Sylvia Natividad-Diaz, Binata Joddar, Wilson Poon, Aibhlin Esparza, Edgar A Borrego, Britanny L Stark
{"title":"Engineered 3D Cardiovascular Tissue Models Within Dynamic Microfluidic Platforms for Personalized Medicine Applications.","authors":"Sylvia Natividad-Diaz, Binata Joddar, Wilson Poon, Aibhlin Esparza, Edgar A Borrego, Britanny L Stark","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"11 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11262465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141749949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/jihp.20190325092056
N. Aravindan, Dinesh Babu Somasundaram, S. Aravindan, Zhongxin Yu, Karthikeyan Subramanian, A. Esmaeili, T. Herman
Background: Our studies identified the loss of Retinal degeneration Protein 3 (RD3) in the predominant infant tumor, neuroblastoma, indicated its novel regulatory function in neuroblastoma pathogenesis and, showed its localization in tissues beyond retina. To explore the possible physiological role of RD3 in regulating the genesis of neuroblastoma, we examined its distribution in human fetal normal tissues. Methods: Constitutive mRNA levels (QPCR, RNAScope), protein expression (immunohistochemistry), and sub-cellular localization of RD3 were investigated in the array (20 sites) of human (n=5) fetal normal tissues. In silico RNA sequencing data from seven independent studies (total n = 407) of human fetal tissues (101 sites) was utilized to validate the differential expression of RD3 in developing tissues. Results: RNAscope and QPCR analysis indicated a steady state of RD3 transcription across the fetal tissues with measurable inter-tissue differences (For e.g. high in GI tissues vs low in adrenal gland). RNA-seq data on varying stages of development clearly indicated stage-dependent dynamic fluctuations in RD3 transcription as the development progress. Notably, RD3 transcription is stably expressed in choroid plexus and are relatively absent in spinal cord through the developmental stages. IHC analysis recognized the presence of RD3 protein in array of fetal normal tissues, the tissue- and cell-specific distinctions in protein expression, and the variances in sub-cellular localization with each tissue. Conclusion: For the first time, the results presented here revealed the tissue-specific transcription, expression and localization of RD3 in fetal tissues. Recognizing the heterogeneous expression of RD3 between and within the fetal tissues signify its possible function beyond photoreceptor cell survival and could shed light on its functional relevance in neuroblastoma genesis and/or evolution.
{"title":"Heterogeneous distribution of Retinal Degeneration Protein 3 in normal human fetal tissues: Exploring the possible relevance to neuroblastoma genesis","authors":"N. Aravindan, Dinesh Babu Somasundaram, S. Aravindan, Zhongxin Yu, Karthikeyan Subramanian, A. Esmaeili, T. Herman","doi":"10.5455/jihp.20190325092056","DOIUrl":"https://doi.org/10.5455/jihp.20190325092056","url":null,"abstract":"Background: Our studies identified the loss of Retinal degeneration Protein 3 (RD3) in the predominant infant tumor, neuroblastoma, indicated its novel regulatory function in neuroblastoma pathogenesis and, showed its localization in tissues beyond retina. To explore the possible physiological role of RD3 in regulating the genesis of neuroblastoma, we examined its distribution in human fetal normal tissues. Methods: Constitutive mRNA levels (QPCR, RNAScope), protein expression (immunohistochemistry), and sub-cellular localization of RD3 were investigated in the array (20 sites) of human (n=5) fetal normal tissues. In silico RNA sequencing data from seven independent studies (total n = 407) of human fetal tissues (101 sites) was utilized to validate the differential expression of RD3 in developing tissues. Results: RNAscope and QPCR analysis indicated a steady state of RD3 transcription across the fetal tissues with measurable inter-tissue differences (For e.g. high in GI tissues vs low in adrenal gland). RNA-seq data on varying stages of development clearly indicated stage-dependent dynamic fluctuations in RD3 transcription as the development progress. Notably, RD3 transcription is stably expressed in choroid plexus and are relatively absent in spinal cord through the developmental stages. IHC analysis recognized the presence of RD3 protein in array of fetal normal tissues, the tissue- and cell-specific distinctions in protein expression, and the variances in sub-cellular localization with each tissue. Conclusion: For the first time, the results presented here revealed the tissue-specific transcription, expression and localization of RD3 in fetal tissues. Recognizing the heterogeneous expression of RD3 between and within the fetal tissues signify its possible function beyond photoreceptor cell survival and could shed light on its functional relevance in neuroblastoma genesis and/or evolution.","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70819816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/JIHP.20181211075659
Hailah M Almohaimeed, Hanan Amin, G. A. El-Aziz, H. A. Saleh
Background: Diabetic peripheral neuropathy (DPN) is a common complication of diabetes mellitus with reported high rates in the Middle East. Objectives: This study aimed to evaluate the role of Arabic gum (AG) in alleviating the histopathological changes, at the ultrastructural level, occur in sciatic nerve in an experimental model of diabetic neuropathy. Materials and Methods: Six groups (n= 10 each) of adult male Sprague-Dawley rats were used in this study; the control, AG-treated, untreated diabetic, diabetic rats treated with metformin alone, diabetic rats treated with metformin and B12 vitamin and diabetic rats treated with metformin, B12 vitamin and AG. Behavioural changes of rats were assessed through open field and hot plat test. Biochemical parameters included blood glucose, insulin levels, lipid profile, oxidants/antioxidants parameters were assessed. Sciatic nerve was dissected, processed and examined histopathologically using the electron microscope. Results: The behavioral changes as well as the lipid profile were significantly improved after all used treatment. The antioxidants indicators included CAT, GSH, SOD, GPx were significantly improved in all treated groups compared to the untreated diabetic group. Sciatic nerve fibers of untreated diabetic rats showed degenerated nerve axons, myelin sheath and Schwann cells. The blood vessels were structurally affected. These ultrastructural changes were markedly improved in all the treated groups specifically that received metformin, vitamin B12 and AG. Conclusion: It could be concluded that Arabic gum combined with metformin and Vitamin B12 had hypoglycemic, hypolipidemic and antioxidant activity which all could be behind its ability to protect sciatic nerve against the diabetes-associated histopathological effects. Based on that a well-designed clinical trial is recommended to confirm its effect of diabetic patients.
{"title":"Arabic gum aracia improves diabetic peripheral neuropathy in rats: Ultrastructural histopathalogical study.","authors":"Hailah M Almohaimeed, Hanan Amin, G. A. El-Aziz, H. A. Saleh","doi":"10.5455/JIHP.20181211075659","DOIUrl":"https://doi.org/10.5455/JIHP.20181211075659","url":null,"abstract":"Background: Diabetic peripheral neuropathy (DPN) is a common complication of diabetes mellitus with reported high rates in the Middle East. Objectives: This study aimed to evaluate the role of Arabic gum (AG) in alleviating the histopathological changes, at the ultrastructural level, occur in sciatic nerve in an experimental model of diabetic neuropathy. Materials and Methods: Six groups (n= 10 each) of adult male Sprague-Dawley rats were used in this study; the control, AG-treated, untreated diabetic, diabetic rats treated with metformin alone, diabetic rats treated with metformin and B12 vitamin and diabetic rats treated with metformin, B12 vitamin and AG. Behavioural changes of rats were assessed through open field and hot plat test. Biochemical parameters included blood glucose, insulin levels, lipid profile, oxidants/antioxidants parameters were assessed. Sciatic nerve was dissected, processed and examined histopathologically using the electron microscope. Results: The behavioral changes as well as the lipid profile were significantly improved after all used treatment. The antioxidants indicators included CAT, GSH, SOD, GPx were significantly improved in all treated groups compared to the untreated diabetic group. Sciatic nerve fibers of untreated diabetic rats showed degenerated nerve axons, myelin sheath and Schwann cells. The blood vessels were structurally affected. These ultrastructural changes were markedly improved in all the treated groups specifically that received metformin, vitamin B12 and AG. Conclusion: It could be concluded that Arabic gum combined with metformin and Vitamin B12 had hypoglycemic, hypolipidemic and antioxidant activity which all could be behind its ability to protect sciatic nerve against the diabetes-associated histopathological effects. Based on that a well-designed clinical trial is recommended to confirm its effect of diabetic patients.","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70819757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/jihp.20190610043258
A. Adefuye, O. J. Adefuye, H. Adeola
Background: Inflammation and inflammatory response have been recognized as an essential hallmark and driver of tumor initiation and progression. Several components of the human seminal fluid (SF) have been previously implicated in initiating an inflammatory response in the healthy cervical epithelium after coitus. However, it was unclear whether the continuous application of SF to dysplastic or neoplastic cervical epithelium would aggravate a pre-existing neoplastic cervical lesion by further regulating the expression of pro-inflammatory cytokines and inflammatory biological pathways. Objectives: This study aimed at investigating gene arrays of inflammatory pathways that can be regulated by SF in neoplastic cervical epithelial cells, as a model to identify pro-inflammatory genes that could exacerbate neoplastic cervical inflammation and promote tumorigenesis in response to SF. Materials and Methods: HeLa-S3 cells were treated with SF (1:50 dilution) or Phosphatebuffered saline (PBS) (control) for 8 hours. Pooled cDNA from SF-treated HeLa-S3 cells were subjected to a quantitative real-time polymerase chain reaction on a TaqMan® 96-well plate human inflammation and cytokine/chemokine array platform, and fold expressions of inflammatory genes were determined. SF-regulated genes were subjected to gene enrichment analysis to evaluate functional pathways involved in cervical cancer progression. Results: SF was found to regulate the expression of arrays of inflammatory genes in neoplastic cervical epithelial cells. SF induced the expression of pro-inflammatory genes such as PTGS1 (2.43↑), IL-6 (4.19↑), IL-8 (38.93↑), Tumor Necrosis Factor (TNF) (26.55↑), IL-1α (68.56↑) and CXCL1 growth-regulated oncogene-alpha (GRO-α) (12.37↑), Toll-like receptor 2 (TLR2) (88.47↑), and transcription factor NF-κB (2.32↑), while downregulating the expression of anti-inflammatory mediators such as IL1R2 (5.0↓), hydroxyprostaglandin dehydrogenase 15-(NAD) (3.12↓), and suppressor of cytokine signaling 5 5 (>100↓). SF-regulated genes clustered into components of eicosanoid signaling, chemokine signaling, cytokine signaling, and TLR2 in HeLa adenocarcinoma cells. Conclusions: The findings of this study suggest that SF can potentially augment cervical tumorigenesis by regulating the induction of arrays of inflammatory pathways in neoplastic cervical epithelial cells. Induction of these inflammatory pathways may play a crucial role in cancer cell angiogenesis, proliferation, invasion, metastasis, and survival.
{"title":"Regulation of pro-inflammatory genes and pathways in neoplastic cervical epithelia pathogenesis","authors":"A. Adefuye, O. J. Adefuye, H. Adeola","doi":"10.5455/jihp.20190610043258","DOIUrl":"https://doi.org/10.5455/jihp.20190610043258","url":null,"abstract":"Background: Inflammation and inflammatory response have been recognized as an essential hallmark and driver of tumor initiation and progression. Several components of the human seminal fluid (SF) have been previously implicated in initiating an inflammatory response in the healthy cervical epithelium after coitus. However, it was unclear whether the continuous application of SF to dysplastic or neoplastic cervical epithelium would aggravate a pre-existing neoplastic cervical lesion by further regulating the expression of pro-inflammatory cytokines and inflammatory biological pathways. Objectives: This study aimed at investigating gene arrays of inflammatory pathways that can be regulated by SF in neoplastic cervical epithelial cells, as a model to identify pro-inflammatory genes that could exacerbate neoplastic cervical inflammation and promote tumorigenesis in response to SF. Materials and Methods: HeLa-S3 cells were treated with SF (1:50 dilution) or Phosphatebuffered saline (PBS) (control) for 8 hours. Pooled cDNA from SF-treated HeLa-S3 cells were subjected to a quantitative real-time polymerase chain reaction on a TaqMan® 96-well plate human inflammation and cytokine/chemokine array platform, and fold expressions of inflammatory genes were determined. SF-regulated genes were subjected to gene enrichment analysis to evaluate functional pathways involved in cervical cancer progression. Results: SF was found to regulate the expression of arrays of inflammatory genes in neoplastic cervical epithelial cells. SF induced the expression of pro-inflammatory genes such as PTGS1 (2.43↑), IL-6 (4.19↑), IL-8 (38.93↑), Tumor Necrosis Factor (TNF) (26.55↑), IL-1α (68.56↑) and CXCL1 growth-regulated oncogene-alpha (GRO-α) (12.37↑), Toll-like receptor 2 (TLR2) (88.47↑), and transcription factor NF-κB (2.32↑), while downregulating the expression of anti-inflammatory mediators such as IL1R2 (5.0↓), hydroxyprostaglandin dehydrogenase 15-(NAD) (3.12↓), and suppressor of cytokine signaling 5 5 (>100↓). SF-regulated genes clustered into components of eicosanoid signaling, chemokine signaling, cytokine signaling, and TLR2 in HeLa adenocarcinoma cells. Conclusions: The findings of this study suggest that SF can potentially augment cervical tumorigenesis by regulating the induction of arrays of inflammatory pathways in neoplastic cervical epithelial cells. Induction of these inflammatory pathways may play a crucial role in cancer cell angiogenesis, proliferation, invasion, metastasis, and survival.","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70819825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/jihp.20180726092233
M. Aboud, M. M. Kadhim
Giant cell fibroblastoma (GCF) is an unusual tumor of childhood, primarily occurring in the superficial soft tissues. It is often presented in early childhood as a slowly growing, infiltrative subcutaneous mass at a wide variety of sites. Aim: to present such an interesting rare case of pediatric chest wall lesion. Case report: A 4 years old male patient was admitted to the pediatric surgery unit with complaints of a slow-growing painless palpable, firm swelling in the left chest wall below the left breast. All initial assessments were done, surgery was created. Histopathology and immunohistochemistry revealed the distinctive images of GCF. Conclusion: Although the giant cell fibroblastoma is a rare chest wall tumor in the pediatric age group, it must be considered in differential diagnosis. The histopathology approached has more precise and distinctive images for decision-making regarding the surgery.
{"title":"Giant cell fibroblastoma a rare chest wall tumor in a 4 years old boy: a case report","authors":"M. Aboud, M. M. Kadhim","doi":"10.5455/jihp.20180726092233","DOIUrl":"https://doi.org/10.5455/jihp.20180726092233","url":null,"abstract":"Giant cell fibroblastoma (GCF) is an unusual tumor of childhood, primarily occurring in the superficial soft tissues. It is often presented in early childhood as a slowly growing, infiltrative subcutaneous mass at a wide variety of sites. Aim: to present such an interesting rare case of pediatric chest wall lesion. Case report: A 4 years old male patient was admitted to the pediatric surgery unit with complaints of a slow-growing painless palpable, firm swelling in the left chest wall below the left breast. All initial assessments were done, surgery was created. Histopathology and immunohistochemistry revealed the distinctive images of GCF. Conclusion: Although the giant cell fibroblastoma is a rare chest wall tumor in the pediatric age group, it must be considered in differential diagnosis. The histopathology approached has more precise and distinctive images for decision-making regarding the surgery.","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70819654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/jihp.20190824114245
Jude Ogechukwu Okoye, Success Iwuanyanwu, Goodnews Ubong Nathaniel, O. Agboola, C. Offor, John Kennedy Belonwu
Background: Aflatoxin B1 (AFB1), hepatitis B virus (HBV) and p53 mutation are major risk factors for liver cancer. However, the presence of AFB1 and expression of mutant p53 protein during active HBV replication (nuclear expression of Hepatitis B Core Antigen; HBcAg) and HBV clearance (nuclear expression of HBcAg) is still under-investigated in Africa. Objectives: This study assessed the prevalence, extent of expression, interaction, and risk associated with single and binary expression of AFB1, HBcAg and mutant p53 (mtp53) in liver diseases in relation to age and sex. Materials and Methods: This retrospective study included 14 and 74 cases of liver cancer (LC) and chronic hepatitis, respectively. Tissues were histochemically and immunohistochemically stained, scored and documented as positive (+) or negative (-) for antigen. Result: In this study, the prevalence of AFB1, HBcAg, and mtp53 were 76.1%, 40.9%, and 61.4%, respectively. Higher prevalence and expression of AFB1 was observed in chronic hepatitis than in LC (p= 0.035 and 0.028, respectively). Higher prevalence of AFB1 and mtp53 and lower prevalence of HBcAg were observed in females than in males (p= 0.186, 0.0003 and 0.062, respectively). Cytoplasmic expression of HBcAg was higher in males (58 than in females were 58.6% (17/29) and 14.3% (1/7), respectively (p= 0.088). AFB1+ and HBcAg+ females were more and less likely to develop LC than males (OR: 3.00 and 0.39, 95%CI: 0.10-4.18 and 0.44-12.20, p= 0.012 and 0.003), respectively. AFB1+mtp53- (23.9%), AFB1+mtp53+ (52.3%), HBcAg+mtp53- (13.6%), HBcAg+mtp53+ (27.3%) and AFB1+HBcAg+ (34.1%) increased liver cancer risk (OR: 6.45, 21.50, 2.80, 0.78, and 5.11; 95%CI: 0.89-29.29, 0.95-43.86, 2.96-156.13, 0.60-13.01, and 0.14-4.25; p= 0.057, 0.002, 0.189, 0.772, and 0.067, respectively). Conclusion: This study suggests that women are at a higher risk of AFB1 exposure and p53 mutation, but are at a lower risk of viral replication than men.
{"title":"Mutant p53 protein is prevalent in Aflatoxin B1 than in Hepatitis B Core antigen associated chronic liver diseases in Southern Nigeria","authors":"Jude Ogechukwu Okoye, Success Iwuanyanwu, Goodnews Ubong Nathaniel, O. Agboola, C. Offor, John Kennedy Belonwu","doi":"10.5455/jihp.20190824114245","DOIUrl":"https://doi.org/10.5455/jihp.20190824114245","url":null,"abstract":"Background: Aflatoxin B1 (AFB1), hepatitis B virus (HBV) and p53 mutation are major risk factors for liver cancer. However, the presence of AFB1 and expression of mutant p53 protein during active HBV replication (nuclear expression of Hepatitis B Core Antigen; HBcAg) and HBV clearance (nuclear expression of HBcAg) is still under-investigated in Africa. Objectives: This study assessed the prevalence, extent of expression, interaction, and risk associated with single and binary expression of AFB1, HBcAg and mutant p53 (mtp53) in liver diseases in relation to age and sex. Materials and Methods: This retrospective study included 14 and 74 cases of liver cancer (LC) and chronic hepatitis, respectively. Tissues were histochemically and immunohistochemically stained, scored and documented as positive (+) or negative (-) for antigen. Result: In this study, the prevalence of AFB1, HBcAg, and mtp53 were 76.1%, 40.9%, and 61.4%, respectively. Higher prevalence and expression of AFB1 was observed in chronic hepatitis than in LC (p= 0.035 and 0.028, respectively). Higher prevalence of AFB1 and mtp53 and lower prevalence of HBcAg were observed in females than in males (p= 0.186, 0.0003 and 0.062, respectively). Cytoplasmic expression of HBcAg was higher in males (58 than in females were 58.6% (17/29) and 14.3% (1/7), respectively (p= 0.088). AFB1+ and HBcAg+ females were more and less likely to develop LC than males (OR: 3.00 and 0.39, 95%CI: 0.10-4.18 and 0.44-12.20, p= 0.012 and 0.003), respectively. AFB1+mtp53- (23.9%), AFB1+mtp53+ (52.3%), HBcAg+mtp53- (13.6%), HBcAg+mtp53+ (27.3%) and AFB1+HBcAg+ (34.1%) increased liver cancer risk (OR: 6.45, 21.50, 2.80, 0.78, and 5.11; 95%CI: 0.89-29.29, 0.95-43.86, 2.96-156.13, 0.60-13.01, and 0.14-4.25; p= 0.057, 0.002, 0.189, 0.772, and 0.067, respectively). Conclusion: This study suggests that women are at a higher risk of AFB1 exposure and p53 mutation, but are at a lower risk of viral replication than men.","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70819832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.5455/JIHP.20181014075121
S. Dawa, O. Bassyoni
Background: Background: Lymph node metastasis (LNM) is a main route of endometrial carcinoma (EC) spread and it plays a chief prognostic role. Our objective is to find LMN predictors through investigating lymph vessel invasion (LVI), and lymphatic vessel density (LVD), in addition to VEGF-C expression in the primary tumor site of the EC. Material and Methods: A retrospective Immunohistochemical study applying VEGF-C and D2-40 antibodies on 40 EC cases, in addition to 20 cases of proliferative endometrium, and 20 cases with atypical endometrial hyperplasia as control groups. The studied cases were screened for expression of VEGF-C, D2-40- LVI, and LVD. Statistical analysis with the correlation of the findings to various clinicopathological parameters were achieved. Results: Expression of VEGF-C was moderate to high in 87.5% of EC cases, only mild to moderate focal staining in atypical hyperplasia cases, and exclusively negative in proliferative endometrium cases. VEGF-C expression could independently predict LNM with sensitivity, specificity , PPV, NPV, and accuracy of (100, 51.6, 62.5, 100, 62.5%)respectively. D2-40-LVI detection system is more sensitive in predicting LNM in EC than routine H&E stained section. It was detected in 9/9of EC with LNM cases ,(sensetivity100%) compared to 7/9 cases detected by H&E staining (sensetivity77.8%). D2-40 -LVI detection system could predict LNM with sensitivity, specificity, PPV, NPV, and accuracy of (100, 67.7, 47.4, 100, 75%. )respectively. D2-40-LVI ,Peritumoral LVD, and VEGF-C expression showed a significant correlation to LNM (p
{"title":"The impact of examining VEGF-C expression, D2-40 based detection of LVI, and LVD on the Prediction of Lymph Node Metastasis in Endometrial Carcinoma.","authors":"S. Dawa, O. Bassyoni","doi":"10.5455/JIHP.20181014075121","DOIUrl":"https://doi.org/10.5455/JIHP.20181014075121","url":null,"abstract":"Background: Background: Lymph node metastasis (LNM) is a main route of endometrial carcinoma (EC) spread and it plays a chief prognostic role. Our objective is to find LMN predictors through investigating lymph vessel invasion (LVI), and lymphatic vessel density (LVD), in addition to VEGF-C expression in the primary tumor site of the EC. Material and Methods: A retrospective Immunohistochemical study applying VEGF-C and D2-40 antibodies on 40 EC cases, in addition to 20 cases of proliferative endometrium, and 20 cases with atypical endometrial hyperplasia as control groups. The studied cases were screened for expression of VEGF-C, D2-40- LVI, and LVD. Statistical analysis with the correlation of the findings to various clinicopathological parameters were achieved. Results: Expression of VEGF-C was moderate to high in 87.5% of EC cases, only mild to moderate focal staining in atypical hyperplasia cases, and exclusively negative in proliferative endometrium cases. VEGF-C expression could independently predict LNM with sensitivity, specificity , PPV, NPV, and accuracy of (100, 51.6, 62.5, 100, 62.5%)respectively. D2-40-LVI detection system is more sensitive in predicting LNM in EC than routine H&E stained section. It was detected in 9/9of EC with LNM cases ,(sensetivity100%) compared to 7/9 cases detected by H&E staining (sensetivity77.8%). D2-40 -LVI detection system could predict LNM with sensitivity, specificity, PPV, NPV, and accuracy of (100, 67.7, 47.4, 100, 75%. )respectively. D2-40-LVI ,Peritumoral LVD, and VEGF-C expression showed a significant correlation to LNM (p","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70819857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.5455/jihp.20180130052413
P. Shetty, U. Shetty, Chethana Dinakar
Specialization has become a fundamental characteristic of contemporary medical practice. The designation “Oral and Maxillofacial Pathology†has traditionally been referred to all pathologies of Oral and Maxillofacial region. It has also been an important bridging specialty between dentistry and medicine. Although we continue to work within the same set of constraints provided by the product of our current educational and training programs, it is time to reconsider this product for sustained growth in the specialty of oral and maxillofacial pathology. We must give thought to innovation within advanced education programs, which would build on the traditional strengths of surgical oral and maxillofacial pathology, and to a greater emphasis on science and academic accomplishment in the form of laboratory-based research. The potential overall contributions of the versatile oral and maxillofacial pathologist to the health care delivery system must be emphasized. Contributions could include active participation in direct patient care for those with oral disease within both the hospital environment and dental school setting. Management of oral and maxillofacial diseases and conditions by the oral and maxillofacial pathologist must also assume a greater level of importance, not just in dentistry but within the health care delivery system as well. The preservation and growth of oral and maxillofacial pathology requires greater emphasis on oral pathologist’s role and value within the educational community, the health care delivery system, and the research community. This paper highlights the new area and possible future scope for oral and maxillofacial pathology in dental healthcare system in India
{"title":"ORAL & MAXILLOFACIAL PATHOLOGY IN DENTAL EDUCATION: A PERSPECTIVE","authors":"P. Shetty, U. Shetty, Chethana Dinakar","doi":"10.5455/jihp.20180130052413","DOIUrl":"https://doi.org/10.5455/jihp.20180130052413","url":null,"abstract":"Specialization has become a fundamental characteristic of contemporary medical practice. The designation “Oral and Maxillofacial Pathology†has traditionally been referred to all pathologies of Oral and Maxillofacial region. It has also been an important bridging specialty between dentistry and medicine. Although we continue to work within the same set of constraints provided by the product of our current educational and training programs, it is time to reconsider this product for sustained growth in the specialty of oral and maxillofacial pathology. We must give thought to innovation within advanced education programs, which would build on the traditional strengths of surgical oral and maxillofacial pathology, and to a greater emphasis on science and academic accomplishment in the form of laboratory-based research. The potential overall contributions of the versatile oral and maxillofacial pathologist to the health care delivery system must be emphasized. Contributions could include active participation in direct patient care for those with oral disease within both the hospital environment and dental school setting. Management of oral and maxillofacial diseases and conditions by the oral and maxillofacial pathologist must also assume a greater level of importance, not just in dentistry but within the health care delivery system as well. The preservation and growth of oral and maxillofacial pathology requires greater emphasis on oral pathologist’s role and value within the educational community, the health care delivery system, and the research community. This paper highlights the new area and possible future scope for oral and maxillofacial pathology in dental healthcare system in India","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"6 1","pages":"11-14"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70819500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.5455/JIHP.20180726085949
LizbethRaju LizbethRaju, ShwethaNambiar ShwethaNambiar, D. Augustine, SowmyaS, Vanishri C. Haragannavar, AshokBabu AshokBabu, Roopa S. Rao
Background: The extensive evolution of Histopathology can be mainly attributed to the availability of a wide array of stains. Staining has made possible the identification of various tissue structures under microscope aiding in appropriate diagnosis. However, the present era of increasing importance to ecology has necessitated the requisite for natural dyes. Unlike synthetic dyes, natural dyes are less toxic, biodegradable and are eco-friendly. Haematoxylin and Eosin are the routinely used stains in histopathology wherein eosin is a synthetic stain. Various attempts have been made to substitute eosin with a natural dye, one among which is Lawsonia inermis (Henna). Nevertheless, the staining efficacy of henna extract on FFPE (formalin-fixed paraffin embedded) oral tissues, as a counterstain to haematoxylins is yet to be determined. Aim: The study aims to analyse the use of Lawsonia inermis extract as a possible substitute for eosin stain in paraffin embedded oral tissues. Methodology: The colouring component of dried leaves of Henna was extracted using Maceration and Soxhlet method. 4µ sections of 20 paraffin embedded oral tissues of normal oral mucosal tissues and OSCC tissues each, were stained using the extract and counterstained to haematoxylin and studied for its staining efficacy. Statistical Analysis: Chi square test was done and noted for any significant results. Results: On comparing the staining efficacy of henna while using different extraction methods, Soxhlet method was better than maceration method by 20%, however statistically the results were insignificant. Staining efficacy of Henna with and without the mordant were statistically significant wherein staining with mordant gave inferior quality stain. Henna stain, when compared to eosin, comparable results were found, with eosin being slightly better by 15%. Conclusion: In this era of increasing importance to ecology, henna may well prove to be an effective alternative to eosin in histological sections of normal and pathological tissues as both the stains gave comparable results.
{"title":"Lawsonia inermis (Henna) extract: A possible natural substitute to Eosin stain","authors":"LizbethRaju LizbethRaju, ShwethaNambiar ShwethaNambiar, D. Augustine, SowmyaS, Vanishri C. Haragannavar, AshokBabu AshokBabu, Roopa S. Rao","doi":"10.5455/JIHP.20180726085949","DOIUrl":"https://doi.org/10.5455/JIHP.20180726085949","url":null,"abstract":"Background: The extensive evolution of Histopathology can be mainly attributed to the availability of a wide array of stains. Staining has made possible the identification of various tissue structures under microscope aiding in appropriate diagnosis. However, the present era of increasing importance to ecology has necessitated the requisite for natural dyes. Unlike synthetic dyes, natural dyes are less toxic, biodegradable and are eco-friendly. Haematoxylin and Eosin are the routinely used stains in histopathology wherein eosin is a synthetic stain. Various attempts have been made to substitute eosin with a natural dye, one among which is Lawsonia inermis (Henna). Nevertheless, the staining efficacy of henna extract on FFPE (formalin-fixed paraffin embedded) oral tissues, as a counterstain to haematoxylins is yet to be determined. Aim: The study aims to analyse the use of Lawsonia inermis extract as a possible substitute for eosin stain in paraffin embedded oral tissues. Methodology: The colouring component of dried leaves of Henna was extracted using Maceration and Soxhlet method. 4µ sections of 20 paraffin embedded oral tissues of normal oral mucosal tissues and OSCC tissues each, were stained using the extract and counterstained to haematoxylin and studied for its staining efficacy. Statistical Analysis: Chi square test was done and noted for any significant results. Results: On comparing the staining efficacy of henna while using different extraction methods, Soxhlet method was better than maceration method by 20%, however statistically the results were insignificant. Staining efficacy of Henna with and without the mordant were statistically significant wherein staining with mordant gave inferior quality stain. Henna stain, when compared to eosin, comparable results were found, with eosin being slightly better by 15%. Conclusion: In this era of increasing importance to ecology, henna may well prove to be an effective alternative to eosin in histological sections of normal and pathological tissues as both the stains gave comparable results.","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"6 1","pages":"54-60"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70819614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：