Cardiovascular disease (CVD) is the world’s premier cause of morbidity and death. CVD is a class of heart or blood vessel diseases. CVD contains the coronary artery disease (CAD) such as unstable angina (UA) and myocardial infarction (MI) diseases. Clinicians use additional tools to support clinical evaluation and improve their ability to detect the susceptible patient at threat for CVD. Biomarkers are one such method to identify potential risk persons, rapidly and reliably diagnose disease symptoms that efficiently predict and treat disease. Discovery of MicroRNAs (miRNAs) representing a class of small, non-coding RNA molecules opens interesting opportunities to use the patterns of miRNAs as a biomarker for cardiovascular diseases. The objective of this study is to define miRNA and genes potentially associated with MI. Rothman dataset includes 52 samples of Acute Coronary Syndromes (ACS). including 18 patients with myocardial infarction (MI) and 8 patients with unstable angina (UA). Overall (number of genes selected) candidate ncRNA biomarkers have been defined and a ncRNA-based classifier has been created to predict MI risk which based on 7 ncRNA expression data using vector support machines SVM and decision tree classifiers. The experimental results obtained through applying these mechanisms on the Rothman dataset. The classification model’s performance is evaluated using the V-fold validation and LOOCV methods. The outcome of this search can be used by the drug designer for pathway analysis and CVD treatment decisions.
{"title":"Using Gene Expression Profile to Extract the Biomarker Genes of Cardiovascular Disease","authors":"Hala M. Alshamlan","doi":"10.21786/bbrc/16.1.7","DOIUrl":"https://doi.org/10.21786/bbrc/16.1.7","url":null,"abstract":"Cardiovascular disease (CVD) is the world’s premier cause of morbidity and death. CVD is a class of heart or blood vessel diseases. CVD contains the coronary artery disease (CAD) such as unstable angina (UA) and myocardial infarction (MI) diseases. Clinicians use additional tools to support clinical evaluation and improve their ability to detect the susceptible patient at threat for CVD. Biomarkers are one such method to identify potential risk persons, rapidly and reliably diagnose disease symptoms that efficiently predict and treat disease. Discovery of MicroRNAs (miRNAs) representing a class of small, non-coding RNA molecules opens interesting opportunities to use the patterns of miRNAs as a biomarker for cardiovascular diseases. The objective of this study is to define miRNA and genes potentially associated with MI. Rothman dataset includes 52 samples of Acute Coronary Syndromes (ACS). including 18 patients with myocardial infarction (MI) and 8 patients with unstable angina (UA). Overall (number of genes selected) candidate ncRNA biomarkers have been defined and a ncRNA-based classifier has been created to predict MI risk which based on 7 ncRNA expression data using vector support machines SVM and decision tree classifiers. The experimental results obtained through applying these mechanisms on the Rothman dataset. The classification model’s performance is evaluated using the V-fold validation and LOOCV methods. The outcome of this search can be used by the drug designer for pathway analysis and CVD treatment decisions.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46468190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Most of the murders are shrouded in mystery and the absence of a lot of forensic evidence that helps the authorities achieve justice and reveal the culprit. The insects present at the crime scene and their various stages remain clues and evidence that are very important in knowing and revealing the circumstances of the incident. Therefore, determining the species of insect related to forensic medicine is very important for each region separately. The present study was planned to survey of forensic insects species and its seasonal activity in Makkah governorate. The local white rabbits were used as a mammal closer to the human corpse. The work was carried out of one year from January 2007 to December 2019. During the survey, 15,757 flies of importance forensic insects were collected which represent three families: Muscidae (47.1%)،Calliphoridae (30.0%); Sarcophagidae (22.6%), and Muscidae (47.1%). In this study, four flies species were reported for the first time in Makkah City in the Kingdom of Saudi Arabia, these were Chrysomya albiceps; Ch. marginalis; Sarcophaga dux and Wohlfahrtia bella. The appearance of the W. bella species was first recorded in Saudi Arabia. The results showed the presence of forensic insects most of the time during the year, and there were significant differences in different months of the year, and the reason for this may be due to differences in climatic conditions, temperature, and relative humidity which are considered among the most important factors affecting the reproduction and spread of the insects.
{"title":"16.3.3First Survey and Identification of Forensically Important Entomofauna in Makkah City","authors":"Habeeb M Al-Solami","doi":"10.21786/bbrc/16.1.3","DOIUrl":"https://doi.org/10.21786/bbrc/16.1.3","url":null,"abstract":"Most of the murders are shrouded in mystery and the absence of a lot of forensic evidence that helps the authorities achieve justice and reveal the culprit. The insects present at the crime scene and their various stages remain clues and evidence that are very important in knowing and revealing the circumstances of the incident. Therefore, determining the species of insect related to forensic medicine is very important for each region separately. The present study was planned to survey of forensic insects species and its seasonal activity in Makkah governorate. The local white rabbits were used as a mammal closer to the human corpse. The work was carried out of one year from January 2007 to December 2019. During the survey, 15,757 flies of importance forensic insects were collected which represent three families: Muscidae (47.1%)،Calliphoridae (30.0%); Sarcophagidae (22.6%), and Muscidae (47.1%). In this study, four flies species were reported for the first time in Makkah City in the Kingdom of Saudi Arabia, these were Chrysomya albiceps; Ch. marginalis; Sarcophaga dux and Wohlfahrtia bella. The appearance of the W. bella species was first recorded in Saudi Arabia. The results showed the presence of forensic insects most of the time during the year, and there were significant differences in different months of the year, and the reason for this may be due to differences in climatic conditions, temperature, and relative humidity which are considered among the most important factors affecting the reproduction and spread of the insects.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":"235 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136046642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cellobiose dehydrogenase (CDH) enzyme is secreted extracellularly by wood-rotting fungi of the phyla Basidiomycetes and Ascomycetes. The reducing ends of cellobiose, lactose, and maltose are oxidized by CDH to produce their respective lactones. These lactones are consequently converted into their carboxylic acids such as cellobionic acid, lactobionic acid, and maltobionic acid. Due to its commercial unavailability and its applications in various fields, there is a need for cost-effective CDH production. In the present work, Schizophyllum commune BCC26414 has been used for CDH production by solid-state fermentation (SSF). CDH production was optimized by one factor at a time (OFAT) approach in terms of initial moisture content, inoculum size, incubation temperature, particle size, and fermentation time. BBD (Box-Behnken Design) was used to perform statistical optimization of CDH production using statistical software, Response Surface Methodology (RSM) Maximum CDH production was obtained when groundnut shell was used as a substrate at 30°C on 9th day of incubation, with 0.5mm to 1mm particle size, 2 ml inoculum size, and the initial moisture content 50% using Schizophyllum commune BCC26414. RSM enhances enzyme production to 1.6-fold as compared to unoptimized conditions. This is the first report on solid-state CDH production using groundnut shells as solid substrate. A variety of CDH applications have been reported in the fields of biomedical, biocatalysts, bioremediation, and biosensors. This study will be helpful in the cost-effective production of CDH for various applications.
{"title":"Solid State Fermentation of Groundnut Shell by Schizophyllum commune BCC26414 for Production of Cellobiose Dehydrogenase","authors":"Vinita Yadav","doi":"10.21786/bbrc/16.1.6","DOIUrl":"https://doi.org/10.21786/bbrc/16.1.6","url":null,"abstract":"Cellobiose dehydrogenase (CDH) enzyme is secreted extracellularly by wood-rotting fungi of the phyla Basidiomycetes and Ascomycetes. The reducing ends of cellobiose, lactose, and maltose are oxidized by CDH to produce their respective lactones. These lactones are consequently converted into their carboxylic acids such as cellobionic acid, lactobionic acid, and maltobionic acid. Due to its commercial unavailability and its applications in various fields, there is a need for cost-effective CDH production. In the present work, Schizophyllum commune BCC26414 has been used for CDH production by solid-state fermentation (SSF). CDH production was optimized by one factor at a time (OFAT) approach in terms of initial moisture content, inoculum size, incubation temperature, particle size, and fermentation time. BBD (Box-Behnken Design) was used to perform statistical optimization of CDH production using statistical software, Response Surface Methodology (RSM) Maximum CDH production was obtained when groundnut shell was used as a substrate at 30°C on 9th day of incubation, with 0.5mm to 1mm particle size, 2 ml inoculum size, and the initial moisture content 50% using Schizophyllum commune BCC26414. RSM enhances enzyme production to 1.6-fold as compared to unoptimized conditions. This is the first report on solid-state CDH production using groundnut shells as solid substrate. A variety of CDH applications have been reported in the fields of biomedical, biocatalysts, bioremediation, and biosensors. This study will be helpful in the cost-effective production of CDH for various applications.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45394141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Currently, new technological advances in biomedicine make the creation of multidisciplinary teams of vital importance. These groups can be made up clinicians, epidemiologists, mathematicians, statisticians, computer scientists, biologists, among others, all together they can achieve an accurate prediction of infectious diseases and thus draw up the appropriate strategies by the competent authorities. The fundamental objective of this work is to obtain, through Regressive Objective Regession (ROR), the modeling of the next positive case that arrived with COVID-19 without performing PCR at the “Marta Abreu” Trashing Polyclinic in the city of Santa Clara. In this work, daily data were used from January to March corresponding to the year 2021 of the number of Covid-19 cases in the “Marta Abreu” Teaching Polyclinic in the city of Santa Clara, in the province of Villa Clara, Cuba, a total of 3294 cases of them 58 positive, of which they are assigned in the database an order number (No) according to how they were registered in the database. In the short-term modeling, the model was assigned to 19.7% with an error of0.12 the dichotomous variables, saw tooth and inverted saw tooth, and the risk returned in 1.3 and 12 cases, the trend is negative and not significant. The ROR modeling of predictions obtained give very significant results for the study of the COVID-19 pandemic at the Marta Abreu Teaching Polyclinic. With the results of the study, the authorities are provided, and in fact they are already doing so, with information on the short-and medium-term behavior of variables of great interest to understand the expansion of SARS-CoV2, which could be used for decision-marking.
{"title":"Modeling of a Positive Case of Covid-19 Through Regressive Objective Regression Without Doing PCR","authors":"Melba Zayas González","doi":"10.21786/bbrc/16.2.2","DOIUrl":"https://doi.org/10.21786/bbrc/16.2.2","url":null,"abstract":"Currently, new technological advances in biomedicine make the creation of multidisciplinary teams of vital importance. These groups can be made up clinicians, epidemiologists, mathematicians, statisticians, computer scientists, biologists, among others, all together they can achieve an accurate prediction of infectious diseases and thus draw up the appropriate strategies by the competent authorities. The fundamental objective of this work is to obtain, through Regressive Objective Regession (ROR), the modeling of the next positive case that arrived with COVID-19 without performing PCR at the “Marta Abreu” Trashing Polyclinic in the city of Santa Clara. In this work, daily data were used from January to March corresponding to the year 2021 of the number of Covid-19 cases in the “Marta Abreu” Teaching Polyclinic in the city of Santa Clara, in the province of Villa Clara, Cuba, a total of 3294 cases of them 58 positive, of which they are assigned in the database an order number (No) according to how they were registered in the database. In the short-term modeling, the model was assigned to 19.7% with an error of0.12 the dichotomous variables, saw tooth and inverted saw tooth, and the risk returned in 1.3 and 12 cases, the trend is negative and not significant. The ROR modeling of predictions obtained give very significant results for the study of the COVID-19 pandemic at the Marta Abreu Teaching Polyclinic. With the results of the study, the authorities are provided, and in fact they are already doing so, with information on the short-and medium-term behavior of variables of great interest to understand the expansion of SARS-CoV2, which could be used for decision-marking.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46747950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Processing of sensory input entails recognizing, categorizing, and interpreting data to generate an adaptive reaction. The sensory sub-types include barognosis, stereognosis, graphesthesia, two-point discrimination, and tactile localization. Stereognosis is the capacity to recognise the form and shape of a three-dimensional item and, consequently, its identity, by tactile manipulation of that object in the absence of visual and aural inputs. Therefore, manual stereognosis is necessary for integrating all hand sensations. So, the aim of this study is to determine the normal reaction time of manual stereognosis in adults.A pilot cross-sectional study was done on age group of 18-40 years. The stereognosis test was performed first on dominant hand and then on non-dominant hand using 5 objects (key, coin, safety pin, comb, pencil) with participant’s eyes closed during the test. The time taken to identify the objects was recorded using stopwatch. Then, mean normal reaction time was calculated. Student t-test was used to compare any differences between right & left-hand side stereognosis and between male & female gender.Results from the study show normal mean values of reaction time in seconds was 3.04 for right hand and 2.09 for left hand in adults. Data also shows that there were statistically significant differences between right & left-hand side stereognosis. However, no differences were found between male & female stereognosis using independent t-test.These normative results of data will serve as a useful, simple, reproducible, rapid assessment of stereognosis and also aid in planning of treatment.
{"title":"Normative Reference Value for Stereognosis Among Adult Population: A Cross-Sectional Pilot Study","authors":"Malika Malika","doi":"10.21786/bbrc/16.1.4","DOIUrl":"https://doi.org/10.21786/bbrc/16.1.4","url":null,"abstract":"Processing of sensory input entails recognizing, categorizing, and interpreting data to generate an adaptive reaction. The sensory sub-types include barognosis, stereognosis, graphesthesia, two-point discrimination, and tactile localization. Stereognosis is the capacity to recognise the form and shape of a three-dimensional item and, consequently, its identity, by tactile manipulation of that object in the absence of visual and aural inputs. Therefore, manual stereognosis is necessary for integrating all hand sensations. So, the aim of this study is to determine the normal reaction time of manual stereognosis in adults.A pilot cross-sectional study was done on age group of 18-40 years. The stereognosis test was performed first on dominant hand and then on non-dominant hand using 5 objects (key, coin, safety pin, comb, pencil) with participant’s eyes closed during the test. The time taken to identify the objects was recorded using stopwatch. Then, mean normal reaction time was calculated. Student t-test was used to compare any differences between right & left-hand side stereognosis and between male & female gender.Results from the study show normal mean values of reaction time in seconds was 3.04 for right hand and 2.09 for left hand in adults. Data also shows that there were statistically significant differences between right & left-hand side stereognosis. However, no differences were found between male & female stereognosis using independent t-test.These normative results of data will serve as a useful, simple, reproducible, rapid assessment of stereognosis and also aid in planning of treatment.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":"93 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68353367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Most of the murders are shrouded in mystery and the absence of a lot of forensic evidence that helps the authorities achieve justice and reveal the culprit. The insects present at the crime scene and their various stages remain clues and evidence that are very important in knowing and revealing the circumstances of the incident. Therefore, determining the species of insect related to forensic medicine is very important for each region separately. The present study was planned to survey of forensic insects species and its seasonal activity in Makkah governorate. The local white rabbits were used as a mammal closer to the human corpse. The work was carried out of one year from January 2007 to December 2019. During the survey, 15,757 flies of importance forensic insects were collected which represent three families: Muscidae (47.1%) ،Calliphoridae (30.0%); Sarcophagidae (22.6%), and Muscidae (47.1%). In this study, four flies species were reported for the first time in Makkah City in the Kingdom of Saudi Arabia, these were Chrysomya albiceps; Ch. marginalis; Sarcophaga dux and Wohlfahrtia bella. The appearance of the W. bella species was first recorded in Saudi Arabia. The results showed the presence of forensic insects most of the time during the year, and there were significant differences in different months of the year, and the reason for this may be due to differences in climatic conditions, temperature, and relative humidity which are considered among the most important factors affecting the reproduction and spread of the insects.
{"title":"First Survey and Identification of Forensically Important Entomofauna in Makkah City","authors":"H. M. Al-solami","doi":"10.21786/bbrc/16.3.3","DOIUrl":"https://doi.org/10.21786/bbrc/16.3.3","url":null,"abstract":"Most of the murders are shrouded in mystery and the absence of a lot of forensic evidence that helps the authorities achieve justice and reveal the culprit. The insects present at the crime scene and their various stages remain clues and evidence that are very important in knowing and revealing the circumstances of the incident. Therefore, determining the species of insect related to forensic medicine is very important for each region separately. The present study was planned to survey of forensic insects species and its seasonal activity in Makkah governorate. The local white rabbits were used as a mammal closer to the human corpse. The work was carried out of one year from January 2007 to December 2019. During the survey, 15,757 flies of importance forensic insects were collected which represent three families: Muscidae (47.1%) ،Calliphoridae (30.0%); Sarcophagidae (22.6%), and Muscidae (47.1%). In this study, four flies species were reported for the first time in Makkah City in the Kingdom of Saudi Arabia, these were Chrysomya albiceps; Ch. marginalis; Sarcophaga dux and Wohlfahrtia bella. The appearance of the W. bella species was first recorded in Saudi Arabia. The results showed the presence of forensic insects most of the time during the year, and there were significant differences in different months of the year, and the reason for this may be due to differences in climatic conditions, temperature, and relative humidity which are considered among the most important factors affecting the reproduction and spread of the insects.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47648201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pain neuroscience education ( PNE) is a new concept which helps the patients to modulate their pain perception and experiences. The concept has shown effectiveness, comparable to other pain-relieving modalities including manual therapies.The objective of the current review is to explore the mechanism on which pain neurosciences work and various methodologies to implement pain neuroscience education. The current literature of pain neurosciences education has ben reviewed and the evidences synthesised to explore the above objectives. Pain neuroscience education takes into account the complexity of a pain experience and aids patients in redefining pain by educating them on the various neurophysiological, neurobiological, social, and physical factors that may be present in their particular pain experience. The main objective of PNE is to assist individuals in rethinking their chronic pain. The mechanism through which PNE works is reframing the way we think about pain. It is important to accept that in chronic pain, pain is not a direct measure of tissue damage and more chronic the pain it becomes less reliable gauge for tissue damage. It is also important to note that pain is influences by physical, psychological and social factors According to research, pain neuroscience education is a successful therapy technique for a number of chronic pain subtypes, including whiplash-related pain, neck pain in teenagers, and pain from lumbar surgery, complicated regional pain syndrome, and low back pain. PNE has advantages for participants from early adolescence to old persons. Pain Neuroscience Education is an effective method of managing pain and may be used by the physiotherapist to manage the patients with musculoskeletal conditions Chronic musculoskeletal pain is very common complaint and research shows that pain neuroscience education is effective in pain management and physiotherapists should utilize the concept in patients as it improves patient engagement in health care.
{"title":"Evidence of Pain Neuroscience Education on Musculoskeletal Disorders: An Updated Review","authors":"S. Sunidhi","doi":"10.21786/bbrc/16.1.1","DOIUrl":"https://doi.org/10.21786/bbrc/16.1.1","url":null,"abstract":"Pain neuroscience education ( PNE) is a new concept which helps the patients to modulate their pain perception and experiences. The concept has shown effectiveness, comparable to other pain-relieving modalities including manual therapies.The objective of the current review is to explore the mechanism on which pain neurosciences work and various methodologies to implement pain neuroscience education. The current literature of pain neurosciences education has ben reviewed and the evidences synthesised to explore the above objectives. Pain neuroscience education takes into account the complexity of a pain experience and aids patients in redefining pain by educating them on the various neurophysiological, neurobiological, social, and physical factors that may be present in their particular pain experience. The main objective of PNE is to assist individuals in rethinking their chronic pain. The mechanism through which PNE works is reframing the way we think about pain. It is important to accept that in chronic pain, pain is not a direct measure of tissue damage and more chronic the pain it becomes less reliable gauge for tissue damage. It is also important to note that pain is influences by physical, psychological and social factors According to research, pain neuroscience education is a successful therapy technique for a number of chronic pain subtypes, including whiplash-related pain, neck pain in teenagers, and pain from lumbar surgery, complicated regional pain syndrome, and low back pain. PNE has advantages for participants from early adolescence to old persons. Pain Neuroscience Education is an effective method of managing pain and may be used by the physiotherapist to manage the patients with musculoskeletal conditions Chronic musculoskeletal pain is very common complaint and research shows that pain neuroscience education is effective in pain management and physiotherapists should utilize the concept in patients as it improves patient engagement in health care.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43087893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Polygonum is an important genus of Polygonaceae and Polygonum posumbu is underutilized traditional medicinal plant whose leaves are extensively used as an antipyretic and dyspepsia agent in Manipur, India as well as a spice in India, Japan, Nepal, China, South Korea, Philippines and Thailand. P. posumbu is commonly known as Phak-pai in Manipur. DNA barcode based molecular characterization technology for species identification has been recognized as a reliable tool for plants but the selection of suitable universal marker is still under discussion. The main aim of the study is to identify the Polygonum posumbu sample from Manipur, India using DNA barcode method. In the present study, Polygonum posumbu samples were collected from a local farmer in Awang khunou, Imphal West, Manipur, India which was taxonomically identified. To assess the molecular species identification, Internal Transcribed spacer (ITS) marker system was employed and the primer’s pair used were covered the internal transcribed spacer 1, 5.8S ribosomal RNA gene and internal transcribed spacer 2 complete sequence. Phylogenetic tree was constructed for the studied sample with the sequences of the 9 species retrieved from the GenBank in order to show the relationships among the samples. The result shows that the ITS marker-based DNA identification method successfully characterized the Polygonum posumbu species. The information of ITS marker-based identification of Polygonum posumbu from Manipur, India, will enhance our knowledge in better understanding the medicinal properties of this plant.
{"title":"Molecular Identification of Medicinal Plant, Polygonum posumbu from Manipur, India","authors":"Premi Devi Pukhrambam","doi":"10.21786/bbrc/16.1.8","DOIUrl":"https://doi.org/10.21786/bbrc/16.1.8","url":null,"abstract":"Polygonum is an important genus of Polygonaceae and Polygonum posumbu is underutilized traditional medicinal plant whose leaves are extensively used as an antipyretic and dyspepsia agent in Manipur, India as well as a spice in India, Japan, Nepal, China, South Korea, Philippines and Thailand. P. posumbu is commonly known as Phak-pai in Manipur. DNA barcode based molecular characterization technology for species identification has been recognized as a reliable tool for plants but the selection of suitable universal marker is still under discussion. The main aim of the study is to identify the Polygonum posumbu sample from Manipur, India using DNA barcode method. In the present study, Polygonum posumbu samples were collected from a local farmer in Awang khunou, Imphal West, Manipur, India which was taxonomically identified. To assess the molecular species identification, Internal Transcribed spacer (ITS) marker system was employed and the primer’s pair used were covered the internal transcribed spacer 1, 5.8S ribosomal RNA gene and internal transcribed spacer 2 complete sequence. Phylogenetic tree was constructed for the studied sample with the sequences of the 9 species retrieved from the GenBank in order to show the relationships among the samples. The result shows that the ITS marker-based DNA identification method successfully characterized the Polygonum posumbu species. The information of ITS marker-based identification of Polygonum posumbu from Manipur, India, will enhance our knowledge in better understanding the medicinal properties of this plant.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45324011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Currently, new technological advances in biomedicine make the creation of multidisciplinary teams of vital importance. These groups can be made up clinicians, epidemiologists, mathematicians, statisticians, computer scientists, biologists, among others, all together they can achieve an accurate prediction of infectious diseases and thus draw up the appropriate strategies by the competent authorities. The fundamental objective of this work is to obtain, through Regressive Objective Regession (ROR), the modeling of the next positive case that arrived with COVID-19 without performing PCR at the “Marta Abreu” Trashing Polyclinic in the city of Santa Clara. In this work, daily data were used from January to March corresponding to the year 2021 of the number of Covid-19 cases in the “Marta Abreu” Teaching Polyclinic in the city of Santa Clara, in the province of Villa Clara, Cuba, a total of 3294 cases of them 58 positive, of which they are assigned in the database an order number (No) according to how they were registered in the database. In the short-term modeling, the model was assigned to 19.7% with an error of0.12 the dichotomous variables, saw tooth and inverted saw tooth, and the risk returned in 1.3 and 12 cases, the trend is negative and not significant. The ROR modeling of predictions obtained give very significant results for the study of the COVID-19 pandemic at the Marta Abreu Teaching Polyclinic. With the results of the study, the authorities are provided, and in fact they are already doing so, with information on the short-and medium-term behavior of variables of great interest to understand the expansion of SARS-CoV2, which could be used for decision-marking.
{"title":"Modeling of a Positive Case of Covid-19 Through Regressive Objective Regression Without Doing PCR","authors":"Melba Zayas González","doi":"10.21786/bbrc/16.1.2","DOIUrl":"https://doi.org/10.21786/bbrc/16.1.2","url":null,"abstract":"Currently, new technological advances in biomedicine make the creation of multidisciplinary teams of vital importance. These groups can be made up clinicians, epidemiologists, mathematicians, statisticians, computer scientists, biologists, among others, all together they can achieve an accurate prediction of infectious diseases and thus draw up the appropriate strategies by the competent authorities. The fundamental objective of this work is to obtain, through Regressive Objective Regession (ROR), the modeling of the next positive case that arrived with COVID-19 without performing PCR at the “Marta Abreu” Trashing Polyclinic in the city of Santa Clara. In this work, daily data were used from January to March corresponding to the year 2021 of the number of Covid-19 cases in the “Marta Abreu” Teaching Polyclinic in the city of Santa Clara, in the province of Villa Clara, Cuba, a total of 3294 cases of them 58 positive, of which they are assigned in the database an order number (No) according to how they were registered in the database. In the short-term modeling, the model was assigned to 19.7% with an error of0.12 the dichotomous variables, saw tooth and inverted saw tooth, and the risk returned in 1.3 and 12 cases, the trend is negative and not significant. The ROR modeling of predictions obtained give very significant results for the study of the COVID-19 pandemic at the Marta Abreu Teaching Polyclinic. With the results of the study, the authorities are provided, and in fact they are already doing so, with information on the short-and medium-term behavior of variables of great interest to understand the expansion of SARS-CoV2, which could be used for decision-marking.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":"25 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136046643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
With an increasing rate of global warming and unstable climatic conditions concerns with regards to epidemiology of plant viruses are on the rise. Studies suggest accelerating climatic changes shall severely affect the management of pest and diseases in cultivated crops. Tomato Leaf Curl Virus (TLCV) is an economically affecting viral infections of tomato (Lycopersicum esculentum). The disease causes severe yield loss and major economic impairment. The current study was therefore taken up to understand the influence of agro climatic zones on diversity of TLCV in Tomato plants. Samples of TLCV infected Tomato plants exhibiting varying symptoms were collected from seven different agro climatic zones of Gujarat followed by isolation of viral particles, molecular characterization and development of phylogenetic tree. Interestingly the molecular analysis of the isolated viral samples indicated little influence of climatic conditions on the types of TLCV infecting the tomato plants.
{"title":"Phylogeny of Strains of Tomato Leaf Curl Virus from Agroclimatic Zones of Gujarat","authors":"Patel Golden T","doi":"10.21786/bbrc/16.1.5","DOIUrl":"https://doi.org/10.21786/bbrc/16.1.5","url":null,"abstract":"With an increasing rate of global warming and unstable climatic conditions concerns with regards to epidemiology of plant viruses are on the rise. Studies suggest accelerating climatic changes shall severely affect the management of pest and diseases in cultivated crops. Tomato Leaf Curl Virus (TLCV) is an economically affecting viral infections of tomato (Lycopersicum esculentum). The disease causes severe yield loss and major economic impairment. The current study was therefore taken up to understand the influence of agro climatic zones on diversity of TLCV in Tomato plants. Samples of TLCV infected Tomato plants exhibiting varying symptoms were collected from seven different agro climatic zones of Gujarat followed by isolation of viral particles, molecular characterization and development of phylogenetic tree. Interestingly the molecular analysis of the isolated viral samples indicated little influence of climatic conditions on the types of TLCV infecting the tomato plants.","PeriodicalId":9156,"journal":{"name":"Bioscience Biotechnology Research Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49266925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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