Anthocyanins from flowers of common lilac (Syringa vulgaris L.) of various color intensities and shades of lilac color from nine samples purchased at the Belgorod market were studied using reverse-phase high-performance liquid chromatography. To determine the structure of anthocyanins, we used the analysis of electronic absorption spectra recorded in the cuvette of a diode array detector and the analysis of mass spectra obtained by electrospray ionization with partial fragmentation. As a result, it was found that in all the studied samples the main component was delphinidin-3-rutinoside (84–90% by peak areas in the chromatogram). The level of cyanidin-3-rutinoside biosynthesis was significantly lower (6–19.6%). Among the minor compounds, delphinidin-3-glucoside and petunidin-3-glucoside were found. Among the unusual compounds, pyranoanthocyanin, built on the basis of delphinidin-3-rutinoside due to condensation with pyruvic acid, was found in a number of studied samples, but the reasons for its appearance have not yet been established. The total content of anthocyanins is low and amounts to 0.020–0.120 g per 100 g of fresh material (depending on the color intensity of the original plant material) in terms of cyanidin-3-glucoside. By drying flowers on a cut branch, air-dried material was obtained containing 0.100–0.300 g per 100 g of anthocyanins.
{"title":"ANTHOCYANINS IN LILAC FLOWERS SYRINGA VULGARIS","authors":"Irina Petrovna Blinova, V. Deyneka, Yaroslava Yur'yevna Salasiina, Yelena Yur'yevna Oleynits, Lyudmila Aleksandrovna Deyneka","doi":"10.14258/jcprm.20230311638","DOIUrl":"https://doi.org/10.14258/jcprm.20230311638","url":null,"abstract":"Anthocyanins from flowers of common lilac (Syringa vulgaris L.) of various color intensities and shades of lilac color from nine samples purchased at the Belgorod market were studied using reverse-phase high-performance liquid chromatography. To determine the structure of anthocyanins, we used the analysis of electronic absorption spectra recorded in the cuvette of a diode array detector and the analysis of mass spectra obtained by electrospray ionization with partial fragmentation. As a result, it was found that in all the studied samples the main component was delphinidin-3-rutinoside (84–90% by peak areas in the chromatogram). The level of cyanidin-3-rutinoside biosynthesis was significantly lower (6–19.6%). Among the minor compounds, delphinidin-3-glucoside and petunidin-3-glucoside were found. Among the unusual compounds, pyranoanthocyanin, built on the basis of delphinidin-3-rutinoside due to condensation with pyruvic acid, was found in a number of studied samples, but the reasons for its appearance have not yet been established. The total content of anthocyanins is low and amounts to 0.020–0.120 g per 100 g of fresh material (depending on the color intensity of the original plant material) in terms of cyanidin-3-glucoside. By drying flowers on a cut branch, air-dried material was obtained containing 0.100–0.300 g per 100 g of anthocyanins.","PeriodicalId":9946,"journal":{"name":"chemistry of plant raw material","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139324072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-02DOI: 10.14258/jcprm.20230313430
B. Shipunov, Vadim Ivanovich Markin
The paper presents the results that continue the cycle of studies on the effect of a high-frequency field on water, which was later used to prepare agar solutions. The strength of agar gel (jelly) was studied depending on the conditions of field exposure to water. It is shown that the strength of the gel in a complex way depends on the frequency of field pretreatment and the time of water exposure after field exposure. The rheological properties were analyzed using the Ostwald-de Ville approximation equation. The dependence of the rheological constant (consistency coefficient) on the frequency of the field exposure and the exposure time after it is analyzed. Dependencies, as observed earlier, are non-systemic broken curves. The rheological properties and strength of the gel prepared in water that has undergone identical field treatment has been compared. The results can be conditionally divided into three groups: synbate change, antibate change and change of dependencies after 9 days of water exposure. It is shown that the strength and viscosity of the agar gel generally decreases. The complex dependences of strength and viscosity on factors affecting water are explained on the basis of the assumption of a complex and multilevel initial structure of water and its change both under the action of an electromagnetic field and over time as a result of thermal motion.
{"title":"MECHANICAL PROPERTIES OF AGAR SOLUTIONS AND GELS PREPARED USING WATER EXPOSED TO ELEC-TROMAGNETIC FIELD","authors":"B. Shipunov, Vadim Ivanovich Markin","doi":"10.14258/jcprm.20230313430","DOIUrl":"https://doi.org/10.14258/jcprm.20230313430","url":null,"abstract":"The paper presents the results that continue the cycle of studies on the effect of a high-frequency field on water, which was later used to prepare agar solutions. The strength of agar gel (jelly) was studied depending on the conditions of field exposure to water. It is shown that the strength of the gel in a complex way depends on the frequency of field pretreatment and the time of water exposure after field exposure. The rheological properties were analyzed using the Ostwald-de Ville approximation equation. The dependence of the rheological constant (consistency coefficient) on the frequency of the field exposure and the exposure time after it is analyzed. Dependencies, as observed earlier, are non-systemic broken curves. The rheological properties and strength of the gel prepared in water that has undergone identical field treatment has been compared. The results can be conditionally divided into three groups: synbate change, antibate change and change of dependencies after 9 days of water exposure. It is shown that the strength and viscosity of the agar gel generally decreases. The complex dependences of strength and viscosity on factors affecting water are explained on the basis of the assumption of a complex and multilevel initial structure of water and its change both under the action of an electromagnetic field and over time as a result of thermal motion.","PeriodicalId":9946,"journal":{"name":"chemistry of plant raw material","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139324465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The chemical composition of low molecular weight organic compounds (NOS) was studied by chromatography-mass spectrometric analysis of Populus nigra L. kidneys. Of the Caspian region and comparative antimicrobial (bactericidal) activity of extractive components with other plants with characteristic antimicrobial properties. The extraction was carried out by percolation in water-alcohol solutions. The study of antimicrobial activity was carried out by the standard CFU method. As a result, 49 compounds were identified, of which 10 remained unidentified. The study of comparative antimicrobial properties of plant extracts showed the complete bactericidal activity of Populus nigra kidneys against opportunistic microorganisms and microflora of air and water, in MIC equal to 0.25 micrograms/ml. Therefore, the NOSES isolated from the kidneys of Populus nigro of the Caspian region have prospects for further study of antioxidant activity for the preparation of biotechnological drugs and pharmaceutical substances.
{"title":"CHEMICAL COMPOSITION AND BIOLOGICAL PROPERTIES OF THE KIDNEYS OF POPULUS NIGRA L. OF THE CASPIAN REGION","authors":"Lyudmila Timofeyevna Sukhenkо, Evgeny Alexandrovich Kurashov, Yuliya Viktorovna Krylova","doi":"10.14258/jcprm.20230312177","DOIUrl":"https://doi.org/10.14258/jcprm.20230312177","url":null,"abstract":"The chemical composition of low molecular weight organic compounds (NOS) was studied by chromatography-mass spectrometric analysis of Populus nigra L. kidneys. Of the Caspian region and comparative antimicrobial (bactericidal) activity of extractive components with other plants with characteristic antimicrobial properties. The extraction was carried out by percolation in water-alcohol solutions. The study of antimicrobial activity was carried out by the standard CFU method. As a result, 49 compounds were identified, of which 10 remained unidentified. The study of comparative antimicrobial properties of plant extracts showed the complete bactericidal activity of Populus nigra kidneys against opportunistic microorganisms and microflora of air and water, in MIC equal to 0.25 micrograms/ml. Therefore, the NOSES isolated from the kidneys of Populus nigro of the Caspian region have prospects for further study of antioxidant activity for the preparation of biotechnological drugs and pharmaceutical substances.","PeriodicalId":9946,"journal":{"name":"chemistry of plant raw material","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139324559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-02DOI: 10.14258/jcprm.20230312035
Aisha Baba gyzy Hasanova
Plants are natural resource with a perfect structure that can meet many needs of people and show diversity since hundreds of years. All over the world and in our country, plants have been used for a long time in the preparation of tea, spices, perfumes, ointments and medicines used in the treatment of diseases. Essential oils arelarge group of biologically active compounds contained in plants. In the article, the process of extracting essential oil from the juniper plant (Juniperus communis L.) at different yields was studied. Juniper fruit 200.47 g; 200.28 g; when 200.54 g, the average amount of essential oil according to wet raw material was determined to be 1.2±1.4%. The composition of the essential oil obtained from juniper fruit, rich in biologically active substances and important for treatment, was studied by physicochemical methods (chromatographic, Ultraviolet (UV) and Infrared (IR) spectroscopic). In the chromatogram of the essential oil, it was determined that there is a peak of monoterpenoids with a tricyclic structure. Absorption bands obtained as a result of spectral analysis ensure the correctness of the composition of the synthesized essential oil. On the basis of medicinal properties of the juniper plant (Juniperus communis L.), the obtained essential oil can be used in medicine and cosmetology in the preparation of ointments and lotions with various ingredients.
植物是一种结构完美的自然资源，可以满足人们的多种需求，千百年来一直呈现出多样性。在世界各地和我国，植物很早就被用来制茶、香料、香水、药膏和治疗疾病的药物。精油是植物中含有的一大类具有生物活性的化合物。本文研究了不同产量的杜松精油提取过程。从 200.47 克、200.28 克和 200.54 克的杜松果实中提取的精油平均含量为 1.2±1.4%。从杜松果实中提取的精油富含生物活性物质，对治疗具有重要意义，我们通过理化方法（色谱法、紫外线（UV）和红外线（IR）光谱法）对其成分进行了研究。在精油的色谱图中，可以确定有一个三环结构的单萜峰。光谱分析得出的吸收带确保了合成精油成分的正确性。根据杜松植物（Juniperus communis L.）的药用特性，所获得的精油可用于医药和美容领域，与各种成分一起制备软膏和乳液。
{"title":"STUDY OF THE ESSENTIAL OIL OBTAINED FROM JUNIPER PLANT BY PHYSICAL-CHEMICAL METHODS","authors":"Aisha Baba gyzy Hasanova","doi":"10.14258/jcprm.20230312035","DOIUrl":"https://doi.org/10.14258/jcprm.20230312035","url":null,"abstract":"Plants are natural resource with a perfect structure that can meet many needs of people and show diversity since hundreds of years. All over the world and in our country, plants have been used for a long time in the preparation of tea, spices, perfumes, ointments and medicines used in the treatment of diseases. Essential oils arelarge group of biologically active compounds contained in plants. In the article, the process of extracting essential oil from the juniper plant (Juniperus communis L.) at different yields was studied. Juniper fruit 200.47 g; 200.28 g; when 200.54 g, the average amount of essential oil according to wet raw material was determined to be 1.2±1.4%. The composition of the essential oil obtained from juniper fruit, rich in biologically active substances and important for treatment, was studied by physicochemical methods (chromatographic, Ultraviolet (UV) and Infrared (IR) spectroscopic). In the chromatogram of the essential oil, it was determined that there is a peak of monoterpenoids with a tricyclic structure. Absorption bands obtained as a result of spectral analysis ensure the correctness of the composition of the synthesized essential oil. On the basis of medicinal properties of the juniper plant (Juniperus communis L.), the obtained essential oil can be used in medicine and cosmetology in the preparation of ointments and lotions with various ingredients.","PeriodicalId":9946,"journal":{"name":"chemistry of plant raw material","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139324649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-02DOI: 10.14258/jcprm.20230311781
Olga Vladimirovna Feofilaktova, Vladislav Lvovich Feigelman, Said Abdellatif Said Aboushanab, Elena Geranovna Kovaleva
Ultrasonic homogenization is a promising method of emulsion formation. Linseed oil containing polyunsaturated fatty acids in the optimal ratio was used as a control sample and the fat base of the emulsions. The effect of ultrasonic treatment can lead to a change in the percentage of fatty acids due to the acceleration and initiation of several chemical reactions. To assess the feasibility of using ultrasound in food production, the fatty acid composition of natural linseed oil and emulsions obtained from it, treated by ultrasound with different durations were studied. The study monitored the formation of radicals since increasing temperature and pressure during the collapse of cavitation bubbles initiates the formation of free hydrogen H- and hydroxyl OH- radicals within and next to them. These may initiate the oxidation of bioorganic compounds in the food product. The research results show that emulsions exposure to the ultrasound at a frequency of 20 kHz for 10, 20 and 30 minutes are capable to maintain the ratio of fatty acids while obtaining a homogeneous, finely dispersed and stable food emulsion. Deviations in the values characterizing the fatty acids content in the emulsions treated by ultrasound for 10, 20 and 30 minutes as compared with the control sample – linseed oil are insignificant (0.02±0.015% to 0.83±0.015). Using the method of electron paramagnetic resonance, the absence of free radicals was established both in the control sample – flax oil, and in emulsions obtained on its basis, homogenized using ultrasonic exposure.
{"title":"ULTRASONIC CAVITATION EFFECT ON THE FATTY ACID COMPOSITION OF LINSEED OIL-BASED EMULSIONS","authors":"Olga Vladimirovna Feofilaktova, Vladislav Lvovich Feigelman, Said Abdellatif Said Aboushanab, Elena Geranovna Kovaleva","doi":"10.14258/jcprm.20230311781","DOIUrl":"https://doi.org/10.14258/jcprm.20230311781","url":null,"abstract":"Ultrasonic homogenization is a promising method of emulsion formation. Linseed oil containing polyunsaturated fatty acids in the optimal ratio was used as a control sample and the fat base of the emulsions. The effect of ultrasonic treatment can lead to a change in the percentage of fatty acids due to the acceleration and initiation of several chemical reactions. To assess the feasibility of using ultrasound in food production, the fatty acid composition of natural linseed oil and emulsions obtained from it, treated by ultrasound with different durations were studied. The study monitored the formation of radicals since increasing temperature and pressure during the collapse of cavitation bubbles initiates the formation of free hydrogen H- and hydroxyl OH- radicals within and next to them. These may initiate the oxidation of bioorganic compounds in the food product. The research results show that emulsions exposure to the ultrasound at a frequency of 20 kHz for 10, 20 and 30 minutes are capable to maintain the ratio of fatty acids while obtaining a homogeneous, finely dispersed and stable food emulsion. Deviations in the values characterizing the fatty acids content in the emulsions treated by ultrasound for 10, 20 and 30 minutes as compared with the control sample – linseed oil are insignificant (0.02±0.015% to 0.83±0.015). Using the method of electron paramagnetic resonance, the absence of free radicals was established both in the control sample – flax oil, and in emulsions obtained on its basis, homogenized using ultrasonic exposure.","PeriodicalId":9946,"journal":{"name":"chemistry of plant raw material","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139324715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-02DOI: 10.14258/jcprm.20230311724
Khilola Tozhimurodovna Agzamkhuzhaeva, Yulia Igorevna Oshchepkova, S. I. Salikhov
The aim of this work is to study the composition of ribosome-inactivating proteins from the bark and berries of the black elderberry Sambucus nigra L., growing on the territory of the Republic of Uzbekistan. The composition of ribosome-inactivating proteins in the bark and berries of black elder Sambucus nigra L., collected in the Botanical Garden of the Republic of Uzbekistan, was studied. According to HPLC-MS/MS sequencing, the bark sample revealed the presence of 9 RIP-2. Based on the obtained amino acid sequences using the CLUSTAL O (1.2.4) program, the amino acid sequence of the identified type 2 RIPs was compared and similar and conserved residues were identified, which will further help identify new RIPs. In the course of the studies, the tryptic peptide LSLVVLQMVSEAAR containing the RIP active site motif (EAAR) was identified with a high degree of probability. Mass spectral data on this fragment will allow further identification of other RIP-1/2 from other samples of natural origin. 2 RIPs were isolated from berries, one of which is in a reduced form containing one chain. Establishing the amino acid sequence in the isolated RIP from black elderberries with a molecular mass of 62337 Da confirmed our hypothesis of identification by the tryptic peptide containing the RIP active center (EAAR).
{"title":"RIBOSOME-INACTIVATED PROTEINS (RIPS) OF THE BLACK ELDER SAMBUCUS NIGRA","authors":"Khilola Tozhimurodovna Agzamkhuzhaeva, Yulia Igorevna Oshchepkova, S. I. Salikhov","doi":"10.14258/jcprm.20230311724","DOIUrl":"https://doi.org/10.14258/jcprm.20230311724","url":null,"abstract":"The aim of this work is to study the composition of ribosome-inactivating proteins from the bark and berries of the black elderberry Sambucus nigra L., growing on the territory of the Republic of Uzbekistan. The composition of ribosome-inactivating proteins in the bark and berries of black elder Sambucus nigra L., collected in the Botanical Garden of the Republic of Uzbekistan, was studied. According to HPLC-MS/MS sequencing, the bark sample revealed the presence of 9 RIP-2. Based on the obtained amino acid sequences using the CLUSTAL O (1.2.4) program, the amino acid sequence of the identified type 2 RIPs was compared and similar and conserved residues were identified, which will further help identify new RIPs. In the course of the studies, the tryptic peptide LSLVVLQMVSEAAR containing the RIP active site motif (EAAR) was identified with a high degree of probability. Mass spectral data on this fragment will allow further identification of other RIP-1/2 from other samples of natural origin. 2 RIPs were isolated from berries, one of which is in a reduced form containing one chain. Establishing the amino acid sequence in the isolated RIP from black elderberries with a molecular mass of 62337 Da confirmed our hypothesis of identification by the tryptic peptide containing the RIP active center (EAAR).","PeriodicalId":9946,"journal":{"name":"chemistry of plant raw material","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139324819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The development of analytical methods for the standardization of a substance, as well as dosage forms based on it, is an actual task in the production of pharmacological preparations. In connection with the increasing requirements for the quality of active pharmaceutical ingredients, it is advisable to include modern chromatographic methods (HPLC and GLC) in them. In addition, the prospect of introducing drugs into medical practice determines the need to develop highly sensitive methods for determining active substances in substances for pharmacokinetic studies. The aerial part of Arundo donax L. is the main raw material for the industrial production of the drug substance Donaxin hydrochloride. Donaxin is an alkaloid of the indole group and has the chemical structure of 3-(dimethylaminomethyl) indole. Previously, for the quantitative determination of donaxin hydrochloride in the substance of the drug of the same name, the titrometry method was used, according to the results of which the content of the active substance was at least 98.0%. The aim of this study was to develop and validate a new modern technique that exceeds the titration method in selectivity, reproducibility and precision. We have developed and validated a new method for the quantitative determination of Donaxin hydrochloride using high performance liquid chromatography (HPLC). This technique is simple and well reproduced. The developed method was validated according to the following indicators: specificity, linearity, precision, limit of detection and limit of quantitation. A comparative evaluation of the developed method for the quantitative determination of Donaxin hydrochloride in the substance by HPLC with the method included in the temporary pharmacopoeia article (titrimetric) was carried out in terms of the Fisher criterion.
在药物制剂的生产过程中，制定分析方法以实现物质和剂型的标准化是一项实际工作。随着对活性药物成分质量要求的不断提高，最好将现代色谱法（高效液相色谱法和高效液相色谱法）纳入其中。此外，将药物引入医疗实践的前景决定了有必要开发高灵敏度的方法，以确定药物动力学研究物质中的活性物质。Arundo donax L. 的气生部分是工业化生产药物物质盐酸多纳新的主要原料。Donaxin 是一种吲哚类生物碱，化学结构为 3-（二甲基氨基甲基）吲哚。此前，曾使用滴定法对同名药物物质中的盐酸多那新进行定量测定，根据测定结果，活性物质的含量至少为 98.0%。这项研究的目的是开发和验证一种新的现代技术，它在选择性、重现性和精确性方面都超过了滴定法。我们开发并验证了一种使用高效液相色谱法（HPLC）定量测定盐酸多那新的新方法。该技术操作简单，重现性好。所开发的方法根据以下指标进行了验证：特异性、线性、精密度、检出限和定量限。根据费舍尔标准，对所开发的用高效液相色谱法定量测定物质中盐酸多那新的方法与临时药典条款中的方法（滴定法）进行了比较评价。
{"title":"QUANTITATIVE DETERMINATION OF THE ALKALOID DONAXIN IN THE SUBSTANCE DONAXINE HYDROCHLORIDE","authors":"Dilnoza Karimberzhievna Mutalova, Ruzali Anvarovich Botirov, Alimdzhan Zairovich Sadikov, S. Sagdullaev","doi":"10.14258/jcprm.20230311441","DOIUrl":"https://doi.org/10.14258/jcprm.20230311441","url":null,"abstract":"The development of analytical methods for the standardization of a substance, as well as dosage forms based on it, is an actual task in the production of pharmacological preparations. In connection with the increasing requirements for the quality of active pharmaceutical ingredients, it is advisable to include modern chromatographic methods (HPLC and GLC) in them. In addition, the prospect of introducing drugs into medical practice determines the need to develop highly sensitive methods for determining active substances in substances for pharmacokinetic studies. The aerial part of Arundo donax L. is the main raw material for the industrial production of the drug substance Donaxin hydrochloride. Donaxin is an alkaloid of the indole group and has the chemical structure of 3-(dimethylaminomethyl) indole. Previously, for the quantitative determination of donaxin hydrochloride in the substance of the drug of the same name, the titrometry method was used, according to the results of which the content of the active substance was at least 98.0%. The aim of this study was to develop and validate a new modern technique that exceeds the titration method in selectivity, reproducibility and precision. We have developed and validated a new method for the quantitative determination of Donaxin hydrochloride using high performance liquid chromatography (HPLC). This technique is simple and well reproduced. The developed method was validated according to the following indicators: specificity, linearity, precision, limit of detection and limit of quantitation. A comparative evaluation of the developed method for the quantitative determination of Donaxin hydrochloride in the substance by HPLC with the method included in the temporary pharmacopoeia article (titrimetric) was carried out in terms of the Fisher criterion.","PeriodicalId":9946,"journal":{"name":"chemistry of plant raw material","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139324201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-02DOI: 10.14258/jcprm.20230312428
M. Karabayeva, Saodat Rakhmatzhanovna Mirsalimova, D. Salikhanova, N. N. Ubaydullayeva
Currently, the world of environmental protection through the disposal of industrial waste and their rational processing is one of the main problems at the global level, aimed at improving the well-being of the population. However, the rapid development of industrial enterprises is accompanied by the formation of a significant amount of various wastes, including wastewater. Wastewater treatment from various pollutants is important from an environmental and economic point of view. At the same time, the accumulation of plant waste is an unimportant problem. One of the solutions to this problem is the production of environmentally friendly adsorbents based on plant materials. Recent years The recovery of biomass and waste biomass in the last decade has attracted the attention of an increasing number of scientists. Activated carbon (AC), used to remove organic micro-pollutants in the world's wastewater treatment plants, is usually produced from non-renewable resources such as peat, lignite, coal, anthracite, wood materials, waste paper, leather industry and animal products that needs to be transported over long distances. The use of biomass as feedstock can be beneficial in terms of sustainability. The review is devoted to the analysis of publications in this field in recent years. The main areas of application of adsorbents based on pine cones are considered. Examples of the implementation of the production of an adsorbent based on plant wastes and their use for water purification from various chemicals are given.
{"title":"ADSORPTION WATER TREATMENT WITH ADSORBENTS BASED ON VEGETABLE RAW MATERIALS","authors":"M. Karabayeva, Saodat Rakhmatzhanovna Mirsalimova, D. Salikhanova, N. N. Ubaydullayeva","doi":"10.14258/jcprm.20230312428","DOIUrl":"https://doi.org/10.14258/jcprm.20230312428","url":null,"abstract":"Currently, the world of environmental protection through the disposal of industrial waste and their rational processing is one of the main problems at the global level, aimed at improving the well-being of the population. However, the rapid development of industrial enterprises is accompanied by the formation of a significant amount of various wastes, including wastewater. Wastewater treatment from various pollutants is important from an environmental and economic point of view. At the same time, the accumulation of plant waste is an unimportant problem. One of the solutions to this problem is the production of environmentally friendly adsorbents based on plant materials. Recent years The recovery of biomass and waste biomass in the last decade has attracted the attention of an increasing number of scientists. Activated carbon (AC), used to remove organic micro-pollutants in the world's wastewater treatment plants, is usually produced from non-renewable resources such as peat, lignite, coal, anthracite, wood materials, waste paper, leather industry and animal products that needs to be transported over long distances. The use of biomass as feedstock can be beneficial in terms of sustainability. The review is devoted to the analysis of publications in this field in recent years. The main areas of application of adsorbents based on pine cones are considered. Examples of the implementation of the production of an adsorbent based on plant wastes and their use for water purification from various chemicals are given.","PeriodicalId":9946,"journal":{"name":"chemistry of plant raw material","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139324378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-02DOI: 10.14258/jcprm.20230412787
O. R. Akhmedov, S. Shomurotov, Anastasiya Vyacheslavovna Sidorenko, A. Turaev
Pectin polysaccharides, due to the presence of reactive carboxyl functional groups in the structure, have a high complexing ability. This expands the scope of their practical application for the development of new biologically active substances. In this work, we propose the preparation of complex compounds based on sodium pectate and guanidine hydrochloride. By varying the molar amount of guanidine in relation to the acid groups of the polysaccharide matrix, samples were obtained that differ in the degree of substitution, the content of the amino derivative of the compound, and the inherent viscosity. The optimal ratio of the starting compounds was determined, at which the limiting amount of guanidine is bound to -COO- groups of pectin macromolecules. The structure of the resulting complexes was studied by FTIR spectroscopy. It has been established that the interaction of guanidine with polysaccharide carboxyls is accompanied by a change in the intensity of the absorption bands corresponding to the ionized acid group. Also, the binding of guanidine to the polysaccharide matrix through ionic bonds has been proven by carrying out the hydrolytic cleavage of the complex compound in an acidic medium. It is shown that with an increase in the hydrolysis time from 3 to 24 h, a gradual decrease in the initial degree of substitution of the complex compound is observed. The antimicrobial properties of polymer complexes with different characteristics against Staphylococcus aureus and Escherichia coli were studied.
{"title":"OBTAINING AND INVESTIGATION OF THE BIOLOGICAL ACTIVITY OF GUANIDINE COMPLEXES WITH PECTIN POLYSACCHARIDES","authors":"O. R. Akhmedov, S. Shomurotov, Anastasiya Vyacheslavovna Sidorenko, A. Turaev","doi":"10.14258/jcprm.20230412787","DOIUrl":"https://doi.org/10.14258/jcprm.20230412787","url":null,"abstract":"Pectin polysaccharides, due to the presence of reactive carboxyl functional groups in the structure, have a high complexing ability. This expands the scope of their practical application for the development of new biologically active substances. In this work, we propose the preparation of complex compounds based on sodium pectate and guanidine hydrochloride. By varying the molar amount of guanidine in relation to the acid groups of the polysaccharide matrix, samples were obtained that differ in the degree of substitution, the content of the amino derivative of the compound, and the inherent viscosity. The optimal ratio of the starting compounds was determined, at which the limiting amount of guanidine is bound to -COO- groups of pectin macromolecules. The structure of the resulting complexes was studied by FTIR spectroscopy. It has been established that the interaction of guanidine with polysaccharide carboxyls is accompanied by a change in the intensity of the absorption bands corresponding to the ionized acid group. Also, the binding of guanidine to the polysaccharide matrix through ionic bonds has been proven by carrying out the hydrolytic cleavage of the complex compound in an acidic medium. It is shown that with an increase in the hydrolysis time from 3 to 24 h, a gradual decrease in the initial degree of substitution of the complex compound is observed. The antimicrobial properties of polymer complexes with different characteristics against Staphylococcus aureus and Escherichia coli were studied.","PeriodicalId":9946,"journal":{"name":"chemistry of plant raw material","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139324700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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