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Comparative analysis of four edible mushrooms based on HPLC fingerprint and pattern recognition analysis 基于HPLC指纹图谱和模式识别分析的四种食用菌的比较分析
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-06 DOI: 10.1016/j.jfutfo.2023.05.008
Xiali Si , Xinyu Liu , Shuangyu Shao, Zhiyong Du, Pengfei Tu, Qingying Zhang

A high performance liquid chromatography-ultraviolet (HPLC-UV) fingerprint method for the overall chemical analysis of edible mushrooms was established based on Auricularia heimuer for the first time, and then applied to analyze 60 batches of A. heimuer, Auricularia cornea, Auricularia cornea ʻYu Muerʼ and Tremella fuciformis. A total of 9 characteristic peaks of A. heimuer, 11 characteristic peaks of A. cornea, 6 characteristic peaks of A. cornea ʻYu Muerʼ, and 9 characteristic peaks of T. fuciformis were designated. Then, a combinatory analysis, including similarity evaluation, hierarchical cluster analysis and principal component analysis, revealed the chemical consistency and difference between samples from the same and different species. The HPLC fingerprint method established in this paper could be used to characterize the components of A. heimuer, A. cornea, A. cornea ʻYu Muerʼ, and T. fuciformis and discriminate the 4 edible mushrooms effectively in combination with pattern recognition analysis.

以黑木耳为原料,首次建立了食用菌全化学分析的高效液相色谱-紫外(HPLC-UV)指纹图谱方法,并对60批黑木耳、角膜黑木耳、眼角膜黑木耳和银耳进行了分析。共鉴定出黑木耳9个特征峰、角膜11个特征峰,角膜6个特征峰和岩藻9个特征峰值。然后,通过相似性评价、层次聚类分析和主成分分析等组合分析,揭示了同一物种和不同物种样品之间的化学一致性和差异性。本文建立的高效液相色谱指纹图谱方法可用于黑木耳、角膜蘑菇、角膜蘑菇和岩藻的成分鉴定,并结合模式识别分析对4种食用菌进行有效鉴别。
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引用次数: 0
Establishment of enzyme-linked immunosorbent assay for beef and lamb contents in cooked meat 牛肉和羊肉熟肉中含量的酶联免疫吸附测定方法的建立
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-06 DOI: 10.1016/j.jfutfo.2023.05.010
Yujing Li , Jingjing Liu , Sufang Fan , Zhao Li , Jing Zhang , Erjing Zhang , Ziran Li , Yan Zhang , Chunsheng Li

In this study, an enzyme-linked immunosorbent assay (ELISA) was established to detect beef and lamb components, and its performance was tested. Double-antibody sandwich ELISA was adopted and determined a coating concentration of capture antibody 3G5 of 1:4 000, a working concentration of enzyme-labeled antibody 2E7-horseradish peroxidase (HRP) of 1:1 000, a sample incubation time of 60 min and a detection antibody reaction time of 60 min. The specificity, sensitivity, repeatability and stability of this assay were determined. The limit of detection for beef and lamb skeletal muscle troponin I was 45 mg/kg, the inter-assay and intra-assay recovery rates ranged from 80.4% to 115.7%, the coefficients of variation were below 13.6%, and the cross reaction rates of the tissue components of chicken, duck and fish were below 13.4%. The sandwich ELISA method established in this study is stable and has high accuracy. The test results were consistent with the polymerase chain reaction (PCR) method at 50 and 100 g/kg. Therefore, this ELISA method can be used to quantitatively detect beef and lamb components in meat products.

本研究建立了一种检测牛肉和羊肉成分的酶联免疫吸附试验(ELISA),并对其性能进行了测试。采用双抗体夹心ELISA法测定捕获抗体3G5的包被浓度为1:4000,酶标记抗体2E7辣根过氧化物酶(HRP)的工作浓度为1:1000,样品孵育时间为60min,检测抗体反应时间为60min。牛肉和羊肉骨骼肌肌钙蛋白I的检测限为45mg/kg,批间和批内回收率在80.4%-115.7%之间,变异系数在13.6%以下,鸡、鸭和鱼组织成分的交叉反应率在13.4%以下。在50和100g/kg下,检测结果与聚合酶链式反应(PCR)方法一致。因此,该ELISA方法可用于肉制品中牛肉和羊肉成分的定量检测。
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引用次数: 0
Optical sensor array for the discrimination of liquors 用于鉴别酒的光学传感器阵列
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-06 DOI: 10.1016/j.jfutfo.2023.05.004
Yang Yu , Fangfang Shi , Yi Zhang, Fei Li, Jinsong Han

The discrimination of liquors plays a vital role in our daily life as a large amount of cheap alcoholic beverages and low-quality counterfeits greatly damage the health of consumers. Array-based optical sensors have been developed as a fast and effective tool to discriminate multi-analytes or complex mixtures with similar structures, such as acids, esters, aldehydes and ketones. Over the past decades, optical sensor arrays have demonstrated remarkable capabilities in fast and accurate detection of diverse types, qualities, and authentic liquors. The identification strategy mainly focuses on the specific/non-specific interactions of flavor compounds in liquors with sensor elements. The cross-reactive optical signal readouts from the combination of sensor elements with liquors and multiple machine learning algorithms are essential to ensure the discriminatory capability of sensing system. In this review, we discuss the advantages and recent advances in pattern recognition of liquors in terms of the design of various types of sensing materials and the construction strategies of sensor arrays.

白酒的鉴别在我们的日常生活中起着至关重要的作用,因为大量的廉价酒精饮料和劣质假冒产品极大地损害了消费者的健康。基于阵列的光学传感器已经发展成为一种快速有效的工具,用于区分多种分析物或具有相似结构的复杂混合物,如酸、酯、醛和酮。在过去的几十年里,光学传感器阵列在快速准确地检测不同类型、质量和正宗酒方面表现出了非凡的能力。识别策略主要集中在酒中风味化合物与传感器元件的特异性/非特异性相互作用上。传感器元件与酒相结合的交叉反应光信号读出与多种机器学习算法相结合是保证传感系统判别能力的关键。本文从传感材料的设计和传感器阵列的构建策略等方面综述了白酒模式识别的优点和最新进展。
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引用次数: 0
Simultaneous determination of 6 carbamate pesticides and relevant metabolites in oilseeds and oil with the modified fast QuECHERS method by ultra-performance liquid chromatography-tandem mass spectrometry 改进的快速QuECHERS超高效液相色谱-串联质谱法同时测定油籽和油脂中6种氨基甲酸酯类农药及其代谢物
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-06 DOI: 10.1016/j.jfutfo.2023.05.009
Bangben Yao , Yun Li , Ru Zhang , Li Yao , Jianguo Xu , Chao Yan , Wei Chen

A sensitive and reliable method was developed and validated for the simultaneous determination of 6 carbamate pesticides and relevant metabolites based on the modified fast QuEChERS (quick, easy, cheap, effective, rugged and safe) method combined with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The method was also applied in the analysis of 23 commonly consumed oilseeds and oil samples. The sample was extracted with acetonitrile for three times and then the extracted solution was filtered directly through 250 mg precipitated silica adsorbent (PSA) and 250 mg C18. The mobile phase was water (containing 0.1% formic acid) and acetonitrile. The external standard protocols were adopted to validate the method in terms of recovery, linearity, precision, sensitivity, and relative standard deviation (RSD) under the optimized conditions. The linear calibration curves with different ranges were established with R2 > 0.997 3, limit of detections (LODs) ranged from 0.100 µg/kg to 2.000 µg/kg and limit of quantitations (LOQs) ranged from 0.300 µg/kg to 6.000 µg/kg. The limit of detection meets the detection requirement for the maximum residue limits in practical samples. Meanwhile, the recoveries ranged from 60.2% to 116.6% with associated RSDs ranged from 0.33% to 5.35% in 10 oilseeds matrices and ranged from 61.9% to 116.9% with RSDs ranged from 0.30% to 5.02% in 13 oil matrices. These features do make the UPLC-MS/MS an ideal analytical platform for accurate and high throughput analysis of carbamates residues in the complex matrix.

基于改进的快速QuEChERS(快速、简便、廉价、有效、稳定、安全)方法,结合超高效液相色谱-串联质谱(UPLC-MS/MS),建立并验证了一种同时测定6种氨基甲酸酯类农药及其代谢产物的灵敏可靠方法。该方法还应用于23种常用油籽和油料样品的分析。样品用乙腈提取三次,然后通过250mg沉淀二氧化硅吸附剂(PSA)和250mg C18直接过滤提取的溶液。流动相为水(含0.1%甲酸)和乙腈。在优化的条件下,采用外标方案对该方法的回收率、线性、精密度、灵敏度和相对标准偏差(RSD)进行了验证。建立了具有不同范围的线性校准曲线,其中R2>;0.9973,检测限(LOD)范围为0.100µg/kg至2.000µg/kg,定量限(LOQ)范围为0.300µmg/kg至6.000µg/kg。检测限满足实际样品中最大残留限量的检测要求。同时,在10种油籽基质中,回收率为60.2%至116.6%,相关RSD为0.33%至5.35%,在13种油基质中,RSD为61.9%至116.9%,相关RSD为0.30%至5.02%。这些特性确实使UPLC-MS/MS成为精确和高通量分析复杂基质中氨基甲酸酯残留物的理想分析平台。
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引用次数: 0
Paper-based biosensors based on multiple recognition modes for visual detection of microbially contaminated food 基于多种识别模式的纸质生物传感器对微生物污染食品的视觉检测
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-06 DOI: 10.1016/j.jfutfo.2023.05.007
Jie Li , Keren Chen , Yuan Su , Longjiao Zhu , Hongxing Zhang , Wentao Xu , Xiangyang Li

Microbially contaminated food can cause serious health hazards and economic losses, therefore sensitive, rapid, and highly specific visual detection is called for. Traditional detection of microorganisms is complex and time-consuming, which cannot meet current testing demands. The emergence of paper-based biosensors provided an effective method for efficient and visual detection of microorganisms, due to its high speed, all-in-one device, low cost, and convenience. This review focused on 5 biomarkers, namely nucleic acids, proteins, lipopolysaccharides, metabolites, and the whole microorganism of microorganisms. Besides, the recognition methods were summed up in 5 forms, including immunological recognition, aptamer recognition, nucleic acid amplification-mediated recognition, DNAzyme recognition and clustered regularly interspaced short palindromic repeats mediated recognition. In addition, we summarized the applications of paper-based biosensors in the detection of microorganisms thoroughly. Through the exploration of different biomarkers, identification methods, and applications, we hope to provide a reference for the development of paper-based biosensors and their application in safeguarding the food chain.

受微生物污染的食品会造成严重的健康危害和经济损失,因此需要灵敏、快速、特异性强的视觉检测。传统的微生物检测既复杂又耗时,无法满足当前的检测需求。纸基生物传感器的出现提供了一种高效、直观地检测微生物的有效方法,因为它具有高速、一体式设备、低成本和方便性。综述了5种生物标志物,即核酸、蛋白质、脂多糖、代谢产物和微生物的整个微生物。此外,将识别方法归纳为5种形式,包括免疫识别、适体识别、核酸扩增介导的识别、DNA酶识别和聚类规则间隔短回文重复序列介导的辨识。此外,我们还全面总结了纸基生物传感器在微生物检测中的应用。通过对不同生物标志物、识别方法和应用的探索,我们希望为纸基生物传感器的发展及其在保障食物链中的应用提供参考。
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引用次数: 0
Plant based metabolomics: a future prospective and versatile tool for metabolite databases of Curcuma longa 植物代谢组学:姜黄代谢产物数据库的一种未来前景和多功能工具
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-06 DOI: 10.1016/j.jfutfo.2023.05.003
Shuvendhu Gupta , Amrat Pal Singh , Gurpreet Singh , Xianting Ding , Alok Sharma

Sophisticated analytical instruments are used for quality evaluation and scientific validation to compliance the market demands. Recently, increase in demand of Curcuma longa has led to a high swing in small and medium food industry. The present review is devoted to an in-depth information on metabolite database of C. longa from different analytical techniques such as high-performance thin-layer chromatography (HPTLC), liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry and nuclear magnetic resonance. This work highlights different extraction methods, phytochemistry along with structure activity relationship, nutritional profiling, market viability and pharmacology of C. longa. The bioactives of C. longa depends on various factors such as plants growth, temperature, humidity, soil type, collection, drying and extraction process. These factors can contribute in quality evaluation of the food supplements if need to be included. The introduction of omics approaches can helpful to explore the quality and identification of potential lead of C. longa. The results could play an essential role in the area of food-phyto based profiling. Further, metabolomics techniques may provide an analytical platform by combination of different “omics” data sources for future research to food and herbal based products.

先进的分析仪器用于质量评估和科学验证,以满足市场需求。近年来,姜黄需求的增加导致中小型食品行业出现了高波动。本文从高效薄层色谱(HPTLC)、液相色谱-质谱、气相色谱-色谱和核磁共振等不同分析技术对龙舌兰的代谢产物数据库进行了深入的研究。这项工作重点介绍了龙舌兰的不同提取方法、植物化学以及结构-活性关系、营养概况、市场可行性和药理学。龙舌兰的生物活性取决于植物生长、温度、湿度、土壤类型、采集、干燥和提取过程等因素。若需要将这些因素包括在内,这些因素有助于食品补充剂的质量评估。组学方法的引入有助于探索龙舌兰潜在铅的质量和鉴定。该结果可能在基于食物植物的分析领域发挥重要作用。此外,代谢组学技术可以通过组合不同的“组学”数据源,为未来对食品和草药产品的研究提供一个分析平台。
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引用次数: 1
Inorganic virus-like nanoparticles for biomedical applications: a minireview 生物医学应用的无机类病毒纳米颗粒:综述
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-06 DOI: 10.1016/j.jfutfo.2023.05.006
Liying Zhao , Jiahong Zhou , Dawei Deng

Nature has the ingenious capability to design spiky topological features at the macro-and nanoscales, which exhibits fascinating interface adhesive properties by means of multivalent interactions. Following a biomimetic approach, such as nanoscale virus particles are highly infectious toward host cells, a range of organic and inorganic spiky particles (virus-like nanostructures) have been precisely engineered for diverse biomedical applications. Generally, organic virus-like particles (VLPs) derived from viral capsids (often termed as virosomes) have been extensively studied and reviewed, but concomitant concerns regarding immunogenicity and risks of mutagenesis limit clinical potential of organic VLPs. In contrast, inorganic VLPs (viral-mimicking topography) possess fascinating physicochemical characteristics, such as excellent electrical, optical, magnetic, mechanical and catalytic properties, which make them particularly suitable for biomedical applications. Alternatively, there is no comprehensive review related to inorganic VLPs engineered with non-viral shell for biomedical applications. Hence, in this review, we present a brief overview on inorganic VLPs, followed by summarizing the construction and properties of virus-like nanostructures, as well as the mechanisms of nano-bio interface interactions initiated by spiky topography. Furthermore, we focus on the recent advances of VLPs for biomedical applications (including biosensing, antibacterial therapy and cancer treatment). Finally, the future outlook and emerging challenges will be presented. This review aims to provide future scope of the rational design of inorganic non-viral vectors, especially with respect to gene-based therapy platforms.

大自然有能力在宏观和纳米尺度上设计尖锐的拓扑特征,通过多价相互作用展现出迷人的界面粘合特性。根据仿生方法,例如纳米级病毒颗粒对宿主细胞具有高度传染性,一系列有机和无机尖峰颗粒(病毒样纳米结构)已被精确设计用于各种生物医学应用。通常,来源于病毒衣壳(通常称为病毒体)的有机病毒样颗粒(VLP)已被广泛研究和综述,但随之而来的免疫原性和诱变风险限制了有机VLP的临床潜力。相比之下,无机VLP(病毒模拟形貌)具有迷人的物理化学特性,如优异的电学、光学、磁学、机械和催化性能,这使它们特别适合生物医学应用。或者,还没有关于用非病毒外壳设计的用于生物医学应用的无机VLP的全面综述。因此,在这篇综述中,我们简要概述了无机VLP,然后总结了病毒样纳米结构的构建和性质,以及由尖峰形貌引发的纳米-生物界面相互作用的机制。此外,我们还重点介绍了VLP在生物医学应用(包括生物传感、抗菌治疗和癌症治疗)方面的最新进展。最后,将介绍未来前景和新出现的挑战。这篇综述旨在为无机非病毒载体的合理设计提供未来的范围,特别是在基于基因的治疗平台方面。
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引用次数: 0
Biogenic amines detection in meat and meat products: the mechanisms, applications, and future trends 肉和肉制品中的生物胺检测:机制、应用和未来趋势
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-06 DOI: 10.1016/j.jfutfo.2023.05.002
Yanan Liu , Yongqiang He , Huan Li , Donghui Jia , Linglin Fu , Jian Chen , Dequan Zhang , Yanbo Wang

Biogenic amine is one of the main categories of hazardous compounds in meat and meat products, making its detection methods vital for the assurance of edible safety. In this sense, many biogenic amine detection techniques such as chromatographic, electrophoretic, and electrochemical methods have been developed, which play an irreplaceable role in ensuring the safety of meat and meat products. Due to the increasing demand for fast and on-site detection techniques, visual detection methods have been gradually developed compared with non-visual methods such as chromatography and electrophoresis. Herein, we comprehensively review the mechanism and the latest progress of these biogenic amine detection methods; besides, we put forward the prospects for the future development of biogenic amine detection techniques, with a view to providing support for the establishment of more accurate and efficient detection, prevention and control strategies of biogenic amines.

生物胺是肉和肉制品中的主要危险化合物之一,其检测方法对保证食用安全至关重要。从这个意义上说,已经开发了许多生物胺检测技术,如色谱、电泳和电化学方法,这些技术在确保肉和肉制品的安全方面发挥着不可替代的作用。由于对快速和现场检测技术的需求不断增加,与色谱和电泳等非视觉方法相比,视觉检测方法逐渐发展起来。在此,我们全面综述了这些生物胺检测方法的机理和最新进展;此外,我们对生物胺检测技术的未来发展进行了展望,以期为建立更准确、高效的生物胺检测、预防和控制策略提供支持。
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引用次数: 4
Recent advances in magnetic molecularly imprinted polymers and their application in the food safety analysis 磁性分子印迹聚合物研究进展及其在食品安全分析中的应用
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-06 DOI: 10.1016/j.jfutfo.2023.05.001
Beibei Jia , Feng Feng , Xiujuan Wang , Yaxuan Song , Feng Zhang

Food safety is a worldwide concern and is directly related to human health. Therefore, convenient, effective, and economical methods and technologies for food safety analysis have been developed continuously. Magnetic molecularly imprinted polymers (MMIPs) have gained extensive attention in recent years, as they have high selectivity, high adsorption capacity, and are easy to isolate from food samples. Recently, advanced strategies for the synthesis of MMIPs have been proposed to solve problems of template leakage and non-specific adsorption, and to increase the biocompatibility, adsorption rate, as well as adsorption capacity of the imprinted materials. In this review, we focus on new attempts at modification of magnetic core and MMIPs’ surfaces, and the selection of template, functional monomer, cross-linker as well as porogen. Studies are summarized that used advanced MMIPs for the recognition and adsorption of pesticide residues, veterinary drug residues, mycotoxins, contaminants, and adulterations in foodstuffs over the last 5 years. Finally, some still existing challenges and future prospects to further promote MMIPs properties are also discussed.

食品安全是全世界关注的问题,直接关系到人类健康。因此,方便、有效、经济的食品安全分析方法和技术不断发展。近年来,磁性分子印迹聚合物(MMIP)因其具有高选择性、高吸附能力以及易于从食品样品中分离而受到广泛关注。最近,人们提出了合成MMIP的先进策略,以解决模板泄漏和非特异性吸附的问题,并提高印迹材料的生物相容性、吸附速率和吸附能力。在这篇综述中,我们重点介绍了磁芯和MMIP表面修饰的新尝试,以及模板、功能单体、交联剂和致孔剂的选择。总结了过去5年来使用先进MMIP识别和吸附食品中的农药残留、兽药残留、真菌毒素、污染物和掺假的研究。最后,还讨论了进一步提高MMIP性能的一些仍然存在的挑战和未来前景。
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引用次数: 0
A metabolomics approach for monitoring thermal processing temperature of bovine milk using ultra-performance liquid chromatography tandem Q-Exactive mass spectrometry and multivariate data analysis 利用超高效液相色谱串联Q-Exactive质谱和多变量数据分析的代谢组学方法监测牛乳的热加工温度
Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-06 DOI: 10.1016/j.jfutfo.2023.05.005
Yun Xie , Xuemei Nie , Chang Wang , Xiuli Xu , Feng Zhang

Due to the lack of effective assessment method, overheated milk commodities are often marketed as pasteurized milk on the market, which was sold in high price by fraud. Thus, this article aims to establish an approach based on metabolomics to monitor thermal processing temperature of bovine milk. Metabolomics data of bovine milk samples heated at temperatures ranging from 60 °C to 150 °C were achieved by ultra-performance liquid chromatography-high-resolution mass spectrometry (UPLC-HRMS) platform, followed by multivariate data analysis. A regular variation pattern of chemical composition as temperature rises was pictured, furthermore, Nε-carboxymethyl lysine (CML), Nε-carboxyethyl lysine (CEL), pentosidine, pyrraline and lysinoalanine (LAL) were identified as 5 of the most contributed compounds to discriminate pasteurized and ultra-high-temperature (UHT) milk. By the comprehensive study on their content changes, we concluded that the optimal temperature range was 90−100 °C for the generation of CML and CEL in this experiment, moreover, 110−120 °C for LAL, 80−100 °C for pentosidine and 130−140 °C for pyrraline. Finally, a predicted rule to discriminate pasteurized and UHT milk was preliminarily established based on the ratios of CML/CEL, CEL/pentosidine and CML/pentosidine, which could be applied in food labelling authentication of commercial bovine milk after further validation.

由于缺乏有效的评估方法,过热牛奶商品在市场上经常被当作巴氏杀菌乳销售,并通过欺诈手段高价出售。因此,本文旨在建立一种基于代谢组学的牛奶热加工温度监测方法。通过超高效液相色谱-高分辨率质谱(UPLC-HRMS)平台,然后进行多变量数据分析,获得了在60°C至150°C温度范围内加热的牛乳样品的代谢组学数据。化学成分随温度升高呈规律变化,Nε-羧甲基赖氨酸(CML)、Nε-羧基乙基赖氨酸。通过对其含量变化的综合研究,我们得出本实验产生CML和CEL的最佳温度范围为90−100°C,此外,LAL为110−120°C,戊糖苷为80−100°C.吡拉林为130−140°C。最后,根据CML/CEL、CEL/戊糖苷和CML/戊糖苷的比值,初步建立了区分巴氏灭菌乳和超高温灭菌乳的预测规则,经进一步验证,可用于商品牛乳的食品标签认证。
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引用次数: 1
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Journal of Future Foods
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