Pub Date : 2021-07-15DOI: 10.15406/jmen.2021.09.00330
E. Tamayo-Legorreta, Eduardo Moreno-Vázquez
Background: The COVID-19 is potentially severe acute respiratory infectious disease, increasing day by day. The cumulative incidence of coronavirus disease (COVID-19) cases is showing similar trends in the globally confirming that, while at a different stage depending on the country, the COVID-19 pandemic has progressed rapidly in all countries. In this study, we evaluate the cumulative incidence of COVID-19 in 10 cities located in all regions of Mexico. Material and methods: Confirmed COVID-19 cases were obtained from the Government of Mexico’s open data portal. A listing of the metropolitan zones with most cases was built. Results: At the time of the data cut-off point, COVID-19 confirmed cases in the Valle de Mexico metropolitan area are more than 233 000, with a cumulative incidence of 183 cases/100 000 habitants. Even though Valle de Mexico has the highest reported number of cases, it is Villahermosa city which has the highest cumulative incidence with 647 cases/100 000 habitants. Conclusions: The estimation of cases through the Sentinel model has shown that, the real extension of the epidemic could be at least 8 times higher that official data; a situation that would place Mexico as one of the most affected countries in Latin America.
{"title":"Description of the cumulative incidence of COVID-19 in the 10 cities of Mexico with most accumulated cases after 120 days of the first confirmed case","authors":"E. Tamayo-Legorreta, Eduardo Moreno-Vázquez","doi":"10.15406/jmen.2021.09.00330","DOIUrl":"https://doi.org/10.15406/jmen.2021.09.00330","url":null,"abstract":"Background: The COVID-19 is potentially severe acute respiratory infectious disease, increasing day by day. The cumulative incidence of coronavirus disease (COVID-19) cases is showing similar trends in the globally confirming that, while at a different stage depending on the country, the COVID-19 pandemic has progressed rapidly in all countries. In this study, we evaluate the cumulative incidence of COVID-19 in 10 cities located in all regions of Mexico. Material and methods: Confirmed COVID-19 cases were obtained from the Government of Mexico’s open data portal. A listing of the metropolitan zones with most cases was built. Results: At the time of the data cut-off point, COVID-19 confirmed cases in the Valle de Mexico metropolitan area are more than 233 000, with a cumulative incidence of 183 cases/100 000 habitants. Even though Valle de Mexico has the highest reported number of cases, it is Villahermosa city which has the highest cumulative incidence with 647 cases/100 000 habitants. Conclusions: The estimation of cases through the Sentinel model has shown that, the real extension of the epidemic could be at least 8 times higher that official data; a situation that would place Mexico as one of the most affected countries in Latin America.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43257975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-25DOI: 10.15406/jmen.2021.09.00329
H. Anan
{"title":"Paleontology, stratigraphy, paleoenvironment and paleogeography of the seventy Tethyan Maastrichtian-Paleogene foraminiferal species of Anan, a review","authors":"H. Anan","doi":"10.15406/jmen.2021.09.00329","DOIUrl":"https://doi.org/10.15406/jmen.2021.09.00329","url":null,"abstract":"","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45321772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-07DOI: 10.15406/jmen.2021.09.00328
F. Nacinovich, A. Fiori, P FernandezOses, S. Castillo, M. Pennini, M. Merkt, I. Chapman, J. Costabel, R. Ronderos, F. Piccinini, D. Navia
{"title":"Rhodococcus erythropolis prosthetic valve infective endocarditis: case report","authors":"F. Nacinovich, A. Fiori, P FernandezOses, S. Castillo, M. Pennini, M. Merkt, I. Chapman, J. Costabel, R. Ronderos, F. Piccinini, D. Navia","doi":"10.15406/jmen.2021.09.00328","DOIUrl":"https://doi.org/10.15406/jmen.2021.09.00328","url":null,"abstract":"","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43184413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-07DOI: 10.15406/jmen.2021.09.00327
Nguyen Anh Quang, N. N. Y. Nhi, Le Ha Duc Anh
Multi–drug resistant Pseudomonas aeruginosa , a significant pathogen threats the public health with high mortality. The potential of phage cocktail was designed to lysis various bacterial sources. The candidate phage was isolated from soil, river water, tap water, food and human stool which belongs to Siphoviridae and Podoviridae family. The results identified that phage cocktails inhibited, lysed multi–drug resistant Pseudomonas aeruginosa in 30 minutes with 3 to 4 log CFU reduction. In addition, these cocktails showed effectiveness to bacterial strains isolated from wide sources including environment, food, and human. This renewed approach is contributed to overcome the dramatical increase of antibiotic resistance.
{"title":"Application of bacteriophage cocktail to control multi-drug resistant Pseudomonas aeruginosa","authors":"Nguyen Anh Quang, N. N. Y. Nhi, Le Ha Duc Anh","doi":"10.15406/jmen.2021.09.00327","DOIUrl":"https://doi.org/10.15406/jmen.2021.09.00327","url":null,"abstract":"Multi–drug resistant Pseudomonas aeruginosa , a significant pathogen threats the public health with high mortality. The potential of phage cocktail was designed to lysis various bacterial sources. The candidate phage was isolated from soil, river water, tap water, food and human stool which belongs to Siphoviridae and Podoviridae family. The results identified that phage cocktails inhibited, lysed multi–drug resistant Pseudomonas aeruginosa in 30 minutes with 3 to 4 log CFU reduction. In addition, these cocktails showed effectiveness to bacterial strains isolated from wide sources including environment, food, and human. This renewed approach is contributed to overcome the dramatical increase of antibiotic resistance.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41670374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-26DOI: 10.15406/jmen.2021.09.00326
M. Ahmed, M. B. Omer, Abduwahab Abakar Fadil, N. Abdelrahman, N. Adam, Marawa Ahmed Mohammed Abd Erahman, Rayan Hassan Sliman, Zeinab Ali Babeker
Pneumonia cases were reported in a hospital in Wuhan, China, on December, 2019.1 After examining throat cultures from patients, the Chinese Center for Disease Control and Prevention stated that these infections were caused by a new form of beta-coronavirus.2 The coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has soon emerged from an epidemic outbreak in Wuhan, China3 into a pandemic that has infected over a million people all over the globe. Attempts of social distancing and the pandemic’s systemic effects concern billions of people. SARS-CoV-2 infects host human cells by binding to the angiotensin-converting enzyme 2 (ACE2) receptor, which is around 80% related to SARS-CoV.3 COVID-19 is primarily characterized as a respiratory tract infection, although new evidence shows that it should be considered a chronic infection affecting multiple systems, including the cardiovascular, respiratory, gastrointestinal, neurological, hematopoietic, and immune systems. Several reports have looked at the impact of COVID-19 on routine hematology variables in a range of countries.4-6 The precise mechanism of disease transmission is uncertain, but recent studies suggest that it is transmitted from person to person. Droplet-based and contact-based transmission routes are thought to be the most probable.7 Serologic investigations are performed on clinical samples such as blood, saliva, or even tears, in addition to molecular detection. Antibodies to SARS-CoV-2, such as IgA, IgM, and IgG, are defined using COVID-19 serologic assays. They’re focused predominantly on enzyme-linked immunosorbent assays, which are less complicated than molecular studies.8 On December 31, 2019, the People’s Republic of China announced a cluster of unidentified pneumonia cases to the World Health Organization (WHO). This outbreak was caused by a new coronavirus categorized as extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that was also accountable for the Corona Virus Disease 2019 (COVID-19). It has had an effect on over 200 countries and territories all over the globe. More than 26 million people have been infected and nearly 1000,000 have died as of mid-May 2020. The aim of this study was to determine the seroprevalence of COVID19 in Khartoum State.
{"title":"Seroprevalence of COVID-19 IgM and IgG antibodies among asymptomatic individuals in Khartoum State-2020","authors":"M. Ahmed, M. B. Omer, Abduwahab Abakar Fadil, N. Abdelrahman, N. Adam, Marawa Ahmed Mohammed Abd Erahman, Rayan Hassan Sliman, Zeinab Ali Babeker","doi":"10.15406/jmen.2021.09.00326","DOIUrl":"https://doi.org/10.15406/jmen.2021.09.00326","url":null,"abstract":"Pneumonia cases were reported in a hospital in Wuhan, China, on December, 2019.1 After examining throat cultures from patients, the Chinese Center for Disease Control and Prevention stated that these infections were caused by a new form of beta-coronavirus.2 The coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has soon emerged from an epidemic outbreak in Wuhan, China3 into a pandemic that has infected over a million people all over the globe. Attempts of social distancing and the pandemic’s systemic effects concern billions of people. SARS-CoV-2 infects host human cells by binding to the angiotensin-converting enzyme 2 (ACE2) receptor, which is around 80% related to SARS-CoV.3 COVID-19 is primarily characterized as a respiratory tract infection, although new evidence shows that it should be considered a chronic infection affecting multiple systems, including the cardiovascular, respiratory, gastrointestinal, neurological, hematopoietic, and immune systems. Several reports have looked at the impact of COVID-19 on routine hematology variables in a range of countries.4-6 The precise mechanism of disease transmission is uncertain, but recent studies suggest that it is transmitted from person to person. Droplet-based and contact-based transmission routes are thought to be the most probable.7 Serologic investigations are performed on clinical samples such as blood, saliva, or even tears, in addition to molecular detection. Antibodies to SARS-CoV-2, such as IgA, IgM, and IgG, are defined using COVID-19 serologic assays. They’re focused predominantly on enzyme-linked immunosorbent assays, which are less complicated than molecular studies.8 On December 31, 2019, the People’s Republic of China announced a cluster of unidentified pneumonia cases to the World Health Organization (WHO). This outbreak was caused by a new coronavirus categorized as extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that was also accountable for the Corona Virus Disease 2019 (COVID-19). It has had an effect on over 200 countries and territories all over the globe. More than 26 million people have been infected and nearly 1000,000 have died as of mid-May 2020. The aim of this study was to determine the seroprevalence of COVID19 in Khartoum State.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42351120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The current study was carried out to evaluate the association between the presence of gastrointestinal ectoparasites and endoparasites with the body condition in sheep sampled in 4 farms located in the municipality of Valledupar, Cesar. The type of study is descriptive, prospective and cross-sectional. Samples were taken from 111 sheep without exclusion of sex distributed in 4 farms in the municipality of Valledupar. The McMaster technique was used to determine the parasite loads of the sheep, later stool cultures were made to the samples that presented high parasite loads, the Baerman technique was applied in order to identify the parasites present in the samples and it was evaluated if there is a relationship between body condition/anemic state, body condition / parasite load and anemic state/ parasite load using the R statistical software and the Chi-square test as hypothesis test. The results show that the prevalence in this research was 84.6% of which 22.5% of the parasitized sheep had high loads, presenting a higher prevalence of the gastrointestinal endoparasite Haemonchus sp with a percentage of 9.9% (11/25), followed by Cooperia with 6.3% (7/25), Ostertagia sp 2.7% (3/25), Trichuris sp 0.9% (1/25) Strongyloides sp 0.9% (1/25), Eimeria 0.9% (1/25) and finally Trichostrongylus with a percentage 0.9% (1/25) for a total of 22.5% and biparasitism did not occur. No ectoparasites were found in sheep. The association between the body anemic status of the sheep determined by the Famacha technique and the presence of gastrointestinal parasites was p<0.05; while the relationship between the body condition in sheep and the parasites presented in them was p>0.05. In conclusion, it is established that if there is an association between Famacha and the presence of gastrointestinal endoparasites, the association between body condition and ovine parasites is not established, taking into account that Famacha turned out to be a useful technique to determine in which anemic state and which the body condition is not associated with the parasite load and is responsible for the fact that this technique is subjective and that therefore the decrease in the body mass and weight of the sheep may be due to various factors and not necessarily to the presence of gastrointestinal endoparasites and ectoparasites , considering that the latter were absent in this research.
{"title":"Association of ectoparasites and gastrointestinal endoparasites and body condition in sheep from four farms of the municipality of Valledupar, Cesar","authors":"Bolano Narvaez Yessika Paola, Canate Gonzalez Abid Silvestre, Herrera Demares Patricia, Bernal Diaz Ana Karina","doi":"10.15406/JMEN.2021.09.00325","DOIUrl":"https://doi.org/10.15406/JMEN.2021.09.00325","url":null,"abstract":"The current study was carried out to evaluate the association between the presence of gastrointestinal ectoparasites and endoparasites with the body condition in sheep sampled in 4 farms located in the municipality of Valledupar, Cesar. The type of study is descriptive, prospective and cross-sectional. Samples were taken from 111 sheep without exclusion of sex distributed in 4 farms in the municipality of Valledupar. The McMaster technique was used to determine the parasite loads of the sheep, later stool cultures were made to the samples that presented high parasite loads, the Baerman technique was applied in order to identify the parasites present in the samples and it was evaluated if there is a relationship between body condition/anemic state, body condition / parasite load and anemic state/ parasite load using the R statistical software and the Chi-square test as hypothesis test. The results show that the prevalence in this research was 84.6% of which 22.5% of the parasitized sheep had high loads, presenting a higher prevalence of the gastrointestinal endoparasite Haemonchus sp with a percentage of 9.9% (11/25), followed by Cooperia with 6.3% (7/25), Ostertagia sp 2.7% (3/25), Trichuris sp 0.9% (1/25) Strongyloides sp 0.9% (1/25), Eimeria 0.9% (1/25) and finally Trichostrongylus with a percentage 0.9% (1/25) for a total of 22.5% and biparasitism did not occur. No ectoparasites were found in sheep. The association between the body anemic status of the sheep determined by the Famacha technique and the presence of gastrointestinal parasites was p<0.05; while the relationship between the body condition in sheep and the parasites presented in them was p>0.05. In conclusion, it is established that if there is an association between Famacha and the presence of gastrointestinal endoparasites, the association between body condition and ovine parasites is not established, taking into account that Famacha turned out to be a useful technique to determine in which anemic state and which the body condition is not associated with the parasite load and is responsible for the fact that this technique is subjective and that therefore the decrease in the body mass and weight of the sheep may be due to various factors and not necessarily to the presence of gastrointestinal endoparasites and ectoparasites , considering that the latter were absent in this research.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43782192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-30DOI: 10.15406/JMEN.2021.09.00322
M. A. Ibrahim, N. Adam, M. A. Adam, N. Abdelrahman, Marawa Ahmed Mohammed Abd Erahman
Background: syphilis is one of the infections transmitted through blood transfusion as shown by high antibodies detection in healthy African blood. Syphilis prevalence among blood donors in sub-Saharan African countries varies among people; In Nigeria, Mali, Tanzania, and Kenya the prevalence was 3.1%, 0.3%, 12.8%, and 3.8% respectively. This study aimed to determine the seroprevalence of syphilis antibodies among blood donors at North Darfur State – Sudan, from 2017 to 2019. Methods: This retrospective descriptive analysis of consecutive blood donor’s data records covering the period from January 2017 to December 2019 was performed. The medical and socio-demographic histories of the donors were registered in the logbook. The data was analyzed using IBM SPSS Statistical package version 20. Prevalence of syphilis was expressed as the number of sero-positive samples per year. Pearson Chi-squire (χ2) test was used to evaluate the relationship between categorical variants. Ethical approval was obtained from Al Fashir University and Sudan Ministry of Health Ethical Review Boards. Results: A total of 14819 blood donors were analyzed. The overall sero-prevalence of syphilis antibodies between 2017 and 2019 were 1927 (13 %). They were all adult’s male, aged between 17 to 64 years, with a median age 29.5 years. The seroprevalence of syphilis was 10.9 % in 2017; increased to 13 % in 2018 and subsequently increased to 14.8 % in 2019. Conclusion: In This study it was observed that the seroprevalence of syphilis among blood donors continues high during the study period.
{"title":"Seroprevalence of syphilis antibodies among blood donors at North Darfur State–Sudan, from 2017 to 2019","authors":"M. A. Ibrahim, N. Adam, M. A. Adam, N. Abdelrahman, Marawa Ahmed Mohammed Abd Erahman","doi":"10.15406/JMEN.2021.09.00322","DOIUrl":"https://doi.org/10.15406/JMEN.2021.09.00322","url":null,"abstract":"Background: syphilis is one of the infections transmitted through blood transfusion as shown by high antibodies detection in healthy African blood. Syphilis prevalence among blood donors in sub-Saharan African countries varies among people; In Nigeria, Mali, Tanzania, and Kenya the prevalence was 3.1%, 0.3%, 12.8%, and 3.8% respectively. This study aimed to determine the seroprevalence of syphilis antibodies among blood donors at North Darfur State – Sudan, from 2017 to 2019. Methods: This retrospective descriptive analysis of consecutive blood donor’s data records covering the period from January 2017 to December 2019 was performed. The medical and socio-demographic histories of the donors were registered in the logbook. The data was analyzed using IBM SPSS Statistical package version 20. Prevalence of syphilis was expressed as the number of sero-positive samples per year. Pearson Chi-squire (χ2) test was used to evaluate the relationship between categorical variants. Ethical approval was obtained from Al Fashir University and Sudan Ministry of Health Ethical Review Boards. Results: A total of 14819 blood donors were analyzed. The overall sero-prevalence of syphilis antibodies between 2017 and 2019 were 1927 (13 %). They were all adult’s male, aged between 17 to 64 years, with a median age 29.5 years. The seroprevalence of syphilis was 10.9 % in 2017; increased to 13 % in 2018 and subsequently increased to 14.8 % in 2019. Conclusion: In This study it was observed that the seroprevalence of syphilis among blood donors continues high during the study period.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43212574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-30DOI: 10.15406/JMEN.2021.09.00323
Osuji Malachy Ikeokwu
This research work was carried out to measure the effect of storage container on the bacterial population of water from different sources over a long storage time. Spread plate and other bacteriological techniques were adopted using Nutrient (NA), Salmonella/ Shigella(SSA), MacConkey(MAC), and Thiosulphite citrate bile sucrose (TCBS) agar. Calabash, Glass, Metal and Plastic containers were used to store rain, river, and tap water samples. Sample analysis was taken at time interval of week 0, 1, 3, 5, and 14. In rainwater stored in calabash, total heterotrophic bioload decreased from 9.2 logcfu/ml at week 0 to 5.3 log cfu/ml at week 5 and steady thereafter. Other bioloads in rain water stored in other containers showed similar trend in growth pattern. For river water stored in calabash container, total heterotrophic bioload decreased all through. For river water stored in glass container, it also decreased from 7.0 logcfu/ml at week 0 to 5.8 logcfu/ml at week 1 and was steady thereafter. Other bioloads in river water stored in other containers decreased from week 0 to week 14 at various concentrations. In tap water stored in glass and other containers, all decreased throughout. Several other factors were discovered in this research work to be responsible for the bioload population decrease observed. They include toxins produced during stationary phase as secondary metabolites, acids, nutrient depletion, pH variation, temperature changes. On the statistical analysis (ANOVA), it showed that the containers have significant effect on the bioload of the stored water after 14 weeks storage as plastic recorded the highest bioload change. To this effect people are advised to use plastic container for water storage for a long period. Also people are to filter the water to remove biofilms formed and debris of bacteria that died due to toxins produced. Nutrient depletion and effect of other factors contributed in bacterial population decrease.
{"title":"Effect of storage container on the bacteriological quality of water from different sources","authors":"Osuji Malachy Ikeokwu","doi":"10.15406/JMEN.2021.09.00323","DOIUrl":"https://doi.org/10.15406/JMEN.2021.09.00323","url":null,"abstract":"This research work was carried out to measure the effect of storage container on the bacterial population of water from different sources over a long storage time. Spread plate and other bacteriological techniques were adopted using Nutrient (NA), Salmonella/ Shigella(SSA), MacConkey(MAC), and Thiosulphite citrate bile sucrose (TCBS) agar. Calabash, Glass, Metal and Plastic containers were used to store rain, river, and tap water samples. Sample analysis was taken at time interval of week 0, 1, 3, 5, and 14. In rainwater stored in calabash, total heterotrophic bioload decreased from 9.2 logcfu/ml at week 0 to 5.3 log cfu/ml at week 5 and steady thereafter. Other bioloads in rain water stored in other containers showed similar trend in growth pattern. For river water stored in calabash container, total heterotrophic bioload decreased all through. For river water stored in glass container, it also decreased from 7.0 logcfu/ml at week 0 to 5.8 logcfu/ml at week 1 and was steady thereafter. Other bioloads in river water stored in other containers decreased from week 0 to week 14 at various concentrations. In tap water stored in glass and other containers, all decreased throughout. Several other factors were discovered in this research work to be responsible for the bioload population decrease observed. They include toxins produced during stationary phase as secondary metabolites, acids, nutrient depletion, pH variation, temperature changes. On the statistical analysis (ANOVA), it showed that the containers have significant effect on the bioload of the stored water after 14 weeks storage as plastic recorded the highest bioload change. To this effect people are advised to use plastic container for water storage for a long period. Also people are to filter the water to remove biofilms formed and debris of bacteria that died due to toxins produced. Nutrient depletion and effect of other factors contributed in bacterial population decrease.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46605042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-18DOI: 10.15406/JMEN.2021.09.00320
A. Portillo-López, Sophia González-Martínez
Marine fungi are essential as recyclers of organic matter in the ocean, as well their secondary metabolites are now studied as potential drugs for different diseases. Despite Mexico having an extensive coastline, few resources have been allocated to the research of this group. Through a thorough review of scientific literature between 2001 and February 2021, a systematic listing of marine fungi on Mexico’s marine waters was constructed. In this work, two orders, forty-nine genera, and thirteen species are recorded, of which 50 are new records. The most frequent phylum was Ascomycota (92%; 50 genera), followed Chytridiomycota (4%; 2 genera) and Basidiomycota (4%; 2 genera). Most of them have been reported in the Gulf of Mexico, followed by the Pacific Ocean, Gulf of California and, the Caribbean. A new halophile species isolated from deep sediment in the Gulf of California (Aspergillus loretoensis) is also reported.
{"title":"Marine fungi from different habitats recorded from 2001 to date in Mexico","authors":"A. Portillo-López, Sophia González-Martínez","doi":"10.15406/JMEN.2021.09.00320","DOIUrl":"https://doi.org/10.15406/JMEN.2021.09.00320","url":null,"abstract":"Marine fungi are essential as recyclers of organic matter in the ocean, as well their secondary metabolites are now studied as potential drugs for different diseases. Despite Mexico having an extensive coastline, few resources have been allocated to the research of this group. Through a thorough review of scientific literature between 2001 and February 2021, a systematic listing of marine fungi on Mexico’s marine waters was constructed. In this work, two orders, forty-nine genera, and thirteen species are recorded, of which 50 are new records. The most frequent phylum was Ascomycota (92%; 50 genera), followed Chytridiomycota (4%; 2 genera) and Basidiomycota (4%; 2 genera). Most of them have been reported in the Gulf of Mexico, followed by the Pacific Ocean, Gulf of California and, the Caribbean. A new halophile species isolated from deep sediment in the Gulf of California (Aspergillus loretoensis) is also reported.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43622125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-12DOI: 10.15406/JMEN.2021.09.00319
J. Belkhair, I. Karrati, M. Tarmidi, M. Mezouari, R. Moutaj
to objectify the number of subjects infested by this parasite in our population. This is a retrospective study carried out over a period of ten years, from January 2009 until December 2018, in the parasitology-mycology laboratory of the Avicenna military hospital in Marrakech. 13,255 subjects were included with at least one parasitological examination of the stool, of which 2,799 examinations were positive, with a simple parasite index of 21.11%. Blastocystis hominis was found in 675 subjects with a specific parasite index of 5.09%, and it was co-associated in 446 subjects with a specific parasite index (IPSp) of 3.36%. The most frequent association was that of Blastocystis hominis and E. histolytica/dispar with a rate of (25.5%) of all polyparasitic patients followed by the combination of Blastocystis hominis and Entamoeba coli (20.8%), the combination of Blastocystis hominis and Endolimax nana with a rate of (16, 1%) and lastly Blastocystis hominis and Entamoeba hartmani (5.3% In patients with polyparasitism, the association of Blastocystis hominis with Entamoeba coli was 20.8%, with Endolimax nana with a rate of 16.1% and finally with Entamoeba hartmani with a prevalence of 5.3%. It is always necessary to insist on strict observance of the rules of prophylaxis against oral-fecal contamination.
{"title":"Blastocystis hominis microbiota: study of 13255 patients and review of the literature ","authors":"J. Belkhair, I. Karrati, M. Tarmidi, M. Mezouari, R. Moutaj","doi":"10.15406/JMEN.2021.09.00319","DOIUrl":"https://doi.org/10.15406/JMEN.2021.09.00319","url":null,"abstract":"to objectify the number of subjects infested by this parasite in our population. This is a retrospective study carried out over a period of ten years, from January 2009 until December 2018, in the parasitology-mycology laboratory of the Avicenna military hospital in Marrakech. 13,255 subjects were included with at least one parasitological examination of the stool, of which 2,799 examinations were positive, with a simple parasite index of 21.11%. Blastocystis hominis was found in 675 subjects with a specific parasite index of 5.09%, and it was co-associated in 446 subjects with a specific parasite index (IPSp) of 3.36%. The most frequent association was that of Blastocystis hominis and E. histolytica/dispar with a rate of (25.5%) of all polyparasitic patients followed by the combination of Blastocystis hominis and Entamoeba coli (20.8%), the combination of Blastocystis hominis and Endolimax nana with a rate of (16, 1%) and lastly Blastocystis hominis and Entamoeba hartmani (5.3% In patients with polyparasitism, the association of Blastocystis hominis with Entamoeba coli was 20.8%, with Endolimax nana with a rate of 16.1% and finally with Entamoeba hartmani with a prevalence of 5.3%. It is always necessary to insist on strict observance of the rules of prophylaxis against oral-fecal contamination.","PeriodicalId":91326,"journal":{"name":"Journal of microbiology & experimentation","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48573028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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