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The use of drone to study the nesting behavior of milky stork: Some preliminary observations 用无人机研究白鹳的筑巢行为:一些初步观察
Q2 Agricultural and Biological Sciences Pub Date : 2023-09-08 DOI: 10.13057/biodiv/d240836
ANI MARDIASTUTI, YENI A. MULYANI, ANDRIE AKBAR, BASUKI WICAKSONO, TIARA RAHMAWATI, YULIA P. WULANDARI, WARSAJAYA WARSAJAYA
Abstract. Mardiastuti A, Mulyani YA, Akbar A, Wicaksono B, Rahmawati T, Wulandari YP, Warsajaya. 2023. The use of drone to study the nesting behavior of milky stork: Some preliminary observations. Biodiversitas 24: 4549-4557. The use of a drone to census waterbirds has been increasingly popular. However, study on the waterbird’s behavioral response to the drone was still limited. The objective of this study is to reveal the response of the breeding milky stork Mycteria cinerea to the drone as a new and safer tool for collecting breeding behavior data. Factors that influence the success of the observation by using a drone were also identified and a set of recommendations was formulated. The research was conducted during the breeding season of the milky stork in Pulau Rambut, a small island in Jakarta Bay, between December 2022 and March 2023. Three types of drones were tested in the pilot study, and eventually, a small manually piloted drone (Syma X25 Pro) was found to be more appropriate for behavioral study. The bird response to the drone (no, neutral, temporary, negative response) was observed through a combination of various distances and angles of the drone to the nest trees. Despite the weather obstacles, this study suggested that the milky stork mostly (17 out of 20 trials) showed no response or neutral response to the drone. Some temporary responses were detected due to some accidental events, including a sudden launch of the drone. In conclusion, a small-size, easy-to-maneuver drone that produces low noise and mild wind can be used by using a manual pilot mode to study the breeding behavior of milky storks or other similar waterbird species.
摘要马迪阿斯图提A, Mulyani YA, Akbar A, Wicaksono B, Rahmawati T, Wulandari YP, Warsajaya. 2023。用无人机研究白鹳的筑巢行为:一些初步观察。生物多样性,24:4549-4557。使用无人机对水鸟进行普查越来越受欢迎。然而,对水鸟对无人机的行为反应的研究仍然有限。本研究的目的是揭示作为一种新的、更安全的采集育种行为数据工具的无人机对繁殖中的白鹳灰分枝杆菌的反应。还确定了影响使用无人机观测成功的因素,并制定了一套建议。这项研究是在2022年12月至2023年3月期间,在雅加达湾的一个小岛普兰布特岛(Pulau Rambut)的白鹳繁殖季节进行的。在初步研究中测试了三种类型的无人机,最终发现小型手动驾驶无人机(Syma X25 Pro)更适合用于行为研究。鸟类对无人机的反应(无,中性,临时,消极的反应)通过无人机到巢树的不同距离和角度的组合来观察。尽管有天气障碍,这项研究表明,大多数乳鹤(20次试验中的17次)对无人机没有反应或中性反应。由于一些意外事件,包括无人机的突然发射,检测到一些临时反应。综上所述,可以使用一种小型、易于操作、噪音低、风力温和的无人机,通过手动驾驶模式来研究乳鹤或其他类似水鸟物种的繁殖行为。
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引用次数: 0
Bioremediation potential of rhizosphere bacterial consortium in lead (Pb) contaminated rice plants 铅污染水稻根际细菌联合体的生物修复潜力
Q2 Agricultural and Biological Sciences Pub Date : 2023-09-05 DOI: 10.13057/biodiv/d240838
MOHAMMAD UBAIDILLAH, NURHALIZA THAMRIN, FADIA INTAN CAHYANI, DINA FITRIYAH
Abstract. Ubaidillah M, Thamrin N, Cahyani FI, Fitriyah D. 2023. Bioremediation potential of rhizosphere bacterial consortium in lead (Pb) contaminated rice plants. Biodiversitas 24: 4566-4571. Lead (Pb) is one of the toxic heavy metals detected in various chemical fertilizers and pesticides used in Indonesia. These chemicals can increase plant lead (Pb) accumulation and affect other organisms through its circulation with food chain. Excess amounts of Pb in soil may be remediated using different approaches such as bioremediation. Rhizosphere bacteria can be used as bioremediation agents to reduce Pb accumulation in plants and also increase plant growth. Actinomycetes, Azotobacter sp., Azospirillum sp., Rhizobium sp., Pseudomonas sp., Lactobacillus sp., Bacillus sp., and Streptomyces sp. are some of the several bacteria that can reduce lead (Pb) accumulation in plants by the ability to sequester Pb, change its oxidation state, or enable Pb to precipitate. This research was conducted to determine the ability of the rhizosphere bacterial consortium to prevent lead (Pb) accumulation in rice plants. This study confirmed that the application of rhizosphere bacterial consortium to rice plants contaminated with lead (Pb) can reduce the plant's lead (Pb) content by up to 45%. The use of a bacterial consortium did not significantly affect the growth parameters of the plant but increased root length by 41%, fresh weight by 32%, dry weight by 26%, and chlorophyll content by 25%, in rice plants contaminated with Pb.
摘要刘建军,刘建军,李建军,等。铅污染水稻根际细菌联合体的生物修复潜力生物多样性24:4566-4571。铅(Pb)是在印度尼西亚使用的各种化肥和农药中检测到的有毒重金属之一。这些化学物质会增加植物铅的积累,并通过其与食物链的循环影响其他生物。土壤中过量的铅可以用不同的方法修复,如生物修复。根际细菌可以作为生物修复剂,减少植物体内的铅积累,促进植物生长。放线菌、固氮菌、固氮螺旋菌、根瘤菌、假单胞菌、乳杆菌、芽孢杆菌和链霉菌是几种可以通过隔离铅、改变其氧化状态或使铅沉淀来减少植物中铅积累的细菌。本研究旨在探讨根际细菌联合体对水稻植株铅积累的抑制作用。本研究证实,在受铅污染的水稻植株上施用根际细菌联合体可使植株的铅含量降低高达45%。利用菌群对铅污染水稻植株的生长参数影响不显著,但使根系长增加41%,鲜重增加32%,干重增加26%,叶绿素含量增加25%。
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引用次数: 0
Chemical characterization of mint (Mentha spp.) germplasm from Central Java, Indonesia 印度尼西亚中爪哇薄荷(薄荷属)种质的化学特性
Q2 Agricultural and Biological Sciences Pub Date : 2023-09-05 DOI: 10.13057/biodiv/d240812
LIANAH LIANAH, NIKEN KUSUMARINI, MUTISTA HAFSHAH, KRISANTINI KRISANTINI, ANI KURNIAWATI, MILYA URFA AHMAD
Abstract. Lianah L, Kusumarini N, Hafshah M, Krisantini K, Kurniawati A, Ahmad MA. 2023. Chemical characterization of mint (Mentha spp.) germplasm from Central Java, Indonesia. Biodiversitas 24: 4307-4313. The main objective of this work was to evaluate and compare the leaf production and oil content of the four mint genotypes (Mentha spp.) from Central Java, Indonesia. Four genotypes were collected from Rejosari Village, Kudus District, Central Java, Indonesia, namely spearmint (Mentha spicata), peppermint (Mentha x piperita), orange mint (Mentha x piperita ‘Orange’) and chocolate mint (Mentha x piperita ‘Chocolate’). Essential oil extraction was carried out by steam distillation. Identification of the chemical compounds of the oil and their relative percentage were analyzed using GC-MS. Leaves productivity ranged between 500-1000 kg/ha, and essential oil content from 0.4 to 1.36%. The major essential oil constituents detected were 1,8-cineole, carvone, limonene, linalool, linalyl acetate, menthol, menthone, menthyl acetate, and piperitenone oxide. Chocolate mint had the highest leaf production and the highest content of essential oil (4.17%) and menthone yield (28%) compared to the other genotypes.
摘要Lianah L, Kusumarini N, Hafshah M, Krisantini K, Kurniawati A, Ahmad MA。2023. 印度尼西亚中爪哇薄荷(薄荷属)种质的化学特性。生物多样性24:4307-4313。本研究的主要目的是评价和比较来自印度尼西亚中爪哇的四种薄荷基因型(薄荷属)的叶片产量和含油量。从印度尼西亚中爪哇省Kudus地区的Rejosari村收集了4种基因型,即绿薄荷(薄荷)、薄荷(薄荷x胡椒)、橙薄荷(薄荷x胡椒'橙')和巧克力薄荷(薄荷x胡椒'巧克力')。采用水蒸气蒸馏法提取精油。采用气相色谱-质谱联用技术对其化学成分进行了鉴定,并对其相对含量进行了分析。叶片产量在500-1000公斤/公顷之间,精油含量在0.4 - 1.36%之间。主要精油成分为1,8-桉树脑、香芹酮、柠檬烯、芳樟醇、乙酸芳樟醇、薄荷醇、薄荷酮、乙酸薄荷酯和氧化胡椒烯酮。与其他基因型相比,巧克力薄荷的叶片产量最高,精油含量最高(4.17%),薄荷酮产量最高(28%)。
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引用次数: 0
Distribution of Arbuscular Mycorrhiza Fungi (AM Fungi) in different plants within tailings deposition areas of Freeport Indonesia, Timika, Central Papua, Indonesia 丛枝菌根真菌(AM真菌)在印度尼西亚自由港尾矿堆积区不同植物中的分布
Q2 Agricultural and Biological Sciences Pub Date : 2023-09-05 DOI: 10.13057/biodiv/d240832
IRNANDA AIKO FIFI DJUUNA, NUNANG LAMAEK MAY, SITI HAJAR KUBANGUN, MARIA MASSORA, SOBA WILLEM AIBINI
Abstract. Djuuna IAF, May NL, Kubangun SH, Massora M, Aibini SW. 2023. Distribution of Arbuscular Mycorrhiza Fungi (AM Fungi) in different plants within tailings deposition areas of Freeport Indonesia, Timika, Central Papua, Indonesia. Biodiversitas 24: 4515-4522. This study investigated the presence of Arbuscular Mycorrhiza Fungi (AM Fungi) in various vegetation types within tailings deposition areas in Freeport Timika, Papua. Soil and root samples were collected from 41 sampling locations, representing 28 types of vegetation growing in these areas. AM Fungi spores were extracted using the wet sieving technique, while root samples were cleaned, stained, and analyzed for the percentage of root infection using the gridlines method. Some soil characteristics were also analyzed, including pH (H2O), moisture content, organic carbon (C), Total nitrogen (N) and phosphorus (P), and soil texture. The study showed low total soil N and P levels, with organic C ranging from low to high and a pH range from 6 to 6.7. The spore number ranged from 2 to 20 spores/10 g soil, and the percentage of root colonization varied from 17 to 89.5%. The highest number of spores (20 spores/10 g soil) was found in the rhizosphere of Homalanthus sp. and Polyalthia glauca, while the highest percentage of root colonization (89.5%) was observed under Pandanus sp. at Upper ADA. Notably, the highest number of spores did not correspond to the highest percentage of infected roots. Additionally, four genera of AM Fungi, namely Glomus, Acaulospora, Scuttelospora and Gigaspora, were found to thrive in tailings areas and have the potential to be developed as biofertilizers for tailings rehabilitation programs.
摘要Djuuna IAF, May NL, Kubangun SH, Massora M, Aibini SW。2023. 丛枝菌根真菌(AM真菌)在印度尼西亚自由港尾矿堆积区不同植物中的分布。生物多样性24:4515-4522。本研究调查了在巴布亚自由港Timika尾矿堆放区的各种植被类型中存在的丛枝菌根真菌(AM真菌)。从41个采样点收集了土壤和根系样本,代表了这些地区生长的28种植被。采用湿筛技术提取AM真菌孢子,同时对根样品进行清洗、染色,并使用网格线法分析根感染百分比。分析了土壤的pH (H2O)、含水量、有机碳(C)、全氮(N)、全磷(P)和土壤质地等特征。研究表明,土壤全氮、全磷水平较低,有机碳由低到高,pH值从6到6.7。孢子数为2 ~ 20个/10 g土壤,根定植率为17% ~ 89.5%。在Upper ADA, Homalanthus sp.和Polyalthia glauca的根际孢子数最高(20个孢子/10 g土壤),而Pandanus sp.的根际定植率最高(89.5%)。值得注意的是,孢子数量最多并不对应于感染根的最高百分比。此外,发现AM真菌Glomus、Acaulospora、stcuttelospora和Gigaspora 4属在尾矿区大量繁殖,具有开发作为尾矿修复工程生物肥料的潜力。
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引用次数: 0
In vitro characterization of UB Forest (Malang, Indonesia) indigenous bacteria as plant growth promoting bacteria (PGPB) UB森林(Malang, Indonesia)原生细菌作为植物生长促进菌(PGPB)的体外鉴定
Q2 Agricultural and Biological Sciences Pub Date : 2023-09-04 DOI: 10.13057/biodiv/d240837
LUQMAN QURATA AINI, IRISA TRIANTI, SHOLIKAH WIDYANITTA RACHMAWATI, ANTON MEILUS PUTRA, NABILLA ALYA ANASTASYA, ADI SETIAWAN
Abstract. Aini LQ, Trianti I, Rachmawati SW, Putra AM, Anastasya NA, Setiawan A. 2023. In vitro characterization of UB Forest (Malang, Indonesia) indigenous bacteria as plant growth promoting bacteria (PGPB). Biodiversitas 24: 4558-4565. UB Forest, an educational forest of Universitas Brawijaya in Malang, East Java, has a mega-biodiversity of microbial germplasm. Previously, we obtained several UB Forest indigenous bacterial isolates and tested them on various plant commodities. However, the comprehensive characterization of the bacteria to produce IAA hormone, to overcome biotic and abiotic stresses as well as their potency as biofertilizers in vitro has not been carried out yet. In this study, we conducted in vitro assays to elucidate the potency of UB Forest indigenous bacterial isolates as Plant Growth Promoting Bacteria (PGPB). The molecular identification showed that the bacterial strains were dominated by the Pseudomonads, namely Pseudomonas versuta UB-36, P. aeruginosa UB-52, P. lundensis UB-53, P. migulae UB-54, and P. koreensis UB-62. Other strains were Enterococcus gallinarum UB-55 and Lysinibacillus fusiformis UB-64. Of the 7 bacterial strains, only 3 could inhibit Xanthomonas campestris. All bacterial strains were able to produce IAA, whereas five bacteria can solubilize phosphate, six bacteria can fix nitrogen, and four bacteria have both activities. All bacterial strains can grow at pH 5-6, salinity 5-15%, temperature 60°C, and 15% polyethylene glycol (PEG) drought stress media. The results suggested that the UB Forest indigenous bacterial strains have a role as plant growth-promoting bacteria (PGPB) and are expected to support the growth of plants grown under biotic and abiotic stress conditions.
摘要Aini LQ, Trianti I, Rachmawati SW, Putra AM, Anastasya NA, Setiawan A. 2023。UB森林(Malang, Indonesia)原生细菌作为植物生长促进菌(PGPB)的体外鉴定生物多样性,24:4558-4565。UB森林是东爪哇玛琅的布拉维加亚大学的一个教育森林,拥有丰富的微生物种质资源。以前,我们获得了几个UB森林本地细菌分离株,并在各种植物商品上进行了测试。然而,目前还没有对产生IAA激素、克服生物和非生物胁迫的细菌及其作为生物肥料的体外效能进行全面的表征。在这项研究中,我们进行了体外实验来阐明UB森林本土细菌分离物作为植物生长促进细菌(PGPB)的效力。分子鉴定结果表明,菌株以假单胞菌为主,分别为反假单胞菌UB-36、铜绿假单胞菌UB-52、隆德假单胞菌UB-53、乳沟假单胞菌UB-54和韩国假单胞菌UB-62。其他菌株为鸡肠球菌UB-55和梭状芽胞杆菌UB-64。7株细菌中,仅有3株能抑制油菜黄单胞菌。所有菌株都能产生IAA,其中5株细菌能溶解磷酸盐,6株细菌能固定氮,4株细菌同时具有这两种活性。所有菌株均能在pH 5-6、盐度5-15%、温度60℃和15%聚乙二醇(PEG)干旱胁迫介质下生长。结果表明,UB森林本地菌株具有植物生长促进菌(PGPB)的作用,有望在生物和非生物胁迫条件下支持植物的生长。
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引用次数: 0
Probiotics potential of lactic acid bacteria isolated from Slender Walking Catfish (Clarias nieuhofii) 细长鲇鱼乳酸菌的益生菌潜力研究
Q2 Agricultural and Biological Sciences Pub Date : 2023-09-04 DOI: 10.13057/biodiv/d240839
RAHMAD LINGGA, SUDIRMAN ADIBRATA, OCCA ROANISCA, SIPRIYADI SIPRIYADI, RISKY HADI WIBOWO, AHMAD ARSYADI
Abstract. Lingga R, Adibrata S, Roanisca O, Sipriyadi, Wibowo RH, Arsyadi. 2023. Probiotics potential of lactic acid bacteria isolated from Slender Walking Catfish (Clarias nieuhofii). Biodiversitas 24: 4572-4580. Probiotics are living microorganisms or bioactive agents that positively impact animal digestion. They have successfully isolated from various sources. Recently, we isolated and characterized lactic acid bacteria (LAB) from slender walking fish (Clarias nieuhofii Valenciennes, 1840). The fish sample was obtained from Batu Rusa and Paya Benua Rivers in Bangka Island, Indonesia. LAB was isolated from the fish intestine using the pour plate method. The isolated LAB was then characterized based on their phenotypic traits, biochemical properties, and the 16S rRNA gene identification. The chosen isolates were tested to determine their ability to produce lactic acid, hemolytic and antibacterial activity, and antibiotic resistance. All isolates have the characteristic of rod-shaped and short rod-shaped cells with Gram-positive properties. Isolate KP1 showed the number of populations (2.89 x 107 CFU/mL) and lactic acid production with a concentration of 1.85%. All isolates exhibited no hemolysis activity and displayed sensitivity to antibiotics. The twelve lactic acid bacteria formed clear zones against Staphylococcus aureus and Escherichia coli. The results of the 16S rRNA gene identification indicated that isolates KB4, KB7, KB8, and KP1 belong to Lactobacillus vaginalis, Lactobacillus fermentum, Limosilactobacillus fermentum, and Levilactobacillus brevis, respectively. Lactic acid bacteria isolated from slender walking fish exhibited potential probiotic traits.
摘要林嘉R,阿蒂布拉塔S,罗安尼斯卡O, Sipriyadi, Wibowo RH, Arsyadi. 2023。细长鲇鱼乳酸菌的益生菌潜力研究生物多样性,24:4572-4580。益生菌是对动物消化有积极影响的活微生物或生物活性物质。他们已成功地与各种来源隔绝。最近,我们从细长的行走鱼(Clarias nieuhofii Valenciennes, 1840)中分离并鉴定了乳酸菌(LAB)。鱼类样本取自印度尼西亚邦加岛的Batu Rusa河和Paya Benua河。采用倾板法从鱼肠中分离乳酸菌。然后根据其表型性状、生化特性和16S rRNA基因鉴定对分离的LAB进行表征。对所选菌株进行了测试,以确定其产生乳酸、溶血和抗菌活性以及抗生素耐药性的能力。所有分离株均具有革兰氏阳性的杆状和短杆状细胞的特征。分离株KP1的菌群数为2.89 × 107 CFU/mL,乳酸产量为1.85%。所有分离株均无溶血活性,对抗生素敏感。12种乳酸菌对金黄色葡萄球菌和大肠杆菌形成了清晰的区域。16S rRNA基因鉴定结果表明,分离株KB4、KB7、KB8和KP1分别属于阴道乳杆菌、发酵乳杆菌、发酵乳杆菌和短Levilactobacillus brevis。从细长行走鱼中分离的乳酸菌表现出潜在的益生菌特性。
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引用次数: 0
Microalgae diversity in varying habitat characteristics in Pasuruan and Sidoarjo coastal areas, East Java, Indonesia 印度尼西亚东爪哇Pasuruan和Sidoarjo沿海地区不同生境特征的微藻多样性
Q2 Agricultural and Biological Sciences Pub Date : 2023-09-03 DOI: 10.13057/biodiv/d240823
MOHAMMAD MAHMUDI, SULASTRI ARSAD, FIKA FITRIANESIA, SAFRIZAL FIKRI RAMADHAN, ABDUR RAHMAN ARIF, FRIZCA RANIA SAVITRI, ADELITA ASMO DEWINTA, ANINDYA DIVANATA ONGKOSONGO, MUHAMMAD MUSA, EVELLIN DEWI LUSIANA
Abstract. Mahmudi M, Arsad S, Fitrianesia F, Ramadhan SF, Arif AR, Savitri FR, Dewinta AA, Ongkosongo AD, Musa M, Lusiana ED. 2023. Microalgae diversity in varying habitat characteristics in Pasuruan and Sidoarjo coastal areas, East Java, Indonesia. Biodiversitas 24: 4418-4426. Microalgae are microscopic eukaryotic organisms which live in various types of habitats. The purpose of this study was to identify the types and the abundance of microalgae in several sub-habitats, namely sediments, mangroves, water columns, and artificial substrates; and to analyze the environmental factors that affect the abundance of microalgae. The research was conducted in several sites in the coastal areas in Pasuruan and Sidoarjo, East Java, Indonesia. A quantitative descriptive method was applied with data collection used a purposive sampling method. Samples were collected using net for planktonic microalgae and using sampling plot for epiphytic and benthic microalgae. The microalgae were grouped using NMDS (non-metric multidimensional scaling) and the relationships between microalgae abundance and water quality parameters were analyzed using CCA (canonical correspondence analysis). The results show that Bacillariophyceae, Cyanophyceae, and Chlorophyceae classes were found in all study sites, but Trebouxiophyceae and Dinophyceae were only found in Pasuruan Beach. The highest abundance of microalgae was found in sediment habitat in Wughoyo Beach, Sidoarjo with 706,605 ind. cm-2. The diversity, the evenness, and the dominance index in both coastal areas ranged from 1.43-2.61; 0.71-0.96; and 0.06-0.27, respectively. Similarity analysis using the NMDS showed that there was no similarity among the three sites, suggesting each site had high microalgal variation. The CCA analysis showed that Bacillariophyceae were found in all sites, indicating its high adaptability. The results of this analysis imply that particular habitat has a unique microalgae diversity, thus preserving diverse habitat types is important.
摘要Mahmudi M, Arsad S, Fitrianesia F, Ramadhan SF, Arif AR, Savitri FR, Dewinta AA, Ongkosongo AD, Musa M, luusiana ED. 2023。印度尼西亚东爪哇Pasuruan和Sidoarjo沿海地区不同生境特征的微藻多样性。生物多样性24:4418-4426。微藻是一种微小的真核生物,生活在各种类型的栖息地。本研究的目的是确定沉积物、红树林、水柱和人工基质中微藻的类型和丰度;并分析影响微藻丰度的环境因素。这项研究是在印度尼西亚东爪哇Pasuruan和Sidoarjo沿海地区的几个地点进行的。采用定量描述方法,采用目的抽样法收集数据。浮游微藻采用网捕法采集,附生微藻和底栖微藻采用样地采集。采用非度量多维尺度(NMDS)对微藻进行分组,并采用典型对应分析(CCA)分析微藻丰度与水质参数的关系。结果表明:在所有研究点均发现硅藻纲、蓝藻纲和绿藻纲,但仅在帕苏鲁滩发现海藻纲和藻纲。微藻丰度最高的是Sidoarjo的Wughoyo海滩沉积物栖息地,为706,605 ind.cm -2。多样性、均匀度和优势度指数在1.43 ~ 2.61之间;0.71 - -0.96;和0.06-0.27。NMDS相似性分析显示,3个站点之间没有相似性,说明每个站点的微藻差异较大。CCA分析显示,在所有地点均发现了硅藻门,表明其适应性强。这一分析结果表明,特定的生境具有独特的微藻多样性,因此保护不同的生境类型是重要的。
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引用次数: 0
The effect of peat soil and sandy soil on the growth of Eleutherine palmifolia and arbuscular mycorrhizal diversity 泥炭土和沙土对掌叶绿生长及丛枝菌根多样性的影响
Q2 Agricultural and Biological Sciences Pub Date : 2023-09-03 DOI: 10.13057/biodiv/d240818
TITIN APUNG ATIKAH, ENY WAHYUNING PURWANTI
Abstract. Atikah TA, Purwanti EW. 2023. The effect of peat soil and sandy soil on the growth of Eleutherine palmifolia and arbuscular mycorrhizal diversity. Biodiversitas 24: 4373-4381. Arbuscular mycorrhizae as root symbionts is capable of inducing plant growth on marginal lands. It has the potential to be used as fertilizer or soil enhancer. Mycorrhizae are commonly found in peat soils endemic to the island of Borneo. Peat soil contains a lot of organic matter needed by mycorrhizae. The development of plant roots also influences the process of mycorrhizal colonization. Apart from functioning as fertilizer, mycorrhizae is associated with Dayak shallot (Eleutherine palmifolia Merr.; syn.: Sisyrinchium palmifolium L.) roots can also overcome fusarium wilt disease. This study aimed to explore the potential of peat soil to support the growth of E. palmifolia 's and to identify mycorrhizal colonization associated with the plant. Two soil types were used for planting E. palmifolia: peat and sand. Parameters observed were plant height, number of leaves, morphospecies and each population of arbuscular mycorrhizae. The plant growth data were tabulated and analyzed with an analysis of variance, and the population of arbuscular mycorrhizae was analyzed for the level of similarity in the structure of species. The results showed that peat soil promoted the growth of E. palmifolia better than sandy soil. The similarity value of mycorrhizal species structure was 28.7%. It means that the structure of mycorrhizal species on peat were differed that on sand media. Mycorrhizae successfully explored from sandy soil were 10 morpho-species with a population of 1.441 spores, while mycorrhizae from peat soil contained 6 morphospecies with a population of 462 spores.
摘要Atikah TA, purwananti EW。2023. 泥炭土和沙土对掌叶绿生长及丛枝菌根多样性的影响。生物多样性24:4373-4381。丛枝菌根作为根共生体在边缘土地上具有诱导植物生长的作用。它具有用作肥料或土壤增强剂的潜力。菌根常见于婆罗洲岛特有的泥炭土中。泥炭土含有大量菌根所需的有机质。植物根系的发育也影响菌根定植的过程。菌根除了作为肥料外,还与大葱(Eleutherine palmifolia Merr.;【同上】:西葫芦(Sisyrinchium palmifolium L.)的根也能克服枯萎病。本研究旨在探索泥炭土支持棕榈叶生长的潜力,并确定与该植物相关的菌根定植。采用泥炭和沙土两种土壤类型种植棕榈叶。观测参数为株高、叶数、形态种和丛枝菌根各居群。将植物生长数据制成表格,进行方差分析,并对丛枝菌根种群进行物种结构相似性分析。结果表明,泥炭土对棕榈叶生长的促进作用优于砂质土。菌根菌种结构相似性值为28.7%。说明泥炭培养基上菌根菌种的结构与沙土培养基上不同。从沙质土壤中成功发现的菌根有10个形态种,孢子数量为1.441个;从泥炭土壤中成功发现的菌根有6个形态种,孢子数量为462个。
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引用次数: 0
Geometric morphometric characteristics of Apis mellifera honeybee in Kazakhstan 哈萨克斯坦蜜蜂几何形态计量学特征
Q2 Agricultural and Biological Sciences Pub Date : 2023-09-03 DOI: 10.13057/biodiv/d240821
ULZHAN NURALIEVA, KADYRBAI TAJIYEV, ZHANAR SHERALIEVA, MAXAT TOISHIMANOV, GAUKHAR MOLDAKHMETOVA, KAMSHAT TEMIRBAYEVA, AIGUL TAJIEVA
Abstract. Nuralieva U, Tajiyev K, Sheralieva Z, Toishimanov M, Moldakhmetova G, Temirbayeva K, Tajieva A. 2023. Geometric morphometric characteristics of Apis mellifera honeybee in Kazakhstan. Biodiversitas 24: 4402-4409. This study was conducted in the whole beekeeping region of Kazakhstan. We researched morphological studies using (45 colony bees in 5 main regions in various geographical and climatic zone environmental conditions. The obtained data were compared with the Apis mellifera carnica reference samples, Apis mellifera caucasica, and Apis mellifera mellifera. Morphometric identification was carried out using 19 landmarks by IdentiFly software. Almost all colonies showed belonging to the lineage C (about 85%), by subspecies to A. m. carnica, A. m. caucasica showed 11%, A. m. mellifera belonged to only 4%. Furthermore, using multivariate principal component analysis compared honeybee lineages between reference samples. There were significant differences in wing landmarks between lineages and subspecies (Mahalanobis square distance). By Mahalanobis, distances between the evolutionary lineages M differ from line C by 15.93 and from line O by 19.03 units. In contrast, the distance between lines C and O is insignificant, with satisfactory reliability. The forewing wing size evaluated centroid size, which showed the highest degree of honeybees in the west and south Kazakhstan zones. The percentage of variation in wing centroids was relatively small between analyzed samples (less than 1%) but extremely changed for reference samples A. m. carnica, A. m. caucasica, A. m. mellifera, where log centroid sizes showed 1.33%, 4.18%, and 4.67%, respectively. The results showed significant differences between populations in different climatic zones.
摘要刘建军,张建军,张建军,张建军,张建军,张建军。哈萨克斯坦蜜蜂几何形态计量学特征。生物多样性24:4402-4409。本研究在哈萨克斯坦整个养蜂区进行。在不同的地理和气候带环境条件下,对5个主要地区的45只蜂群进行了形态学研究。并将所得数据与北美蜜蜂参考样本、高加索蜜蜂参考样本和中国蜜蜂参考样本进行比较。利用IdentiFly软件对19个地标进行形态计量鉴定。几乎所有的群落都属于C系(约85%),按亚种划分为尼卡a.m.b arnica,高加索a.m.b caucasica占11%,蜜蜂a.m.b mellifera仅占4%。此外,利用多元主成分分析比较了参考样本之间的蜜蜂谱系。不同谱系和亚种间(马氏鳞鱼的平方距离)翅膀标志有显著差异。以马氏为例,进化谱系M与C系之间的距离相差15.93个单位,与O系之间的距离相差19.03个单位。相比之下,C线与O线之间的距离不显著,可靠性令人满意。前翅翅大小评价质心大小,表明哈萨克斯坦西部和南部地区的蜜蜂程度最高。翅形质心在分析样本间的变异百分率相对较小(小于1%),但参考样本A. m.b arnica、A. m.b caucasica和A. m.b mellifera的对数质心大小变化极大,分别为1.33%、4.18%和4.67%。结果表明,不同气候带种群间存在显著差异。
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引用次数: 0
Morphological, phytochemical, and molecular profiling of bamboo species (Bambuseae) growing in various ecosystems of Cagayan Province, Luzon, Philippines 菲律宾吕宋岛卡加延省不同生态系统中竹类(竹科)形态、植物化学和分子分析
Q2 Agricultural and Biological Sciences Pub Date : 2023-09-03 DOI: 10.13057/biodiv/d240816
JEFF M. OPEÑA, REYMELYN A. BUMANGLAG, VENZ MAR T. CABANG
Abstract. Opeña JM, Bumanglag RA, Cabang VMT. 2023. Morphological, phytochemical, and molecular profiling of bamboo species (Bambuseae) growing in various ecosystems of Cagayan Province, Luzon, Philippines. Biodiversitas 24: 4342-4358. The characterization of bamboo species posed advantages to the bamboo industry’s development in the Philippines. This study aimed to provide baseline information on the environmental and biological profiles of bamboo species in Cagayan Province, Philippines. Study sites were visited for bamboo morphological characterization using character states. Leaves were collected for qualitative phytochemical screening of 10 secondary metabolites and DNA sequencing using the rbcL plastid gene. The 28 accessions of Bambusa, Dendrocalamus, Dinochloa, Gigantochloa, Guadua, Melocanna, Phyllostachys, Schizostachyum, and Thyrsostachys were characterized and these bamboos were growing in forests, coastal or sparsely vegetated lands, urban, freshwater, grasslands, and agricultural ecosystems. Bamboo species distinctiveness’ was described through identification keys on growth habit, culm internode, nodal structure, young shoot, and flowering incidence. The bamboos contain coumarins, saponins, steroids, and terpenoids, while quinones are absent. DNA sequencing using rbcL was effective in the identification of Bambusa vulgaris species and unknown bamboo at the species level. Dendrocalamus, Guadua, Melocanna, and Schizostachyum species were successfully identified by rbcL at the genus level. Most of the species were identified as Dendrocalamus species with rbcL. The findings provide valuable information on species identification and certification for asexual propagation, genetic conservation, phytochemical extraction, and utilization for health.
摘要Opeña JM, hummanglg RA, Cabang VMT。2023. 菲律宾吕宋岛卡加延省不同生态系统中竹类(竹科)形态、植物化学和分子分析生物多样性24:4342-4358。竹的种类特征为菲律宾竹产业的发展提供了有利条件。本研究旨在为菲律宾卡加延省竹子物种的环境和生物概况提供基线信息。利用竹的性状状态对研究地点进行了形态表征。收集叶片,对10种次生代谢产物进行定性植物化学筛选,并利用rbcL质体基因进行DNA测序。竹科(Bambusa)、竹竹科(Dendrocalamus)、龙竹科(Dinochloa)、巨竹科(Gigantochloa)、瓜多科(Guadua)、Melocanna、毛竹科(Phyllostachys)、裂竹科(Schizostachyum)和水竹科(Thyrsostachys)共28种,主要生长在森林、沿海或疏地、城市、淡水、草原和农业生态系统中。通过生长习性、茎节间、节结构、幼枝和开花率等识别键来描述竹的物种差异性。竹子含有香豆素、皂苷、类固醇和萜类,而不含醌类。利用rbcL进行DNA测序,可以在种水平上对竹种和未知竹进行有效的鉴定。用rbcL在属水平上成功地鉴定了树菖蒲、瓜多亚、Melocanna和Schizostachyum。大多数种经rbcL鉴定为石菖蒲属。这些发现为无性繁殖、遗传保护、植物化学提取和卫生利用提供了有价值的物种鉴定和认证信息。
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引用次数: 0
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