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A new technique for inoculation of conifer seedling roots with the laminated root rot pathogen, Phellinus weirii 研究了针叶树层状根腐病菌(Phellinus weirii)接种针叶树幼苗根系的新技术
Pub Date : 1998-12-01 DOI: 10.1080/07060669809500400
R. Sturrock, G. Reynolds
The efficacy of a novel inoculation technique for infecting conifer seedling roots with Phellinus weirii was demonstrated, Inoculum units were prepared from P. weirii-colonized stem segments of red alder (Alnus rubra) and branch segments of Douglas-fir (Pseudotsuga menziesii). In one experiment, potted and outplanted seedlings of nine coniferous species of known susceptibility to P. weirii (Douglas-fir, grand fir, lodgepole pine, noble fir, Sitka spruce, western hemlock, western redcedar, western white pine, and yellow cedar) were inoculated using the novel inoculum units. For all nine species combined, transfer of ectotrophic P. weirii was greater for potted seedlings than for outplanted seedlings (93% vs. 48%). However, rates of endotrophic P. weirii infection were similar for potted and outplanted seedlings (81% and 77% respectively). Results of this experiment parallel reported species susceptibility to P. weirii. In a second experiment, rooted cuttings of English yew and Pacific yew, coniferous speci...
以红桤木(Alnus rubra)茎段和孟氏杉木(pseudosuga menziesii)枝段为接种单位,研究了一种新的接种技术对针叶树幼苗根的感染效果。在一项试验中,采用新型接种单位接种了9种已知易感的针叶植物(道格拉斯杉、大冷杉、黑松、冷杉、锡特卡云杉、西部铁杉、西部红杉、西部白松和黄杉)的盆栽和外植苗。对于所有9个物种的组合,异养威氏沼虾在盆栽苗中的转移比外植苗大(93%比48%)。然而,盆栽和外植苗的内源性威氏假体感染率相似(分别为81%和77%)。本实验结果与已报道的物种对威氏假体的敏感性相一致。在第二个实验中,英国红豆杉和太平洋红豆杉,针叶树品种的根茎插条…
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引用次数: 11
Effects of inoculum density, wetness duration, and temperature on control of Botrytis cinerea by Gliocladium roseum in raspberry 接种量、湿润时间和温度对覆盆子玫瑰胶霉防治葡萄孢病的影响
Pub Date : 1998-12-01 DOI: 10.1080/07060669809500390
Hai Yu, J. Sutton
Control of Botrytis cinerea by Gliocladium roseum was investigated in detached raspberry shoots maintained in high humidity. Sporulation incidence of B. cinerea in leaf disks, stem segments, stamens, and pistils respectively from shoots inoculated with 103, 104. 105, and 106 conidia of the pathogen mL-1 increased from 13 to 100%, 7 to 100%, 38 to 100%, and 6 to 94%. When shoots were challenge-inoculated after 24 h with 104 to 108 conidia of G. roseum mL-1, in all combinations, sporulation of the pathogen was suppressed completely in leaves, and by > 97% in stems when density of the antagonist was 107 conidia mL-1. Sporulation of the pathogen in stamens and pistils was suppressed more effectively when density of the antagonist was proportionately high. G. roseum developed more extensively in leaves and stamens when postinoculation wetness lasted at least 12 to 24 h at 20 to 30°C, and controlled sporulation of B. cinerea more effectively when conditions allowed the antagonist to establish endophytically. Po...
在高湿条件下,研究了玫瑰胶霉对覆盆子离体芽的防治作用。用103、104接种的嫩枝叶片、茎段、雄蕊和雌蕊的产孢率。病原菌mL-1的分生孢子数分别从13 ~ 100%、7 ~ 100%、38 ~ 100%和6 ~ 94%增加到105、106。用104 ~ 108个分生孢子mL-1攻毒24 h后,在所有组合中,病原菌在叶片中的产孢量均被完全抑制,当拮抗剂密度为107个分生孢子mL-1时,病原菌在茎中的产孢量被抑制97%以上。当拮抗剂浓度相应高时,病原菌在雄蕊和雌蕊中的产孢抑制效果较好。当接种后在20 ~ 30℃条件下保持至少12 ~ 24 h的湿润时,玫瑰霉在叶片和雄蕊中的发育更为广泛;当条件允许拮抗剂在内生条件下建立时,玫瑰霉对灰孢杆菌的产孢控制更为有效。阿宝……
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引用次数: 21
CONTROL STRATEGIES FOR STUBBLE-BORNE PATHOGENS OF WHEAT 小麦茬传病原菌的防治策略
Pub Date : 1998-12-01 DOI: 10.1080/07060669809500405
W. Bockus
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引用次数: 29
RACE DIFFERENTIATION IN PYRENOPHORA TRITICI-REPENTIS AND SURVEY OF PHYSIOLOGIC VARIATION IN WESTERN CANADA 加拿大西部小白蛉的种间分化及生理变异调查
Pub Date : 1998-12-01 DOI: 10.1080/07060669809500410
L. Lamari, J. Gilbert, A. Tekauz
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引用次数: 90
Empirical infection period models for tan spot of wheat 小麦褐斑病的经验侵染期模型
Pub Date : 1998-12-01 DOI: 10.1080/07060669809500409
E. Wolf, L. Francl
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引用次数: 4
Plant-parasitic nematodes associated with turfgrass in golf courses in southern Ontario 与安大略省南部高尔夫球场草坪草相关的植物寄生线虫
Pub Date : 1998-12-01 DOI: 10.1080/07060669809500397
Qing Yu, J. Potter, G. Gilby
One hundred thirty-five soil samples, collected from 15 golfcourses throughout southern Ontario in 1995, 1997, and 1998 were analysed for the presence of plant-parasitic nematodes. Criconemoides spp., Helicotylenchus spp., Heterodera spp., Hoplolaimus spp., Meloidogyne spp., Paratylenchus spp., Pratylenchus spp., Tylenchorhynchus spp., and Xiphinema spp. were recorded. The ring nematodes, Criconemoides spp., and the pin nematodes, Paratylenchus spp., were present in all 15 golf courses. The lesion nematodes, Pratylenchus spp., and the spiral nematodes, Helicotylenchus spp., were present in 14 courses. The lance nematodes, Hoplolaimus spp., were present in 13 courses. The stunt nematodes, Tylenchorhynchus spp., were present in 12 golf courses. Four species of the lesion nematode were recorded: Pratylenchus crenatus, P. neglectus, P. fallax, and P. thornei. This is the first report of P. fallax in Ontario, and the first detection of P. thornei on turf in the province.
研究人员分析了1995年、1997年和1998年从安大略省南部的15个高尔夫球场收集的135个土壤样本是否存在植物寄生线虫。记录到蠓属、尾尾蠓属、异蚊属、蚤蚤属、尾尾蠓属、副尾尾蠓属、尾尾蠓属、尾尾蠓属和Xiphinema属。15个高尔夫球场均有环状线虫和副针状线虫的存在。14个疗程中均有病变线虫和螺旋线虫。长枪线虫,Hoplolaimus spp.出现在13个疗程中。在12个高尔夫球场中发现了绝活线虫。记录了4种病变线虫:crenatpratylenchus、p.a nelectus、p.a fallax和p.a thornei。这是安大略省首次报告的费兰假单胞菌,也是该省首次在草坪上检测到索氏假单胞菌。
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引用次数: 17
Discoloration of wheat kernels by Pyrenophora tritici-repentis 小麦籽粒的变色病
Pub Date : 1998-12-01 DOI: 10.1080/07060669809500407
M. R. Fernandez, R. DePauw, J. Clarke, S. Fox
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引用次数: 43
CLONING OF A TWO-COMPONENT SIGNAL TRANSDUCTION SYSTEM OF XANTHOMONAS CAMPESTRIS PV. PHASEOLI VAR.FUSCANS STRAIN BXPF65 油菜黄单胞菌双组分信号转导系统的克隆。Phaseoli变种fuscans菌株bxpf65
Pub Date : 1998-12-01 DOI: 10.1080/07060669809500395
J. W. Chan, S. Maynard, P. Goodwin
A putative two-component signal transduction system was amplified and cloned from the plant pathogenic bacterium Xanthomonas campestris pv. phaseoli var. fuscans isolate BXPF65. The 620 bp amplified fragment was sequenced and analyzed with the BLAST Enhanced Alignment Utility (BEAUTY). BEAUTY analysis indicates that the putative xanthomonad response regulator contains regions which matched to an ATP phosphorylation site, a σ54-factor interaction domain, and the “receiver” domains of various transcriptional regulators of the two-component signal transduction systems. Similar analysis indicates that the putative histidine kinase has homology with conserved “transmitter” domains of sensor proteins in two-component signal transduction systems. RFLP analysis using the putative signal transduction system showed polymorphisms among the strains.
从植物病原菌油菜黄单胞菌(Xanthomonas campestris pv)中扩增并克隆了一个推定的双组分信号转导系统。分离BXPF65。利用BLAST增强比对工具(Enhanced Alignment Utility, BEAUTY)对620 bp扩增片段进行测序和分析。BEAUTY分析表明,假定的黄单胞菌反应调节因子包含与ATP磷酸化位点、σ54因子相互作用结构域和双组分信号转导系统中各种转录调节因子的“受体”结构域相匹配的区域。类似的分析表明,假定的组氨酸激酶与双组分信号转导系统中传感器蛋白的保守“递质”结构域具有同源性。利用假设的信号转导系统进行的RFLP分析显示菌株之间存在多态性。
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引用次数: 2
Genotypes of the late blight pathogen (Phytophthora infestans) in British Columbia and other regions of Canada during 1993-1997 1993-1997年不列颠哥伦比亚省和加拿大其他地区晚疫病病原(疫霉)的基因型
Pub Date : 1998-12-01 DOI: 10.1080/07060669809500393
Z. Punja, H. Förster, I. Cunningham, M. Coffey
Isolates of Phytophthora infestans were collected from British Columbia at various times during 1993-1997 and from eight other provinces in Canada during 1993-1995 and characterized for mating type (either Al or A2), growth response to metalaxyl (either sensitive-MS or insensitive-MI), and random amplified polymorphic DNA (RAPD) patterns. Among the isolates tested, 312 were from diseased potato cultivars, while 14 originated from tomato or hairy nightshade plants with blight symptoms. A composite of RAPD patterns generated by nine primers following PCR amplification was used to identify novel genotypes based on presence of polymorphic bands. The occurrence in Canada of genotypes that corresponded to US I, US 6, US 7, and US 8 was confirmed during this study. The RAPD primers also identified 15 novel genotypes from British Columbia (BC) and 4 from New Brunswick, regions in which both Al and A2 mating types of P. infestans have previously been found and oospore production reported. In 1993, genotypes US 6 a...
1993-1997年在不列颠哥伦比亚省和1993-1995年在加拿大其他8个省收集了疫霉菌分离株,鉴定了其交配型(Al或A2)、对甲螨灵的生长反应(敏感- ms或不敏感- mi)和随机扩增多态性DNA (RAPD)模式。其中312个分离株来自马铃薯病株,14个分离株来自有疫病症状的番茄和茄类植物。利用PCR扩增后9个引物产生的RAPD模式组合,根据多态性条带的存在来鉴定新的基因型。在这项研究中,加拿大出现了与us1、us6、us7和us8对应的基因型。RAPD引物还从不列颠哥伦比亚省(BC)和新不伦瑞克省(New Brunswick)鉴定出15种新的基因型,这些地区以前发现过Al和A2交配型的感染假单胞菌,并报道过产卵孢子。1993年,基因型us6a…
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引用次数: 20
Effects of carbathiin and thiram with propiconazole and aldicarb on grain yield, and on incidence of root rot, leaf blotch, and root lesion nematode in spring wheat carbathiin和thiram与丙环康唑和灭威对春小麦产量及根腐病、叶斑病和根病线虫发病率的影响
Pub Date : 1998-06-01 DOI: 10.1080/07060669809500428
J. Kimpinski, H. Johnston, J. Sanderson
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引用次数: 3
期刊
Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie
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