H. Wegdan, K. Intisar, M. Shaza, O. Algezoli, A. Ballal, H. Ihsan, M. Sahar, A. Baraa, H. Manal, E. Muna, K. Taha, E. M. Nada, Y. H. Ali
{"title":"Serological Detection of Equine Herpes Virus (EHV) Type 1 and 4 in Sudan","authors":"H. Wegdan, K. Intisar, M. Shaza, O. Algezoli, A. Ballal, H. Ihsan, M. Sahar, A. Baraa, H. Manal, E. Muna, K. Taha, E. M. Nada, Y. H. Ali","doi":"10.9734/BMRJ/2016/25803","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/25803","url":null,"abstract":"","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84654644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Igbalajobi, A. Oluyege, A. Oladeji, J. Babalola
Aims: To investigate the prevalence of acquired multidrug resistance of P. aeruginosa among clinical samples obtained from patients attending Ekiti State University Teaching Hospital, Ado Ekiti, Ekiti State, Nigeria. Place and Duration of Study: Ekiti State Teaching Hospital from January-March 2013. Methodology: The isolates were characterized by standard cultural and biochemical tests and they were tested for their sensitivity to different antibiotics using disk diffusion method. Results: A total of 192 clinical samples were collected from which 42 isolates of P. aeruginosa Original Research Article Igbalajobi et al.; BMRJ, 12(4): 1-6, 2016; Article no.BMRJ.22515 2 were obtained. Antibiogram profile showed that a total of 80.95% of the isolates were resistant to ceftriaxone and ceftizoxime respectively, 76.2% to augmentin, 73.8% to ceftazidime, 71.4% to nitrofurantoin, 47.6% to ofloxacin, 45.23% to gentamicin while ciprofloxacin had the lowest resistance of 42.86. Isolates from ear swabs had the highest resistance to 3 generation cephalosporins, followed by isolates from urine while isolates from wound samples showed the lowest resistance. Conclusion: There is a need to institute an effective antimicrobial resistance surveillance system that provides clinicians with up-to-date data on the prevalence and resistance pattern of commonly encountered pathogens like P. aeruginosa especially as nosocomial infection is concerned.
{"title":"Antibiotic Resistance Pattern of Pseudomonas aeruginosa Isolated from Clinical Samples in Ekiti State University Teaching Hospital, Ado-Ekiti, Ekiti State of Nigeria","authors":"O. Igbalajobi, A. Oluyege, A. Oladeji, J. Babalola","doi":"10.9734/bmrj/2016/22515","DOIUrl":"https://doi.org/10.9734/bmrj/2016/22515","url":null,"abstract":"Aims: To investigate the prevalence of acquired multidrug resistance of P. aeruginosa among clinical samples obtained from patients attending Ekiti State University Teaching Hospital, Ado Ekiti, Ekiti State, Nigeria. Place and Duration of Study: Ekiti State Teaching Hospital from January-March 2013. Methodology: The isolates were characterized by standard cultural and biochemical tests and they were tested for their sensitivity to different antibiotics using disk diffusion method. Results: A total of 192 clinical samples were collected from which 42 isolates of P. aeruginosa Original Research Article Igbalajobi et al.; BMRJ, 12(4): 1-6, 2016; Article no.BMRJ.22515 2 were obtained. Antibiogram profile showed that a total of 80.95% of the isolates were resistant to ceftriaxone and ceftizoxime respectively, 76.2% to augmentin, 73.8% to ceftazidime, 71.4% to nitrofurantoin, 47.6% to ofloxacin, 45.23% to gentamicin while ciprofloxacin had the lowest resistance of 42.86. Isolates from ear swabs had the highest resistance to 3 generation cephalosporins, followed by isolates from urine while isolates from wound samples showed the lowest resistance. Conclusion: There is a need to institute an effective antimicrobial resistance surveillance system that provides clinicians with up-to-date data on the prevalence and resistance pattern of commonly encountered pathogens like P. aeruginosa especially as nosocomial infection is concerned.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84745247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Hashemi, F. Fallah, سرور عرفانی منش, Alireza Salimi Chirani, M. Dadashi
Aim : In this study, we evaluated the presence of antibiotic resistance genes among P. aeruginosa strains. Methodology: From January to September 2012, 100 isolates of P. aeruginosa were collected from burn patients. Antimicrobial susceptibility testing was performed by disk diffusion method. Screening for Metallo-β-lactamases (MBLs) productions were performed by Combination Disk Diffusion Test (CDDT). The frequency of antibiotic resistance encoding genes such as MBLs (IMP, VIM, NDM), ESBLs (CTX-M-15), Amp-C enzyme (CMY), Ambler class A carbapenemases (KPC), Ambler class D β-lactamase (OXA-48), 16S rRNA methylases (armA, rmtB, rmtC, rmtD), Quinolone Resistance Gene (aac(6′)-Ib) and class 1 integron were performed by PCR and Sequencing techniques. Results: 48(62.33%) of isolates were metallo-beta-lactamase producers. All MBL-producing Original Research Article Hashemi et al.; BMRJ, 12(4): 1-6, 2016; Article no.BMRJ.23268 2 P. aeruginosa were resistant to antibiotics; while 49% of isolates were resistant to Gentamicin. The aac(6)-Ib, CTX-M-15, int I, CMY, rmtB , rmtD and IMP-1 genes were detected in 57 (74.02%), 48 (62.3%), 48 (62.3%), 7 (9.09%), 11 (14.28%), 9 (11.68%) and 6 (7.7%) isolates respectively, whereas none of them were positive for other genes. The mortality rate due to metallo-βlactamases-producing P. aeruginosa infection was 5(10.4%). Conclusions: The prevalence of antibiotic resistance genes producing P. aeruginosa detected in this study is of great concern.
{"title":"Detection of Antibiotic Resistance Genes among Pseudomonas aeruginosa Strains Isolated from Burn Patients in Iran","authors":"A. Hashemi, F. Fallah, سرور عرفانی منش, Alireza Salimi Chirani, M. Dadashi","doi":"10.9734/bmrj/2016/23268","DOIUrl":"https://doi.org/10.9734/bmrj/2016/23268","url":null,"abstract":"Aim : In this study, we evaluated the presence of antibiotic resistance genes among P. aeruginosa strains. Methodology: From January to September 2012, 100 isolates of P. aeruginosa were collected from burn patients. Antimicrobial susceptibility testing was performed by disk diffusion method. Screening for Metallo-β-lactamases (MBLs) productions were performed by Combination Disk Diffusion Test (CDDT). The frequency of antibiotic resistance encoding genes such as MBLs (IMP, VIM, NDM), ESBLs (CTX-M-15), Amp-C enzyme (CMY), Ambler class A carbapenemases (KPC), Ambler class D β-lactamase (OXA-48), 16S rRNA methylases (armA, rmtB, rmtC, rmtD), Quinolone Resistance Gene (aac(6′)-Ib) and class 1 integron were performed by PCR and Sequencing techniques. Results: 48(62.33%) of isolates were metallo-beta-lactamase producers. All MBL-producing Original Research Article Hashemi et al.; BMRJ, 12(4): 1-6, 2016; Article no.BMRJ.23268 2 P. aeruginosa were resistant to antibiotics; while 49% of isolates were resistant to Gentamicin. The aac(6)-Ib, CTX-M-15, int I, CMY, rmtB , rmtD and IMP-1 genes were detected in 57 (74.02%), 48 (62.3%), 48 (62.3%), 7 (9.09%), 11 (14.28%), 9 (11.68%) and 6 (7.7%) isolates respectively, whereas none of them were positive for other genes. The mortality rate due to metallo-βlactamases-producing P. aeruginosa infection was 5(10.4%). Conclusions: The prevalence of antibiotic resistance genes producing P. aeruginosa detected in this study is of great concern.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91258958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Abd-Elmonsef, Haidy Khalil, A. Selim, S. Abd-Elsalam, W. ElKhalawany, S. Samir, Mohamed Abd-Elghafar, Mohamed M. E. Abd-Elmonsef
Aims: This study aimed to investigate the frequency of hypervirulent Klebsiella pneumoniae (K. pneumoniae) isolated from various hospital-acquired infection cases admitted to Tanta University Hospital, Egypt and to determine the antibiotic resistance profile of these isolates. Study Design: Retrospective observational study. Place and Duration of Study: After collection of K. pneumoniae isolates from microbiology laboratory of Tanta University Hospital. Further work was carried out in the laboratory of Original Research Article Abd-Elmonsef et al.; BMRJ, 17(2): 1-10, 2016; Article no.BMRJ.29240 2 Microbiology and Immunology Department, Faculty of Medicine, Tanta University, Egypt, from June 2015 to May 2016. Methodology: A total of 113 K. pneumoniae isolates were collected from different hospitalacquired infections and were tested for hypermucoviscosity phenotype by string test. Antibiotic disc diffusion test was performed for all isolates to identify their resistance pattern. Existence of rmpA gene was investigated by polymerase chain reaction. Results: Forty-six out of 113 (40.71%) isolates were string test-positive (HVKP), the remaining 67 (59.29%) negative isolates were CKP. Twenty-six (56.52%) out of 46 HVKP isolates possessed rmpA gene. Lower resistance rates were observed in HVKP than CKP. Conclusion: ESBL production by rmpA-positive HVKP isolates in hospital-acquired infections is worrisome, though its rate is still low. Control of the spread of this organism in the hospital environment and the general community is an important concern.
{"title":"Detection of Hypervirulent Klebsiella pneumoniae in Tanta University Hospital, Egypt","authors":"M. Abd-Elmonsef, Haidy Khalil, A. Selim, S. Abd-Elsalam, W. ElKhalawany, S. Samir, Mohamed Abd-Elghafar, Mohamed M. E. Abd-Elmonsef","doi":"10.9734/BMRJ/2016/29240","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/29240","url":null,"abstract":"Aims: This study aimed to investigate the frequency of hypervirulent Klebsiella pneumoniae (K. pneumoniae) isolated from various hospital-acquired infection cases admitted to Tanta University Hospital, Egypt and to determine the antibiotic resistance profile of these isolates. Study Design: Retrospective observational study. Place and Duration of Study: After collection of K. pneumoniae isolates from microbiology laboratory of Tanta University Hospital. Further work was carried out in the laboratory of Original Research Article Abd-Elmonsef et al.; BMRJ, 17(2): 1-10, 2016; Article no.BMRJ.29240 2 Microbiology and Immunology Department, Faculty of Medicine, Tanta University, Egypt, from June 2015 to May 2016. Methodology: A total of 113 K. pneumoniae isolates were collected from different hospitalacquired infections and were tested for hypermucoviscosity phenotype by string test. Antibiotic disc diffusion test was performed for all isolates to identify their resistance pattern. Existence of rmpA gene was investigated by polymerase chain reaction. Results: Forty-six out of 113 (40.71%) isolates were string test-positive (HVKP), the remaining 67 (59.29%) negative isolates were CKP. Twenty-six (56.52%) out of 46 HVKP isolates possessed rmpA gene. Lower resistance rates were observed in HVKP than CKP. Conclusion: ESBL production by rmpA-positive HVKP isolates in hospital-acquired infections is worrisome, though its rate is still low. Control of the spread of this organism in the hospital environment and the general community is an important concern.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90076504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to demonstrate that biogas can be generated from biodegradable domestic wastes and to determine the bacterial succession involved in the anaerobic decomposition of the wastes. Ten kilogram (10 kg) of biodegradable domestic waste was made into slurry with tap water. The slurry was fed into a batch system biodigester and left at room temperature for 12 weeks. Metagenomic method was used to determine the bacterial and archaeal species involved in the anaerobic digestion. MULTIRAE PGM 50 was used to confirm the presence of the generated biogas from the slurry. Serial dilutions of the slurry was made on alternate days and the appropriate dilutions were inoculated onto nutrient agar plates for bacterial isolation and incubation was at 35°C for 48 hrs. Potato dextrose a gar was used for fungal isolation, and incubation was at ambient temperature for three days. Pure isolates of representative communities were maintained on agar slants at 4°C. Triplicate sampl es from various tubes were cultured and the average count was used. Fungal growth occurred on the PDA plate only on the first day of incubation. The mean total bacteriaial count was highest on the second day (1.3 x10 7 cfu/ml); it decreased with increasing incubation time and became constant from the 23 rd day to the end of the experiment (1.0 x10 1 cfu/ml). The microorganisms involved in the biodegradation were found to be Lactobacillus rapi strain LA1165, Clostridyum tyrobutyricum, Ralstonia pickettii, Methanoculleus marisnigri , Methanosarcina acetivorans C2A, Clostridium acetobutylicum EA 2018, Clostridium tyrobutyricum 5S, Halothermothrix oremii H168, Lactobacillus rapi strain LA1165, Lactobacillus buchneri, Solobacterium moorei W540, B. vulgatus ATCC 8482. Rhizopus spp and Aspergillus spp were isolated only on the first two days of incubation. The result from this study proves that, it is possible to generate biogas from domestic wastes and diverse species of microorganisms are involved in anaerobic digestion of biodegradable domestic wastes.
{"title":"Anaerobic Digestion of Biodegradable Domestic Wastes by Microorganisms","authors":"E. Eleanya, C. Wachukwu, A. Ollor","doi":"10.9734/bmrj/2016/21910","DOIUrl":"https://doi.org/10.9734/bmrj/2016/21910","url":null,"abstract":"The aim of this study was to demonstrate that biogas can be generated from biodegradable domestic wastes and to determine the bacterial succession involved in the anaerobic decomposition of the wastes. Ten kilogram (10 kg) of biodegradable domestic waste was made into slurry with tap water. The slurry was fed into a batch system biodigester and left at room temperature for 12 weeks. Metagenomic method was used to determine the bacterial and archaeal species involved in the anaerobic digestion. MULTIRAE PGM 50 was used to confirm the presence of the generated biogas from the slurry. Serial dilutions of the slurry was made on alternate days and the appropriate dilutions were inoculated onto nutrient agar plates for bacterial isolation and incubation was at 35°C for 48 hrs. Potato dextrose a gar was used for fungal isolation, and incubation was at ambient temperature for three days. Pure isolates of representative communities were maintained on agar slants at 4°C. Triplicate sampl es from various tubes were cultured and the average count was used. Fungal growth occurred on the PDA plate only on the first day of incubation. The mean total bacteriaial count was highest on the second day (1.3 x10 7 cfu/ml); it decreased with increasing incubation time and became constant from the 23 rd day to the end of the experiment (1.0 x10 1 cfu/ml). The microorganisms involved in the biodegradation were found to be Lactobacillus rapi strain LA1165, Clostridyum tyrobutyricum, Ralstonia pickettii, Methanoculleus marisnigri , Methanosarcina acetivorans C2A, Clostridium acetobutylicum EA 2018, Clostridium tyrobutyricum 5S, Halothermothrix oremii H168, Lactobacillus rapi strain LA1165, Lactobacillus buchneri, Solobacterium moorei W540, B. vulgatus ATCC 8482. Rhizopus spp and Aspergillus spp were isolated only on the first two days of incubation. The result from this study proves that, it is possible to generate biogas from domestic wastes and diverse species of microorganisms are involved in anaerobic digestion of biodegradable domestic wastes.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88478240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sana Temuri, N. Afzal, M. Kashif, A. Nadeem, F. Shahzad, W. Latif, Afia Abbas, T. Mahmud
Background: Each year, 3 to 4 million people are infected with hepatitis C virus (HCV) and it is the major cause of liver disease worldwide. The patient with acute HCV is often asymptomatic but can present with fatigue and jaundice. About 80% of HCV infected individual’s progress to chronic state. There is an increased prevalence of HCV infection with autoimmune diseases and the most common is chronic thyroiditis, which is an inflammatory disease that leads to hypothyroidism. These individuals have an increased level of antithyroid peroxidase antibody (anti TPO-Ab). Objectives: To determine the prevalence of antithyroid antibody (ATA) in patients of HCV genotype Materials and Methods: Fifty HCV infected patients with genotype 3a were enrolled in this study that included 33 males and 17 females. After written informed consent, 3 ml blood of all the patients was obtained and ATAs were detected by indirect immunofluorescence technique. These patients were divided into 3 groups, i.e. untreated 26 (52%), mid treated 17 (34%) and 7 (14%) patients with hepatocellular carcinoma (HCC). Results: Among the patients 17(51.5%) males and 7 (41.2%) females had ATA. Regarding different groups, 19 (73.1%) of untreated, 5 (29.4%) of mid treated and none of the patient in HCC group had ATA. Conclusion: ATA was detected in a high percentage of patients with HCV genotype 3a. These antibodies were significantly higher in untreated patients as compared to mid treated and HCC patients. Further, more males had these antibodies as compared to females.
{"title":"Anti-thyroid Antibodies in Patients with HCV Genotype 3a: A Pilot Study","authors":"Sana Temuri, N. Afzal, M. Kashif, A. Nadeem, F. Shahzad, W. Latif, Afia Abbas, T. Mahmud","doi":"10.9734/BMRJ/2016/28690","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/28690","url":null,"abstract":"Background: Each year, 3 to 4 million people are infected with hepatitis C virus (HCV) and it is the major cause of liver disease worldwide. The patient with acute HCV is often asymptomatic but can present with fatigue and jaundice. About 80% of HCV infected individual’s progress to chronic state. There is an increased prevalence of HCV infection with autoimmune diseases and the most common is chronic thyroiditis, which is an inflammatory disease that leads to hypothyroidism. These individuals have an increased level of antithyroid peroxidase antibody (anti TPO-Ab). Objectives: To determine the prevalence of antithyroid antibody (ATA) in patients of HCV genotype Materials and Methods: Fifty HCV infected patients with genotype 3a were enrolled in this study that included 33 males and 17 females. After written informed consent, 3 ml blood of all the patients was obtained and ATAs were detected by indirect immunofluorescence technique. These patients were divided into 3 groups, i.e. untreated 26 (52%), mid treated 17 (34%) and 7 (14%) patients with hepatocellular carcinoma (HCC). Results: Among the patients 17(51.5%) males and 7 (41.2%) females had ATA. Regarding different groups, 19 (73.1%) of untreated, 5 (29.4%) of mid treated and none of the patient in HCC group had ATA. Conclusion: ATA was detected in a high percentage of patients with HCV genotype 3a. These antibodies were significantly higher in untreated patients as compared to mid treated and HCC patients. Further, more males had these antibodies as compared to females.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89233288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: The primary aims of this study were to investigate antibiotic resistance patterns and plasmid profile of antibiotic resistant Escherichia coli isolated from clinical specimens, and to find out a possible correlation between plasmids and antibiotic sensitivity patterns. Methodology: Unrelated E. coli strains were isolated from different clinical specimens from different hospitals and primary health care centres in the Riyadh area Saudi Arabia. Antimicrobial susceptibility of E. coli isolates was determined using disc diffusion method against 12 commonly used antimicrobial drugs. Plasmid DNA was extracted from lysed E. coli cells using Plasmid Miniprep kit, and visualised using Agarose gel electrophoresis. Results: The results showed that isolated strains of E. coli were resistant to Cotrimoxazole (70%), Ampicillin (67%), nalidixic acid (51%), Cephalothin (27%), Agumentin and Nitrofurantoin (19%), Tetracycline and Ciprofloxacin (15%) and Gentamycin (12%). Plasmid analysis of clinical isolates showed the presence of 1 to 7 plasmids with size range of 1.9 to 21.1 Kb, as compared to control E. coli ATCC 25922 (size range of 2 to 19.5 Kb). Conclusion: The results obtained in this study showed no direct correlation between the patterns of antibiotic resistance and plasmid profiles.
{"title":"Antibiotic Resistance Patterns and Plasmid Profiles of Escherichia coli Isolates from Clinical Specimens","authors":"A. Jaran","doi":"10.9734/bmrj/2016/22367","DOIUrl":"https://doi.org/10.9734/bmrj/2016/22367","url":null,"abstract":"Aim: The primary aims of this study were to investigate antibiotic resistance patterns and plasmid profile of antibiotic resistant Escherichia coli isolated from clinical specimens, and to find out a possible correlation between plasmids and antibiotic sensitivity patterns. Methodology: Unrelated E. coli strains were isolated from different clinical specimens from different hospitals and primary health care centres in the Riyadh area Saudi Arabia. Antimicrobial susceptibility of E. coli isolates was determined using disc diffusion method against 12 commonly used antimicrobial drugs. Plasmid DNA was extracted from lysed E. coli cells using Plasmid Miniprep kit, and visualised using Agarose gel electrophoresis. Results: The results showed that isolated strains of E. coli were resistant to Cotrimoxazole (70%), Ampicillin (67%), nalidixic acid (51%), Cephalothin (27%), Agumentin and Nitrofurantoin (19%), Tetracycline and Ciprofloxacin (15%) and Gentamycin (12%). Plasmid analysis of clinical isolates showed the presence of 1 to 7 plasmids with size range of 1.9 to 21.1 Kb, as compared to control E. coli ATCC 25922 (size range of 2 to 19.5 Kb). Conclusion: The results obtained in this study showed no direct correlation between the patterns of antibiotic resistance and plasmid profiles.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75002921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Prabakaran, Pranav Pradeep, K. Jeyakumar, Bibin Joseph
{"title":"Antimicrobial Activities of Selected Four Less Known Pulses","authors":"R. Prabakaran, Pranav Pradeep, K. Jeyakumar, Bibin Joseph","doi":"10.9734/bmrj/2016/25596","DOIUrl":"https://doi.org/10.9734/bmrj/2016/25596","url":null,"abstract":"","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76294810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: To develop a simple and rapid detection method for diagnosis of MRSA in bovine milk samples suspected for mastitis. Methodology: The laboratory sensitivity and specificity of LAMP assay was carried out using available laboratory strains. Milk samples were collected from Thiruvallur and Kanchipuram districts of Tamilnadu, India. DNA was directly isolated from the milk samples and mecA gene was screened by both PCR and LAMP methods. Results: The LAMP assay successfully amplified the mecA gene under isothermal conditions at 64°C within 60 min. LAMP assay was able to detect 10 pg of DNA and did not amplify mecA gene from bacterial DNA of other species. The screening of milk samples for MRSA showed 47 Positive out of 77 Samples by PCR and 43 positive out of 77 Samples by LAMP. Conclusion: Application of LAMP assay enabled rapid and easy detection of MRSA in milk samples suspected for bovine mastitis. Short Research Article Sathish et al.; BMRJ, 17(1): 1-5, 2016; Article no.BMRJ.28037 2
目的:建立一种简便、快速的乳腺炎乳样MRSA检测方法。方法:利用现有实验室菌株进行LAMP法的实验室敏感性和特异性分析。牛奶样本采集自印度泰米尔纳德邦的蒂鲁瓦卢尔和坎奇普兰地区。直接从牛奶样品中分离DNA，采用PCR和LAMP方法对mecA基因进行筛选。结果:LAMP法在64°C的等温条件下，在60 min内成功扩增出mecA基因。LAMP法能够检测到10 pg的DNA，并且没有从其他物种的细菌DNA中扩增出mecA基因。77份牛奶样品经PCR检测为阳性47份，经LAMP检测为阳性43份。结论:LAMP法可快速、简便地检测奶牛乳腺炎乳样中的MRSA。研究简述:Sathish et al.;中国生物医学工程学报，17(1):1-5,2016;文章no.BMRJ。28037 2
{"title":"Rapid Detection of MRSA by Loop Mediated Isothermal Amplification in Bovine Milk Samples","authors":"G. Sathish, E. Hemakumar, K. Divya","doi":"10.9734/BMRJ/2016/28037","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/28037","url":null,"abstract":"Aim: To develop a simple and rapid detection method for diagnosis of MRSA in bovine milk samples suspected for mastitis. Methodology: The laboratory sensitivity and specificity of LAMP assay was carried out using available laboratory strains. Milk samples were collected from Thiruvallur and Kanchipuram districts of Tamilnadu, India. DNA was directly isolated from the milk samples and mecA gene was screened by both PCR and LAMP methods. Results: The LAMP assay successfully amplified the mecA gene under isothermal conditions at 64°C within 60 min. LAMP assay was able to detect 10 pg of DNA and did not amplify mecA gene from bacterial DNA of other species. The screening of milk samples for MRSA showed 47 Positive out of 77 Samples by PCR and 43 positive out of 77 Samples by LAMP. Conclusion: Application of LAMP assay enabled rapid and easy detection of MRSA in milk samples suspected for bovine mastitis. Short Research Article Sathish et al.; BMRJ, 17(1): 1-5, 2016; Article no.BMRJ.28037 2","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74667987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim : To study the role of Serum total IgE, serum allergen-specific IgE and Absolute eosinophil count in demonstrating sensitization to various allergens in common atopic disorders. Materials and Methods: A total of sixty one cases with history of atopy in the form of allergic rhinitis, asthma, and urticaria/ dermatitis were subjected to allergy profile test during a period of one year from January 2013- January 2014. The allergy profile test included serum total IgE, serum allergen specific IgE and absolute eosinophil count. Original Research Article
{"title":"Role of Three Different Laboratory Tests in Demonstrating Sensitization to Various Allergens in Common Atopic Disorders","authors":"S. Fatima, N. Aleemuddin, Fakeha Firdous","doi":"10.9734/BMRJ/2016/23105","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/23105","url":null,"abstract":"Aim : To study the role of Serum total IgE, serum allergen-specific IgE and Absolute eosinophil count in demonstrating sensitization to various allergens in common atopic disorders. Materials and Methods: A total of sixty one cases with history of atopy in the form of allergic rhinitis, asthma, and urticaria/ dermatitis were subjected to allergy profile test during a period of one year from January 2013- January 2014. The allergy profile test included serum total IgE, serum allergen specific IgE and absolute eosinophil count. Original Research Article","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72935228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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