ANI MARDIASTUTI, YENI A. MULYANI, ANDRIE AKBAR, BASUKI WICAKSONO, TIARA RAHMAWATI, YULIA P. WULANDARI, WARSAJAYA WARSAJAYA
Abstract. Mardiastuti A, Mulyani YA, Akbar A, Wicaksono B, Rahmawati T, Wulandari YP, Warsajaya. 2023. The use of drone to study the nesting behavior of milky stork: Some preliminary observations. Biodiversitas 24: 4549-4557. The use of a drone to census waterbirds has been increasingly popular. However, study on the waterbird’s behavioral response to the drone was still limited. The objective of this study is to reveal the response of the breeding milky stork Mycteria cinerea to the drone as a new and safer tool for collecting breeding behavior data. Factors that influence the success of the observation by using a drone were also identified and a set of recommendations was formulated. The research was conducted during the breeding season of the milky stork in Pulau Rambut, a small island in Jakarta Bay, between December 2022 and March 2023. Three types of drones were tested in the pilot study, and eventually, a small manually piloted drone (Syma X25 Pro) was found to be more appropriate for behavioral study. The bird response to the drone (no, neutral, temporary, negative response) was observed through a combination of various distances and angles of the drone to the nest trees. Despite the weather obstacles, this study suggested that the milky stork mostly (17 out of 20 trials) showed no response or neutral response to the drone. Some temporary responses were detected due to some accidental events, including a sudden launch of the drone. In conclusion, a small-size, easy-to-maneuver drone that produces low noise and mild wind can be used by using a manual pilot mode to study the breeding behavior of milky storks or other similar waterbird species.
摘要马迪阿斯图提A, Mulyani YA, Akbar A, Wicaksono B, Rahmawati T, Wulandari YP, Warsajaya. 2023。用无人机研究白鹳的筑巢行为:一些初步观察。生物多样性,24:4549-4557。使用无人机对水鸟进行普查越来越受欢迎。然而,对水鸟对无人机的行为反应的研究仍然有限。本研究的目的是揭示作为一种新的、更安全的采集育种行为数据工具的无人机对繁殖中的白鹳灰分枝杆菌的反应。还确定了影响使用无人机观测成功的因素,并制定了一套建议。这项研究是在2022年12月至2023年3月期间,在雅加达湾的一个小岛普兰布特岛(Pulau Rambut)的白鹳繁殖季节进行的。在初步研究中测试了三种类型的无人机,最终发现小型手动驾驶无人机(Syma X25 Pro)更适合用于行为研究。鸟类对无人机的反应(无,中性,临时,消极的反应)通过无人机到巢树的不同距离和角度的组合来观察。尽管有天气障碍,这项研究表明,大多数乳鹤(20次试验中的17次)对无人机没有反应或中性反应。由于一些意外事件,包括无人机的突然发射,检测到一些临时反应。综上所述,可以使用一种小型、易于操作、噪音低、风力温和的无人机,通过手动驾驶模式来研究乳鹤或其他类似水鸟物种的繁殖行为。
{"title":"The use of drone to study the nesting behavior of milky stork: Some preliminary observations","authors":"ANI MARDIASTUTI, YENI A. MULYANI, ANDRIE AKBAR, BASUKI WICAKSONO, TIARA RAHMAWATI, YULIA P. WULANDARI, WARSAJAYA WARSAJAYA","doi":"10.13057/biodiv/d240836","DOIUrl":"https://doi.org/10.13057/biodiv/d240836","url":null,"abstract":"Abstract. Mardiastuti A, Mulyani YA, Akbar A, Wicaksono B, Rahmawati T, Wulandari YP, Warsajaya. 2023. The use of drone to study the nesting behavior of milky stork: Some preliminary observations. Biodiversitas 24: 4549-4557. The use of a drone to census waterbirds has been increasingly popular. However, study on the waterbird’s behavioral response to the drone was still limited. The objective of this study is to reveal the response of the breeding milky stork Mycteria cinerea to the drone as a new and safer tool for collecting breeding behavior data. Factors that influence the success of the observation by using a drone were also identified and a set of recommendations was formulated. The research was conducted during the breeding season of the milky stork in Pulau Rambut, a small island in Jakarta Bay, between December 2022 and March 2023. Three types of drones were tested in the pilot study, and eventually, a small manually piloted drone (Syma X25 Pro) was found to be more appropriate for behavioral study. The bird response to the drone (no, neutral, temporary, negative response) was observed through a combination of various distances and angles of the drone to the nest trees. Despite the weather obstacles, this study suggested that the milky stork mostly (17 out of 20 trials) showed no response or neutral response to the drone. Some temporary responses were detected due to some accidental events, including a sudden launch of the drone. In conclusion, a small-size, easy-to-maneuver drone that produces low noise and mild wind can be used by using a manual pilot mode to study the breeding behavior of milky storks or other similar waterbird species.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136363587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MOHAMMAD UBAIDILLAH, NURHALIZA THAMRIN, FADIA INTAN CAHYANI, DINA FITRIYAH
Abstract. Ubaidillah M, Thamrin N, Cahyani FI, Fitriyah D. 2023. Bioremediation potential of rhizosphere bacterial consortium in lead (Pb) contaminated rice plants. Biodiversitas 24: 4566-4571. Lead (Pb) is one of the toxic heavy metals detected in various chemical fertilizers and pesticides used in Indonesia. These chemicals can increase plant lead (Pb) accumulation and affect other organisms through its circulation with food chain. Excess amounts of Pb in soil may be remediated using different approaches such as bioremediation. Rhizosphere bacteria can be used as bioremediation agents to reduce Pb accumulation in plants and also increase plant growth. Actinomycetes, Azotobacter sp., Azospirillum sp., Rhizobium sp., Pseudomonas sp., Lactobacillus sp., Bacillus sp., and Streptomyces sp. are some of the several bacteria that can reduce lead (Pb) accumulation in plants by the ability to sequester Pb, change its oxidation state, or enable Pb to precipitate. This research was conducted to determine the ability of the rhizosphere bacterial consortium to prevent lead (Pb) accumulation in rice plants. This study confirmed that the application of rhizosphere bacterial consortium to rice plants contaminated with lead (Pb) can reduce the plant's lead (Pb) content by up to 45%. The use of a bacterial consortium did not significantly affect the growth parameters of the plant but increased root length by 41%, fresh weight by 32%, dry weight by 26%, and chlorophyll content by 25%, in rice plants contaminated with Pb.
{"title":"Bioremediation potential of rhizosphere bacterial consortium in lead (Pb) contaminated rice plants","authors":"MOHAMMAD UBAIDILLAH, NURHALIZA THAMRIN, FADIA INTAN CAHYANI, DINA FITRIYAH","doi":"10.13057/biodiv/d240838","DOIUrl":"https://doi.org/10.13057/biodiv/d240838","url":null,"abstract":"Abstract. Ubaidillah M, Thamrin N, Cahyani FI, Fitriyah D. 2023. Bioremediation potential of rhizosphere bacterial consortium in lead (Pb) contaminated rice plants. Biodiversitas 24: 4566-4571. Lead (Pb) is one of the toxic heavy metals detected in various chemical fertilizers and pesticides used in Indonesia. These chemicals can increase plant lead (Pb) accumulation and affect other organisms through its circulation with food chain. Excess amounts of Pb in soil may be remediated using different approaches such as bioremediation. Rhizosphere bacteria can be used as bioremediation agents to reduce Pb accumulation in plants and also increase plant growth. Actinomycetes, Azotobacter sp., Azospirillum sp., Rhizobium sp., Pseudomonas sp., Lactobacillus sp., Bacillus sp., and Streptomyces sp. are some of the several bacteria that can reduce lead (Pb) accumulation in plants by the ability to sequester Pb, change its oxidation state, or enable Pb to precipitate. This research was conducted to determine the ability of the rhizosphere bacterial consortium to prevent lead (Pb) accumulation in rice plants. This study confirmed that the application of rhizosphere bacterial consortium to rice plants contaminated with lead (Pb) can reduce the plant's lead (Pb) content by up to 45%. The use of a bacterial consortium did not significantly affect the growth parameters of the plant but increased root length by 41%, fresh weight by 32%, dry weight by 26%, and chlorophyll content by 25%, in rice plants contaminated with Pb.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135364036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LIANAH LIANAH, NIKEN KUSUMARINI, MUTISTA HAFSHAH, KRISANTINI KRISANTINI, ANI KURNIAWATI, MILYA URFA AHMAD
Abstract. Lianah L, Kusumarini N, Hafshah M, Krisantini K, Kurniawati A, Ahmad MA. 2023. Chemical characterization of mint (Mentha spp.) germplasm from Central Java, Indonesia. Biodiversitas 24: 4307-4313. The main objective of this work was to evaluate and compare the leaf production and oil content of the four mint genotypes (Mentha spp.) from Central Java, Indonesia. Four genotypes were collected from Rejosari Village, Kudus District, Central Java, Indonesia, namely spearmint (Mentha spicata), peppermint (Mentha x piperita), orange mint (Mentha x piperita ‘Orange’) and chocolate mint (Mentha x piperita ‘Chocolate’). Essential oil extraction was carried out by steam distillation. Identification of the chemical compounds of the oil and their relative percentage were analyzed using GC-MS. Leaves productivity ranged between 500-1000 kg/ha, and essential oil content from 0.4 to 1.36%. The major essential oil constituents detected were 1,8-cineole, carvone, limonene, linalool, linalyl acetate, menthol, menthone, menthyl acetate, and piperitenone oxide. Chocolate mint had the highest leaf production and the highest content of essential oil (4.17%) and menthone yield (28%) compared to the other genotypes.
摘要Lianah L, Kusumarini N, Hafshah M, Krisantini K, Kurniawati A, Ahmad MA。2023. 印度尼西亚中爪哇薄荷(薄荷属)种质的化学特性。生物多样性24:4307-4313。本研究的主要目的是评价和比较来自印度尼西亚中爪哇的四种薄荷基因型(薄荷属)的叶片产量和含油量。从印度尼西亚中爪哇省Kudus地区的Rejosari村收集了4种基因型,即绿薄荷(薄荷)、薄荷(薄荷x胡椒)、橙薄荷(薄荷x胡椒'橙')和巧克力薄荷(薄荷x胡椒'巧克力')。采用水蒸气蒸馏法提取精油。采用气相色谱-质谱联用技术对其化学成分进行了鉴定,并对其相对含量进行了分析。叶片产量在500-1000公斤/公顷之间,精油含量在0.4 - 1.36%之间。主要精油成分为1,8-桉树脑、香芹酮、柠檬烯、芳樟醇、乙酸芳樟醇、薄荷醇、薄荷酮、乙酸薄荷酯和氧化胡椒烯酮。与其他基因型相比,巧克力薄荷的叶片产量最高,精油含量最高(4.17%),薄荷酮产量最高(28%)。
{"title":"Chemical characterization of mint (Mentha spp.) germplasm from Central Java, Indonesia","authors":"LIANAH LIANAH, NIKEN KUSUMARINI, MUTISTA HAFSHAH, KRISANTINI KRISANTINI, ANI KURNIAWATI, MILYA URFA AHMAD","doi":"10.13057/biodiv/d240812","DOIUrl":"https://doi.org/10.13057/biodiv/d240812","url":null,"abstract":"Abstract. Lianah L, Kusumarini N, Hafshah M, Krisantini K, Kurniawati A, Ahmad MA. 2023. Chemical characterization of mint (Mentha spp.) germplasm from Central Java, Indonesia. Biodiversitas 24: 4307-4313. The main objective of this work was to evaluate and compare the leaf production and oil content of the four mint genotypes (Mentha spp.) from Central Java, Indonesia. Four genotypes were collected from Rejosari Village, Kudus District, Central Java, Indonesia, namely spearmint (Mentha spicata), peppermint (Mentha x piperita), orange mint (Mentha x piperita ‘Orange’) and chocolate mint (Mentha x piperita ‘Chocolate’). Essential oil extraction was carried out by steam distillation. Identification of the chemical compounds of the oil and their relative percentage were analyzed using GC-MS. Leaves productivity ranged between 500-1000 kg/ha, and essential oil content from 0.4 to 1.36%. The major essential oil constituents detected were 1,8-cineole, carvone, limonene, linalool, linalyl acetate, menthol, menthone, menthyl acetate, and piperitenone oxide. Chocolate mint had the highest leaf production and the highest content of essential oil (4.17%) and menthone yield (28%) compared to the other genotypes.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135364211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
IRNANDA AIKO FIFI DJUUNA, NUNANG LAMAEK MAY, SITI HAJAR KUBANGUN, MARIA MASSORA, SOBA WILLEM AIBINI
Abstract. Djuuna IAF, May NL, Kubangun SH, Massora M, Aibini SW. 2023. Distribution of Arbuscular Mycorrhiza Fungi (AM Fungi) in different plants within tailings deposition areas of Freeport Indonesia, Timika, Central Papua, Indonesia. Biodiversitas 24: 4515-4522. This study investigated the presence of Arbuscular Mycorrhiza Fungi (AM Fungi) in various vegetation types within tailings deposition areas in Freeport Timika, Papua. Soil and root samples were collected from 41 sampling locations, representing 28 types of vegetation growing in these areas. AM Fungi spores were extracted using the wet sieving technique, while root samples were cleaned, stained, and analyzed for the percentage of root infection using the gridlines method. Some soil characteristics were also analyzed, including pH (H2O), moisture content, organic carbon (C), Total nitrogen (N) and phosphorus (P), and soil texture. The study showed low total soil N and P levels, with organic C ranging from low to high and a pH range from 6 to 6.7. The spore number ranged from 2 to 20 spores/10 g soil, and the percentage of root colonization varied from 17 to 89.5%. The highest number of spores (20 spores/10 g soil) was found in the rhizosphere of Homalanthus sp. and Polyalthia glauca, while the highest percentage of root colonization (89.5%) was observed under Pandanus sp. at Upper ADA. Notably, the highest number of spores did not correspond to the highest percentage of infected roots. Additionally, four genera of AM Fungi, namely Glomus, Acaulospora, Scuttelospora and Gigaspora, were found to thrive in tailings areas and have the potential to be developed as biofertilizers for tailings rehabilitation programs.
{"title":"Distribution of Arbuscular Mycorrhiza Fungi (AM Fungi) in different plants within tailings deposition areas of Freeport Indonesia, Timika, Central Papua, Indonesia","authors":"IRNANDA AIKO FIFI DJUUNA, NUNANG LAMAEK MAY, SITI HAJAR KUBANGUN, MARIA MASSORA, SOBA WILLEM AIBINI","doi":"10.13057/biodiv/d240832","DOIUrl":"https://doi.org/10.13057/biodiv/d240832","url":null,"abstract":"Abstract. Djuuna IAF, May NL, Kubangun SH, Massora M, Aibini SW. 2023. Distribution of Arbuscular Mycorrhiza Fungi (AM Fungi) in different plants within tailings deposition areas of Freeport Indonesia, Timika, Central Papua, Indonesia. Biodiversitas 24: 4515-4522. This study investigated the presence of Arbuscular Mycorrhiza Fungi (AM Fungi) in various vegetation types within tailings deposition areas in Freeport Timika, Papua. Soil and root samples were collected from 41 sampling locations, representing 28 types of vegetation growing in these areas. AM Fungi spores were extracted using the wet sieving technique, while root samples were cleaned, stained, and analyzed for the percentage of root infection using the gridlines method. Some soil characteristics were also analyzed, including pH (H2O), moisture content, organic carbon (C), Total nitrogen (N) and phosphorus (P), and soil texture. The study showed low total soil N and P levels, with organic C ranging from low to high and a pH range from 6 to 6.7. The spore number ranged from 2 to 20 spores/10 g soil, and the percentage of root colonization varied from 17 to 89.5%. The highest number of spores (20 spores/10 g soil) was found in the rhizosphere of Homalanthus sp. and Polyalthia glauca, while the highest percentage of root colonization (89.5%) was observed under Pandanus sp. at Upper ADA. Notably, the highest number of spores did not correspond to the highest percentage of infected roots. Additionally, four genera of AM Fungi, namely Glomus, Acaulospora, Scuttelospora and Gigaspora, were found to thrive in tailings areas and have the potential to be developed as biofertilizers for tailings rehabilitation programs.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135364032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LUQMAN QURATA AINI, IRISA TRIANTI, SHOLIKAH WIDYANITTA RACHMAWATI, ANTON MEILUS PUTRA, NABILLA ALYA ANASTASYA, ADI SETIAWAN
Abstract. Aini LQ, Trianti I, Rachmawati SW, Putra AM, Anastasya NA, Setiawan A. 2023. In vitro characterization of UB Forest (Malang, Indonesia) indigenous bacteria as plant growth promoting bacteria (PGPB). Biodiversitas 24: 4558-4565. UB Forest, an educational forest of Universitas Brawijaya in Malang, East Java, has a mega-biodiversity of microbial germplasm. Previously, we obtained several UB Forest indigenous bacterial isolates and tested them on various plant commodities. However, the comprehensive characterization of the bacteria to produce IAA hormone, to overcome biotic and abiotic stresses as well as their potency as biofertilizers in vitro has not been carried out yet. In this study, we conducted in vitro assays to elucidate the potency of UB Forest indigenous bacterial isolates as Plant Growth Promoting Bacteria (PGPB). The molecular identification showed that the bacterial strains were dominated by the Pseudomonads, namely Pseudomonas versuta UB-36, P. aeruginosa UB-52, P. lundensis UB-53, P. migulae UB-54, and P. koreensis UB-62. Other strains were Enterococcus gallinarum UB-55 and Lysinibacillus fusiformis UB-64. Of the 7 bacterial strains, only 3 could inhibit Xanthomonas campestris. All bacterial strains were able to produce IAA, whereas five bacteria can solubilize phosphate, six bacteria can fix nitrogen, and four bacteria have both activities. All bacterial strains can grow at pH 5-6, salinity 5-15%, temperature 60°C, and 15% polyethylene glycol (PEG) drought stress media. The results suggested that the UB Forest indigenous bacterial strains have a role as plant growth-promoting bacteria (PGPB) and are expected to support the growth of plants grown under biotic and abiotic stress conditions.
摘要Aini LQ, Trianti I, Rachmawati SW, Putra AM, Anastasya NA, Setiawan A. 2023。UB森林(Malang, Indonesia)原生细菌作为植物生长促进菌(PGPB)的体外鉴定生物多样性,24:4558-4565。UB森林是东爪哇玛琅的布拉维加亚大学的一个教育森林,拥有丰富的微生物种质资源。以前,我们获得了几个UB森林本地细菌分离株,并在各种植物商品上进行了测试。然而,目前还没有对产生IAA激素、克服生物和非生物胁迫的细菌及其作为生物肥料的体外效能进行全面的表征。在这项研究中,我们进行了体外实验来阐明UB森林本土细菌分离物作为植物生长促进细菌(PGPB)的效力。分子鉴定结果表明,菌株以假单胞菌为主,分别为反假单胞菌UB-36、铜绿假单胞菌UB-52、隆德假单胞菌UB-53、乳沟假单胞菌UB-54和韩国假单胞菌UB-62。其他菌株为鸡肠球菌UB-55和梭状芽胞杆菌UB-64。7株细菌中,仅有3株能抑制油菜黄单胞菌。所有菌株都能产生IAA,其中5株细菌能溶解磷酸盐,6株细菌能固定氮,4株细菌同时具有这两种活性。所有菌株均能在pH 5-6、盐度5-15%、温度60℃和15%聚乙二醇(PEG)干旱胁迫介质下生长。结果表明,UB森林本地菌株具有植物生长促进菌(PGPB)的作用,有望在生物和非生物胁迫条件下支持植物的生长。
{"title":"In vitro characterization of UB Forest (Malang, Indonesia) indigenous bacteria as plant growth promoting bacteria (PGPB)","authors":"LUQMAN QURATA AINI, IRISA TRIANTI, SHOLIKAH WIDYANITTA RACHMAWATI, ANTON MEILUS PUTRA, NABILLA ALYA ANASTASYA, ADI SETIAWAN","doi":"10.13057/biodiv/d240837","DOIUrl":"https://doi.org/10.13057/biodiv/d240837","url":null,"abstract":"Abstract. Aini LQ, Trianti I, Rachmawati SW, Putra AM, Anastasya NA, Setiawan A. 2023. In vitro characterization of UB Forest (Malang, Indonesia) indigenous bacteria as plant growth promoting bacteria (PGPB). Biodiversitas 24: 4558-4565. UB Forest, an educational forest of Universitas Brawijaya in Malang, East Java, has a mega-biodiversity of microbial germplasm. Previously, we obtained several UB Forest indigenous bacterial isolates and tested them on various plant commodities. However, the comprehensive characterization of the bacteria to produce IAA hormone, to overcome biotic and abiotic stresses as well as their potency as biofertilizers in vitro has not been carried out yet. In this study, we conducted in vitro assays to elucidate the potency of UB Forest indigenous bacterial isolates as Plant Growth Promoting Bacteria (PGPB). The molecular identification showed that the bacterial strains were dominated by the Pseudomonads, namely Pseudomonas versuta UB-36, P. aeruginosa UB-52, P. lundensis UB-53, P. migulae UB-54, and P. koreensis UB-62. Other strains were Enterococcus gallinarum UB-55 and Lysinibacillus fusiformis UB-64. Of the 7 bacterial strains, only 3 could inhibit Xanthomonas campestris. All bacterial strains were able to produce IAA, whereas five bacteria can solubilize phosphate, six bacteria can fix nitrogen, and four bacteria have both activities. All bacterial strains can grow at pH 5-6, salinity 5-15%, temperature 60°C, and 15% polyethylene glycol (PEG) drought stress media. The results suggested that the UB Forest indigenous bacterial strains have a role as plant growth-promoting bacteria (PGPB) and are expected to support the growth of plants grown under biotic and abiotic stress conditions.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135452918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
RAHMAD LINGGA, SUDIRMAN ADIBRATA, OCCA ROANISCA, SIPRIYADI SIPRIYADI, RISKY HADI WIBOWO, AHMAD ARSYADI
Abstract. Lingga R, Adibrata S, Roanisca O, Sipriyadi, Wibowo RH, Arsyadi. 2023. Probiotics potential of lactic acid bacteria isolated from Slender Walking Catfish (Clarias nieuhofii). Biodiversitas 24: 4572-4580. Probiotics are living microorganisms or bioactive agents that positively impact animal digestion. They have successfully isolated from various sources. Recently, we isolated and characterized lactic acid bacteria (LAB) from slender walking fish (Clarias nieuhofii Valenciennes, 1840). The fish sample was obtained from Batu Rusa and Paya Benua Rivers in Bangka Island, Indonesia. LAB was isolated from the fish intestine using the pour plate method. The isolated LAB was then characterized based on their phenotypic traits, biochemical properties, and the 16S rRNA gene identification. The chosen isolates were tested to determine their ability to produce lactic acid, hemolytic and antibacterial activity, and antibiotic resistance. All isolates have the characteristic of rod-shaped and short rod-shaped cells with Gram-positive properties. Isolate KP1 showed the number of populations (2.89 x 107 CFU/mL) and lactic acid production with a concentration of 1.85%. All isolates exhibited no hemolysis activity and displayed sensitivity to antibiotics. The twelve lactic acid bacteria formed clear zones against Staphylococcus aureus and Escherichia coli. The results of the 16S rRNA gene identification indicated that isolates KB4, KB7, KB8, and KP1 belong to Lactobacillus vaginalis, Lactobacillus fermentum, Limosilactobacillus fermentum, and Levilactobacillus brevis, respectively. Lactic acid bacteria isolated from slender walking fish exhibited potential probiotic traits.
{"title":"Probiotics potential of lactic acid bacteria isolated from Slender Walking Catfish (Clarias nieuhofii)","authors":"RAHMAD LINGGA, SUDIRMAN ADIBRATA, OCCA ROANISCA, SIPRIYADI SIPRIYADI, RISKY HADI WIBOWO, AHMAD ARSYADI","doi":"10.13057/biodiv/d240839","DOIUrl":"https://doi.org/10.13057/biodiv/d240839","url":null,"abstract":"Abstract. Lingga R, Adibrata S, Roanisca O, Sipriyadi, Wibowo RH, Arsyadi. 2023. Probiotics potential of lactic acid bacteria isolated from Slender Walking Catfish (Clarias nieuhofii). Biodiversitas 24: 4572-4580. Probiotics are living microorganisms or bioactive agents that positively impact animal digestion. They have successfully isolated from various sources. Recently, we isolated and characterized lactic acid bacteria (LAB) from slender walking fish (Clarias nieuhofii Valenciennes, 1840). The fish sample was obtained from Batu Rusa and Paya Benua Rivers in Bangka Island, Indonesia. LAB was isolated from the fish intestine using the pour plate method. The isolated LAB was then characterized based on their phenotypic traits, biochemical properties, and the 16S rRNA gene identification. The chosen isolates were tested to determine their ability to produce lactic acid, hemolytic and antibacterial activity, and antibiotic resistance. All isolates have the characteristic of rod-shaped and short rod-shaped cells with Gram-positive properties. Isolate KP1 showed the number of populations (2.89 x 107 CFU/mL) and lactic acid production with a concentration of 1.85%. All isolates exhibited no hemolysis activity and displayed sensitivity to antibiotics. The twelve lactic acid bacteria formed clear zones against Staphylococcus aureus and Escherichia coli. The results of the 16S rRNA gene identification indicated that isolates KB4, KB7, KB8, and KP1 belong to Lactobacillus vaginalis, Lactobacillus fermentum, Limosilactobacillus fermentum, and Levilactobacillus brevis, respectively. Lactic acid bacteria isolated from slender walking fish exhibited potential probiotic traits.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135452919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MOHAMMAD MAHMUDI, SULASTRI ARSAD, FIKA FITRIANESIA, SAFRIZAL FIKRI RAMADHAN, ABDUR RAHMAN ARIF, FRIZCA RANIA SAVITRI, ADELITA ASMO DEWINTA, ANINDYA DIVANATA ONGKOSONGO, MUHAMMAD MUSA, EVELLIN DEWI LUSIANA
Abstract. Mahmudi M, Arsad S, Fitrianesia F, Ramadhan SF, Arif AR, Savitri FR, Dewinta AA, Ongkosongo AD, Musa M, Lusiana ED. 2023. Microalgae diversity in varying habitat characteristics in Pasuruan and Sidoarjo coastal areas, East Java, Indonesia. Biodiversitas 24: 4418-4426. Microalgae are microscopic eukaryotic organisms which live in various types of habitats. The purpose of this study was to identify the types and the abundance of microalgae in several sub-habitats, namely sediments, mangroves, water columns, and artificial substrates; and to analyze the environmental factors that affect the abundance of microalgae. The research was conducted in several sites in the coastal areas in Pasuruan and Sidoarjo, East Java, Indonesia. A quantitative descriptive method was applied with data collection used a purposive sampling method. Samples were collected using net for planktonic microalgae and using sampling plot for epiphytic and benthic microalgae. The microalgae were grouped using NMDS (non-metric multidimensional scaling) and the relationships between microalgae abundance and water quality parameters were analyzed using CCA (canonical correspondence analysis). The results show that Bacillariophyceae, Cyanophyceae, and Chlorophyceae classes were found in all study sites, but Trebouxiophyceae and Dinophyceae were only found in Pasuruan Beach. The highest abundance of microalgae was found in sediment habitat in Wughoyo Beach, Sidoarjo with 706,605 ind. cm-2. The diversity, the evenness, and the dominance index in both coastal areas ranged from 1.43-2.61; 0.71-0.96; and 0.06-0.27, respectively. Similarity analysis using the NMDS showed that there was no similarity among the three sites, suggesting each site had high microalgal variation. The CCA analysis showed that Bacillariophyceae were found in all sites, indicating its high adaptability. The results of this analysis imply that particular habitat has a unique microalgae diversity, thus preserving diverse habitat types is important.
摘要Mahmudi M, Arsad S, Fitrianesia F, Ramadhan SF, Arif AR, Savitri FR, Dewinta AA, Ongkosongo AD, Musa M, luusiana ED. 2023。印度尼西亚东爪哇Pasuruan和Sidoarjo沿海地区不同生境特征的微藻多样性。生物多样性24:4418-4426。微藻是一种微小的真核生物,生活在各种类型的栖息地。本研究的目的是确定沉积物、红树林、水柱和人工基质中微藻的类型和丰度;并分析影响微藻丰度的环境因素。这项研究是在印度尼西亚东爪哇Pasuruan和Sidoarjo沿海地区的几个地点进行的。采用定量描述方法,采用目的抽样法收集数据。浮游微藻采用网捕法采集,附生微藻和底栖微藻采用样地采集。采用非度量多维尺度(NMDS)对微藻进行分组,并采用典型对应分析(CCA)分析微藻丰度与水质参数的关系。结果表明:在所有研究点均发现硅藻纲、蓝藻纲和绿藻纲,但仅在帕苏鲁滩发现海藻纲和藻纲。微藻丰度最高的是Sidoarjo的Wughoyo海滩沉积物栖息地,为706,605 ind.cm -2。多样性、均匀度和优势度指数在1.43 ~ 2.61之间;0.71 - -0.96;和0.06-0.27。NMDS相似性分析显示,3个站点之间没有相似性,说明每个站点的微藻差异较大。CCA分析显示,在所有地点均发现了硅藻门,表明其适应性强。这一分析结果表明,特定的生境具有独特的微藻多样性,因此保护不同的生境类型是重要的。
{"title":"Microalgae diversity in varying habitat characteristics in Pasuruan and Sidoarjo coastal areas, East Java, Indonesia","authors":"MOHAMMAD MAHMUDI, SULASTRI ARSAD, FIKA FITRIANESIA, SAFRIZAL FIKRI RAMADHAN, ABDUR RAHMAN ARIF, FRIZCA RANIA SAVITRI, ADELITA ASMO DEWINTA, ANINDYA DIVANATA ONGKOSONGO, MUHAMMAD MUSA, EVELLIN DEWI LUSIANA","doi":"10.13057/biodiv/d240823","DOIUrl":"https://doi.org/10.13057/biodiv/d240823","url":null,"abstract":"Abstract. Mahmudi M, Arsad S, Fitrianesia F, Ramadhan SF, Arif AR, Savitri FR, Dewinta AA, Ongkosongo AD, Musa M, Lusiana ED. 2023. Microalgae diversity in varying habitat characteristics in Pasuruan and Sidoarjo coastal areas, East Java, Indonesia. Biodiversitas 24: 4418-4426. Microalgae are microscopic eukaryotic organisms which live in various types of habitats. The purpose of this study was to identify the types and the abundance of microalgae in several sub-habitats, namely sediments, mangroves, water columns, and artificial substrates; and to analyze the environmental factors that affect the abundance of microalgae. The research was conducted in several sites in the coastal areas in Pasuruan and Sidoarjo, East Java, Indonesia. A quantitative descriptive method was applied with data collection used a purposive sampling method. Samples were collected using net for planktonic microalgae and using sampling plot for epiphytic and benthic microalgae. The microalgae were grouped using NMDS (non-metric multidimensional scaling) and the relationships between microalgae abundance and water quality parameters were analyzed using CCA (canonical correspondence analysis). The results show that Bacillariophyceae, Cyanophyceae, and Chlorophyceae classes were found in all study sites, but Trebouxiophyceae and Dinophyceae were only found in Pasuruan Beach. The highest abundance of microalgae was found in sediment habitat in Wughoyo Beach, Sidoarjo with 706,605 ind. cm-2. The diversity, the evenness, and the dominance index in both coastal areas ranged from 1.43-2.61; 0.71-0.96; and 0.06-0.27, respectively. Similarity analysis using the NMDS showed that there was no similarity among the three sites, suggesting each site had high microalgal variation. The CCA analysis showed that Bacillariophyceae were found in all sites, indicating its high adaptability. The results of this analysis imply that particular habitat has a unique microalgae diversity, thus preserving diverse habitat types is important.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134948459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract. Atikah TA, Purwanti EW. 2023. The effect of peat soil and sandy soil on the growth of Eleutherine palmifolia and arbuscular mycorrhizal diversity. Biodiversitas 24: 4373-4381. Arbuscular mycorrhizae as root symbionts is capable of inducing plant growth on marginal lands. It has the potential to be used as fertilizer or soil enhancer. Mycorrhizae are commonly found in peat soils endemic to the island of Borneo. Peat soil contains a lot of organic matter needed by mycorrhizae. The development of plant roots also influences the process of mycorrhizal colonization. Apart from functioning as fertilizer, mycorrhizae is associated with Dayak shallot (Eleutherine palmifolia Merr.; syn.: Sisyrinchium palmifolium L.) roots can also overcome fusarium wilt disease. This study aimed to explore the potential of peat soil to support the growth of E. palmifolia 's and to identify mycorrhizal colonization associated with the plant. Two soil types were used for planting E. palmifolia: peat and sand. Parameters observed were plant height, number of leaves, morphospecies and each population of arbuscular mycorrhizae. The plant growth data were tabulated and analyzed with an analysis of variance, and the population of arbuscular mycorrhizae was analyzed for the level of similarity in the structure of species. The results showed that peat soil promoted the growth of E. palmifolia better than sandy soil. The similarity value of mycorrhizal species structure was 28.7%. It means that the structure of mycorrhizal species on peat were differed that on sand media. Mycorrhizae successfully explored from sandy soil were 10 morpho-species with a population of 1.441 spores, while mycorrhizae from peat soil contained 6 morphospecies with a population of 462 spores.
摘要Atikah TA, purwananti EW。2023. 泥炭土和沙土对掌叶绿生长及丛枝菌根多样性的影响。生物多样性24:4373-4381。丛枝菌根作为根共生体在边缘土地上具有诱导植物生长的作用。它具有用作肥料或土壤增强剂的潜力。菌根常见于婆罗洲岛特有的泥炭土中。泥炭土含有大量菌根所需的有机质。植物根系的发育也影响菌根定植的过程。菌根除了作为肥料外,还与大葱(Eleutherine palmifolia Merr.;【同上】:西葫芦(Sisyrinchium palmifolium L.)的根也能克服枯萎病。本研究旨在探索泥炭土支持棕榈叶生长的潜力,并确定与该植物相关的菌根定植。采用泥炭和沙土两种土壤类型种植棕榈叶。观测参数为株高、叶数、形态种和丛枝菌根各居群。将植物生长数据制成表格,进行方差分析,并对丛枝菌根种群进行物种结构相似性分析。结果表明,泥炭土对棕榈叶生长的促进作用优于砂质土。菌根菌种结构相似性值为28.7%。说明泥炭培养基上菌根菌种的结构与沙土培养基上不同。从沙质土壤中成功发现的菌根有10个形态种,孢子数量为1.441个;从泥炭土壤中成功发现的菌根有6个形态种,孢子数量为462个。
{"title":"The effect of peat soil and sandy soil on the growth of Eleutherine palmifolia and arbuscular mycorrhizal diversity","authors":"TITIN APUNG ATIKAH, ENY WAHYUNING PURWANTI","doi":"10.13057/biodiv/d240818","DOIUrl":"https://doi.org/10.13057/biodiv/d240818","url":null,"abstract":"Abstract. Atikah TA, Purwanti EW. 2023. The effect of peat soil and sandy soil on the growth of Eleutherine palmifolia and arbuscular mycorrhizal diversity. Biodiversitas 24: 4373-4381. Arbuscular mycorrhizae as root symbionts is capable of inducing plant growth on marginal lands. It has the potential to be used as fertilizer or soil enhancer. Mycorrhizae are commonly found in peat soils endemic to the island of Borneo. Peat soil contains a lot of organic matter needed by mycorrhizae. The development of plant roots also influences the process of mycorrhizal colonization. Apart from functioning as fertilizer, mycorrhizae is associated with Dayak shallot (Eleutherine palmifolia Merr.; syn.: Sisyrinchium palmifolium L.) roots can also overcome fusarium wilt disease. This study aimed to explore the potential of peat soil to support the growth of E. palmifolia 's and to identify mycorrhizal colonization associated with the plant. Two soil types were used for planting E. palmifolia: peat and sand. Parameters observed were plant height, number of leaves, morphospecies and each population of arbuscular mycorrhizae. The plant growth data were tabulated and analyzed with an analysis of variance, and the population of arbuscular mycorrhizae was analyzed for the level of similarity in the structure of species. The results showed that peat soil promoted the growth of E. palmifolia better than sandy soil. The similarity value of mycorrhizal species structure was 28.7%. It means that the structure of mycorrhizal species on peat were differed that on sand media. Mycorrhizae successfully explored from sandy soil were 10 morpho-species with a population of 1.441 spores, while mycorrhizae from peat soil contained 6 morphospecies with a population of 462 spores.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134948626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract. Nuralieva U, Tajiyev K, Sheralieva Z, Toishimanov M, Moldakhmetova G, Temirbayeva K, Tajieva A. 2023. Geometric morphometric characteristics of Apis mellifera honeybee in Kazakhstan. Biodiversitas 24: 4402-4409. This study was conducted in the whole beekeeping region of Kazakhstan. We researched morphological studies using (45 colony bees in 5 main regions in various geographical and climatic zone environmental conditions. The obtained data were compared with the Apis mellifera carnica reference samples, Apis mellifera caucasica, and Apis mellifera mellifera. Morphometric identification was carried out using 19 landmarks by IdentiFly software. Almost all colonies showed belonging to the lineage C (about 85%), by subspecies to A. m. carnica, A. m. caucasica showed 11%, A. m. mellifera belonged to only 4%. Furthermore, using multivariate principal component analysis compared honeybee lineages between reference samples. There were significant differences in wing landmarks between lineages and subspecies (Mahalanobis square distance). By Mahalanobis, distances between the evolutionary lineages M differ from line C by 15.93 and from line O by 19.03 units. In contrast, the distance between lines C and O is insignificant, with satisfactory reliability. The forewing wing size evaluated centroid size, which showed the highest degree of honeybees in the west and south Kazakhstan zones. The percentage of variation in wing centroids was relatively small between analyzed samples (less than 1%) but extremely changed for reference samples A. m. carnica, A. m. caucasica, A. m. mellifera, where log centroid sizes showed 1.33%, 4.18%, and 4.67%, respectively. The results showed significant differences between populations in different climatic zones.
{"title":"Geometric morphometric characteristics of Apis mellifera honeybee in Kazakhstan","authors":"ULZHAN NURALIEVA, KADYRBAI TAJIYEV, ZHANAR SHERALIEVA, MAXAT TOISHIMANOV, GAUKHAR MOLDAKHMETOVA, KAMSHAT TEMIRBAYEVA, AIGUL TAJIEVA","doi":"10.13057/biodiv/d240821","DOIUrl":"https://doi.org/10.13057/biodiv/d240821","url":null,"abstract":"Abstract. Nuralieva U, Tajiyev K, Sheralieva Z, Toishimanov M, Moldakhmetova G, Temirbayeva K, Tajieva A. 2023. Geometric morphometric characteristics of Apis mellifera honeybee in Kazakhstan. Biodiversitas 24: 4402-4409. This study was conducted in the whole beekeeping region of Kazakhstan. We researched morphological studies using (45 colony bees in 5 main regions in various geographical and climatic zone environmental conditions. The obtained data were compared with the Apis mellifera carnica reference samples, Apis mellifera caucasica, and Apis mellifera mellifera. Morphometric identification was carried out using 19 landmarks by IdentiFly software. Almost all colonies showed belonging to the lineage C (about 85%), by subspecies to A. m. carnica, A. m. caucasica showed 11%, A. m. mellifera belonged to only 4%. Furthermore, using multivariate principal component analysis compared honeybee lineages between reference samples. There were significant differences in wing landmarks between lineages and subspecies (Mahalanobis square distance). By Mahalanobis, distances between the evolutionary lineages M differ from line C by 15.93 and from line O by 19.03 units. In contrast, the distance between lines C and O is insignificant, with satisfactory reliability. The forewing wing size evaluated centroid size, which showed the highest degree of honeybees in the west and south Kazakhstan zones. The percentage of variation in wing centroids was relatively small between analyzed samples (less than 1%) but extremely changed for reference samples A. m. carnica, A. m. caucasica, A. m. mellifera, where log centroid sizes showed 1.33%, 4.18%, and 4.67%, respectively. The results showed significant differences between populations in different climatic zones.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134948460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
JEFF M. OPEÑA, REYMELYN A. BUMANGLAG, VENZ MAR T. CABANG
Abstract. Opeña JM, Bumanglag RA, Cabang VMT. 2023. Morphological, phytochemical, and molecular profiling of bamboo species (Bambuseae) growing in various ecosystems of Cagayan Province, Luzon, Philippines. Biodiversitas 24: 4342-4358. The characterization of bamboo species posed advantages to the bamboo industry’s development in the Philippines. This study aimed to provide baseline information on the environmental and biological profiles of bamboo species in Cagayan Province, Philippines. Study sites were visited for bamboo morphological characterization using character states. Leaves were collected for qualitative phytochemical screening of 10 secondary metabolites and DNA sequencing using the rbcL plastid gene. The 28 accessions of Bambusa, Dendrocalamus, Dinochloa, Gigantochloa, Guadua, Melocanna, Phyllostachys, Schizostachyum, and Thyrsostachys were characterized and these bamboos were growing in forests, coastal or sparsely vegetated lands, urban, freshwater, grasslands, and agricultural ecosystems. Bamboo species distinctiveness’ was described through identification keys on growth habit, culm internode, nodal structure, young shoot, and flowering incidence. The bamboos contain coumarins, saponins, steroids, and terpenoids, while quinones are absent. DNA sequencing using rbcL was effective in the identification of Bambusa vulgaris species and unknown bamboo at the species level. Dendrocalamus, Guadua, Melocanna, and Schizostachyum species were successfully identified by rbcL at the genus level. Most of the species were identified as Dendrocalamus species with rbcL. The findings provide valuable information on species identification and certification for asexual propagation, genetic conservation, phytochemical extraction, and utilization for health.
{"title":"Morphological, phytochemical, and molecular profiling of bamboo species (Bambuseae) growing in various ecosystems of Cagayan Province, Luzon, Philippines","authors":"JEFF M. OPEÑA, REYMELYN A. BUMANGLAG, VENZ MAR T. CABANG","doi":"10.13057/biodiv/d240816","DOIUrl":"https://doi.org/10.13057/biodiv/d240816","url":null,"abstract":"Abstract. Opeña JM, Bumanglag RA, Cabang VMT. 2023. Morphological, phytochemical, and molecular profiling of bamboo species (Bambuseae) growing in various ecosystems of Cagayan Province, Luzon, Philippines. Biodiversitas 24: 4342-4358. The characterization of bamboo species posed advantages to the bamboo industry’s development in the Philippines. This study aimed to provide baseline information on the environmental and biological profiles of bamboo species in Cagayan Province, Philippines. Study sites were visited for bamboo morphological characterization using character states. Leaves were collected for qualitative phytochemical screening of 10 secondary metabolites and DNA sequencing using the rbcL plastid gene. The 28 accessions of Bambusa, Dendrocalamus, Dinochloa, Gigantochloa, Guadua, Melocanna, Phyllostachys, Schizostachyum, and Thyrsostachys were characterized and these bamboos were growing in forests, coastal or sparsely vegetated lands, urban, freshwater, grasslands, and agricultural ecosystems. Bamboo species distinctiveness’ was described through identification keys on growth habit, culm internode, nodal structure, young shoot, and flowering incidence. The bamboos contain coumarins, saponins, steroids, and terpenoids, while quinones are absent. DNA sequencing using rbcL was effective in the identification of Bambusa vulgaris species and unknown bamboo at the species level. Dendrocalamus, Guadua, Melocanna, and Schizostachyum species were successfully identified by rbcL at the genus level. Most of the species were identified as Dendrocalamus species with rbcL. The findings provide valuable information on species identification and certification for asexual propagation, genetic conservation, phytochemical extraction, and utilization for health.","PeriodicalId":8894,"journal":{"name":"Biodiversitas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134948462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}