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Nano-enabled stress-smart agriculture: Can nanotechnology deliver drought and salinity-smart crops? 纳米压力智能农业:纳米技术能提供干旱和盐碱智能作物吗?
Pub Date : 2023-07-18 DOI: 10.1002/sae2.12061
Ali Raza, Sidra Charagh, Hajar Salehi, Saghir Abbas, Faisal Saeed, Gérrard E. J. Poinern, Kadambot H. M. Siddique, Rajeev K. Varshney

Salinity and drought stress substantially decrease crop yield and superiority, directly threatening the food supply needed to meet the rising food needs of the growing total population. Nanotechnology is a step towards improving agricultural output and stress tolerance by improving the efficacy of inputs in agriculture via targeted delivery, controlled release, and enhanced solubility and adhesion while also reducing significant damage. The direct application of nanoparticles (NPs)/nanomaterials can boost the performance and effectiveness of physio-biochemical and molecular mechanisms in plants under stress conditions, leading to advanced stress tolerance. Therefore, we presented the effects and plant responses to stress conditions, and also explored the potential of nanomaterials for improving agricultural systems, and discussed the advantages of applying NPs at various developmental stages to alleviate the negative effects of salinity and drought stress. Moreover, we feature the recent innovations in state-of-the-art nanobiotechnology, specifically NP-mediated genome editing via CRISPR/Cas system, to develop stress-smart crops. However, further investigations are needed to unravel the role of nanobiotechnology in addressing climate change challenges in modern agricultural systems. We propose that combining nanobiotechnology, genome editing and speed breeding techniques could enable the designing of climate-smart cultivars (particularly bred or genetically modified plant varieties) to meet the food security needs of the rising world population.

盐碱和干旱胁迫大大降低了作物产量和优势,直接威胁到满足不断增长的总人口日益增长的粮食需求所需的粮食供应。纳米技术是朝着提高农业产量和耐压力性迈出的一步,它通过靶向递送、控制释放、增强溶解性和粘附性来提高农业投入的效力,同时减少重大损害。纳米颗粒/纳米材料的直接应用可以提高植物在胁迫条件下的物理、生物化学和分子机制的性能和有效性,从而提高植物的抗逆性。因此,我们介绍了植物对胁迫条件的影响和反应,还探索了纳米材料在改善农业系统方面的潜力,并讨论了在不同发育阶段应用纳米材料以减轻盐度和干旱胁迫的负面影响的优势。此外,我们还介绍了最先进的纳米生物技术的最新创新,特别是通过CRISPR/Cas系统进行NP介导的基因组编辑,以开发胁迫智能作物。然而,还需要进一步的研究来揭示纳米生物技术在应对现代农业系统中气候变化挑战方面的作用。我们建议,将纳米生物技术、基因组编辑和快速育种技术相结合,可以设计气候智能型品种(特别是育种或转基因植物品种),以满足不断增长的世界人口的粮食安全需求。
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引用次数: 11
Impact of multiple soil microbial inoculants on biomass and biomass allocation of the legume crop field pea (Fabaceae: Pisum sativum L.) 多种土壤微生物接种剂对豆科豌豆生物量和生物量分配的影响
Pub Date : 2023-07-12 DOI: 10.1002/sae2.12060
Ben Glogoza, Laura Aldrich-Wolfe, Jarrad R. Prasifka, Deirdre A. Prischmann-Voldseth

Introduction

Food production is a global challenge and consequently there is considerable interest in manipulating the rhizobiome using microbial inoculants (MI) to support sustainable agriculture. The effectiveness of MI needs to be evaluated under diverse environmental conditions, especially for nitrogen-fixing legume crops, for which interactions between their associated bacteria and other soil biota may be particularly important.

Materials and Methods

We investigated how three commercially available types of plant growth-promoting MI, alone and in combination (B5: five species of Bacillus bacteria, GP: four species of Trichoderma fungi, N2: Paenibacillus polymyxa bacteria) impacted field pea (Fabales: Fabaceae, Pisum sativum L.) in the greenhouse and a 2-year field experiment.

Results

In the greenhouse, we found that effects of MI on plant performance varied, with positive effects of MI only apparent when plants were grown in the winter and likely under greater stress, because they lacked nodules. Plants grown in the summer had nodules and 2-week-old MI plants had less root biomass and total plant weight than noninoculated controls, but the weight of 4-week-old MI plants was similar to or greater than that of the controls. In the field, the root-to-shoot biomass ratio was highest in noninoculated controls and positive effects of N2 on shoots and B5 on shoots and pod densities did not translate into differences in pod weight or total plant weight. In most cases, plants inoculated with all three inoculants performed similar to those receiving a single inoculant, whereas root colonization by arbuscular mycorrhizal fungi was higher for B5 plants than plants in the other treatments.

Conclusions

Overall, B5 was the inoculant most often associated with increased plant biomass. This research underscores the need to consider microbial and environmental context when evaluating MI.

引言粮食生产是一个全球性的挑战,因此人们对使用微生物接种剂(MI)来控制根生物群落以支持可持续农业非常感兴趣。MI的有效性需要在不同的环境条件下进行评估,特别是对于固氮豆类作物,其相关细菌和其他土壤生物群之间的相互作用可能特别重要。材料和方法我们研究了三种市售的促进植物生长的MI,单独和组合(B5:五种芽孢杆菌,GP:四种木霉,N2:多粘菌Paenibacillus)在温室中影响豌豆(Fabales:Fabaceae,Pisum sativum L.)和2年的田间试验。结果在温室中,我们发现MI对植物性能的影响各不相同,只有当植物在冬季生长时,MI的积极影响才明显,并且可能在更大的压力下生长,因为它们缺乏根瘤。夏季生长的植物有根瘤,2周龄的MI植物的根生物量和植物总重量比未接种的对照组少,但4周龄的MI-植物的重量与对照组相似或更大。在田间,未接种对照的根与地上部生物量比最高,N2对地上部和B5对地上部的积极影响以及荚密度没有转化为荚重或植株总重量的差异。在大多数情况下,用所有三种接种物接种的植物表现与接受单一接种物的植物相似,而丛枝菌根真菌对B5植物的根定殖高于其他处理中的植物。结论总体而言,B5是最常与植物生物量增加相关的接种剂。这项研究强调了在评估MI时需要考虑微生物和环境背景。
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引用次数: 0
Impacts of shelterbelt systems on pasture production and soil bacterial and fungal communities in agricultural fields 防护林系统对牧场生产和农田土壤细菌真菌群落的影响
Pub Date : 2023-07-10 DOI: 10.1002/sae2.12059
Thi Bao-Anh Nguyen, Lina A. Henao, Zelin Li, Long Cheng, Hang-Wei Hu

Introduction

Shelterbelts, which provide protection for livestock and crops against severe weather conditions, have been recognised as important contributors to increased crop yields. Soil microorganisms play important roles in nutrient cycling, soil health and plant performance, thereby exerting significant influence on ecological services in terrestrial ecosystems. However, impacts of shelterbelts on soil microbial communities in pasture ecosystems remain poorly studied.

Materials and Methods

Here, we assessed the effect of shelterbelts (composed of Eucalyptus and native shrubs) on the pasture and nutrient productivity as well as soil bacterial and fungal communities at four distances from the shelterbelts with two different heights in two fields during spring and summer by applying amplicon sequencing, physicochemical and nutritional value analyses.

Results

The results showed that the shelterbelt height, distances from shelterbelts, seasons and their interactive effects substantially affected pasture yield, neutral detergent fibre, metabolisable energy and crude protein yields, but did not increase the pasture productivity in the rows adjacent to the shelterbelts. We also identified that the alpha diversity of bacteria and fungi did not significantly change across distances from the shelterbelts in both fields, but the community structure of soil bacteria and fungi was significantly influenced by distances from the shelterbelts. Interestingly, soil calcium (Ca) and shelterbelts were identified as top predictors of fungal community while soil pH and Ca was key drivers of bacterial community.

Conclusion

Our study provides novel knowledge in the impact of shelterbelts on pasture and nutritional production and soil microbial communities, which contributes to appropriate application and strategic management of the windbreak systems in agriculture production.

引言防护林为牲畜和作物提供保护,使其免受恶劣天气条件的影响,已被认为是提高作物产量的重要因素。土壤微生物在养分循环、土壤健康和植物生长性能中发挥着重要作用,从而对陆地生态系统的生态服务产生重大影响。然而,防护林对牧场生态系统中土壤微生物群落的影响研究仍然很少。材料与方法在这里,我们通过应用扩增子测序,评估了春季和夏季两个不同高度的防护林(由桉树和本地灌木组成)对牧场和养分生产力以及土壤细菌和真菌群落的影响,理化和营养价值分析。结果防护林高度、距防护林距离、季节及其交互作用对牧草产量、中性洗涤纤维、代谢能和粗蛋白产量有显著影响,但对防护林相邻行的牧草生产率没有提高。我们还发现,在两个田地中,细菌和真菌的α多样性在距离防护林的距离上没有显著变化,但土壤细菌和真菌群落结构受到距离防护林距离的显著影响。有趣的是,土壤钙(Ca)和防护林被确定为真菌群落的首要预测因子,而土壤pH和Ca是细菌群落的关键驱动因素。结论我们的研究为防护林对牧场和营养生产以及土壤微生物群落的影响提供了新的知识,有助于防风林系统在农业生产中的适当应用和战略管理。
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引用次数: 0
Organic management and soil health promote nutrient use efficiency 有机管理和土壤健康促进养分利用效率
Pub Date : 2023-07-05 DOI: 10.1002/sae2.12058
Misato Toda, Florian Walder, Marcel G. A. van der Heijden

Introduction

Nitrogen is a key nutrient for plants. Often less than 50% of the applied nitrogen fertilisers is acquired by crops and nitrogen can be easily lost into the environment causing environmental pollution. Thus, to make agriculture more sustainable, it is important to investigate which factors determine nitrogen use efficiency (NUE). We investigated whether NUE was higher in organically managed soils compared to conventionally managed soils.

Materials and Methods

To test this, we carried out a pot experiment in a greenhouse using soils from 16 fields. The soils were collected from conventionally (eight fields) or organically managed fields (eight fields). In addition, plants received two different 15N enriched N sources (mineral 15N or an organic fertiliser source, namely 15N enriched plant litter). Plants were harvested at three time points, and growth and nitrogen uptake were assessed at each time point.

Results

NUE depended on management type and harvest time and the higher NUE of organically managed soils became more evident towards the second and third harvest. The average NUE at the end of the experiment was 93% and 55% for mineral fertiliser and litter application, respectively. This indicated that mineral fertilisers were immediately acquired by the plants, while nutrients in organic amendments had a lower availability and probably would be supplied later but steadier. Further, NUE was positively linked to microbial biomass, soil organic carbon content, and aggregate size, indicating that enhanced soil quality and soil health leads to a more efficient use of fertilisers.

Conclusion

Our results indicate that organic management and soil health promote a more efficient use of nutrients and contribute to a more sustainable agriculture.

氮是植物的主要营养成分。通常只有不到50%的氮肥是由作物获得的,氮很容易流失到环境中,造成环境污染。因此,为了使农业更加可持续,研究哪些因素决定了氮利用效率(NUE)是很重要的。我们调查了有机管理土壤的NUE是否高于传统管理土壤。材料和方法为了测试这一点,我们在温室里用16块田地的土壤进行了盆栽实验。土壤是从传统的(八块田地)或有机管理的田地(八块田野)收集的。此外,植物接受两种不同的富含15N的氮源(矿物15N或有机肥料源,即富含15N植物枯枝落叶)。在三个时间点收获植物,并在每个时间点评估生长和氮吸收。结果NUE随管理类型和收获时间的不同而不同,有机管理土壤的NUE越高,在第二次和第三次收获时越明显。试验结束时,施用矿物肥料和枯枝落叶的平均NUE分别为93%和55%。这表明,矿物肥料立即被植物获取,而有机改良剂中的营养素的可用性较低,可能会晚些供应,但更稳定。此外,NUE与微生物生物量、土壤有机碳含量和团聚体大小呈正相关,这表明土壤质量和土壤健康的提高可以更有效地使用化肥。结论有机管理和土壤健康促进了养分的高效利用,有助于农业的可持续发展。
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引用次数: 3
Seed biopriming at different concentrations to assess the effects of Cyanobacteria on germination and seedling performance of keystone arid species 不同浓度的种子生物鉴定,以评估蓝藻对关键干旱物种发芽和幼苗性能的影响
Pub Date : 2023-07-03 DOI: 10.1002/sae2.12049
Nathali M. Machado-de-Lima, James Charlesworth, Jana Stewart, Mark K. J. Ooi, Miriam Muñoz-Rojas

Introduction

Biocrust cyanobacteria have a large potential as biofertilizers for restoring degraded ecosystems because of their ability to improve soil nutrition and stabilisation, and to produce metabolites such as phytohormones to enhance plant growth. However, important aspects regarding the effects of cyanobacteria on native plants, such as metabolite production or concentration of inoculants, remain unknown. Here, we investigated the effects of different concentrations of cyanobacteria, on the germination and seedling growth of keystone plant species used in dryland restoration. We hypothesised that the studied inoculant would improve germination and seedling growth rates, with specific effects associated with the inoculant's concentration and metabolomic profiles.

Methods

We bioprimed seeds of four native plant species, using a cyanobacterial inoculant with different proportions of Nostoc and Leptolyngbya at two different concentrations. We recorded germination, measured seedling growth, and determined the corrected vigour for each treatment and species. Metabolites produced by the cyanobacterial inoculant were assessed to identify plant growth hormones potentially driving any effects.

Results

There was a clear positive effect on the total germination of Triodia epactia and Triodia wiseana, but negative impacts for Senna notabilis and Grevillea wickhamii. There were also positive effects on root growth, but only for T. epactia, with negative or neutral impacts on the root and shoot growth of other species tested. We detected phytohormones, salicylic acid and indole-3-acetic acid, that were produced by our cyanobacteria inoculant, which are strongly linked to positive effects in early plant growth stages, but also known to inhibit growth when in higher concentrations.

Conclusion

The positive effects of the biopriming protocol used are not uniform and highlight the need to improve our understanding of the effects provided both from different consortia and the concentrations applied when inoculating. There is a very high value in improving restoration outcomes for native vegetation communities in arid and semi-arid regions.

简介生物锈蓝细菌作为恢复退化生态系统的生物肥料有很大的潜力,因为它们能够改善土壤营养和稳定,并产生植物激素等代谢产物来促进植物生长。然而,有关蓝藻对本地植物影响的重要方面,如代谢产物的产生或接种物的浓度,仍然未知。在这里,我们研究了不同浓度的蓝藻对旱地恢复中使用的关键植物物种的发芽和幼苗生长的影响。我们假设所研究的接种物将提高发芽率和幼苗生长率,其具体效果与接种物的浓度和代谢组学特征有关。方法我们用不同比例的发菜和钩藤在两种不同浓度的蓝藻接种剂对四种本地植物的种子进行生物取样。我们记录了发芽情况,测量了幼苗的生长,并确定了每种处理和物种的校正活力。对蓝藻接种剂产生的代谢产物进行了评估,以确定可能产生任何影响的植物生长激素。结果对艾帕蒂亚和薇莎草的总发芽率有明显的正向影响,而对珙桐和薇甘菊则有负向影响。对根系生长也有积极影响,但仅对T.epactia,对其他受试物种的根系和地上部生长有负面或中性影响。我们检测到由我们的蓝藻接种剂产生的植物激素水杨酸和吲哚-3-乙酸,它们在植物生长早期与积极作用密切相关,但在较高浓度时也会抑制生长。结论所使用的生物筛选方案的积极效果并不一致,这突出表明需要提高我们对不同联合体提供的效果和接种时应用的浓度的理解。在改善干旱和半干旱地区原生植被群落的恢复成果方面具有非常高的价值。
{"title":"Seed biopriming at different concentrations to assess the effects of Cyanobacteria on germination and seedling performance of keystone arid species","authors":"Nathali M. Machado-de-Lima,&nbsp;James Charlesworth,&nbsp;Jana Stewart,&nbsp;Mark K. J. Ooi,&nbsp;Miriam Muñoz-Rojas","doi":"10.1002/sae2.12049","DOIUrl":"https://doi.org/10.1002/sae2.12049","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Biocrust cyanobacteria have a large potential as biofertilizers for restoring degraded ecosystems because of their ability to improve soil nutrition and stabilisation, and to produce metabolites such as phytohormones to enhance plant growth. However, important aspects regarding the effects of cyanobacteria on native plants, such as metabolite production or concentration of inoculants, remain unknown. Here, we investigated the effects of different concentrations of cyanobacteria, on the germination and seedling growth of keystone plant species used in dryland restoration. We hypothesised that the studied inoculant would improve germination and seedling growth rates, with specific effects associated with the inoculant's concentration and metabolomic profiles.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We bioprimed seeds of four native plant species, using a cyanobacterial inoculant with different proportions of <i>Nostoc</i> and <i>Leptolyngbya</i> at two different concentrations. We recorded germination, measured seedling growth, and determined the corrected vigour for each treatment and species. Metabolites produced by the cyanobacterial inoculant were assessed to identify plant growth hormones potentially driving any effects.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>There was a clear positive effect on the total germination of <i>Triodia epactia</i> and <i>Triodia wiseana</i>, but negative impacts for <i>Senna notabilis</i> and <i>Grevillea wickhamii</i>. There were also positive effects on root growth, but only for <i>T. epactia</i>, with negative or neutral impacts on the root and shoot growth of other species tested. We detected phytohormones, salicylic acid and indole-3-acetic acid, that were produced by our cyanobacteria inoculant, which are strongly linked to positive effects in early plant growth stages, but also known to inhibit growth when in higher concentrations.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The positive effects of the biopriming protocol used are not uniform and highlight the need to improve our understanding of the effects provided both from different consortia and the concentrations applied when inoculating. There is a very high value in improving restoration outcomes for native vegetation communities in arid and semi-arid regions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100834,"journal":{"name":"Journal of Sustainable Agriculture and Environment","volume":"2 3","pages":"266-275"},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.12049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50119147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biome regulates the effects of long-term grazing on soil microbial diversity Biome调节长期放牧对土壤微生物多样性的影响
Pub Date : 2023-06-30 DOI: 10.1002/sae2.12056
Xuan Zhao, Jinting Cai, Yueqing Song, Jushan Liu, Deli Wang, Ling Wang

Introduction

Livestock overgrazing represents one of the most destructive uses of land in terrestrial ecosystems and threatens biodiversity. However, understanding the effects of livestock overgrazing on below-ground soil microbial diversity is limited, despite soil microbes representing the majority of biodiversity and determining ecosystem functioning.

Materials and Methods

To investigate the effects of overgrazing on soil microbial richness, a long-term grazing exclusion experiment was conducted at six sites including three meadow steppes and three typical steppes in northern China.

Results

Our results revealed that overgrazing decreased bacterial and fungal richness across temperate steppes in northern China, and the biome could regulate the overgrazing effects, especially for fungal richness. Specifically, the negative effects of overgrazing on microbial richness were highly significant in typical steppes while not significant in meadow steppes that contained higher plant diversity and precipitation. Partial least-squares path model showed that overgrazing affected soil microbial richness in highly complex ways, and the affected pathways were different in meadow steppes and typical steppes. The direct negative effects of grazing and their indirect negative effects via soil properties resulted in a significant decrease in microbial richness in typical steppes. In meadow steppes, the indirect beneficial effects via plant attributes offset the direct negative effects of grazing. Consequently, the soil microbial community in meadow steppe resisted overgrazing disturbance.

Conclusion

Our study illuminates the complex and highly biome-dependent grazing effects and pathways on soil microbiota and indicates that meadow steppe may be more resistant or resilient to human disturbance than typical steppe. These findings suggest that different grasslands might be managed differently considering their intrinsic characteristics to help biodiversity conservation. Moreover, future research should focus on the underlying mechanisms of grazing effects on soil microbial richness. Besides grazing-induced plant and soil traits changes, other potential pathways could strongly influence soil microbial diversity.

引言畜牧业过度放牧是陆地生态系统中最具破坏性的土地利用之一,威胁着生物多样性。然而,尽管土壤微生物代表了生物多样性的大部分,并决定了生态系统的功能,但了解牲畜过度放牧对地下土壤微生物多样性的影响是有限的。材料与方法为了研究过度放牧对土壤微生物丰富度的影响,在中国北方三个草地草原和三个典型草原等六个地点进行了长期排牧试验。结果研究结果表明,过度放牧降低了中国北方温带草原的细菌和真菌丰富度,生物群落可以调节过度放牧效应,尤其是真菌丰富度。具体而言,过度放牧对微生物丰富度的负面影响在典型草原中非常显著,而在植物多样性和降水量较高的草甸草原中则不显著。偏最小二乘路径模型表明,过度放牧以高度复杂的方式影响土壤微生物丰富度,草地草原和典型草原的影响途径不同。放牧的直接负面影响及其通过土壤性质产生的间接负面影响导致典型草原微生物丰富度显著下降。在草地草原上,通过植物属性产生的间接有益影响抵消了放牧的直接负面影响。因此,草甸草原的土壤微生物群落能够抵抗过度放牧的干扰。结论我们的研究阐明了放牧对土壤微生物群的复杂且高度依赖生物群落的影响和途径,并表明草甸草原可能比典型草原更能抵抗或恢复人类干扰。这些发现表明,考虑到不同草原的内在特征,可能会对其进行不同的管理,以帮助保护生物多样性。此外,未来的研究应侧重于放牧对土壤微生物丰富度影响的潜在机制。除了放牧引起的植物和土壤性状变化外,其他潜在途径也会强烈影响土壤微生物多样性。
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引用次数: 0
Ancient and modern wheat varieties: A trade-off between soil CO2 emissions and grain yield? 古代和现代小麦品种:土壤二氧化碳排放和粮食产量之间的权衡?
Pub Date : 2023-06-29 DOI: 10.1002/sae2.12048
Laly Rouch, Stéphane Follain, Eric Pimet, Florian Bizouard, Elodie Cognard, Olivier Mathieu, Manuel Blouin

Introduction

Humanity is facing two great challenges: producing enough food for a growing population and mitigating greenhouse gas emissions. In this study, we investigated the choice of specific wheat varieties to improve carbon storage in soil while producing enough grain to assure food security. We hypothesize that ancient wheat varieties could store more carbon than modern ones, due to a likely bigger and deeper root system or to more recalcitrant root organic matter.

Materials and Methods

We conducted a field experiment with four modern and four ancient wheat varieties, on four different sites with contrasted soil properties. Root morphology was assessed by image analysis and potential CO2 emissions by incubation for 60 days. Since in situ carbon storage differences between ancient and modern varieties were expected to be weak and not cumulated due to rotation, we estimated expected CO2 emissions from root biomass and potential CO2 emissions. The grain yield was also measured.

Results

The breeding type (ancient vs. modern varieties) affected root length in two of our four sites, with longer roots for ancient varieties, but it did not affect other root traits such as biomass. The breeding type also affected CO2 emissions, with higher measured CO2 emissions for modern than ancient varieties in Arenic Cambisol conditions (Morvan), and higher estimated (considering root biomass variations) CO2 emissions for modern varieties in Rendzic Leptosol conditions (Saint Romain). Root traits and estimated CO2 emissions were also dependent on the soil properties of the different sites. We did not find any significant differences in grain yield between ancient and modern varieties.

Conclusion

A possible trade-off between carbon storage and grain production was expected, but our results suggest that some types of soil can support both high grain yield and C storage, especially those with an important depth, a neutral pH and a fine texture.

引言人类面临两大挑战:为不断增长的人口生产足够的粮食和减少温室气体排放。在这项研究中,我们调查了特定小麦品种的选择,以提高土壤中的碳储量,同时生产足够的粮食来确保粮食安全。我们假设,由于根系可能更大、更深,或者根系有机质更难分解,古代小麦品种可能比现代小麦品种储存更多的碳。材料和方法我们在四个不同的地点对四个现代和四个古代小麦品种进行了田间试验,并对土壤性质进行了对比。通过图像分析评估根形态,并通过培养60天评估潜在的CO2排放。由于古代和现代品种之间的原位碳储存差异预计很小,不会因轮作而累积,我们估计了根系生物量的预期二氧化碳排放量和潜在二氧化碳排放量。还测定了粮食产量。结果育种类型(古代品种与现代品种)影响了我们四个地点中的两个地点的根长,古代品种的根较长,但不影响其他根系性状,如生物量。育种类型也影响CO2排放,在Arenic Cambisol条件下(Morvan),现代品种的测量CO2排放量高于古代品种,在Rendzic Leptosol条件下(Saint-Romain),现代变种的估计CO2排放量(考虑到根系生物量的变化)更高。根系特征和估计的二氧化碳排放量也取决于不同地点的土壤特性。我们没有发现古代和现代品种之间的粮食产量有任何显著差异。结论碳储存和粮食生产之间可能存在权衡,但我们的研究结果表明,某些类型的土壤既能支持粮食高产,又能支持碳储存,尤其是那些深度重要、pH值中性、质地细腻的土壤。
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引用次数: 0
Effects of biostimulant application on soil biological and physicochemical properties: A field study 施用生物刺激剂对土壤生物和理化性质的影响:田间研究
Pub Date : 2023-06-19 DOI: 10.1002/sae2.12057
Jenendra Wadduwage, Hongwei Liu, Eleonora Egidi, Brajesh K. Singh, Catriona A. Macdonald

Introduction

Despite their potential benefits, it is not well understood how the application of biostimulants influences soil biological properties and their microbial communities in field conditions. In this study, we aimed to evaluate the impacts of biostimulants on soil biological and physicochemical properties relevant to soil health.

Materials and Methods

To achieve this, we conducted a field study to investigate the effects of two types of commercially available biostimulants, Universal Natural Plant food (UNP) and Converte Seed Primer (CSP), on microbial activity, bacterial and fungal abundance, community structure and diversity, and soil chemical and physical properties across two depths (0–10 and 10–20 cm) from five sites under either wheat or pasture cultivation.

Results

Our findings suggest that application of UNP stimulated microbial activity by 40.1% in surface (0–10 cm) and 36.4% in deeper (10–20 cm) soil, but was dependant on site. Effects were generally greater in grasslands compared with arable soils. At sites where UNP stimulated microbial respiration, substrate-induced respiration was also stimulated in surface soils and was associated with increased soil moisture content and higher total carbon and nitrogen. At the one site where UNP was combined with CSP, soil enzymes associated with carbon and nitrogen cycling were stimulated in UNP and UNP + CSP treatments. Total bacterial and fungal abundance and their alpha diversity did not respond to biostimulant treatment. However, microbial indicator communities were identified that responded positively to UNP and CSP addition across the two depths. Bacterial indicator species included Elsterales, Propionibacteriales, Solibacterales, Candatus, Reyranellales and Sphingomonadales, but differed between depths. For the fungal indicator species Filobasidiales (Basidiomycota) and Pleosporales (Ascomycota) were strong responders and common across both depths.

Conclusion

Overall, our results suggest some positive effects of biostimulants on soil biological and physicochemical properties. Further long-term studies should be conducted to evaluate the effects of biostimulants on crop yield and farm resilience.

引言尽管生物刺激剂有潜在的好处,但人们还不清楚生物刺激剂的应用如何影响土壤生物特性及其在田间条件下的微生物群落。在本研究中,我们旨在评估生物刺激剂对与土壤健康相关的土壤生物和理化性质的影响。材料和方法为了实现这一点,我们进行了一项实地研究,研究了两种商业可用的生物刺激剂,通用天然植物食品(UNP)和转化种子引物(CSP)对微生物活性、细菌和真菌丰度、群落结构和多样性的影响,以及两个深度(0-10和10-20)的土壤化学和物理特性 cm)。结果我们的研究结果表明,UNP的应用刺激了表面40.1%的微生物活性(0-10 cm)和36.4%(10-20 cm)土壤,但取决于现场。与耕地相比,草原的影响通常更大。在UNP刺激微生物呼吸的地点,基质诱导的呼吸在表层土壤中也受到刺激,并与土壤含水量增加和总碳氮增加有关。在UNP与CSP结合的一个位点,与碳和氮循环相关的土壤酶在UNP和UNP中受到刺激 + CSP处理。细菌和真菌的总丰度及其α多样性对生物刺激剂处理没有反应。然而,在两个深度发现了对UNP和CSP添加有积极反应的微生物指示群落。细菌指示种包括Elsterales、丙酸杆菌目、Solibacteriales、Candatus、Reyranellales和Sphingomodales,但深度不同。对于真菌指示物种Filobasidiales(担子菌门)和Pleosporales(子囊菌门),反应强烈,在两个深度都很常见。结论总的来说,我们的研究结果表明,生物刺激剂对土壤的生物和理化性质有一些积极的影响。应进行进一步的长期研究,以评估生物刺激剂对作物产量和农场恢复力的影响。
{"title":"Effects of biostimulant application on soil biological and physicochemical properties: A field study","authors":"Jenendra Wadduwage,&nbsp;Hongwei Liu,&nbsp;Eleonora Egidi,&nbsp;Brajesh K. Singh,&nbsp;Catriona A. Macdonald","doi":"10.1002/sae2.12057","DOIUrl":"https://doi.org/10.1002/sae2.12057","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Despite their potential benefits, it is not well understood how the application of biostimulants influences soil biological properties and their microbial communities in field conditions. In this study, we aimed to evaluate the impacts of biostimulants on soil biological and physicochemical properties relevant to soil health.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>To achieve this, we conducted a field study to investigate the effects of two types of commercially available biostimulants, Universal Natural Plant food (UNP) and Converte Seed Primer (CSP), on microbial activity, bacterial and fungal abundance, community structure and diversity, and soil chemical and physical properties across two depths (0–10 and 10–20 cm) from five sites under either wheat or pasture cultivation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Our findings suggest that application of UNP stimulated microbial activity by 40.1% in surface (0–10 cm) and 36.4% in deeper (10–20 cm) soil, but was dependant on site. Effects were generally greater in grasslands compared with arable soils. At sites where UNP stimulated microbial respiration, substrate-induced respiration was also stimulated in surface soils and was associated with increased soil moisture content and higher total carbon and nitrogen. At the one site where UNP was combined with CSP, soil enzymes associated with carbon and nitrogen cycling were stimulated in UNP and UNP + CSP treatments. Total bacterial and fungal abundance and their alpha diversity did not respond to biostimulant treatment. However, microbial indicator communities were identified that responded positively to UNP and CSP addition across the two depths. Bacterial indicator species included <i>Elsterales, Propionibacteriales, Solibacterales, Candatus, Reyranellales</i> and <i>Sphingomonadales</i>, but differed between depths. For the fungal indicator species <i>Filobasidiales</i> (Basidiomycota) and <i>Pleosporales</i> (Ascomycota) were strong responders and common across both depths.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Overall, our results suggest some positive effects of biostimulants on soil biological and physicochemical properties. Further long-term studies should be conducted to evaluate the effects of biostimulants on crop yield and farm resilience.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100834,"journal":{"name":"Journal of Sustainable Agriculture and Environment","volume":"2 3","pages":"285-300"},"PeriodicalIF":0.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.12057","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50152424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Predicting soil fungal communities from chemical and physical properties 从化学和物理性质预测土壤真菌群落
Pub Date : 2023-06-12 DOI: 10.1002/sae2.12055
Natacha Bodenhausen, Julia Hess, Alain Valzano, Gabriel Deslandes-Hérold, Jan Waelchli, Reinhard Furrer, Marcel G. A. van der Heijden, Klaus Schlaeppi

Introduction

Biogeography describes spatial patterns of diversity and explains why organisms occur in given conditions. While it is well established that the diversity of soil microbes is largely controlled by edaphic environmental variables, microbiome community prediction from soil properties has received less attention. In this study, we specifically investigated whether it is possible to predict the composition of soil fungal communities based on physicochemical soil data using multivariate ordination.

Materials and Methods

We sampled soil from 59 arable fields in Switzerland and assembled paired data of physicochemical soil properties as well as profiles of soil fungal communities. Fungal communities were characterized using long-read sequencing of the entire ribosomal internal transcribed spacer. We used redundancy analysis to combine the physical and chemical soil measurements with the fungal community data.

Results

We identified a reduced set of 10 soil properties that explained fungal community composition. Soil properties with the strongest impact on the fungal community included pH, potassium and sand content. Finally, we evaluated the model for its suitability for prediction using leave-one-out validation. The prediction of community composition was successful for most soils, and only 3/59 soils could not be well predicted (Pearson correlation coefficients between observed and predicted communities of <0.5). Further, we successfully validated our prediction approach with a publicly available data set. With both data sets, prediction was less successful for soils characterized by very unique properties or diverging fungal communities, while it was successful for soils with similar characteristics and microbiome.

Conclusions

Reliable prediction of microbial communities from chemical soil properties could bypass the complex and laborious sequencing-based generation of microbiota data, thereby making soil microbiome information available for agricultural purposes such as pathogen monitoring, field inoculation or yield projections.

引言生物地理学描述了多样性的空间模式,并解释了生物体在特定条件下发生的原因。虽然土壤微生物的多样性在很大程度上受到土壤环境变量的控制,但从土壤性质预测微生物组群落的关注较少。在这项研究中,我们特别研究了是否有可能使用多元排序法基于理化土壤数据预测土壤真菌群落的组成。材料与方法我们对瑞士59块耕地的土壤进行了采样,收集了土壤理化性质和土壤真菌群落特征的配对数据。使用整个核糖体内部转录间隔区的长读测序来表征真菌群落。我们使用冗余分析将物理和化学土壤测量与真菌群落数据相结合。结果我们确定了一组简化的10种土壤特性,可以解释真菌群落组成。对真菌群落影响最大的土壤性质包括pH、钾和沙子含量。最后,我们使用留一验证来评估该模型的预测适用性。大多数土壤的群落组成预测是成功的,只有3/59的土壤不能很好地预测(观测到的和预测到的群落之间的Pearson相关系数<;0.5)。此外,我们用公开的数据集成功地验证了我们的预测方法。对于这两个数据集,预测对具有非常独特特性或真菌群落分化特征的土壤不太成功,而对具有相似特性和微生物组的土壤则很成功。结论从土壤化学性质可靠地预测微生物群落可以绕过复杂而费力的基于测序的微生物群数据生成,从而使土壤微生物组信息可用于农业目的,如病原体监测、田间接种或产量预测。
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引用次数: 3
Climate and environmental data contribute to the prediction of grain commodity prices using deep learning 气候和环境数据有助于利用深度学习预测粮食商品价格
Pub Date : 2023-06-06 DOI: 10.1002/sae2.12041
Zilin Wang, Niamh French, Thomas James, Calogero Schillaci, Faith Chan, Meili Feng, Aldo Lipani

Background

Grain commodities are important to people's daily lives and their fluctuations can cause instability for households. Accurate prediction of grain prices can improve food and social security.

Methods & Materials

This study proposes a hybrid Long Short-Term Memory (LSTM)-Convolutional Neural Network (CNN) model to forecast weekly oat, corn, soybean and wheat prices in the United States market. The LSTM-CNN is a multivariate model that uses weather data, macroeconomic data, commodities grain prices and snow factors, including Snow Water Equivalent (SWE), snowfall and snow depth, to make multistep ahead forecasts.

Results

Of all the features, the snow factor is used for the first time for commodity price forecasting. We used the LSTM-CNN model to evaluate the 5, 10, 15 and 20 weeks ahead forecasting and this hybrid model had the lowest Mean Squared Error (MSE) at 5, 10 and 15 weeks ahead of prediction. In addition, Shapley values were calculated to analyse the feature contribution of the LSTM-CNN model when forecasting the testing set. Based on the feature contribution, SWE ranked third, fifth and seventh in feature importance in the 5-week ahead forecast for corn, oats and wheat, respectively, and 7–8 places higher than total precipitation, indicating the potential use of SWE in grain price forecasting.

Conclusion

The hybrid multivariate LSTM-CNN model outperformed other models and the newly involved climate data, SWE, showed the research potential of using snow as an input variable to predict grain prices over a multistep ahead time horizon.

背景粮食商品对人们的日常生活很重要,其波动可能会导致家庭不稳定。准确预测粮食价格可以改善粮食和社会保障。方法&;材料本研究提出了一种长短期记忆(LSTM)-卷积神经网络(CNN)混合模型,用于预测美国市场每周燕麦、玉米、大豆和小麦的价格。LSTM-CNN是一个多变量模型,使用天气数据、宏观经济数据、商品粮食价格和雪因素,包括雪水当量(SWE)、降雪量和雪深,进行多步预测。结果在所有特征中,首次将雪因子用于商品价格预测。我们使用LSTM-CNN模型来评估5、10、15和20周前的预测,并且该混合模型在预测前5、10和15周的均方误差(MSE)最低。此外,在预测测试集时,还计算了Shapley值来分析LSTM-CNN模型的特征贡献。基于特征贡献,在玉米、燕麦和小麦的5周预测中,SWE的特征重要性分别排名第三、第五和第七,比总降水量高7-8位,表明SWE在粮食价格预测中的潜在用途。结论混合多元LSTM-CNN模型优于其他模型,新涉及的气候数据SWE显示了将雪作为输入变量在多步时间范围内预测粮食价格的研究潜力。
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引用次数: 0
期刊
Journal of Sustainable Agriculture and Environment
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