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Maize landraces management to avoid transgenic contamination, decreases yield and seed quality 玉米地方品种管理避免转基因污染,降低产量和种子质量
IF 1.2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-06-21 DOI: 10.1590/1678-4499.20200421
Anderson Munarini, Daniele Nerling, C. Coelho, R. Nodari
ABSTRACT Producing high-quality seeds for agroecological agriculture is needed because the plants will be exposed to a diversity of environments and management practices. Sowing times and population densities can influence the quality of seeds produced by landraces. Further, seeds for agroecological cultivation must not be contaminated by transgenes. In this context, it is necessary to know the agronomic performance of landraces outside of the recommended growing period to avoid gene flow with transgenic cultivars. Thus, the objective of this work was to determine the germination rate, vigor and sanitary quality of seeds of maize genotypes cultivated in an agroecological system at different sowing times and densities. A split-split-plot design was used in the 2016/2017 and 2017/2018 growing seasons. The physiological quality evaluation was based on germination and vigor tests, and the sanitary quality was obtained by visually examining the seeds. The results showed a significant simple effect of genotype, sowing time and density, as well as double and triple interactions among factors for all evaluated traits. The most suitable time for sowing the corn landraces evaluated in an agroecological system in western Santa Catarina is September, when the seeds exhibited the highest percentage of germination and vigor and there was a lower incidence of pathogens. At this time, a density of 55,000 plants·ha–1 had the best result for germination. Late sowing was tested to avoid gene flow from transgenic varieties that usually are sown in September, but the results indicated penalties, such as a decrease in seed yield and quality.
为农业生态农业生产高质量种子是必要的,因为植物将暴露在多种环境和管理实践中。播种期和种群密度会影响地方品种的种子质量。此外,用于农业生态栽培的种子不得被转基因污染。在这种情况下,有必要了解地方品种在推荐生育期之外的农艺表现,以避免转基因品种的基因流动。因此,本研究的目的是确定在不同播种时间和密度的农业生态系统中栽培的玉米基因型种子的发芽率、活力和卫生质量。在2016/2017和2017/2018生长季节采用了分块-分块设计。生理品质评价以发芽和活力试验为依据,卫生品质评价以目测法为依据。结果表明,基因型、播期和密度对所有评价性状均有显著的单效应,各因子之间存在双交互作用和三交互作用。圣卡塔琳娜州西部农业生态系统评价的玉米地方品种最适宜播种时间为9月,种子发芽率和活力最高,病原菌发病率较低。此时,密度为55000株·ha-1时萌发效果最好。研究人员对晚播进行了试验,以避免通常在9月份播种的转基因品种的基因流动,但结果表明,晚播会造成种子产量和质量下降等后果。
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引用次数: 1
Growth and yield of sweet potato in response to the application of nitrogen rates and paclobutrazol 施氮量和多效唑对甘薯生长和产量的影响
IF 1.2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-06-21 DOI: 10.1590/1678-4499.20200447
N. P. Ribeiro, A. M. Fernandes, R. M. Silva, R. A. Pelvine, N. S. Assunção
ABSTRACT The excess of nitrogen (N) causes the overgrowth of sweet potato foliage, increasing the self-shading and reducing the root yield. Therefore, a combined N and paclobutrazol (PBZ) application can reduce the vegetative overgrowth and benefit the sweet potato yield. The purpose of this study was to evaluate the plant growth, yield, uptake and removal of N by sweet potato plants fertilized with N and treated with PBZ during two planting seasons. The treatments consisted of four N rates (0, 50, 100 and 200 kg·ha–1), three forms of PBZ application (PBZ-10: PBZ applied at 10 days after side-dressing N fertilization [DASNF]; PBZ-25: PBZ applied at 25 DASNF, and PBZ-10 + 25: PBZ applied at 10 plus 25 DASNF), and a control (without PBZ application). Paclobutrazol application temporarily reduced the length of the main branches of sweet potato planted in the rainy season, but it did not reduce the shoot biomass of plants in both planting seasons. A single application of PBZ at 10 DASNF increased the yield of fresh storage roots in the two planting seasons, but, during the rainy season, the increases were greater when two applications were carried out. The application of 50 kg·ha–1 N in the rainy season was sufficient to reach the maximum yield of fresh storage roots, but, in the dry season, the N fertilization increased the N uptake without benefiting the root yield.
氮素过量导致甘薯叶片过度生长,增加了自遮阳,降低了根系产量。因此,氮素与多效唑(PBZ)配施可以减少甘薯的营养过度生长,有利于产量的提高。本研究旨在评价施氮和PBZ处理对两个种植季甘薯植株生长、产量、氮吸收和氮去除的影响。4种施氮量(0、50、100和200 kg·ha-1), 3种不同施氮方式(PBZ-10:在侧施氮后10 d施PBZ [DASNF];PBZ-25:在25 DASNF下使用的PBZ, PBZ-10 + 25:在10 + 25 DASNF下使用的PBZ),和一个对照组(不使用PBZ)。施用多效唑可使雨季种植的甘薯主枝长度暂时缩短,但在两个种植季节均未减少植株的地上部生物量。在两个种植季节,以10 DASNF单次施用PBZ可增加鲜储根的产量,但在雨季,两次施用时增加的量更大。雨季施氮50 kg·hm - 1足以达到鲜储根的最大产量,但在旱季,施氮增加了氮的吸收,但对根系产量没有好处。
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引用次数: 1
New strains of Bradyrhizobium enrich plant biomass nitrogen content in Crotalaria for use as a green manure 利用慢生根瘤菌新菌株提高禾本科植物生物量氮含量用作绿肥
IF 1.2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-06-21 DOI: 10.1590/1678-4499.20200469
J. S. Silva, Dâmiany Pádua Oliveira, M. Rufini, Celso Leandro da Silva Junior, Márcia Baptista, Osnar Obede da Silva Aragão, Thiago de Assis Pereira, F. M. Moreira
ABSTRACT In spite of the chemical, physical, and biological benefits that Crotalaria spectabilis can provide to the soil, it is little used as a green manure crop by farmers. Inoculation with strains of legume-nodulating nitrogen-fixing bacteria that are efficient and competitive may be a strategy to enhance accumulation of N in C. spectabilis and stimulate adoption of this green manure crop. The aims of this study were i) to evaluate the symbiotic and agronomic efficiency of new strains of Bradyrhizobium on C. spectabilis in an oxisol (red latosol) compared to that of noninoculated controls (without and with mineral N) and with the approved strain BR2811, seeking to corroborate possible recommendation as inoculants for this species; and ii) to determine, the contribution of these treatments to N accumulation in the plant of C. spectabilis in four periods of cutting for determining possible and ideal periods for its incorporation in the soil. Experiments were carried out in pots and field. Inoculation with the new strains UFLA05-03, UFLA05-09, and UFLA05-14 and with BR2811 on C. spectabilis is effective since it increases the production of N-enriched plant biomass when compared to the control without N mineral. However, UFLA05-03 stands out among these strains because it behaves similarly to the control with mineral N both in relation to shoot N accumulation and dry matter just after 150 days in the field.
摘要:尽管赤豆能为土壤提供化学、物理和生物效益,但它很少被农民用作绿肥作物。接种高效且具有竞争力的豆类结瘤固氮菌菌株,可能是提高赤杨体内氮积累和促进绿肥作物采用的一种策略。本研究的目的是:i)与未接种对照(不含和含矿物质N)和已批准菌株BR2811相比,评估在oxisol(红赤霉菌)中的慢生根瘤菌新菌株对壮观梭菌的共生和农艺效率,试图证实作为该物种接种剂的可能推荐;和ii)确定这些处理在四个切割期内对壮观紫丁香植株中氮积累的贡献,以确定其融入土壤的可能和理想时期。实验在盆栽和田间进行。用新菌株UFLA05-03、UFLA05-09和UFLA05-14以及用BR2811在壮观芽孢杆菌上接种是有效的,因为与不含氮矿物的对照相比,它增加了富氮植物生物量的产量。然而,UFLA05-03在这些菌株中脱颖而出,因为它在田间150天后的芽氮积累和干物质方面的表现与矿物氮对照相似。
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引用次数: 0
Multi-trait genomic selection indexes applied to identification of superior genotypes 应用多性状基因组选择指标鉴定优良基因型
IF 1.2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-06-04 DOI: 10.1590/1678-4499.20200381
L. A. Silva, M. A. Peixoto, L. A. Peixoto, J. Romero, L. L. Bhering
ABSTRACT Most studies on genomic selection in plant breeding compare different statistical methods of univariate approach. However, multi-trait methodologies should be considered since they allow the simultaneous selection of superior genotypes in several economic traits. Here, the aims were to compare the selection accuracy and efficiency of the multivariate partial least square (MPLS) method compared with random regression best linear unbiased predictor (rrBLUP), Bayesian Lasso (Blasso) and univariate partial least square (UPLS) and to develop genomic selection indexes efficient for superior genotypes identification in plant breeding. Ten F2 populations with 800 individuals were simulated, considering four traits with different heritabilities. Genomic selection analyses using rrBLUP, Blasso, UPLS, and MPLS were conducted. Four genomic selection indexes were elaborated by the sum of the marker effects obtained for each trait, weighted by the respective residual variance. Multi-trait indexes were developed based on the assumptions of each methodology mentioned (rrBLUP, Blasso, UPLS, and MPLS), and were denominated I-rrBLUP, I-Blasso, I-UPLS, and I-MPLS. Processing time, selective accuracy, selection gains, and selection coincidence were used to compare the methods and the selection indexes proposed. The MPLS method had similar results compared to UPLS method for the low heritability traits and was less efficient than the rrBLUP and Blasso. The genome selection indexes provided the highest total genetic gains. The I-rrBLUP and I-MPLS indexes stood out for high efficiency in selecting superior genotypes in the shortest processing time. Results suggest that the genomic selection indexes proposed in this study may be promising for plant breeding programs.
大多数植物育种基因组选择的研究比较了单变量方法的不同统计方法。然而,应该考虑多性状方法,因为它们允许在几个经济性状中同时选择优越的基因型。本研究旨在比较多变量偏最小二乘(MPLS)方法与随机回归最佳线性无偏预测器(rrBLUP)、贝叶斯拉索(Blasso)和单变量偏最小二乘(UPLS)方法的选择精度和效率,并为植物育种中优质基因型的鉴定建立有效的基因组选择指标。考虑4种不同遗传力的性状,模拟10个F2群体,800个个体。使用rrBLUP、Blasso、UPLS和MPLS进行基因组选择分析。4个基因组选择指标由每个性状的标记效应之和和各自的残差方差加权来阐述。基于所提到的每种方法(rrBLUP、Blasso、UPLS和MPLS)的假设,开发了多性状指标,命名为I-rrBLUP、I-Blasso、I-UPLS和I-MPLS。利用处理时间、选择精度、选择增益和选择符合性对所提出的方法和选择指标进行了比较。对于低遗传力性状,MPLS方法与UPLS方法结果相似,但效率低于rrBLUP和Blasso。基因组选择指数提供了最高的总遗传增益。I-rrBLUP和I-MPLS指标在最短的处理时间内高效筛选出优良基因型。结果表明,本研究提出的基因组选择指标对植物育种具有一定的指导意义。
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引用次数: 2
Characterization and use efficiency of sparingly soluble fertilizer of boron and zinc for foliar application in coffee plants 咖啡树叶面疏溶硼锌肥特性及利用效率研究
IF 1.2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-06-04 DOI: 10.1590/1678-4499.20200329
L. O. Macedo, Dirceu Mattos Jr, Rodrigo Jacobassi, G. Petená, J. A. Quaggio, R. M. Boaretto
ABSTRACT Deficiencies of boron and zinc occur in coffee plantations despite the many fertilizer sources offered by the industry for the agriculture. Sparingly soluble fertilizers (SSF) have been used in several countries for many years. However, their effectiveness when applied to leaves is highly contested, and information about their use efficiency is still lacking. Experiments were set up to characterize the SSF of B and Zn according to particle size of fertilizers, and the spreading and retention of the particles in the leaf, comparing SSF (calcium borate, Zn oxide, and Zn borate) with soluble sources (boric acid and Zn sulfate) in four doses (mg.L-1): Experiment I (B 0, 130, 260, and 520), Experiment II (Zn 0, 200, 600, and 1,800), and experiment III (B 0, 43, 129, and 387, and Zn 0, 200, 600, and 1,800), to supply B and Zn for coffee plants. Microparticles of the SSF were found adhered to coffee leaves, and the Zn oxide was the one with the smallest particle size. SSFs were effective at increasing the Zn and B leaf concentrations in coffee. Dry matter of coffee increased 15% with applications of 270 mg.L-1 of B as calcium borate and 384 mg.L-1 of B as boric acid. Foliar application of Zn oxide at the dose of 1,800 mg.L-1 of Zn increased the leaf areas of the plants. High doses of Zn sulfate caused toxicity to coffee plants due to high saline concentrations. The microparticles found retained on the surface of the coffee leaves increased leaf concentration of B and Zn, which explained variations in the dry matter measurements and show the potential of the SSF for the development of fertilizers to improve the availability of micronutrients to coffee.
尽管咖啡工业为农业提供了许多肥料来源,但咖啡种植园仍然存在硼和锌的缺乏。疏溶性肥料(SSF)已在一些国家使用多年。然而,它们在叶片上的有效性存在很大争议,关于它们的使用效率的信息仍然缺乏。实验设置描述B的社保基金根据粒子大小和锌的肥料,和粒子的扩散和保留叶子,比较社保基金(氧化硼酸钙、锌和硼酸锌)和可溶性来源(硼酸和硫酸锌)在四个剂量(mg.L-1):实验我(B 0, 130, 260, 520),实验二世(锌0、200、600和1800年),和实验3 (B 0, 129, 387,和锌0,200年,600年和1800年),咖啡树提供B和锌。结果表明,咖啡叶表面吸附有SSF微粒,其中氧化锌的粒径最小。SSFs能有效提高咖啡叶片中锌和B的浓度。270毫克咖啡的干物质增加了15%。L-1的B作为硼酸钙384毫克。L-1的B变成硼酸。叶面施用氧化锌1800毫克。L-1 Zn可使植株叶面积增加。高剂量的硫酸锌由于高盐浓度而对咖啡植株产生毒性。残留在咖啡叶表面的微颗粒增加了叶片中B和Zn的浓度,这解释了干物质测量的变化,并显示了SSF在开发肥料方面的潜力,以提高咖啡中微量营养素的可用性。
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引用次数: 4
Yield related key traits in the selection of super sweetcorn hybrids 超级甜玉米杂交种选育中与产量相关的关键性状
IF 1.2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-06-04 DOI: 10.1590/1678-4499.20200484
W. L. Zaluski, M. V. Faria, J. Rosa, R. Uhdre, V. S. Sagae, N. R. Chiquito, Emanuele Gava, E. A. P. Paiva, Paulo Roberto da Silva
ABSTRACT Super sweet corn breeding must develop hybrids that fully meet the expectations of the market. In this sense, the determination of keytraits for the selection of sweetcorn genotypes is a fundamental condition for breeding success. The objective of this work was to identify key traits for the selection of promising and contrasting genotypes of super sweet corn. The experiments were carried out in Guarapuava (PR), in two sowing dates. Seventeen traits related to the yield and quality of the ear were evaluated. To perform the multivariate analysis, data were subjected to diagnosis of multicolinearity, analysis of canonical variables, genetic divergence, hierarchical clusters, factor analysis, and canonical correspondence analysis. Grain yield, yield of dehusked ears, and number of commercial ears were considered related key traits in the identification of promising super sweet corn genotypes. Hybrid D3-20 × D5-41 presented higher averages than the others, considering the yield related keytraits.
超甜玉米育种必须开发出完全符合市场预期的杂交品种。从这个意义上说,确定甜玉米基因型选择的关键性状是育种成功的基本条件。这项工作的目的是确定关键性状,以选择有前途和对比的超甜玉米基因型。试验在瓜拉普瓦(PR)两个播期进行。评价了与穗产量和品质有关的17个性状。为了进行多变量分析,我们对数据进行了多重共线性诊断、典型变量分析、遗传差异、层次聚类、因素分析和典型对应分析。籽粒产量、去皮穗产量和商品穗数被认为是鉴定有潜力的超甜玉米基因型的关键性状。考虑到产量相关关键性状,杂交种D3-20 × D5-41的平均值高于其他品种。
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引用次数: 1
Efficiency of a mine by-product as agricultural liming material and source of zinc 矿山副产品作为农业石灰材料的效率和锌的来源
IF 1.2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-06-04 DOI: 10.1590/1678-4499.20200466
E. Cavalli, A. Lange, L. Teixeira, C. Cavalli, Cleide Aparecida de Abreu, E. Mellis
ABSTRACT Most Brazilian soils have restrictions on agricultural use related to acidity and nutrient availability. This study aims to evaluate a zinc (Zn) and lead (Pb) mining by-product (Zincal™), regarding its capacity to correct soil acidity, and to serve as a source of Zn. For evaluation of its efficacy, a comparison with dolomite + Zn sulfate was conducted. The experiment was set up in two soils, a Quartzarenic Neosol and a Red Latosol, and conducted under controlled conditions in the laboratory. Treatments consisted in the application of two doses, aiming at 75 and 95% base saturation of both liming materials, as well as in a control treatment without acidity correction. The effect of said treatments on pH, base saturation, Al3+, H+Al, Ca2+, Mg2+, Zn, Mn and Pb contents were evaluated over 120 days. Similar responses were obtained from both liming materials, regarding their capacity to increase the pH of the soil, reduce Al3+ and potential acidity (H+Al), and increase base saturation. Differences observed in these attributes were dose-related. In regard to Ca and Mg, the content released in soil correlates with the content present in the liming materials. Zincal™ released more Mg and less Ca than dolomite, hence proving to be efficient in releasing Zinc.
摘要:大多数巴西土壤对农业用途的限制与酸度和养分的可用性有关。本研究旨在评估锌(Zn)和铅(Pb)开采副产品(Zincal™), 关于其校正土壤酸度和作为锌来源的能力。为了评价其疗效,将其与白云石+硫酸锌进行了比较。该实验在两种土壤中进行,一种是石英质新溶胶,另一种是红色Latosol,并在实验室的受控条件下进行。处理包括施用两种剂量,目标是两种石灰材料的75%和95%的碱饱和度,以及在没有酸度校正的对照处理中。在120天内评估了所述处理对pH、碱饱和度、Al3+、H+Al、Ca2+、Mg2+、Zn、Mn和Pb含量的影响。两种石灰材料在提高土壤pH值、降低Al3+和潜在酸度(H+Al)以及增加碱饱和度方面都获得了类似的响应。在这些属性中观察到的差异与剂量有关。就Ca和Mg而言,土壤中释放的含量与石灰材料中的含量相关。Zincal™ 比白云石释放更多的Mg和更少的Ca,因此被证明是有效释放锌的。
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引用次数: 1
Use of winter cover crops improves maize productivity under reduced nitrogen fertilization: a long-term study 一项长期研究表明,在氮肥减少的情况下,使用冬季覆盖作物可提高玉米产量
IF 1.2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-01-15 DOI: 10.1590/1678-4499.20200165
J. Piva, Felipe Bratti, J. L. Locatelli, R. Ribeiro, M. Besen, Eduardo Brancaleoni, D. Schmitt
ABSTRACT The objective was to evaluate the effect of winter cover crops on the agronomic performance of maize during five crop seasons in a clayey Inceptisol with reduced mineral nitrogen (N) inputs. The treatments were seven single cover crops: white oats (Avena sativa L.), black oats (Avena strigose Schreb.), ryegrass (Lolium multiflorum L.), canola (Brassica napus L.), vetch (Vicia sativa L.), fodder radish (Raphanus sativus L. and red clover (Trifolium pratense L.); a mix (black oats + vetch and a fallow. During summer, in succession to the cover crops, maize was grown under no-tillage. The attributes were evaluated in two stages of maize development: flowering (morphological components and physiological maturation (yield components). Differences in morphologica and yield variables were observed in all crop seasons, with emphasis on grain yield, number of grains per row (NGR and ear length (EL). The use of vetch showed greater contribution to the development of maize in relation to the other cover crops used, promoting an increase in stem diameter (SD), E and NGR in all crop season and directly reflecting in higher grain yield. On the other hand, when maize was grown under cover crops commonly used in southern Brazil, such as ryegras and white oats, the yield was similar to fallow. The results showed that vetch promoted higher maize yields, probably by N mineralization and reinforce the hypothesis that cover crops in the off-season can aggregate benefits to crops in succession, especially in terms of nutrition.
摘要本研究旨在评估冬季覆盖作物在减少矿质氮(N)输入的粘性Inceptisol中对五个作物季节玉米农艺性能的影响。处理为7种单覆盖作物:白燕麦(Avena sativa L.)、黑燕麦(Avener strigose Schreb.)、黑麦草(Lolium multiflorum L.),油菜(Brassica napus L.),豌豆(Vicia sativa L..),饲料萝卜(Raphanus sativus L.)和红三叶草(Trifolium pratense L.);一种混合物(黑燕麦+vetch和休耕。在夏季,继覆盖作物之后,玉米在免耕条件下种植。在玉米发育的两个阶段对属性进行了评估:开花(形态成分和生理成熟(产量成分)。在所有作物季节都观察到形态和产量变量的差异,重点是粮食产量、每行粒数(NGR)和穗长(EL)。与使用的其他覆盖作物相比,紫云英的使用对玉米的发育有更大的贡献,在所有作物季节都促进了茎径(SD)、E和NGR的增加,并直接反映出更高的粮食产量。另一方面,当玉米在巴西南部常用的覆盖作物下种植时,如黑麦草和白燕麦,产量与休耕作物相似。结果表明,豌豆可能通过氮矿化促进了玉米产量的提高,并强化了淡季覆盖作物可以为作物带来连续效益的假设,尤其是在营养方面。
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引用次数: 4
Relative competition between white clover and weed species Silene gallica 白三叶草与高卢草的相对竞争
IF 1.2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-01-01 DOI: 10.1590/1678-4499.20200121
Dalvane Rockenbach, A. Balbinot, M. O. Bastiani, F. C. Caratti, D. Agostinetto, F. Lamego
: The aim of the present study was to assess the relative competition between white clover (cv. BRS URS Entrevero) and weed species Silene gallica L. through replacement series. The experiments followed a completely randomized design with four replications. The first study showed that the shoot dry mass of both species (white clover and S. gallica ) became constant with 16 plants.pot -1 or 400 plants.m -2 . In 2017, a study was conducted and it was repeated in 2018 in order to assess white clover competition with S. gallica under different crop:weed proportions pot -1 (100:00; 75:25; 50:50; 25:75 and 00:100). Competitiveness analysis was carried out through diagrams applied to replace experiments and through the relative competition indices. Morphological parameters such as plant height, leaf area and shoot dry mass of the crop and the weed were measured when S. gallica plants bloomed (105 days after species emergence). Results have proven the phenotypic plasticity of temperate-climate foragers such as white clover. This outcome shows that the competitive relationship with Silene gallica changes due to plant proportions composing the association. Therefore, it was possible to conclude that the species show similar competitive ability.
本研究的目的是评估白三叶草(cv。BRS、URS、Entrevero)和高卢草(Silene gallica L.)通过替换系列。实验采用完全随机设计,重复4次。第一项研究表明,白三叶草和高卢草两种植物的茎干质量在16株植物中趋于恒定。1盆或400盆。M -2。2017年进行了一项研究,并于2018年进行了重复研究,以评估不同作物下白三叶草与高卢花的竞争:杂草比例盆栽-1 (100:00;25;50:50;25:75和00:100)。运用图表代替实验,并通过相关竞争指标进行竞争力分析。在高利草开花时(种出后105 d),测定了作物和杂草的株高、叶面积和茎干质量等形态参数。结果证明了白三叶等温带气候觅食动物的表型可塑性。这一结果表明,与高卢硅烯的竞争关系因组成该协会的植物比例而发生变化。因此,有可能得出结论,这两个物种表现出相似的竞争能力。
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引用次数: 0
Nickel-enriched soybean seeds generate plants more resistant to Asian soybean rust 富含镍的大豆种子使植物对亚洲大豆锈病更有抵抗力
IF 1.2 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-01-01 DOI: 10.1590/1678-4499.20200377
S. Ferreira, B. Picanço, B. A. Fontes, A. M. Einhardt, F. Rodrigues
Asian soybean rust (ASR), triggered by Phakopsora pachyrhizi, can cause great yield losses on soybean and nickel (Ni) has potential to control this disease. This study evaluated the effect of using soybean seeds with different Ni contents on ASR control by performing two experiments. In experiment 1, seeds with different Ni contents were obtained by spraying plants three times with solutions containing either 60 or 100 gNi·ha–1. Plants sprayed with water served as the control treatment. In experiment 2, plants originated from seeds obtained in experiment 1 (T1 = 0.9 μgNi·seed–1, T2 = 1.2 μgNi·seed–1, and T3 = 1.6 μgNi·seed–1) were inoculated with P. pachyrhizi at the V4 growth stage. The ASR severity was evaluated 16 days after inoculation (DAI) and the chlorophyll (Chl) a fluorescence parameters at 8 and 16 DAI. The ASR severity decreased by 64 and 47% for treatments T2 and T3, respectively, in comparison to T1 treatment. The photosynthetic apparatus was negatively affected by ASR. The efficiency of the use of light by photosystem II decreased while the energy dissipated by nonregulated form increased in the infected leaflets. There was no significant difference for Chl a fluorescence parameters for T1, T2, and T3 treatments applied to inoculated plants probably due to the biotrophic lifestyle of P. pachyrhizi associated with lower ASR severity. In conclusion, the potential of using seeds with higher Ni content as a tool for ASR integrated management control is highlighted in this study.
亚洲大豆锈病(ASR)是由厚根Phakopsora pachyrhizi引起的大豆锈病,对大豆造成巨大的产量损失,镍(Ni)具有防治潜力。本研究通过两个试验,评价了不同镍含量的大豆种子对ASR的防治效果。在试验1中,用60或100 gNi·ha-1溶液对植株喷施3次,获得不同Ni含量的种子。植物喷水作为对照处理。实验2在实验1获得的种子(T1 = 0.9 μgNi·seed-1, T2 = 1.2 μgNi·seed-1, T3 = 1.6 μgNi·seed-1)的V4生长期接种肿根假单胞菌。测定接种后16 d ASR严重程度(DAI)和接种后8、16 d时叶绿素(Chl) a荧光参数。与T1治疗相比,T2和T3治疗的ASR严重程度分别降低了64%和47%。ASR对光合机构有不利影响。侵染小叶中光系统ⅱ利用光的效率降低,而非调节形式耗散的能量增加。接种植株的T1、T2和T3处理的Chl - a荧光参数无显著差异,这可能是由于肿根菌的生物营养生活方式与ASR严重程度较低有关。综上所述,本研究强调了利用高镍含量种子作为ASR综合管理控制工具的潜力。
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