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Effective simultaneous production of yeast oil and β-carotene from biodiesel-based crude glycerol in a cell-recycled continuous fermentation system producing bio-polyurethane and encapsulated β-carotene 生物柴油基粗甘油在细胞循环连续发酵系统中有效同时生产酵母油和β-胡萝卜素,生产生物聚氨酯和包封β-胡萝卜素
Q1 Environmental Science Pub Date : 2026-01-07 DOI: 10.1016/j.biteb.2026.102556
Sasitorn Khuntong , Parichat Koedprasong , Pensri Plangklang , Thidarat Samranrit , Witsanu Srila , Surachai Techaoei , Apinan Wanlapa , Kanungnid Busarakam , Atsadawut Areesirisuk
The rising demand for high-value products has sparked interest in producing microbial oils and β-carotene. Simultaneously, advances in microbial processing have accelerated the utilization of alternative renewable resources. Biodiesel-based crude glycerol (BCG) has been valorized through yeast cultivation for bio-polyurethane (BPU) and β-carotene synthesis. Under continuous fermentation, the highest glycerol consumption, biomass, yeast oil (YO), and carotene production were achieved at a dilution rate of 0.005 h−1. The cell-recycled continuous fermentation (CR-CF) strategy enhanced the volumetric productivity of yeast biomass (QX; 0.056 g/L·h), oil (QYO; 0.033 g/L·h), and β-carotene (QC; 0.207 mg/L·h) by 1.81-, 2.36-, and 1.07-fold, respectively compared to conventional CF, with 76.6% glycerol consumption efficiency. YO was successfully converted to semi-rigid BPU, with a density of 0.98 g/cm3 and 47.60% water absorption. β-Carotene extract encapsulated by spray drying with gum Arabic and maltodextrin provided high solubility, photostability, and antimicrobial activity. These results demonstrated that BCG was an effective and sustainable alternative feedstock for co-producing valuable microbial products. The CR-CF system showed promise for improving production efficiency. Additionally, the value upgrading strategy of YO and β-carotene was an attractive process for environmentally friendly and sustainable biological product formation.
对高价值产品不断增长的需求激发了人们对生产微生物油和β-胡萝卜素的兴趣。同时,微生物处理技术的进步加速了可替代再生资源的利用。以生物柴油为基础的粗甘油(BCG)为原料,通过酵母培养对生物聚氨酯(BPU)和β-胡萝卜素的合成进行了评价。在连续发酵条件下,当稀释率为0.005 h−1时,甘油消耗量、生物量、酵母油(YO)和胡萝卜素产量最高。细胞循环连续发酵(CR-CF)策略使酵母生物量(QX; 0.056 g/L·h)、油脂(QYO; 0.033 g/L·h)和β-胡萝卜素(QC; 0.207 mg/L·h)的体积生产率分别提高了1.81倍、2.36倍和1.07倍,甘油消耗效率为76.6%。YO成功转化为半刚性BPU,密度为0.98 g/cm3,吸水率为47.60%。用阿拉伯胶和麦芽糊精喷雾干燥包封的β-胡萝卜素提取物具有高溶解度、光稳定性和抗菌活性。这些结果表明,BCG是一种有效和可持续的可替代原料,可用于共同生产有价值的微生物产品。CR-CF系统有望提高生产效率。此外,YO和β-胡萝卜素的价值升级策略是形成环境友好和可持续的生物产品的一个有吸引力的过程。
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引用次数: 0
Exploration of the pathway and mechanism of phycocyanin accumulation: A review 藻蓝蛋白积累途径及机制的研究进展
Q1 Environmental Science Pub Date : 2026-01-06 DOI: 10.1016/j.biteb.2025.102535
Xiaoxuan Zhou , Zhengdong Wan , Ran Gao , Jianxiong Hao , Wei Wang , Xuechao Zheng , Dandan Zhao
Phycocyanin (PC), a bioactive pigment-protein complex found in cyanobacteria, has gained significant attention due to its diverse applications in food, pharmaceuticals, and cosmetics, particularly as a natural blue colorant and nutritional supplement in food products. This review summarizes regulatory mechanisms and enhancement strategies for PC accumulation, focusing on single/combined exogenous substances and photodynamic synergy. These interventions regulate PC synthesis mainly via energy optimization, metabolic balance, and cellular protection. Challenges like high costs and scalability remain. Future research should develop industrial by-products/agricultural waste as low-cost exogenous substances, optimize intelligent photobioreactors (PBRs), apply genetic engineering/mutagenesis to break PC synthesis limits, and integrate transcriptomics, proteomics, and metabolomics to boost industrial efficiency. This review provides a theoretical basis for PC development, supporting the sustainable bioeconomy and the advancement of the protein industry.
藻蓝蛋白(PC)是一种在蓝藻细菌中发现的具有生物活性的色素蛋白复合物,由于其在食品、药品和化妆品中的多种应用而受到广泛关注,特别是作为天然蓝色着色剂和食品中的营养补充剂。本文综述了PC积累的调控机制和增强策略,重点介绍了单一/联合外源物质和光动力协同作用。这些干预措施主要通过能量优化、代谢平衡和细胞保护来调节PC合成。高成本和可扩展性等挑战依然存在。未来的研究应将工业副产品/农业废弃物开发为低成本外源物质,优化智能光生物反应器(pbr),应用基因工程/诱变技术突破PC合成限制,并整合转录组学、蛋白质组学和代谢组学来提高工业效率。本文的研究为生物蛋白的发展、支持生物经济的可持续发展和蛋白质产业的发展提供了理论依据。
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引用次数: 0
Thermally repairable and antimicrobial chitosan-gelatin films with natural wax and silver nanoparticles 热修复和抗菌壳聚糖明胶膜与天然蜡和银纳米颗粒
Q1 Environmental Science Pub Date : 2026-01-06 DOI: 10.1016/j.biteb.2025.102533
Neda Aliabbasi , Zahra Emam-Djomeh , Hassan Rezaeinia
This research article aimed to develop biodegradable chitosan-gelatin composite films reinforced with natural wax and silver nanoparticles (AgNPs), indicating considerable mechanical, barrier, antimicrobial, and heat-induced sealing. The films showed a great tensile strength exceeding 58 MPa, significantly enhanced water contact angles up to 117.5°, revealing superior hydrophobicity, and a thermal healing efficiency reaching 82.4 % after thermal annealing. The integration of AgNPs enhanced not only the antimicrobial activity against Gram-positive and Gram-negative bacteria but also the mechanical performance and water vapor barrier properties of the films. The thermally triggered repair capability of the films stems from temperature-driven translocation of incorporated natural wax to superficial regions, which effectively restores both hydrophobicity and mechanical integrity following induced damage. These results propose that the developed composite films are promising candidates for sustainable packaging applications as environmentally friendly alternatives to synthetic plastics.
本研究旨在开发以天然蜡和纳米银(AgNPs)增强的可生物降解壳聚糖-明胶复合薄膜,具有良好的机械、屏障、抗菌和热致密封性能。薄膜的拉伸强度超过58 MPa,水接触角显著增强,达到117.5°,表现出优异的疏水性,热处理后的热愈合效率达到82.4%。AgNPs的整合不仅提高了膜的抗革兰氏阳性和革兰氏阴性菌的抗菌活性,而且提高了膜的力学性能和水蒸气阻隔性能。膜的热触发修复能力源于加入的天然蜡在温度驱动下向表面区域的移位,这有效地恢复了诱导损伤后的疏水性和机械完整性。这些结果表明,所开发的复合薄膜作为合成塑料的环保替代品,是可持续包装应用的有希望的候选者。
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引用次数: 0
Biochar-reinforced biodegradable mulching films: Toward climate-resilient and sustainable agriculture 生物炭增强可生物降解地膜:走向气候适应性和可持续农业
Q1 Environmental Science Pub Date : 2026-01-06 DOI: 10.1016/j.biteb.2025.102525
Sefiu Olaitan Amusat , Simiso Dube , Jabulani I. Mnyango , Shanganyane P. Hlangothi , Bienvenu Gael Fouda Mbanga , Seyisi Thulethu , Sabelo Mhlanga , Zikhona Tywabi-Ngeva
The reinforcement of biodegradable mulching films with biochar represents a promising strategy to enhance the performance, sustainability, and environmental compatibility of water and nutrient management in agricultural practices. To guarantee food security, sustainable water management techniques must be developed. As water scarcity intensifies under changing climate conditions, there is an urgent need for environmentally responsible practices that enhance soil–water dynamics while supporting crop productivity. Recently, biodegradable mulching films have emerged as a promising, eco-friendly alternative to traditional plastic mulches. However, their mechanical strength, degradation behaviors, and long-term field stability remain key limitations. In this review, we critically examine and evaluate the potential of biochar-reinforced biodegradable mulching films as a next-generation solution for sustainable agriculture. Various types, properties, and mechanisms of biodegradable mulching films were discussed in this review. We also highlighted the prospect of application of biochar-reinforced biodegradable mulching films, highlighting their potential to address pressing challenges in climate-resilient and sustainable agriculture. We provided the synergistic advantages of biochar in enhancing the mechanical characteristics, thermal stability, water retention, and nutrient management of mulching films, while also contributing to carbon sequestration and soil health. This review emphasizes a novel pathway to developing cutting-edge mulching technologies that can more effectively promote climate-resilient food production by bridging knowledge across soil science, materials engineering, and climate-smart agriculture.
用生物炭加固可生物降解地膜是一种很有前途的策略,可以提高农业实践中水和养分管理的性能、可持续性和环境相容性。为了保证粮食安全,必须开发可持续的水管理技术。在气候条件不断变化的情况下,水资源短缺加剧,迫切需要采取对环境负责的做法,加强土壤-水动态,同时支持作物生产力。最近,可生物降解地膜已经成为传统塑料地膜的一种有前途的、环保的替代品。然而,它们的机械强度、降解行为和长期现场稳定性仍然是主要的限制。在这篇综述中,我们批判性地研究和评估了生物炭增强可生物降解地膜作为可持续农业的下一代解决方案的潜力。综述了生物可降解地膜的种类、性能和作用机理。我们还强调了生物炭增强可生物降解地膜的应用前景,强调了它们在应对气候适应型和可持续农业方面的紧迫挑战方面的潜力。我们提供了生物炭在提高地膜力学特性、热稳定性、保水和养分管理方面的协同优势,同时也有助于固碳和土壤健康。这篇综述强调了开发尖端覆盖技术的新途径,通过将土壤科学、材料工程和气候智能型农业之间的知识联系起来,可以更有效地促进气候适应型粮食生产。
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引用次数: 0
Hydrodynamic cavitation-assisted intensified extraction of pectin from sweet lime peels using deep eutectic solvent 流体动力空化辅助深共熔溶剂强化提取甜石灰果皮中的果胶
Q1 Environmental Science Pub Date : 2026-01-06 DOI: 10.1016/j.biteb.2025.102517
Birupakshya Mishra , Sanjana Pal , U. Jayakrishnan , Chalicheemala Srinath , Subhankar Roy , Siddhartha Moulik , Alka Kumari
The present work demonstrates the technological advancement in the resource recovery approaches to extract a high-value product, pectin, from sweet lime peel (SLP) using Choline chloride: Citric acid deep eutectic solvent (DES) and a novel rotating hydrodynamic cavitation (RHC) reactor. The present work employs a low-carbon circular bio-economy by combining RHC technology for the valorization of SLP through pectin extraction. Numerical simulations using computational fluid dynamics revealed the optimum geometrical parameters for the cavitation generation unit to be of semi-circular shape, 9 mm depth, 10 mm diameter with 504 units. Box–Behnken Design of pectin extraction using numerically optimized RHC indicated that time of 32.8 min, rotational speed of 1800 rpm, solid: liquid ratio of 1:30, at fixed DES: water ratio of 1:6, yielded maximum pectin of 32.89 % with 88.24 % degree of esterification. The Analysis of Variance assessment indicated the influence of process parameters in the order rotational speed>extraction time > solid-to-liquid ratio. The physicochemical characterization of the RHC-extracted pectin observed similar morphology and structure to that of commercial pectin. The process developed ensured higher pectin yield having high thermal stability but resulted in possible truncation of pectin chains into oligomers with molecular weight 60.04–901.10 kDa. The scale-up study has demonstrated the feasibility of an industrial scale process and its cost-effective aspects with a significant return on investment within a year.
本研究展示了利用氯化胆碱-柠檬酸深共晶溶剂(DES)和新型旋转流体动力空化反应器(RHC)从甜石灰果皮(SLP)中提取高价值产品果胶的资源回收方法的技术进展。本研究采用低碳循环生物经济,结合RHC技术,通过果胶提取实现SLP的增值。采用计算流体力学方法进行了数值模拟,结果表明,空化产生单元的最佳几何参数为半圆形、深度9 mm、直径10 mm,共504个单元。数值优化的RHC法提取果胶的Box-Behnken设计表明,在固定的DES与水比为1:6的条件下,时间为32.8 min,转速为1800 rpm,料液比为1:30,果胶收率为32.89%,酯化度为88.24%。方差分析表明,工艺参数对萃取效果的影响依次为:转速、萃取时间、料液比。rhc提取的果胶的物理化学性质与商品果胶的形态和结构相似。所开发的工艺保证了较高的果胶收率和较高的热稳定性,但可能导致果胶链截断成分子量为60.04-901.10 kDa的低聚物。放大研究证明了工业规模工艺的可行性及其成本效益方面,在一年内投资回报显著。
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引用次数: 0
Biochar integration in biomethanation process: Addressing limitations and unlocking performance gains 生物甲烷化过程中的生物炭整合:解决限制并解锁性能增益
Q1 Environmental Science Pub Date : 2026-01-06 DOI: 10.1016/j.biteb.2025.102512
Jyoti Moni Devi , Biplab Kumar Debnath , Rajat Subhra Das , Biplab Das
Anaerobic digestion (AD) is a well-established biological process used to convert and treat complex substrates. However, its widespread implementation is constrained by several challenges. The use of additives such as biochar (BC) is proposed to address these challenges. This review summarizes the applications of BC in AD, including parent materials for BC-assisted AD systems, substrates used in AD, BC's function, including parent materials for BC-assisted AD systems, BC's role in AD, engineered BC-assisted AD approaches, and its application in biogas purification. Additionally, to address the expansion of BC incorporation, a strengths-weaknesses-opportunities-threats (SWOT) analysis is conducted. Despite the numerous benefits associated with BC, its cost-effectiveness compared to energy gains is vital. A key issue identified is the lack of large-scale studies. Limited studies that have examined the long-term regulatory effects of BC in full-scale reactors. Future research can concentrate on optimizing BC performance and refining production methods to enhance cost-effectiveness. Effective policies can incentivize the use of biogas and BC expertise, endorsing their broader application.
厌氧消化(AD)是一种成熟的生物过程,用于转化和处理复杂的底物。然而,它的广泛实施受到一些挑战的限制。建议使用诸如生物炭(BC)之类的添加剂来解决这些挑战。本文综述了BC在AD中的应用,包括BC辅助AD系统的母体材料、AD中使用的底物、BC的功能(包括BC辅助AD系统的母体材料)、BC在AD中的作用、工程BC辅助AD方法及其在沼气净化中的应用。此外,为了解决BC公司的扩张,进行了优势-劣势-机会-威胁(SWOT)分析。尽管与BC相关的诸多好处,但与能源收益相比,其成本效益至关重要。确定的一个关键问题是缺乏大规模研究。有限的研究检查了BC在全尺寸反应器中的长期调节作用。未来的研究可以集中在优化BC性能和改进生产方法上,以提高成本效益。有效的政策可以激励沼气和卑诗技术的使用,支持其更广泛的应用。
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引用次数: 0
Hidden engineers of decomposition: Invertebrate communities and their association with composting parameters in vegetable waste pile 分解的隐藏工程师:无脊椎动物群落及其与蔬菜废物堆堆肥参数的关系
Q1 Environmental Science Pub Date : 2026-01-05 DOI: 10.1016/j.biteb.2025.102516
Sujata Phurailatpam , Meena Khwairakpam
The significance of microorganisms in composting is well-established, but the ecological role of invertebrates has received comparatively less attention. The present study addresses this gap by examining the abundance and diversity of invertebrates and their correlations with physicochemical and biological parameters during pile composting. Composting was carried out using a blended substrate of vegetable waste, cow dung, and sawdust. The invertebrates from the waste biomass were extracted using a modified Berlese Tullgren Funnel. During the study, a total of 11,344 mesofauna and macrofauna individuals were collected and identified during the pile composting. Springtails dominated the community, followed by mites and black soldier fly larvae, underscoring the central role of these taxa in waste decomposition. Diversity analyses using the Shannon–Wiener and Simpson indices revealed clear associations between community structure and compost dynamics. In addition, the physicochemical and biological parameters of the waste biomass agreed with the variation of abundance of the invertebrates during the process. The highest abundance of invertebrates was observed within the temperature range of 33.5 °C to 37.3 °C, and corresponding moisture content from 35.24 % to 38.01 % conditions favourable for both decomposition and faunal activity. These findings highlight invertebrates as active drivers of compost transformation, bridging biological activity with physicochemical processes. The study provides a foundation for the strategic utilization of key invertebrate taxa to enhance feedstock decomposition and improve composting efficiency.
微生物在堆肥中的重要性已得到公认,但无脊椎动物的生态作用却相对较少受到关注。本研究通过研究堆堆肥过程中无脊椎动物的丰度和多样性及其与物理化学和生物参数的相关性来解决这一差距。堆肥是用蔬菜废料、牛粪和锯末混合的基质进行的。利用改进的Berlese Tullgren漏斗从废弃生物质中提取无脊椎动物。在研究过程中,共收集和鉴定了11344个中、大型动物个体。弹尾虫在群落中占主导地位,其次是螨虫和黑兵蝇幼虫,强调了这些分类群在废物分解中的核心作用。利用Shannon-Wiener指数和Simpson指数进行多样性分析,发现群落结构与堆肥动态之间存在明显的相关性。此外,废弃生物质的理化生物学参数与无脊椎动物丰度的变化过程一致。无脊椎动物丰度最高的温度范围为33.5℃~ 37.3℃,相应的水分含量为35.24% ~ 38.01%,有利于分解和动物活动。这些发现强调了无脊椎动物作为堆肥转化的积极驱动力,将生物活性与物理化学过程联系起来。该研究可为重点无脊椎动物类群的战略性利用提供基础,以促进原料分解和提高堆肥效率。
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引用次数: 0
The shelf life of Mosaccha tempeh under different packaging conditions and storage temperatures 不同包装条件和贮存温度下摩萨茶豆豉的保质期
Q1 Environmental Science Pub Date : 2026-01-05 DOI: 10.1016/j.biteb.2026.102547
Samsul Rizal , Maria Erna Kustyawati , Novita Herdiana , Dewi Sartika , Fairuzsita Naura Amalia Syifani , Honi Aisya , Dita Artha Ristiani , Ayu Dian Pratiwi Permatahati
Mosaccha tempeh is a soybean-based fermented product produced using a mixed-culture inoculum of Rhizopus oligosporus and Saccharomyces cerevisiae, which enhances its nutritional value and sensory properties compared with conventional fermentation. Despite these benefits, its shelf life remains limited due to rapid microbial activity at ambient temperatures. This study evaluated the effects of vacuum and non-vacuum packaging under room and cold storage conditions on the quality and shelf life of Mosaccha tempeh. Tempeh was stored for four days at room temperature and seven days under refrigeration, with daily measurements of water content, hardness, soluble protein, and sensory attributes. Data were analyzed using ANOVA followed by the Honest Significant Difference (HSD) test at the 5 % significance level. Under room-temperature storage, non-vacuum packaging maintained acceptable sensory quality for up to two days, with corresponding values of 65.37 % water content, 45.80 % soluble protein, and 580.08 gf hardness, and the product remained consumable through day three. Under cold storage, vacuum packaging preserved quality more effectively, maintaining sensory acceptability for up to four days, supported by 61.52 % water content, 41.82 % soluble protein, and 469.25 gf hardness, with acceptability extending to day six. These findings provide practical guidance for producers of Mosaccha tempeh in selecting suitable packaging and storage conditions and for consumers to maximize product quality and shelf life. Overall, this study demonstrates that Mosaccha tempeh is a viable alternative fermented product with competitive shelf-life characteristics, supporting its potential for wider distribution and value-added applications.
Mosaccha tempeh是一种以大豆为基础的发酵产品,采用少孢根霉和酿酒酵母混合培养接种,与传统发酵相比,提高了其营养价值和感官特性。尽管有这些好处,但由于微生物在环境温度下的快速活动,其保质期仍然有限。本研究考察了常温和冷藏条件下真空和非真空包装对摩萨茶豆豉品质和保质期的影响。豆豉在室温下保存4天,在冷藏下保存7天,每天测量其含水量、硬度、可溶性蛋白质和感官属性。数据分析采用方差分析,然后在5%显著性水平上进行诚实显著差异(HSD)检验。在室温下,非真空包装在2天内保持了良好的感官质量,相应的水分含量为65.37%,可溶性蛋白含量为45.80%,硬度为580.08 gf,产品在第三天仍然是可消费的。在冷藏条件下,真空包装能更有效地保存品质,在61.52%的含水量、41.82%的可溶性蛋白和469.25 gf的硬度的支持下,其感官可接受度可维持4天,可接受度可延长至第6天。这些研究结果为摩萨卡豆豉生产商选择合适的包装和储存条件以及消费者最大限度地提高产品质量和保质期提供了实践指导。总的来说,这项研究表明,摩萨茶豆豉是一种可行的替代发酵产品,具有具有竞争力的保质期特征,支持其更广泛的分销和增值应用潜力。
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引用次数: 0
Role of hexa-aquo copper complex in microwave-assisted catalytic conversion of pine needles to glucose and 5-hydroxymethylfurfural in the presence of protic ionic liquid: Experimental and kinetic study 在质子离子液体存在下,六水铜配合物在松针微波催化转化为葡萄糖和5-羟甲基糠醛中的作用:实验和动力学研究
Q1 Environmental Science Pub Date : 2026-01-05 DOI: 10.1016/j.biteb.2025.102530
Subhrajit Roy, Bhakti Godbole, Prakash Biswas
This study investigated the role of the hexaaquo complex ([Cu(H2O)6]2+) in the microwave-assisted conversion of lignocellulosic pine needles (Pinus roxburghii) to 5-hydroxymethylfurfural (HMF) using triethylammonium hydrogen sulfate as a protic ionic liquid (PIL). In the reaction medium, cellulose was hydrolyzed into monomeric sugars, such as glucose and fructose, in the presence of PIL–water and copper chloride catalysts. The polar [Cu(H2O)6]2+ complex interacted with cellulose, facilitating bond cleavage and subsequent dehydration reactions. Under microwave irradiation, in the presence of CuCl2 catalyst in the ionic liquid medium, glucose dehydrated to HMF via the elimination of water molecules. Acid hydrolysis, facilitated by the formation of in-situ HCl in the PIL, helped to break the β-1,4-glycosidic bonds in cellulose. Additionally, the presence of free chloride ions in the PIL–CuCl2 system enhanced glucose-to-fructose isomerization, a key reaction step in maximizing HMF yield. A comparatively moderate HMF yield of 16 % was achieved under these conditions, while the absence of free chloride ions led to a lower yield (∼1.8 %), highlighting the regulatory role of chloride ion availability. The study emphasized the dual catalytic role of the [Cu(H2O)6]2+ complex in both hydrolysis and dehydration pathways. A detailed kinetic modelling study was conducted to evaluate rate constants and activation energies, providing insights into the influence of the [Cu(H2O)6]2+ complex and microwave energy on reaction efficiency and selectivity.
研究了六水络合物([Cu(H2O)6]2+)在微波辅助下以三乙基硫酸氢铵作为质子离子液体(PIL)将木质纤维素松针(Pinus roxburghii)转化为5-羟甲基糠醛(HMF)中的作用。在反应介质中,纤维素被水解成单体糖,如葡萄糖和果糖,在氧化锌水和氯化铜催化剂的存在下。极性[Cu(H2O)6]2+配合物与纤维素相互作用,促进键断裂和随后的脱水反应。在微波照射下,在离子液体介质中有CuCl2催化剂存在的情况下,葡萄糖通过消除水分子脱水成HMF。在PIL中原位HCl的形成促进了酸水解,有助于破坏纤维素中的β-1,4-糖苷键。此外,在PIL-CuCl2体系中自由氯离子的存在增强了葡萄糖到果糖的异构化,这是最大化HMF产量的关键反应步骤。在这些条件下,HMF的产率相对适中,为16%,而缺乏自由氯离子导致产率较低(~ 1.8%),突出氯离子可用性的调节作用。研究强调了[Cu(H2O)6]2+配合物在水解和脱水两种途径中的双重催化作用。通过详细的动力学建模研究,评估了反应速率常数和活化能,深入了解了[Cu(H2O)6]2+配合物和微波能量对反应效率和选择性的影响。
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引用次数: 0
Biodegradation of PET and PE by tropical landfill derived Aspergillus fumigatus in modified BOD enriched medium 热带垃圾填埋场衍生烟曲霉在改性BOD富集培养基中降解PET和PE的研究
Q1 Environmental Science Pub Date : 2026-01-05 DOI: 10.1016/j.biteb.2025.102534
Priya Mohan , Fauziah Shahul Hamid
Landfilled plastics serve as an untapped carbon reservoir, fostering selective conditions that enable microbial communities to compete and metabolize the available polymer-derived carbon. This study aims to evaluate the biodegradation potential of fungal strains isolated from Jeram Sanitary Landfill on PET, HDPE, and LDPE plastics. A total of eight fungal strains were isolated from plastic waste and tested in two liquid media, namely modified Biochemical Oxygen Demand Dilution Water (BODW) and Bushnell Haas media to determine the optimal media for degradation. Among the isolates, Aspergillus fumigatus (FI 5) exhibited the highest degradation efficiency, achieving 1.8 % and 2.0 % in BODW and Bushnell Haas media, respectively. A cost-benefit analysis identified BODW media as the more economical media, warranting further optimization. Degradation efficiency was significantly influenced by fungal inoculum concentration, where 1 % (w/v) inoculum yielded the highest PET weight loss (1.49 %), while a 5 % (w/v) inoculum resulted in the highest LDPE (21.93 %) and HDPE (2.79 %) weight reduction after 30 days of incubation. FTIR analysis revealed clear structural alterations in the plastic films, confirming active biodegradation. PET and LDPE degradation showed strong statistical responses (p = 0.0010 and p = 0.0100) driven mainly by incubation time. The lack-of-fit across all models (PET: 0.7599; HDPE: 0.9281; LDPE: 0.9918) further validated model reliability. Collectively, these findings highlight the potential of A. fumigatus to degrade PET, HDPE, and LDPE in BODW, supporting its use as an alternative approach for future plastic biodegradation.
垃圾填埋的塑料作为一个未开发的碳库,培育了选择性条件,使微生物群落能够竞争和代谢可用的聚合物衍生碳。本研究旨在评价从Jeram卫生填埋场分离的真菌菌株对PET、HDPE和LDPE塑料的生物降解潜力。从塑料废弃物中分离出8株真菌,分别在改性生化需氧量稀释水(BODW)和Bushnell - Haas培养基两种液体培养基中进行试验,以确定最佳降解培养基。其中,烟曲霉(Aspergillus fumigatus, FI 5)对BODW和Bushnell Haas培养基的降解效率最高,分别为1.8%和2.0%。成本效益分析表明,BODW介质是更经济的介质,值得进一步优化。真菌接种量对降解效率有显著影响,接种量为1% (w/v)时PET失重率最高(1.49%),接种量为5% (w/v)时LDPE和HDPE失重率最高(21.93%)和2.79%。FTIR分析显示塑料薄膜的结构发生了明显的变化,证实了活性生物降解。PET和LDPE降解表现出较强的统计学响应(p = 0.0010和p = 0.0100),主要受孵育时间的影响。所有模型的拟合缺失(PET: 0.7599; HDPE: 0.9281; LDPE: 0.9918)进一步验证了模型的可靠性。总的来说,这些发现突出了烟曲霉在BODW中降解PET、HDPE和LDPE的潜力,支持其作为未来塑料生物降解的替代方法。
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Bioresource Technology Reports
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