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Hand Contamination among Food Handlers 食物处理人员的手污染
Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/24845
H. Allam, M. Al-Batanony, A. Seif, E. Awad
Introduction: Food handlers act as a vehicle for microorganisms causing a potential risk to the public health. Hands contamination of food handlers can be used as an indicator of their behavior regarding food-related practice and personal hygiene. Aim of the Study: To assess hand contamination among food handlers working in Menoufia University and Shebin Al-Kom Educational hospital kitchens and the effect of a health education session over them. Subjects and Methods: A seventy two food handlers were the target group of this study. A prestructured questionnaire was filled including socio-demographic data beside questions regarding risk factors for contamination and personal hygiene practices during dealing with food. Hand rinse sample was taken from each participant to detect the contaminants. A brief and simple health education session was held regarding personal hygiene practices. Three months later, the same kitchen was revisited to appraise the participants’ hygiene compliance where another hand sample rinses were taken. Results: Both Staphylococcus Epidermidis and Escherichia coli (41.7%) followed by Staphylococcus aureus (29.2%) were the prevalent organisms. A significant reduction in hand
导言:食品加工者充当微生物的载体,对公众健康造成潜在风险。食品处理人员的手污染可以作为他们在食品相关实践和个人卫生方面的行为指标。研究目的:评估在Menoufia大学和Shebin Al-Kom教育医院厨房工作的食品处理人员的手污染情况,以及健康教育课程对他们的影响。对象和方法:72名食品处理人员为本研究的目标群体。填写了一份预先编制的问卷,其中包括社会人口统计数据,以及有关污染风险因素和处理食品过程中的个人卫生习惯的问题。从每个参与者身上提取洗手液样本以检测污染物。就个人卫生习惯举行了一次简短的卫生教育会议。三个月后,再次访问同一厨房,评估参与者的卫生依从性,并采取另一种手部样本冲洗。结果:以表皮葡萄球菌和大肠杆菌为主(41.7%),其次是金黄色葡萄球菌(29.2%)。手量显著减少
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引用次数: 17
Extended Spectrum β-lactamases in Clinical Isolates of Escherichia coli and Enterobacter cloacae Collected from Nablus District - Palestine 巴勒斯坦纳布卢斯地区大肠杆菌和阴沟肠杆菌临床分离株β-内酰胺酶的广谱分析
Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/27892
M. Al-Masri, N. Abu-Hasan, Maha Jouhari
Aim: To determine the prevalence of extended-spectrum β-lactamases (ESBLs) among clinical isolates of Escherichia coli (E. coli) and Enterobacter cloacae (E. cloacae) in Nablus district. Methodology: In this prospective study carried out at An-Najah National University, a total of 161 bacterial isolates were collected during a12-month period in Nablus district in Palestine. To detect ESBLs, the isolates were examined by combination disc method. PCR was used to detect blaCTXM, blaTEM and blaSHV genes in 32 representative ESBL-producer E. coli isolates. Results: Using combination disc method, ESBL enzymes were detected in 73 out 153 (47.7%) E. coli and in 1 out of 8 (12.5%) E. cloacae isolates. No significant association of ESBL-producer E. coli was observed with types of collected specimens, gender, hospital ward, outpatient, or medical source. Among 32 representative E. coli ESBL-positive, blaCTX-M, blaTEM and blaSHV genes were detected in 30 (93.8%), 2 (6.3%) and 1 (3.1%), respectively. Two new variants of ESBLs (PALTEM137b and PALSHV-2a') were identified. A unique E. cloacae isolate expressing inducible Short Research Article Al-Masri et al.; BMRJ, 16(3): 1-7, 2016; Article no.BMRJ.27892 2 class C B-lactamase was also detected. Conclusions: In Nablus region, high frequencies of ESBLs were found among E. coli bacteria isolated from outpatients and inpatients. blaCTX-M is the predominant gene among ESBL producers. New variants of ESBLs were found.
目的:了解纳布卢斯地区临床分离的大肠杆菌(E. coli)和阴沟肠杆菌(E. cloacae)中广谱β-内酰胺酶(ESBLs)的流行情况。方法:这项前瞻性研究在najah国立大学进行,在巴勒斯坦纳布卢斯地区12个月期间共收集了161株细菌分离株。采用联合圆盘法检测分离株的ESBLs。采用PCR方法对32株具有代表性的产esbl大肠杆菌进行blaCTXM、blaTEM和blaSHV基因的检测。结果:联合圆盘法在153株大肠杆菌中检出73株(47.7%)ESBL酶,在8株阴沟杆菌中检出1株(12.5%)ESBL酶。未观察到esbl生产者大肠杆菌与采集标本类型、性别、医院病房、门诊或医疗来源有显著关联。在32例esbl阳性的代表性大肠杆菌中,blaCTX-M、blaTEM和blaSHV基因分别检测到30例(93.8%)、2例(6.3%)和1例(3.1%)。鉴定出两种新的ESBLs变体(PALTEM137b和PALSHV-2a’)。一种独特的表达可诱导的阴沟肠杆菌分离株生物医学工程学报,16(3):1-7,2016;文章no.BMRJ。27892 2 C类b -内酰胺酶。结论:纳布卢斯地区门诊和住院患者分离的大肠杆菌中ESBLs的频率较高。blaCTX-M是ESBL生产者的优势基因。发现了esbl的新变体。
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引用次数: 1
Analysis of Influenza A/Beijing/353/89 Virus Matrix Gene Sequence and Identification of Replaceable Amino Acids as Natural Mutations in M2 Protein 甲型流感/北京/353/89病毒基质基因序列分析及M2蛋白自然突变替代氨基酸鉴定
Pub Date : 2016-01-10 DOI: 10.9734/bmrj/2016/27447
Yongxin Zhang, Monica Zhang, Ying Wang, X. Gilmore, I. Mbawuike
The influenza virus A/Beijing/353/89 matrix (M), M1 and M2 genes were cloned and sequenced. The sequence identity rates (based on published data) of the first 34 influenza A viruses with M genes most phylogenetically related to A/Beijing/353/89 virus were in significant positive correlation among M, M1 and M2 genes and M1 protein but not M2 protein. The number of viruses with M2 protein sequences identical to influenza A/Beijing/353/89 virus was significantly higher than those with identical M2 gene and M1 protein sequences (P<0.01). These results suggest that natural variation of M2 protein did not occur entirely in random. Based on a statistical standard we have established and using the A/Beijing/353/89 M2 as reference protein, 35 replaceable amino acids were identified by analysis of 193 published influenza A virus M2 protein sequences. In contrast, it is more possible that those critical amino acids for virus survival and/or replication harbored in the 24 amino acids, which were not yet found to have ever been substituted.
对流感病毒A/Beijing/353/89基质(M)、M1和M2基因进行了克隆和测序。前34株与A/Beijing/353/89病毒系统亲缘关系最密切的M基因A型流感病毒的序列识别率与M、M1、M2基因与M1蛋白呈显著正相关,而与M2蛋白无显著正相关。M2蛋白序列与甲型流感病毒/北京/353/89病毒相同的病毒数量显著高于M2基因和M1蛋白序列相同的病毒数量(P<0.01)。这些结果表明,M2蛋白的自然变异并非完全随机发生的。根据建立的统计标准,以a /Beijing/353/89 M2为参比蛋白,对193个已发表的甲型流感病毒M2蛋白序列进行分析,鉴定出35个可替代氨基酸。相比之下,更有可能的是,那些对病毒存活和/或复制至关重要的氨基酸隐藏在24个氨基酸中,这些氨基酸尚未被发现被取代。
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引用次数: 0
Isolation, Characterization, and Identification of Yeasts Associated with Foods from Assiut City, Egypt 埃及Assiut市食品相关酵母的分离、特性和鉴定
Pub Date : 2016-01-10 DOI: 10.9734/bmrj/2016/24170
S. Khattab, A. Abdel-Hadi, N. Abo-Dahab, O. Atta
The objective of this work was to isolate, characterize physiologically, and identify yeasts associated with foods from Assiut city, Egypt. Fifty-two colonies of yeasts associated with orange, mandarin, tomato, squash, sobia drink, mango juice, sugarcane juice, yogurt and buttermilk samples, collected from Assiut City, Egypt, were isolated. Out of which, Eleven isolates were selected randomly and subjected to morphological, biochemical studies and molecular identification techniques employing sequence of internal transcribed spacer (ITS) regions and partial D1/D2 large-subunit domains of the 26S ribosomal RNA. Identified yeasts were belonged to six genera and species; four species belonged to ascomycetes: Debaryomyces hansenii (five isolates), Saccharomyces cerevisiae (one isolate), Candida tropicalis (one isolate), and Pichia (one isolate). In addition, two yeasts species belonged to basidiomyces: Rhodotorula mucilaginosa (two isolates), and Trichosporon dulcitum (one isolate). In spite of low frequency of yeasts isolates on the tested food, mango juice and buttermilk showed the higher sources for incidences during this study.
这项工作的目的是分离、生理表征和鉴定与埃及Assiut市食物相关的酵母。从埃及Assiut市的橙子、柑橘、番茄、南瓜、啤酒饮料、芒果汁、甘蔗汁、酸奶和酪乳样品中分离出52个菌落。随机选取11株分离株,利用其内部转录间隔区(ITS)序列和部分D1/D2大亚基结构域进行形态学、生化和分子鉴定。经鉴定的酵母菌分属6属、6种;子囊菌属4种,分别为:汉氏Debaryomyces hansenii(5株)、酿酒酵母Saccharomyces cerevisiae(1株)、热带假丝酵母(1株)和毕赤酵母(1株)。另外,担子菌属酵母有2种:粘红酵母(Rhodotorula mucilaginosa)和dulcitum Trichosporon (Trichosporon dulcitum)。尽管在测试食品中分离酵母的频率较低,但在本研究中,芒果汁和酪乳显示出较高的发病率来源。
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引用次数: 8
Lactic Acid Bacteria Composition of Type II Sourdough Produced in Nigeria 尼日利亚产II型酵母的乳酸菌组成
Pub Date : 2016-01-10 DOI: 10.9734/bmrj/2016/16631
M. Dashen, S. Ado, J. Ameh, C. Whong
Aim: The aim of the study was to determine the lactic acid bacteria composition of type II sourdough produced in Nigerian from different brands of wheat flour. Study Design: Sourdoughs were produced by spontaneous fermentation at 31°C and 40°C for five days from three popular brands of Nigerian wheat flour. Lactic acid bacteria were isolated and identified from the sourdoughs using API 50 CH. Methodology: Doughs were prepared from three popular brands of wheat flour and allowed to ferment spontaneously at 31°C and 40°C for five days. Lactic acid bacteria cou nts, fungal counts and aerobic plate counts were carried out. The lactic acid bacteria were identified using the API 50 CH. Data obtained were subjected to statistical analysis using ANOVA. Significant differences among samples were evaluated by Duncan multiple – range test. Results: The results obtained showed that the mean lactic acid bacteria counts were 6.462±0.74, 6.471±0.62 and 6.826±0.68 log CFU/g after five days of fermentation at 31°C while the counts were 6.878±0.99, 6.728±0.95 and 7.051±1.04 log CFU/g after five days of fermentation at 40°C. Lactobacillus plantarum (34%), Lactobacillus brevis (29%) , Lactobacillus pentosus (18%), Pediococcus pentocaseus (9%), Lactobacillus buchneri (3%), Lactobacillus collinoides (3%), Lactobacillus fermentum (3%) and Pediococcus acidilactici (3%) were isolated and identified. Conclusion: There is no known published data on lactic acid bacteria composition of type II sourdough produced in Nigeria; findings of this work will assist to bridge this information gap. Knowledge of the lactic acid bacteria of the sourdough will help in the development of starter cultures for improvement of the nutritional and organoleptic qualities and shelflife of a wide variety of Nigerian baked products.
目的:本研究的目的是测定尼日利亚用不同品牌小麦粉生产的II型酵母的乳酸菌组成。研究设计:采用三种流行品牌的尼日利亚小麦粉,在31°C和40°C条件下自然发酵5天,制成酵母。使用API 50ch从酵母中分离并鉴定乳酸菌。方法:用三种常见品牌的小麦粉制备面团,在31°C和40°C下自行发酵5天。进行乳酸菌计数、真菌计数和好氧平板计数。使用API 50ch鉴定乳酸菌,所得数据采用方差分析进行统计分析。样本间的显著性差异采用Duncan多量程检验。结果:在31℃条件下发酵5 d,乳酸菌平均计数为6.462±0.74、6.471±0.62和6.826±0.68 log CFU/g;在40℃条件下发酵5 d,乳酸菌平均计数为6.878±0.99、6.728±0.95和7.051±1.04 log CFU/g。分离鉴定出植物乳杆菌(34%)、短乳杆菌(29%)、戊酸乳杆菌(18%)、戊酸乳球菌(9%)、布氏乳杆菌(3%)、collinoides乳杆菌(3%)、发酵乳杆菌(3%)和酸碱乳球菌(3%)。结论:目前尚无关于尼日利亚生产的II型酵母中乳酸菌组成的已知公开数据;这项工作的结果将有助于弥合这一信息差距。酵母乳酸菌的知识将有助于开发发酵剂,以改善各种尼日利亚烘焙产品的营养和感官品质以及保质期。
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引用次数: 1
Microbial Assessment of Water Used by the Residents of Kabianga in Kericho, Kenya from the Different Sources 肯尼亚Kericho Kabianga居民从不同来源用水的微生物评估
Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/27557
K. Douglas, J. Mwangi
Aim: The aim of the research was to assess water quality from different sources used by residents of Kabianga. Study Design: The research employed experimental design. Methodology: Techniques used in this research project included test for indicator organism preferable E. coli, total viable count, and enumeration of filamentous fungi and yeasts. For bacteriological quality of the water, indicator organisms were used to indicate the presence of pathogenic microorganisms. Results: The results showed that water obtained from springs and wells were safe for human usage and consumption as it was free from indicators of contamination. The presence of colorless colonies in well water was not considered hazardous because the colonies were well below the Original Research Article Douglas and Mwangi; BMRJ, 16(6): 1-8, 2016; Article no.BMRJ.27557 2 lethal colony number which is set to be a hundred colonies per plate. However the microbial populations identified showed that the river water was contaminated with E. coli an indicator of microbial contamination of water sources. Conclusion: From the findings it can be concluded that river water is likely to be unsafe for use, especially to people who are immunocompromised as they may suffer from diarrheal related diseases. Spontaneous outbreaks related to water-borne diseases in these area, could also be attributed to increase in the number of indicator organisms. There is need for policy makers and implementers to initiate corrective measures to reduce contamination.
目的:本研究的目的是评估Kabianga居民使用的不同水源的水质。研究设计:采用实验设计。方法:本研究项目采用的技术包括指示生物大肠杆菌试验、总活菌计数、丝状真菌和酵母计数。对于水质的细菌学质量,指示生物被用来指示病原微生物的存在。结果:泉水和井水不含污染指标,可安全饮用。井水中无色菌落的存在不被认为是危险的,因为菌落的含量远低于道格拉斯和姆旺吉的原始研究文章;生物医学工程学报,16(6):1-8,2016;文章no.BMRJ。27557 2个致死菌落数设定为每个培养皿100个菌落。然而,微生物种群的鉴定表明,河水受到大肠杆菌的污染,这是水源微生物污染的指标。结论:从研究结果可以得出结论,河水可能不安全,特别是对免疫功能低下的人,因为他们可能患有腹泻相关疾病。这些地区与水媒疾病有关的自发暴发也可归因于指示生物数量的增加。决策者和执行者需要采取纠正措施来减少污染。
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引用次数: 0
A Study of Bacteriological and Physicochemical Characteristics in Soils of Auto-mechanic and none Auto-mechanic Workshop Soils from Selected Areas in Calabar Metropolis 卡拉巴尔市汽车修理厂和非汽车修理厂土壤细菌学和理化特性研究
Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/23596
D. Tiku, B. Asikong, S. Idire
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引用次数: 1
Latent Toxoplasmosis is Not a Risk Factor for Pregnancy-induced Hypertension 潜伏弓形虫病不是妊娠高血压的危险因素
Pub Date : 2016-01-10 DOI: 10.9734/bmrj/2016/23770
A. El-Henawy, H. El-Nahas, Mostafa Alkhiary
Aims: To compare between Toxoplasma IgG antibody seroprevalence in pregnancy-induced hypertensive females (cases) versus normotensive pregnant females (control), and to identify potential risk factors in Toxoplasma infected patients. Study Design: A prospective case-control study. Methodology: We included 78 pregnant females (39 hypertensive, and 39 normotensive; age range 18-39 years). Data concerning demographic and reproductive histories were recorded including previous pregnancies outcome and foetal complications. Comprehensive investigations of the current pregnancy including clinical examination and abdominal ultrasound scan were performed. Five ml venous blood was withdrawn from each female, processed and investigated for the presence of anti- Toxoplasma IgG antibodies by ELISA. Results: The overall Toxoplasma seroprevalence was 40/78 (51.3%) among total participants. Anti- T. gondii IgG antibodies were found in 23/39 (57.5%) of pregnancy-induced hypertension patients and in 17/39 (42.5%) normotensive controls (OR=1.85; 95% CI: 0.7-4.6; P =.17). Of the anti- T. gondii IgG positive patients, 19 (82.6%) had high IgG levels. In comparison only 1 (6.2%) of the anti- T. gondii IgG positive controls showed high IgG levels ( P <.0001). Regarding the specific characteristics of Toxoplasma positive pregnancy-induced hypertension patients, none of those characters displayed a significant correlation with hypertensive tendency except history of abortion ( P =.004). Conclusion: Chronic toxoplasmosis is not a likely risk factor for pregnancy-induced hypertension although significantly higher titre among hypertensive females necessitates further research.
目的:比较妊娠高血压女性(病例)与正常妊娠女性(对照组)弓形虫IgG抗体的血清阳性率,探讨弓形虫感染患者的潜在危险因素。研究设计:前瞻性病例对照研究。方法:我们纳入了78名孕妇(39名高血压,39名正常;年龄18-39岁)。记录有关人口统计学和生殖史的数据,包括以前的妊娠结局和胎儿并发症。对妊娠情况进行全面调查,包括临床检查和腹部超声扫描。每位女性抽取静脉血5 ml,处理后ELISA检测弓形虫IgG抗体。结果:弓形虫血清总阳性率为40/78(51.3%)。妊娠高血压患者中有23/39(57.5%)和正常对照组中有17/39(42.5%)存在抗弓形虫IgG抗体(OR=1.85;95% ci: 0.7-4.6;P =。)。抗弓形虫IgG阳性19例(82.6%)IgG水平较高。而抗刚地弓形虫IgG阳性对照仅有1例(6.2%)IgG水平高(P < 0.0001)。弓形虫阳性妊娠高血压患者的具体特征除有流产史外均与高血压倾向无显著相关性(P = 0.004)。结论:慢性弓形虫病不可能是妊娠高血压的危险因素,但女性高血压患者的滴度明显较高,需要进一步研究。
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引用次数: 2
Dynamics of Nitrogen on Soybean Field Amended with Poultry Manure 鸡粪改良大豆田氮素动态变化
Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/27759
M. Adigun, O. Babalola
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引用次数: 1
Evaluation of Different Screening Methods for Biosurfactant Producers Isolated from Contaminated Egyptian Samples Grown on Industrial Olive Oil Processing Waste 从工业橄榄油加工废料中培养的埃及污染样品中分离生物表面活性剂不同筛选方法的评价
Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/28437
N. Sidkey, H. Mohamed, H. Elkhouly
{"title":"Evaluation of Different Screening Methods for Biosurfactant Producers Isolated from Contaminated Egyptian Samples Grown on Industrial Olive Oil Processing Waste","authors":"N. Sidkey, H. Mohamed, H. Elkhouly","doi":"10.9734/BMRJ/2016/28437","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/28437","url":null,"abstract":"","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":"30 1","pages":"1-19"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88846988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 20
期刊
British microbiology research journal
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