{"title":"Molecular Identification, Bioactivity Screening and Metabolic Fingerprinting of the Actinomycetes of Chenab River Sediments","authors":"M. Cheema, A. Fatima, I. Sajid","doi":"10.9734/bmrj/2016/28805","DOIUrl":"https://doi.org/10.9734/bmrj/2016/28805","url":null,"abstract":"","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73041534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aims: To isolate, characterize and identify surfactant degrading bacteria from selected rivers in Akure, Nigeria and also to compare and quantify the biodegrading potentials of each of the bacterial isolates. Place of Study: Akure metropolis, Ondo state, Nigeria, between June and November, 2013. Methodology: Surfactant degrading bacteria were isolated from the water samples by supplementing culture media with test surfactant. The bacteria isolated were later subjected to the alkylsulphatase enzyme assay to quantify their various enzyme production/activity. Results: The total bacterial load of the water samples range from 7.20±0.69 x10 3 cfu/ml to 40.0±2.31 x10 3 cfu/ml, while the surfactant degrading bacteria counts was within the range of 3.30±0.02 x10 2 cfu/ml to 5.37±2.3 x10 3 cfu/ml. Pseudomonas putida and Exiguobacterium alkylsulphatase surfactant degrading
{"title":"Bacteria Associated with Selected Rivers in Akure, Nigeria and their Alkysulphatase Activity/Production","authors":"D. Arotupin, A. Yusuf","doi":"10.9734/bmrj/2016/23198","DOIUrl":"https://doi.org/10.9734/bmrj/2016/23198","url":null,"abstract":"Aims: To isolate, characterize and identify surfactant degrading bacteria from selected rivers in Akure, Nigeria and also to compare and quantify the biodegrading potentials of each of the bacterial isolates. Place of Study: Akure metropolis, Ondo state, Nigeria, between June and November, 2013. Methodology: Surfactant degrading bacteria were isolated from the water samples by supplementing culture media with test surfactant. The bacteria isolated were later subjected to the alkylsulphatase enzyme assay to quantify their various enzyme production/activity. Results: The total bacterial load of the water samples range from 7.20±0.69 x10 3 cfu/ml to 40.0±2.31 x10 3 cfu/ml, while the surfactant degrading bacteria counts was within the range of 3.30±0.02 x10 2 cfu/ml to 5.37±2.3 x10 3 cfu/ml. Pseudomonas putida and Exiguobacterium alkylsulphatase surfactant degrading","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73181574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bertrand Tatsinkou Fossi, B. Ngah, G. T. Nchanji, S. Wanji, R. Ndjouenkeu
The presence of ochratoxin A (OTA) in cocoa beans is a major health concern, due to its deleterious effects on humans and animals. During the traditional processing and storage of cocoa, fungi contamination occurs. Many of these fungi, produce mycotoxins that can cause acute or chronic intoxication and damage to human and animals after ingestion of the contaminated food and feed. The bio-control of ochratoxigenic moulds by lactic acid bacteria (LAB) could provide a safer alternative compared to the use of fungicides. The present study aims at investigating the potential of selected lactobacilli strains to inhibit the growth of OTA producing moulds. Cocoa samples were collected in the South West region of Cameroon. The mycoflora of cocoa beans were isolated and identified using phenotypic characteristics on Potato Dextrose Agar (PDA) and microscopy after application of lactophenol cotton blue stain. LAB used were isolated from fermented palm and raffia wines and fermented pineapple juice by pour plate method on De Man Rogosa and Sharpe Agar. Phenotypic identification of LAB were identified using API 50 CHL kit. Colony PCR was used to confirm whether the isolates belong to LAB group. The inhibitory activity of Lactobacillus sp against ochratoxigenic moulds was carried out on agar using the overlay method and in broth by turbidimetric analysis. Aspergillus sp were the most prevalent fungi in cocoa samples. Aspergillus flavus , Aspergillus ochraceus, Aspergillus niger were isolated in all samples. Lactobacillus. plantarum exhibited the highest inhibitory activity against OTA producing moulds. The percentage of inhibition was ranged from 10 to 46.5%. A. ochraceus was the most susceptible mould with the various LAB isolates. The quantification of OTA using ELISA kit showed significant reduction in OTA production (p<0.05) during mixed culture of moulds with lactobacilli. Lactobacilli from fermented drinks could be used for biological control of OTA producing fungi.
{"title":"Lactobacilli Cultures against Ochratoxin A Producing Moulds Isolated from Cocoa in the South West Region of Cameroon","authors":"Bertrand Tatsinkou Fossi, B. Ngah, G. T. Nchanji, S. Wanji, R. Ndjouenkeu","doi":"10.9734/BMRJ/2016/25072","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/25072","url":null,"abstract":"The presence of ochratoxin A (OTA) in cocoa beans is a major health concern, due to its deleterious effects on humans and animals. During the traditional processing and storage of cocoa, fungi contamination occurs. Many of these fungi, produce mycotoxins that can cause acute or chronic intoxication and damage to human and animals after ingestion of the contaminated food and feed. The bio-control of ochratoxigenic moulds by lactic acid bacteria (LAB) could provide a safer alternative compared to the use of fungicides. The present study aims at investigating the potential of selected lactobacilli strains to inhibit the growth of OTA producing moulds. Cocoa samples were collected in the South West region of Cameroon. The mycoflora of cocoa beans were isolated and identified using phenotypic characteristics on Potato Dextrose Agar (PDA) and microscopy after application of lactophenol cotton blue stain. LAB used were isolated from fermented palm and raffia wines and fermented pineapple juice by pour plate method on De Man Rogosa and Sharpe Agar. Phenotypic identification of LAB were identified using API 50 CHL kit. Colony PCR was used to confirm whether the isolates belong to LAB group. The inhibitory activity of Lactobacillus sp against ochratoxigenic moulds was carried out on agar using the overlay method and in broth by turbidimetric analysis. Aspergillus sp were the most prevalent fungi in cocoa samples. Aspergillus flavus , Aspergillus ochraceus, Aspergillus niger were isolated in all samples. Lactobacillus. plantarum exhibited the highest inhibitory activity against OTA producing moulds. The percentage of inhibition was ranged from 10 to 46.5%. A. ochraceus was the most susceptible mould with the various LAB isolates. The quantification of OTA using ELISA kit showed significant reduction in OTA production (p<0.05) during mixed culture of moulds with lactobacilli. Lactobacilli from fermented drinks could be used for biological control of OTA producing fungi.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84880721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aims: Xylanase is commonly involved in the complete hydrolysis of xylan which consisted of hemicelluloses the enhancement in amylase activity was also observed in two mutants after UV exposure for 25 minutes and EMS treatment for 60 minutes with the production of 0.481±0.007 U/mL and 0.304±0.040 U/mL compared to wild type with 0.232±0.021 U/mL, respectively. Notably, there were increment of 107% and 31% in activity in mutants compared to wild type. Nevertheless, no possible overproduction of protease and cellulase was detected after the physical and chemical mutagenesis in this study. Conclusion: In a nutshell, the mutants of A. brasiliensis with enhanced production of xylanase and amylase were anticipated to fulfill the industrial demand in more economical approach using agro-residual waste of wheat bran under SmF.
{"title":"Strain Development of Aspergillus brasiliensis Using Physical and Chemicals Mutagenesis for Possible Overproduction of Xylanase, Amylase, Protease and Cellulase under Submerged Fermentation (SmF)","authors":"H. Ho, Muna Abduljubar","doi":"10.9734/bmrj/2016/22953","DOIUrl":"https://doi.org/10.9734/bmrj/2016/22953","url":null,"abstract":"Aims: Xylanase is commonly involved in the complete hydrolysis of xylan which consisted of hemicelluloses the enhancement in amylase activity was also observed in two mutants after UV exposure for 25 minutes and EMS treatment for 60 minutes with the production of 0.481±0.007 U/mL and 0.304±0.040 U/mL compared to wild type with 0.232±0.021 U/mL, respectively. Notably, there were increment of 107% and 31% in activity in mutants compared to wild type. Nevertheless, no possible overproduction of protease and cellulase was detected after the physical and chemical mutagenesis in this study. Conclusion: In a nutshell, the mutants of A. brasiliensis with enhanced production of xylanase and amylase were anticipated to fulfill the industrial demand in more economical approach using agro-residual waste of wheat bran under SmF.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85495391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aflatoxin biosynthesis in Aspergillus flavus requires coordinated expression of regulatory and structural genes. Aflatoxin production is optimum at 24-30°C and inhibition occurs at temperature higher than 35°C. Chaperones or heat-sock proteins are involved in processing of cellular protein and heat-stress induced protein, hence, we studied the genes encoding for heat-shock proteins under the influence of temperature (30°C vs. 37°C). A. flavus isolates, aflatoxigenic (MTCC9367) and atoxigenic (MTCC11580) were grown in glucose minimal salt broth for 24 hours for expression profile of selected genes using quantitative real-time PCR. We monitored the expression profile of genes encoding for heat-shock proteins ( hsp98, hsp90 , hsp70 and hsp60 ) and regulatory gene of aflatoxin biosynthesis pathway aflR . We found the similar trend for heat-shock proteins gene expression except hsp70 in aflatoxigenic and atoxigenic isolates of A. flavus . Expression for hsp70 was found to be upregulated at 30°C (vs 37°C)in atox igenic isolate ( P<0.001) of A. flavus in
黄曲霉毒素的合成需要调控基因和结构基因的协调表达。黄曲霉毒素的最佳产生温度为24-30°C，抑制温度高于35°C。伴侣蛋白或热袜蛋白参与细胞蛋白和热应激诱导蛋白的加工，因此，我们研究了温度(30°C vs. 37°C)影响下热休克蛋白的编码基因。将黄曲霉致黄曲霉毒素(MTCC9367)和抗氧毒素(MTCC11580)分离株在葡萄糖微盐培养液中培养24小时，利用实时荧光定量PCR检测所选基因的表达谱。我们检测了热休克蛋白编码基因(hsp98、hsp90、hsp70和hsp60)和黄曲霉毒素生物合成途径调控基因aflR的表达谱。在黄曲霉毒素和无氧毒素分离株中，除hsp70外，热休克蛋白基因表达趋势相似。hsp70的表达在30°C(相对于37°C)时被发现上调(P<0.001)
{"title":"Differential Expression Pattern of Heat Shock Protein Genes in Toxigenic and Atoxigenic Isolate of Aspergillus flavus","authors":"R. Thakur, S. Tiwari, J. Shankar","doi":"10.9734/BMRJ/2016/25368","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/25368","url":null,"abstract":"Aflatoxin biosynthesis in Aspergillus flavus requires coordinated expression of regulatory and structural genes. Aflatoxin production is optimum at 24-30°C and inhibition occurs at temperature higher than 35°C. Chaperones or heat-sock proteins are involved in processing of cellular protein and heat-stress induced protein, hence, we studied the genes encoding for heat-shock proteins under the influence of temperature (30°C vs. 37°C). A. flavus isolates, aflatoxigenic (MTCC9367) and atoxigenic (MTCC11580) were grown in glucose minimal salt broth for 24 hours for expression profile of selected genes using quantitative real-time PCR. We monitored the expression profile of genes encoding for heat-shock proteins ( hsp98, hsp90 , hsp70 and hsp60 ) and regulatory gene of aflatoxin biosynthesis pathway aflR . We found the similar trend for heat-shock proteins gene expression except hsp70 in aflatoxigenic and atoxigenic isolates of A. flavus . Expression for hsp70 was found to be upregulated at 30°C (vs 37°C)in atox igenic isolate ( P<0.001) of A. flavus in","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85512656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effects of zinc oxide nanoparticles on Escherichia coli and Staphylococcus aureus in deionized water and normal saline, phosphate buffered solution mitigated the inhibitory effect of ZnO nanoparticles on the growth dynamics and population density of E. coli and S. aureus.
{"title":"Diluent Mitigates the Inhibitory Effect of Zinc Oxide Nanoparticles on Escherichia coli and Staphylococcus aureus","authors":"S. Eduok, N. Asamudo","doi":"10.9734/bmrj/2016/23252","DOIUrl":"https://doi.org/10.9734/bmrj/2016/23252","url":null,"abstract":"The effects of zinc oxide nanoparticles on Escherichia coli and Staphylococcus aureus in deionized water and normal saline, phosphate buffered solution mitigated the inhibitory effect of ZnO nanoparticles on the growth dynamics and population density of E. coli and S. aureus.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81144218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Microbiological Examination of Household Kitchen Sponges from Three Communities in Ikwuano L. G. A, Umuahia, Abia State Nigeria","authors":"C. Obi, C. Ndukwu","doi":"10.9734/bmrj/2016/19952","DOIUrl":"https://doi.org/10.9734/bmrj/2016/19952","url":null,"abstract":"","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82898961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shreya Dubey, P. Saini, Chitralekha, Priyanka Singh
Aims: The present study was conducted aiming at the isolation of the pathogenic bacteria from the family enterobacteriaceae in raw, edible and apparently healthy fishes, from different fish markets of Allahabad. Study Design: Forty fish specimens were collected from eight different locations of Allahabad region. Isolation was done using selective plating. Differentiation and characterization of different isolates was based on their growth characteristics on specific culture media, their biochemical confirmatory tests and Gram-staining reactions. Place and Duration of Study: Fish samples were collected from eight different locations of Allahabad during January to May 2016 . Methodology: Isolation of enterobacteriaceae was done according to ISO Standard; ISO 21528-1:2004 and were confirmed by different set of biochemical tests and carbohydrate fermentation tests. Total soluble proteins of the organisms were estimated by Biuret method. Antibiotic susceptibility of the isolates was tested against amphicillin, streptomycin and ciprofloxacin at different concentrations. Results: Strains of Klebsiella planticola , Citrobacter youngae , Citrobacter freundii , E. coli , Klebsiella ornithinolytica and Klebsiella pneumonia were identified out of the 56 isolates obtained. All the isolated strains organisms were susceptible to antibiotics such as amphicillin, streptomycin and ciprofloxacin at different concentrations of 10, 50, 80 and 100 m g/ml. The virulent proteins were highest in K. ornitholytica (103 mg) followed by Citrobacter sp (102.8 mg/ml) . Conclusion: Fishes sold in the fish markets of Allahabad showed presence of pathogenic bacteria especially from the family enterobacteriaceae; indicate poor hygienic conditions as well as improper storage environment. The results concluded that E. coli. (48.20%) represented the major part of bacterial flora, on the fish followed by Klebsiella spp. (30.2%).
{"title":"Prevalence and Antibiotic Susceptibility of Enterobacteriaceae from Fish Samples in Allahabad, India","authors":"Shreya Dubey, P. Saini, Chitralekha, Priyanka Singh","doi":"10.9734/BMRJ/2016/28284","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/28284","url":null,"abstract":"Aims: The present study was conducted aiming at the isolation of the pathogenic bacteria from the family enterobacteriaceae in raw, edible and apparently healthy fishes, from different fish markets of Allahabad. Study Design: Forty fish specimens were collected from eight different locations of Allahabad region. Isolation was done using selective plating. Differentiation and characterization of different isolates was based on their growth characteristics on specific culture media, their biochemical confirmatory tests and Gram-staining reactions. Place and Duration of Study: Fish samples were collected from eight different locations of Allahabad during January to May 2016 . Methodology: Isolation of enterobacteriaceae was done according to ISO Standard; ISO 21528-1:2004 and were confirmed by different set of biochemical tests and carbohydrate fermentation tests. Total soluble proteins of the organisms were estimated by Biuret method. Antibiotic susceptibility of the isolates was tested against amphicillin, streptomycin and ciprofloxacin at different concentrations. Results: Strains of Klebsiella planticola , Citrobacter youngae , Citrobacter freundii , E. coli , Klebsiella ornithinolytica and Klebsiella pneumonia were identified out of the 56 isolates obtained. All the isolated strains organisms were susceptible to antibiotics such as amphicillin, streptomycin and ciprofloxacin at different concentrations of 10, 50, 80 and 100 m g/ml. The virulent proteins were highest in K. ornitholytica (103 mg) followed by Citrobacter sp (102.8 mg/ml) . Conclusion: Fishes sold in the fish markets of Allahabad showed presence of pathogenic bacteria especially from the family enterobacteriaceae; indicate poor hygienic conditions as well as improper storage environment. The results concluded that E. coli. (48.20%) represented the major part of bacterial flora, on the fish followed by Klebsiella spp. (30.2%).","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89593865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Murad Alahdal, Jamal Al-Shabi, Mahmoud Ogaili, Q. Abdullah, S. Alghalibi, A. Jumaan, M. Al-Kamarany
{"title":"Detection of Dengue Fever Virus Serotype – 4 by using One-Step Real-Time RT-PCR in Hodeidah, Yemen","authors":"Murad Alahdal, Jamal Al-Shabi, Mahmoud Ogaili, Q. Abdullah, S. Alghalibi, A. Jumaan, M. Al-Kamarany","doi":"10.9734/BMRJ/2016/24380","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/24380","url":null,"abstract":"","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89982546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Aromolaran, Olubunmi Adesola-Famade, Olayinka Omoseyin
Aim: The aim of this study was to determine the incidence and antibiotic resistant Escherichia coli in Methodology: Fifty two (52) water samples were collected from streams and wells within and around Ondo town. Total aerobic mesophilic and coliform bacteria were determined by standard pour plate and multiple tube fermentation techniques respectively. Escherichia coli was isolated by cultivating on Eosin methylene blue (EMB) agar and tested for resistance to eight antibiotics by Kirby Bauer disc diffusion method. Results: Total mesophilic count in the well and stream water samples were between 0.01 x 10 5 -8.76 x 10 5 cfu/ml and 1.31 x 10 5 - 4.20 x 10 5 cfu/ml respectively. The MPN/100 ml of the water: well (0.40 - >160) and streams (0.70 - >160). E. coli was confirmed present in 67.74% of all the well water and 71.43% of the entire stream. 86.11% of all the isolates were resistant to beta-lactam class of antibiotics, nitrofurantoins (11.11%), aminoglycosides (2.78%) and fluoroquinolones (2.78%). 2.78% were resistant to three classes of antibiotics (nitrofurantoins, aminoglycosides and beta-lactam). 30.56% were resistant to ampicillin, while all the isolates were sensitive to ciprofloxacin. Conclusion: There is need for good hygiene practices and indiscriminate use of antibiotics should be discouraged, in order to reduce the release of antibiotic resistant E. coli to the environment.
{"title":"Incidence of Antibiotic Resistant E. coli Isolated from Drinking Water Sources in Ondo, Southwestern Nigeria","authors":"O. Aromolaran, Olubunmi Adesola-Famade, Olayinka Omoseyin","doi":"10.9734/BMRJ/2016/26876","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/26876","url":null,"abstract":"Aim: The aim of this study was to determine the incidence and antibiotic resistant Escherichia coli in Methodology: Fifty two (52) water samples were collected from streams and wells within and around Ondo town. Total aerobic mesophilic and coliform bacteria were determined by standard pour plate and multiple tube fermentation techniques respectively. Escherichia coli was isolated by cultivating on Eosin methylene blue (EMB) agar and tested for resistance to eight antibiotics by Kirby Bauer disc diffusion method. Results: Total mesophilic count in the well and stream water samples were between 0.01 x 10 5 -8.76 x 10 5 cfu/ml and 1.31 x 10 5 - 4.20 x 10 5 cfu/ml respectively. The MPN/100 ml of the water: well (0.40 - >160) and streams (0.70 - >160). E. coli was confirmed present in 67.74% of all the well water and 71.43% of the entire stream. 86.11% of all the isolates were resistant to beta-lactam class of antibiotics, nitrofurantoins (11.11%), aminoglycosides (2.78%) and fluoroquinolones (2.78%). 2.78% were resistant to three classes of antibiotics (nitrofurantoins, aminoglycosides and beta-lactam). 30.56% were resistant to ampicillin, while all the isolates were sensitive to ciprofloxacin. Conclusion: There is need for good hygiene practices and indiscriminate use of antibiotics should be discouraged, in order to reduce the release of antibiotic resistant E. coli to the environment.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89983595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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