusa Krasch. ex Poljako and Cousinia resinosa Juz. at varying elevations in arid areas of the southwestern regions of Uzbekistan, analyzing the chemical composition and nutritional value contained in the plants and evaluating the yield and quality of milk of goats fed with such plants. The results showed that dry mass yield, chemical composition, amount of substance, and nutritional value of the biomass produced by the plants in the pastures were directly influenced by the type of plants that make up the pasture, the elevation of the pastures, and climate factors (i.e., season). Our analysis also showed that the resiproductivity, chemical composition, and nutritional parameters were the highest in the mountain pastures compared to those in the hill and desert pastures. Goats fed by grazing in the pastures produced a higher yield and quality of milk compared to goats fed with conventional rations. The findings of this study explain the diversity of the productivity, chemical composition, nutritional value, and potential of the biomass produced by plants in pastures with dominant species A. diffusa and C. resinosa in increasing goat milk production in arid regions.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135602892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BUDIAWATI S. ISKANDAR, BUDI IRAWAN, DEDE MULYANTO, JOHAN ISKANDAR, AFDILAH AFINANDA, BUDI RAJAB
Abstract. Iskandar BS, Irawan B, Mulyanto D, Iskandar J, Afinanda A, Rajab B. 2023. Gastronomic ethnobotany of traditional vegetables among the Sundanese in rural West Java, Indonesia. Biodiversitas 24: 3932-3950. Sundanese people in West Java have been known for their tradition of consuming various vegetable plants (Lalab). This study aims to elucidate the Traditional Ecological Knowledge (TEK) on various vegetable plants, folk classification, and use and processing of vegetable plants for family use in the rural Sundanese community in Cijambu Village, Tanjungsari Sub-district, Sumedang District, West Java, Indonesia. This study employed mixed qualitative and quantitative methods with an ethnobotanical and gastronomic approach. The results showed that 67 different local varieties (landraces) of vegetable plants were documented from 65 species, representing 56 genera and 27 families that the Sundanese people of Cijambu commonly use. These vegetable plants can be classified into three categories: vegetable source, part used, and use and processing of vegetables. The people obtain their vegetables from various sources, such as their homegardens, gardens, rice fields, Perhutani agroforests, and other non-farming sources, including markets, small village shops, and vegetable sellers. The parts of vegetable plants usually consumed by the people of Cijambu Village can be divided into eight parts: the bulb, flower, fruit, leaf, rhizome, root/tuber, seed, and shoot. The vegetable plants are traditionally consumed as fresh raw salad and cooked vegetables, spices, and at least 12 main or basic types of Sundanese food meals, namely various Acar, Asinan, Buntil, Karedok, Lodeh, Lotek, Rujak, Sambel, Sayur/Angeun, Semur, Tumis/Oseng, and Urab with a total of 58 kinds of food meals. At the same time, various Sundanese chili sauces (Sambel) were documented in at least 14 types. Based on ethnobotanical gastronomy, the Sundanese community's cultural practice of consuming Lalab results from a complex interaction between human communities, food, and their local environment.
摘要依斯干达BS, Irawan B, Mulyanto D，依斯干达J, Afinanda A, Rajab B. 2023。印尼西爪哇农村巽他人传统蔬菜的美食民族植物学研究。生物多样性24:3932-3950。西爪哇的巽他人以食用各种蔬菜植物(Lalab)的传统而闻名。本研究旨在了解印度尼西亚西爪哇省苏梅当区丹戎萨里街道Cijambu村Sundanese社区关于各种蔬菜植物的传统生态知识、民间分类以及家庭使用蔬菜植物的利用和加工。本研究采用混合定性和定量方法，结合民族植物学和美食学方法。结果表明，旗jambu巽他人常用的蔬菜植物共有67个不同的地方品种(地方品种)，来自65种，代表56属27科。这些蔬菜植物可分为三类:蔬菜来源、部分利用、蔬菜利用和加工。人们从各种各样的来源获得蔬菜，如他们的家庭菜园、花园、稻田、Perhutani农林业和其他非农业来源，包括市场、小村庄商店和蔬菜销售商。慈jambu村人通常食用的蔬菜可分为球茎、花、果、叶、根茎、根/块茎、种子和芽八个部分。传统上，这些蔬菜被用作新鲜的生沙拉和煮熟的蔬菜、香料，以及至少12种主要或基本类型的Sundanese食物，即各种Acar、Asinan、Buntil、Karedok、Lodeh、Lotek、Rujak、Sambel、Sayur/Angeun、Semur、Tumis/Oseng和Urab，总共58种食物。与此同时，各种各样的巽他辣椒酱(Sambel)被记载为至少14种。基于民族植物学的美食学，巽他人社区消费Lalab的文化实践是人类社区、食物和当地环境之间复杂的相互作用的结果。
{"title":"Gastronomic ethnobotany of traditional vegetables among the Sundanese in rural West Java, Indonesia","authors":"BUDIAWATI S. ISKANDAR, BUDI IRAWAN, DEDE MULYANTO, JOHAN ISKANDAR, AFDILAH AFINANDA, BUDI RAJAB","doi":"10.13057/biodiv/d240732","DOIUrl":"https://doi.org/10.13057/biodiv/d240732","url":null,"abstract":"Abstract. Iskandar BS, Irawan B, Mulyanto D, Iskandar J, Afinanda A, Rajab B. 2023. Gastronomic ethnobotany of traditional vegetables among the Sundanese in rural West Java, Indonesia. Biodiversitas 24: 3932-3950. Sundanese people in West Java have been known for their tradition of consuming various vegetable plants (Lalab). This study aims to elucidate the Traditional Ecological Knowledge (TEK) on various vegetable plants, folk classification, and use and processing of vegetable plants for family use in the rural Sundanese community in Cijambu Village, Tanjungsari Sub-district, Sumedang District, West Java, Indonesia. This study employed mixed qualitative and quantitative methods with an ethnobotanical and gastronomic approach. The results showed that 67 different local varieties (landraces) of vegetable plants were documented from 65 species, representing 56 genera and 27 families that the Sundanese people of Cijambu commonly use. These vegetable plants can be classified into three categories: vegetable source, part used, and use and processing of vegetables. The people obtain their vegetables from various sources, such as their homegardens, gardens, rice fields, Perhutani agroforests, and other non-farming sources, including markets, small village shops, and vegetable sellers. The parts of vegetable plants usually consumed by the people of Cijambu Village can be divided into eight parts: the bulb, flower, fruit, leaf, rhizome, root/tuber, seed, and shoot. The vegetable plants are traditionally consumed as fresh raw salad and cooked vegetables, spices, and at least 12 main or basic types of Sundanese food meals, namely various Acar, Asinan, Buntil, Karedok, Lodeh, Lotek, Rujak, Sambel, Sayur/Angeun, Semur, Tumis/Oseng, and Urab with a total of 58 kinds of food meals. At the same time, various Sundanese chili sauces (Sambel) were documented in at least 14 types. Based on ethnobotanical gastronomy, the Sundanese community's cultural practice of consuming Lalab results from a complex interaction between human communities, food, and their local environment.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135602518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract. Boonsrangsom T, Fuenghoi C, Premjet D, Suvittawat K, Ratanasut K, Sujipuli K. 2023. Genetic relationships and genome verification of Thai banana cultivars using Random Amplification of Polymorphic DNA (RAPD) markers. Biodiversitas 24: 3758-3765. Edible bananas and plantains, belonging to the family Musaceae, genus Musa, represent one of the most important fruit crops, with an annual production of more than 65 million tons worldwide. Bananas have several hybrid variations since they are descended from the two species Musa acuminata Colla (AA genome) and Musa balbisiana Colla (BB genome). Different morphological traits divide almost hybrid bananas into various genomic groupings. Banana genome categorization and identification, however, have always been challenging issues. This study aimed to assess the genetic relationships and verify the genomes of Thai banana cultivars using random amplification of polymorphic DNA (RAPD) markers. Using the 15 selected RAPD markers, 149 RAPD bands were found, with sizes ranging from 0.2 to 3.2 kb, and 88.6% were polymorphic. Polymorphic information content (PIC) values ranged from 0.18 to 0.42, averaging 0.30. Based on the Jaccard coefficient, the unweighted pair-group method arithmetic average (UPGMA) analysis showed that the banana samples had a similarity range of 0.27 to 1.00 with a mean of 0.56, demonstrating an abundance of viability across six banana genomes. The dendrogram generated from RAPD data revealed that all 18 Musa samples could be divided into two main groups (Group I and II). Three additional subgroups were created for each primary group (A, B, and C). The accurate identification and genetic data on the available genetic resources for bananas will be beneficial for breeding and conservation programs.
{"title":"Genetic relationships and genome verification of Thai banana cultivars using Random Amplification of Polymorphic DNA (RAPD) markers","authors":"THANITA BOONSRANGSOM, CHATTHIDAPHON FUENGHOI, DUANGPORN PREMJET, KUNLAYANEE SUVITTAWAT, KUMROP RATANASUT, KAWEE SUJIPULI","doi":"10.13057/biodiv/d240713","DOIUrl":"https://doi.org/10.13057/biodiv/d240713","url":null,"abstract":"Abstract. Boonsrangsom T, Fuenghoi C, Premjet D, Suvittawat K, Ratanasut K, Sujipuli K. 2023. Genetic relationships and genome verification of Thai banana cultivars using Random Amplification of Polymorphic DNA (RAPD) markers. Biodiversitas 24: 3758-3765. Edible bananas and plantains, belonging to the family Musaceae, genus Musa, represent one of the most important fruit crops, with an annual production of more than 65 million tons worldwide. Bananas have several hybrid variations since they are descended from the two species Musa acuminata Colla (AA genome) and Musa balbisiana Colla (BB genome). Different morphological traits divide almost hybrid bananas into various genomic groupings. Banana genome categorization and identification, however, have always been challenging issues. This study aimed to assess the genetic relationships and verify the genomes of Thai banana cultivars using random amplification of polymorphic DNA (RAPD) markers. Using the 15 selected RAPD markers, 149 RAPD bands were found, with sizes ranging from 0.2 to 3.2 kb, and 88.6% were polymorphic. Polymorphic information content (PIC) values ranged from 0.18 to 0.42, averaging 0.30. Based on the Jaccard coefficient, the unweighted pair-group method arithmetic average (UPGMA) analysis showed that the banana samples had a similarity range of 0.27 to 1.00 with a mean of 0.56, demonstrating an abundance of viability across six banana genomes. The dendrogram generated from RAPD data revealed that all 18 Musa samples could be divided into two main groups (Group I and II). Three additional subgroups were created for each primary group (A, B, and C). The accurate identification and genetic data on the available genetic resources for bananas will be beneficial for breeding and conservation programs.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135602525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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