Pub Date : 2025-02-01DOI: 10.1016/j.biteb.2025.102029
Srinithya Ravinuthala, Saravanan Settu
Microbial Fuel Cells (MFCs) can treat wastewater, generating electricity simultaneously. However, major issues for up-scaling MFCs are expensive materials and low power densities. In this study, single-chamber MFCs made of low-cost materials i.e., earthen pot separator, stainless-steel mesh electrodes, cathode catalyst as activated carbon and manganese oxide mixture were used; cost of materials totaling less than a dollar. Using dairy wastewater as substrate, system optimization was done by studying effects of pH, external resistance, and co-substrate using Response Surface Methodology (RSM) for maximization of power density, COD removal percentage and columbic efficiency. Acidic pH, higher external resistance, and acetic acid co-substrate conditions showed optimized results. Cyclic Voltammetry and Electrochemical Impedance Spectroscopy were tested, studying effects of pH on biofilm formation. Consistent biofilm seen in case of neutral pH than pH 6 & pH 8. RSM had proposed quadratic models for all three parameters. Confirmation run carried out showed satisfactory results.
{"title":"Multi-response parametric optimization and biofilm studies of low-cost ceramic microbial fuel cell for dairy wastewater treatment","authors":"Srinithya Ravinuthala, Saravanan Settu","doi":"10.1016/j.biteb.2025.102029","DOIUrl":"10.1016/j.biteb.2025.102029","url":null,"abstract":"<div><div>Microbial Fuel Cells (MFCs) can treat wastewater, generating electricity simultaneously. However, major issues for up-scaling MFCs are expensive materials and low power densities. In this study, single-chamber MFCs made of low-cost materials i.e., earthen pot separator, stainless-steel mesh electrodes, cathode catalyst as activated carbon and manganese oxide mixture were used; cost of materials totaling less than a dollar. Using dairy wastewater as substrate, system optimization was done by studying effects of pH, external resistance, and co-substrate using Response Surface Methodology (RSM) for maximization of power density, COD removal percentage and columbic efficiency. Acidic pH, higher external resistance, and acetic acid co-substrate conditions showed optimized results. Cyclic Voltammetry and Electrochemical Impedance Spectroscopy were tested, studying effects of pH on biofilm formation. Consistent biofilm seen in case of neutral pH than pH 6 & pH 8. RSM had proposed quadratic models for all three parameters. Confirmation run carried out showed satisfactory results.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"29 ","pages":"Article 102029"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.biteb.2025.102024
Jianyong Zhu , Wuxi Chen , Yuxiang Chen , Farrukh Raza Amin , Yaxiang Li , Mengjia Lu , Demao Li
Airlift reactors are widely used in the field of microbial fermentation because of their simple structure and easy scale-up. This study investigated the enhancement of gas–liquid two-phase flow and mass transfer in a laboratory-scale airlift reactor for Fusarium venenatum fermentation through structural modifications. First, CFD modeling of the bubble reactor in the laboratory was performed and the reliability of the CFD model was verified by cold model experiments. Secondly, The addition of a baffle in the original reactor transforms it into an internal-loop split airlift reactor (ALR). The baffle was introduced into the initial reactor, and CFD was employed to predict the optimal installation position of the baffle. The results indicate that when a gap of 60 mm is maintained between the upper edge of the baffle and the liquid surface, a 40 mm gap between the lower edge of the baffle and the bottom of the reactor, and an Ad/Ar ratio of 1, the internal-loop split ALR exhibited a 23.55 % increase in gas holdup and a 30.07 % improvement in kLa compared to the original reactor., and the speed dead zone has been significantly improved. Following these specifications, baffe were installed, and the performance of the modified reactor was assessed against the original design during F. venenatum fermentation. The modified reactor demonstrated a significant enhancement in internal circulation, gas holdup, and mass transfer efficiency. Finally, the modified reactor was used for the fermentation experiment of F. venenatum. The fermentation results of F. venenatum showed that the glucose consumption rate of the modified reactor increased by 0.42 g/L/h, and the biomass increased by 3.49 g/L. The glucose conversion rate increased by 66.7 %, and the protein content increased by 3.69 %. These results showed that the internal-loop split ALR substantially improved the efficiency of the F. venenatum fermentation process.
{"title":"CFD optimization of an air lift fermenter for Fusarium venenatum fermentation","authors":"Jianyong Zhu , Wuxi Chen , Yuxiang Chen , Farrukh Raza Amin , Yaxiang Li , Mengjia Lu , Demao Li","doi":"10.1016/j.biteb.2025.102024","DOIUrl":"10.1016/j.biteb.2025.102024","url":null,"abstract":"<div><div>Airlift reactors are widely used in the field of microbial fermentation because of their simple structure and easy scale-up. This study investigated the enhancement of gas–liquid two-phase flow and mass transfer in a laboratory-scale airlift reactor for <em>Fusarium venenatum</em> fermentation through structural modifications. First, CFD modeling of the bubble reactor in the laboratory was performed and the reliability of the CFD model was verified by cold model experiments. Secondly, The addition of a baffle in the original reactor transforms it into an internal-loop split airlift reactor (ALR). The baffle was introduced into the initial reactor, and CFD was employed to predict the optimal installation position of the baffle. The results indicate that when a gap of 60 mm is maintained between the upper edge of the baffle and the liquid surface, a 40 mm gap between the lower edge of the baffle and the bottom of the reactor, and an A<sub>d</sub>/A<sub>r</sub> ratio of 1, the internal-loop split ALR exhibited a 23.55 % increase in gas holdup and a 30.07 % improvement in <em>k</em><sub><em>L</em></sub><em>a</em> compared to the original reactor., and the speed dead zone has been significantly improved. Following these specifications, baffe were installed, and the performance of the modified reactor was assessed against the original design during <em>F. venenatum</em> fermentation. The modified reactor demonstrated a significant enhancement in internal circulation, gas holdup, and mass transfer efficiency. Finally, the modified reactor was used for the fermentation experiment of <em>F. venenatum</em>. The fermentation results of <em>F. venenatum</em> showed that the glucose consumption rate of the modified reactor increased by 0.42 g/L/h, and the biomass increased by 3.49 g/L. The glucose conversion rate increased by 66.7 %, and the protein content increased by 3.69 %. These results showed that the internal-loop split ALR substantially improved the efficiency of the <em>F. venenatum</em> fermentation process.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"29 ","pages":"Article 102024"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.biteb.2025.102050
Pham Thi Le Na, Nguyen Le The Quang, Nguyen Thi Phuong Thao, Hoang Thi Kim Chi, Bao-Trong Dang
In this study, corncob biomass was pyrolyzed at 400 °C (BC400) and 600 °C (BC600) to investigate the sorption properties of sulfamethoxazole (SMX). Sorption experiments were conducted at different pH levels (3, 7, and 10) and solution temperatures (20, 26, and 32 °C). The results showed that the biochar yield of BC400 (31.68 ± 0.85 %) was higher than that of BC600 (26.10 ± 0.42 %). However, BC600 significantly improved the surface area (279 m2 g−1) compared to BC400 (4.8 m2 g−1), resulting in a threefold increase in SMX0 sorption at pH 3 (5.849 mmol kg−1 vs. 1.803 mmol kg−1). At pH 7, the sorption for SMX− reached 2.859 mmol kg−1 for BC600, while no sorption was observed for BC400. Computational simulations indicated that sorption is spontaneous, endothermic, and primarily entropy-driven, governed by intraparticle diffusion. This work highlights the importance of pyrolysis temperature for enhancing SMX− sorption at neutral pH using pristine corncob biochar.
{"title":"Harnessing corncob-derived materials for the elimination of sulfamethoxazole from water","authors":"Pham Thi Le Na, Nguyen Le The Quang, Nguyen Thi Phuong Thao, Hoang Thi Kim Chi, Bao-Trong Dang","doi":"10.1016/j.biteb.2025.102050","DOIUrl":"10.1016/j.biteb.2025.102050","url":null,"abstract":"<div><div>In this study, corncob biomass was pyrolyzed at 400 °C (BC400) and 600 °C (BC600) to investigate the sorption properties of sulfamethoxazole (SMX). Sorption experiments were conducted at different pH levels (3, 7, and 10) and solution temperatures (20, 26, and 32 °C). The results showed that the biochar yield of BC400 (31.68 ± 0.85 %) was higher than that of BC600 (26.10 ± 0.42 %). However, BC600 significantly improved the surface area (279 m<sup>2</sup> g<sup>−1</sup>) compared to BC400 (4.8 m<sup>2</sup> g<sup>−1</sup>), resulting in a threefold increase in SMX<sup>0</sup> sorption at pH 3 (5.849 mmol kg<sup>−1</sup> vs. 1.803 mmol kg<sup>−1</sup>). At pH 7, the sorption for SMX<sup>−</sup> reached 2.859 mmol kg<sup>−1</sup> for BC600, while no sorption was observed for BC400. Computational simulations indicated that sorption is spontaneous, endothermic, and primarily entropy-driven, governed by intraparticle diffusion. This work highlights the importance of pyrolysis temperature for enhancing SMX<sup>−</sup> sorption at neutral pH using pristine corncob biochar.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"29 ","pages":"Article 102050"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.biteb.2025.102026
Lakshana G. Nair, Pradeep Verma
The current study investigates the efficiency of a microwave (MW)-assisted Tetrahydrofuran-water (THF-H2O) pretreatment system on rice straw (RS) at low temperatures and time ranges. Experiments were performed at different temperatures and time ranges, and 100 °C and 40 min were optimized as the best pretreatment conditions due to high total reducing sugar yields (72.4 %). Under conventional heating (TR), the same conditions resulted in only 21.3 % total sugar yield. Composition analysis reveals an improved cellulose content in the MW-pretreated RS compared to untreated RS. Structural characterization by FTIR and XRD indicates lower crystallinity indices of the MW-pretreated RS. LC-HRMS analyses of the hydrolysate of MW- and TR-pretreated systems revealed the presence of twelve (12) and eight (8) platform chemicals, respectively. The presence of important platform chemicals and enhanced sugar yields marks the efficiency of the MW-assisted THF-H2O system for use in biorefineries with low temperature and time requirements.
{"title":"Pretreatment of rice straw using a microwave-assisted tetrahydrofuran-water system for enhanced sugar yield and generation of platform chemicals","authors":"Lakshana G. Nair, Pradeep Verma","doi":"10.1016/j.biteb.2025.102026","DOIUrl":"10.1016/j.biteb.2025.102026","url":null,"abstract":"<div><div>The current study investigates the efficiency of a microwave (MW)-assisted Tetrahydrofuran-water (THF-H<sub>2</sub>O) pretreatment system on rice straw (RS) at low temperatures and time ranges. Experiments were performed at different temperatures and time ranges, and 100 °C and 40 min were optimized as the best pretreatment conditions due to high total reducing sugar yields (72.4 %). Under conventional heating (TR), the same conditions resulted in only 21.3 % total sugar yield. Composition analysis reveals an improved cellulose content in the MW-pretreated RS compared to untreated RS. Structural characterization by FTIR and XRD indicates lower crystallinity indices of the MW-pretreated RS. LC-HRMS analyses of the hydrolysate of MW- and TR-pretreated systems revealed the presence of twelve (12) and eight (8) platform chemicals, respectively. The presence of important platform chemicals and enhanced sugar yields marks the efficiency of the MW-assisted THF-H<sub>2</sub>O system for use in biorefineries with low temperature and time requirements.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"29 ","pages":"Article 102026"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.biteb.2025.102046
Behnam Rezvani , Ahmad Hallajisani , Omid Tavakoli
This study investigates the co-pyrolysis of rice husk and sewage sludge to produce unmodified biochar (UBC) and modified biochar (MBC) for contaminant removal. The maximum alizarin red S (ARS) adsorption appeared in sewage sludge to rice husk ratio of 0.8:0.2 and pyrolysis temperature of 400 °C. The biochar was modified by acid treatment using hydrochloric acid, nitric acid, and phosphoric acid. Various factors of the adsorption were evaluated. The adsorbents had remarkable adsorption capacities. The maximum adsorption capacity of the MBCs reached 1466.38 to 1524.18 mg/g. The adsorption process followed pseudo-second-order kinetics and the Freundlich isotherm model. Fourier transform infrared (FTIR) analysis showed the diverse functional groups. Brunauer-Emmett-Teller (BET), Field emission scanning electron microscopy (FESEM), energy dispersive X-ray spectroscopy (EDS), and Thermogravimetric analysis (TGA) were utilized. Furthermore, the UBC was regenerated using solvents and an advanced technique. Additionally, the techno-economic significance of the biochar production was assessed.
{"title":"Super-effective biochar adsorbents from Co-pyrolysis of rice husk and sewage sludge: Adsorption performance, advanced regeneration, and economic analysis","authors":"Behnam Rezvani , Ahmad Hallajisani , Omid Tavakoli","doi":"10.1016/j.biteb.2025.102046","DOIUrl":"10.1016/j.biteb.2025.102046","url":null,"abstract":"<div><div>This study investigates the co-pyrolysis of rice husk and sewage sludge to produce unmodified biochar (UBC) and modified biochar (MBC) for contaminant removal. The maximum alizarin red S (ARS) adsorption appeared in sewage sludge to rice husk ratio of 0.8:0.2 and pyrolysis temperature of 400 °C. The biochar was modified by acid treatment using hydrochloric acid, nitric acid, and phosphoric acid. Various factors of the adsorption were evaluated. The adsorbents had remarkable adsorption capacities. The maximum adsorption capacity of the MBCs reached 1466.38 to 1524.18 mg/g. The adsorption process followed pseudo-second-order kinetics and the Freundlich isotherm model. Fourier transform infrared (FTIR) analysis showed the diverse functional groups. Brunauer-Emmett-Teller (BET), Field emission scanning electron microscopy (FESEM), energy dispersive X-ray spectroscopy (EDS), and Thermogravimetric analysis (TGA) were utilized. Furthermore, the UBC was regenerated using solvents and an advanced technique. Additionally, the techno-economic significance of the biochar production was assessed.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"29 ","pages":"Article 102046"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.biteb.2025.102032
Rajinikanth Rajagopal
High ammonia levels (>5 g N/L) have been a critical barrier in the anaerobic digestion (AD) process, with limited studies achieving long-term stability—until now. This research investigates strategies to counteract inhibition from free ammonia nitrogen (FAN) and short-chain fatty acids (SCFAs) in low-temperature AD (LT-AD) of nitrogen-rich feedstocks. LT-sequencing batch reactors (LT-SBRs) were tested under total ammonia nitrogen (TAN) concentrations up to 12.5 ± 1.56 g N/L at 24.5 ± 0.5 °C and 20 ± 0.5 °C. Results demonstrated that LT-SBR systems maintained stability, with VFA/alkalinity ratio below 1 and the propionic/acetic acid ratio ≤ 1.4. FAN/TAN conversion ratio decreased from 2.61 % at 24.5 °C to 1.17 % at 20 °C, ensuring minimal inhibition. Despite high TAN, methane production was resilient, with specific methane yields of 0.44 L CH4/gCOD at 24.5 °C and 0.22 L CH4/gCOD at 20 °C. These findings demonstrate that LT-AD can handle high‑nitrogen feedstocks, achieving robust methane yields and stable performance.
{"title":"Enhancing low-temperature anaerobic digestion of nitrogen-rich feedstocks: Mitigating free ammonia and short-chain fatty acid inhibitions","authors":"Rajinikanth Rajagopal","doi":"10.1016/j.biteb.2025.102032","DOIUrl":"10.1016/j.biteb.2025.102032","url":null,"abstract":"<div><div>High ammonia levels (>5 g N/L) have been a critical barrier in the anaerobic digestion (AD) process, with limited studies achieving long-term stability—until now. This research investigates strategies to counteract inhibition from free ammonia nitrogen (FAN) and short-chain fatty acids (SCFAs) in low-temperature AD (LT-AD) of nitrogen-rich feedstocks. LT-sequencing batch reactors (LT-SBRs) were tested under total ammonia nitrogen (TAN) concentrations up to 12.5 ± 1.56 g N/L at 24.5 ± 0.5 °C and 20 ± 0.5 °C. Results demonstrated that LT-SBR systems maintained stability, with VFA/alkalinity ratio below 1 and the propionic/acetic acid ratio ≤ 1.4. FAN/TAN conversion ratio decreased from 2.61 % at 24.5 °C to 1.17 % at 20 °C, ensuring minimal inhibition. Despite high TAN, methane production was resilient, with specific methane yields of 0.44 L CH<sub>4</sub>/gCOD at 24.5 °C and 0.22 L CH<sub>4</sub>/gCOD at 20 °C. These findings demonstrate that LT-AD can handle high‑nitrogen feedstocks, achieving robust methane yields and stable performance.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"29 ","pages":"Article 102032"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.biteb.2025.102040
Vaishali Kumar , Vandana Singh , Soumya Pandit
Contamination of the environment by heavy metals is a major problem on a worldwide scale. They are non-essential, nonbiodegradable and persistent in nature. Heavy metal contamination of soils and waterbodies has become a serious problem due to rapid industrialization and human activities including the uncontrolled use of agrochemicals, burning fossil fuels, and dumping sewage sludge. Bioremediation is a multifaceted approach for environmental cleanup that is effective, sustainable, safe, and inexpensive. Additionally, in-situ treatment is a key component of bioremediation technology that lessens the likelihood of contamination transmission to another location. Bioaccumulation, bioaugmentation, biotransformation, and biosorption are all included in the in-situ process. Microbial remediation demonstrates potential, with bacteria like Pseudomonas aeruginosa, Enterobacter cloacae B2-DHA, Klebsiella pneumoniae, Enterobacter sp. and Bacillus spp. showing effectiveness in metal detoxification. The inclination towards omics (genomics, proteomics, transcriptomics, etc.,) is to enhance the efficiency of the microorganisms which regulates the gene of interestand these diverse approaches constitute a critical stride towards alleviating the menace of heavy metal pollution and safeguarding the environment. This review focuses on the ecotoxicity of heavy metals and an advanced omics approach, strategies and sustainable remediation of heavy metals via microorganisms. Synergistic advantages with an unprecedented rise in heavy metal removal have been shown when these approaches are used as a sustainable environmental technology in the near future. This review emphasizes the essential significance of sophisticated omics techniques and microbiological methods in attaining sustainable and effective bioremediation, presenting a viable avenue for alleviating heavy metal contamination and safeguarding environmental health.
{"title":"Advanced omics approach and sustainable strategies for heavy metal microbial remediation in contaminated environments","authors":"Vaishali Kumar , Vandana Singh , Soumya Pandit","doi":"10.1016/j.biteb.2025.102040","DOIUrl":"10.1016/j.biteb.2025.102040","url":null,"abstract":"<div><div>Contamination of the environment by heavy metals is a major problem on a worldwide scale. They are non-essential, nonbiodegradable and persistent in nature. Heavy metal contamination of soils and waterbodies has become a serious problem due to rapid industrialization and human activities including the uncontrolled use of agrochemicals, burning fossil fuels, and dumping sewage sludge. Bioremediation is a multifaceted approach for environmental cleanup that is effective, sustainable, safe, and inexpensive. Additionally, in-situ treatment is a key component of bioremediation technology that lessens the likelihood of contamination transmission to another location. Bioaccumulation, bioaugmentation, biotransformation, and biosorption are all included in the in-situ process. Microbial remediation demonstrates potential, with bacteria like <em>Pseudomonas aeruginosa</em>, <em>Enterobacter cloacae</em> B2-DHA, <em>Klebsiella pneumoniae</em>, <em>Enterobacter</em> sp. and <em>Bacillus</em> spp. showing effectiveness in metal detoxification. The inclination towards omics (genomics, proteomics, transcriptomics, etc.,) is to enhance the efficiency of the microorganisms which regulates the gene of interestand these diverse approaches constitute a critical stride towards alleviating the menace of heavy metal pollution and safeguarding the environment. This review focuses on the ecotoxicity of heavy metals and an advanced omics approach, strategies and sustainable remediation of heavy metals via microorganisms. Synergistic advantages with an unprecedented rise in heavy metal removal have been shown when these approaches are used as a sustainable environmental technology in the near future. This review emphasizes the essential significance of sophisticated omics techniques and microbiological methods in attaining sustainable and effective bioremediation, presenting a viable avenue for alleviating heavy metal contamination and safeguarding environmental health.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"29 ","pages":"Article 102040"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.biteb.2025.102057
Bruno Chaves , Mélanie Salomez , Cecilia Sambusiti , Nicolas Thévenin , Laure Vieublé-Gonod , Céline Richard-Molard
The growing use of anaerobic digestion to treat organic waste and generate biogas has led to the production of large amounts of digestates. Digestates, nowadays valued as organic fertilizers and soil amendments, could also be a source of bioactive molecules with biostimulant characteristics. In this study, the digestates (raw and liquid), the humic and fulvic acids derived from raw digestate by alkali-acid extraction, their mixture (humic substances), as well as the water-soluble fraction of raw digestate, were evaluated for their biostimulant properties on winter rye. Potential biostimulant effects were compared to a commercial biostimulant and a nutrient solution during germination and growth of winter rye plants under hydroponic and soil conditions. The digestates did not show any significant biostimulant effect compared to the nutrient solution, while the water-soluble and fulvic acid extracts significantly increased root dry matter, root carbon and nitrogen quantities and projected leaf area of winter rye under hydroponic conditions. The root system architecture was differently modified by digestate and extract applications. While the raw digestate more than doubled the length of the thickest roots (diameter > 2 mm), the water-soluble extract promoted elongation of the thinnest roots (diameter < 0.3 mm) and enhanced total root length compared either with nutrient solution, commercial reference or digestates. The effects were more moderate on soil-grown rye plants but still existed. This work highlights water-soluble and fulvic acid extracts obtained from digestates as effective biostimulants for winter rye and, therefore, suggests a potential interest in using digestates as a source of biostimulants.
{"title":"Digestate as a source of biostimulants for winter rye growth","authors":"Bruno Chaves , Mélanie Salomez , Cecilia Sambusiti , Nicolas Thévenin , Laure Vieublé-Gonod , Céline Richard-Molard","doi":"10.1016/j.biteb.2025.102057","DOIUrl":"10.1016/j.biteb.2025.102057","url":null,"abstract":"<div><div>The growing use of anaerobic digestion to treat organic waste and generate biogas has led to the production of large amounts of digestates. Digestates, nowadays valued as organic fertilizers and soil amendments, could also be a source of bioactive molecules with biostimulant characteristics. In this study, the digestates (raw and liquid), the humic and fulvic acids derived from raw digestate by alkali-acid extraction, their mixture (humic substances), as well as the water-soluble fraction of raw digestate, were evaluated for their biostimulant properties on winter rye. Potential biostimulant effects were compared to a commercial biostimulant and a nutrient solution during germination and growth of winter rye plants under hydroponic and soil conditions. The digestates did not show any significant biostimulant effect compared to the nutrient solution, while the water-soluble and fulvic acid extracts significantly increased root dry matter, root carbon and nitrogen quantities and projected leaf area of winter rye under hydroponic conditions. The root system architecture was differently modified by digestate and extract applications. While the raw digestate more than doubled the length of the thickest roots (diameter > 2 mm), the water-soluble extract promoted elongation of the thinnest roots (diameter < 0.3 mm) and enhanced total root length compared either with nutrient solution, commercial reference or digestates. The effects were more moderate on soil-grown rye plants but still existed. This work highlights water-soluble and fulvic acid extracts obtained from digestates as effective biostimulants for winter rye and, therefore, suggests a potential interest in using digestates as a source of biostimulants.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"29 ","pages":"Article 102057"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143142739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.biteb.2025.102054
Ponmanian M, Sivashanmugam P
Cheese whey wastewater (CWW) generated during cheese manufacturing poses an environmental issue due to its high concentration of organic matter and contaminants, including nitrogen and phosphorus. These can lead to eutrophication and represent hazards to human health. The objective of this study was to develop a biological method for the sustainable and efficient treatment of CWW using native bacterial pure isolates and mixed cultures. This method also aims to enhance circular economy practices in the dairy industry by adopting a biorefinery approach to produce biopolymer from the treated biomass. Hence, the objective was to decrease the contaminants in the wastewater that would allow its reuse in agricultural activities. CWW was obtained from a dairy farm in India and used as a substrate for the growth of bacteria. In this work, three promising isolates were screened from a pool of nineteen microorganisms for CWW treatment based on their growth in cheese whey effluent. After 7 days of CWW treatment with mixed culture cells, it was approximated that the procedure could achieve a removal efficiency of 89.10 % for COD, 78.35 % for total nitrogen, and 83.77 % for total phosphorus in CWW. Polyhydroxyalkanoates (PHA) were isolated from the bioremediated biomass and the mixed culture (CW123) generated 389.23 mg/L of PHA. Analytical analysis has revealed that the extracted PHA have properties similar to commercial PHA. Hence, this strategy has demonstrated the capacity to reduce contaminants in CWW and provide a sustainable source for producing PHA, promoting a circular economy in the dairy sector.
{"title":"Biotransformation of cheese whey effluent: A dual approach for wastewater treatment and polyhydroxyalkanoates production using mixed microbial cultures","authors":"Ponmanian M, Sivashanmugam P","doi":"10.1016/j.biteb.2025.102054","DOIUrl":"10.1016/j.biteb.2025.102054","url":null,"abstract":"<div><div>Cheese whey wastewater (CWW) generated during cheese manufacturing poses an environmental issue due to its high concentration of organic matter and contaminants, including nitrogen and phosphorus. These can lead to eutrophication and represent hazards to human health. The objective of this study was to develop a biological method for the sustainable and efficient treatment of CWW using native bacterial pure isolates and mixed cultures. This method also aims to enhance circular economy practices in the dairy industry by adopting a biorefinery approach to produce biopolymer from the treated biomass. Hence, the objective was to decrease the contaminants in the wastewater that would allow its reuse in agricultural activities. CWW was obtained from a dairy farm in India and used as a substrate for the growth of bacteria. In this work, three promising isolates were screened from a pool of nineteen microorganisms for CWW treatment based on their growth in cheese whey effluent. After 7 days of CWW treatment with mixed culture cells, it was approximated that the procedure could achieve a removal efficiency of 89.10 % for COD, 78.35 % for total nitrogen, and 83.77 % for total phosphorus in CWW. Polyhydroxyalkanoates (PHA) were isolated from the bioremediated biomass and the mixed culture (CW<sub>123</sub>) generated 389.23 mg/L of PHA. Analytical analysis has revealed that the extracted PHA have properties similar to commercial PHA. Hence, this strategy has demonstrated the capacity to reduce contaminants in CWW and provide a sustainable source for producing PHA, promoting a circular economy in the dairy sector.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"29 ","pages":"Article 102054"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.biteb.2024.102009
Edward D. Nasser , Joel Kenneth M. Ngojo , Ramiro Emerson C. Amon , Jayvee Moore S. Dy , Julius J. Rosalia , Chosel P. Lawagon
Biogas digestate, though nutrient-rich, is underutilized due to its unpleasant odor and limited efficacy as fertilizer. This study synthesized an encapsulated nanofertilizer (ENF) using polyurethane (PU) to enhance nutrient release control. Nitrate release studies showed that ENF delayed significant nutrient release to 10.57 days, aligning with controlled-release fertilizer standards by achieving a maximum of 65 % nutrient release over 30 days. Nutrient release kinetics fitted the Korsmeyer-Peppas model (R2 = 0.9984), confirming diffusion-controlled Fickian release and a matrix-based system. Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX) analysis confirmed the matrix structure, while Fourier Transform Infrared Spectroscopy (FTIR) verified the successful synthesis of PU coating. These findings suggest that encapsulated nanofertilizer improves nutrient release profiles, offering a sustainable solution for digestate valorization.
{"title":"Synthesis of encapsulated nanofertilizer from biogas plant digestate","authors":"Edward D. Nasser , Joel Kenneth M. Ngojo , Ramiro Emerson C. Amon , Jayvee Moore S. Dy , Julius J. Rosalia , Chosel P. Lawagon","doi":"10.1016/j.biteb.2024.102009","DOIUrl":"10.1016/j.biteb.2024.102009","url":null,"abstract":"<div><div>Biogas digestate, though nutrient-rich, is underutilized due to its unpleasant odor and limited efficacy as fertilizer. This study synthesized an encapsulated nanofertilizer (ENF) using polyurethane (PU) to enhance nutrient release control. Nitrate release studies showed that ENF delayed significant nutrient release to 10.57 days, aligning with controlled-release fertilizer standards by achieving a maximum of 65 % nutrient release over 30 days. Nutrient release kinetics fitted the Korsmeyer-Peppas model (<em>R</em><sup><em>2</em></sup> = 0.9984), confirming diffusion-controlled Fickian release and a matrix-based system. Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX) analysis confirmed the matrix structure, while Fourier Transform Infrared Spectroscopy (FTIR) verified the successful synthesis of PU coating. These findings suggest that encapsulated nanofertilizer improves nutrient release profiles, offering a sustainable solution for digestate valorization.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"29 ","pages":"Article 102009"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143141913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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