Pub Date : 2017-01-01DOI: 10.5455/JIHP.20170228083758
Ahlem Bdioui, N. Abdessayed, Nozha Mhamdi, Marwa Guerfela, A. B. Abdelkader, M. Mokni
Blastomycosis is a nonopportunistic fungal infection caused by Blastomyces dermatitidis. Infection of the central nervous system is an uncommon localization of this disease occurring only in 5-10% of cases. We report a new case of a 57-year-old woman, who was admitted in the neurosurgical department for severe headaches, vomiting, and a visual disturbance. Physical and biological investigations were normal. Magnetic resonance imaging leaded to find an intracranial and extracranial expansive process, measuring 42 mm in length. The microscopic examination revealed a chronic granulomatous inflammation, associating epitheloid and multinucleated giant cells containing thick walled budding yeast-like forms. Grocott-Gomori methenamine-silver (GMS) staining leaded to confirm its fungal nature and it was precisely, B. dermatitidis. The post-operative course was complicated by cerebral edema associated with an important cerebral hemorrhage. The patient died 11 days after the surgery. Cerebral involvement remains a serious but uncommon manifestation of Blastomycosis. It should be suspected in front of a granulomatous inflammation with the presence of spore, on the pathological examination. Delay in diagnosis has been reported to play a significant role in the high mortality rate.
{"title":"Cerebral Blastomycosis in a Tunisian Woman","authors":"Ahlem Bdioui, N. Abdessayed, Nozha Mhamdi, Marwa Guerfela, A. B. Abdelkader, M. Mokni","doi":"10.5455/JIHP.20170228083758","DOIUrl":"https://doi.org/10.5455/JIHP.20170228083758","url":null,"abstract":"Blastomycosis is a nonopportunistic fungal infection caused by Blastomyces dermatitidis. Infection of the central nervous system is an uncommon localization of this disease occurring only in 5-10% of cases. We report a new case of a 57-year-old woman, who was admitted in the neurosurgical department for severe headaches, vomiting, and a visual disturbance. Physical and biological investigations were normal. Magnetic resonance imaging leaded to find an intracranial and extracranial expansive process, measuring 42 mm in length. The microscopic examination revealed a chronic granulomatous inflammation, associating epitheloid and multinucleated giant cells containing thick walled budding yeast-like forms. Grocott-Gomori methenamine-silver (GMS) staining leaded to confirm its fungal nature and it was precisely, B. dermatitidis. The post-operative course was complicated by cerebral edema associated with an important cerebral hemorrhage. The patient died 11 days after the surgery. Cerebral involvement remains a serious but uncommon manifestation of Blastomycosis. It should be suspected in front of a granulomatous inflammation with the presence of spore, on the pathological examination. Delay in diagnosis has been reported to play a significant role in the high mortality rate.","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"1 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70818982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.5455/jihp.20170201043340
S. Elbasateeny, Taiseer R. Ibrahim, I. Harera
Objective: This study aimed to correlate the immunohistochemical expression of fatty acid synthase (FASN) and HER-2 with the clinicopathological variables in breast infiltrating duct carcinoma (IDC) to identify the impact of marker expression on tumor behavior and mammary carcinogenesis and to detect the correlations between the two markers. Methods: Immunohistochemical expressions of FASN and HER-2 were evaluated in 77 breast case including 10 normal breast, 12 ductal carcinoma in situ (DCIS) and 55 IDC and correlated with clinicopathological variables. The obtained data were statistically analyzed. Results: FASN was overexpressed in 91.7% and 70.9% of DCIS and IDC, respectively, with a significant difference from the normal (P = 0.000). In IDC, the expression of FASN was significantly more expressed in low-grade IDC (P = 0.031). FASN was significantly upregulated in larger tumors and lymph node metastasis (P = 0.017 and P = 0.046, respectively). The expression of FASN had a significant negative correlation with progesterone receptor (PR) (P = 0.05). HER-2 was overexpressed in 50% and 25.45% of DCIS and IDC, respectively, with a significant difference from the normal (P = 0.026). HER-2 was only expressed in high-grade DCIS (P = 0.007). In IDC, the expression of HER-2 was significantly up-regulated in larger tumors and lymph node metastasis (P = 0.033 and P = 0.015, respectively). The expression of HER-2 had a significant negative correlation with estrogen receptor (ER) and PR (P = 0.001 and P = 0.023, respectively). Correlation between the expression of FASN and HER-2 in IDC, revealed a significant positive correlation (Spearman correlation [r] = 0.374, P = 0.005). Conclusions: We concluded that overexpression of FASN and HER-2 may facilitate the early diagnosis of IDC. In IDC, their over expression might warn of a more aggressive course. Our correlation found that FAS expression is closely associated with over expression of HER-2 in IDC.
{"title":"Clinicopathological Significance and Correlation of Fatty Acid Synthase (FASN) and HER2 Expression in Infiltrating Duct Carcinoma of the Breast","authors":"S. Elbasateeny, Taiseer R. Ibrahim, I. Harera","doi":"10.5455/jihp.20170201043340","DOIUrl":"https://doi.org/10.5455/jihp.20170201043340","url":null,"abstract":"Objective: This study aimed to correlate the immunohistochemical expression of fatty acid synthase (FASN) and HER-2 with the clinicopathological variables in breast infiltrating duct carcinoma (IDC) to identify the impact of marker expression on tumor behavior and mammary carcinogenesis and to detect the correlations between the two markers. Methods: Immunohistochemical expressions of FASN and HER-2 were evaluated in 77 breast case including 10 normal breast, 12 ductal carcinoma in situ (DCIS) and 55 IDC and correlated with clinicopathological variables. The obtained data were statistically analyzed. Results: FASN was overexpressed in 91.7% and 70.9% of DCIS and IDC, respectively, with a significant difference from the normal (P = 0.000). In IDC, the expression of FASN was significantly more expressed in low-grade IDC (P = 0.031). FASN was significantly upregulated in larger tumors and lymph node metastasis (P = 0.017 and P = 0.046, respectively). The expression of FASN had a significant negative correlation with progesterone receptor (PR) (P = 0.05). HER-2 was overexpressed in 50% and 25.45% of DCIS and IDC, respectively, with a significant difference from the normal (P = 0.026). HER-2 was only expressed in high-grade DCIS (P = 0.007). In IDC, the expression of HER-2 was significantly up-regulated in larger tumors and lymph node metastasis (P = 0.033 and P = 0.015, respectively). The expression of HER-2 had a significant negative correlation with estrogen receptor (ER) and PR (P = 0.001 and P = 0.023, respectively). Correlation between the expression of FASN and HER-2 in IDC, revealed a significant positive correlation (Spearman correlation [r] = 0.374, P = 0.005). Conclusions: We concluded that overexpression of FASN and HER-2 may facilitate the early diagnosis of IDC. In IDC, their over expression might warn of a more aggressive course. Our correlation found that FAS expression is closely associated with over expression of HER-2 in IDC.","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"5 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70819329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.5455/jihp.20170313075507
Anuradha Rao, S. Gopal, Ranjini Kudva, R. Shenoy, Sandeep Kumar
Ganglioneuroma is a benign neurogenic tumor composed of ganglion cells, nerve fibers, and supporting cells. Intestinal ganglioneuromas are rare tumors more often identified in the adults as solitary polypoid ganglioneuromas, ganglioneuromatous polyposis and diffuse ganglioneuromatosis, presenting with abdominal pain, diarrhea, thickening of the bowel wall, and stricture formation. Diffuse ganglioneuromatosis is typically associated with multiple endocrine neoplasia, neurofibromatosis Type 1 and Cowden syndrome. In this case report, we describe a case of diffuse and polypoid ganglioneuromatosis along with peri-intestinal neurofibroma in a 30-year-old female with no syndromic afflictions. A 30-year-old female was investigated for recurrent alternating episodes of constipation and diarrhea since 15 days, associated with abdominal distension and palpable mass per abdomen. On contrast enhanced computed tomography abdomen and pelvis, multifocal strictures with subacute intestinal obstruction was noted with possibilities of Crohn’s disease or tuberculosis. Surgically resected terminal ileum and ascending colon had multiple linear ulcers, pseudopolyps, and cobblestone appearance. On histopathological examination of the resected specimen, diagnosis of intestinal ganglioneuromatosis (polypoid and diffuse) with suture granuloma and peri-intestinal neurofibroma was given. History elicited for similar prior complaints showed nonspecific ileocolitis on biopsy 2 years earlier. She also gave history of surgery for resection and anastomosis 6 years back. We report a patient with intestinal ganglioneuromatosis in whom clinical and radiological findings mimicked those of Crohn’s disease/tuberculosis. Despite its rarity, intestinal ganglioneuromatosis should be considered in patients with features suggestive of Crohn’s disease/tuberculosis on radiological investigations who have nonspecific mucosal biopsy findings and intractable symptoms despite medical treatment. Ganglioneuroma is a benign neurogenic tumor composed of ganglion cells, nerve fibers and supporting cells. In this case report, we describe a case of 30-year-old female with diffuse and polypoid ganglioneuromatosis along with peri-intestinal neurofibroma with no syndromic afflictions in whom clinical and radiological findings mimicked those of Crohn’s disease/tuberculosis. Despite its rarity, intestinal ganglioneuromatosis should be considered in patients with features suggestive of Crohn’s disease/tuberculosis.
{"title":"Intestinal Ganglioneuromatosis with Peri-intestinal Neurofibroma Limited to the Gastrointestinal Tract Clinically and Morphologically Mimicking Crohn's Disease -","authors":"Anuradha Rao, S. Gopal, Ranjini Kudva, R. Shenoy, Sandeep Kumar","doi":"10.5455/jihp.20170313075507","DOIUrl":"https://doi.org/10.5455/jihp.20170313075507","url":null,"abstract":"Ganglioneuroma is a benign neurogenic tumor composed of ganglion cells, nerve fibers, and supporting cells. Intestinal ganglioneuromas are rare tumors more often identified in the adults as solitary polypoid ganglioneuromas, ganglioneuromatous polyposis and diffuse ganglioneuromatosis, presenting with abdominal pain, diarrhea, thickening of the bowel wall, and stricture formation. Diffuse ganglioneuromatosis is typically associated with multiple endocrine neoplasia, neurofibromatosis Type 1 and Cowden syndrome. In this case report, we describe a case of diffuse and polypoid ganglioneuromatosis along with peri-intestinal neurofibroma in a 30-year-old female with no syndromic afflictions. A 30-year-old female was investigated for recurrent alternating episodes of constipation and diarrhea since 15 days, associated with abdominal distension and palpable mass per abdomen. On contrast enhanced computed tomography abdomen and pelvis, multifocal strictures with subacute intestinal obstruction was noted with possibilities of Crohn’s disease or tuberculosis. Surgically resected terminal ileum and ascending colon had multiple linear ulcers, pseudopolyps, and cobblestone appearance. On histopathological examination of the resected specimen, diagnosis of intestinal ganglioneuromatosis (polypoid and diffuse) with suture granuloma and peri-intestinal neurofibroma was given. History elicited for similar prior complaints showed nonspecific ileocolitis on biopsy 2 years earlier. She also gave history of surgery for resection and anastomosis 6 years back. We report a patient with intestinal ganglioneuromatosis in whom clinical and radiological findings mimicked those of Crohn’s disease/tuberculosis. Despite its rarity, intestinal ganglioneuromatosis should be considered in patients with features suggestive of Crohn’s disease/tuberculosis on radiological investigations who have nonspecific mucosal biopsy findings and intractable symptoms despite medical treatment. Ganglioneuroma is a benign neurogenic tumor composed of ganglion cells, nerve fibers and supporting cells. In this case report, we describe a case of 30-year-old female with diffuse and polypoid ganglioneuromatosis along with peri-intestinal neurofibroma with no syndromic afflictions in whom clinical and radiological findings mimicked those of Crohn’s disease/tuberculosis. Despite its rarity, intestinal ganglioneuromatosis should be considered in patients with features suggestive of Crohn’s disease/tuberculosis.","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"5 1","pages":"99-102"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70818995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.5455/jihp.20161112104025
L. Nigam, K. Kanodia, A. Vanikar, Rashmi D. Patel, K. Suthar, S. J. Rizvi
Primary adrenal lymphoma is a rare entity and may be suspected in patients having bilateral adrenal masses, with/without lymphadenopathy, and with/without adrenal insufficiency. We report a rare case of a 45-year-old man who presented with pain in the abdomen, with no signs of adrenal insufficiency and bilateral adrenal masses on imaging. Light microscopy findings with immunohistochemistry and flow cytometry confirmed the diagnosis of diffuse large B-cell lymphoma. The patient was offered cyclophosphamide, adriamycin, vincristine, and prednisolone chemotherapy regimen and doing well till the last follow-up.
{"title":"Primary Adrenal Lymphoma Infiltrating in to Pancreas: A Rare Cause of Adrenomegaly -","authors":"L. Nigam, K. Kanodia, A. Vanikar, Rashmi D. Patel, K. Suthar, S. J. Rizvi","doi":"10.5455/jihp.20161112104025","DOIUrl":"https://doi.org/10.5455/jihp.20161112104025","url":null,"abstract":"Primary adrenal lymphoma is a rare entity and may be suspected in patients having bilateral adrenal masses, with/without lymphadenopathy, and with/without adrenal insufficiency. We report a rare case of a 45-year-old man who presented with pain in the abdomen, with no signs of adrenal insufficiency and bilateral adrenal masses on imaging. Light microscopy findings with immunohistochemistry and flow cytometry confirmed the diagnosis of diffuse large B-cell lymphoma. The patient was offered cyclophosphamide, adriamycin, vincristine, and prednisolone chemotherapy regimen and doing well till the last follow-up.","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"5 1","pages":"25-28"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70819113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.5455/jihp.20160622013430
Sowmya J. Rao, J. Rao, J. Bm, Varsha Vk
Myofibroblasts are one of the most controversial cells in recent times. Ever since its first discovery, numerous discussions have been done on its illusive nature and functions. They are commonly considered as smooth muscle like fibroblasts. Their presence and distribution in normal and pathological conditions are still not clear since they are difficult to identify with the routine histological techniques. Recent studies have shown their ubiquitous presence in the body tissues hence suggesting their important role in both physiological functioning and pathological conditions. This review discusses briefly the cell in terms of its definition, possible precursors; mechanism involved in its modulation, most importantly how to differentiate it from its nearest counterparts such as fibroblasts and smooth muscle cells and finally its fundamental role in physiology and pathology.
{"title":"Mysterious Myofibroblast: A Cell with Diverse Origin and Multiple Functions","authors":"Sowmya J. Rao, J. Rao, J. Bm, Varsha Vk","doi":"10.5455/jihp.20160622013430","DOIUrl":"https://doi.org/10.5455/jihp.20160622013430","url":null,"abstract":"Myofibroblasts are one of the most controversial cells in recent times. Ever since its first discovery, numerous discussions have been done on its illusive nature and functions. They are commonly considered as smooth muscle like fibroblasts. Their presence and distribution in normal and pathological conditions are still not clear since they are difficult to identify with the routine histological techniques. Recent studies have shown their ubiquitous presence in the body tissues hence suggesting their important role in both physiological functioning and pathological conditions. This review discusses briefly the cell in terms of its definition, possible precursors; mechanism involved in its modulation, most importantly how to differentiate it from its nearest counterparts such as fibroblasts and smooth muscle cells and finally its fundamental role in physiology and pathology.","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"5 1","pages":"12-17"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70819210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.5455/jihp.20170201121719
A. A. Bary, A. Bary
Objective: The pathogenesis of psoriasis involves T-cell mediated immunologic response, keratinocytes hyperproliferation, and resistance to apoptosis. Methotrexate is one of the most reliable modalities in the treatment of psoriasis that target those changes. The study of those variables before and after methotrexate therapy may answer the question: Which of those variables contributes more to the clinical severity of the disease and is more valuable to be targeted in psoriasis treatment. Patients and Methods: A total of 50 cases of psoriasis vulgaris were included, 25 patients received methotrexate therapy. Skin biopsies of psoriatic skin lesions before and after treatment were examined histologically for measurement of epidermal thickness and grading of dermal inflammation. Immunostaining for Ki-67 and p53 was done for assessment of keratinocyte proliferation and apoptosis. Results were correlated with clinical severity of the disease, assessed by psoriatic area and severity index (PASI) score. 15 biopsies of normal skin were included as control. Results: Dermal inflammation, Ki-67%, and p53% were significantly higher in psoriatic skin lesion than in normal skin. Those changes were significantly correlated with PASI score. Multiple logistic regression models revealed that Ki-67 was the most significant variable contributing to clinical severity. One unit change in ki-67% can explain 1.2 unit changes in PASI score with 97% sensitivity, 40% specificity and 25% cut-off value. PASI score, dermal inflammation, Ki-67% and P53% were significantly reduced after methotrexate therapy. No significant difference was detected between the percent reduction in PASI score (81%) and that of Ki-67% (70%). Conclusion: Keratinocyte proliferation was the most significant variable contributing to the clinical severity of psoriasis and it was the single parameter that showed parallel changes to PASI score after methotrexate therapy. Keratinocyte proliferation may be considered as the stimulus that induces all other pathological and clinical changes in psoriasis and should be targeted by therapy.
{"title":"Study of Keratinocyte Proliferation, Apoptosis and Dermal Inflammation in Psoriatic Skin Lesions Before and After Methotrexate Therapy: Which Change Is Contributing More to Clinical Severity of the Disease?","authors":"A. A. Bary, A. Bary","doi":"10.5455/jihp.20170201121719","DOIUrl":"https://doi.org/10.5455/jihp.20170201121719","url":null,"abstract":"Objective: The pathogenesis of psoriasis involves T-cell mediated immunologic response, keratinocytes hyperproliferation, and resistance to apoptosis. Methotrexate is one of the most reliable modalities in the treatment of psoriasis that target those changes. The study of those variables before and after methotrexate therapy may answer the question: Which of those variables contributes more to the clinical severity of the disease and is more valuable to be targeted in psoriasis treatment. Patients and Methods: A total of 50 cases of psoriasis vulgaris were included, 25 patients received methotrexate therapy. Skin biopsies of psoriatic skin lesions before and after treatment were examined histologically for measurement of epidermal thickness and grading of dermal inflammation. Immunostaining for Ki-67 and p53 was done for assessment of keratinocyte proliferation and apoptosis. Results were correlated with clinical severity of the disease, assessed by psoriatic area and severity index (PASI) score. 15 biopsies of normal skin were included as control. Results: Dermal inflammation, Ki-67%, and p53% were significantly higher in psoriatic skin lesion than in normal skin. Those changes were significantly correlated with PASI score. Multiple logistic regression models revealed that Ki-67 was the most significant variable contributing to clinical severity. One unit change in ki-67% can explain 1.2 unit changes in PASI score with 97% sensitivity, 40% specificity and 25% cut-off value. PASI score, dermal inflammation, Ki-67% and P53% were significantly reduced after methotrexate therapy. No significant difference was detected between the percent reduction in PASI score (81%) and that of Ki-67% (70%). Conclusion: Keratinocyte proliferation was the most significant variable contributing to the clinical severity of psoriasis and it was the single parameter that showed parallel changes to PASI score after methotrexate therapy. Keratinocyte proliferation may be considered as the stimulus that induces all other pathological and clinical changes in psoriasis and should be targeted by therapy.","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"1 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70818927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.5455/JIHP.20161231122553
H. Hussin, F. A. Ghani
{"title":"Ovarian Endometrioid Borderline Tumor Arising From an Endometriotic Cyst","authors":"H. Hussin, F. A. Ghani","doi":"10.5455/JIHP.20161231122553","DOIUrl":"https://doi.org/10.5455/JIHP.20161231122553","url":null,"abstract":"","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"1 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70819255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.5455/JIHP.20161208123939
Reshma G. Kini, H. Choudhury
Hansen’s disease is a chronic infectious condition affecting principally the skin and peripheral nerves [1]. In the absence of the classic involvement of the peripheral nerves, the clinical manifestations of leprosy are diverse and can mimic a host of other skin disorders. With decreasing prevalence of the disease in the current post-elimination era, the opportunities to study this enigmatic disease are fewer whereas the need to sustain a high level of diagnostic expertise is absolutely necessary. The consequence of untreated or undertreated leprosy is not only the permanent and debilitating deformity suffered by these patients but also the risk posed by these individuals who act as reservoir for infection in the community. At this juncture, the histopathological examination of skin biopsies for diagnosis of leprosy and for the categorization of clinically suspected cases plays a pivotal role [2,3].
{"title":"Clinico-pathological Correlation in Diagnosis of Hansen's Disease: A Histopathologist's Perspective","authors":"Reshma G. Kini, H. Choudhury","doi":"10.5455/JIHP.20161208123939","DOIUrl":"https://doi.org/10.5455/JIHP.20161208123939","url":null,"abstract":"Hansen’s disease is a chronic infectious condition affecting principally the skin and peripheral nerves [1]. In the absence of the classic involvement of the peripheral nerves, the clinical manifestations of leprosy are diverse and can mimic a host of other skin disorders. With decreasing prevalence of the disease in the current post-elimination era, the opportunities to study this enigmatic disease are fewer whereas the need to sustain a high level of diagnostic expertise is absolutely necessary. The consequence of untreated or undertreated leprosy is not only the permanent and debilitating deformity suffered by these patients but also the risk posed by these individuals who act as reservoir for infection in the community. At this juncture, the histopathological examination of skin biopsies for diagnosis of leprosy and for the categorization of clinically suspected cases plays a pivotal role [2,3].","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"1 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70819133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.5455/JIHP.20161208124017
O. T. Somade, Damilola M. Ogunberu, T. Fakayode, Adeola Animashaun
Introduction: Raw edible camphor (EC), and as component of herbal infusions are widely used to treat pile, back pain, erectile dysfunction, and as an aphrodisiac especially in preparation for sexual intercourse by men. It has been traced in umbilical cord, blood, fetal, adipose, and other tissues including brain, where it bioaccumulates. Methods: The study, therefore, investigated the possible histopathological changes in brain, heart, and spleen that may result following EC administration in rats. Thirty animals were used for the study and were divided into six groups of five rats each. Group I animals served as normal control, Group II animals served as vehicle control and were orally administered 6 mL/kg corn oil daily for 7 days, while Groups III-VI animals were orally administered 1, 2, 4, and 6 g/kg EC for 7 days daily. Results and Conclusions: Following the administrations of various doses of EC, the histopathological changes seen in the cerebral cortex of the brain include mild submeningeal spongiosis, mild diffuse spongiosis of the parenchyma, a very mild diffuse gliosis, and presences of gitter cells, while in hippocampus, there were mild diffuse gliosis and disruption of the progression of the hippocampal horns, as well as foci of spongiosis around the hippocampal horns, and neuronal cells have open faced nuclei. No effect was seen in heart and spleen except 4 g/kg of EC that revealed moderate diffuse congestion in spleen only. In conclusion, EC may not have any toxic effects on the cardiac and splenic cells, but had toxic effects on the brain hippocampus and cerebral cortex, and may lead to brain cell damage.
{"title":"Edible Camphor-induced Histopathological Changes in Hippocampus and Cerebral Cortex Following Oral Administration into Rats","authors":"O. T. Somade, Damilola M. Ogunberu, T. Fakayode, Adeola Animashaun","doi":"10.5455/JIHP.20161208124017","DOIUrl":"https://doi.org/10.5455/JIHP.20161208124017","url":null,"abstract":"Introduction: Raw edible camphor (EC), and as component of herbal infusions are widely used to treat pile, back pain, erectile dysfunction, and as an aphrodisiac especially in preparation for sexual intercourse by men. It has been traced in umbilical cord, blood, fetal, adipose, and other tissues including brain, where it bioaccumulates. Methods: The study, therefore, investigated the possible histopathological changes in brain, heart, and spleen that may result following EC administration in rats. Thirty animals were used for the study and were divided into six groups of five rats each. Group I animals served as normal control, Group II animals served as vehicle control and were orally administered 6 mL/kg corn oil daily for 7 days, while Groups III-VI animals were orally administered 1, 2, 4, and 6 g/kg EC for 7 days daily. Results and Conclusions: Following the administrations of various doses of EC, the histopathological changes seen in the cerebral cortex of the brain include mild submeningeal spongiosis, mild diffuse spongiosis of the parenchyma, a very mild diffuse gliosis, and presences of gitter cells, while in hippocampus, there were mild diffuse gliosis and disruption of the progression of the hippocampal horns, as well as foci of spongiosis around the hippocampal horns, and neuronal cells have open faced nuclei. No effect was seen in heart and spleen except 4 g/kg of EC that revealed moderate diffuse congestion in spleen only. In conclusion, EC may not have any toxic effects on the cardiac and splenic cells, but had toxic effects on the brain hippocampus and cerebral cortex, and may lead to brain cell damage.","PeriodicalId":91320,"journal":{"name":"Journal of interdisciplinary histopathology","volume":"5 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70819184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.5455/JIHP.20170607073657
Dibyajyoti Boruah
Objective: The main objectives of this study were: To evaluate nuclear major axis (MAJX) and minor axis (MINX), nuclear area (NA), nuclear perimeter (NP), nuclear aspect ratio (NAR), and nuclear roundness (NR) with their variability using morphometric techniques in malignant and benign ovarian tumors and to correlate them with histological grades. Methods: Morphometric parameters were evaluated in 8 low-grade (LG) and 22 high-grade (HG) serous ovarian carcinoma and 30 benign grade (BG) cases by digital image morphometric technique using histological sections. Results: The mean of the size-related nuclear parameters: MAJX, MINX, NA, NP, and their variability were statistically significantly greater (P < 0.01) in malignant cases than benign, whereas mean NAR and its variability were significantly lower in malignant cases. Mean NR was significantly higher but its variability (standard deviation - NR) was significantly lower in malignant cases. Histological grade exhibited strong positive correlation with MAJX ( ρ = 0.864), MINX ( ρ = 0.882), NA ( ρ = 0.875), NP ( ρ = 0.859), and moderate positive correlation with NR ( ρ = 0.682); unlike NAR ( ρ = −0.794). Except NR, all other parameters showed positive correlation with their variability. Mean MINX and NA in HG tumor were statistically significantly higher (P < 0.05). For all malignant cases: MINX > 5.03 µm and NA > 30.44 µm 2 can be used to differentiate from benign with 100% efficiency. Conclusion: Morphometric parameters related to nuclear size and their variability were significantly larger in malignant cases than the benign and showed strong positive correlation with the grades. Nuclear shapes of the malignant nuclei were rounder than the benign. Nuclear morphometry can be gainfully exploited in the diagnosis of ovarian carcinoma quantitatively.
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