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Engineered extracellular vesicles: A new approach for targeted therapy of tumors and overcoming drug resistance 工程细胞外囊泡:肿瘤靶向治疗和克服耐药性的新方法。
IF 16.2 1区 医学 Q1 ONCOLOGY Pub Date : 2023-12-28 DOI: 10.1002/cac2.12518
Chen Ming-Kun, Chen Zi-Xian, Cai Mao-Ping, Chen Hong, Chen Zhuang-Fei, Zhao Shan-Chao

Targeted delivery of anti-tumor drugs and overcoming drug resistance in malignant tumor cells remain significant clinical challenges. However, there are only few effective methods to address these issues. Extracellular vesicles (EVs), actively secreted by cells, play a crucial role in intercellular information transmission and cargo transportation. Recent studies have demonstrated that engineered EVs can serve as drug delivery carriers and showed promising application prospects. Nevertheless, there is an urgent need for further improvements in the isolation and purification of EVs, surface modification techniques, drug assembly processes, and precise recognition of tumor cells for targeted drug delivery purposes. In this review, we summarize the applications of engineered EVs in cancer treatment and overcoming drug resistance, and current challenges associated with engineered EVs are also discussed. This review aims to provide new insights and potential directions for utilizing engineered EVs as targeted delivery systems for anti-tumor drugs and overcoming drug resistance in the near future.

抗肿瘤药物的靶向给药和克服恶性肿瘤细胞的耐药性仍然是重大的临床挑战。然而,目前只有少数几种有效方法可以解决这些问题。细胞外囊泡(EVs)是细胞主动分泌的物质,在细胞间信息传递和货物运输中起着至关重要的作用。最近的研究表明,工程化的EVs可作为药物递送载体,并显示出广阔的应用前景。然而,EVs 的分离和纯化、表面修饰技术、药物组装过程以及肿瘤细胞的精确识别等方面都亟待进一步改进,以达到靶向给药的目的。在这篇综述中,我们总结了工程化 EVs 在癌症治疗和克服耐药性方面的应用,并讨论了当前与工程化 EVs 相关的挑战。本综述旨在为在不久的将来利用工程EVs作为抗肿瘤药物的靶向递送系统和克服耐药性提供新的见解和潜在方向。
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引用次数: 0
Association of previously irradiated stable brain metastases with outcomes of atezolizumab-treated non-small cell lung cancer: A pooled analysis of individual patient data from three randomized trials 曾接受过放射治疗的稳定脑转移灶与阿特珠单抗治疗非小细胞肺癌疗效的关系:对三项随机试验中单个患者数据的汇总分析。
IF 16.2 1区 医学 Q1 ONCOLOGY Pub Date : 2023-12-25 DOI: 10.1002/cac2.12512
Tiantian Guo, Yue Zhou, Fei Liang, Zezhou Wang, Vincent Bourbonne, Lukas Käsmann, Nora Sundahl, Abraham Jing-Ching Wu, Jianjiao Ni, Zhengfei Zhu
<p>Brain metastasis (BM) has long been recognized as a prognostic factor associated with poor prognosis for non-small cell lung cancer (NSCLC) in the era of conventional chemotherapy and targeted therapy [<span>1</span>]. In the era of immunotherapy, controversial findings have been reported regarding the prognostic significance of BM in patients with NSCLC treated with programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) inhibitors. Several studies have shown that the presence of BM did not impact overall survival (OS) or progression-free survival (PFS) [<span>2, 3</span>], whereas other studies have identified BM as a negative prognostic factor [<span>4, 5</span>]. These previous works were mostly based on small sample sizes, and the prognostic significance of BM in patients treated with PD-1/PD-L1 inhibitors warrants further investigation.</p><p>In the present study, we used Vivli, a global, neutral data-sharing platform that enables access to anonymized individual patient data from trials, to evaluate the association between previously irradiated stable BM (iBM) and treatment outcomes of atezolizumab-containing regimens using pooled data from prospective phase III trials. Three clinical trials, IMpower130 (NCT02367781) [<span>6</span>], IMpower131 (NCT02367794) [<span>7</span>], and IMpower150 (NCT02366143) [<span>8</span>], were identified. Supplementary Table S1 provides an overview of the included three clinical trials. The study design and methods are described in the Supplementary Material.</p><p>Supplementary Figure S1 shows the patient disposition for the per-protocol population. In the per-protocol population (<i>n</i> = 2,700), only 10 (3.3%) of 316 patients with baseline BM did not undergo previous irradiation, who were excluded due to difficulties in statistical analyses. Among the 2,690 patients finally included, 210 (11.8%) of 1,778 patients in the atezolizumab-containing arm and 96 (10.5%) of 912 patients in the chemotherapy alone arm had iBM. Baseline demographics and clinical characteristics for patients without baseline BM and patients with iBM are shown in Supplementary Table S2. In patients without BM, OS and PFS were improved with atezolizumab-containing regimens compared with chemotherapy alone (Supplementary Figure S2). In patients with iBM, adding atezolizumab significantly improved OS and PFS compared with chemotherapy alone (Supplementary Figure S3).</p><p>We utilized propensity score matching (PSM) to control for the heterogeneity between patients with iBM and those without BM (Supplementary Table S3). A total of 11 patients in the atezolizumab-containing arm and 10 in the chemotherapy alone arm were excluded due to missing relevant baseline characteristic data. We compared the OS of patients with iBM and those without baseline BM before and after PSM (Figure 1). In the atezolizumab-containing arm, OS was longer in patients with iBM than in those without BM in the original cohort (unadjusted hazard ratio [HR] =
在传统化疗和靶向治疗时代,脑转移(BM)一直被认为是与非小细胞肺癌(NSCLC)不良预后相关的预后因素[1]。在免疫疗法时代,关于程序性死亡-1/程序性死亡配体 1(PD-1/PD-L1)抑制剂治疗的 NSCLC 患者 BM 的预后意义,已有争议性的研究结果。一些研究表明,BM 的存在并不影响总生存期(OS)或无进展生存期(PFS)[2, 3],而另一些研究则认为 BM 是一个负面的预后因素[4, 5]。在本研究中,我们使用 Vivli(一个全球性、中立的数据共享平台,可访问试验中匿名的患者个体数据),利用前瞻性 III 期试验的汇总数据评估了既往照射过的稳定 BM(iBM)与含阿特珠单抗方案治疗结果之间的关联。共确定了三项临床试验:IMpower130(NCT02367781)[6]、IMpower131(NCT02367794)[7]和IMpower150(NCT02366143)[8]。补充表 S1 提供了所纳入的三项临床试验的概况。研究设计和方法见补充材料。补充图 S1 显示了按协议人群的患者处置情况。在按协议人群(n = 2,700)中,基线BM的316名患者中仅有10人(3.3%)既往未接受过照射,由于统计分析困难而被排除在外。在最终纳入的 2,690 名患者中,含阿替珠单抗治疗组的 1,778 名患者中有 210 人(11.8%)患有 iBM,而单纯化疗组的 912 名患者中有 96 人(10.5%)患有 iBM。无基线BM患者和有iBM患者的基线人口统计学特征和临床特征见补充表S2。与单纯化疗相比,在无基础骨髓瘤的患者中,使用含有阿特珠单抗的方案可改善OS和PFS(补充图S2)。在iBM患者中,与单纯化疗相比,添加atezolizumab能显著改善OS和PFS(补充图S3)。我们利用倾向评分匹配(PSM)来控制iBM患者与无BM患者之间的异质性(补充表S3)。由于缺失相关基线特征数据,共排除了含阿替珠单抗治疗组的11例患者和单纯化疗组的10例患者。我们比较了iBM患者和无基线BM患者在PSM前后的OS(图1)。在含有阿特珠单抗的治疗组中,原始队列中的iBM患者的OS长于无BM患者(未经调整的危险比[HR] = 0.75,95%置信区间[CI] = 0.60-0.93,P = 0.011;调整后的HR = 0.75,95% CI = 0.60-0.95,P = 0.015;补充表S4),倾向评分匹配队列中的iBM患者的OS也长于无BM患者(未经调整的HR = 0.35, 95% CI = 0.27-0.45, P &lt; 0.001; 调整后 HR = 0.26, 95% CI = 0.20-0.33, P &lt; 0.001; 补充表 S5);在单纯化疗组中,原始队列和倾向评分匹配队列中的 iBM 患者与无 BM 患者的 OS 相似。在 PSM 前后,治疗组与 BM 状态之间存在明显的交互作用(Pinteraction = 0.013,Pinteraction &lt; 0.001)。我们还应用了稳定的反概率加权法,得到了相似的结果(补充表 S6 和补充图 S4)。我们还比较了有 iBM 和无基线 BM 患者的 PFS,结果见补充图 S5-S6 和补充表 S7-S10。此外,我们还比较了无BM患者和有放疗技术信息的iBM患者的OS和PFS,发现放射外科治疗的稳定BM或全脑放疗治疗的稳定BM与PSM后含阿特珠单抗组生存率的改善有关,但与单纯化疗组无关(补充图S7-S10)。225例(14.3%)无基线BM的患者和31例(14.8%)iBM患者发生了任何级别的阿特珠单抗相关神经系统不良事件(AEs);19例(1.2%)无基线BM的患者和4例(1.9%)iBM患者发生了阿特珠单抗相关的3/4级神经系统不良事件(补充表S11)。无BM患者和iBM患者发生atezolizumab相关严重神经系统AEs的比例相似(0.7% vs. 0.4%)。最常见的atezolizumab相关神经系统AE是无基线BM患者的头痛和iBM患者的周围神经病变(补充表S12)。
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引用次数: 0
Multiomics analysis reveals metabolic subtypes and identifies diacylglycerol kinase α (DGKA) as a potential therapeutic target for intrahepatic cholangiocarcinoma 多组学分析揭示了代谢亚型,并确定二酰甘油激酶α(DGKA)是肝内胆管癌的潜在治疗靶点。
IF 16.2 1区 医学 Q1 ONCOLOGY Pub Date : 2023-12-24 DOI: 10.1002/cac2.12513
Weiren Liu, Huqiang Wang, Qianfu Zhao, Chenyang Tao, Weifeng Qu, Yushan Hou, Run Huang, Zimei Sun, Guiqi Zhu, Xifei Jiang, Yuan Fang, Jun Gao, Xiaoling Wu, Zhixiang Yang, Rongyu Ping, Jiafeng Chen, Rui Yang, Tianhao Chu, Jian Zhou, Jia Fan, Zheng Tang, Dong Yang, Yinghong Shi

Background

Intrahepatic cholangiocarcinoma (iCCA) is a highly heterogeneous and lethal hepatobiliary tumor with few therapeutic strategies. The metabolic reprogramming of tumor cells plays an essential role in the development of tumors, while the metabolic molecular classification of iCCA is largely unknown. Here, we performed an integrated multiomics analysis and metabolic classification to depict differences in metabolic characteristics of iCCA patients, hoping to provide a novel perspective to understand and treat iCCA.

Methods

We performed integrated multiomics analysis in 116 iCCA samples, including whole-exome sequencing, bulk RNA-sequencing and proteome analysis. Based on the non-negative matrix factorization method and the protein abundance of metabolic genes in human genome-scale metabolic models, the metabolic subtype of iCCA was determined. Survival and prognostic gene analyses were used to compare overall survival (OS) differences between metabolic subtypes. Cell proliferation analysis, 5-ethynyl-2'-deoxyuridine (EdU) assay, colony formation assay, RNA-sequencing and Western blotting were performed to investigate the molecular mechanisms of diacylglycerol kinase α (DGKA) in iCCA cells.

Results

Three metabolic subtypes (S1-S3) with subtype-specific biomarkers of iCCA were identified. These metabolic subtypes presented with distinct prognoses, metabolic features, immune microenvironments, and genetic alterations. The S2 subtype with the worst survival showed the activation of some special metabolic processes, immune-suppressed microenvironment and Kirsten rat sarcoma viral oncogene homolog (KRAS)/AT-rich interactive domain 1A (ARID1A) mutations. Among the S2 subtype-specific upregulated proteins, DGKA was further identified as a potential drug target for iCCA, which promoted cell proliferation by enhancing phosphatidic acid (PA) metabolism and activating mitogen-activated protein kinase (MAPK) signaling.

Conclusion

Via multiomics analyses, we identified three metabolic subtypes of iCCA, revealing that the S2 subtype exhibited the poorest survival outcomes. We further identified DGKA as a potential target for the S2 subtype.

背景:肝内胆管癌(iCCA)是一种高度异质性的致死性肝胆肿瘤,治疗策略很少。肿瘤细胞的代谢重编程在肿瘤的发展过程中起着至关重要的作用,而 iCCA 的代谢分子分类在很大程度上是未知的。在此,我们进行了综合多组学分析和代谢分类,以描述iCCA患者代谢特征的差异,希望能为理解和治疗iCCA提供一个新的视角:我们对116份iCCA样本进行了综合多组学分析,包括全外显子组测序、大量RNA测序和蛋白质组分析。根据非负矩阵因式分解法和人类基因组尺度代谢模型中代谢基因的蛋白质丰度,确定了iCCA的代谢亚型。生存和预后基因分析用于比较不同代谢亚型的总生存率(OS)差异。通过细胞增殖分析、5-乙炔基-2'-脱氧尿苷(EdU)检测、集落形成检测、RNA测序和Western印迹等方法研究了iCCA细胞中二酰甘油激酶α(DGKA)的分子机制:结果:发现了三种代谢亚型(S1-S3),它们具有iCCA亚型特异性生物标志物。这些代谢亚型具有不同的预后、代谢特征、免疫微环境和基因改变。生存率最差的S2亚型表现出一些特殊代谢过程的激活、免疫抑制微环境和Kirsten大鼠肉瘤病毒癌基因同源体(KRAS)/富AT交互结构域1A(ARID1A)突变。在S2亚型特异性上调蛋白中,DGKA被进一步鉴定为iCCA的潜在药物靶点,它通过增强磷脂酸(PA)代谢和激活丝裂原活化蛋白激酶(MAPK)信号传导促进细胞增殖:通过多组学分析,我们确定了iCCA的三种代谢亚型,发现S2亚型的生存率最差。我们进一步确定了DGKA是S2亚型的潜在靶点。
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引用次数: 0
Rescue of p53 functions by in vitro-transcribed mRNA impedes the growth of high-grade serous ovarian cancer 体外转录的 mRNA 对 p53 功能的修复阻碍了高级别浆液性卵巢癌的生长。
IF 16.2 1区 医学 Q1 ONCOLOGY Pub Date : 2023-12-22 DOI: 10.1002/cac2.12511
Monika Raab, Izabela Kostova, Samuel Peña-Llopis, Daniela Fietz, Monika Kressin, Seyed Mohsen Aberoumandi, Evelyn Ullrich, Sven Becker, Mourad Sanhaji, Klaus Strebhardt
<div> <section> <h3> Background</h3> <p>The cellular tumor protein p53 (<i>TP53</i>) is a tumor suppressor gene that is frequently mutated in human cancers. Among various cancer types, the very aggressive high-grade serous ovarian carcinoma (HGSOC) exhibits the highest prevalence of <i>TP53</i> mutations, present in >96% of cases. Despite intensive efforts to reactivate p53, no clinical drug has been approved to rescue p53 function. In this study, our primary objective was to administer in vitro-transcribed (IVT) wild-type (WT) p53-mRNA to HGSOC cell lines, primary cells, and orthotopic mouse models, with the aim of exploring its impact on inhibiting tumor growth and dissemination, both in vitro and in vivo.</p> </section> <section> <h3> Methods</h3> <p>To restore the activity of p53, WT p53 was exogenously expressed in HGSOC cell lines using a mammalian vector system. Moreover, IVT WT p53 mRNA was delivered into different HGSOC model systems (primary cells and patient-derived organoids) using liposomes and studied for proliferation, cell cycle progression, apoptosis, colony formation, and chromosomal instability. Transcriptomic alterations induced by p53 mRNA were analyzed using RNA sequencing in OVCAR-8 and primary HGSOC cells, followed by ingenuity pathway analysis. In vivo effects on tumor growth and metastasis were studied using orthotopic xenografts and metastatic intraperitoneal mouse models.</p> </section> <section> <h3> Results</h3> <p>Reactivation of the <i>TP53</i> tumor suppressor gene was explored in different HGSOC model systems using newly designed IVT mRNA-based methods. The introduction of WT p53 mRNA triggered dose-dependent apoptosis, cell cycle arrest, and potent long-lasting inhibition of HGSOC cell proliferation. Transcriptome analysis of OVCAR-8 cells upon mRNA-based p53 reactivation revealed significant alterations in gene expression related to p53 signaling, such as apoptosis, cell cycle regulation, and DNA damage. Restoring p53 function concurrently reduces chromosomal instability within the HGSOC cells, underscoring its crucial contribution in safeguarding genomic integrity by moderating the baseline occurrence of double-strand breaks arising from replication stress. Furthermore, in various mouse models, treatment with p53 mRNA reduced tumor growth and inhibited tumor cell dissemination in the peritoneal cavity in a dose-dependent manner.</p> </section> <section> <h3> Conclusions</h3> <p>The IVT mRNA-based reactivation of p53 holds promise as a potential therapeutic strategy for HGSOC, providing valuable insights into
背景:细胞肿瘤蛋白 p53(TP53)是一种抑癌基因,在人类癌症中经常发生突变。在各种癌症类型中,侵袭性极强的高级别浆液性卵巢癌(HGSOC)的 TP53 基因突变发生率最高,超过 96% 的病例都存在这种突变。尽管人们为重新激活 p53 付出了巨大努力,但目前尚未批准任何临床药物来挽救 p53 的功能。在本研究中,我们的主要目的是向 HGSOC 细胞系、原代细胞和小鼠模型中注入体外转录(IVT)的野生型 p53-mRNA,以探索其对抑制肿瘤在体外和体内生长和扩散的影响:为了恢复 p53 的活性,使用哺乳动物载体系统在 HGSOC 细胞系中外源表达 WT p53。此外,利用脂质体将 IVT WT p53 mRNA 运送到不同的 HGSOC 模型系统(原代细胞和患者衍生的器官组织)中,并对其增殖、细胞周期进展、细胞凋亡、集落形成和染色体不稳定性进行研究。使用 RNA 测序分析了 p53 mRNA 在 OVCAR-8 和原代 HGSOC 细胞中诱导的转录组变化,随后进行了巧妙通路分析。使用正位异种移植和腹腔转移小鼠模型研究了体内对肿瘤生长和转移的影响:结果:利用新设计的基于 IVT mRNA 的方法,在不同的 HGSOC 模型系统中探索了 TP53 抑癌基因的重新激活。引入 WT p53 mRNA 会引发剂量依赖性凋亡、细胞周期停滞以及对 HGSOC 细胞增殖的强效持久抑制。基于 mRNA 的 p53 重新激活后,OVCAR-8 细胞的转录组分析显示,与 p53 信号转导相关的基因表达发生了显著变化,如细胞凋亡、细胞周期调控和 DNA 损伤。恢复 p53 功能的同时还降低了 HGSOC 细胞内染色体的不稳定性,强调了它在保护基因组完整性方面的重要贡献,因为它能缓和复制应激引起的双链断裂的基线发生。此外,在各种小鼠模型中,使用 p53 mRNA 治疗可减少肿瘤生长,并以剂量依赖的方式抑制肿瘤细胞在腹腔中的扩散:基于 IVT mRNA 的 p53 重激活有望成为 HGSOC 的一种潜在治疗策略,为 p53 功能的分子机制及其在卵巢癌治疗中的相关性提供了宝贵的见解。
{"title":"Rescue of p53 functions by in vitro-transcribed mRNA impedes the growth of high-grade serous ovarian cancer","authors":"Monika Raab,&nbsp;Izabela Kostova,&nbsp;Samuel Peña-Llopis,&nbsp;Daniela Fietz,&nbsp;Monika Kressin,&nbsp;Seyed Mohsen Aberoumandi,&nbsp;Evelyn Ullrich,&nbsp;Sven Becker,&nbsp;Mourad Sanhaji,&nbsp;Klaus Strebhardt","doi":"10.1002/cac2.12511","DOIUrl":"10.1002/cac2.12511","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Background&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;The cellular tumor protein p53 (&lt;i&gt;TP53&lt;/i&gt;) is a tumor suppressor gene that is frequently mutated in human cancers. Among various cancer types, the very aggressive high-grade serous ovarian carcinoma (HGSOC) exhibits the highest prevalence of &lt;i&gt;TP53&lt;/i&gt; mutations, present in &gt;96% of cases. Despite intensive efforts to reactivate p53, no clinical drug has been approved to rescue p53 function. In this study, our primary objective was to administer in vitro-transcribed (IVT) wild-type (WT) p53-mRNA to HGSOC cell lines, primary cells, and orthotopic mouse models, with the aim of exploring its impact on inhibiting tumor growth and dissemination, both in vitro and in vivo.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;To restore the activity of p53, WT p53 was exogenously expressed in HGSOC cell lines using a mammalian vector system. Moreover, IVT WT p53 mRNA was delivered into different HGSOC model systems (primary cells and patient-derived organoids) using liposomes and studied for proliferation, cell cycle progression, apoptosis, colony formation, and chromosomal instability. Transcriptomic alterations induced by p53 mRNA were analyzed using RNA sequencing in OVCAR-8 and primary HGSOC cells, followed by ingenuity pathway analysis. In vivo effects on tumor growth and metastasis were studied using orthotopic xenografts and metastatic intraperitoneal mouse models.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Reactivation of the &lt;i&gt;TP53&lt;/i&gt; tumor suppressor gene was explored in different HGSOC model systems using newly designed IVT mRNA-based methods. The introduction of WT p53 mRNA triggered dose-dependent apoptosis, cell cycle arrest, and potent long-lasting inhibition of HGSOC cell proliferation. Transcriptome analysis of OVCAR-8 cells upon mRNA-based p53 reactivation revealed significant alterations in gene expression related to p53 signaling, such as apoptosis, cell cycle regulation, and DNA damage. Restoring p53 function concurrently reduces chromosomal instability within the HGSOC cells, underscoring its crucial contribution in safeguarding genomic integrity by moderating the baseline occurrence of double-strand breaks arising from replication stress. Furthermore, in various mouse models, treatment with p53 mRNA reduced tumor growth and inhibited tumor cell dissemination in the peritoneal cavity in a dose-dependent manner.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Conclusions&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;The IVT mRNA-based reactivation of p53 holds promise as a potential therapeutic strategy for HGSOC, providing valuable insights into ","PeriodicalId":9495,"journal":{"name":"Cancer Communications","volume":"44 1","pages":"101-126"},"PeriodicalIF":16.2,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cac2.12511","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138884486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Glucose-mediated mitochondrial reprogramming by cholesterol export at TM4SF5-enriched mitochondria-lysosome contact sites 在富含 TM4SF5 的线粒体-溶酶体接触点,通过胆固醇输出实现葡萄糖介导的线粒体重编程。
IF 16.2 1区 医学 Q1 ONCOLOGY Pub Date : 2023-12-22 DOI: 10.1002/cac2.12510
Ji Eon Kim, So-Young Park, Chulhwan Kwak, Yoonji Lee, Dae-Geun Song, Jae Woo Jung, Haesong Lee, Eun-Ae Shin, Yangie Pinanga, Kyung-hee Pyo, Eun Hae Lee, Wonsik Kim, Soyeon Kim, Chang-Duck Jun, Jeanho Yun, Sun Choi, Hyun-Woo Rhee, Kwang-Hyeon Liu, Jung Weon Lee

Background

Transmembrane 4 L six family member 5 (TM4SF5) translocates subcellularly and functions metabolically, although it is unclear how intracellular TM4SF5 translocation is linked to metabolic contexts. It is thus of interests to understand how the traffic dynamics of TM4SF5 to subcellular endosomal membranes are correlated to regulatory roles of metabolisms.

Methods

Here, we explored the metabolic significance of TM4SF5 localization at mitochondria-lysosome contact sites (MLCSs), using in vitro cells and in vivo animal systems, via approaches by immunofluorescence, proximity labelling based proteomics analysis, organelle reconstitution etc.

Results

Upon extracellular glucose repletion following depletion, TM4SF5 became enriched at MLCSs via an interaction between mitochondrial FK506-binding protein 8 (FKBP8) and lysosomal TM4SF5. Proximity labeling showed molecular clustering of phospho-dynamic-related protein I (DRP1) and certain mitophagy receptors at TM4SF5-enriched MLCSs, leading to mitochondrial fission and autophagy. TM4SF5 bound NPC intracellular cholesterol transporter 1 (NPC1) and free cholesterol, and mediated export of lysosomal cholesterol to mitochondria, leading to impaired oxidative phosphorylation but intact tricarboxylic acid (TCA) cycle and β-oxidation. In mouse models, hepatocyte Tm4sf5 promoted mitophagy and cholesterol transport to mitochondria, both with positive relations to liver malignancy.

Conclusions

Our findings suggested that TM4SF5-enriched MLCSs regulate glucose catabolism by facilitating cholesterol export for mitochondrial reprogramming, presumably while hepatocellular carcinogenesis, recapitulating aspects for hepatocellular carcinoma metabolism with mitochondrial reprogramming to support biomolecule synthesis in addition to glycolytic energetics.

背景:跨膜4 L 6家族成员5(TM4SF5)在亚细胞内转运并发挥代谢功能,但目前尚不清楚细胞内TM4SF5的转运如何与代谢环境相关联。方法:在此,我们利用体外细胞和体内动物系统,通过免疫荧光、基于接近标记的蛋白质组学分析、细胞器重组等方法,探讨了 TM4SF5 在线粒体-赖氨酸接触位点(MLCSs)定位的代谢意义。结果:通过线粒体 FK506 结合蛋白 8 (FKBP8) 和溶酶体 TM4SF5 之间的相互作用,当细胞外葡萄糖耗竭后,TM4SF5 在 MLCS 上富集。接近标记显示,磷酸化动态相关蛋白 I(DRP1)和某些有丝分裂受体分子聚集在 TM4SF5 富集的 MLCS 上,导致线粒体分裂和自噬。TM4SF5 与 NPC 细胞内胆固醇转运体 1(NPC1)和游离胆固醇结合,介导溶酶体胆固醇向线粒体输出,导致氧化磷酸化受损,但三羧酸(TCA)循环和 β 氧化作用完好无损。在小鼠模型中,肝细胞Tm4sf5促进了有丝分裂吞噬和胆固醇向线粒体的转运,两者都与肝脏恶性程度呈正相关:我们的研究结果表明,富含 TM4SF5 的 MLCS 通过促进胆固醇输出以进行线粒体重编程来调节葡萄糖分解代谢,这可能与肝细胞癌变同时发生,再现了肝细胞癌代谢的各个方面,即线粒体重编程除支持糖酵解能量外,还支持生物大分子合成。
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引用次数: 0
Enhancing sensitivity of triple-negative breast cancer to DNA-damaging therapy through chemical inhibition of the m6A methyltransferase METTL3 通过化学抑制 m6A 甲基转移酶 METTL3 提高三阴性乳腺癌对 DNA 损伤疗法的敏感性
IF 16.2 1区 医学 Q1 ONCOLOGY Pub Date : 2023-12-15 DOI: 10.1002/cac2.12509
Bianca Cesaro, Alessia Iaiza, Fabio Piscopo, Marco Tarullo, Eleonora Cesari, Dante Rotili, Antonello Mai, Alberto Diana, Michela Londero, Luca Del Giacco, Riccardo Masetti, Alba Di Leone, Chiara Naro, Silvia Masciarelli, Giulia Fontemaggi, Claudio Sette, Francesco Fazi, Alessandro Fatica
<p>Dear Editor,</p><p>N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) is a critical mRNA modification catalyzed by the enzyme methyltransferase-like 3 (METTL3), with implications in RNA metabolism. METTL3 upregulation is associated with cancer progression, metastasis, and drug resistance, making it a potential therapeutic target [<span>1</span>]. The small-molecule METTL3 inhibitor, STM2457, has shown promise in treating acute myeloid leukemia (AML) and has demonstrated good tolerance in mice [<span>2, 3</span>]. However, the specific cancer types where METTL3 inhibitors are most effective remain unknown.</p><p>In breast cancer, <i>METTL3</i> knockdown markedly suppresses proliferation, invasiveness, and metastasis [<span>4</span>]. Therefore, METTL3 inhibition is proposed as a therapeutic approach for breast cancer. Triple-negative breast cancer (TNBC), the most aggressive subtype, lacks targeted therapies, and its primary treatments involve conventional chemotherapy and DNA-damaging agents [<span>5</span>]. Homologous recombination deficiency, such as mutations in the breast cancer gene 1 (<i>BRCA1</i>) and <i>BRCA2</i>, serves as a predictive biomarker for identifying patients who would benefit from genotoxic chemotherapy and poly(ADP-ribose) polymerase (<i>PARP</i>) inhibitors. Notably, METTL3 is recruited to DNA-damaged sites and is crucial for subsequent DNA repair [<span>6, 7</span>]. Consequently, <i>METTL3</i> knockdown reduces DNA repair activity and sensitizes cancer cells to genotoxic drugs [<span>7, 8</span>]. However, while TNBC exhibits elevated METTL3 levels, and its nuclear catalytic activity associates with invasiveness and metastasis [<span>9</span>], it remains uncertain whether METTL3 inhibition enhances chemotherapy response in TNBC.</p><p>Here, we aimed to explore the potential of METTL3 catalytic inhibition by STM2457 as a valuable treatment option for TNBC. Furthermore, we assessed the impact of STM2457 on the sensitivity of TNBC cells and a TNBC patient-derived organoid line to clinical DNA-damaging therapies, like platinum-based chemotherapy and the PARP inhibitor olaparib (Supplementary file of methods).</p><p>STM2457 significantly reduced the proliferation and viability of TNBC cells, including both <i>BRCA1/2</i> wild-type (MDA-MB-231 and MDA-MB-468) and <i>BRCA1</i>-mutated (MDA-MB-436, HCC1395, and HCC1937) cell lines. STM2457 exhibited negligible effects on the proliferation of non-tumoral breast epithelial cells (MCF-10A), with significant reduction observed only at the highest concentration tested (100 μmol/L) (Figure 1A, Supplementary Figure S1A-B). The treatment with 10 μmol/L STM2457 for 48 h decreased the global m<sup>6</sup>A levels in mRNA by approximately 50% in both MDA-MB-231 and MCF-10A cells (Supplementary Figure S1C). Colony formation assays further confirmed the anti-proliferative impact of STM2457 on TNBC cell lines (Figure 1B, Supplementary Figure S2). Moreover, wound healing assays indicated that
(B) 用 5 μmol/L STM2457(STM)处理 MDA-MB-231 和 MCF-10A 细胞系的细胞集落形成试验。(C) 用伤口愈合试验评估经 20 μmol/L STM2457 处理的 MDA-MB-231 细胞的迁移能力;直方图表示迁移百分比的平均值(± SD),n = 3。(D)MDA-MB-231 细胞经 10 μmol/L STM、10 μmol/L 顺铂或两种药物联合处理 72 小时后的 MTT 检测。(F)MDA-MB-231 细胞经 10 μmol/L STM、20 μmol/L olaparib 或两种药物联合处理 72 小时后的 MTT 检测。(H)48 hpf 斑马鱼胚胎与 MDA-MB-231 GFP 阳性细胞直接移植到 PVS。上图是 CHT 区域的代表性荧光立体显微镜图像,其中包含 24 hpi 外渗的 GFP 阳性细胞,显示了异种移植的 4 个离散等级(高、中、低、无)。侧视图,左前方。下图,评估细胞通过 48 hpf 斑马鱼胚胎异种移植的外渗能力。每个条代表至少两个独立实验计算得出的 24 hpi 幼体的平均值%。分析的胚胎总数为 213 个,划分如下:DMSO(n = 28)、STM(n = 45)、顺铂(n = 37)、顺铂 + STM(n = 53)、奥拉帕利(n = 26)、奥拉帕利 + STM(n = 24)。(I)BCO-21 的代表性明视野图像;下图为苏木精-伊红染色。(J)STM2457 对 BCO-21 的细胞毒性作用。将细胞暴露于不同浓度的药物中 5 天,用 Cell Titer Glo 3D 检测法评估细胞活力。(K)STM2457 和卡铂或奥拉帕利对 BCO 生命力的协同作用。将 BCO-21 暴露于次优剂量(5 μmol/L)和最优剂量(10 μmol/L)的 STM、卡铂(10 μmol/L)和奥拉帕利(10 μmol/L)联合治疗 5 天。计算出药物组合与单个药物相比的 CI 值 &lt; 1(表示协同作用),并显示在图表上方。所有结果均以三重复的平均值 ± SEM 表示。*P &lt; 0.05,**P &lt; 0.01,***P &lt; 0.001。缩写:缩写:BCO,乳腺癌类器官;CHT,尾部造血组织;CI,组合指数;DMSO,二甲基亚砜;GFP,绿色荧光蛋白;Hpf,受精后数小时;Hpi,注射后数小时;MTT,3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑;PVS,绒毛膜周围间隙;SD,标准差;SEM,均值标准误差;STM,STM2457。首先,我们研究了 METTL3 抑制与 DNA 损伤药物联合使用的效果。铂类化疗药物和 PARP 抑制剂(如奥拉帕利)对携带 BRCA1/2 基因突变的 TNBC 大多显示出良好的反应[5]。由于 STM2457 下调了参与 DNA 修复途径的基因,我们假设它也可能降低 BRCA1/2 野生型 TNBC 细胞的同源重组(HR)能力。为了验证这一假设,我们研究了抑制 METTL3 对 MDA-MB-231 细胞对顺铂和奥拉帕利反应的影响,MDA-MB-231 细胞是 BRCA1/2 野生型细胞,对 DNA 损伤药物的敏感性较低。用低于半数最大抑制浓度(IC50)的 STM2457、顺铂和奥拉帕利作为单药或联合用药治疗 MDA-MB-231 细胞。与单药治疗相比,STM2457与顺铂或奥拉帕利联合治疗可显著减少MDA-MB-231细胞的增殖并增加细胞凋亡(图1D-G)。值得注意的是,单用 STM2457 就足以诱导 DNA 损伤,H2A 组蛋白家族成员 X(γH2AX)的磷酸化升高就说明了这一点(补充图 S7A-B)。此外,我们还注意到,DNA 损伤后,RecA 相关蛋白 51 (RAD51) 蛋白水平和 RAD51 病灶全面下降(附图 S7C-D)。这些发现与敲除 METTL3 表达的细胞中的发现一致[7, 8]。此外,我们还观察到 DNA 损伤在联合处理后持续大幅增加(补充图 S7A-B)。这些数据共同表明,STM2457能使具有野生型BRCA1/2的HR-proficient TNBC细胞对基因毒性化疗或PARP抑制剂诱导的DNA损伤产生强烈的敏感性。由于STM2457治疗能减少TNBC细胞的迁移,我们进一步探讨了它对癌细胞转移的影响。我们选择了转移性TNBC细胞系MDA-MB-231注射到斑马鱼幼体中,斑马鱼幼体是研究转移的一种成熟的体内异种移植模型[10]。
{"title":"Enhancing sensitivity of triple-negative breast cancer to DNA-damaging therapy through chemical inhibition of the m6A methyltransferase METTL3","authors":"Bianca Cesaro,&nbsp;Alessia Iaiza,&nbsp;Fabio Piscopo,&nbsp;Marco Tarullo,&nbsp;Eleonora Cesari,&nbsp;Dante Rotili,&nbsp;Antonello Mai,&nbsp;Alberto Diana,&nbsp;Michela Londero,&nbsp;Luca Del Giacco,&nbsp;Riccardo Masetti,&nbsp;Alba Di Leone,&nbsp;Chiara Naro,&nbsp;Silvia Masciarelli,&nbsp;Giulia Fontemaggi,&nbsp;Claudio Sette,&nbsp;Francesco Fazi,&nbsp;Alessandro Fatica","doi":"10.1002/cac2.12509","DOIUrl":"10.1002/cac2.12509","url":null,"abstract":"&lt;p&gt;Dear Editor,&lt;/p&gt;&lt;p&gt;N&lt;sup&gt;6&lt;/sup&gt;-methyladenosine (m&lt;sup&gt;6&lt;/sup&gt;A) is a critical mRNA modification catalyzed by the enzyme methyltransferase-like 3 (METTL3), with implications in RNA metabolism. METTL3 upregulation is associated with cancer progression, metastasis, and drug resistance, making it a potential therapeutic target [&lt;span&gt;1&lt;/span&gt;]. The small-molecule METTL3 inhibitor, STM2457, has shown promise in treating acute myeloid leukemia (AML) and has demonstrated good tolerance in mice [&lt;span&gt;2, 3&lt;/span&gt;]. However, the specific cancer types where METTL3 inhibitors are most effective remain unknown.&lt;/p&gt;&lt;p&gt;In breast cancer, &lt;i&gt;METTL3&lt;/i&gt; knockdown markedly suppresses proliferation, invasiveness, and metastasis [&lt;span&gt;4&lt;/span&gt;]. Therefore, METTL3 inhibition is proposed as a therapeutic approach for breast cancer. Triple-negative breast cancer (TNBC), the most aggressive subtype, lacks targeted therapies, and its primary treatments involve conventional chemotherapy and DNA-damaging agents [&lt;span&gt;5&lt;/span&gt;]. Homologous recombination deficiency, such as mutations in the breast cancer gene 1 (&lt;i&gt;BRCA1&lt;/i&gt;) and &lt;i&gt;BRCA2&lt;/i&gt;, serves as a predictive biomarker for identifying patients who would benefit from genotoxic chemotherapy and poly(ADP-ribose) polymerase (&lt;i&gt;PARP&lt;/i&gt;) inhibitors. Notably, METTL3 is recruited to DNA-damaged sites and is crucial for subsequent DNA repair [&lt;span&gt;6, 7&lt;/span&gt;]. Consequently, &lt;i&gt;METTL3&lt;/i&gt; knockdown reduces DNA repair activity and sensitizes cancer cells to genotoxic drugs [&lt;span&gt;7, 8&lt;/span&gt;]. However, while TNBC exhibits elevated METTL3 levels, and its nuclear catalytic activity associates with invasiveness and metastasis [&lt;span&gt;9&lt;/span&gt;], it remains uncertain whether METTL3 inhibition enhances chemotherapy response in TNBC.&lt;/p&gt;&lt;p&gt;Here, we aimed to explore the potential of METTL3 catalytic inhibition by STM2457 as a valuable treatment option for TNBC. Furthermore, we assessed the impact of STM2457 on the sensitivity of TNBC cells and a TNBC patient-derived organoid line to clinical DNA-damaging therapies, like platinum-based chemotherapy and the PARP inhibitor olaparib (Supplementary file of methods).&lt;/p&gt;&lt;p&gt;STM2457 significantly reduced the proliferation and viability of TNBC cells, including both &lt;i&gt;BRCA1/2&lt;/i&gt; wild-type (MDA-MB-231 and MDA-MB-468) and &lt;i&gt;BRCA1&lt;/i&gt;-mutated (MDA-MB-436, HCC1395, and HCC1937) cell lines. STM2457 exhibited negligible effects on the proliferation of non-tumoral breast epithelial cells (MCF-10A), with significant reduction observed only at the highest concentration tested (100 μmol/L) (Figure 1A, Supplementary Figure S1A-B). The treatment with 10 μmol/L STM2457 for 48 h decreased the global m&lt;sup&gt;6&lt;/sup&gt;A levels in mRNA by approximately 50% in both MDA-MB-231 and MCF-10A cells (Supplementary Figure S1C). Colony formation assays further confirmed the anti-proliferative impact of STM2457 on TNBC cell lines (Figure 1B, Supplementary Figure S2). Moreover, wound healing assays indicated that ","PeriodicalId":9495,"journal":{"name":"Cancer Communications","volume":"44 2","pages":"282-286"},"PeriodicalIF":16.2,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cac2.12509","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138741751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cover Image, Volume 43, Issue 12 封面图片,第43卷,第12期
IF 16.2 1区 医学 Q1 ONCOLOGY Pub Date : 2023-12-02 DOI: 10.1002/cac2.12508
Yuting Li, Hanhao Zheng, Yuming Luo, Yan Lin, Mingjie An, Yao Kong, Yue Zhao, Yina Yin, Le Ai, Jian Huang, Changhao Chen

The cover image is based on the Original Article An HGF-dependent positive feedback loop between bladder cancer cells and fibroblasts mediates lymphangiogenesis and lymphatic metastasis by Yuting Li et al., https://doi.org/10.1002/cac2.12470.

封面图片来源于李玉婷等人https://doi.org/10.1002/cac2.12470的文章膀胱癌细胞和成纤维细胞之间hgf依赖的正反馈回路介导淋巴管生成和淋巴转移。
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引用次数: 0
LncRNA-CCAT5-mediated crosstalk between Wnt/β-Catenin and STAT3 signaling suggests novel therapeutic approaches for metastatic gastric cancer with high Wnt activity lncrna - ccat5介导的Wnt/β-Catenin与STAT3信号之间的串扰提示了高Wnt活性转移性胃癌的新治疗方法。
IF 16.2 1区 医学 Q1 ONCOLOGY Pub Date : 2023-11-27 DOI: 10.1002/cac2.12507
Chenchen Liu, Aiwen Shen, Junquan Song, Lei Cheng, Meng Zhang, Yanong Wang, Xiaowen Liu
<div> <section> <h3> Background</h3> <p>Although the constitutively activated Wnt/β-catenin signaling pathway plays vital roles in gastric cancer (GC) progression, few Wnt inhibitors are approved for clinical use. Additionally, the clinical significance of long non-coding RNAs (lncRNAs) in GC intraperitoneal dissemination (IPD) remains elusive. Here, we investigated the function and therapeutic potential of Wnt-transactivated lncRNA, colon cancer-associated transcript 5 (CCAT5), in GC metastasis.</p> </section> <section> <h3> Methods</h3> <p>LncRNA-sequencing assay was performed to document abundance changes of lncRNAs induced by Wnt family member 3A (Wnt3a) and degradation-resistant β-catenin (S33Y mutated) in ascites-derived GC cells with low Wnt activity. Luciferase reporter, Chromatin immunoprecipitation (ChIP)-re-ChIP assays were performed to determine how CCAT5 was transcribed. The clinical significance of CCAT5 was examined in 2 cohorts of GC patients. The biological function of CCAT5 was investigated through gain- and loss-of-function studies. The molecular mechanism was explored through RNA-sequencing, mass spectrometry, and CRISPR/Cas9-knocknout system. The therapeutic potential of CCAT5 was examined through RNAi-based cell xenograft model and patient-derived xenograft (PDX) model of IPD.</p> </section> <section> <h3> Results</h3> <p>We identified a novel Wnt-regulated lncRNA, CCAT5, which was transactivated by the β-catenin/transcription factor 3 (TCF3) complex. CCAT5 was significantly upregulated in GC and predicted poor prognosis. Functional studies confirmed the promotive role of CCAT5 in GC growth and metastasis. Mechanistically, CCAT5 bound to the C-end domain of signal transducer and activator of transcription 3 (STAT3) and blocks Src homology 2 domain-containing protein tyrosine phosphatase 1 (SHP-1)-mediated STAT3<sup>Y705</sup> dephosphorylation, leading to STAT3 nuclear entry and transactivation, thus accelerating GC progression. Furthermore, we demonstrated that both Wnt3a and β-catenin acted as activator of STAT3 signaling pathway, and the interplay between CCAT5 and STAT3 was functionally essential for Wnt-drived STAT3 signaling and tumor evolution. Finally, we revealed in vivo si-CCAT5 selectively attenuated growth and metastasis of Wnt<sup>high</sup> GC, but not Wnt<sup>low</sup> GC. The combination of si-CCAT5 and oxaliplatin displayed obvious synergistic therapeutic effects on Wnt<sup>high</sup> PDX mice.</p> </section> <section> <h3> Conclusions</h3> <p>We identified a novel Wnt-transactivated lncRNA, CCAT5. Our study r
背景:虽然组成性激活的Wnt/β-catenin信号通路在胃癌(GC)的进展中起着至关重要的作用,但很少有Wnt抑制剂被批准用于临床。此外,长链非编码rna (lncRNAs)在GC腹腔播散(IPD)中的临床意义尚不明确。在这里,我们研究了wnt转激活的lncRNA结肠癌相关转录物5 (CCAT5)在胃癌转移中的功能和治疗潜力。方法:采用lncrna测序法,记录Wnt家族成员3A (Wnt3a)和抗降解β-catenin (S33Y突变)在Wnt活性低的腹水源性GC细胞中诱导的lncrna丰度变化。采用荧光素酶报告基因、染色质免疫沉淀(ChIP)-re-ChIP测定CCAT5的转录方式。在两组胃癌患者中检测CCAT5的临床意义。CCAT5的生物学功能通过功能获得和功能丧失研究进行了研究。通过rna测序、质谱分析和CRISPR/ cas9敲除系统探索其分子机制。通过基于rnai的细胞异种移植模型和IPD患者源异种移植(PDX)模型检测CCAT5的治疗潜力。结果:我们发现了一个新的wnt调控lncRNA CCAT5,它被β-catenin/转录因子3 (TCF3)复合物反激活。CCAT5在GC中显著上调,预示预后不良。功能研究证实了CCAT5在胃癌生长和转移中的促进作用。在机制上,CCAT5结合到信号换能器和转录激活子3 (STAT3)的c端结构域,阻断含有Src同源2结构域的蛋白酪氨酸磷酸酶1 (SHP-1)介导的STAT3Y705去磷酸化,导致STAT3进入核并转激活,从而加速GC的进展。此外,我们证明Wnt3a和β-catenin都是STAT3信号通路的激活剂,并且CCAT5和STAT3之间的相互作用对于wnt驱动的STAT3信号传导和肿瘤进化在功能上是必不可少的。最后,我们发现体内si-CCAT5选择性地抑制Wnthigh GC的生长和转移,但对wnlow GC没有作用。si-CCAT5联合奥沙利铂对Wnthigh PDX小鼠有明显的协同治疗作用。结论:我们发现了一种新的wnt转激活lncRNA CCAT5。我们的研究揭示了STAT3信号通过典型Wnt信号调控的机制,以及CCAT5作为关键中介的功能意义。我们提供了lncrna作为逆转GC进展的治疗靶点的概念进展。
{"title":"LncRNA-CCAT5-mediated crosstalk between Wnt/β-Catenin and STAT3 signaling suggests novel therapeutic approaches for metastatic gastric cancer with high Wnt activity","authors":"Chenchen Liu,&nbsp;Aiwen Shen,&nbsp;Junquan Song,&nbsp;Lei Cheng,&nbsp;Meng Zhang,&nbsp;Yanong Wang,&nbsp;Xiaowen Liu","doi":"10.1002/cac2.12507","DOIUrl":"10.1002/cac2.12507","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Background&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Although the constitutively activated Wnt/β-catenin signaling pathway plays vital roles in gastric cancer (GC) progression, few Wnt inhibitors are approved for clinical use. Additionally, the clinical significance of long non-coding RNAs (lncRNAs) in GC intraperitoneal dissemination (IPD) remains elusive. Here, we investigated the function and therapeutic potential of Wnt-transactivated lncRNA, colon cancer-associated transcript 5 (CCAT5), in GC metastasis.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;LncRNA-sequencing assay was performed to document abundance changes of lncRNAs induced by Wnt family member 3A (Wnt3a) and degradation-resistant β-catenin (S33Y mutated) in ascites-derived GC cells with low Wnt activity. Luciferase reporter, Chromatin immunoprecipitation (ChIP)-re-ChIP assays were performed to determine how CCAT5 was transcribed. The clinical significance of CCAT5 was examined in 2 cohorts of GC patients. The biological function of CCAT5 was investigated through gain- and loss-of-function studies. The molecular mechanism was explored through RNA-sequencing, mass spectrometry, and CRISPR/Cas9-knocknout system. The therapeutic potential of CCAT5 was examined through RNAi-based cell xenograft model and patient-derived xenograft (PDX) model of IPD.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;We identified a novel Wnt-regulated lncRNA, CCAT5, which was transactivated by the β-catenin/transcription factor 3 (TCF3) complex. CCAT5 was significantly upregulated in GC and predicted poor prognosis. Functional studies confirmed the promotive role of CCAT5 in GC growth and metastasis. Mechanistically, CCAT5 bound to the C-end domain of signal transducer and activator of transcription 3 (STAT3) and blocks Src homology 2 domain-containing protein tyrosine phosphatase 1 (SHP-1)-mediated STAT3&lt;sup&gt;Y705&lt;/sup&gt; dephosphorylation, leading to STAT3 nuclear entry and transactivation, thus accelerating GC progression. Furthermore, we demonstrated that both Wnt3a and β-catenin acted as activator of STAT3 signaling pathway, and the interplay between CCAT5 and STAT3 was functionally essential for Wnt-drived STAT3 signaling and tumor evolution. Finally, we revealed in vivo si-CCAT5 selectively attenuated growth and metastasis of Wnt&lt;sup&gt;high&lt;/sup&gt; GC, but not Wnt&lt;sup&gt;low&lt;/sup&gt; GC. The combination of si-CCAT5 and oxaliplatin displayed obvious synergistic therapeutic effects on Wnt&lt;sup&gt;high&lt;/sup&gt; PDX mice.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Conclusions&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;We identified a novel Wnt-transactivated lncRNA, CCAT5. Our study r","PeriodicalId":9495,"journal":{"name":"Cancer Communications","volume":"44 1","pages":"76-100"},"PeriodicalIF":16.2,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cac2.12507","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138443897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Single-cell and bulk transcriptomics identifies a tumor-specific CD36+ cancer-associated fibroblast subpopulation in colorectal cancer 单细胞和大量转录组学鉴定结直肠癌中肿瘤特异性CD36+癌症相关成纤维细胞亚群。
IF 16.2 1区 医学 Q1 ONCOLOGY Pub Date : 2023-11-21 DOI: 10.1002/cac2.12506
Jin Wang, Ming-Jia Xi, Qing Lu, Bi-Han Xia, Yu-Zhi Liu, Jin-Lin Yang
<p>Dear Editor,</p><p>Cancer-associated fibroblasts (CAFs) are highly versatile and plastic cells in the tumor microenvironment. They have been identified as actively involved in cancer progression and metastasis through their various roles in remodeling the extracellular matrix, suppressing the immune response and reprogramming tumor metabolism [<span>1</span>]. However, many challenges exist in revealing the functional phenotypes and mechanisms of CAFs in different cancers due to limited understanding of CAF heterogeneity [<span>2</span>]. Recent advances in single-cell transcriptome technology have enabled the identification of distinct CAF subpopulations by using unique gene signatures in multiple tumor types [<span>2</span>]. In this study, we successfully identified a tumor-specific CD36<sup>+</sup> CAF subpopulation in colorectal cancer (CRC), which was found to be correlated with the number of tumor-infiltrated immune cells.</p><p>Primary CAFs and normal fibroblasts (NFs) were isolated from 5 fresh CRC tissues and paired normal colon tissues. Isolation methods and descriptions of other assays are shown in the Supplementary Methods. Immunofluorescence and Western blotting assays showed that the mesenchymal marker Vimentin was expressed in both NFs and CAFs, while alpha smooth muscle actin (αSMA) and fibroblast-specific protein 1 (FSP1) were overexpressed in CAFs (Supplementary Figure S1A-D). To further investigate gene expression profiles in these fibroblasts, primary NFs and CAFs were subjected to RNA sequencing. The results showed that CD36 was significantly upregulated in CAFs (Supplementary Figure S1E), which was further validated by immunofluorescence and Western blotting assays (Supplementary Figure S1F-G). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of differentially expressed genes between NFs and CAFs indicated significant enrichment in the mitogen-activated protein kinase (MAPK) signaling pathway, phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway and receptor-ligand activity (Supplementary Figure S1H-I), suggesting that CAFs may play a critical role in intracellular and extracellular signal transduction.</p><p>Previous studies have demonstrated that CAFs achieve high heterogeneity and plasticity across different cancer types [<span>1, 2</span>]. To further reveal the characteristics of CAFs in CRC, we performed single-cell RNA-sequencing (scRNA-seq) using isolated NFs and CAFs and detected 21,248 NFs and 18,097 CAFs (Figure 1A). A total of 7 clusters were identified in these fibroblasts by using sample integration analysis based on distinct gene expression signatures. Interestingly, we found that Clusters 3, 5, and 7 were mainly distributed in the CAF group, accounting for 34.5%, 7.7%, and 2.3% of total CAFs, respectively (Figure 1B-E). Cluster gene signature analysis showed that CD36 was expressed in CAF-specific subgroups, such as Clusters 3 and 5; inhibin subunit beta A (INHBA) was m
同样,之前的一些研究也表明,CD36+ CAFs 和 INHBA+ CAFs 可分别预测肝细胞癌(HCC)[3] 和胃癌患者[4] 的不良预后。从机制上讲,CD36+ CAFs 可促进 HCC 中免疫抑制微环境的形成 [3]。为了进一步研究 CD36 和 INHBA 是否参与了 CRC 的免疫微环境调控,我们分析了 TCGA-COAD 队列中的基因表达数据,发现 CD36 和 INHBA 的表达与免疫抑制因子和原发肿瘤性免疫细胞标志物的表达呈正相关、如 C-X-C motif趋化因子配体 12(CXCL12)、肿瘤坏死因子配体超家族成员 4(TNFSF4)、T 细胞免疫球蛋白结构域和粘蛋白结构域-3(TIM-3)和 CD163 的表达呈正相关(补充图 S3A-B)。同样,使用 CIBERSORT 方法估计免疫细胞浸润显示,M2 巨噬细胞浸润与 CD36 表达呈显著正相关,CD8+ T 细胞浸润与 INHBA 表达呈显著负相关(补充图 S3C-H)。基于这些发现,我们利用 CRC 组织进行了 mIHC 染色,发现 CD36+ CAF 富集区的 CD8+ T 细胞浸润显著减少(补充图 S4A-B),而 CD36+ CAF 富集区的 CD68+ CD163+ 巨噬细胞浸润增加(补充图 S4C-D)。CD36 是一种在肿瘤细胞[5]、调节性 T 细胞[6]、CD8+ T 细胞[7]和癌症相关巨噬细胞[8]中表达的清道夫受体,可促进肿瘤转移并在不同癌症类型中诱导免疫抑制表型[5-8]。CD36 表达增高的肿瘤细胞具有独特的转移启动能力,CD36+ 转移启动细胞的存在与多种癌症的不良预后有关 [9]。然而,之前的一项研究表明,CD36 通过抑制糖酵解抑制结直肠肿瘤的发生,而且从腺瘤到癌变,CD36 的表达逐渐减少 [10]。在我们的研究中,我们通过多种方法发现 CD36 在 CRC 细胞和肿瘤基质中均有过表达,并揭示了 CD36 在 CRC 中的高表达预示着患者的不良预后。此外,我们还发现 CD36+ CAFs 的存在与 CRC 中 CD8+ T 细胞浸润减少和 CD68+ CD163+ 巨噬细胞浸润增加有关,这表明 CD36+ CAFs 与抑制性免疫表型有关。总之,CD36+ CAFs是CRC的不良预后因素,并与免疫细胞浸润有关。我们的工作强调了鉴定肿瘤特异性 CAF 亚群对了解异质性肿瘤微环境的重要性。本研究获得了华西医院伦理委员会的批准(编号:2021-179)。JW和JLY设计了研究大纲;JW、MJX和BHX进行了实验;JW、QL和YZL分析了数据。JW和JLY撰写了手稿;JW、YZL和JLY对手稿进行了指导和编辑。所有作者阅读并批准了最终手稿。
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引用次数: 0
Multi-omic study to unmask genes involved in prostate cancer development in a multi-case family 多组学研究揭示了多病例家族中前列腺癌发展的基因。
IF 16.2 1区 医学 Q1 ONCOLOGY Pub Date : 2023-11-21 DOI: 10.1002/cac2.12501
Lucia Chica-Redecillas, Sergio Cuenca-Lopez, Eduardo Andres-Leon, Laura Carmen Terron-Camero, Blanca Cano-Gutierrez, Jose Manuel Cozar, Jose Antonio Lorente, Fernando Vazquez-Alonso, Luis Javier Martinez-Gonzalez, Maria Jesus Alvarez-Cubero
<p>Dear Editor,</p><p>Hereditary prostate cancer (PC) comprises 5%-10% of all PC cases. The increased risk of PC in men with a family history of the disease is well known and is commonly caused by germline mutations, leading to clinical guidelines mentioning various genes for identifying high-risk individuals. However, the complex inheritance patterns involving multiple single nucleotide polymorphisms (SNPs) make it a genetically heterogeneous disease, with genetic testing still in its early stages. Current guidelines, such as those from the National Comprehensive Cancer Network (NCCN), are insufficient to identify and stratify all PC patients [<span>1</span>]. To improve testing and screening for familial PC, we report a multi-omic analysis (Supplementary Figures S1-S2) in a PC multi-case family of seven members (two healthy, four PC, and one breast cancer) (Figure 1A, Supplementary Table S1) combining exome, transcriptome and epigenomic analyses (whole-DNA methylation and small-RNA sequencing), offering a unique perspective on the understanding of hereditary PC to date. Each family is a small genetic unit that differs significantly from others with the same pathology but different genetic origins. Therefore, individualized studies may be the key to unravel the heterogeneity of this disease. However, we need to consider that conducting futuremetabolomic analysis would be next steps to reinforce present data, as well as reproducible analysis in other PC families.</p><p>We selected 34 genes based on NCCN (v1.2023) and European Association of Urology (EAU, v2.0) clinical guidelines and literature [<span>2, 3</span>] (Supplementary Table S2). We found 268 variants in 26 of these genes (<i>APC, ATM, AXIN2, BARD1, BMPR1A, BRCA1/2, CDH1, CDK4, CHEK2, DICER1, MLH1, MSH2/3/6, MUTYH, NF1, PMS2, POLD1, POLE, PTEN, RAD51C/D, SMAD4, STK11</i> and <i>TP53</i>), most of which were intronic (91.4%) and/or unreported (84.3%) (Supplementary Figure S3 and Supplementary Table S3). In addition, genome-wide analysis of high-impact variants revealed only four mutations affecting the major isoforms of the <i>ANAPC1</i>, <i>HIBCH</i>, and <i>MOK</i>, but none of these genes have been previously reported in PC (Supplementary Table S4). Interestingly, despite being high-risk cancer patients, the individuals in the present study's family did not show any pathogenic mutations in the genes specified by clinical guidelines. Furthermore, this is added to the growing evidence for the potential of non-coding mutations, both near-exonic and deep-intronic mutations, in carcinogenesis. There is already evidence of how known tumor suppressor genes are affected by intronic mutations [<span>4</span>]. Exome analysis also reported ten identical mutations in three genes, one in <i>AXIN2</i>, two in <i>DICER1</i> and seven in <i>BARD1</i>, in all PC patients (Supplementary Table S3), suggesting that these mutations may be responsible for the development of cancer in this family. Among th
亲爱的编辑,遗传性前列腺癌(PC)占所有 PC 病例的 5%-10%。众所周知,有家族病史的男性罹患前列腺癌的风险会增加,这通常是由种系突变引起的,因此临床指南中提到了用于识别高危人群的各种基因。然而,涉及多个单核苷酸多态性(SNP)的复杂遗传模式使其成为一种遗传异质性疾病,基因检测仍处于早期阶段。美国国家综合癌症网络(NCCN)等机构制定的现行指南不足以对所有 PC 患者进行识别和分层[1]。为了改善家族性 PC 的检测和筛查,我们报告了一个由 7 名成员(2 名健康、4 名 PC 和 1 名乳腺癌)组成的 PC 多病例家族(图 1A,补充表 S1)的多组学分析(补充图 S1-S2),该分析结合了外显子组、转录组和表观基因组分析(全 DNA 甲基化和小 RNA 测序),为迄今为止对遗传性 PC 的了解提供了一个独特的视角。每个家族都是一个小的遗传单元,与病理相同但遗传起源不同的其他家族有显著差异。因此,个体化研究可能是揭示这种疾病异质性的关键。我们根据 NCCN(v1.2023)和欧洲泌尿外科协会(EAU,v2.0)的临床指南和文献[2, 3]选择了 34 个基因(补充表 S2)。我们在其中 26 个基因(APC、ATM、AXIN2、BARD1、BMPR1A、BRCA1/2、CDH1、CDK4、CHEK2、DICER1、MLH1、MSH2/3/6、MUTYH、NF1、PMS2、POLD1、POLE、PTEN、RAD51C/D、SMAD4、STK11 和 TP53)中发现了 268 个变异,其中大部分为内含子(91.4%)和/或未报告(84.3%)(补充图 S3 和补充表 S3)。此外,对高影响变异的全基因组分析显示,只有 4 个突变影响到 ANAPC1、HIBCH 和 MOK 的主要同工酶,但这些基因以前都未在 PC 中报道过(补充表 S4)。有趣的是,尽管是高危癌症患者,本研究中的家族成员并没有出现临床指南中规定的致病基因突变。此外,越来越多的证据表明,非编码突变(包括近外显子突变和深内显子突变)在致癌过程中具有潜在作用。已有证据表明,已知的肿瘤抑制基因会受到内含子突变的影响[4]。外显子组分析还报告了所有 PC 患者中三个基因的十个相同突变,其中一个在 AXIN2,两个在 DICER1,七个在 BARD1(补充表 S3),这表明这些突变可能是该家族癌症发生的原因。在这十个相同的突变中,BARD1 中的三个突变(c.2001+66A&gt;C、c.1811-69T&gt;C 和 c.1811-77A&gt;G)和 AXIN2 中的一个突变(c.-116-1330C&gt;G)在欧洲人群中的频率低于 0.05。接下来,我们主要关注与β-catenin通路相互作用的新型遗传标记--AXIN2和DICER1,但也提到了其他相关数据。AXIN2和APC作为β-catenin破坏复合体的一部分,在Wnt信号通路中发挥着重要作用。AXIN2 和 APC 作为 β-catenin 破坏复合体的一部分,在 Wnt 信号通路中发挥着重要作用。部分或完全丧失这些基因的活性会导致 β-catenin 活性增加,从而导致靶基因异常激活,促进细胞增殖和存活(图 1B)[5]。最近,DICER1也被认为是β-catenin的靶基因。此外,在肝脏肿瘤中,DICER1 的突变与 β-catenin 的突变相关,导致其被激活。在子宫内膜样癌和分化良好的胎儿肺腺癌中也观察到了这两个基因同时发生突变的现象[6]。基于这一科学背景,我们在所研究的癌症患者中发现了相同的β-catenin突变(c.*13-8742G&gt;A)。这种内含子突变出现在该基因的无义介导衰变异构体(CTNNB1-212)中,可能会影响该基因的调控和质量控制,防止转录错误[7]。APC 或 β-catenin 突变导致的 Wnt 信号激活已在多种癌症类型中被观察到,在高达 22% 的阉割耐药 PC 中也被观察到 [8]。转录组研究显示,β-catenin 的靶基因 ALDH1A1 明显过表达(P 值 = 2.816 × 10-5,Log2-fold change (logFC) = 1.638)[9],而β-catenin 的表达与 PC 进展过程中的致癌转化有关(补充表 S5)。据观察,KIFC1、RRM2 和 CYP1B1 的过表达可激活 Wnt 信号通路。
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引用次数: 0
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Cancer Communications
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