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Transportation conditions of calves upon arrival at major livestock auction markets in Québec, Canada 犊牛抵达加拿大魁北克省主要牲畜拍卖市场时的运输条件
Pub Date : 2024-11-01 DOI: 10.3168/jdsc.2023-0514
Marianne Villettaz Robichaud , Marie-Pascale Morin , Gilles Fecteau , Sébastien Buczinski
The objective of this cross-sectional observational study was to describe the transport conditions of calves at the time of their arrival at the 2 major livestock auction markets in the province of Québec, Canada, and to identify characteristics that affect bedding cleanliness. A particular emphasis was placed on the transport environment of young dairy calves commonly being marketed for veal production. During 4 d per auction site (n = 2 sites), 2 d in summer and 2 d in winter, the descriptive characteristics including type of transports, number of calves per transport, separation from other transported animals, as well as presence of ventilation sources (e.g., open holes allowing natural ventilation), bedding, and bedding cleanliness, were determined. A total of 507 different transports were included, representing a total of 4,054 calves sold during these 8 d. The vast majority of calves (95% [n = 3,845]) were transported by commercially designed trailers (long commercial trailers (n = 358; 70.6% of all transport types), short commercial trailers (n = 62; 12.2%), or multideck trailers (n = 15; 3%). A minority of calves (5%) were either transported by homemade trailers (n = 30; 5.9% of transport) or other types of transports (n = 42; 8.3%). The presence of any ventilation source in the calves' transportation area was observed in 86% of transports and increased in summer versus winter (odds ratio: 2.75 [95% CI: 1.58–4.79]). Bedding was present in 96% of evaluated transports. The majority (68%) of calves' transport flooring area was considered clean, with less than 33% of the calves' area soiled with manure. The dirtiness of calves' transport flooring area was lower in winter than in summer (odds ratio = 0.63 [0.43–0.92]) and in site B than in site A (odds ratio = 0.57 [0.38–0.94]). This study gives interesting insight into transportation and unloading conditions of surplus calves in commercial auction markets.
这项横断面观察研究旨在描述犊牛抵达加拿大魁北克省两大牲畜拍卖市场时的运输条件,并确定影响垫料清洁度的特征。重点是通常用于小牛肉生产的幼乳牛的运输环境。在每个拍卖场(n = 2 个拍卖场)的 4 天(夏季 2 天,冬季 2 天)中,确定了包括运输类型、每次运输的犊牛数量、与其他运输动物的隔离情况以及是否存在通风源(例如允许自然通风的开放式孔洞)、垫料和垫料清洁度在内的描述性特征。绝大多数犊牛(95% [n = 3,845] )是用商业设计的拖车(长商用拖车(n = 358;占所有运输类型的 70.6%)、短商用拖车(n = 62;占 12.2%)或多层拖车(n = 15;占 3%)运输的。少数犊牛(5%)使用自制拖车(n = 30;占运输的 5.9%)或其他类型的运输工具(n = 42;占 8.3%)。在 86% 的运输中观察到犊牛运输区存在任何通风源,且夏季与冬季相比通风源数量增加(几率比:2.75 [95% CI:1.58-4.79])。在 96% 的评估运输中,犊牛都有垫料。大部分(68%)的犊牛运输地板被认为是干净的,只有不到 33% 的犊牛区域被粪便弄脏。犊牛运输地板的脏污程度冬季低于夏季(几率比 = 0.63 [0.43-0.92]),B 区低于 A 区(几率比 = 0.57 [0.38-0.94])。这项研究对商业拍卖市场剩余犊牛的运输和卸载条件提出了有趣的见解。
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引用次数: 0
A simple and robust method for laboratory-scale preparation of butter 实验室规模制备黄油的简单稳健方法
Pub Date : 2024-11-01 DOI: 10.3168/jdsc.2024-0571
Sandra Beyer Gregersen , Lise Margrethe Boesgaard , Dionysios D. Neofytos , Mads Eg Andersen , Ulf Andersen , Milena Corredig
The aim of this study was to develop a small-scale model system resembling the micro- and meso-structure of butter, namely having a water droplet size distribution and water content close to that of commercially produced butter. Although it is possible to churn cream on a small scale, matching the microstructure of butter is a challenge. A 2-step churning process was introduced with the application of a kitchen mixer. The resulting microstructure was evaluated using confocal laser scanning microscopy. In addition, low-field nuclear magnetic resonance was used to determine the water droplet size distribution. Results demonstrated that a water content of 16% to 19% could be obtained with the proposed procedure, close to the standard water content of 16%. Average water droplet size ranged between 2.5 to 4.3 µm and did not depend on batch-to-batch variations, nor storage-induced differences in the cream. In conclusion, the proposed method could be employed to prepare water-in-oil emulsions with a microstructure similar to that of butter and opens new opportunities for studying microbial growth, flavor release, and texture formation.
本研究的目的是开发一种类似于黄油微观和中观结构的小规模模型系统,即水滴大小分布和含水量接近市售黄油。虽然可以在小规模上搅打奶油,但要与黄油的微观结构相匹配却是一项挑战。我们使用厨房搅拌机引入了一个两步搅打过程。使用共焦激光扫描显微镜对由此产生的微观结构进行了评估。此外,还利用低场核磁共振测定了水滴的大小分布。结果表明,采用建议的程序可获得 16% 至 19% 的含水量,接近 16% 的标准含水量。平均水滴大小介于 2.5 至 4.3 微米之间,与批次之间的变化无关,也与奶油的储存差异无关。总之,建议的方法可用于制备具有与黄油相似微观结构的油包水乳剂,并为研究微生物生长、风味释放和质地形成提供了新的机会。
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引用次数: 0
Development of genomic evaluation for methane efficiency in Canadian Holsteins* 研讨会回顾:加拿大荷斯坦牛甲烷效率基因组评估的开发
Pub Date : 2024-11-01 DOI: 10.3168/jdsc.2023-0431
Hinayah Rojas de Oliveira , Hannah Sweett , Saranya Narayana , Allison Fleming , Saeed Shadpour , Francesca Malchiodi , Janusz Jamrozik , Gerrit Kistemaker , Peter Sullivan , Flavio Schenkel , Dagnachew Hailemariam , Paul Stothard , Graham Plastow , Brian Van Doormaal , Michael Lohuis , Jay Shannon , Christine Baes , Filippo Miglior
Reducing methane (CH4) emissions from agriculture, among other sectors, is a key step to reducing global warming. There are many strategies to reduce CH4 emissions in ruminant animals, including genetic selection, which yields cumulative and permanent genetic gains over generations. A single-step genomic evaluation for methane efficiency (MEF) was officially implemented in April 2023 for the Canadian Holstein breed, aiming to reduce CH4 emissions without affecting production levels. This evaluation was achieved by using milk mid-infrared (MIR) spectral data to predict individual cow CH4 production. The genetic evaluation model included milk MIR predicted CH4 (CH4MIR), along with milk yield (MY), fat yield (FY), and protein yield (PY), as correlated traits. Traits were expressed in kilograms per day (MY, FY, and PY) or grams per day (CH4MIR). The MiX99 software was used to fit the single-step, 4-trait animal model. Genomic breeding values for CH4MIR were then obtained by re-parameterization, using recursive genetic linear regression coefficients on MY, FY, and PY, giving a measure of MEF that is genetically independent of the production traits. The estimated breeding values were expressed as relative breeding values with a mean of 100 and standard deviation of 5 for the genetic base population, where a higher value indicates the animal produces lower predicted CH4. This national genomic evaluation is another tool that will lower the dairy industry's carbon footprint by reducing CH4 emissions without affecting production traits.
减少农业和其他行业的甲烷(CH4)排放是减少全球变暖的关键一步。减少反刍动物甲烷(CH4)排放的策略有很多,其中包括基因选择,这种选择可以产生世代累积的永久性基因增益。2023 年 4 月,加拿大荷斯坦品种正式实施了甲烷效率(MEF)单步基因组评估,旨在减少甲烷排放,同时不影响生产水平。这项评估是通过使用牛奶中红外(MIR)光谱数据来预测奶牛个体的甲烷产量来实现的。遗传评估模型包括牛奶中红外预测CH4(CH4MIR),以及作为相关性状的产奶量(MY)、脂肪产量(FY)和蛋白质产量(PY)。性状以公斤/天(MY、FY 和 PY)或克/天(CH4MIR)表示。MiX99 软件用于拟合单步四性状动物模型。然后,通过对 MY、FY 和 PY 的递归遗传线性回归系数重新参数化,得到 CH4MIR 的基因组育种值,从而得到与生产性状无关的 MEF 值。估算的育种值以相对育种值表示,遗传基础种群的平均值为 100,标准偏差为 5,数值越高,表明动物产生的预测 CH4 越低。这项国家基因组评估是在不影响生产性状的情况下减少CH4排放,从而降低奶业碳足迹的又一工具。
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引用次数: 0
Fecal egg counts and individual milk production in temperate pastoral dairy systems of Australia 澳大利亚温带牧区奶牛系统中的粪蛋计数和个体产奶量
Pub Date : 2024-11-01 DOI: 10.3168/jdsc.2024-0555
T. Loughnan , P. Mansell , M. Playford , D. Beggs
Anthelmintic use in lactating dairy cattle has been shown to result in a milk production response in some previous studies. If individual animals within a herd could be identified that would most benefit from anthelmintic treatment, this may reduce anthelmintic resistance. Australian dairy systems are predominantly pasture based, allowing sustained exposure and immune stimulation of cattle to gastrointestinal nematodes. We assessed the relationship between milk production and early-lactation fecal egg counts (FEC) to determine whether cows with higher FEC produced less milk. Ten pasture-based dairy farms in south-west Victoria, Australia, entered an observational study. Individual FEC at a minimum detectable count of 2.5 eggs per gram of feces (epg) were recorded for recently calved primiparous and multiparous cohorts on each farm. All animals were calved ≤30 d at sampling. Body condition scores were assessed at sampling and milk production data were collected from daily milk meters and herd tests to give first 100-d milk production. When separated by primiparous or multiparous status, no difference in the milk production between cows with FEC = 0 and FEC ≥2.5 epg was identified. Between-farm variation was large for FEC and milk production. Fecal egg count at a minimum detectable count of 2.5 epg detected parasitism in primiparous cows postcalving, but the presence and magnitude of parasitism measurable by FEC was not related to milk production in pasture-based Australian dairy systems. In multiparous cows, the rate detection of worm eggs at this analytical sensitivity was lower and the significance of a positive FEC at this analytical sensitivity requires further assessment to ascertain the effect on milk production. Based on our study, it seems unlikely that individual FEC results would be useful as a basis to select individual cows in south-west Victorian dairy herds for anthelmintic treatment.
以前的一些研究表明,对泌乳乳牛使用驱虫药可提高产奶量。如果能确定牛群中哪些动物最受益于驱虫药治疗,就可能减少抗药性。澳大利亚的奶牛饲养系统以牧场为主,这使得牛群能够持续接触胃肠道线虫并受到免疫刺激。我们评估了产奶量与早期泌乳期粪卵计数(FEC)之间的关系,以确定粪卵计数越高的奶牛产奶量是否越少。澳大利亚维多利亚州西南部的十个牧场参与了一项观察性研究。每个牧场都记录了最近产犊的初产牛群和多产牛群的粪便卵计数,每克粪便中至少可检测到 2.5 个卵子(epg)。采样时,所有动物均已产仔≤30 d。采样时对体况评分进行评估,并通过每日奶量计和牛群测试收集产奶量数据,以得出前100天的产奶量。按初产或多产状态区分,FEC = 0 和 FEC ≥2.5 epg 的奶牛的产奶量没有差异。不同牧场之间的FEC和产奶量差异很大。在澳大利亚牧场奶牛系统中,最低可检测到的2.5 epg数量的粪便卵计数可检测到初产奶牛产后的寄生虫,但粪便卵计数可测量的寄生虫的存在和规模与产奶量无关。在多胎奶牛中,这种分析灵敏度下的虫卵检出率较低,在这种分析灵敏度下,FEC阳性的意义需要进一步评估,以确定对产奶量的影响。根据我们的研究,在维多利亚州西南部的奶牛群中,单个 FEC 结果似乎不能作为选择奶牛进行抗蠕虫治疗的依据。
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引用次数: 0
A note from the Editor in Chief of JDS Communications: Our purpose and people JDS Communications 主编的说明:我们的宗旨和员工
Pub Date : 2024-11-01 DOI: 10.3168/jdsc.2024-0680
Jessica A.A. McArt (Editor in Chief)
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引用次数: 0
Mycobacterium bovis vaccination and subsequent experimental infection outcomes are associated with changes in vitamin D status in dairy calves 牛分枝杆菌疫苗接种和随后的实验感染结果与奶牛维生素 D 状态的变化有关。
Pub Date : 2024-11-01 DOI: 10.3168/jdsc.2024-0547
Rachel L. Lee , Kieran G. Meade , Shelley G. Rhodes , Tom Ford , Ilias Kyriazakis
Investigations into the role of vitamin D (vitD) in the immune response of cattle are limited. The objectives of this study were therefore to investigate the association between circulating vitD concentration, tuberculosis (TB) vaccination, and Mycobacterium bovis infection outcomes in 24 dairy calves (<8 wk old) that were housed throughout and fed a BW-based allowance. The study design incorporated 2 phases: vaccination (experimental wk 0–52) and experimental infection phase (wk 52–65). Vaccinated calves (n = 12) received a subcutaneous injection of a live attenuated TB strain at wk 0, whereas unvaccinated ones (n = 12) were injected with saline. All animals were infected with 7,600 cfu of M. bovis 52 wk postvaccination, and lung and lymph node tissues were assessed for pathology following euthanasia after wk 65. Blood samples were taken throughout wk 0 to 65. Serum 25-hydroxyvitamin D [25(OH)D] concentrations were measured using a commercial ELISA. A mixed-effects linear regression model revealed significantly higher 25(OH)D concentrations in the vaccinates postinfection (wk 65) compared with the nonvaccinates. Linear regression analysis between 25(OH)D concentration and the level of M. bovis-driven pathology revealed a negative linear relationship (i.e., higher concentrations were associated with lower pathology scores) irrespective of vaccination status. No correlation was detected between IFN-γ cytokine production and vitD concentration. Overall, the results support an impactful role for vitD in the development of effective immunity of cattle against M. bovis. Gaining insight into the interaction between TB vaccination, M. bovis infection, and vitD could potentially guide the optimization of vaccination protocols and future TB control strategies.
有关维生素 D(vitD)在牛的免疫反应中作用的研究十分有限。因此,本研究的目的是调查 24 头奶牛犊牛(8 周龄)的循环维生素 D 浓度、结核病疫苗接种和牛分枝杆菌感染结果之间的关系。研究设计分为两个阶段:疫苗接种阶段(0-52 周龄的实验阶段)和感染实验阶段(52-65 周龄的实验阶段)。接种疫苗的犊牛(n = 12)在第 0 周皮下注射减毒结核活菌株,而未接种疫苗的犊牛(n = 12)则注射生理盐水。所有动物在接种后第 52 周均感染了 7,600 cfu 的牛海绵状芽孢杆菌,第 65 周后安乐死,对肺部和淋巴结组织进行病理学评估。在第 0 至 65 周期间采集血液样本。血清中 25- 羟基维生素 D [25(OH)D] 的浓度使用商业 ELISA 进行测量。混合效应线性回归模型显示,感染后(第65周)接种疫苗者的25(OH)D浓度明显高于未接种疫苗者。25(OH)D浓度与牛海绵状芽孢杆菌导致的病理水平之间的线性回归分析表明,无论疫苗接种情况如何,两者之间均呈负线性关系(即浓度越高,病理评分越低)。IFN-γ细胞因子的产生与维生素D浓度之间没有相关性。总之,研究结果表明,维生素 D 在牛对牛传染性单核细胞增多症产生有效免疫力方面发挥着重要作用。深入了解结核病疫苗接种、牛海绵状芽孢杆菌感染和 vitD 之间的相互作用可为优化疫苗接种方案和未来的结核病控制策略提供潜在指导。
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引用次数: 0
Effect of an additional gonadotropin-releasing hormone 2 days after the initiation of a resynchronization program 25 days after artificial insemination on fertility of lactating dairy cows 人工授精 25 天后启动再同步化计划,两天后再注射一次 GnRH 对泌乳奶牛繁殖力的影响
Pub Date : 2024-11-01 DOI: 10.3168/jdsc.2023-0540
Iago Matheus Rosa Leão , Anthony Carbajal , César Narciso , Carlos Eduardo Cardoso Consentini , Roberto Sartori , João Paulo Nascimento Martins
We investigated the effect of an additional GnRH 2 d after initiation of resynchronization on d 25 post-AI on the proportion of cows without a corpus luteum (CL) at nonpregnancy diagnosis (NPD) and pregnancy per AI (P/AI) of cows with a CL at NPD that were reinseminated. Once per week, cows that were inseminated 25 d earlier were randomly assigned to receive either a GnRH treatment only (G25) or GnRH on this day and 2 d later (G25&27). Pregnancy diagnosis and CL presence were determined d 32 post-AI using ultrasonography. Nonpregnant cows with a CL were enrolled in different strategies for resynchronization of ovulation according to parity (primiparous or multiparous) and CL size. Primiparous and multiparous cows with a CL ≥20 mm in diameter continued Resynch-25 (PGF on d 32; PGF on d 33; GnRH on d 34; and AI on d 35). Multiparous cows with CL <20 mm restarted a modified Ovsynch at NPD (GnRH – 7 d – PGF – 1 d – PGF – 1 d – GnRH – 1 d – AI). Primiparous cows with CL diameter <20 mm started an Ovsynch+CIDR protocol on the day of NPD (GnRH + CIDR inserted – 7 d – PGF + CIDR removed – 1 d – PGF – 1 d – GnRH – 1 d – AI). Cows without a CL were not enrolled in any resynchronization program for a week and were excluded from fertility analyses. In multiparous cows only, the additional d 27 GnRH treatment was associated with a decreased P/AI 32 d and 102 d post-AI for the AI before treatment. The overall proportion of nonpregnant cows without CL did not differ between treatments. Yet, the GnRH treatment 27 d after AI tended to increase the proportion of overall nonpregnant cows with CL <20 mm at NPD. Treatment did not affect fertility parameters of primiparous cows with a CL ≥20 mm that continued Resynch-25 at NPD. Primiparous cows in G25&27 with CL <20 mm and submitted to Ovsynch+CIDR at NPD tended to have decreased P/AI 102 d after AI and calving/AI. When fertility parameters of primiparous cows with a CL independent of size at NPD were analyzed combined, no effect of treatment was observed. Treatment did not affect fertility of multiparous cows with CL <20 mm enrolled in Ovsynch at NPD. Multiparous cows in the G25&27 group that had a CL ≥20 mm and continued Resynch-25 at NPD tended to have a greater calving/AI due to a decreased total pregnancy loss. In summary, no treatment effect was observed on the overall fertility of primiparous cows with CL at NPD. In multiparous cows, G25&27 was associated with a decreased fertility of the previous AI, but it tended to increase calving/AI for multiparous cows with CL at NPD by decreasing the total pregnancy loss for the AI after treatment.
我们研究了在人工授精后第25天开始再同步化2天后再注射一次GnRH对未孕诊断(NPD)时无黄体(CL)的奶牛比例和NPD时有黄体(CL)的奶牛再人工授精妊娠率(P/AI)的影响。每周一次,将 25 天前授精的奶牛随机分配到只接受 GnRH 治疗(G25)或在这一天和 2 天后接受 GnRH 治疗(G25&27)。人工授精后第 32 天,使用超声波检查确定妊娠诊断和 CL 是否存在。有CL的未孕奶牛根据胎次(初产妇或多胎)和CL大小被纳入不同的排卵再同步策略。CL直径≥20毫米的初产和多产奶牛继续使用Resynch-25(第32天使用PGF2α;第33天使用PGF2α;第34天使用GnRH;第35天人工授精)。CL<20毫米的多胎奶牛在NPD时重新开始改良的卵同步(GnRH - 7 d - PGF2α - 1 d - PGF2α - 1 d - GnRH - 1 d - 人工授精)。CL直径为<20 mm的初产母牛在NPD当天开始卵巢同步+CIDR方案(GnRH + CIDR插入 - 7 d - PGF2α + CIDR移除 - 1 d - PGF2α - 1 d - GnRH - 1 d - 人工授精)。没有CL的奶牛一周内不参加任何再同步化计划,也不进行繁殖力分析。仅在多胎奶牛中,额外的 27 天 GnRH 治疗与治疗前人工授精后 32 天和 102 天的 P/AI 下降有关。无CL的未孕奶牛的总体比例在不同处理之间没有差异。然而,在人工授精后27天进行GnRH治疗往往会增加在NPD时CL <20 mm的未孕奶牛的总体比例。对于在NPD时CL≥20 mm且继续Resynch-25的初产母牛,治疗并不影响其繁殖力参数。G25&27中CL <20毫米的初产母牛在NPD时接受卵同步+CIDR,往往在人工授精后102天P/AI和产犊/AI下降。在对NPD时CL与体型无关的初产母牛的繁殖力参数进行综合分析时,未观察到治疗的影响。在NPD时,CL<20 mm的多胎奶牛参加卵巢综合征,治疗对其繁殖力没有影响。G25&27组中CL≥20毫米的多胎奶牛在NPD时继续使用Resynch-25,由于总妊娠损失减少,产犊/AI往往更高。总之,在NPD时CL的初产母牛的总体繁殖力没有观察到治疗效果。在多胎奶牛中,G25&27与前一次人工授精的受胎率下降有关,但通过降低处理后人工授精的总妊娠损失,G25&27倾向于提高在NPD有CL的多胎奶牛的产犊/AI。
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引用次数: 0
Analysis of gut microbiota with cryptosporidiosis based on fecal condition in neonatal dairy calves on a farm in Japan 根据日本一家农场新生乳牛粪便状况分析隐孢子虫病肠道微生物群
Pub Date : 2024-11-01 DOI: 10.3168/jdsc.2023-0539
Yasuhiro Morita , Momoko Yachida , Keita Tokimitsu, Megumi Itoh
Cryptosporidiosis is a major cause of diarrhea and is associated with high morbidity in calves. Changes in the gut microbiota exacerbate diarrhea caused by Cryptosporidium parvum infection in neonatal and weaned calves. However, information on the gut microbiota of neonatal calves with C. parvum infection is scarce, and research into the microbiome of calves is essential for developing preventive and therapeutic interventions. This study aimed to elucidate the gut microbiota of neonatal calves with cryptosporidiosis. We collected 31 fecal samples from 31 neonatal calves on a dairy farm with or without C. parvum antigen [CP(+) or CP(−)] using a kit and analyzed the differences in the microbiota between diarrheal (D) and normal (N) fecal samples with C. parvum infection based on the fecal score. The analyses revealed the α diversity indexes of fecal microbiota in CP(+)-N samples were higher than that in CP(+)-D samples. Megasphaera spp. and other rumen microbes were identified, and significantly associated with CP(+)-N samples compared with CP(+)-D samples by linear discriminant analysis effect size (LEfSe). We conclude that the specific gut microbiota could characterize fecal microbiota in calves with neonatal cryptosporidiosis without clinical symptoms.
隐孢子虫病是犊牛腹泻的主要病因,发病率很高。肠道微生物群的变化会加剧新生犊牛和断奶犊牛感染副猪嗜隐孢子虫引起的腹泻。然而,有关感染副猪痢疾隐孢子虫的新生犊牛肠道微生物群的信息很少,因此对犊牛微生物群的研究对于开发预防和治疗干预措施至关重要。本研究旨在阐明患有隐孢子虫病的新生犊牛的肠道微生物群。我们使用试剂盒收集了奶牛场中31头感染或未感染隐孢子虫抗原[CP(+)或CP(-)]的新生犊牛的粪便样本,并根据粪便评分分析了感染隐孢子虫的腹泻(D)和正常(N)粪便样本中微生物群的差异。分析结果显示,CP(+)-N样本粪便微生物群的α多样性指数高于CP(+)-D样本。通过线性判别分析效应大小(LEfSe)发现,CP(+)-N 样品与 CP(+)-D 样品相比,与 Megasphaera 属和其他瘤胃微生物显著相关。我们的结论是,特异性肠道微生物群可描述无临床症状的新生儿隐孢子虫病犊牛粪便微生物群的特征。
{"title":"Analysis of gut microbiota with cryptosporidiosis based on fecal condition in neonatal dairy calves on a farm in Japan","authors":"Yasuhiro Morita ,&nbsp;Momoko Yachida ,&nbsp;Keita Tokimitsu,&nbsp;Megumi Itoh","doi":"10.3168/jdsc.2023-0539","DOIUrl":"10.3168/jdsc.2023-0539","url":null,"abstract":"<div><div>Cryptosporidiosis is a major cause of diarrhea and is associated with high morbidity in calves. Changes in the gut microbiota exacerbate diarrhea caused by <em>Cryptosporidium parvum</em> infection in neonatal and weaned calves. However, information on the gut microbiota of neonatal calves with <em>C. parvum</em> infection is scarce, and research into the microbiome of calves is essential for developing preventive and therapeutic interventions. This study aimed to elucidate the gut microbiota of neonatal calves with cryptosporidiosis. We collected 31 fecal samples from 31 neonatal calves on a dairy farm with or without <em>C. parvum</em> antigen [CP(+) or CP(−)] using a kit and analyzed the differences in the microbiota between diarrheal (D) and normal (N) fecal samples with <em>C. parvum</em> infection based on the fecal score. The analyses revealed the α diversity indexes of fecal microbiota in CP(+)-N samples were higher than that in CP(+)-D samples. <em>Megasphaera</em> spp. and other rumen microbes were identified, and significantly associated with CP(+)-N samples compared with CP(+)-D samples by linear discriminant analysis effect size (LEfSe). We conclude that the specific gut microbiota could characterize fecal microbiota in calves with neonatal cryptosporidiosis without clinical symptoms.</div></div>","PeriodicalId":94061,"journal":{"name":"JDS communications","volume":"5 6","pages":"Pages 649-653"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141045934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Birth season affects cow longevity 出生季节影响奶牛寿命
Pub Date : 2024-11-01 DOI: 10.3168/jdsc.2024-0590
I.M. Toledo , L. Cattaneo , J.E.P. Santos , G.E. Dahl
Dairy cow longevity is an important economic trait for producers. In modern dairy farming, longevity of dairy cows is the result of culling decisions, which are determined by several risk factors, including diseases, and reproductive and productive performance. Previous studies have documented that seasonal changes affect health, behavior, and performance of dairy cows throughout their life cycle. Increasing cow comfort by making management adjustments to decrease exposure to high temperatures during the hot months gives farmers the opportunity to decrease culling risk factors and possibly increase cow productive life. In the present study, we obtained the records of primiparous and multiparous Holstein cows from Florida (n = 10,812) and California (n = 8,197) during a 10-yr period (2012–2022). We analyzed the relationship between birth season and longevity (i.e., cows that remained in the herd for 5 or more lactations) in Florida (n = 1,567) and in California (n = 1,669). The number of cows dead, sold, the reasons why they were sold, and their relationship with birth season were also analyzed in the Florida dataset. The hypothesis was that birth in a cool season will increase the length of herd productive life and decrease the number of cows sold or dead during all lactations. The birth seasons were cool (CL; cows born in December, January, February, or March) and hot (HS; cows born in June, July, August, or September). Results showed that in Florida, most cows that remained in the herd for more than 5 lactations (i.e., 14.5% of total cow records) were born during CL compared with cows born during HS (1,129, 72% vs. 438, 28%). The same observations were made in California, where greater longevity (i.e., 5 or more lactations, representing 20.4% of total cow records) was attributed to cows born in CL compared with HS cows (939, 56.3% vs. 730, 43.7%). In Florida, a greater number of HS were sold compared with CL cows (765, 52.6% vs. 689, 47.4%). More HS cows were sold due to breeding, foot and leg, and mastitis issues in Florida. Increased cow death during the first 4 lactations was significantly associated with HS (107, 53.8% vs. 92, 46.2%). Results of this study may help farmers create opportunities to make management adjustments related to birth season, or alter negative seasonal factors (i.e., heat stress) to possibly increase cow longevity in dairy herds.
奶牛长寿对生产者来说是一项重要的经济特征。在现代奶牛养殖业中,奶牛的寿命是淘汰决定的结果,而淘汰决定则由多种风险因素决定,包括疾病、繁殖和生产性能。以往的研究表明,季节变化会影响奶牛整个生命周期的健康、行为和生产性能。通过调整管理,减少奶牛在炎热月份暴露在高温下的时间,从而提高奶牛的舒适度,使奶农有机会减少淘汰奶牛的风险因素,并有可能延长奶牛的生产寿命。在本研究中,我们获得了佛罗里达州(n = 10,812)和加利福尼亚州(n = 8,197)10 年内(2012-2022)初产和多产荷斯坦奶牛的记录。我们分析了佛罗里达州(n = 1,567)和加利福尼亚州(n = 1,669)奶牛出生季节与寿命(即在牛群中存活 5 个或更多泌乳期的奶牛)之间的关系。佛罗里达州的数据集还分析了死亡、出售的奶牛数量、出售原因及其与出生季节的关系。假设是,在凉爽季节出生的奶牛将延长牛群的生产寿命,并减少所有泌乳期中被出售或死亡的奶牛数量。出生季节分为冷季(CL:12 月、1 月、2 月或 3 月出生的奶牛)和热季(HS:6 月、7 月、8 月或 9 月出生的奶牛)。结果显示,在佛罗里达州,大多数在牛群中存活超过 5 个泌乳期的奶牛(占奶牛记录总数的 14.5%)都是在冷季出生的,而在热季出生的奶牛则为 1,129 头,占 72% ;438 头,占 28%。同样的观察结果也出现在加利福尼亚州,与HS期间出生的奶牛相比,CL期间出生的奶牛寿命更长(即5个或5个以上泌乳期,占奶牛记录总数的20.4%)(939头,56.3% vs. 730头,43.7%)。在佛罗里达州,出售的HS奶牛数量多于CL奶牛(765头,52.6%;689头,47.4%)。在佛罗里达州,因繁殖、腿蹄和乳腺炎问题而出售的HS奶牛更多。前4个泌乳期奶牛死亡数的增加与HS有显著关联(107头,53.8%;92头,46.2%)。这项研究的结果可帮助牧场主创造机会,调整与出生季节相关的管理,或改变不利的季节因素(如热应激),从而可能延长奶牛群中奶牛的寿命。
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引用次数: 0
Impacts of feeding milk replacer supplemented with increasing concentrations of choline on feed intake, growth, and scouring incidence for 7 weeks preweaning and 1 week postweaning 在断奶前 7 周和断奶后 1 周内,饲喂添加了浓度不断增加的胆碱的代乳粉对饲料摄入量、生长和冲刷发生率的影响。
Pub Date : 2024-11-01 DOI: 10.3168/jdsc.2024-0557
Anay D. Ravelo , Ellan I. Dufour , Makaila Klejeski , Bruce Ziegler , Angie Golombeski , Isaac J. Salfer
The objective of this study was to evaluate the effect of increasing choline supplementation on the intake of milk replacer (MR) and calf starter (CS), growth performance, and fecal scores of calves over 8 wk. One hundred calves from commercial herds were transported to the University of Minnesota Southern Research and Outreach Center (SROC) calf facility randomized to one of 4 treatments differing in choline supplementation within MR including: 0 (C0; n = 24), 700 (C700; n = 26), 1,400 (C1400; n = 25), and 2,100 mg/kg choline (C2100; n = 25). Milk replacer contained a basal concentration of 1,650 mg/kg MR resulting in total choline concentrations in the MR of 1,650 mg/kg for C0, 2,350 mg/kg for C700, 3,050 mg/kg for C1400, and 3,750 mg/kg for C2100. Calves were weaned at 49 d, and the trial ended at 56 d. Body weight was measured at d 0, 14, 28, 42, 49, and 56. Fecal scores were measured weekly. Feed intake was measured daily and calculated every 2 wk. All feeding, BW measurements, and fecal scoring were conducted by the research staff at the University of Minnesota SROC. Growth, feed intake, and gain to feed ratio were analyzed using a linear mixed effects model with choline concentration as a fixed effect and source herd and room as random effects. Initial BW was included as a covariate for average daily gain. Fecal scores were analyzed as a chi-squared test of significance. Feeding 700 mg/kg supplemental choline increased MR intake throughout the preweaning period and increased ADG in both the preweaning and postweaning periods compared with the other treatments. No differences were observed for fecal scores across treatments. Results suggest that calf performance can be improved with MR containing 2,350 mg/kg choline, contrasting with current NASEM (2021) requirements of 1,000 mg/kg.
:本研究的目的是评估增加胆碱补充量对代乳粉(MR)和犊牛开食料(CS)的摄入量、生长性能以及犊牛 8 周内粪便评分的影响。100 头来自商业牛群的犊牛被运送到明尼苏达大学南方研究与推广中心(SROC)的犊牛饲养场,随机分配到 4 个不同的代乳品胆碱补充剂处理中的一个,包括0(C0;n = 24)、700(C700;n = 26)、1400(C1400;n = 25)和 2100 mg/kg 胆碱(C2100;n = 25)。MR 含有 1650 毫克/千克 MR 的基础浓度,因此 MR 中的总胆碱浓度为:C0 为 1650 毫克/千克,C700 为 2350 毫克/千克,C1400 为 3050 毫克/千克,C2100 为 3750 毫克/千克。犊牛在第 49 天断奶,试验在第 56 天结束。在第 0、14、28、42、49 和 56 天测量体重 (BW)。每周测量粪便评分。每天测量饲料摄入量,每两周计算一次。所有饲喂、体重测量和粪便评分均由明尼苏达大学 SROC 的研究人员进行。采用线性混合效应模型对生长、采食量和增重饲料比进行分析,胆碱浓度为固定效应,来源牛群和房间为随机效应。初始体重作为平均日增重的协变量。粪便评分采用卡方检验进行显著性分析。与其他处理相比,饲喂 700 mg/kg 补充胆碱可增加整个断奶前期的 MR 摄入量,并提高断奶前期和断奶后期的 ADG。各处理的粪便评分无差异。结果表明,使用含 2350 毫克/千克胆碱的 MR 可以提高犊牛的生产性能,而目前 NASEM(2021 年)的要求是 1000 毫克/千克。
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引用次数: 0
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JDS communications
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