Seven smut fungi belonging to the genus Microbotryum are reported for the first time from the following Balkan countries: M. heliospermatis, M. piperi, M. scabiosae, M. silenes-dioicae, and M. silenes-saxifragae from Bulgaria, M. reticulatum, M. silenes-saxifragae, and M. stygium from Greece, and M. silenes-saxifragae from Bosnia and Herzegovina and Croatia. The finding of M. piperi represents the first Balkan record of this smut fungus which, elsewhere in Europe, is only known from the French Pyrenees and the Italian Alps. The finding of M. stygium in Crete considerably extends its distribution in Europe. Rumex tuberosus subsp. creticus is reported as a new host for M. stygium.
{"title":"New records of Microbotryum (Microbotryaceae) from the Balkan Peninsula","authors":"T. Denchev, B. Assyov, C. Denchev","doi":"10.2298/botserb2102285d","DOIUrl":"https://doi.org/10.2298/botserb2102285d","url":null,"abstract":"Seven smut fungi belonging to the genus Microbotryum are reported for the first time from the following Balkan countries: M. heliospermatis, M. piperi, M. scabiosae, M. silenes-dioicae, and M. silenes-saxifragae from Bulgaria, M. reticulatum, M. silenes-saxifragae, and M. stygium from Greece, and M. silenes-saxifragae from Bosnia and Herzegovina and Croatia. The finding of M. piperi represents the first Balkan record of this smut fungus which, elsewhere in Europe, is only known from the French Pyrenees and the Italian Alps. The finding of M. stygium in Crete considerably extends its distribution in Europe. Rumex tuberosus subsp. creticus is reported as a new host for M. stygium.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"7 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74563137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Sabovljević, G. Tomović, P. Lazarević, V. Stojanović, Simona Strgulc-Krajsek, D. Kosič, L. Kutnar, Janez Kermavnar, Petya Boycheva, G. Yaneva, Dobri Ivanov, S. Vukojičić, Sanja Djurovic, Uroš Buzurović, M. Niketić, B. Assyov, S. Ștefănuț
This paper presents new records and noteworthy data on the following taxa in SE Europe and adjacent regions: saprotrophic fungi Psathyrella typhae, Stropharia halophila, mosses Bryum klingraeffii and Buxbaumia viridis, liverwort Scapania gymnostomophila, fern Matteuccia struthiopteris, monocots Galanthus nivalis, Listera ovata, and Ophrys apifera, and dicots Astragalus dasyanthus and Paeonia officinalis subsp. banatica.
{"title":"New records and noteworthy data of plants, algae and fungi in SE Europe and adjacent regions, 5","authors":"M. Sabovljević, G. Tomović, P. Lazarević, V. Stojanović, Simona Strgulc-Krajsek, D. Kosič, L. Kutnar, Janez Kermavnar, Petya Boycheva, G. Yaneva, Dobri Ivanov, S. Vukojičić, Sanja Djurovic, Uroš Buzurović, M. Niketić, B. Assyov, S. Ștefănuț","doi":"10.2298/botserb2102353s","DOIUrl":"https://doi.org/10.2298/botserb2102353s","url":null,"abstract":"This paper presents new records and noteworthy data on the following taxa in SE Europe and adjacent regions: saprotrophic fungi Psathyrella typhae, Stropharia halophila, mosses Bryum klingraeffii and Buxbaumia viridis, liverwort Scapania gymnostomophila, fern Matteuccia struthiopteris, monocots Galanthus nivalis, Listera ovata, and Ophrys apifera, and dicots Astragalus dasyanthus and Paeonia officinalis subsp. banatica.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"44 2 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89548083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Selected locations in four Balkan countries (Serbia, Albania, North Macedonia and Greece) were visited. One hundred and twenty one lichen species were recorded in fourteen locations. Cladonia cervicornis is reported new to Serbia, as well as Cladonia squamosa, Pertusaria leioplaca, Xanthoparmelia angustiphylla and Polycauliona polycarpa to Albania. One lichenicolous fungus Plectocarpon lichenum was recorded. A brief description of the new or interesting records of these species is also provided.
{"title":"Noteworthy lichens recorded in the Balkan Peninsula","authors":"M. Marcinčinová, M. Goga, H. Mayrhofer, M. Bačkor","doi":"10.2298/botserb2102303m","DOIUrl":"https://doi.org/10.2298/botserb2102303m","url":null,"abstract":"Selected locations in four Balkan countries (Serbia, Albania, North Macedonia and Greece) were visited. One hundred and twenty one lichen species were recorded in fourteen locations. Cladonia cervicornis is reported new to Serbia, as well as Cladonia squamosa, Pertusaria leioplaca, Xanthoparmelia angustiphylla and Polycauliona polycarpa to Albania. One lichenicolous fungus Plectocarpon lichenum was recorded. A brief description of the new or interesting records of these species is also provided.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"38 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82895004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The first observation of the red alga Bangia atropurpurea (Bangiales, Rhodophyta) in Serbia was in 1991 in the Trgoviski Timok River (Eastern Serbia). From 1991 to 2017, the species was sporadically observed at four more locations in three hillmountain rivers in Southwestern (the Gvozdacka River and the Raska River) and Eastern (the Nisava River - two localities) Serbia. More intensive research on this alga was carried out from 2017 to 2020, and a total of 220 localities in 132 hillmountain watercourses were studied. Bangia atropurpurea was recorded at six locations in Southwestern and Eastern Serbia. Five of these locations represent new B. atropurpurea localities in Serbia (the Brusnicka River, the Golijska Moravica River, the Panjica River, the Resava River, and the second locality in the Trgoviski Timok River), while one of them is already known from previous studies (the Raska River). The species was found in running, moderately hard and very hard, well-oxygenated, slightly alkaline waters, mainly low in inorganic nutrients, and in highly variable values of temperature and conductivity. Despite the long period since its first observation in Serbian rivers, the species is still known in a small number of localities. The small number of localities, low relative abundance, and various anthropogenic influences affecting its habitats have led the species to be recognized as endangered and strictly protected in Serbia. The intensive development of small hydropower plants which have emerged in recent years may threaten B. atropurpurea habitats.
{"title":"On Bangia atropurpurea (Bangiales, Rhodophyta), the strictly protected red alga in Serbia","authors":"Aleksandra Mitrović, S. Simić","doi":"10.2298/botserb2102263m","DOIUrl":"https://doi.org/10.2298/botserb2102263m","url":null,"abstract":"The first observation of the red alga Bangia atropurpurea (Bangiales, Rhodophyta) in Serbia was in 1991 in the Trgoviski Timok River (Eastern Serbia). From 1991 to 2017, the species was sporadically observed at four more locations in three hillmountain rivers in Southwestern (the Gvozdacka River and the Raska River) and Eastern (the Nisava River - two localities) Serbia. More intensive research on this alga was carried out from 2017 to 2020, and a total of 220 localities in 132 hillmountain watercourses were studied. Bangia atropurpurea was recorded at six locations in Southwestern and Eastern Serbia. Five of these locations represent new B. atropurpurea localities in Serbia (the Brusnicka River, the Golijska Moravica River, the Panjica River, the Resava River, and the second locality in the Trgoviski Timok River), while one of them is already known from previous studies (the Raska River). The species was found in running, moderately hard and very hard, well-oxygenated, slightly alkaline waters, mainly low in inorganic nutrients, and in highly variable values of temperature and conductivity. Despite the long period since its first observation in Serbian rivers, the species is still known in a small number of localities. The small number of localities, low relative abundance, and various anthropogenic influences affecting its habitats have led the species to be recognized as endangered and strictly protected in Serbia. The intensive development of small hydropower plants which have emerged in recent years may threaten B. atropurpurea habitats.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"2 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78830981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Djukic, S. Marković, J. Mastilović, Predrag Simović
Proline is one of the organic osmolytes that accumulates in plants in response to stressful environmental conditions. The aim of this study was to determine the concentration of proline in the grain filling phase in ten winter wheat cultivars under conditions of prolonged periods of air temperatures above 30?C and to assess the variability of wheat variety responses. The correlation between proline accumulation and photosynthetic pigments, accumulated starch and yield was also determined. In the experimental field, flag leaves were sampled for each variety in the grain filling phase. Statistical data analysis and parameter correlation were performed using the SPSS program. The results showed that there is a statistically significant difference between proline values in moderate and high air temperature conditions when proline accumulation occurs. The significant correlation of accumulated starch content and yield with proline concentration showed that in addition to higher proline values under heat stress, wheat varieties Apac, Talas and Futura also have higher yield and starch content. A correlation was also found between proline concentration and photosynthetic pigment contents, where the Apac wheat variety showed the best adaptive response to the investigated traits and was characterized by a smaller reduction in photosynthetic pigment content under heat stress. The interrelation of proline with the photosynthetic pigment content and wheat quality parameters may be important in breeding technologies aimed at improving wheat stress tolerance.
{"title":"Differences in proline accumulation between wheat varieties in response to heat stress","authors":"N. Djukic, S. Marković, J. Mastilović, Predrag Simović","doi":"10.2298/BOTSERB2101061D","DOIUrl":"https://doi.org/10.2298/BOTSERB2101061D","url":null,"abstract":"Proline is one of the organic osmolytes that accumulates in plants in response to stressful environmental conditions. The aim of this study was to determine the concentration of proline in the grain filling phase in ten winter wheat cultivars under conditions of prolonged periods of air temperatures above 30?C and to assess the variability of wheat variety responses. The correlation between proline accumulation and photosynthetic pigments, accumulated starch and yield was also determined. In the experimental field, flag leaves were sampled for each variety in the grain filling phase. Statistical data analysis and parameter correlation were performed using the SPSS program. The results showed that there is a statistically significant difference between proline values in moderate and high air temperature conditions when proline accumulation occurs. The significant correlation of accumulated starch content and yield with proline concentration showed that in addition to higher proline values under heat stress, wheat varieties Apac, Talas and Futura also have higher yield and starch content. A correlation was also found between proline concentration and photosynthetic pigment contents, where the Apac wheat variety showed the best adaptive response to the investigated traits and was characterized by a smaller reduction in photosynthetic pigment content under heat stress. The interrelation of proline with the photosynthetic pigment content and wheat quality parameters may be important in breeding technologies aimed at improving wheat stress tolerance.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"70 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90660144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Radojević, M. Grujović, L. Čomić, M. Dekić, G. Djelic, N. Mihailovic
Achillea ageratifolia subsp. serbica is a poorly investigated endemic of the Balkan area and there are almost no data about the potential application of this plant. The purpose of this study was the investigation of the phytochemical composition, antioxidant, antimicrobial, and antibiofilm activity of its water, acetone, and ethyl acetate extracts. The phenolic composition and flavonoids were established using high-performance liquid chromatography with photodiode array detection (HPLC-PDA) analysis, while the chemical composition of the ethyl acetate extracts of the aerial parts of the plant was investigated by gas chromatography-mass spectrometry (GC and GC-MS). Antioxidant and antimicrobial activity, as well as the inhibition ability of biofilm formation on Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853 biofilms, were also investigated. The total phenolic content ranged from 18.61 mgGAE/g to 43.78 mgGAE/g of extract. The total flavonoid content ranged from 10.79 mgRUE/g to 34.02 mgRUE/g. The acetone and ethyl acetate extracts of the aerial parts contained chlorogenic acid, myricetin, apigenin, and luteolin. Analysis of the volatile specialized metabolites in the ethyl acetate extract of the aerial parts allowed for the identification of thirty-two constituents. The highest antioxidant activity was detected in the water extract of the aerial parts of the plant (IC50 = 641.06 ?g/ml) and in the ethyl acetate extract of the root (IC50 = 675.33 ?g/ml). Bacillus subtilis and S. aureus showed higher sensitivity to the tested extracts, while the antifungal activity of the marked extracts was significant. All extracts showed a moderate inhibitory effect on the ability of biofilm formation of P. aeruginosa ATCC 27853, while only the ethyl acetate inflorescence extract showed an effect on the biofilm formation of P. aeruginosa ATCC 27853. The tested extracts showed the potential for further investigation and possible application as biofungicides.
{"title":"The phytochemical composition and biological activities of different types of extracts of Achillea ageratifolia subsp. serbica","authors":"I. Radojević, M. Grujović, L. Čomić, M. Dekić, G. Djelic, N. Mihailovic","doi":"10.2298/BOTSERB2101049R","DOIUrl":"https://doi.org/10.2298/BOTSERB2101049R","url":null,"abstract":"Achillea ageratifolia subsp. serbica is a poorly investigated endemic of the Balkan area and there are almost no data about the potential application of this plant. The purpose of this study was the investigation of the phytochemical composition, antioxidant, antimicrobial, and antibiofilm activity of its water, acetone, and ethyl acetate extracts. The phenolic composition and flavonoids were established using high-performance liquid chromatography with photodiode array detection (HPLC-PDA) analysis, while the chemical composition of the ethyl acetate extracts of the aerial parts of the plant was investigated by gas chromatography-mass spectrometry (GC and GC-MS). Antioxidant and antimicrobial activity, as well as the inhibition ability of biofilm formation on Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853 biofilms, were also investigated. The total phenolic content ranged from 18.61 mgGAE/g to 43.78 mgGAE/g of extract. The total flavonoid content ranged from 10.79 mgRUE/g to 34.02 mgRUE/g. The acetone and ethyl acetate extracts of the aerial parts contained chlorogenic acid, myricetin, apigenin, and luteolin. Analysis of the volatile specialized metabolites in the ethyl acetate extract of the aerial parts allowed for the identification of thirty-two constituents. The highest antioxidant activity was detected in the water extract of the aerial parts of the plant (IC50 = 641.06 ?g/ml) and in the ethyl acetate extract of the root (IC50 = 675.33 ?g/ml). Bacillus subtilis and S. aureus showed higher sensitivity to the tested extracts, while the antifungal activity of the marked extracts was significant. All extracts showed a moderate inhibitory effect on the ability of biofilm formation of P. aeruginosa ATCC 27853, while only the ethyl acetate inflorescence extract showed an effect on the biofilm formation of P. aeruginosa ATCC 27853. The tested extracts showed the potential for further investigation and possible application as biofungicides.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"14 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87186503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Assyov, F. Bozok, M. Yarar, M. Slavova, H. Taşkın
The authors present a contribution to Amanita alseides, so far known from its type specimens from France and Italy. A detailed description is included, based on molecularly supported collections from Bulgaria and Greece, expanding the knowledge on the morphological variability and the distribution of the species.
{"title":"A contribution to Amanita alseides, a recently described European species in the section vaginatae","authors":"B. Assyov, F. Bozok, M. Yarar, M. Slavova, H. Taşkın","doi":"10.2298/BOTSERB2101143A","DOIUrl":"https://doi.org/10.2298/BOTSERB2101143A","url":null,"abstract":"The authors present a contribution to Amanita alseides, so far known from its type specimens from France and Italy. A detailed description is included, based on molecularly supported collections from Bulgaria and Greece, expanding the knowledge on the morphological variability and the distribution of the species.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"115 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81663170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bojan Veljković, Ivana T. Karabegović, S. Aćić, M. Topuzović, I. Petrović, S. Savic, Z. Dajić-Stevanović
An ethnobotanical survey of the traditional use of the wild raspberry (Rubus ideaus) was conducted on nine sites in the regions of southwest (SWS) and southeast (SES) Serbia. The regions studied belong to mountainous rural areas in Serbia, mainly populated by Serbian people of the Orthodox faith. In total, 93 respondents were interviewed about the medicinal, food and cosmetic uses of the wild raspberry. In terms of plant parts in traditional use, the leaf and fruit are known to be used frequently. The dry leaf is more commonly used in the SES (78%) than in the SWS (25%) region. In the SWS region, the most frequent use of the wild raspberry leaf is linked to gastrointestinal disorders and prostate inflammation. In the SES region, the leaves are mostly used for the prevention and healing of gynecological disorders during both pregnancy and lactation. The local population of the SWS region uses the fruits mainly for the preparation of syrup juice (92%), fruit preserves (42%) and in desserts (62%), while in the SES area the fruits are most frequently used for the preparation of nonalcoholic (97.4%) and alcoholic beverages (35.9%). Our results provide some novel information on the use of the wild raspberry in Serbia and in the Balkans, such as for the treatment of prostate inflammation, use during breastfeeding and to alleviate inflammation of the eyes.
{"title":"The wild raspberry in Serbia: An ethnobotanical study","authors":"Bojan Veljković, Ivana T. Karabegović, S. Aćić, M. Topuzović, I. Petrović, S. Savic, Z. Dajić-Stevanović","doi":"10.2298/BOTSERB2101107V","DOIUrl":"https://doi.org/10.2298/BOTSERB2101107V","url":null,"abstract":"An ethnobotanical survey of the traditional use of the wild raspberry (Rubus ideaus) was conducted on nine sites in the regions of southwest (SWS) and southeast (SES) Serbia. The regions studied belong to mountainous rural areas in Serbia, mainly populated by Serbian people of the Orthodox faith. In total, 93 respondents were interviewed about the medicinal, food and cosmetic uses of the wild raspberry. In terms of plant parts in traditional use, the leaf and fruit are known to be used frequently. The dry leaf is more commonly used in the SES (78%) than in the SWS (25%) region. In the SWS region, the most frequent use of the wild raspberry leaf is linked to gastrointestinal disorders and prostate inflammation. In the SES region, the leaves are mostly used for the prevention and healing of gynecological disorders during both pregnancy and lactation. The local population of the SWS region uses the fruits mainly for the preparation of syrup juice (92%), fruit preserves (42%) and in desserts (62%), while in the SES area the fruits are most frequently used for the preparation of nonalcoholic (97.4%) and alcoholic beverages (35.9%). Our results provide some novel information on the use of the wild raspberry in Serbia and in the Balkans, such as for the treatment of prostate inflammation, use during breastfeeding and to alleviate inflammation of the eyes.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"151 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81145191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, arsenic (As)-induced genotoxicity in the roots and shoots of Cucumis melo (melon) seedlings were investigated by using the random amplified polymorphic DNA - polymerase chain reaction (RAPD-PCR) and comet assays. For this purpose, melon seedlings were exposed to arsenate [As(V)] at 0, 100, 200, 300, and 400 ?M concentrations in the hydroponic system for 14 days to examine the level of As accumulation, alterations in growth parameters, and DNA damage. A reduction in growth with increasing As(V) concentration was observed in the melon seedlings. Total As accumulations in the shoot and root tissue increased in a dose-dependent manner; however, the level was higher in the roots than the shoots. In RAPD-PCR analysis, 26 primers gave reproducible and scorable results and produced a total of 128 bands in the control seedlings. Alterations in RAPD profiles, including the loss or appearance of new bands, were determined in the As-treated seedlings when compared to the control. The values of genomic template stability (GTS) were decreased by increasing the concentration of the As(V) in both tissue types. DNA strand breaks were observed in all the tested As(V) concentrations in the alkaline comet assay; furthermore, the loss of DNA integrity was higher with 300 and 400 ?M As(V) treatments. The results clearly indicate that the combination of DNA-based molecular and cytogenetic techniques (e.g. the comet assay) may be proposed as a reliable evaluation of genotoxicity in plants after exposure to heavy metal pollution.
{"title":"Estimation of arsenic-induced genotoxicity in melon (Cucumis melo) by using RAPD-PCR and comet assays","authors":"Yonca Surgun-Acar","doi":"10.2298/BOTSERB2101097S","DOIUrl":"https://doi.org/10.2298/BOTSERB2101097S","url":null,"abstract":"In this study, arsenic (As)-induced genotoxicity in the roots and shoots of Cucumis melo (melon) seedlings were investigated by using the random amplified polymorphic DNA - polymerase chain reaction (RAPD-PCR) and comet assays. For this purpose, melon seedlings were exposed to arsenate [As(V)] at 0, 100, 200, 300, and 400 ?M concentrations in the hydroponic system for 14 days to examine the level of As accumulation, alterations in growth parameters, and DNA damage. A reduction in growth with increasing As(V) concentration was observed in the melon seedlings. Total As accumulations in the shoot and root tissue increased in a dose-dependent manner; however, the level was higher in the roots than the shoots. In RAPD-PCR analysis, 26 primers gave reproducible and scorable results and produced a total of 128 bands in the control seedlings. Alterations in RAPD profiles, including the loss or appearance of new bands, were determined in the As-treated seedlings when compared to the control. The values of genomic template stability (GTS) were decreased by increasing the concentration of the As(V) in both tissue types. DNA strand breaks were observed in all the tested As(V) concentrations in the alkaline comet assay; furthermore, the loss of DNA integrity was higher with 300 and 400 ?M As(V) treatments. The results clearly indicate that the combination of DNA-based molecular and cytogenetic techniques (e.g. the comet assay) may be proposed as a reliable evaluation of genotoxicity in plants after exposure to heavy metal pollution.","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"28 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72934557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A study of the names used for the hybrids described between Verbascum chaixii (subsp. chaixii, subsp. austriacum) and V. lanatum is presented. On the basis of the historical background of these names and the examination of specimens, the neotypification of Borbas? V. semilanatum (V. chaixii subsp. austriacum ? V. lanatum) is proposed on a specimen preserved at GB. A new nothospecies (V. tommasinianum Sutory) is proposed for the hybrid between V. chaixii subsp. chaixii and V. lanatum (holotype at BRNM, isotypes at WU and COI).
{"title":"Nomenclatural questions on the hybrids between Verbascum chaixii s.l. and V. lanatum","authors":"K. Sutorý","doi":"10.2298/botserb2102341s","DOIUrl":"https://doi.org/10.2298/botserb2102341s","url":null,"abstract":"A study of the names used for the hybrids described between Verbascum chaixii (subsp. chaixii, subsp. austriacum) and V. lanatum is presented. On the basis of the historical background of these names and the examination of specimens, the neotypification of Borbas? V. semilanatum (V. chaixii subsp. austriacum ? V. lanatum) is proposed on a specimen preserved at GB. A new nothospecies (V. tommasinianum Sutory) is proposed for the hybrid between V. chaixii subsp. chaixii and V. lanatum (holotype at BRNM, isotypes at WU and COI).","PeriodicalId":9161,"journal":{"name":"Botanica Serbica","volume":"7 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82050152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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