Bioresource Technology Reports最新文献_第6页

Advancing urban food sustainability: Biotechnology and IoT synergies in vertical greenhouses 推进城市食品可持续性：垂直温室中的生物技术和物联网协同效应

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-01-05 DOI: 10.1016/j.biteb.2026.102548

Pooja Sharma , Nitika Thakur

Vertical greenhouse farming represents a transformative approach to urban sustainable agriculture, integrating controlled environment systems and advanced technologies to address the challenges of food security, resource scarcity and environmental degradation. This review evaluates evidence demonstrating how the incorporation of microbial biotechnology, Internet of Things (IoT) and Artificial Intelligence (AI) enhances nutrient uptake, bolsters disease resistance and improves stress tolerance in vertical greenhouses and soil-less cultivation systems. This review reveals that the progress in IoT and AI-driven automation has further facilitated precise regulation of microclimatic conditions, irrigation and nutrient delivery, thereby minimizing input waste and operational costs while ensuring consistently high yields. According to the recent literature, smart vertical greenhouses demonstrated 20–35 % improvements in crop resilience under simulated stress conditions, up to 40 % increase in crop yield, alongside 30 % reductions in water and fertilizer inputs. Emerging research underscores the significance of developing customized microbial inoculants, genome-edited crops with compact growth habits and renewable energy solutions to augment the viability and scalability of these systems. The findings identify critical gaps in field validation and regulatory standardization, emphasizing the importance of interdisciplinary collaboration, robust data infrastructures and supportive policies to fully realize the potential of sustainable, resilient vertical farming. Subsequently, the integration of biotechnology and digital agriculture offers a promising pathway to strengthen resilient, efficient and environmentally responsible food production against global crises in an increasingly urbanized world.

垂直温室农业代表了城市可持续农业的一种变革性方法，将受控环境系统和先进技术结合起来，以应对粮食安全、资源稀缺和环境退化的挑战。本综述评估了在垂直温室和无土栽培系统中，微生物生物技术、物联网（IoT）和人工智能（AI）的结合如何增强养分吸收、增强抗病性和提高抗逆性的证据。这一综述表明，物联网和人工智能驱动的自动化技术的进步进一步促进了对小气候条件、灌溉和养分输送的精确调节，从而最大限度地减少了投入浪费和运营成本，同时确保了持续的高产量。根据最近的文献，智能垂直温室在模拟压力条件下的作物抗逆性提高了20 - 35%，作物产量增加了40%，同时减少了30%的水和肥料投入。新兴研究强调了开发定制微生物接种剂、具有紧凑生长习惯的基因组编辑作物和可再生能源解决方案的重要性，以增强这些系统的可行性和可扩展性。研究结果指出了现场验证和监管标准化方面的关键差距，强调了跨学科合作、强大的数据基础设施和支持性政策的重要性，以充分发挥可持续、有弹性的垂直农业的潜力。因此，在日益城市化的世界中，生物技术与数字农业的结合为加强有韧性、高效和对环境负责的粮食生产以应对全球危机提供了一条有希望的途径。

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引用次数: 0

Integrated hydroponics systems with anaerobic biogas slurry: Analysis of nitrified bio slurry, plant growth performance, and human health risk assessment 厌氧沼液一体化水培系统：硝化沼液分析、植物生长性能和人体健康风险评估

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-01-05 DOI: 10.1016/j.biteb.2025.102538

Tarek Mahrous , Ping Ai , Ali Abd-Elkader , Aibin Hu , Mahdy Elsayed

Integration of biogas digestate into hydroponics promotes circular agriculture but faces nutrient imbalances and phytotoxicity. This study evaluated the use of chicken digestate in hydroponic perilla frutescens production at 10 %, 15 %, and 20 % (diluted with 1/4stregth Hoagland solution). Besides 20 % digestate under real-time nitrification, comparing water and 1/4 strength Hoagland solution as diluents. Lower concentrations (10–15 %) enhanced biomass, while 20 % digestate reduced nutrient uptake and inhibited growth. Biological nitrification of 20 % converts 48.7 % ammonia to nitrate, mitigating ammonium toxicity and improving nutrient bioavailability. This nitrified digestate significantly improved seedling vigor, increasing photosynthesis rate by 53 %, relative growth rate by 20.4 %, and seedling index by 139.3 % compared to chemical fertilizer. Risk (non-carcinogenic and carcinogenic) assessments indicated no human health hazards over lifetime. The findings demonstrate that nitrification pretreatment and optimized dilution are crucial for safely and efficiently integrating digestate in hydroponics, supporting circular nutrient management and sustainable plant production.

将沼液整合到水培中促进循环农业，但面临营养不平衡和植物毒性。本研究评估了10%、15%和20%（用1/4强度的Hoagland溶液稀释）的鸡消化液在水培紫苏生产中的应用。此外，20%的消化液在实时硝化下，比较水和1/4浓度的霍格兰溶液作为稀释剂。较低浓度（10 - 15%）增加了生物量，而20%的消化物降低了养分吸收并抑制了生长。20%的生物硝化作用将48.7%的氨转化为硝酸盐，减轻氨的毒性，提高养分的生物利用度。与化肥相比，硝化消化液显著提高了幼苗活力，光合速率提高了53%，相对生长率提高了20.4%，幼苗指数提高了139.3%。风险（非致癌性和致癌性）评估表明，在一生中对人类健康没有危害。研究结果表明，硝化预处理和优化稀释对于安全有效地整合水培消化液，支持循环养分管理和植物可持续生产至关重要。

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引用次数: 0

Integrated valorization of domestic wastewater for phycoremediation and polyhydroxybutyrate (PHB) production by Synechocystis salina M8 盐渍胞囊藻M8合成聚羟基丁酸盐（PHB）及藻修复的综合酸化研究

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-01-05 DOI: 10.1016/j.biteb.2025.102521

Do Thi Cam Van , Nguyen Thi Phuong Dung , Bui Thi Thu Uyen , Tran Dang Thuan , Le Truong Giang

This study evaluates the potential of the marine cyanobacterium Synechocystis salina M8 for integrated wastewater remediation and polyhydroxybutyrate (PHB) bioplastic production. The effects of key physicochemical parameters, including C/N/P ratio, pH, light intensity, and wastewater sterilization, were systematically optimized under phototrophic and mixotrophic conditions. Optimal growth occurred at a C/N/P ratio of 70:7:1, pH 7, and 13,500 lx, yielding a biomass concentration of 3.4 g L⁻¹ and PHB content of 50.2 % of dry cell weight (DCW). The strain achieved nutrient removal efficiencies of 99.6 % COD, 99.6 % BOD, 90.3 % total nitrogen, and 95.9 % total phosphorus in 30 L-scaled photobioreactors under outdoor cultivation, demonstrating strong phycoremediation capacity. Structural analyses of the purified PHB by FT-IR, ¹H NMR, and ¹³C NMR confirmed functional equivalence with other cyanobacteria-derived PHB, while gel permeation chromatography revealed a high molecular weight (Mw = 856.9 kg mol⁻¹) and broad polydispersity index (Mw/Mn = 4.34). Compared with previously reported Synechocystis strains in pilot cultures, S. salina M8 exhibited remarkably higher PHB productivity in real wastewater (0.14 g L⁻¹ d⁻¹). These results demonstrate the dual environmental and biotechnological potential of S. salina M8 for sustainable bioplastic production and nutrient recovery within a circular bioeconomy framework.

本研究评估了海洋蓝藻藻盐合囊藻M8在污水综合修复和聚羟基丁酸生物塑料生产中的潜力。在光养和混合营养条件下，系统优化了C/N/P比、pH、光照强度和废水灭菌等关键理化参数的影响。在C/N/P比为70:7:1、pH为7和13500 lx的条件下生长最佳，生物量浓度为3.4 g L−1，PHB含量为干细胞重量（DCW）的50.2%。该菌株在室外培养的30个l级光生物反应器中，COD去除率为99.6%，BOD去除率为99.6%，总氮去除率为90.3%，总磷去除率为95.9%，具有较强的藻修复能力。通过FT-IR， 1H NMR和13C NMR对纯化的PHB进行结构分析，证实其与其他蓝藻衍生的PHB功能等同，凝胶渗透色谱分析显示其分子量高（Mw = 856.9 kg mol - 1），多分散指数广（Mw/Mn = 4.34）。与先前报道的中试培养的联胞菌菌株相比，S. salina M8在实际废水中表现出显著更高的PHB生产力（0.14 g L−1 d−1）。这些结果表明，在循环生物经济框架下，S. salina M8具有可持续生物塑料生产和养分回收的双重环境和生物技术潜力。

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引用次数: 0

Bioprospecting of lithophytic microalgae for photoprotective compounds against UV-B radiation: A review 岩生微藻抗UV-B辐射光防护化合物的生物勘探进展

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-01-05 DOI: 10.1016/j.biteb.2025.102527

Vigneswaran Dharmalingam, Antony Prakash Rejoy Patrick, Vigneshkumar Venkatesan, Nagaraj Subramani

Microalgae are a diverse group of photosynthetic microorganisms that represent a largely untapped source of bioactive compounds. Microalgae demonstrate significant potential for biotechnological innovation and sustainable development. Their adaptability enables them to colonize diverse habitats, in particularly unusual habitats such as monuments, caves, and facades which are underexplored ecological niches and exhibit specialized adaptive mechanisms against environmental stressors, particularly Ultraviolet-B (UV-B) radiation. Certain lithophytic microalgae species of Chroococcidiopsis sp., Nostoc sp., Scytonema sp., and Klebsormidium sp. are triggered by the prolonged exposure to solar radiation, which helps in synthesis of unique potent photoprotective compounds such as Mycosporine-like amino acids (shinorine, porphyra-334), Scytonemin, Carotenoids, and Phenolic derivatives, which play a crucial role in shielding cells from intense UV-B radiation. These compounds correspond to cosmeceutical applications, especially for mitigating UV-induced oxidative stress, and serve as eco-friendly alternatives to synthetic UV filters. They function as natural sunscreens by absorbing or dissipating harmful UV-B rays by scavenging reactive oxygen species (ROS). Despite their ecological and biochemical significance, the photoprotective properties of lithophytic microalgae are less studied than those of episammic, epilithic and phytoplankton, and other habitats. This study investigates the survival strategies of lithophytic microalgae in extreme habitats and their potential as sources of UV-B protective compounds. Identification of these novel metabolites may facilitate the development of sustainable sunscreens, pharmaceuticals, and cosmetics, thereby contributing to human health. Future research should focus on genomic, metabolomic, and advanced biotechnological approaches to determine the full potential of microalgae as a contributor in sustainable environmental and industrial solutions.

微藻是一种多样化的光合微生物，代表了一种尚未开发的生物活性化合物来源。微藻在生物技术创新和可持续发展方面显示出巨大潜力。它们的适应性使它们能够在不同的栖息地定居，特别是在不寻常的栖息地，如纪念碑、洞穴和立面，这些栖息地是未被开发的生态位，并表现出针对环境压力的特殊适应机制，特别是紫外线b （UV-B）辐射。某些石生微藻，如Chroococcidiopsis sp.、Nostoc sp.、Scytonema sp.和klebsormiddium sp.被长期暴露在太阳辐射下触发，这有助于合成独特的强效光保护化合物，如真菌素类氨基酸（shinorine, porphyra-334）、Scytonemin、类胡萝卜素和酚类衍生物，它们在屏蔽细胞强烈的UV-B辐射中起着至关重要的作用。这些化合物对应于药妆应用，特别是减轻紫外线诱导的氧化应激，并作为合成紫外线过滤器的环保替代品。它们通过清除活性氧（ROS）吸收或消散有害的UV-B射线，起到天然防晒霜的作用。尽管石生微藻具有重要的生态和生化意义，但其光保护特性的研究却少于寄生、寄生和浮游植物等生境。本研究探讨了石生微藻在极端生境中的生存策略及其作为UV-B保护化合物来源的潜力。鉴定这些新的代谢物可能促进可持续防晒霜、药品和化妆品的开发，从而有助于人类健康。未来的研究应集中在基因组学、代谢组学和先进的生物技术方法上，以确定微藻作为可持续环境和工业解决方案的全部潜力。

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引用次数: 0

Next-generation strategies for rhamnolipid biosurfactant production: Metabolic optimization and sustainable process development 下一代鼠李糖脂生物表面活性剂生产策略：代谢优化和可持续工艺开发

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-01-05 DOI: 10.1016/j.biteb.2025.102528

Yashika Raheja , Satish Kumar Ainala , Tayyab Islam , Pallavi Gupta , Ajay Kumar , Vivek Kumar Gaur , Janmejai kumar Srivastava

Rhamnolipids (RLs) are biodegradable glycolipid surfactants with outstanding emulsifying, foaming and antimicrobial properties, making them attractive alternatives to petrochemical-derived surfactants in cosmetics, agrochemicals and enhanced oil recovery. Despite this potential, industrial adoption is hampered by the reliance on the native producer strains such as Pseudomonas aeruginosa, which typically produces less than 15 g/L. This review surveys recent strategies to improve rhamnolipid titers, rates and yields (TRY). The well knitted metabolic network underpinning RL biosynthesis, chassis-engineering approaches such as eliminating competing pathways, enhancing cofactor regeneration systems, and optimizing export mechanisms to redirect flux toward product formation has been highlighted. Advances in heterologous production platforms, limitations and remaining research gaps involved with the current system have been examined in detail. Further, the review provides the comprehensive analysis on process-scale demonstrations, market potential and identifying key techno-economic barriers. Finally, a complete roadmap on future directions involving integrating systems biology, bioprocess development and downstream-processing innovations to enable cost-competitive, large-scale rhamnolipid manufacture has been proposed.

鼠李糖脂（RLs）是一种可生物降解的糖脂表面活性剂，具有优异的乳化、发泡和抗菌性能，是化妆品、农用化学品和提高石油化工衍生表面活性剂的有吸引力的替代品。尽管有这种潜力，但由于依赖铜绿假单胞菌等本地生产菌株，工业应用受到阻碍，其产量通常低于15克/升。本文综述了提高鼠李糖脂滴度、率和产率（TRY）的最新策略。强调了支撑RL生物合成的良好编织代谢网络、消除竞争途径、增强辅因子再生系统和优化出口机制等底盘工程方法，以将通量重定向到产品形成。详细审查了异种生产平台的进展，当前系统所涉及的局限性和剩余的研究差距。此外，审查提供了对工艺规模示范、市场潜力和确定关键技术经济障碍的全面分析。最后，提出了一个完整的未来发展路线图，包括整合系统生物学、生物工艺开发和下游加工创新，以实现具有成本竞争力的大规模鼠李糖脂生产。

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引用次数: 0

Keratin-derived bio-adsorbents for water remediation: Current and future trends 用于水修复的角蛋白生物吸附剂：当前和未来趋势

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-01-04 DOI: 10.1016/j.biteb.2025.102508

Muhammad Zubair , Zahid Rauf , Aman Ullah

Keratin-derived bio-adsorbents have become a highly effective solution for water remediation applications, owing to their sustainable and environmentally friendly properties. Keratin, which is naturally abundant, exhibits unique physicochemical properties and is environmentally benign. It can be extracted from many biomass sources such as feathers, hooves, wool, and hair, and is characterized by a fibrous protein structure rich in functional groups such as disulfide, amino, and carboxyl groups. These attributes enable the effective binding of metal ions and organic pollutants simultaneously from the polluted water. The review highlights recent progress in keratin-based bio-adsorbents adsorbing both metal cations and oxyanions such as Pb²⁺, Ni²⁺, Co²⁺, Zn²⁺, Cr³⁺/CrO₄²⁻, Cd²⁺, AsO₃³⁻/AsO₄³⁻, and SeO₃²⁻/SeO₄²⁻, alongside organic pollutants like methylene blue, reactive blue 19, crystal violet, methyl orange, and phenolic compounds for sustainable water purification. The review also examines chemical modifications and composite materials that enhance keratin's adsorption capacity, selectivity, and regeneration potential. Additionally, it identifies significant research gaps, such as limited scalability and challenges in regeneration and reuse. Ion exchange, complexation, electrostatic interactions, and hydrogen bonding are identified as crucial mechanisms in the adsorption processes of keratin-based materials. Future research should focus on developing innovative keratin composites, employing environmentally friendly processing methods, creating mechanistic models, and conducting large-scale testing to bridge laboratory findings with industrial applications.

角蛋白衍生的生物吸附剂由于其可持续和环境友好的特性，已成为水修复应用的高效解决方案。天然丰富的角蛋白具有独特的物理化学性质，对环境无害。它可以从许多生物质来源中提取，如羽毛、蹄、羊毛和头发，其特点是纤维蛋白结构富含功能基团，如二硫、氨基和羧基。这些特性使金属离子和有机污染物同时从污染的水有效结合。该综述重点介绍了基于角蛋白的生物吸附剂的最新进展，该吸附剂吸附金属阳离子和氧阴离子，如Pb2+、Ni2+、Co2+、Zn2+、Cr3+/CrO₄2−、Cd2+、AsO₃3−/AsO₄3−和SeO₃2−/SeO₄2−，以及亚甲基蓝、活性蓝19、结晶紫、甲基橙和酚类化合物等有机污染物，用于可持续的水净化。综述还探讨了提高角蛋白吸附能力、选择性和再生潜力的化学修饰和复合材料。此外，它还指出了重大的研究差距，例如有限的可扩展性以及再生和重用方面的挑战。离子交换、络合、静电相互作用和氢键被认为是角蛋白基材料吸附过程中的关键机制。未来的研究应侧重于开发创新的角蛋白复合材料，采用环保的加工方法，建立机理模型，并进行大规模测试，以将实验室研究结果与工业应用相结合。

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引用次数: 0

Instant catapult steam explosion pretreatment combined with ultrasound-assisted organic acid extraction for efficient calcium recovery from crayfish shells 快速弹射蒸汽爆炸预处理联合超声辅助有机酸萃取小龙虾壳中钙的高效回收

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-01-04 DOI: 10.1016/j.biteb.2025.102522

Hong Wang , Qiang Li , Jianfeng Lu , Mengying Ren , Yuan Song , Fang Tao , Lin Lin , Hongsen Zhang , Bin Zheng , Changhu Xue

To enhance the valorization of crustacean shell waste and address the challenge of low calcium extraction efficiency, this study developed a green and efficient calcium recovery method. In the extraction of calcium from crayfish shells, an instant catapult steam explosion (ICSE) pretreatment was combined with ultrasound-assisted acetic acid extraction. The results showed that ICSE pretreatment significantly reduced the protein content, increasing the relative calcium content from 172.95 mg/g to 201.31 mg/g, while disrupting both the internal and external shell structures. The particle size of the shell powder decreased by 56.03 %, the protein hydrogen-bonding network was disrupted, metastable vaterite formation was promoted, and the onset temperature of thermal degradation was lowered. Based on these findings, the ultrasound-assisted organic acid extraction process was optimized using a Box–Behnken design. Under the optimal conditions, the calcium extraction yield reached 98.84 %, representing an increase of 29.33 % compared to the untreated sample (76.43 %).

为了提高甲壳类废弃物的价值，解决钙提取效率低的问题，本研究开发了一种绿色高效的钙回收方法。在小龙虾壳中提取钙的研究中，将瞬射蒸汽爆炸预处理与超声辅助乙酸提取相结合。结果表明，ICSE预处理显著降低了蛋白质含量，使相对钙含量从172.95 mg/g增加到201.31 mg/g，同时破坏了内壳和外壳结构。壳粉粒径减小56.03%，蛋白质氢键网络被破坏，亚稳水晶石形成加快，热降解起始温度降低。在此基础上，采用Box-Behnken设计对超声辅助有机酸提取工艺进行了优化。在此条件下，钙的提取率达到98.84%，比未处理样品的76.43%提高了29.33%。

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引用次数: 0

Integration of low cost novel membrane in 20 L stack microbial fuel cell technology: Application in powering low-power electronic devices and hydrogen generation in microbial electrolysis cell 低成本新型膜在20l堆式微生物燃料电池技术中的集成：在低功耗电子器件供电和微生物电解电池制氢中的应用

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-01-04 DOI: 10.1016/j.biteb.2026.102543

A. Abubakkar Siddik , Rathinavel Nithya , Alagarsamy Arun

The Novel Cement-Supported Conductive Salts (NCSCS) Proton Exchange Membrane (PEM) is been validated in lab-scale dual-chamber Microbial Fuel Cells (MFCs) using 20 L of septic tank wastewater. The NCSCS PEM is suitable for scalability (20 L × 1 L anode stacks), as proven in the present study under the different Hydraulic Retention Time (HRT) in MFCs operated in the serial and parallel connections. The highest power density (1981 ± 0.96 mW/m³) and Chemical Oxygen Demand removal (84.98 ± 1.02 %) were achieved in the serial configuration of optimized HRT 3 conditions. The current study showcases the MFC-Microbial Electrolysis Cells coupled biohydrogen production rate of 0.02 LH₂/L/d, thereby demonstrating the viability of NCSCS PEM. The cost analysis revealed that the membrane accounted for only 2.28 % of total expenses, with exceptional durability lasting over 352 days without fouling or maintenance. The results demonstrate the potential for large-scale, sustainable bioelectricity generation using NCSCS membrane, making it a practical, affordable solution for wastewater-to-energy applications.

新型水泥支撑导电盐（NCSCS）质子交换膜（PEM）在实验室规模的双室微生物燃料电池（mfc）中使用20 L化粪池废水进行了验证。NCSCS PEM适用于可扩展性（20 L × 1 L阳极堆），在本研究中，在串联和并联连接中运行的mfc中，在不同的水力保留时间（HRT）下证明了这一点。在优化后的hrt3条件串联配置下，功率密度最高（1981±0.96 mW/m3），化学需氧量去除率最高（84.98±1.02%）。本研究展示了mfc -微生物电解细胞耦合生物制氢速率为0.02 LH2/L/d，从而证明了NCSCS PEM的可行性。成本分析显示，该膜仅占总费用的2.28%，具有出色的耐用性，可持续使用352天而不受污染或维护。研究结果表明，利用NCSCS膜进行大规模、可持续的生物发电具有潜力，使其成为废水转化为能源应用的一种实用、经济的解决方案。

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引用次数: 0

Chromium pollution mitigation: Microbial approaches, genetic determinants, and technological innovations 减少铬污染：微生物方法、遗传决定因素和技术创新

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-01-04 DOI: 10.1016/j.biteb.2025.102542

Sagarkumar Joshi , Nidhi Saxena , Devayani R. Tipre , Noopur R. Goyal

Chromium pollution, particularly hexavalent chromium [Cr(VI)], poses a severe global threat to ecosystems and human health due to its toxicity, mobility, and persistence. Although conventional remediation methods can be effective, they are often costly and may generate secondary pollution. Microbial bioremediation provides a sustainable, eco-friendly, and cost-effective alternative that exploits the metabolic capabilities of microorganisms to detoxify Cr(VI) through enzymatic reduction, biosorption, bioaccumulation, and bioprecipitation. This review synthesizes the current knowledge on microbial Cr(VI) detoxification, with an emphasis on chromate reductases, genetic determinants of resistance and reduction, and the influence of environmental factors on bioremediation efficiency. Biofilm-mediated detoxification and the integration of emerging technologies, including nanomaterials, synthetic biology, omics platforms, and artificial intelligence, are highlighted as strategies to enhance the scalability and performance of microbial systems. Despite substantial progress, challenges in field deployment and regulation persist, underscoring the need for integrated genetic, process, and engineering solutions. Emerging tools such as systems biology, engineered biofilms, and hybrid biomaterial platforms show strong potential to overcome limitations of slow kinetics, environmental variability, and regulatory constraints. Closing these gaps will be essential for delivering robust, scalable, and environmentally compatible Cr(VI) remediation solutions.

铬污染，特别是六价铬[Cr(VI)]，由于其毒性、流动性和持久性，对生态系统和人类健康构成严重的全球性威胁。虽然传统的修复方法是有效的，但它们往往是昂贵的，并可能产生二次污染。微生物生物修复提供了一种可持续的、生态友好的、具有成本效益的替代方法，它利用微生物的代谢能力，通过酶还原、生物吸附、生物积累和生物沉淀来解毒Cr（VI）。本文综述了微生物Cr（VI）解毒的最新研究进展，重点介绍了铬酸盐还原酶、抗性和还原的遗传决定因素以及环境因素对生物修复效率的影响。生物膜介导的解毒和新兴技术的整合，包括纳米材料、合成生物学、组学平台和人工智能，被强调为提高微生物系统的可扩展性和性能的策略。尽管取得了实质性进展，但现场部署和监管方面的挑战依然存在，这凸显了对综合遗传、工艺和工程解决方案的需求。系统生物学、工程生物膜和混合生物材料平台等新兴工具显示出克服慢动力学、环境可变性和监管限制的强大潜力。消除这些差距对于提供稳健、可扩展且与环境兼容的Cr（VI）修复解决方案至关重要。

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引用次数: 0

Synergistic enhancement of Wyodak coal bioavailability with sequential chemical treatment and fungal degradation 序贯化学处理和真菌降解协同提高Wyodak煤的生物利用度

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2026-01-04 DOI: 10.1016/j.biteb.2025.102539

Samir Budhathoki , Yanrui Ning , Mohan B. Dangi , Michael A. Urynowicz

This study examined sequential chemical pretreatment and fungal degradation of Wyodak sub-bituminous coal with hydrogen peroxide followed by the fungal degradation with Paecilomyces variotii as the microbial agent. Coal was pretreated across varying concentrations (0.1–6 M) and subsequently assessed for bioavailability in aerobic biometer assays using carbon dioxide (CO₂) production as a quantitative proxy. The highest performance was observed at 3 M hydrogen peroxide, yielding cumulative CO₂ production of ~2.27 mmol/g coal and the maximum initial total organic carbon peaking at 107.37 mg C/g coal, with fungal degradation reducing total organic carbon (TOC) by ~74 mg C/g coal. Three-dimensional excitation-emission matrix fluorescence spectroscopy results showed an increased abundance of aromatic, humic, and fulvic-like organics post‑hydrogen peroxide pretreatment, which declined significantly during fungal treatment, confirming microbial consumption. These results demonstrate synergistic effect of sequential chemical pretreatment and fungal degradation in coal biodegradation, where CO₂ release, and TOC consumption serve as direct indicators of coal bioavailability and microbial activity.

研究了双氧水对Wyodak亚烟煤进行序贯化学预处理和真菌降解，然后以变拟青霉为微生物剂对其进行真菌降解。煤在不同浓度（0.1-6 M）下进行预处理，随后使用二氧化碳（CO2）产量作为定量代理，在好氧生物测量法中评估生物利用度。在过氧化氢浓度为3 M时，CO2累计产量为~2.27 mmol/g煤，初始总有机碳最高峰值为107.37 mg C/g煤，真菌降解使总有机碳（TOC）降低~74 mg C/g煤。三维激发-发射矩阵荧光光谱结果显示，过氧化氢预处理后芳香、腐殖质和黄腐类有机物的丰度增加，真菌处理后丰度显著下降，证实了微生物的消耗。这些结果表明，顺序化学预处理和真菌降解在煤的生物降解过程中具有协同效应，其中CO2释放和TOC消耗是煤的生物利用度和微生物活性的直接指标。

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