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IOT Based Paraplegia Patient Communication Device Using Smart Glove 使用智能手套的基于物联网的截瘫患者通信设备
Pub Date : 2023-03-25 DOI: 10.21786/bbrc/16.1.11
B. S
Paraplegia is a paralysis that affects the legs and often seriously affects the lower half of a human body. Paraplegia happens whilst there may be damage underneath the neck. The maximum common motive is trauma, such as sports injury or vehicle accident. other causes are stroke, spine tumors, together with cancers. This paper provides a novel design to enhance the conversation of paralysis sufferers. The device is a clever glove that is used to seize hand gestures and convert them into verbal commands. The glove is geared up with sensors and processors that discover the hand gestures and convert them into indicators. The tool is designed to be simple and user-friendly, allowing patients to quickly adapt to its use. The tool is likewise designed to be low-cost and handy, permitting more paralysis patients to use it. The device is tested and evaluated for its performance, and the outcomes display that it may accurately capture and interpret the hand gestures of the sufferers. In most cases paralyzed affected person (like Monoplegia, Hemiplegia, Paraplegia) aren’t capable of interact or communicate with others or their caretaker. This net of factors primarily based clever glove facilitates the affected person to convey their needs to the caretakers. It measures pulse, temperature, blood pressure and gyro. In gyroscope sensor there is a message, based at the route or motion in which it can be conveyed thru show and audio. it is able to screen thru internet and smart telephones. but it can not be used for tetraplegia patient. It is concluded that in quadriplegia as patients can not move their body, if in future we can able to read the neuron signals of the patients through sensor or any device and then we can able to understand what they need or what they want. Consequently the system can be able to display or hear through audio which can be used according to the need, updating into neuro reading sensors which are economically viable.
截瘫是一种影响腿部的瘫痪,通常严重影响人体的下半身。截瘫发生时,颈部下方可能有损伤。最常见的动机是创伤,如运动损伤或交通事故。其他原因包括中风、脊柱肿瘤以及癌症。本文提供了一种新颖的设计来增强瘫痪患者的对话。该设备是一种智能手套,用于捕捉手势并将其转换为口头命令。这款手套配备了传感器和处理器,可以发现手势并将其转换为指示器。该工具设计简单,用户友好,允许患者快速适应其使用。该工具同样被设计为低成本和方便,允许更多的瘫痪患者使用它。对该设备的性能进行了测试和评估,结果表明它可以准确地捕捉和解释患者的手势。在大多数情况下,瘫痪的人(如单侧瘫痪、偏瘫、截瘫)不能与他人或他们的看护人互动或交流。这张主要基于智能手套的因素网有助于受影响的人向照顾者传达他们的需求。它可以测量脉搏、体温、血压和陀螺仪。在陀螺仪传感器有一个信息,基于路线或运动,它可以通过显示和音频传达。它可以通过互联网和智能手机进行筛查。但不能用于四肢瘫痪患者。结论是,四肢瘫痪的病人不能移动他们的身体,如果将来我们能够通过传感器或任何设备读取病人的神经元信号,然后我们就能理解他们需要什么或想要什么。因此,该系统可以根据需要显示或听到可使用的音频,更新为经济可行的神经读取传感器。
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引用次数: 0
Bimetallic Nanoparticles – Expanding Grassroots in Medical Health Care through Enhanced Microbial Resistance 双金属纳米颗粒-通过增强微生物耐药性扩大基层医疗保健
Pub Date : 2023-03-25 DOI: 10.21786/bbrc/16.1.10
Laxmi Bhatti
Nanotechnology has spawned a slew of new research and application opportunities. Bimetallic nanoparticles offer certain advantages over monometallic nanoparticles in magnetic and optical properties which have utmost requirement in medicinal industry for therapeutics and drug delivery system. The advantage of using a green synthesis technique results in increased stability and enhancement of many physical and chemical characteristics. Green synthesis has the benefit of being able to replace existing physical and chemical processes. Different monometallic nanoparticles have already been tested. Researchers are working on even trimetallic nanoparticles in present scenario. In the present investigation research cum review article, we have randomly selected medicinal plants available in campus itself as lockdown conditions were prevalent all over the country.The bimetallic Cu-Zn nanoparticles were synthesized using mostly leaves as plant parts, from these selected plant materials.The Cu-Zn nanoparticles were confirmed by their synthesis, using visual color change along with UV visible spectroscopy techniques. The nanoparticles after confirmation were opted further for testing their efficacy against standard pathogens for plants viz Terminalia, Tecoma and Solanum nigrum.The results have been promising and significant giving zones bigger in size than 20 mm.The research is ongoing to reveal further the inherent potential of medicinal plants to act as alternative agents for antimicrobial potential.
纳米技术产生了大量新的研究和应用机会。双金属纳米粒子在磁性和光学性能方面比单金属纳米粒子具有一定的优势,在医药行业的治疗和给药系统中有着极大的需求。使用绿色合成技术的优点是增加了稳定性并增强了许多物理和化学特性。绿色合成的好处是能够取代现有的物理和化学过程。不同的单金属纳米颗粒已经进行了测试。在目前的情况下,研究人员正在研究三金属纳米颗粒。在本次调查研究和综述文章中,我们随机选择了校园内可用的药用植物,因为全国各地都有封锁条件。以这些选定的植物材料为原料,主要以植物叶片为原料合成了双金属铜锌纳米颗粒。利用视觉颜色变化和紫外可见光谱技术合成了Cu-Zn纳米颗粒。确认后的纳米颗粒被进一步选择用于测试它们对植物如Terminalia、Tecoma和Solanum nigrum的标准病原体的功效。研究结果显示,这些区域的面积大于20毫米。研究还在继续,以进一步揭示药用植物作为抗菌潜力替代剂的内在潜力。
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引用次数: 0
Effect of Multi-Functional Therapeutic Active Extract of Marine Asterias rubens against Tooth Decaying Pathogens 海苔多功能治疗活性提取物对抗蛀牙病原体的作用
Pub Date : 2022-12-25 DOI: 10.21786/bbrc/15.4.5
V. Vijayalakshmi
Recently, childhood caries have become one of the main health related diseases , especially developed by lactic acid bacteria, affecting millions of school going children worldwide. In the present work, we have isolated about 50 lactic acid bacteria samples from different tooth decay affected children from Fen Dental Clinic, Tirupur India. The emerging bacteria were confirmed by phenotype and genotype characterization. After, the pathogens were screened for the production of biofilm it was checked for the resistant mechanism of the Lactobacillus acidophilus. In this study the MTLA1-50 isolates produced strong biofilms which were confirmed for the Lactobacillus acidophilus carrying resistant capability. In particularly, the maximum strong biofilm producing strains were MTLA 32 and MTLA 50, followed by other isolates. As star fish extract has been reported to play a vital role in eliminating the bacterial biofilm for this motive in this study we have chosen the star fish, Asterias rubens extract in the concentration of 50, 100, 150 µl. The analysis was performed and executed using well diffusion standard techniques. The results showed that the strain No. MTLA 32 and MTLA 50 got the maximum zone of inhibition at 150 µl (52mm and 38 mm) of the star fish extract. The findings demonstrate that the star fish extract has a powerful anti-biotic ability to treat the dental pathogens particularly, caused by Lactobacillus acidophilus.
近年来,儿童龋齿已成为主要的健康相关疾病之一,尤其是由乳酸菌引起的,影响着全球数百万在校儿童。在目前的工作中,我们从印度蒂鲁普尔芬牙科诊所的不同蛀牙影响儿童中分离出了大约50个乳酸菌样本。通过表型和基因型鉴定证实了新出现的细菌。然后,对产生生物膜的病原体进行筛选,并检查嗜酸乳杆菌的抗性机制。在本研究中,MTLA1-50分离株产生了强大的生物膜,证实嗜酸乳杆菌具有抗性。特别是,最大的强生物膜产生菌株是MTLA 32和MTLA 50,其次是其他分离株。据报道,由于星鱼提取物在消除细菌生物膜方面发挥着至关重要的作用,因此在本研究中,我们选择了浓度为50、100、150µl的星鱼——红Asterias rubens提取物。使用井扩散标准技术进行和执行分析。结果表明,菌株MTLA 32和MTLA 50在150µl(52mm和38mm)的星鱼提取物处获得最大抑制区。研究结果表明,星鱼提取物具有强大的抗菌能力,可以治疗牙齿病原体,尤其是由嗜酸乳杆菌引起的病原体。
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引用次数: 0
16s rRNA Amplicon Sequencing Approach for Community and Predictive Functional Diversity of Therapeutically Valuable Formulation of Cow-derivatives 16s rRNA扩增子测序方法用于具有治疗价值的奶牛衍生物配方的群落和预测功能多样性
Pub Date : 2022-12-25 DOI: 10.21786/bbrc/15.4.10
Nilam Vaghamshi
Panchagavya is the blend of five ingredients obtained from cows and used in traditional Hindu rituals. Panchagavya preparation is a microbial mediated process that possibly involves microbial succession. The present study was conducted to decode the microbial community that exists in the preparation of three hours of old Panchagavya. DNA was isolated from Panchagavya using HiPurA™ Stool DNA Purification Kit followed by quality checking by Agarose electrophoresis and Qubit fluorometer. The V3 and V4 region of 16s rRNA based amplicon sequencing was performed using the Illumine MiSeq platform. Taxonomic profile encoded by using One Codex, kraken and MG-RAST. Functional traits detected through the abundance of specific genes using Tax4Fun. Taxonomic result suggests the total 2000 species were identified. The most abundant was Streptomyces griseocarneus (2.65%) followed by Clostridiales bacterium (2.26%), Bacteroidales bacterium (1.38%), and Verrucomicrobia bacterium (1.13%). Community based analysis revealed the microbial diversity and presence of anaerobic, unclassified, and uncultivable microbes in metagenomes, which may be associated with the pharmacological properties of Panchagavya. Functional analysis predicts around 351 metabolic pathways for metabolism of carbohydrates, synthesis of secondary metabolites and degradation of xenobiotic compounds. The detection of various secondary metabolites genes associated with pharmacological molecules correlated with its traditional clinical applications. The present study revealed the advantages of cultivation approach for exploring untapped and unique bacterial diversity, and also utilities for various biotechnological and environmental applications.
Panchagavya是从牛身上提取的五种成分的混合物,用于传统的印度教仪式。Panchagavya制备是一个微生物介导的过程,可能涉及微生物演替。本研究是为了解码存在于三小时旧潘查加维亚制备中的微生物群落。使用HiPurA™粪便DNA纯化试剂盒从Panchagavya中分离DNA,然后通过琼脂糖电泳和量子比特荧光仪进行质量检测。采用Illumine MiSeq平台对16s rRNA扩增子的V3和V4区进行测序。使用One Codex、kraken和MG-RAST编码的分类资料。利用Tax4Fun通过特异基因丰度检测功能性状。分类结果表明,共鉴定出2000种。丰度最高的是灰褐色链霉菌(2.65%),其次是梭菌(2.26%)、拟杆菌(1.38%)和Verrucomicrobia细菌(1.13%)。基于群落的分析显示,宏基因组中存在厌氧、未分类和不可培养的微生物,这可能与Panchagavya的药理特性有关。功能分析预测了大约351种代谢途径,包括碳水化合物的代谢、次级代谢物的合成和外源化合物的降解。与药理分子相关的各种次生代谢物基因的检测与其传统的临床应用相关。本研究揭示了培养方法在探索尚未开发和独特的细菌多样性方面的优势,以及在各种生物技术和环境应用方面的实用性。
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引用次数: 0
Incidence of Climate on the Number of Admissions and Deaths Due to Cerebrovascular Diseases Through Mathematical Modeling In Sagua La Grande, Villa Clara, Cuba 通过数学模型研究古巴Villa Clara Sagua La Grande地区气候对脑血管疾病入院和死亡人数的影响
Pub Date : 2022-12-25 DOI: 10.21786/bbrc/15.4.11
Jorge Luis Alonso Freyre
The number of deceased cases and admissions for annual cerebrovascular diseases were modeled in Sagua la Grande, Villa Clara province, Cuba, between 1993 and 2017. A statistical description of these two variables was made, with an average admission of 148 .7 cases, with a standard deviation of 24.9. The deceased had an average of 35.7 with a standard deviation of 13.2, the results were obtained using the Regressive Objective Regression (ROR) methodology. The impact of the minimum temperature and atmospheric pressure of the Sagua La Grande station was studied, reaching the conclusion that as the minimum temperature increases, the income and deaths increase, so this impact is related to the climate change, when the minimum temperature increases by 1 °C, deaths increase by 9 cases, while when the maximum temperature increases by 1 °C, deaths increase by 11.6 cases. In the case of revenue, as the atmospheric pressure increases by 2 hPa, the amount of revenue decreases by 1 case.
1993年至2017年期间,在古巴比利亚克拉拉省的萨瓜拉格兰德,模拟了死亡病例和年度脑血管疾病入院人数。对这两个变量进行统计描述,平均入院148.7例,标准差为24.9。死者的平均年龄为35.7,标准差为13.2,结果采用回归客观回归(ROR)方法获得。对Sagua La Grande站的最低气温和气压的影响进行了研究,得出随着最低气温的升高,收入和死亡人数增加,因此这种影响与气候变化有关,最低气温每升高1℃,死亡人数增加9例,最高气温每升高1℃,死亡人数增加11.6例。在收入的情况下,大气压力每增加2 hPa,收入的数量减少1例。
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引用次数: 0
Bacteriological and Physicochemical Evaluation of Selected Bottled Water Brands in Nasarawa, Kano State, Nigeria 尼日利亚卡诺州纳萨拉瓦选定瓶装水品牌的细菌学和理化评价
Pub Date : 2022-12-25 DOI: 10.21786/bbrc/15.4.4
M. Abdulsalam
Twenty-six different brands of bottled water were tested for bacteriological and physicochemical qualities in Nasarawa Local Government Area of Kano State, Nigeria. Bacteriological assessments have been used to outline the wide varieties of heterotrophic bacteria, overall coliform and faecal coliform. The chloride content, pH, temperature, conductivity, flouride content, total hardness and suspended particles have been additionally investigated. The coliform count ranged from 0 to 1000 coliform/100ml of the sample while the heterotrophic bacterial count ranged from 0 to 118 cfu/ml. In the bottled water samples, there was no faecal coliform and fluoride contents. The pH, temperature, conductivity, Chloride content, total hardness, and total suspended particles were all within acceptable limits between 6.50 and 8.30, 19.8 and 21.00C, 63.10 and 86.92µS/cm, 3.58 and 4.64 mg/l, 1 and 86 mg/l and 1.0 x 10-3 to 3.0 x 10-3 g/100ml, respectively. Staphylococcus, Aeromonas, Klebsiella, Micrococcus and Bacillus were among the bacterial species found. For heterotrophic bacteria, total coliform and faecal coliform, 25 (96%), 24 (92%) and 0 (100%) of the 26 different bottled water samples met the standard respectively. For pH, temperature, conductivity, fluoride content, chloride content, total hardness and total suspended particles, all of the bottled water samples also met the criteria. According to the results of this study, 88.46 percent of the bottled waters tested were fit for consumption based on bacteriological and physicochemical factors. When producing bottled water, it is recommended that stringent criteria should be followed to ensure that the bottles are well capped, and that statistics including the producing date, expiration date and consignment number be imprinted on the bottles.
在尼日利亚卡诺州纳萨拉瓦地方政府区,对26种不同品牌的瓶装水进行了细菌和物理化学性质测试。细菌学评估已被用于概述各种各样的异养细菌、总大肠菌群和粪便大肠菌群。此外,还对氯化物含量、pH、温度、电导率、氟化物含量、总硬度和悬浮颗粒进行了研究。大肠菌群计数范围为0-1000大肠菌群/100毫升,而异养细菌计数范围为0-118 cfu/ml。在瓶装水样本中,没有粪便大肠菌群和氟化物含量。pH、温度、电导率、氯化物含量、总硬度和总悬浮颗粒均在可接受范围内,分别为6.50和8.30、19.8和21.00C、63.10和86.92µS/cm、3.58和4.64 mg/l、1和86 mg/l以及1.0 x 10-3至3.0 x 10-3 g/100ml。发现的细菌种类包括葡萄球菌、气单胞菌、克雷伯菌、微球菌和芽孢杆菌。在异养细菌、总大肠菌群和粪大肠菌群方面,26个不同瓶装水样本中分别有25个(96%)、24个(92%)和0个(100%)符合标准。在pH、温度、电导率、氟化物含量、氯化物含量、总硬度和总悬浮颗粒方面,所有瓶装水样品也符合标准。根据这项研究的结果,根据细菌和物理化学因素,88.46%的瓶装水适合饮用。在生产瓶装水时,建议遵循严格的标准,以确保瓶子盖好,并在瓶子上印上包括生产日期、有效期和发货编号在内的统计数据。
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引用次数: 0
Water Resources of Rivers and Erosion-Accumulation Processes 河流水资源与侵蚀堆积过程
Pub Date : 2022-12-25 DOI: 10.21786/bbrc/15.4.1
F. Lisetskii
Basin territorial structures are defined as unified natural and anthropogenic systems, since the movement of matter, energy and information occurs naturally from the divide to the valley line of the river valley within them. Considering the global issues of soil degradation in watersheds and depletion of water resources, some regional experience in the design and implementation of soil and water protection of cultivated lands as part of the concept of the basin nature management and implementation of the program for environmental rehabilitation of rivers and water bodies is presented. The statement that it is necessary to develop a long-term strategy for the consistent arrangement of basin geosystems from a divide of catchments to valley lines of a fluvial network in the conditions of a crisis situation with the use of soil, land and water resources is justified.
流域地域结构被定义为统一的自然系统和人为系统,因为物质、能量和信息在其中自然地从分水岭向河谷线运动。考虑到流域土壤退化和水资源枯竭的全球性问题,介绍了作为流域自然管理概念和实施河流和水体环境恢复方案的一部分,在设计和实施耕地水土保护方面的一些区域经验。有必要制定一项长期战略,以便在使用土壤、土地和水资源的危机情况下,从集水区到河流网的河谷线的流域地质系统的一致安排,这种说法是合理的。
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引用次数: 1
Quality Enhancement of Multifocus & Medical Images Using Hybrid Wavelets Based Fusion for Bioengineering Applications 生物工程应用中基于混合小波的融合增强多焦点医学图像的质量
Pub Date : 2022-12-25 DOI: 10.21786/bbrc/15.4.6
C. Mohan
Multifocus image fusion employs fusion principles to integrate many focused images of the same scene. All-in-focus images are instructive and valuable for visual perception. Maintaining shift-invariant and directional selectivity in a fused image is crucial. Traditional wavelet-based fusion methods are hindered their performance due to a lack of invariant shift and reduced directionality. In this paper, a classical multifocus hybrid wavelet-based approach with principal component analysis (PCA) is proposed. At the first level of decomposition, stationary wavelet transformation (SWT) is used to perform the fusion process with the given source images. In the next level, approximation coefficients of source images are selected for decomposition as well as fusion using dual-tree complex wavelet transformation (DTCWT) and finally, PCA is applied to generate a final fused image. Analysis of the proposed method has been accomplished by evaluating various objective parameters.
多焦点图像融合采用融合原理对同一场景的多个聚焦图像进行融合。全聚焦图像对视觉感知具有指导意义和价值。在融合图像中保持移位不变和方向选择性是至关重要的。传统的基于小波的融合方法由于缺乏不变移位和方向性降低而阻碍了其性能。本文提出了一种经典的基于多焦点混合小波的主成分分析方法。在第一级分解中,使用平稳小波变换(SWT)对给定的源图像进行融合处理。在下一级中,使用双树复小波变换(DTCWT)选择源图像的近似系数进行分解和融合,最后应用PCA生成最终的融合图像。通过评估各种目标参数,对所提出的方法进行了分析。
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引用次数: 0
Acute Toxicity Alterations in Oxidative Stress Enzymes and Biochemical Parameters in Oreochromis mossambicus, Induced by Cartap Hydrochloride and the Modulatory Effects of Ocimum sanctum Supplementation 盐酸Cartap对莫桑比克罗非鱼氧化应激酶和生化指标的急性毒性变化及补药对其的调节作用
Pub Date : 2022-12-25 DOI: 10.21786/bbrc/15.4.8
Sharmistha Medda
The present study was assessed to determine the acute toxicity and the changes in oxidative stress enzymes and some other biochemical parameters at the sublethal level of a thiocarbamate pesticide cartap hydrochloride on freshwater fish Oreochromis mossambicus. The study reveals that the 96h median lethal concentration (LC50) value of cartap hydrochloride is 20.7 µg/l. Besides, the exposed fish also exhibited erratic behavioral responses at the acute level. The effects of cartap hydrochloride at the sublethal concentration (30% of 96h LC50 value) after 15d and 30d exposure induces alterations in biochemical parameters of freshwater fish Oreochromis mossambicus. Moreover, the modulatory effects of Ocimum sanctum powder (20 gm/kg feed) on the toxicity of cartap hydrochloride were investigated. The investigation demonstrated that sublethal concentrations of cartap hydrochloride increased the levels of liver catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), malondialdehyde (MDA), aspartate aminotransferase (AST), and alanine aminotransferase (ALT). Additionally, the exposed fish treated with dietary Ocimum sanctum abridged the toxic effects of the pesticide. Moreover by using integrated biomarker response (IBR) and biomarker response index (BRI) the change in the health status of pesticide exposed fish upon addition of Ocimum sanctum supplemented diet over control diet was determined. These results indicate that cartap hydrochloride alters the survivability and behavioral responses of Oreochromis mossambicus at the acute level and changes the biochemical parameters at the sublethal level which was modulated by the additament of Ocimum sanctum.
本研究旨在测定硫代氨基甲酸酯类农药盐酸卡他普对淡水鱼的急性毒性以及在亚致死水平下氧化应激酶和其他一些生化参数的变化。研究表明,盐酸卡丹96小时中位致死浓度(LC50)值为20.7µg/l。此外,暴露的鱼类在急性水平上也表现出不稳定的行为反应。暴露15d和30d后,亚致死浓度(96h LC50值的30%)的盐酸卡他普的作用引起淡水鱼Oreochromis mossambicus生化参数的改变。此外,还研究了香蒲粉(20克/公斤饲料)对盐酸卡丹毒性的调节作用。研究表明,亚致死浓度的盐酸卡丹可提高肝脏过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽S-转移酶(GST)、丙二醛(MDA)、天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)的水平。此外,暴露在外的鱼类用饲料中的Ocimum sanctum处理,减少了杀虫剂的毒性作用。此外,通过使用综合生物标志物反应(IBR)和生物标志物响应指数(BRI),测定了添加Ocimum sancum补充日粮后暴露于农药的鱼类健康状况的变化。这些结果表明,盐酸卡他普在急性水平上改变了Oreochromis mossambicus的生存能力和行为反应,并在亚致死水平上改变由Ocimum santum添加剂调节的生化参数。
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引用次数: 0
Effect of Phytohormones on In-vitro Bud and Root Formation of the Water Hyacinth, Eichhornia crassipes 植物激素对水葫芦离体芽和根形成的影响
Pub Date : 2022-12-25 DOI: 10.21786/bbrc/15.4.3
Thi Anh Thu Tran
Water hyacinth is an aquatic plant, of which proliferation rate is extremely rapid as a weed, causing great economic, social and environmental damage. On the other hand, the water hyacinth also has potential economic value, because it is used as food for livestock, gas, fertilizer, environmental treatment, art crafts, decoration items, as well as herbal medicine. This research investigated the effects of concentrations of BA (Benzyl adenine) and NAA (Naphthylacetic acid) on in-vitro bud and root formation of Eichhornia crassipes [Mart.] Solms to create in-vitro sample source which is initially used in subsequent researches of water hyacinth. After 4 weeks of in-vitro culture, the results showed that the two-layer MS (Murashige Skoog) medium – the lower solid agar medium and the upper liquid medium – accompanied by aerobic culture conditions supplemented with 0.5 mg/L BA were suitable for bud proliferation. Next, these explants after destroying shoot apical meristem were transferred to MS medium supplemented with 0.75 mg/L BA suitable for bud development. The mature buds were transferred to MS medium supplemented with 0.25 mg/L NAA, suitable for rooting of water hyacinth and gave high survival rate (83.00%) when planted in the garden on hydroponic nutrient medium Howard of 600ppm.
风信子是一种水生植物,其作为杂草的增殖速度极快,对经济、社会和环境造成了巨大的破坏。另一方面,水葫芦也具有潜在的经济价值,因为它被用作牲畜的食物、天然气、肥料、环境处理、工艺美术、装饰物品以及草药。本研究研究了BA(苄基腺嘌呤)和NAA(萘乙酸)浓度对凤眼莲(Eichhornia crassipes[Mart.]Solms)离体芽和根形成的影响,建立了初步用于后续凤眼莲研究的离体样品来源。体外培养4周后,结果表明,双层MS(Murashige Skoog)培养基——下部固体琼脂培养基和上部液体培养基——配以添加0.5mg/L BA的好氧培养条件适合芽增殖。然后,将这些破坏了茎尖分生组织的外植体转移到添加了0.75mg/L BA的MS培养基中,该培养基适合芽的发育。将成熟芽转移到附加0.25mg/L NAA的MS培养基中,该培养基适合水葫芦生根,在600ppm的霍华德水培营养培养基上种植,成活率高(83.00%)。
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引用次数: 0
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Bioscience Biotechnology Research Communications
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