Pub Date : 2021-09-01DOI: 10.12982/cmujns.2021.090
Piyawadee Rugsanit, P. Attasart, A. Udomkit, W. Tirasophon
Abstract Eukaryotic cells have mechanisms to cope with stress in endoplasmic reticulum (ER) called unfolded protein response (UPR). In this study, we characterized putative X-box DNA binding protein1 (XBP1) and Binding protein (BiP) cDNAs from black tiger shrimp (Peneaus monodon). When the shrimp were infected with Yellow head virus (YHV), the levels of XBP1 and BiP mRNA transcripts were elevated approximately 8 and 55 folds, respectively. In normal shrimp, the putative XBP1 (XBP1u) was predicted to encode a protein with 283 amino acid residues. When shrimp were infected with YHV, portion of cDNA with 17 nucleotides intron elimination (XBP1s) was observed. The elimination caused alteration of its translational frame. The predicted protein from this is 469 amino acids in length with total change in its amino acid sequence downstream of the intron. Functional analysis of these XBP1 proteins in mammalian cells clearly showed that overexpression of P. monodon XBP1s was capable of activating the transcription rate of mammalian UPR responsive genes (BiP, ERdj4 and P58IPK). Finally, the impact of XBP1 and BiP on YHV multiplication in black tiger shrimp was investigated by RNAi approach. Knocking down either XBP1 or BiP expression efficiently inhibited YHV replication. Therefore, these two components in the UPR pathway may be an interesting target for anti YHV development in the future. Keywords: Endoplasmic reticulum, siRNA, Stress-inducible genes, Transcriptional regulation
{"title":"Characterization of X- box binding protein 1(XBP1) and BiP functions and investigation their roles in YHV replication in P. monodon","authors":"Piyawadee Rugsanit, P. Attasart, A. Udomkit, W. Tirasophon","doi":"10.12982/cmujns.2021.090","DOIUrl":"https://doi.org/10.12982/cmujns.2021.090","url":null,"abstract":"Abstract Eukaryotic cells have mechanisms to cope with stress in endoplasmic reticulum (ER) called unfolded protein response (UPR). In this study, we characterized putative X-box DNA binding protein1 (XBP1) and Binding protein (BiP) cDNAs from black tiger shrimp (Peneaus monodon). When the shrimp were infected with Yellow head virus (YHV), the levels of XBP1 and BiP mRNA transcripts were elevated approximately 8 and 55 folds, respectively. In normal shrimp, the putative XBP1 (XBP1u) was predicted to encode a protein with 283 amino acid residues. When shrimp were infected with YHV, portion of cDNA with 17 nucleotides intron elimination (XBP1s) was observed. The elimination caused alteration of its translational frame. The predicted protein from this is 469 amino acids in length with total change in its amino acid sequence downstream of the intron. Functional analysis of these XBP1 proteins in mammalian cells clearly showed that overexpression of P. monodon XBP1s was capable of activating the transcription rate of mammalian UPR responsive genes (BiP, ERdj4 and P58IPK). Finally, the impact of XBP1 and BiP on YHV multiplication in black tiger shrimp was investigated by RNAi approach. Knocking down either XBP1 or BiP expression efficiently inhibited YHV replication. Therefore, these two components in the UPR pathway may be an interesting target for anti YHV development in the future. Keywords: Endoplasmic reticulum, siRNA, Stress-inducible genes, Transcriptional regulation","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47799015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.12982/cmujns.2021.072
Irfan Saleem, Z. Ali, A. Hussain, S. Hafeez, G. Joshua
Abstract Almost 30% of the global population is obese and overweight. In Pakistan, the latest research reveals that 28% of peoples are overweight and 16% obese. However, scarce data is available to report a relationship between obesity and pulmonary health in workers. For this reason, the current study aimed to study dynamics of pulmonary function parameters based on body mass index (BMI) categories, through a spirometer device, among wet-blue leather tannery workers. A total of 116 male tannery workers was selected as a studied population. Workers excluded who had age < 20 years, ever-smokers; respiratory disorders, cardiac illness, diabetes, and hypertension. The study was conducted between September to December 2019. Age, height, weight, and BMI were noted. The following BMI categories were used: normal-weight (18.5-24.9), overweight (25.0-29.9), and obese (≥ 30.0). The Pearson correlation test was run to determine was any linear correlation between pulmonary function parameters and BMI categories. Age, weight and BMI showed statistical differences in all BMI categories except height (P= 0.058). In obese and overweight workers, lower mean values of the pulmonary function parameters were observed compared to normal weight workers, respectively. A significant a significant linear correlation between FEV1 %, FVC % and BMI categories except for FEV1/FVC. It was concluded that obese and overweight workers might have reduced pulmonary function parameters. Future studies among tannery workers are highly recommended to find a correlation between BMI categories and pulmonary function with dust exposure. Keywords: Body mass index, Lung, Obesity, Overweight, Pakistan
{"title":"Dynamics of Pulmonary Functions based on Body Mass Index Categories in Wet-Blue Leather Tannery Workers","authors":"Irfan Saleem, Z. Ali, A. Hussain, S. Hafeez, G. Joshua","doi":"10.12982/cmujns.2021.072","DOIUrl":"https://doi.org/10.12982/cmujns.2021.072","url":null,"abstract":"Abstract Almost 30% of the global population is obese and overweight. In Pakistan, the latest research reveals that 28% of peoples are overweight and 16% obese. However, scarce data is available to report a relationship between obesity and pulmonary health in workers. For this reason, the current study aimed to study dynamics of pulmonary function parameters based on body mass index (BMI) categories, through a spirometer device, among wet-blue leather tannery workers. A total of 116 male tannery workers was selected as a studied population. Workers excluded who had age < 20 years, ever-smokers; respiratory disorders, cardiac illness, diabetes, and hypertension. The study was conducted between September to December 2019. Age, height, weight, and BMI were noted. The following BMI categories were used: normal-weight (18.5-24.9), overweight (25.0-29.9), and obese (≥ 30.0). The Pearson correlation test was run to determine was any linear correlation between pulmonary function parameters and BMI categories. Age, weight and BMI showed statistical differences in all BMI categories except height (P= 0.058). In obese and overweight workers, lower mean values of the pulmonary function parameters were observed compared to normal weight workers, respectively. A significant a significant linear correlation between FEV1 %, FVC % and BMI categories except for FEV1/FVC. It was concluded that obese and overweight workers might have reduced pulmonary function parameters. Future studies among tannery workers are highly recommended to find a correlation between BMI categories and pulmonary function with dust exposure. Keywords: Body mass index, Lung, Obesity, Overweight, Pakistan","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45323144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.12982/cmujns.2021.084
D. R. Putri, N. Ismail, R. Idroes, S. Rizal, S. Nur, M. Nanda
Abstract Bur Ni Geureudong is one of geothermal areas that potentially to be developed for geothermal power plant in Aceh Province, Indonesia. Prior to the development, detail investigation based on geological, geophysical and geochemical methods are needed for estimating its potential. However, this site is located in a mountainous area with dense forests that are difficult to reach and research of geothermal exploration in site is still very poor considering its promising potential. So that the use of remote sensing method is very suitable to be done to investigate geothermal potential in these remote areas. For reconnaissance survey, Land Surface Temperature (LST) mapping using Landsat 8 OLI/TIRS (Operational Land Imager/Thermal Infrared Sensor) image data was conducted to investigate the geothermal potential in the area. Radiometric correction, Normalized Difference Vegetation Index (NDVI) mapping and emissivity calculations were performed to obtain the LST map. Results show temperatures in the area ranged 17⁰C to 40⁰C, the area with high surface temperatures are caused by geothermal activities. NDVI map also shows an agreement with the high surface temperature region and they are mostly indicated by occurrence of vegetation stress. Keywords: Bur Ni Geureudong geothermal field, Landsat 8 OLI/TIRS., land surface temperature, Thermal remote sensing
摘要:Bur Ni Geureudong是印度尼西亚亚齐省地热发电潜力开发地区之一。在开发之前，需要通过地质、地球物理和地球化学方法对其进行详细调查，以估计其潜力。然而，该场地地处森林茂密的山区，交通不便，地热勘探潜力巨大，但目前该场地的地热勘探研究还很薄弱。因此，利用遥感方法对这些偏远地区进行地热潜力调查是非常合适的。利用Landsat 8 OLI/TIRS (Operational Land Imager/Thermal Infrared Sensor)影像数据进行地表温度(LST)测图，调查该区地热潜力。通过辐射校正、归一化植被指数(NDVI)作图和发射率计算得到地表温度图。结果显示该地区的温度范围为17⁰C到40⁰C，该地区的高地表温度是由地热活动引起的。NDVI图也与高地温区一致，它们主要由植被胁迫的发生来指示。关键词:布尼热柔洞地热田;Landsat 8 OLI/TIRS;，地表温度，热遥感
{"title":"Analysis of Land Surface Temperature (LST) in Bur Ni Geureudong Geothermal Field, Aceh, Indonesia Using Landsat 8 OLI / TIRS Images","authors":"D. R. Putri, N. Ismail, R. Idroes, S. Rizal, S. Nur, M. Nanda","doi":"10.12982/cmujns.2021.084","DOIUrl":"https://doi.org/10.12982/cmujns.2021.084","url":null,"abstract":"Abstract Bur Ni Geureudong is one of geothermal areas that potentially to be developed for geothermal power plant in Aceh Province, Indonesia. Prior to the development, detail investigation based on geological, geophysical and geochemical methods are needed for estimating its potential. However, this site is located in a mountainous area with dense forests that are difficult to reach and research of geothermal exploration in site is still very poor considering its promising potential. So that the use of remote sensing method is very suitable to be done to investigate geothermal potential in these remote areas. For reconnaissance survey, Land Surface Temperature (LST) mapping using Landsat 8 OLI/TIRS (Operational Land Imager/Thermal Infrared Sensor) image data was conducted to investigate the geothermal potential in the area. Radiometric correction, Normalized Difference Vegetation Index (NDVI) mapping and emissivity calculations were performed to obtain the LST map. Results show temperatures in the area ranged 17⁰C to 40⁰C, the area with high surface temperatures are caused by geothermal activities. NDVI map also shows an agreement with the high surface temperature region and they are mostly indicated by occurrence of vegetation stress. Keywords: Bur Ni Geureudong geothermal field, Landsat 8 OLI/TIRS., land surface temperature, Thermal remote sensing","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43380109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.12982/cmujns.2021.075
Manutsawan Manokieng, A. Jampeetong
Abstract The effects of supplemental cations on growth, nitrogen, and mineral accumulation were assessed in Canna indica L. Similar sized 45 days-old plants were grown on a nutrient solution modified from Hoagland and Arnon (1950). The different cations were added to generate 6 treatments (n=4): (i) control (no cation added), (ii) 2.5 mM K+, (iii) 2.5 mM Ca2+, (iv) 75 mM Na+, (v) 1.25 mM K+ + 1.25 mM Ca2+ and (vi) 2.5 mM Ca2+ + 75 mM Na+, respectively. An experiment was carried out in the greenhouse for 49 days. The study found that supplemental K+ and K++ Ca2+ increased plant growth and total biomass. The highest SER was found in plants receiving supplemental K+. In contrast, SERs, leaf areas, and total biomass decreased in Na+ or Na++Ca2+ supplemented plants. The accumulated NO3- concentration (at the whole plant level) was also highest in the plants with supplemental K+ and K++Ca2+. The total nitrogen accumulation was higher in the K+, Ca2+, and K++Ca2+ supplemented plants than in the control plants. The results suggest that supplemental cations particularly K+ can enhance plant growth and nitrogen accumulation in C. indica. Therefore, cation supplementation could be an alternative technique to stimulate plant growth and improve nitrate removal in constructed wetlands. Keywords: Constructed wetland, Nitrate removal, Potassium, Tropical wetland plants
摘要：对补充阳离子对美人蕉生长、氮和矿物质积累的影响进行了评估。在Hoagland和Arnon（1950）改良的营养液上生长了类似大小的45天龄植物。添加不同的阳离子以产生6个处理（n=4）：（i）对照（未添加阳离子），（ii）2.5 mM K+，（iii）2.5 mM Ca2+，（iv）75 mM Na+，（v）1.25 mM K++1.25 mM Ca2+和（vi）2.5 mMCa2++75 mM Na+。在温室里进行了为期49天的实验。研究发现，补充K+和K++Ca2+可提高植物生长和总生物量。在接受补充K+的植物中发现最高的SER。相反，在Na+或Na++Ca2+补充的植物中，SERs、叶面积和总生物量降低。在补充K+和K++Ca2+的植物中，NO3-的累积浓度（在整个植物水平上）也最高。补充K+、Ca2+和K++Ca2+的植物的总氮积累高于对照植物。结果表明，补充阳离子特别是K+能促进籼稻植株生长和氮素积累。因此，在人工湿地中，阳离子补充可能是刺激植物生长和提高硝酸盐去除率的一种替代技术。关键词：人工湿地，硝酸盐去除，钾，热带湿地植物
{"title":"Effects of Supplemental Cations on Growth and Nitrogen Accumulation in Canna indica L.","authors":"Manutsawan Manokieng, A. Jampeetong","doi":"10.12982/cmujns.2021.075","DOIUrl":"https://doi.org/10.12982/cmujns.2021.075","url":null,"abstract":"Abstract The effects of supplemental cations on growth, nitrogen, and mineral accumulation were assessed in Canna indica L. Similar sized 45 days-old plants were grown on a nutrient solution modified from Hoagland and Arnon (1950). The different cations were added to generate 6 treatments (n=4): (i) control (no cation added), (ii) 2.5 mM K+, (iii) 2.5 mM Ca2+, (iv) 75 mM Na+, (v) 1.25 mM K+ + 1.25 mM Ca2+ and (vi) 2.5 mM Ca2+ + 75 mM Na+, respectively. An experiment was carried out in the greenhouse for 49 days. The study found that supplemental K+ and K++ Ca2+ increased plant growth and total biomass. The highest SER was found in plants receiving supplemental K+. In contrast, SERs, leaf areas, and total biomass decreased in Na+ or Na++Ca2+ supplemented plants. The accumulated NO3- concentration (at the whole plant level) was also highest in the plants with supplemental K+ and K++Ca2+. The total nitrogen accumulation was higher in the K+, Ca2+, and K++Ca2+ supplemented plants than in the control plants. The results suggest that supplemental cations particularly K+ can enhance plant growth and nitrogen accumulation in C. indica. Therefore, cation supplementation could be an alternative technique to stimulate plant growth and improve nitrate removal in constructed wetlands. Keywords: Constructed wetland, Nitrate removal, Potassium, Tropical wetland plants","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42939999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.12982/cmujns.2021.086
Tanapong Suriyakaew, A. Jampeetong
Abstract In constructed wetlands (CWs), plants are usually affected by low O2 levels. Under such conditions, most soluble iron is reduced to ferrous (Fe2+) which is highly soluble, and toxic to plants as well. As a consequence of excessive ferrous iron with low O2 supply, plant growth is reduced, leading to declining nutrient removal efficiency. This study was conducted to determine the effects of different dissolved oxygen levels (normoxia and hypoxia) with Fe supplied on growth, morphology, and root anatomy of two wetland plants (Canna indica and Heliconia psittacorum). The plants were grown on a nutrient solution modified from Smart and Barko (1985) under normoxic and hypoxic conditions. All plants were grown in greenhouse conditions for 42 days. Plant growth rates and biomass accumulation were drastically reduced under hypoxia while leaf number was not affected. Under hypoxia, root diameter and root porosity also increased in C. indica, whereas H. psittacorum had greater aerenchyma formation. Moreover, C. indica showed adaptive traits to cope with hypoxia and Fe stress by increasing radial oxygen loss (ROL), releasing O2 to the rhizosphere to resist toxic effects of ferrous iron under hypoxia. In contrast, H. psittacorum had no ROL under hypoxia. Moreover, the plants showed leaf chlorosis, leaf roll, and root rotting. Hence, it is suggested that C. indica could have better performance than H. psittacorum to treat wastewater in CWs as this species can adapt to hypoxic conditions and releases O2 into rhizosphere which improves dissolved oxygen (DO) in the wastewater. Keywords: Aerenchyma, Dissolved oxygen, Iron, Root porosity, Wetland emergent plant
{"title":"Effects of Dissolved O2 and Fe Availability on Growth, Morphology, Aerenchyma Formation and Radial Oxygen Loss of Canna indica L. and Heliconia psittacorum L.f.","authors":"Tanapong Suriyakaew, A. Jampeetong","doi":"10.12982/cmujns.2021.086","DOIUrl":"https://doi.org/10.12982/cmujns.2021.086","url":null,"abstract":"Abstract In constructed wetlands (CWs), plants are usually affected by low O2 levels. Under such conditions, most soluble iron is reduced to ferrous (Fe2+) which is highly soluble, and toxic to plants as well. As a consequence of excessive ferrous iron with low O2 supply, plant growth is reduced, leading to declining nutrient removal efficiency. This study was conducted to determine the effects of different dissolved oxygen levels (normoxia and hypoxia) with Fe supplied on growth, morphology, and root anatomy of two wetland plants (Canna indica and Heliconia psittacorum). The plants were grown on a nutrient solution modified from Smart and Barko (1985) under normoxic and hypoxic conditions. All plants were grown in greenhouse conditions for 42 days. Plant growth rates and biomass accumulation were drastically reduced under hypoxia while leaf number was not affected. Under hypoxia, root diameter and root porosity also increased in C. indica, whereas H. psittacorum had greater aerenchyma formation. Moreover, C. indica showed adaptive traits to cope with hypoxia and Fe stress by increasing radial oxygen loss (ROL), releasing O2 to the rhizosphere to resist toxic effects of ferrous iron under hypoxia. In contrast, H. psittacorum had no ROL under hypoxia. Moreover, the plants showed leaf chlorosis, leaf roll, and root rotting. Hence, it is suggested that C. indica could have better performance than H. psittacorum to treat wastewater in CWs as this species can adapt to hypoxic conditions and releases O2 into rhizosphere which improves dissolved oxygen (DO) in the wastewater. Keywords: Aerenchyma, Dissolved oxygen, Iron, Root porosity, Wetland emergent plant","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42683893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.12982/cmujns.2021.091
Orapin Insuan, Benchaluk Thongchuai, R. Chaiwongsa, Supaporn Khamchun, W. Insuan
Abstract Eucalyptus essential oils are used as traditional medicines in many countries. The objective of this study was to evaluate the antioxidant and anti-inflammatory activities of leaf essential oils extracted from three different Eucalyptus species on HepG2 and RAW264.7 cells. Essential oils were distilled from fresh leaf samples, and the chemical constituents were analyzed using gas chromatography–mass spectrometry. The antioxidant activities of essential oils were determined using ABTS and hydroxyl radical scavenging assays, and hydrogen peroxide (H2O2)-induced oxidative stress in HepG2 cells. Additionally, lipopolysaccharide (LPS)-activated RAW264.7 macrophages were used to evaluate the anti-inflammatory properties. The results revealed that Eucalyptus citriodora and Eucalyptus urophylla leaf essential oils had a high content of oxygenated monoterpenes, whereas Eucalyptus deglupta contained a high amount of monoterpene hydrocarbons. Essential oils extracted from the three Eucalyptus species showed antioxidant and anti-inflammatory activities. E. citriodora and E. urophylla leaf essential oils had strong antioxidant activity against H2O2-induced oxidative stress in human HepG2 cells. Additionally, E. citriodora leaf essential oil, which contains a high amount of citronellal, exhibited the most potent anti-inflammatory activity in LPS-activated RAW264.7 macrophages. The antioxidant and anti-inflammatory effects of essential oils depended on their chemical composition. A principal component analysis explained 100% of the variance was performed to construct three groups based on the chemical components and antioxidant and anti-inflammatory activities. This study suggests that E. citriodora leaf essential oil, which represents a good source of oxygenated monoterpenes, could be considered a potential phytochemical agent for the prevention of oxidative stress and inflammation. Keywords: Antioxidant, Anti-inflammatory effect, Eucalyptus essential oils, Gas chromatography–mass spectrometry, Principal component analysis
{"title":"Antioxidant and anti-inflammatory properties of essential oils from three Eucalyptus species","authors":"Orapin Insuan, Benchaluk Thongchuai, R. Chaiwongsa, Supaporn Khamchun, W. Insuan","doi":"10.12982/cmujns.2021.091","DOIUrl":"https://doi.org/10.12982/cmujns.2021.091","url":null,"abstract":"Abstract Eucalyptus essential oils are used as traditional medicines in many countries. The objective of this study was to evaluate the antioxidant and anti-inflammatory activities of leaf essential oils extracted from three different Eucalyptus species on HepG2 and RAW264.7 cells. Essential oils were distilled from fresh leaf samples, and the chemical constituents were analyzed using gas chromatography–mass spectrometry. The antioxidant activities of essential oils were determined using ABTS and hydroxyl radical scavenging assays, and hydrogen peroxide (H2O2)-induced oxidative stress in HepG2 cells. Additionally, lipopolysaccharide (LPS)-activated RAW264.7 macrophages were used to evaluate the anti-inflammatory properties. The results revealed that Eucalyptus citriodora and Eucalyptus urophylla leaf essential oils had a high content of oxygenated monoterpenes, whereas Eucalyptus deglupta contained a high amount of monoterpene hydrocarbons. Essential oils extracted from the three Eucalyptus species showed antioxidant and anti-inflammatory activities. E. citriodora and E. urophylla leaf essential oils had strong antioxidant activity against H2O2-induced oxidative stress in human HepG2 cells. Additionally, E. citriodora leaf essential oil, which contains a high amount of citronellal, exhibited the most potent anti-inflammatory activity in LPS-activated RAW264.7 macrophages. The antioxidant and anti-inflammatory effects of essential oils depended on their chemical composition. A principal component analysis explained 100% of the variance was performed to construct three groups based on the chemical components and antioxidant and anti-inflammatory activities. This study suggests that E. citriodora leaf essential oil, which represents a good source of oxygenated monoterpenes, could be considered a potential phytochemical agent for the prevention of oxidative stress and inflammation. Keywords: Antioxidant, Anti-inflammatory effect, Eucalyptus essential oils, Gas chromatography–mass spectrometry, Principal component analysis","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44334023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.12982/cmujns.2021.079
L. Azis, S. Pinkaew, S. Wichienchot
Abstract The optimal vitamin A (VA) status of lactating women is important for mothers and their breastfed infants, especially in protecting against infectious diseases. Vitamin A fortified rice is one of the food-base intervention strategy which has the potential to improve VA status. Vitamin A and gut microbiota are interrelated in their effect on human health and immunity however no specific relationship has been proved in these groups of population. This study aimed to determine the effect of VA fortified rice on the gut microbiota changes of lactating woman-exclusively breastfed infant pairs. A double-blind, randomized controlled trial (RCT) of VA fortified rice was conducted in 70 lactating women-infants pairs for 14 weeks. Gut microbiota was measured using the fluorescent in situ hybridization (FISH) and next generation sequencing (NGS) technique. Based on the FISH technique, the numbers of Clostridium spp. /Enterobacter spp. were significantly lower (P < 0.05) in mothers fed VA-fortified rice at the end of the study. In contrast, the abundance of Bifidobacterium spp. and Lactobacillus spp. of infants whose mothers fed with VA-fortified rice was significantly higher (P < 0.05) than the control group. The NGS technique confirmed that results with the increasing of Lactobacillus, B. longum and B. Choerinum in the infant of intervention group. In conclusion, VA-fortified rice was efficacious in decreasing Clostridium spp. /Enterobacter spp. in lactating women and raising the number of Bifidobacterium spp. and Lactobacillus spp. in their breastfed infants. Keywords: Breastfeeding, Gut microbiota, Lactating woman-infant pairs, Randomized controlled trial, Vitamin A
{"title":"Effect of Vitamin A-Fortified Rice on the Gut Microbiota of Thai Lactating Women and Their Exclusively Breastfed Infants","authors":"L. Azis, S. Pinkaew, S. Wichienchot","doi":"10.12982/cmujns.2021.079","DOIUrl":"https://doi.org/10.12982/cmujns.2021.079","url":null,"abstract":"Abstract The optimal vitamin A (VA) status of lactating women is important for mothers and their breastfed infants, especially in protecting against infectious diseases. Vitamin A fortified rice is one of the food-base intervention strategy which has the potential to improve VA status. Vitamin A and gut microbiota are interrelated in their effect on human health and immunity however no specific relationship has been proved in these groups of population. This study aimed to determine the effect of VA fortified rice on the gut microbiota changes of lactating woman-exclusively breastfed infant pairs. A double-blind, randomized controlled trial (RCT) of VA fortified rice was conducted in 70 lactating women-infants pairs for 14 weeks. Gut microbiota was measured using the fluorescent in situ hybridization (FISH) and next generation sequencing (NGS) technique. Based on the FISH technique, the numbers of Clostridium spp. /Enterobacter spp. were significantly lower (P < 0.05) in mothers fed VA-fortified rice at the end of the study. In contrast, the abundance of Bifidobacterium spp. and Lactobacillus spp. of infants whose mothers fed with VA-fortified rice was significantly higher (P < 0.05) than the control group. The NGS technique confirmed that results with the increasing of Lactobacillus, B. longum and B. Choerinum in the infant of intervention group. In conclusion, VA-fortified rice was efficacious in decreasing Clostridium spp. /Enterobacter spp. in lactating women and raising the number of Bifidobacterium spp. and Lactobacillus spp. in their breastfed infants. Keywords: Breastfeeding, Gut microbiota, Lactating woman-infant pairs, Randomized controlled trial, Vitamin A","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45933725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.12982/cmujns.2021.087
M. W. Saeed, S. Gillani, Rana Kamran Mahmood, Muhammad Usman
Abstract To assess the efficacy of garlic in comparison with pitavastatin in reducing hypercholesteremia. Databases that include PubMed/Medline, Cochrane, TRIP and SCOPUS were searched. All randomised controlled trials selected for the study. Studies comparing garlic with placebo and pitavastatin with either placebo or other drugs were selected to treat hypercholesteremia. Statistical analysis done using a random-effect model, and results expressed in Mean difference and variance for continuous data with 95% confidence intervals (CI). Twelve studies analysed the efficacy of garlic compared to pitavastatin. Total cholesterol, low-density lipoprotein, high-density lipoprotein, and triglycerides assessed for garlic and pitavastatin's comparative efficacy. Mean difference noticed in total cholesterol in garlic arm was -13.604 with 95% CI = -22.246, -4.962 while in pitavastatin group was -28.205 (95% CI = -29.016,-27.394). The LDL-c reduction not recorded in the garlic arm where the mean increase was recorded of 0.309 (95% CI = -12.502, 13.120) while in the pitavastatin group was -35.538 (95% CI = -39.992, -31.084). Similarly, the increase in HDL was more in the pitavastatin group 5.308 (95% CI = 0.906, 9.710 compared to garlic (2.754 with 95% CI = 1.069, 4.440). Triglycerides level reduction was also low in whereas pitavastatin was -24.210 with 95% CI = -29.249, -19.171.The analysis showed that garlic has a significant effect on lipid profile. The comparison profile of garlic with pitavastatin showed clinical application in the treatment of mild-moderate hypercholesteremia—further studies required the investigator to use the combination therapy in controlled trials. Keywords: Allicin, Allium sativum, Dyslipidemia, Garlic, Hypercholesteremia, Hyperlipidemia Pitavastatin
目的:评价大蒜与匹伐他汀降低高胆固醇血症的疗效。检索了PubMed/Medline、Cochrane、TRIP和SCOPUS等数据库。本研究选择了所有随机对照试验。比较大蒜与安慰剂和匹伐他汀与安慰剂或其他药物治疗高胆固醇血症的研究被选中。采用随机效应模型进行统计分析，连续数据以95%置信区间(CI)的Mean difference and variance表示结果。12项研究分析了大蒜与匹伐他汀的疗效。总胆固醇、低密度脂蛋白、高密度脂蛋白和甘油三酯对大蒜和匹伐他汀比较疗效的评估。大蒜组总胆固醇平均差异为-13.604 (95% CI = -22.246， -4.962)，匹伐他汀组总胆固醇平均差异为-28.205 (95% CI = -29.016，-27.394)。大蒜组的LDL-c降低没有记录，其平均升高记录为0.309 (95% CI = -12.502, 13.120)，而匹伐他汀组的LDL-c降低记录为-35.538 (95% CI = -39.992， -31.084)。同样，匹伐他汀组中HDL的增加比大蒜组(2.754,95% CI = 1.069, 4.440)要多(95% CI = 0.906, 9.710)。甘油三酯水平的降低也很低，而匹伐他汀为-24.210,95% CI = -29.249， -19.171。分析表明，大蒜对血脂有显著影响。大蒜与匹伐他汀的对比显示其在治疗轻中度高胆固醇症中的临床应用——进一步的研究要求研究者在对照试验中使用联合治疗。关键词:大蒜素，大蒜，血脂异常，大蒜，高胆固醇血症，匹伐他汀高脂血症
{"title":"Assessment of the Antihyperlipidemic Effect of Garlic Vs Pitavastatin in Patients with Moderate Hyperlipidemia: A Metanalysis of Randomized Controlled Trials","authors":"M. W. Saeed, S. Gillani, Rana Kamran Mahmood, Muhammad Usman","doi":"10.12982/cmujns.2021.087","DOIUrl":"https://doi.org/10.12982/cmujns.2021.087","url":null,"abstract":"Abstract To assess the efficacy of garlic in comparison with pitavastatin in reducing hypercholesteremia. Databases that include PubMed/Medline, Cochrane, TRIP and SCOPUS were searched. All randomised controlled trials selected for the study. Studies comparing garlic with placebo and pitavastatin with either placebo or other drugs were selected to treat hypercholesteremia. Statistical analysis done using a random-effect model, and results expressed in Mean difference and variance for continuous data with 95% confidence intervals (CI). Twelve studies analysed the efficacy of garlic compared to pitavastatin. Total cholesterol, low-density lipoprotein, high-density lipoprotein, and triglycerides assessed for garlic and pitavastatin's comparative efficacy. Mean difference noticed in total cholesterol in garlic arm was -13.604 with 95% CI = -22.246, -4.962 while in pitavastatin group was -28.205 (95% CI = -29.016,-27.394). The LDL-c reduction not recorded in the garlic arm where the mean increase was recorded of 0.309 (95% CI = -12.502, 13.120) while in the pitavastatin group was -35.538 (95% CI = -39.992, -31.084). Similarly, the increase in HDL was more in the pitavastatin group 5.308 (95% CI = 0.906, 9.710 compared to garlic (2.754 with 95% CI = 1.069, 4.440). Triglycerides level reduction was also low in whereas pitavastatin was -24.210 with 95% CI = -29.249, -19.171.The analysis showed that garlic has a significant effect on lipid profile. The comparison profile of garlic with pitavastatin showed clinical application in the treatment of mild-moderate hypercholesteremia—further studies required the investigator to use the combination therapy in controlled trials. Keywords: Allicin, Allium sativum, Dyslipidemia, Garlic, Hypercholesteremia, Hyperlipidemia Pitavastatin","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44423676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.12982/cmujns.2021.076
R. Kammoolkon, Nutta Taneepanichskul, S. Taneepanichskul
Abstract Informal-sector weavers using indigo-dyed cotton are occupationally exposed to respirable dust which may contain contaminants from chemicals used for pH adjustment in the natural indigo fermentation process. The major health problems associated with respirable dust induce pulmonary function impairment and respiratory disease. However, there have been few studies into the respiratory problems of informal-sector weavers in Thailand. This study investigated the link between occupational respirable dust exposure and pulmonary function among weavers using indigo-dyed cotton in Thailand. A cross-sectional study was conducted of 147 weavers located in Sakon Nakhon province. Respiratory dust (RD) samples were collected from the immediate breathing zone of all weavers. Lung function was measured using a portable spirometer operated by a trained physician and the spirometric results were examined by a pulmonologist before reporting. Most participants were female (98.6%) and the median reported interquartile age (IQR) was 58 (50–62.75) years. Average predicted values for FVC, FEV1 and FEV1/FVC were 92.9% (±20.7), 96.7% (±17.9) and 88.3% (±10.8). Of the respiratory function patterns of the 147 weavers, 20 (13.6%) were restrictive, 11 (7.5%) were obstructive and 1 (0.7%) was combined. The average RD concentration (mean ± SD) was 47.9 ± 28.3 μg/m3. Multivariate linear regression models showed an increase of 1 μg/m3 in RD exposure was associated with a 0.179% lower level of FEV1 (95% confidence interval (CI) -0.278 to -0.080) and a 0.068 % lower level of FEV1/FVC (95% CI -0.128 to -0.008). Our findings suggested that exposure to respirable dust might impair pulmonary function in informal-sector cotton weavers. Keywords: Indigo-dyed Cotton, Informal-sector Weavers, Occupational Exposure, Pulmonary function, Respirable Dust
{"title":"Relationship of Respirable Dust Exposure to Pulmonary Function among Informal-sector Weavers using Indigo-dyed Cotton","authors":"R. Kammoolkon, Nutta Taneepanichskul, S. Taneepanichskul","doi":"10.12982/cmujns.2021.076","DOIUrl":"https://doi.org/10.12982/cmujns.2021.076","url":null,"abstract":"Abstract Informal-sector weavers using indigo-dyed cotton are occupationally exposed to respirable dust which may contain contaminants from chemicals used for pH adjustment in the natural indigo fermentation process. The major health problems associated with respirable dust induce pulmonary function impairment and respiratory disease. However, there have been few studies into the respiratory problems of informal-sector weavers in Thailand. This study investigated the link between occupational respirable dust exposure and pulmonary function among weavers using indigo-dyed cotton in Thailand. A cross-sectional study was conducted of 147 weavers located in Sakon Nakhon province. Respiratory dust (RD) samples were collected from the immediate breathing zone of all weavers. Lung function was measured using a portable spirometer operated by a trained physician and the spirometric results were examined by a pulmonologist before reporting. Most participants were female (98.6%) and the median reported interquartile age (IQR) was 58 (50–62.75) years. Average predicted values for FVC, FEV1 and FEV1/FVC were 92.9% (±20.7), 96.7% (±17.9) and 88.3% (±10.8). Of the respiratory function patterns of the 147 weavers, 20 (13.6%) were restrictive, 11 (7.5%) were obstructive and 1 (0.7%) was combined. The average RD concentration (mean ± SD) was 47.9 ± 28.3 μg/m3. Multivariate linear regression models showed an increase of 1 μg/m3 in RD exposure was associated with a 0.179% lower level of FEV1 (95% confidence interval (CI) -0.278 to -0.080) and a 0.068 % lower level of FEV1/FVC (95% CI -0.128 to -0.008). Our findings suggested that exposure to respirable dust might impair pulmonary function in informal-sector cotton weavers. Keywords: Indigo-dyed Cotton, Informal-sector Weavers, Occupational Exposure, Pulmonary function, Respirable Dust","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49553984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.12982/cmujns.2021.092
Alemu Bekele Eticha
Abstract Despite advancements in HIV/AIDS prevention and treatment, HIV continues to be a global problem. Antiretroviral therapy is a critical treatment that has been used to treat HIV-infected patients since 1996. Even though an increase in the number of patients enrolled in ART, the mortality rate for HIV cases in Ethiopia has never been overcome. Thus, this study aimed to identify influential factors of death of HIV-infected individuals received antiretroviral therapy at the Tepi General Hospital. The secondary data was extracted from each selected patient for whom the ART was initiated from September 2011 to June 2018. Then, Cox regression technique provided the essential determinants of time to death of HIV-infected patients. The findings revealed that 35.14 percent of HIV patients died despite being on ART. The identified causes of death were being over 40 years old, being in clinical stage IV, being uneducated, having a low body weight, and having a low CD4 cell count. Gender, tuberculosis status, and functional status, on the other hand, were not supported as factors. Thus, age over 40 years, being underweight, having a low baseline CD4 cell count, being in an advanced WHO clinical stage, and having a low education level were identified as critical risk factors that exposed to early death even while on ART. As a result, the hospital advised prioritizing patients based on the identified factors. Keywords: AIDS; Analysis; Biological modeling; Biological activities
{"title":"Survival Analysis of Time to Death of HIV-Infected Patients under Antiretroviral Therapy in Tepi General Hospital, South West Ethiopia","authors":"Alemu Bekele Eticha","doi":"10.12982/cmujns.2021.092","DOIUrl":"https://doi.org/10.12982/cmujns.2021.092","url":null,"abstract":"Abstract Despite advancements in HIV/AIDS prevention and treatment, HIV continues to be a global problem. Antiretroviral therapy is a critical treatment that has been used to treat HIV-infected patients since 1996. Even though an increase in the number of patients enrolled in ART, the mortality rate for HIV cases in Ethiopia has never been overcome. Thus, this study aimed to identify influential factors of death of HIV-infected individuals received antiretroviral therapy at the Tepi General Hospital. The secondary data was extracted from each selected patient for whom the ART was initiated from September 2011 to June 2018. Then, Cox regression technique provided the essential determinants of time to death of HIV-infected patients. The findings revealed that 35.14 percent of HIV patients died despite being on ART. The identified causes of death were being over 40 years old, being in clinical stage IV, being uneducated, having a low body weight, and having a low CD4 cell count. Gender, tuberculosis status, and functional status, on the other hand, were not supported as factors. Thus, age over 40 years, being underweight, having a low baseline CD4 cell count, being in an advanced WHO clinical stage, and having a low education level were identified as critical risk factors that exposed to early death even while on ART. As a result, the hospital advised prioritizing patients based on the identified factors. Keywords: AIDS; Analysis; Biological modeling; Biological activities","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47031736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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