Pub Date : 2020-10-28DOI: 10.15406/jmen.2020.08.00305
Alsayid A Aldusogi, K. Enan
Background: Typhoid fever, caused by Salmonella enteric sero-var Typhi remains a public health threat in many countries particularly those with poor sanitary conditions. Ambulatory health care facilities in endemic settings frequently lack laboratory-based diagnostics, resulting in the majority of diagnosis being made clinically and antimicrobials given empirically so we need more developed and specific methods. To detect the causative agents. The objective of this study was to apply Real-time (RT-PCR) for detection of salmonella Typhi among febrile patients at Khartoum state-Sudan. Methods: Blood samples were taken from 100 suspected typhoid cases, they were subjected to conventional blood culture; widal agglutination test and real-time PCR. Blood culture was performed using standard protocol and real time PCR targeting prg K gene. Result: Out of 100 suspected typhoid cases blood culture were positive in 24 cases. The Real-time assay identified 20cases (83%) as positives among the 24 culture Positive cases. However, the assay additionally detected 20 (26%) of cases as Salmonella infection among culture negative patients. Widal test was positive in 16(66.6%). Cases among culture positive cases. However, the test additionally was positive in 44(57.8%) cases among culture negative cases. Conclusions: Our study conclude that PCR Real-time is a rapid, sensitive, and specific test for the diagnosis of typhoid fever especially during antibiotic treatment and/or cultured one in late stages of disease. of Sybr-Green added of specimen DNA. Pure culture DNA Salmonella typhi as positive and Escherichia coli, Acinetobacter baumanni, and pneumoniae used as negative controls.
{"title":"Application of real-time (RT-PCR) for detection of Salmonella Typhi among febrile patients in Khartoum state","authors":"Alsayid A Aldusogi, K. Enan","doi":"10.15406/jmen.2020.08.00305","DOIUrl":"https://doi.org/10.15406/jmen.2020.08.00305","url":null,"abstract":"Background: Typhoid fever, caused by Salmonella enteric sero-var Typhi remains a public health threat in many countries particularly those with poor sanitary conditions. Ambulatory health care facilities in endemic settings frequently lack laboratory-based diagnostics, resulting in the majority of diagnosis being made clinically and antimicrobials given empirically so we need more developed and specific methods. To detect the causative agents. The objective of this study was to apply Real-time (RT-PCR) for detection of salmonella Typhi among febrile patients at Khartoum state-Sudan. Methods: Blood samples were taken from 100 suspected typhoid cases, they were subjected to conventional blood culture; widal agglutination test and real-time PCR. Blood culture was performed using standard protocol and real time PCR targeting prg K gene. Result: Out of 100 suspected typhoid cases blood culture were positive in 24 cases. The Real-time assay identified 20cases (83%) as positives among the 24 culture Positive cases. However, the assay additionally detected 20 (26%) of cases as Salmonella infection among culture negative patients. Widal test was positive in 16(66.6%). Cases among culture positive cases. However, the test additionally was positive in 44(57.8%) cases among culture negative cases. Conclusions: Our study conclude that PCR Real-time is a rapid, sensitive, and specific test for the diagnosis of typhoid fever especially during antibiotic treatment and/or cultured one in late stages of disease. of Sybr-Green added of specimen DNA. Pure culture DNA Salmonella typhi as positive and Escherichia coli, Acinetobacter baumanni, and pneumoniae used as negative controls.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49545330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-12DOI: 10.15406/jmen.2020.08.00304
A. Amro, Hamida Al-Dwibe, Ali Lashhab, Esseid Elzubi, Walid K. Saadawi, Aisha Gashout
Leishmaniasis is vector–borne disease caused by parasitic protozoans belonging to the genus Leishmania and is transmitted by infected phlebotomine sand flies . Sarcoidosis is a multisystemic disorder of unknown cause characterized by the formation of immune granulomas in affected organs. Clinical symptoms, severity and evolution of sarcoidosis are highly heterogeneous and can lead to other diseases with similar clinical and pathologic presentations. In this report we present a case of 77 years-old diabetic Libyan male, with chronic erythematous indurated plaques, and nodules on the face. The patients were treated by multiple physicians with topical and systemic corticosteroids for 25 years without improvement. Microscopic examination of Giemsa stained smears from all lesions showed numerous Leishmania amastigotes in and outside monocytes. Leishmania tropica was identified as causative species. The patient was treated with combination of oral rifampicin (600 mg/day) and isoniazide (300 mg/day) and followed up for 9 months until skin-slit smears and PCR turned negative. In conclusion, CL can be misdiagnosed clinically with any granulomatous skin lesions which are compatible with sarcoidal type granuloma. Molecular diagnosis of CL by implementing Leishmania -specific PCR approaches should be performed routinely in any granulomatous skin lesion.
{"title":"Cutaneous leishmaniasis mimicking sarcoidosis in Libyan patient: A case report","authors":"A. Amro, Hamida Al-Dwibe, Ali Lashhab, Esseid Elzubi, Walid K. Saadawi, Aisha Gashout","doi":"10.15406/jmen.2020.08.00304","DOIUrl":"https://doi.org/10.15406/jmen.2020.08.00304","url":null,"abstract":"Leishmaniasis is vector–borne disease caused by parasitic protozoans belonging to the genus Leishmania and is transmitted by infected phlebotomine sand flies . Sarcoidosis is a multisystemic disorder of unknown cause characterized by the formation of immune granulomas in affected organs. Clinical symptoms, severity and evolution of sarcoidosis are highly heterogeneous and can lead to other diseases with similar clinical and pathologic presentations. In this report we present a case of 77 years-old diabetic Libyan male, with chronic erythematous indurated plaques, and nodules on the face. The patients were treated by multiple physicians with topical and systemic corticosteroids for 25 years without improvement. Microscopic examination of Giemsa stained smears from all lesions showed numerous Leishmania amastigotes in and outside monocytes. Leishmania tropica was identified as causative species. The patient was treated with combination of oral rifampicin (600 mg/day) and isoniazide (300 mg/day) and followed up for 9 months until skin-slit smears and PCR turned negative. In conclusion, CL can be misdiagnosed clinically with any granulomatous skin lesions which are compatible with sarcoidal type granuloma. Molecular diagnosis of CL by implementing Leishmania -specific PCR approaches should be performed routinely in any granulomatous skin lesion.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45426270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-02DOI: 10.15406/jmen.2020.08.00303
M. Hamad, Trig Mohamed Elfaki, Essam Zarrug, Hussein Omer Musa Mohammed, Sally Hassan Mohammed, Randa Alaageb Haj Ahmad, Rabab Mohammed Eltaher, Tasneem Isamaldein Ahamed Karrar
Schistosoma haematobium is a causative agent of urinary Schistosomiasis and Schistosoma mansoni causing intestinal Schistosomiasis which is wide spread worms in the world and are considered a foremost health problem in the Sudan. Cross sectional study was carried out among school aged children in Altakamol area - Khartoum state, from February to March 2017 to recognize the prevalence of Schistosoma haematobium and Schistosoma mansoni among school children in the area. One hundred sixty samples were collected randomly using random systematic manner including, 80samples from male and 80 from female. Forty urine samples and 40 stool samples were collected form student at class 3, 5, 6 and 7 and examined macroscopically and microscopically. The data collected through questionnaire. Of the 160 participants6 infected with Schistosoma hematobium and 2 were infected with Schistosoma mansoni. The overall prevalence of Schistosomiasis in the area was 5%.Significant association was observed between gender and age and acquiring Schistosoma infection (p =0.01). Further study among school children with large sample size is needed.
{"title":"Prevalence of schistosomiasis among school aged children in Altakamol area, Khartoum state, Sudan","authors":"M. Hamad, Trig Mohamed Elfaki, Essam Zarrug, Hussein Omer Musa Mohammed, Sally Hassan Mohammed, Randa Alaageb Haj Ahmad, Rabab Mohammed Eltaher, Tasneem Isamaldein Ahamed Karrar","doi":"10.15406/jmen.2020.08.00303","DOIUrl":"https://doi.org/10.15406/jmen.2020.08.00303","url":null,"abstract":"Schistosoma haematobium is a causative agent of urinary Schistosomiasis and Schistosoma mansoni causing intestinal Schistosomiasis which is wide spread worms in the world and are considered a foremost health problem in the Sudan. Cross sectional study was carried out among school aged children in Altakamol area - Khartoum state, from February to March 2017 to recognize the prevalence of Schistosoma haematobium and Schistosoma mansoni among school children in the area. One hundred sixty samples were collected randomly using random systematic manner including, 80samples from male and 80 from female. Forty urine samples and 40 stool samples were collected form student at class 3, 5, 6 and 7 and examined macroscopically and microscopically. The data collected through questionnaire. Of the 160 participants6 infected with Schistosoma hematobium and 2 were infected with Schistosoma mansoni. The overall prevalence of Schistosomiasis in the area was 5%.Significant association was observed between gender and age and acquiring Schistosoma infection (p =0.01). Further study among school children with large sample size is needed.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42208803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-09-28DOI: 10.15406/JMEN.2020.08.00302
M. Hamad, M. B. Hussein
Schiff bases are the most widely used organic compounds. They have been shown to exhibit a broad range of biological activities, including; antifungal, antibacterial, anti-malarial, anti-proliferative, anti-inflammatory, antiviral, and antipyretic properties. In this study semicarbazone ligand was prepared by condensing 3-nitropenzaldehyde with semicarbazide hydrochloride in 1:1 molar ratio in ethanolic medium. This ligand was used to synthesize metal complexes of copper (II) and nickel (II) in 1:2 molar ratio using ethanol as a solvent .Characterization and structure elucidation of prepared metal complexes have been investigated on the basis of molar conductance and UV and IR spectral studies. The all prepared compounds showed a vital effect against both types of bacteria gram positive (Staphylococcus - aurous ) and gram negative (Escherichia-coli ).
{"title":"Synthesis and antibacterial activity of 3-nitrobenzaldehyde semicarbazone ligand and its Ni (II) and Cu (II) Complexes","authors":"M. Hamad, M. B. Hussein","doi":"10.15406/JMEN.2020.08.00302","DOIUrl":"https://doi.org/10.15406/JMEN.2020.08.00302","url":null,"abstract":"Schiff bases are the most widely used organic compounds. They have been shown to exhibit a broad range of biological activities, including; antifungal, antibacterial, anti-malarial, anti-proliferative, anti-inflammatory, antiviral, and antipyretic properties. In this study semicarbazone ligand was prepared by condensing 3-nitropenzaldehyde with semicarbazide hydrochloride in 1:1 molar ratio in ethanolic medium. This ligand was used to synthesize metal complexes of copper (II) and nickel (II) in 1:2 molar ratio using ethanol as a solvent .Characterization and structure elucidation of prepared metal complexes have been investigated on the basis of molar conductance and UV and IR spectral studies. The all prepared compounds showed a vital effect against both types of bacteria gram positive (Staphylococcus - aurous ) and gram negative (Escherichia-coli ).","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44874430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-08-31DOI: 10.15406/jmen.2020.08.00301
Islam Ahmad, Hiba Sami, Z. Mustafa, Ghazanfar Ali, I. Shukla, A. Raza, H. Khan
The onset of hepatitis A is often sudden and characteristic symptoms are followed, within a few days to a week, by yellowish discoloration of eyes, fever, nausea, vomiting dark urine and jaundice. Serum alanine and aspartate levels of aminotransferase normally all rise rapidly during the prodromal cycle, reach peak rates and then decrease by 75 per cent per week. Serum total bilirubin concentrations reach peak levels later and decline slowly than serum aminotransferases. The period of jaundice persists for <3 weeks in 80% of cases. Nearly all adult patients with clinically evident disease undergo complete clinical restoration with a 5‐month.4
{"title":"Investigation of Hepatitis A virus outbreak in Aligarh and its peripheral areas, Uttar Pradesh, India","authors":"Islam Ahmad, Hiba Sami, Z. Mustafa, Ghazanfar Ali, I. Shukla, A. Raza, H. Khan","doi":"10.15406/jmen.2020.08.00301","DOIUrl":"https://doi.org/10.15406/jmen.2020.08.00301","url":null,"abstract":"The onset of hepatitis A is often sudden and characteristic symptoms are followed, within a few days to a week, by yellowish discoloration of eyes, fever, nausea, vomiting dark urine and jaundice. Serum alanine and aspartate levels of aminotransferase normally all rise rapidly during the prodromal cycle, reach peak rates and then decrease by 75 per cent per week. Serum total bilirubin concentrations reach peak levels later and decline slowly than serum aminotransferases. The period of jaundice persists for <3 weeks in 80% of cases. Nearly all adult patients with clinically evident disease undergo complete clinical restoration with a 5‐month.4","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47619114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-08-31DOI: 10.15406/jmen.2020.08.00300
S. T. Akinwotu, Oluwaseun Fapohunda
Resistance to antimicrobials is a global threat that demands immediate response. The excessive use and misuse of antimicrobials has led to the development of multidrug resistant strains of microorganisms. As the consumption rate of these drugs increases, the resistance rate also increases, resulting in high cost of medical treatment and increased mortality rate. To war against antimicrobial resistance, the biochemical and genetic mechanism of resistance in microorganisms is a key factor to be considered. Recent articles from reputable journals/archives including PubMed, Scopus, Google Scholar, ResearchGate etc were retrieved and studied and we discovered that much have been researched on the importance of drugs in the treatment of antimicrobial resistance and in recent decade researchers have been delving into important botanicals that could address multidrug resistance with several opinions. Researchers have opinionated that phytoconstituents could be potential drugs that would address antimicrobial resistance with little or no side effect and they are relatively cheap and readily available to low-income people. However, the future is largely going to be machine driven and little have been reported on the emerging technologies that could adequately tackle this menace of antimicrobial resistance. Here we succinctly discussed how some of these promising technologies could be employed in combating the resistance of microorganism to antibiotics; the use of vaccine technologies, the use of Artificial Intelligence coupled with machine learning algorithms, the use of nanosystems and the use of CRISPR-cas technology.
{"title":"War against antimicrobial resistance","authors":"S. T. Akinwotu, Oluwaseun Fapohunda","doi":"10.15406/jmen.2020.08.00300","DOIUrl":"https://doi.org/10.15406/jmen.2020.08.00300","url":null,"abstract":"Resistance to antimicrobials is a global threat that demands immediate response. The excessive use and misuse of antimicrobials has led to the development of multidrug resistant strains of microorganisms. As the consumption rate of these drugs increases, the resistance rate also increases, resulting in high cost of medical treatment and increased mortality rate. To war against antimicrobial resistance, the biochemical and genetic mechanism of resistance in microorganisms is a key factor to be considered. Recent articles from reputable journals/archives including PubMed, Scopus, Google Scholar, ResearchGate etc were retrieved and studied and we discovered that much have been researched on the importance of drugs in the treatment of antimicrobial resistance and in recent decade researchers have been delving into important botanicals that could address multidrug resistance with several opinions. Researchers have opinionated that phytoconstituents could be potential drugs that would address antimicrobial resistance with little or no side effect and they are relatively cheap and readily available to low-income people. However, the future is largely going to be machine driven and little have been reported on the emerging technologies that could adequately tackle this menace of antimicrobial resistance. Here we succinctly discussed how some of these promising technologies could be employed in combating the resistance of microorganism to antibiotics; the use of vaccine technologies, the use of Artificial Intelligence coupled with machine learning algorithms, the use of nanosystems and the use of CRISPR-cas technology.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47817155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-08-19DOI: 10.15406/jmen.2020.08.00299
Bahattin Bozdağ, A. Özdemir, Mehmet Hamurcu, C. Özdemir, E. Hakki, S. Gezgin
Bread wheat ( Triticum L.) with a very high economic value and great importance for human consumption is extensively cultivated worldwide. However, the wheat genotypes used experience significant yield loss when exposed to salinity conditions due to the fact that salt is a factor that affects plant metabolism. Nitric oxide, a well-known signalling molecule due to its therapeutic effects on human but produced internally also by plant species, can be utilized to ameliorate the adverse effects of the salinity stress conditions of plants. In this study, the changes caused by external nitric oxide applications on leaves anatomy of two bread wheat genotypes exposed to salinity stress were determined. The results were evaluated statistically by using numerical data obtained from the anatomical measurements.
{"title":"Numerical and statistical evaluation of nitric oxide effect on leaf anatomy of Triticum genotypes under salinity stress","authors":"Bahattin Bozdağ, A. Özdemir, Mehmet Hamurcu, C. Özdemir, E. Hakki, S. Gezgin","doi":"10.15406/jmen.2020.08.00299","DOIUrl":"https://doi.org/10.15406/jmen.2020.08.00299","url":null,"abstract":"Bread wheat ( Triticum L.) with a very high economic value and great importance for human consumption is extensively cultivated worldwide. However, the wheat genotypes used experience significant yield loss when exposed to salinity conditions due to the fact that salt is a factor that affects plant metabolism. Nitric oxide, a well-known signalling molecule due to its therapeutic effects on human but produced internally also by plant species, can be utilized to ameliorate the adverse effects of the salinity stress conditions of plants. In this study, the changes caused by external nitric oxide applications on leaves anatomy of two bread wheat genotypes exposed to salinity stress were determined. The results were evaluated statistically by using numerical data obtained from the anatomical measurements.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44172527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-20DOI: 10.15406/JMEN.2020.08.00298
Abdelhakam G. Tamomh, B. Almugadam, A. Elkhalifa
Novel corona virus disease (COVID-19) was reported in late 2019 in Wuhan city in China. It came in to limelight when a cluster or group of cases for pneumonia were detected and investigated with unknown causes. New cases were identified and detected in other countries, following the outbreak in China and the epidemic rapidly continued to spread globally. Till now, more than thirteen million and several thousand individuals have been infected with COVID-19 with several thousand mortalities. According to the epidemiological view or evidence in many countries, the estimation of the risk associated to the COVID-19 epidemic ranged from moderate risk to high.1-3 The World Health Organization (WHO) on March 11 of 2020 announced the prevalence of the novel corona virus disease is a pandemic. Currently, More than nine million confirmed cases of COVID-19 detected in 198 countries are identified and reported globally.4
{"title":"The low cases reported in Sudan regarding a pandemic COVID-19 and Sudan’s Health system responding","authors":"Abdelhakam G. Tamomh, B. Almugadam, A. Elkhalifa","doi":"10.15406/JMEN.2020.08.00298","DOIUrl":"https://doi.org/10.15406/JMEN.2020.08.00298","url":null,"abstract":"Novel corona virus disease (COVID-19) was reported in late 2019 in Wuhan city in China. It came in to limelight when a cluster or group of cases for pneumonia were detected and investigated with unknown causes. New cases were identified and detected in other countries, following the outbreak in China and the epidemic rapidly continued to spread globally. Till now, more than thirteen million and several thousand individuals have been infected with COVID-19 with several thousand mortalities. According to the epidemiological view or evidence in many countries, the estimation of the risk associated to the COVID-19 epidemic ranged from moderate risk to high.1-3 The World Health Organization (WHO) on March 11 of 2020 announced the prevalence of the novel corona virus disease is a pandemic. Currently, More than nine million confirmed cases of COVID-19 detected in 198 countries are identified and reported globally.4","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43870218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-13DOI: 10.15406/JMEN.2020.08.00297
O. Diarra, Christiane Demble, A. Babana, F. Samaké, Boubacar Madio dit Aladiogo Maiga, K. Maiga, M. Diarra, A. Traoré, D. Ouattara, Djeneba Nantoumé, Ibrahim Mallé
Summary Fishing is a millennial tradition in Mali. It is an important sub-sector of the Malian economy, depending in particular on the Niger River, which is 4,200 km long, including 1,700 km in Mali. Its potential importance of existing resources is expressed in its socio-cultural role, job creation, food security and poverty reduction. Due to their pervasiveness, mycotoxin-producing molds are able to reduce the nutritional value of smoked fish by developing several mycotoxins. Smoked fish contaminated with mycotoxins have harmful effects on human health. Samples of smoked fish for sale were collected in the six communes of the district of Bamako and analyzed to determine the fungal flora as well as the concentration of mycotoxins. The mycoflora study of smoked fish has shown that the concentration of fungi varies between 0.5.10 4 CFU/g to 14.10 4 CFU/g DM. whereas aflatoxins by the ELISA method aflatoxin kits (AF) (RIDASCREEN FAST, R-Biopharm AG) were detected in 60 samples taken. This study indicates the need for a continuous assessment of the mycological state of the smoked fish production chain in order to guarantee consumer safety. SAS software was used for all analyzes and the differences were considered significant when p<0.05.
{"title":"Detection of aflatoxins in smoked fish (Clarias anguillaris) sold in the Bamako District","authors":"O. Diarra, Christiane Demble, A. Babana, F. Samaké, Boubacar Madio dit Aladiogo Maiga, K. Maiga, M. Diarra, A. Traoré, D. Ouattara, Djeneba Nantoumé, Ibrahim Mallé","doi":"10.15406/JMEN.2020.08.00297","DOIUrl":"https://doi.org/10.15406/JMEN.2020.08.00297","url":null,"abstract":"Summary Fishing is a millennial tradition in Mali. It is an important sub-sector of the Malian economy, depending in particular on the Niger River, which is 4,200 km long, including 1,700 km in Mali. Its potential importance of existing resources is expressed in its socio-cultural role, job creation, food security and poverty reduction. Due to their pervasiveness, mycotoxin-producing molds are able to reduce the nutritional value of smoked fish by developing several mycotoxins. Smoked fish contaminated with mycotoxins have harmful effects on human health. Samples of smoked fish for sale were collected in the six communes of the district of Bamako and analyzed to determine the fungal flora as well as the concentration of mycotoxins. The mycoflora study of smoked fish has shown that the concentration of fungi varies between 0.5.10 4 CFU/g to 14.10 4 CFU/g DM. whereas aflatoxins by the ELISA method aflatoxin kits (AF) (RIDASCREEN FAST, R-Biopharm AG) were detected in 60 samples taken. This study indicates the need for a continuous assessment of the mycological state of the smoked fish production chain in order to guarantee consumer safety. SAS software was used for all analyzes and the differences were considered significant when p<0.05.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47894111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-30DOI: 10.15406/JMEN.2020.08.00296
M. Hamad, Waseem Sameer Kwami, Abass A. Ahmed
Introduction: Pulmonary Tuberculosis (TB) and human immunodeficiency virus (HIV) have been closely connected since the appearance of acquired immunodeficiency syndrome (AIDS). HIV infection among active TB cases provides real challenges in diagnosis and treatment of TB. This is descriptive cross-sectional study conducted at Abu-anja Teaching Hospital; Khartoum state in period from October 2018to March 2019.The objective was to determine the prevalence of Human Immunodeficiency Virus infection among tuberculosis patients. Method: A total of 90 venous blood samples were collected from the study participants, serum was obtained by centrifugation at (3000 rpm) for 5 minutes. The levels of HIV antibody titer were determined by using sandwich ELISA Assay. Results: The studies revealed that out of the 90 participants of the tuberculosis patients, 5(5.6%) were HIV positive, and the HIV prevalence among male patients were significantly higher than female patients. Conclusion: The study concluded that there is high prevalence of HIV among tuberculosis patients in Khartoum state, capital of Sudan (5.6%) comparing with (5%) at which the WHO recommended intensified intervention to address TB-HIV co-infection critical in any strategy that aims to reach those most in need.
{"title":"Prevalence of human immunodeficiency virus among tuberculosis patients in Khartoum state","authors":"M. Hamad, Waseem Sameer Kwami, Abass A. Ahmed","doi":"10.15406/JMEN.2020.08.00296","DOIUrl":"https://doi.org/10.15406/JMEN.2020.08.00296","url":null,"abstract":"Introduction: Pulmonary Tuberculosis (TB) and human immunodeficiency virus (HIV) have been closely connected since the appearance of acquired immunodeficiency syndrome (AIDS). HIV infection among active TB cases provides real challenges in diagnosis and treatment of TB. This is descriptive cross-sectional study conducted at Abu-anja Teaching Hospital; Khartoum state in period from October 2018to March 2019.The objective was to determine the prevalence of Human Immunodeficiency Virus infection among tuberculosis patients. Method: A total of 90 venous blood samples were collected from the study participants, serum was obtained by centrifugation at (3000 rpm) for 5 minutes. The levels of HIV antibody titer were determined by using sandwich ELISA Assay. Results: The studies revealed that out of the 90 participants of the tuberculosis patients, 5(5.6%) were HIV positive, and the HIV prevalence among male patients were significantly higher than female patients. Conclusion: The study concluded that there is high prevalence of HIV among tuberculosis patients in Khartoum state, capital of Sudan (5.6%) comparing with (5%) at which the WHO recommended intensified intervention to address TB-HIV co-infection critical in any strategy that aims to reach those most in need.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48187498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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