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Viability test exclusively is not adequate to evaluate the T4 Acanthamoeba keratitis’ treatment 单纯的活力试验不足以评价T4棘阿米巴角膜炎的治疗效果
Pub Date : 2020-01-08 DOI: 10.15406/JMEN.2020.08.00278
Daniella de Sousa Mendes Moreira Alves, G. Bernardes, Daianny Costa da Silva, Luciano Moreira Alves, A. M. Castro, M. C. Vinaud
Acanthamoeba keratitis is a serious corneal infection and in some cases can lead to blindness.1 The T4 genotype is often associated with human infections by Acanthamoeba.2,3 The incidence of keratitis has increased considerably worldwide, especially those associated to contact lenses use.4,5 Cysts and recurrence of infection represent a challenge in the treatment of keratitis due to its high resistance to adverse conditions and to most medications.6,7 This intriguing property can lead to late diagnosis and can explain absence of cyst removal during and after therapy regimen.8 In early stage, due to symptomatology and incorrect diagnosis the disease can be inappropriately mistaken for keratitis by other microorganisms, such as fungi and bacteria, leading to treatment with antifungals or antibiotics that will not be effective. Therefore, delaying proper treatment for this infection can increase severity and risk of blindness.9
棘阿米巴角膜炎是一种严重的角膜感染,在某些情况下可导致失明T4基因型通常与棘阿米巴的人类感染有关。2,3角膜炎的发病率在世界范围内显著增加,特别是与使用隐形眼镜有关的。4,5由于角膜炎对不利条件和大多数药物具有很高的耐药性,因此囊肿和感染复发是角膜炎治疗中的一个挑战。这一有趣的特性可能导致晚期诊断,并可以解释在治疗期间和治疗后没有囊肿切除的原因在早期,由于症状和不正确的诊断,疾病可能被其他微生物(如真菌和细菌)错误地误认为角膜炎,导致使用抗真菌药物或抗生素治疗无效。因此,延迟对这种感染的适当治疗可能会增加严重程度和失明的风险
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引用次数: 1
Spondylodiscitis due to Brucella spp. case report  布鲁氏菌引起的脊柱炎一例报告
Pub Date : 2019-12-05 DOI: 10.15406/jmen.2019.07.00276
Alvarez Delgado Karla Selene, Martinez Navarro Jovana
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引用次数: 1
Susceptibility of the deviants 离经叛道者的敏感性
Pub Date : 2019-11-29 DOI: 10.15406/jmen.2019.07.00275
Grishma V. Kulkarni
Genetic variability is essential to occur evolution. The fitness of the organism depends on its capacity to adapt to the changing environmental conditions. Antimicrobial agents exert strong selective pressure on the bacterial population favoring the microorganisms that are capable of resisting them. Genetic variability may occur by variety of the mechanisms such as micro evolutionary changes in the form of point mutations and macro evolutionary changes in the large segment of the DNA which ultimately leads to acquired resistance and better survival of the organisms.1 Intrinsic resistance is also not an exception to this transformation as documented by HHS studies about Burkholderia pseudomallei and other Minimum inhibitory concentration (MIC) studies as well. Though B.pseuodomallei is known for its intrinsic resistance to aminoglycosides and macrolide, found to be susceptible to them due absence of or nonfunctional RND (resistance nodulation division) pumps.
遗传变异性对进化至关重要。生物体的适应性取决于它适应不断变化的环境条件的能力。抗菌剂对细菌种群施加强大的选择性压力,有利于抵抗它们的微生物。遗传变异可能通过多种机制发生,如点突变形式的微观进化变化和DNA大片段的宏观进化变化,最终导致获得性耐药性和生物体更好的生存。1正如卫生和公众服务部关于伯克霍尔德菌的研究所记录的那样,内在耐药性也不是这种转化的例外pseudomalli和其他最小抑制浓度(MIC)研究。尽管B.pseuodomallei以其对氨基糖苷类和大环内酯类的内在耐药性而闻名,但由于缺乏或无功能的RND(抗性结瘤分裂)泵,它对它们很敏感。
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引用次数: 0
Rapid method for determining the antimicrobial susceptibility on chromogenic agar for urine specimen 尿标本显色琼脂药敏快速测定方法
Pub Date : 2019-11-15 DOI: 10.15406/jmen.2019.07.00272
Renata Lazo de la Vega Giraud, Estefania Guadalupe Alvarado Bravo, Gabriel Chavez Giraud, C. Rodríguez
The traditional basis for the evaluation of urinary tract pathogens is the urine culture and the antibiotic susceptibility testing. The major drawback of the current microbiology approach is the time lapse of 2-3 days between specimen collection and the availability of the result with an objective evidence for treatment selection. Antibiotic resistance is a significant problem not only among nosocomial complicated UTIs, where it has traditionally been recognized, but also in community acquired simple UTIs. Early microbiological diagnosis and the correct administration of the appropriate initial antimicrobial therapy have proven to be associated with decrease rates of morbidity. Direct susceptibility testing of blood culture is being used in some microbiology laboratories, methods for urine cultures have been evaluated, some do not recommend the procedure, and others advocate a variety of conditions for acceptance of results. We performed a comparison between rapid method and standardized disk diffusion method in urine specimen, determining the antimicrobial susceptibility on CHROMagar Orientation Medium, without having to wait for bacterial isolation, our method may provide preliminary information that improves antimicrobial use and it also has a very good correlation with standard procedures. Methods
传统的评价尿路病原菌的依据是尿培养和药敏试验。当前微生物学方法的主要缺点是标本收集和获得治疗选择的客观证据之间的时间间隔为2-3天。抗生素耐药性不仅是传统上公认的院内复杂性尿路感染的重要问题,而且在社区获得性单纯性尿路感染中也是如此。早期微生物学诊断和正确给予适当的初始抗菌治疗已被证明与发病率降低有关。一些微生物实验室正在采用血培养的直接药敏试验,尿液培养的方法也得到了评价,有的不推荐这种方法,有的则主张有各种接受结果的条件。在CHROMagar取向培养基上对尿标本进行快速法和标准化纸片扩散法的比较,无需等待细菌分离,即可测定尿标本的抗菌药物敏感性,为提高抗菌药物的使用提供初步信息,且与标准程序具有很好的相关性。方法
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引用次数: 0
Bacteriological and physicochemical analyses of well water used for drinking in Ekpoma-Edo State, Nigeria 尼日利亚埃克波马-埃多州饮用井水的细菌学和理化分析
Pub Date : 2019-11-14 DOI: 10.15406/jmen.2019.07.00271
Osatohanmwen Osarenmwinda, Aruya Graceann Idaehor
Portable water is a key requirement for human, whether it is intended for drinking, recreational activities and other domestic purposes. It is a vital desire for all life forms. It is therefore imperative that adequate amount of portable, clean and safe water be made available to other life forms such as flora and fauna. Inadequate quantity of it results to mobility and fatality rate in rural settlements where chemical contaminants and water-based infections are endemic and persistent because of poor groundwater and surface waters quality.1 The global health importance of water quality is a concept that needs not to be neglected as quite a number of infectious diseases are contracted by water via faecal-oral mode of transmission. These infections have been reported of having a fatality rate of 5 million children annually, causing 1/6th of the world population ill.2 Water borne infections emanate from intake of untreated contaminated water by pathogenic microbes. These infections are linked with the non-availability and accessibility to clean, portable water supply in addition to unhygienic vicinity. This affects man and the biotic components of the ecosystem especially in developing countries. The following bacteria genera are often incriminated in water based infections; Pseudomonas aeruginosa, Shigella sonnei, Salmonella, Klebsiella, lyanobacteria, Proteus, Vibrio, Mycobacteria, Streptococcus faecalis e.t.c.3 Fresh water which lies below the earth crust in broken segments of rocks and soil pore spaces is considered as groundwater. It is often regarded as an ideal source of water because it is seem not to be opaque and clean. This is attributed to its passage via various layers and sediments of rocks, which act as a sort of natural filtration system. However it portability and quality can be compromise as a result of poor source protection and resource management.4 There is an increase in the spite of ground water contamination especially in urban settlements with variety of industrial activities, increase in the number of inhabitants, poor hygiene, use of land for mechanized and commercial farming and indiscriminate disposal of wastes on land.5 The presence of contaminants whether inorganic or organic in the ground water above maximum limits sets by water regulatory agencies such as WHO, EPA, NIS and FEPA may cause a serious health calamity.6 Inhabitants of developing countries unavoidably still rely on contaminated ground water due to non availability of potable water sources.7 Water apart from its domestic applications has various other aspect of use such as transportation, generation of hydro-power electricity, irrigation and aquaculture. It is a major driving force that controls the evolution and functionality of the universe on earth.8 Varieties of artificial chemicals pollutants such as insecticides, pesticides, nitrates from fertilizers, sulphates, chlorides, phenols, soap and heavy metals e.t.c are chief contributors to water contaminat
无论是用于饮用、娱乐活动还是其他家庭用途,饮用水都是人类的关键需求。它是对所有生命形式的重要渴望。因此,必须向动植物等其他生命形式提供足够数量的便携式、清洁和安全的水。由于地下水和地表水质量差,化学污染物和水性感染是地方性和持久性的,因此数量不足会导致农村住区的流动性和死亡率。1水质对全球健康的重要性是一个不容忽视的概念,因为相当多的传染病是通过粪口传播的传输。据报道,这些感染每年有500万儿童死亡,导致世界六分之一的人口患病。2水传播感染源于病原微生物摄入未经处理的污染水。除了附近不卫生外,这些感染还与无法获得清洁的便携式供水有关。这影响到人类和生态系统中的生物组成部分,尤其是在发展中国家。以下细菌属通常在基于水的感染中被定罪;铜绿假单胞菌、宋尼志贺菌、沙门氏菌、克雷伯氏菌、裂杆菌、变形杆菌、弧菌、分枝杆菌、粪链球菌e.t.c.3位于地壳下岩石和土壤孔隙破碎部分的淡水被视为地下水。它通常被认为是一个理想的水源,因为它似乎不是不透明和干净的。这归因于它通过各种岩石层和沉积物,这些岩石层和沉积起到了一种天然过滤系统的作用。然而,由于水源保护和资源管理不善,地下水的可携带性和质量可能会受到影响。4尽管地下水污染有所增加,尤其是在工业活动多样、居民人数增加、卫生条件差的城市住区,将土地用于机械化和商业化农业以及不分青红皂白地处理土地上的废物。5地下水中无机或有机污染物的存在高于世界卫生组织、环境保护局、,NIS和FEPA可能会造成严重的健康灾难。6由于缺乏饮用水源,发展中国家的居民不可避免地仍然依赖受污染的地下水。7水除了在国内应用外,还有其他各种用途,如运输、水力发电、灌溉和水产养殖。它是控制地球上宇宙进化和功能的主要驱动力。8各种人工化学污染物,如杀虫剂、杀虫剂、化肥中的硝酸盐、硫酸盐、氯化物、酚类、肥皂和重金属等,是造成水污染的主要原因。镉、砷、铅、锌、铁、铜和锰是重金属中严重而危险的污染物。8水是我们日常生活的基本需求,因此需要对其进行彻底的物理化学和细菌调查。因此,本研究旨在确定埃克波马的饮用井水质量,尼日利亚埃多州根据检查其是否符合世界卫生组织和NIS等水监管机构制定的标准,并确定其他地区污染的可能原因,以提出有效建议
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引用次数: 1
Maastrichtian-paleogene benthic foraminifera from the Middle East and its distribution in the Tethys, a review 中东马斯特里赫特-古近系底栖有孔虫及其在特提斯的分布综述
Pub Date : 2019-11-11 DOI: 10.15406/jmen.2019.07.00270
H. Anan
The present paper aims to highlight the paleontology, stratigraphy paleogeography, paleoecology and biogeography of the 119 Maastrichtian-early Paleogene calcareous benthic foraminiferal species and subspecies which were originally erected by many authors from six Middle East countries (Maps 1 & 2): (1) Egypt: Nakkady,1-3 LeRoy,4 Said & Kenawy,5 Abdou & Abdel Kireem,6 Anan,7‒27 Aly et al.,28 (2) Jordan: Futyan,29 (3) Saudi Arabia: Hasson30 (4) Qatar: Hewaidy & Al-Hitmi31 (5) UAE: Anan32‒37 and (6) Pakistan: Haque,38‒40 Nomura & Brohi,41 and many studies are recorded some of these taxa in a wider geographic areas in the Tethys, from Atlantic to Indian Oceans via Mediterranean (Map 3).
本文重点介绍了中东6个国家(图1和图2)的作者首次建立的119种马岛-早古近纪钙质底栖有孔虫的古生物学、地层古地理、古生态和生物地理:(1)埃及:Nakkady,1-3 LeRoy,4 Said & Kenawy,5 Abdou & Abdel Kireem,6 Anan, 7-27 Aly等人,28(2)约旦:Futyan,29(3)沙特阿拉伯:Hasson30(4)卡塔尔:Hewaidy & al - hitmi31(5)阿联酋:Anan32-37和(6)巴基斯坦:Haque, 38-40 Nomura & Brohi,41和许多研究在特提斯更广泛的地理区域记录了这些分类群,从大西洋到印度洋经地中海(地图3)。
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引用次数: 0
First report of Kytococcus schroeteri prosthetic valve endocarditis in Oman 阿曼首例猪粪球球菌人工瓣膜心内膜炎报告
Pub Date : 2019-10-15 DOI: 10.15406/jmen.2019.07.00268
Wafa Nasser Al Tamtami, F. Al Yaquobi, A. Al Jardani, M. Al Riyami, M. Cormican
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引用次数: 2
Characterizing filamentous fungi from Puerto Rico for the bioremediation of organic compounds 波多黎各丝状真菌对有机化合物的生物修复特性
Pub Date : 2019-10-02 DOI: 10.15406/jmen.2019.07.00266
R. Giles
We developed more difficult examination criteria (by the fungi’s perspective) by evaluating the fungal isolates’ ability to grow in minimal media with model toxins as their sole carbon source. We grew isolates on Petri plates containing minimal media plus either 0.02% v/v toluene or 0.02% v/v chlorobenzene. Surprisingly, we observed that several of our isolates exhibited healthy colony growth suggesting they were degrading the compounds as a sole carbon and micronutrient source.
我们通过评估真菌分离株在以模型毒素为唯一碳源的最小培养基中生长的能力,制定了更困难的检查标准(从真菌的角度来看)。我们在含有最小培养基和0.02%v/v甲苯或0.02%v/v氯苯的培养皿上培养分离物。令人惊讶的是,我们观察到我们的几个分离株表现出健康的菌落生长,这表明它们正在降解作为唯一碳和微量营养素来源的化合物。
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引用次数: 0
Sero-epidemiology of Plasmodium vivax infection among Sudanese's and Ethiopians in Khartoum State 喀土穆州苏丹人和埃塞俄比亚人间日疟原虫感染的血清流行病学研究
Pub Date : 2019-09-26 DOI: 10.15406/jmen.2019.07.00265
M. Hamad, Mohanad E Alfadol, M. M. Eltayeb
Plasmodium vivax is a hemo-pathogenic parasite that belongs for the phylum Apicomplexa. It causes malaria which is the public health significant. Most of the research and published literature on malaria focuses on Plasmodium falciparum and much less on Plasmodium vivax.1 This focus is due to the very high burden of mortality attributed to the falciparum species in Africa.2 However, there is growing evidence that Plasmodium vivax is responsible for a significant burden of disease worldwide accounting for half of all malaria cases in Asia and Latin America, will nearly 2.5 billion people at risk of infection.3 All species are transmitted by the bite of an infective female Anopheles mosquitoes control and management of vivax malaria is difficult, and the present particular challenges.4 Hematological changes that have been reported to accompany malaria are anemia, thrombocytopenia, leucopenia, atypical lymphocytosis, and infrequently disseminated intravascular coagulation.5 Some studies have shown that Plasmodium vivax can also cause severe disease and the hematological parameters are usually changed in such cases. The present study was undertaken to assess the hematological alterations in vivax malaria patients in comparison between Sudanese and Ethiopians.6
间日疟原虫是一种血液致病性寄生虫,属于顶复合体门。它会引起疟疾,这对公共卫生有重大影响。大多数关于疟疾的研究和发表的文献都集中在恶性疟原虫上,而对间日疟原虫的研究较少这一重点是由于非洲恶性疟原虫造成了很高的死亡率负担。2然而,越来越多的证据表明,间日疟原虫是造成世界范围内重大疾病负担的罪魁祸首,占亚洲和拉丁美洲所有疟疾病例的一半,将有近25亿人面临感染风险所有种类的疟蚊都是通过雌性疟蚊的叮咬传播的,间日疟的控制和管理是困难的,目前尤其具有挑战性据报道,伴随疟疾的血液学变化包括贫血、血小板减少、白细胞减少、非典型淋巴细胞增多和罕见的弥散性血管内凝血一些研究表明间日疟原虫也可引起严重疾病,在这种情况下通常会改变血液学参数。本研究的目的是比较苏丹人和埃塞俄比亚人间日疟疾患者的血液学变化
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引用次数: 0
Unusual cause of hyponatremia in a young patient with varicella zoster infection: a case report 水痘带状疱疹感染的年轻患者低钠血症的不寻常原因:1例报告
Pub Date : 2019-09-05 DOI: 10.15406/jmen.2019.07.00263
Juhi Chandwani, S. Kantor, Seif S Al Abri, Antara Gokhale, Sadanandan Prakash
Hyponatremia, defined as serum sodium below 135mEq/L, is one of the most frequently encountered electrolyte disorders in critically ill patients.1 Patients at risk include septic patients, postoperative patients, older patients on thiazide diuretics, patients with malignant or psychiatric illness, and endurance athletes. A serum sodium concentration <100mEq/l, carries a high risk of brain damage due to seizure activity. There is concern about injury to the brain for rapid and over correction of hyponatremia. We present here a patient who presented with Varicella Zoster infection and symptomatic hyponatremia due to volitional intake of free water as per advice of homeopathy physician. Hypertonic saline, fluid restriction and close monitoring in the intensive care unit led to complete recovery of the patient with no residual neurological deficit. Case report
低钠血症定义为血清钠低于135mEq/L,是危重患者最常见的电解质紊乱之一有危险的患者包括脓毒症患者、术后患者、服用噻嗪类利尿剂的老年患者、恶性或精神疾病患者以及耐力运动员。血清钠浓度<100mEq/l时,由于癫痫活动导致脑损伤的风险较高。对低钠血症的快速矫治和过度矫治有可能对大脑造成损伤。根据顺势疗法医生的建议,我们在这里报告了一例水痘带状疱疹感染和症状性低钠血症,原因是自愿摄入游离水。高渗生理盐水,限制液体和在重症监护室密切监测导致患者完全恢复,没有残留的神经功能障碍。病例报告
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引用次数: 0
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Journal of microbiology & experimentation
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