The rising demand for high-value products has sparked interest in producing microbial oils and β-carotene. Simultaneously, advances in microbial processing have accelerated the utilization of alternative renewable resources. Biodiesel-based crude glycerol (BCG) has been valorized through yeast cultivation for bio-polyurethane (BPU) and β-carotene synthesis. Under continuous fermentation, the highest glycerol consumption, biomass, yeast oil (YO), and carotene production were achieved at a dilution rate of 0.005 h−1. The cell-recycled continuous fermentation (CR-CF) strategy enhanced the volumetric productivity of yeast biomass (QX; 0.056 g/L·h), oil (QYO; 0.033 g/L·h), and β-carotene (QC; 0.207 mg/L·h) by 1.81-, 2.36-, and 1.07-fold, respectively compared to conventional CF, with 76.6% glycerol consumption efficiency. YO was successfully converted to semi-rigid BPU, with a density of 0.98 g/cm3 and 47.60% water absorption. β-Carotene extract encapsulated by spray drying with gum Arabic and maltodextrin provided high solubility, photostability, and antimicrobial activity. These results demonstrated that BCG was an effective and sustainable alternative feedstock for co-producing valuable microbial products. The CR-CF system showed promise for improving production efficiency. Additionally, the value upgrading strategy of YO and β-carotene was an attractive process for environmentally friendly and sustainable biological product formation.
{"title":"Effective simultaneous production of yeast oil and β-carotene from biodiesel-based crude glycerol in a cell-recycled continuous fermentation system producing bio-polyurethane and encapsulated β-carotene","authors":"Sasitorn Khuntong , Parichat Koedprasong , Pensri Plangklang , Thidarat Samranrit , Witsanu Srila , Surachai Techaoei , Apinan Wanlapa , Kanungnid Busarakam , Atsadawut Areesirisuk","doi":"10.1016/j.biteb.2026.102556","DOIUrl":"10.1016/j.biteb.2026.102556","url":null,"abstract":"<div><div>The rising demand for high-value products has sparked interest in producing microbial oils and β-carotene. Simultaneously, advances in microbial processing have accelerated the utilization of alternative renewable resources. Biodiesel-based crude glycerol (BCG) has been valorized through yeast cultivation for bio-polyurethane (BPU) and β-carotene synthesis. Under continuous fermentation, the highest glycerol consumption, biomass, yeast oil (YO), and carotene production were achieved at <em>a dilution rate of</em> 0.005 h<sup>−1</sup>. The cell-recycled continuous fermentation (CR-CF) strategy enhanced the volumetric productivity of yeast biomass (<em>Q</em><sub>X</sub>; 0.056 g/L·h), oil (<em>Q</em><sub>YO</sub>; 0.033 g/L·h), and β-carotene (<em>Q</em><sub>C</sub>; 0.207 mg/L·h) by 1.81-, 2.36-, and 1.07-fold, respectively compared to conventional CF, with 76.6% glycerol consumption efficiency. YO was successfully converted to semi-rigid BPU, with a density of 0.98 g/cm<sup>3</sup> and 47.60% water absorption. β-Carotene extract encapsulated by spray drying with gum Arabic and maltodextrin provided high solubility, photostability, and antimicrobial activity. These results demonstrated that BCG was an effective and sustainable alternative feedstock for co-producing valuable microbial products. The CR-CF system showed promise for improving production efficiency. Additionally, the value upgrading strategy of YO and β-carotene was an attractive process for environmentally friendly and sustainable biological product formation.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102556"},"PeriodicalIF":0.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-06DOI: 10.1016/j.biteb.2025.102535
Xiaoxuan Zhou , Zhengdong Wan , Ran Gao , Jianxiong Hao , Wei Wang , Xuechao Zheng , Dandan Zhao
Phycocyanin (PC), a bioactive pigment-protein complex found in cyanobacteria, has gained significant attention due to its diverse applications in food, pharmaceuticals, and cosmetics, particularly as a natural blue colorant and nutritional supplement in food products. This review summarizes regulatory mechanisms and enhancement strategies for PC accumulation, focusing on single/combined exogenous substances and photodynamic synergy. These interventions regulate PC synthesis mainly via energy optimization, metabolic balance, and cellular protection. Challenges like high costs and scalability remain. Future research should develop industrial by-products/agricultural waste as low-cost exogenous substances, optimize intelligent photobioreactors (PBRs), apply genetic engineering/mutagenesis to break PC synthesis limits, and integrate transcriptomics, proteomics, and metabolomics to boost industrial efficiency. This review provides a theoretical basis for PC development, supporting the sustainable bioeconomy and the advancement of the protein industry.
{"title":"Exploration of the pathway and mechanism of phycocyanin accumulation: A review","authors":"Xiaoxuan Zhou , Zhengdong Wan , Ran Gao , Jianxiong Hao , Wei Wang , Xuechao Zheng , Dandan Zhao","doi":"10.1016/j.biteb.2025.102535","DOIUrl":"10.1016/j.biteb.2025.102535","url":null,"abstract":"<div><div>Phycocyanin (PC), a bioactive pigment-protein complex found in cyanobacteria, has gained significant attention due to its diverse applications in food, pharmaceuticals, and cosmetics, particularly as a natural blue colorant and nutritional supplement in food products. This review summarizes regulatory mechanisms and enhancement strategies for PC accumulation, focusing on single/combined exogenous substances and photodynamic synergy. These interventions regulate PC synthesis mainly via energy optimization, metabolic balance, and cellular protection. Challenges like high costs and scalability remain. Future research should develop industrial by-products/agricultural waste as low-cost exogenous substances, optimize intelligent photobioreactors (PBRs), apply genetic engineering/mutagenesis to break PC synthesis limits, and integrate transcriptomics, proteomics, and metabolomics to boost industrial efficiency. This review provides a theoretical basis for PC development, supporting the sustainable bioeconomy and the advancement of the protein industry.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102535"},"PeriodicalIF":0.0,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145922213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This research article aimed to develop biodegradable chitosan-gelatin composite films reinforced with natural wax and silver nanoparticles (AgNPs), indicating considerable mechanical, barrier, antimicrobial, and heat-induced sealing. The films showed a great tensile strength exceeding 58 MPa, significantly enhanced water contact angles up to 117.5°, revealing superior hydrophobicity, and a thermal healing efficiency reaching 82.4 % after thermal annealing. The integration of AgNPs enhanced not only the antimicrobial activity against Gram-positive and Gram-negative bacteria but also the mechanical performance and water vapor barrier properties of the films. The thermally triggered repair capability of the films stems from temperature-driven translocation of incorporated natural wax to superficial regions, which effectively restores both hydrophobicity and mechanical integrity following induced damage. These results propose that the developed composite films are promising candidates for sustainable packaging applications as environmentally friendly alternatives to synthetic plastics.
{"title":"Thermally repairable and antimicrobial chitosan-gelatin films with natural wax and silver nanoparticles","authors":"Neda Aliabbasi , Zahra Emam-Djomeh , Hassan Rezaeinia","doi":"10.1016/j.biteb.2025.102533","DOIUrl":"10.1016/j.biteb.2025.102533","url":null,"abstract":"<div><div>This research article aimed to develop biodegradable chitosan-gelatin composite films reinforced with natural wax and silver nanoparticles (AgNPs), indicating considerable mechanical, barrier, antimicrobial, and heat-induced sealing. The films showed a great tensile strength exceeding 58 MPa, significantly enhanced water contact angles up to 117.5°, revealing superior hydrophobicity, and a thermal healing efficiency reaching 82.4 % after thermal annealing. The integration of AgNPs enhanced not only the antimicrobial activity against Gram-positive and Gram-negative bacteria but also the mechanical performance and water vapor barrier properties of the films. The thermally triggered repair capability of the films stems from temperature-driven translocation of incorporated natural wax to superficial regions, which effectively restores both hydrophobicity and mechanical integrity following induced damage. These results propose that the developed composite films are promising candidates for sustainable packaging applications as environmentally friendly alternatives to synthetic plastics.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102533"},"PeriodicalIF":0.0,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-06DOI: 10.1016/j.biteb.2025.102525
Sefiu Olaitan Amusat , Simiso Dube , Jabulani I. Mnyango , Shanganyane P. Hlangothi , Bienvenu Gael Fouda Mbanga , Seyisi Thulethu , Sabelo Mhlanga , Zikhona Tywabi-Ngeva
The reinforcement of biodegradable mulching films with biochar represents a promising strategy to enhance the performance, sustainability, and environmental compatibility of water and nutrient management in agricultural practices. To guarantee food security, sustainable water management techniques must be developed. As water scarcity intensifies under changing climate conditions, there is an urgent need for environmentally responsible practices that enhance soil–water dynamics while supporting crop productivity. Recently, biodegradable mulching films have emerged as a promising, eco-friendly alternative to traditional plastic mulches. However, their mechanical strength, degradation behaviors, and long-term field stability remain key limitations. In this review, we critically examine and evaluate the potential of biochar-reinforced biodegradable mulching films as a next-generation solution for sustainable agriculture. Various types, properties, and mechanisms of biodegradable mulching films were discussed in this review. We also highlighted the prospect of application of biochar-reinforced biodegradable mulching films, highlighting their potential to address pressing challenges in climate-resilient and sustainable agriculture. We provided the synergistic advantages of biochar in enhancing the mechanical characteristics, thermal stability, water retention, and nutrient management of mulching films, while also contributing to carbon sequestration and soil health. This review emphasizes a novel pathway to developing cutting-edge mulching technologies that can more effectively promote climate-resilient food production by bridging knowledge across soil science, materials engineering, and climate-smart agriculture.
{"title":"Biochar-reinforced biodegradable mulching films: Toward climate-resilient and sustainable agriculture","authors":"Sefiu Olaitan Amusat , Simiso Dube , Jabulani I. Mnyango , Shanganyane P. Hlangothi , Bienvenu Gael Fouda Mbanga , Seyisi Thulethu , Sabelo Mhlanga , Zikhona Tywabi-Ngeva","doi":"10.1016/j.biteb.2025.102525","DOIUrl":"10.1016/j.biteb.2025.102525","url":null,"abstract":"<div><div>The reinforcement of biodegradable mulching films with biochar represents a promising strategy to enhance the performance, sustainability, and environmental compatibility of water and nutrient management in agricultural practices. To guarantee food security, sustainable water management techniques must be developed. As water scarcity intensifies under changing climate conditions, there is an urgent need for environmentally responsible practices that enhance soil–water dynamics while supporting crop productivity. Recently, biodegradable mulching films have emerged as a promising, eco-friendly alternative to traditional plastic mulches. However, their mechanical strength, degradation behaviors, and long-term field stability remain key limitations. In this review, we critically examine and evaluate the potential of biochar-reinforced biodegradable mulching films as a next-generation solution for sustainable agriculture. Various types, properties, and mechanisms of biodegradable mulching films were discussed in this review. We also highlighted the prospect of application of biochar-reinforced biodegradable mulching films, highlighting their potential to address pressing challenges in climate-resilient and sustainable agriculture. We provided the synergistic advantages of biochar in enhancing the mechanical characteristics, thermal stability, water retention, and nutrient management of mulching films, while also contributing to carbon sequestration and soil health. This review emphasizes a novel pathway to developing cutting-edge mulching technologies that can more effectively promote climate-resilient food production by bridging knowledge across soil science, materials engineering, and climate-smart agriculture.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102525"},"PeriodicalIF":0.0,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145921526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-06DOI: 10.1016/j.biteb.2025.102517
Birupakshya Mishra , Sanjana Pal , U. Jayakrishnan , Chalicheemala Srinath , Subhankar Roy , Siddhartha Moulik , Alka Kumari
The present work demonstrates the technological advancement in the resource recovery approaches to extract a high-value product, pectin, from sweet lime peel (SLP) using Choline chloride: Citric acid deep eutectic solvent (DES) and a novel rotating hydrodynamic cavitation (RHC) reactor. The present work employs a low-carbon circular bio-economy by combining RHC technology for the valorization of SLP through pectin extraction. Numerical simulations using computational fluid dynamics revealed the optimum geometrical parameters for the cavitation generation unit to be of semi-circular shape, 9 mm depth, 10 mm diameter with 504 units. Box–Behnken Design of pectin extraction using numerically optimized RHC indicated that time of 32.8 min, rotational speed of 1800 rpm, solid: liquid ratio of 1:30, at fixed DES: water ratio of 1:6, yielded maximum pectin of 32.89 % with 88.24 % degree of esterification. The Analysis of Variance assessment indicated the influence of process parameters in the order rotational speed>extraction time > solid-to-liquid ratio. The physicochemical characterization of the RHC-extracted pectin observed similar morphology and structure to that of commercial pectin. The process developed ensured higher pectin yield having high thermal stability but resulted in possible truncation of pectin chains into oligomers with molecular weight 60.04–901.10 kDa. The scale-up study has demonstrated the feasibility of an industrial scale process and its cost-effective aspects with a significant return on investment within a year.
{"title":"Hydrodynamic cavitation-assisted intensified extraction of pectin from sweet lime peels using deep eutectic solvent","authors":"Birupakshya Mishra , Sanjana Pal , U. Jayakrishnan , Chalicheemala Srinath , Subhankar Roy , Siddhartha Moulik , Alka Kumari","doi":"10.1016/j.biteb.2025.102517","DOIUrl":"10.1016/j.biteb.2025.102517","url":null,"abstract":"<div><div>The present work demonstrates the technological advancement in the resource recovery approaches to extract a high-value product, pectin, from sweet lime peel (SLP) using Choline chloride: Citric acid deep eutectic solvent (DES) and a novel rotating hydrodynamic cavitation (RHC) reactor. The present work employs a low-carbon circular bio-economy by combining RHC technology for the valorization of SLP through pectin extraction. Numerical simulations using computational fluid dynamics revealed the optimum geometrical parameters for the cavitation generation unit to be of semi-circular shape, 9 mm depth, 10 mm diameter with 504 units. Box–Behnken Design of pectin extraction using numerically optimized RHC indicated that time of 32.8 min, rotational speed of 1800 rpm, solid: liquid ratio of 1:30, at fixed DES: water ratio of 1:6, yielded maximum pectin of 32.89 % with 88.24 % degree of esterification. The Analysis of Variance assessment indicated the influence of process parameters in the order rotational speed>extraction time > solid-to-liquid ratio. The physicochemical characterization of the RHC-extracted pectin observed similar morphology and structure to that of commercial pectin. The process developed ensured higher pectin yield having high thermal stability but resulted in possible truncation of pectin chains into oligomers with molecular weight 60.04–901.10 kDa. The scale-up study has demonstrated the feasibility of an industrial scale process and its cost-effective aspects with a significant return on investment within a year.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102517"},"PeriodicalIF":0.0,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-06DOI: 10.1016/j.biteb.2025.102512
Jyoti Moni Devi , Biplab Kumar Debnath , Rajat Subhra Das , Biplab Das
Anaerobic digestion (AD) is a well-established biological process used to convert and treat complex substrates. However, its widespread implementation is constrained by several challenges. The use of additives such as biochar (BC) is proposed to address these challenges. This review summarizes the applications of BC in AD, including parent materials for BC-assisted AD systems, substrates used in AD, BC's function, including parent materials for BC-assisted AD systems, BC's role in AD, engineered BC-assisted AD approaches, and its application in biogas purification. Additionally, to address the expansion of BC incorporation, a strengths-weaknesses-opportunities-threats (SWOT) analysis is conducted. Despite the numerous benefits associated with BC, its cost-effectiveness compared to energy gains is vital. A key issue identified is the lack of large-scale studies. Limited studies that have examined the long-term regulatory effects of BC in full-scale reactors. Future research can concentrate on optimizing BC performance and refining production methods to enhance cost-effectiveness. Effective policies can incentivize the use of biogas and BC expertise, endorsing their broader application.
{"title":"Biochar integration in biomethanation process: Addressing limitations and unlocking performance gains","authors":"Jyoti Moni Devi , Biplab Kumar Debnath , Rajat Subhra Das , Biplab Das","doi":"10.1016/j.biteb.2025.102512","DOIUrl":"10.1016/j.biteb.2025.102512","url":null,"abstract":"<div><div>Anaerobic digestion (AD) is a well-established biological process used to convert and treat complex substrates. However, its widespread implementation is constrained by several challenges. The use of additives such as biochar (BC) is proposed to address these challenges. This review summarizes the applications of BC in AD, including parent materials for BC-assisted AD systems, substrates used in AD, BC's function, including parent materials for BC-assisted AD systems, BC's role in AD, engineered BC-assisted AD approaches, and its application in biogas purification. Additionally, to address the expansion of BC incorporation, a strengths-weaknesses-opportunities-threats (SWOT) analysis is conducted. Despite the numerous benefits associated with BC, its cost-effectiveness compared to energy gains is vital. A key issue identified is the lack of large-scale studies. Limited studies that have examined the long-term regulatory effects of BC in full-scale reactors. Future research can concentrate on optimizing BC performance and refining production methods to enhance cost-effectiveness. Effective policies can incentivize the use of biogas and BC expertise, endorsing their broader application.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102512"},"PeriodicalIF":0.0,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-05DOI: 10.1016/j.biteb.2025.102516
Sujata Phurailatpam , Meena Khwairakpam
The significance of microorganisms in composting is well-established, but the ecological role of invertebrates has received comparatively less attention. The present study addresses this gap by examining the abundance and diversity of invertebrates and their correlations with physicochemical and biological parameters during pile composting. Composting was carried out using a blended substrate of vegetable waste, cow dung, and sawdust. The invertebrates from the waste biomass were extracted using a modified Berlese Tullgren Funnel. During the study, a total of 11,344 mesofauna and macrofauna individuals were collected and identified during the pile composting. Springtails dominated the community, followed by mites and black soldier fly larvae, underscoring the central role of these taxa in waste decomposition. Diversity analyses using the Shannon–Wiener and Simpson indices revealed clear associations between community structure and compost dynamics. In addition, the physicochemical and biological parameters of the waste biomass agreed with the variation of abundance of the invertebrates during the process. The highest abundance of invertebrates was observed within the temperature range of 33.5 °C to 37.3 °C, and corresponding moisture content from 35.24 % to 38.01 % conditions favourable for both decomposition and faunal activity. These findings highlight invertebrates as active drivers of compost transformation, bridging biological activity with physicochemical processes. The study provides a foundation for the strategic utilization of key invertebrate taxa to enhance feedstock decomposition and improve composting efficiency.
{"title":"Hidden engineers of decomposition: Invertebrate communities and their association with composting parameters in vegetable waste pile","authors":"Sujata Phurailatpam , Meena Khwairakpam","doi":"10.1016/j.biteb.2025.102516","DOIUrl":"10.1016/j.biteb.2025.102516","url":null,"abstract":"<div><div>The significance of microorganisms in composting is well-established, but the ecological role of invertebrates has received comparatively less attention. The present study addresses this gap by examining the abundance and diversity of invertebrates and their correlations with physicochemical and biological parameters during pile composting. Composting was carried out using a blended substrate of vegetable waste, cow dung, and sawdust. The invertebrates from the waste biomass were extracted using a modified Berlese Tullgren Funnel. During the study, a total of 11,344 mesofauna and macrofauna individuals were collected and identified during the pile composting. Springtails dominated the community, followed by mites and black soldier fly larvae, underscoring the central role of these taxa in waste decomposition. Diversity analyses using the Shannon–Wiener and Simpson indices revealed clear associations between community structure and compost dynamics. In addition, the physicochemical and biological parameters of the waste biomass agreed with the variation of abundance of the invertebrates during the process. The highest abundance of invertebrates was observed within the temperature range of 33.5 °C to 37.3 °C, and corresponding moisture content from 35.24 % to 38.01 % conditions favourable for both decomposition and faunal activity. These findings highlight invertebrates as active drivers of compost transformation, bridging biological activity with physicochemical processes. The study provides a foundation for the strategic utilization of key invertebrate taxa to enhance feedstock decomposition and improve composting efficiency.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102516"},"PeriodicalIF":0.0,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-05DOI: 10.1016/j.biteb.2026.102547
Samsul Rizal , Maria Erna Kustyawati , Novita Herdiana , Dewi Sartika , Fairuzsita Naura Amalia Syifani , Honi Aisya , Dita Artha Ristiani , Ayu Dian Pratiwi Permatahati
Mosaccha tempeh is a soybean-based fermented product produced using a mixed-culture inoculum of Rhizopus oligosporus and Saccharomyces cerevisiae, which enhances its nutritional value and sensory properties compared with conventional fermentation. Despite these benefits, its shelf life remains limited due to rapid microbial activity at ambient temperatures. This study evaluated the effects of vacuum and non-vacuum packaging under room and cold storage conditions on the quality and shelf life of Mosaccha tempeh. Tempeh was stored for four days at room temperature and seven days under refrigeration, with daily measurements of water content, hardness, soluble protein, and sensory attributes. Data were analyzed using ANOVA followed by the Honest Significant Difference (HSD) test at the 5 % significance level. Under room-temperature storage, non-vacuum packaging maintained acceptable sensory quality for up to two days, with corresponding values of 65.37 % water content, 45.80 % soluble protein, and 580.08 gf hardness, and the product remained consumable through day three. Under cold storage, vacuum packaging preserved quality more effectively, maintaining sensory acceptability for up to four days, supported by 61.52 % water content, 41.82 % soluble protein, and 469.25 gf hardness, with acceptability extending to day six. These findings provide practical guidance for producers of Mosaccha tempeh in selecting suitable packaging and storage conditions and for consumers to maximize product quality and shelf life. Overall, this study demonstrates that Mosaccha tempeh is a viable alternative fermented product with competitive shelf-life characteristics, supporting its potential for wider distribution and value-added applications.
{"title":"The shelf life of Mosaccha tempeh under different packaging conditions and storage temperatures","authors":"Samsul Rizal , Maria Erna Kustyawati , Novita Herdiana , Dewi Sartika , Fairuzsita Naura Amalia Syifani , Honi Aisya , Dita Artha Ristiani , Ayu Dian Pratiwi Permatahati","doi":"10.1016/j.biteb.2026.102547","DOIUrl":"10.1016/j.biteb.2026.102547","url":null,"abstract":"<div><div>Mosaccha tempeh is a soybean-based fermented product produced using a mixed-culture inoculum of <em>Rhizopus oligosporus</em> and <em>Saccharomyces cerevisiae</em>, which enhances its nutritional value and sensory properties compared with conventional fermentation. Despite these benefits, its shelf life remains limited due to rapid microbial activity at ambient temperatures. This study evaluated the effects of vacuum and non-vacuum packaging under room and cold storage conditions on the quality and shelf life of Mosaccha tempeh. Tempeh was stored for four days at room temperature and seven days under refrigeration, with daily measurements of water content, hardness, soluble protein, and sensory attributes. Data were analyzed using ANOVA followed by the Honest Significant Difference (HSD) test at the 5 % significance level. Under room-temperature storage, non-vacuum packaging maintained acceptable sensory quality for up to two days, with corresponding values of 65.37 % water content, 45.80 % soluble protein, and 580.08 gf hardness, and the product remained consumable through day three. Under cold storage, vacuum packaging preserved quality more effectively, maintaining sensory acceptability for up to four days, supported by 61.52 % water content, 41.82 % soluble protein, and 469.25 gf hardness, with acceptability extending to day six. These findings provide practical guidance for producers of Mosaccha tempeh in selecting suitable packaging and storage conditions and for consumers to maximize product quality and shelf life. Overall, this study demonstrates that Mosaccha tempeh is a viable alternative fermented product with competitive shelf-life characteristics, supporting its potential for wider distribution and value-added applications.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102547"},"PeriodicalIF":0.0,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145922147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-05DOI: 10.1016/j.biteb.2025.102530
Subhrajit Roy, Bhakti Godbole, Prakash Biswas
This study investigated the role of the hexaaquo complex ([Cu(H2O)6]2+) in the microwave-assisted conversion of lignocellulosic pine needles (Pinus roxburghii) to 5-hydroxymethylfurfural (HMF) using triethylammonium hydrogen sulfate as a protic ionic liquid (PIL). In the reaction medium, cellulose was hydrolyzed into monomeric sugars, such as glucose and fructose, in the presence of PIL–water and copper chloride catalysts. The polar [Cu(H2O)6]2+ complex interacted with cellulose, facilitating bond cleavage and subsequent dehydration reactions. Under microwave irradiation, in the presence of CuCl2 catalyst in the ionic liquid medium, glucose dehydrated to HMF via the elimination of water molecules. Acid hydrolysis, facilitated by the formation of in-situ HCl in the PIL, helped to break the β-1,4-glycosidic bonds in cellulose. Additionally, the presence of free chloride ions in the PIL–CuCl2 system enhanced glucose-to-fructose isomerization, a key reaction step in maximizing HMF yield. A comparatively moderate HMF yield of 16 % was achieved under these conditions, while the absence of free chloride ions led to a lower yield (∼1.8 %), highlighting the regulatory role of chloride ion availability. The study emphasized the dual catalytic role of the [Cu(H2O)6]2+ complex in both hydrolysis and dehydration pathways. A detailed kinetic modelling study was conducted to evaluate rate constants and activation energies, providing insights into the influence of the [Cu(H2O)6]2+ complex and microwave energy on reaction efficiency and selectivity.
{"title":"Role of hexa-aquo copper complex in microwave-assisted catalytic conversion of pine needles to glucose and 5-hydroxymethylfurfural in the presence of protic ionic liquid: Experimental and kinetic study","authors":"Subhrajit Roy, Bhakti Godbole, Prakash Biswas","doi":"10.1016/j.biteb.2025.102530","DOIUrl":"10.1016/j.biteb.2025.102530","url":null,"abstract":"<div><div>This study investigated the role of the hexaaquo complex ([Cu(H<sub>2</sub>O)<sub>6</sub>]<sup>2+</sup>) in the microwave-assisted conversion of lignocellulosic pine needles (<em>Pinus roxburghii</em>) to 5-hydroxymethylfurfural (HMF) using triethylammonium hydrogen sulfate as a protic ionic liquid (PIL). In the reaction medium, cellulose was hydrolyzed into monomeric sugars, such as glucose and fructose, in the presence of PIL–water and copper chloride catalysts. The polar [Cu(H<sub>2</sub>O)<sub>6</sub>]<sup>2+</sup> complex interacted with cellulose, facilitating bond cleavage and subsequent dehydration reactions. Under microwave irradiation, in the presence of CuCl<sub>2</sub> catalyst in the ionic liquid medium, glucose dehydrated to HMF via the elimination of water molecules. Acid hydrolysis, facilitated by the formation of in-situ HCl in the PIL, helped to break the β-1,4-glycosidic bonds in cellulose. Additionally, the presence of free chloride ions in the PIL–CuCl<sub>2</sub> system enhanced glucose-to-fructose isomerization, a key reaction step in maximizing HMF yield. A comparatively moderate HMF yield of 16 % was achieved under these conditions, while the absence of free chloride ions led to a lower yield (∼1.8 %), highlighting the regulatory role of chloride ion availability. The study emphasized the dual catalytic role of the [Cu(H<sub>2</sub>O)<sub>6</sub>]<sup>2+</sup> complex in both hydrolysis and dehydration pathways. A detailed kinetic modelling study was conducted to evaluate rate constants and activation energies, providing insights into the influence of the [Cu(H<sub>2</sub>O)<sub>6</sub>]<sup>2+</sup> complex and microwave energy on reaction efficiency and selectivity.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102530"},"PeriodicalIF":0.0,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-05DOI: 10.1016/j.biteb.2025.102534
Priya Mohan , Fauziah Shahul Hamid
Landfilled plastics serve as an untapped carbon reservoir, fostering selective conditions that enable microbial communities to compete and metabolize the available polymer-derived carbon. This study aims to evaluate the biodegradation potential of fungal strains isolated from Jeram Sanitary Landfill on PET, HDPE, and LDPE plastics. A total of eight fungal strains were isolated from plastic waste and tested in two liquid media, namely modified Biochemical Oxygen Demand Dilution Water (BODW) and Bushnell Haas media to determine the optimal media for degradation. Among the isolates, Aspergillus fumigatus (FI 5) exhibited the highest degradation efficiency, achieving 1.8 % and 2.0 % in BODW and Bushnell Haas media, respectively. A cost-benefit analysis identified BODW media as the more economical media, warranting further optimization. Degradation efficiency was significantly influenced by fungal inoculum concentration, where 1 % (w/v) inoculum yielded the highest PET weight loss (1.49 %), while a 5 % (w/v) inoculum resulted in the highest LDPE (21.93 %) and HDPE (2.79 %) weight reduction after 30 days of incubation. FTIR analysis revealed clear structural alterations in the plastic films, confirming active biodegradation. PET and LDPE degradation showed strong statistical responses (p = 0.0010 and p = 0.0100) driven mainly by incubation time. The lack-of-fit across all models (PET: 0.7599; HDPE: 0.9281; LDPE: 0.9918) further validated model reliability. Collectively, these findings highlight the potential of A. fumigatus to degrade PET, HDPE, and LDPE in BODW, supporting its use as an alternative approach for future plastic biodegradation.
{"title":"Biodegradation of PET and PE by tropical landfill derived Aspergillus fumigatus in modified BOD enriched medium","authors":"Priya Mohan , Fauziah Shahul Hamid","doi":"10.1016/j.biteb.2025.102534","DOIUrl":"10.1016/j.biteb.2025.102534","url":null,"abstract":"<div><div>Landfilled plastics serve as an untapped carbon reservoir, fostering selective conditions that enable microbial communities to compete and metabolize the available polymer-derived carbon. This study aims to evaluate the biodegradation potential of fungal strains isolated from Jeram Sanitary Landfill on PET, HDPE, and LDPE plastics. A total of eight fungal strains were isolated from plastic waste and tested in two liquid media, namely modified Biochemical Oxygen Demand Dilution Water (BODW) and Bushnell Haas media to determine the optimal media for degradation. Among the isolates, <em>Aspergillus fumigatus</em> (FI 5) exhibited the highest degradation efficiency, achieving 1.8 % and 2.0 % in BODW and Bushnell Haas media, respectively. A cost-benefit analysis identified BODW media as the more economical media, warranting further optimization. Degradation efficiency was significantly influenced by fungal inoculum concentration, where 1 % (w/v) inoculum yielded the highest PET weight loss (1.49 %), while a 5 % (w/v) inoculum resulted in the highest LDPE (21.93 %) and HDPE (2.79 %) weight reduction after 30 days of incubation. FTIR analysis revealed clear structural alterations in the plastic films, confirming active biodegradation. PET and LDPE degradation showed strong statistical responses (<em>p</em> = 0.0010 and <em>p</em> = 0.0100) driven mainly by incubation time. The lack-of-fit across all models (PET: 0.7599; HDPE: 0.9281; LDPE: 0.9918) further validated model reliability. Collectively, these findings highlight the potential of <em>A. fumigatus</em> to degrade PET, HDPE, and LDPE in BODW, supporting its use as an alternative approach for future plastic biodegradation.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"33 ","pages":"Article 102534"},"PeriodicalIF":0.0,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145922141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}