Pharmacological Research - Modern Chinese Medicine最新文献

{"title":"The effect of topically applied Hesperidin on Imiquimod-induced Psoriasis in a mouse model","authors":"Doaa Muwafaq Nassrullah,&nbsp;Huda I. Al Qadhi","doi":"10.1016/j.prmcm.2025.100745","DOIUrl":"10.1016/j.prmcm.2025.100745","url":null,"abstract":"<div><h3>Introduction</h3><div>Psoriasis is a long-lasting, inflammatory autoimmune condition that primarily affects the skin and those with a significant genetic susceptibility. Hesperidin is a polyphenolic bioflavonoid belonging to the flavanone glycoside class that exhibits antioxidant and anti-inflammatory actions. Hesperidin is found in many herbs used in traditional Chinese medicine (TCM), especially Zhiqiao (<strong>枳壳</strong>, Zhỹ Qiào; dried Citrus aurantium peel) and Chenpi (<strong>陳皮</strong>, Chén Pí; dried Citrus reticulata peel).</div></div><div><h3>Methods</h3><div>A total of 48 albino mice were divided into 6 groups, each of 8 mice. The effects of clinical observation, histopathological examination, and biomarker analysis were evaluated.</div></div><div><h3>Results</h3><div>Hesperidin and its combination with clobetasol significantly reduced imiquimod-induced elevations in the Psoriasis Area and Severity Index (PASI) and Baker’s scores (P&lt;0.001), also significantly diminished inflammatory markers, including tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-17A (IL-17A) as well as oxidative markers malondialdehyde (MDA) (P&lt;0.001), while increasing superoxide dismutase (SOD) levels (P&lt;0.001).</div></div><div><h3>Conclusion</h3><div>Topical administration of hesperidin may be a promising agent for psoriasis treatment alone or in combination with clobetasol. This is due to the significant anti-inflammatory and antioxidant effects<strong>.</strong> The study's findings align with the traditional use of hesperidin-rich TCM herbs, including Zhiqiao and Chenpi.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"18 ","pages":"Article 100745"},"PeriodicalIF":0.0,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146090242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review of the bioactive compounds and the various pharmacological activities of Mahua (Madhuca longifolia) plant","authors":"Sabina Yasmin ,&nbsp;Rasmita Nayak ,&nbsp;Fatima M Al-Salam ,&nbsp;Anupama Diwan ,&nbsp;Rani Mansuri ,&nbsp;Sumel Ashique ,&nbsp;Md Yousuf Ansari","doi":"10.1016/j.prmcm.2025.100746","DOIUrl":"10.1016/j.prmcm.2025.100746","url":null,"abstract":"<div><h3>Introduction</h3><div>Mahua (<em>Madhuca longifolia</em>) is a tree commonly found in tropical regions (family: Sapotaceae). The main phytoconstituents present in flowers and other parts of the plant (known as sweet flower) have a wide range of Traditional Chinese Medicine (TCM) and Ayurvedic applications in the treatment of various disease conditions, including hepatoprotective, anti-inflammatory, and antihyperglycemic activities, especially for kidney stones. Recently, research publications have highlighted greater concern about the application of bioactive constituents and the identification of genes related to stress tolerance, which may help breed or engineer <em>M. longifolia</em> varieties with enhanced medicinal compound production, supporting consistent quality and efficacy in herbal products. The review further highlights the phytochemical composition of mahua, its pharmacological actions in treating certain diseases, and its full mechanism of action, including its pharmacological activities in treating various diseases and ailments.</div></div><div><h3>Methodology</h3><div>The main study aims to collect all data and information that are collected from previously published reports that are related to “<em>Madhuca longifolia</em>”, “Pharmacological activities of mahua”“, traditional chinese medicine” and their constituents, “mahua phytoconstituents”, which were retrieved from different databases (Scopus, Google Scholar, and PubMed). Hence, ethical authorisation is not required. We retrieved, finalised, and utilised a comprehensive review of 81 articles from 2000 to 2025.</div></div><div><h3>Results</h3><div>Data from different sources have been collected, and each research publication related to our objective is being analysed. This review further highlights the phytochemical composition of other parts of the mahua plant and the reported pharmacological activities. This review suggested that its various applications warrant further research and investigation.</div></div><div><h3>Conclusion</h3><div>Mahua’s flower is used as an ayurvedic formulation, and its extracts have potential for the treatment of various disease conditions and also boost immunity.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"18 ","pages":"Article 100746"},"PeriodicalIF":0.0,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulatory Role of Traditional Chinese Medicine in Gut–Liver and Adipose–Liver Axis Dysfunction in MASLD","authors":"Sampat Singh Tanwar,&nbsp;Seema Sharma","doi":"10.1016/j.prmcm.2025.100743","DOIUrl":"10.1016/j.prmcm.2025.100743","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Introduction&lt;/h3&gt;&lt;div&gt;Metabolic dysfunction-Associated Steatotic Liver Disease (MASLD) is the most common chronic liver disease worldwide, affecting around 24% of the population, with regional prevalence ranging from 31.8% in the Middle East to 13.5% in Africa (WHO). MASLD encompasses a spectrum from simple steatosis to non-alcoholic steatohepatitis (NASH), fibrosis, cirrhosis, and hepatocellular carcinoma. Its complex pathogenesis involves insulin resistance, obesity, genetic factors, and chronic inflammation, leading to excessive lipid accumulation in hepatocytes. Current Western treatments primarily address symptoms but offer limited efficacy. Traditional Chinese Medicine (TCM) provides a holistic approach focused on restoring Yin-Yang balance and Qi flow, viewing liver dysfunction as related to dampness and Qi stagnation. Modern research supports TCM’s multi-target actions, showing herbal medicines improve gut microbiota, strengthen the intestinal barrier, reduce inflammation, and modulate adipokines and insulin sensitivity. These mechanisms support TCM’s potential systemic efficacy in managing MASLD, integrating traditional theory with modern pharmacology.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;A systematic literature search was conducted across major databases including PubMed, Scopus, and Web of Science to identify relevant preclinical and clinical studies published up to 2025. The search strategy employed combinations of keywords such as “Metabolic dysfunction-Associated Steatotic Liver Disease (MASLD),” “Traditional Chinese Medicine (TCM),” “gut-liver axis,” “adipose-liver axis,” “herbal medicine,” “gut microbiota,” and “adipokines.” Studies were included if they investigated molecular mechanisms or therapeutic effects of Chinese herbal medicines in MASLD models, particularly focusing on modulation of the gut-liver and adipose tissue-liver axes. Both in vitro and in vivo experimental studies, as well as relevant clinical trials, were considered. Studies were excluded if they were non-English, case reports, reviews without original data, or lacked mechanistic or therapeutic relevance. This approach aimed to ensure a comprehensive and focused synthesis of high-quality evidence supporting the systemic effects of TCM in MASLD.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;Recent studies highlight the gut-liver and adipose-liver axes as key contributors to MASLD progression. Gut dysbiosis alters bile acids, tryptophan catabolites, and BCAAs, where &lt;em&gt;Bacteroides&lt;/em&gt; species promote secondary bile acid accumulation, disrupting FXR signaling and causing lipid imbalance and inflammation. Tryptophan-derived indoles impair gut barrier integrity, worsening liver inflammation and fibrosis, while elevated BCAAs associate with insulin resistance and hepatocyte damage. Obesity-induced adipose dysfunction drives MASLD by releasing excess FFAs, activating NF-κB and PPAR-γ pathways, and secreting pro-inflammatory cytokines (TNF-α, IL-6), further impairing","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"18 ","pages":"Article 100743"},"PeriodicalIF":0.0,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145884631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative multi-omics analysis to decipher the mechanism by which the interaction between Danggui Buxue Tang and gut microbiota drives isoflavone metabolic transformation","authors":"Yutong Qi ,&nbsp;Zixia Chen ,&nbsp;Jiantang Zhang ,&nbsp;Ruilan Du ,&nbsp;Jingwen Shi ,&nbsp;Qizhu Chen ,&nbsp;Jun Chen ,&nbsp;Huaben Bo","doi":"10.1016/j.prmcm.2025.100744","DOIUrl":"10.1016/j.prmcm.2025.100744","url":null,"abstract":"<div><h3>Introduction</h3><div>Danggui Buxue Tang (DBT) is a classic herbal formula traditionally used to \"tonify Qi and generate blood.\" Its efficacy is believed to depend on the biotransformation of its bioactive compounds by the gut microbiota; however, the specific metabolic profile and the key bacterial taxa involved remain unclear.</div></div><div><h3>Methods</h3><div>An in vitro anaerobic fermentation model was established to simulate gut microbial biotransformation of DBT. This model allowed for a controlled and reproducible investigation of DBT-microbiota interactions. We integrated untargeted metabolomics (LC-MS) with 16S rRNA gene sequencing to analyze the concomitant changes in metabolites and the microbial community. PICRUSt2 was used for functional prediction. Additionally, network pharmacology and molecular docking were employed to construct a \"microbiota-enzyme-metabolite-target\" network.</div></div><div><h3>Results</h3><div>Untargeted metabolomics analysis identified 734 significantly altered metabolites following DBT fermentation, including notable increases in formononetin, biochanin A, and xenognosin B. 16S rRNA gene sequencing revealed that DBT markedly increased the relative abundance of Bifidobacterium (from 0.05 % to 25.5 %) while suppressing Porphyromonas. Functional prediction indicated significant enrichment in the \"Drug metabolism - other enzymes\" pathway. Correlation analysis revealed significant associations between specific gut microbiota and metabolites. A three-step metabolic cascade (glycoside hydrolysis → methylation → hydroxylation) was identified, which reduced toxicity by 90 % and increased clearance twofold. The metabolites exhibited strong binding to targets such as CDK2 (binding energy ≤ -7.24 kcal/mol) and were enriched in hematopoiesis-related pathways, including the PI3K-Akt signaling pathway.</div></div><div><h3>Discussion</h3><div>This study systematically elucidates how the gut microbiota transforms DBT isoflavones into active aglycones that target core hematopoietic proteins, providing a novel perspective on the mechanism of orally administered, poorly absorbable traditional Chinese medicine formulas. Our results confirm that the gut microbiota is indispensable for the efficacy of DBT.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"18 ","pages":"Article 100744"},"PeriodicalIF":0.0,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145840926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing cordycepin’s therapeutic potential: A review with special focus on breast cancer","authors":"Shanmugham Poongkuzhali,&nbsp;Natarajan Muninathan,&nbsp;Arumugam Suresh,&nbsp;Christina Beula","doi":"10.1016/j.prmcm.2025.100742","DOIUrl":"10.1016/j.prmcm.2025.100742","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Introduction&lt;/h3&gt;&lt;div&gt;Cordycepin (CD), 3′- deoxy adenosine, is a major bioactive compound secreted by the entomopathogenic fungus &lt;em&gt;Cordyceps militaris&lt;/em&gt; and &lt;em&gt;Cordyceps sinensis. Cordyceps sinensis&lt;/em&gt; is known as &lt;em&gt;DongChongXiaCa&lt;/em&gt; in Traditional Chinese Medicine (TCM). It possesses various pharmacological properties such as anti-inflammatory, immunomodulatory, anti-tumor activities. It also alleviates fatigue. Its therapeutic potential extends from cancer to several metabolic disorders. The therapeutic efficacy of CD in preclinical breast cancer (BC) studies has been highlighted with a focus on molecular pathways modulated by the compound.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;A systematic literature search was conducted across various electronic databases such as Google Scholar and PubMed in order to identify the relevant &lt;em&gt;in vitro&lt;/em&gt; and &lt;em&gt;in vivo&lt;/em&gt; studies. The chosen articles focused on the molecular mechanisms modulated by CD in BC and in other cancers. Articles related to therapeutic effects of CD against metabolic disorders, synergistic mechanisms with conventional treatments, bioavailability and pharmacokinetic limitations, clinical prospects and challenges of CD were also retrieved.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;Pre-clinical studies reported that CD possesses broad spectrum of biological properties, such as anti-tumor, anti-aging, anti-hyperlipidemic, inhibition of fat accumulation, reduction of body weight, anti-viral, anti-SARS-CoV-2, anti-hyperglycemic and skin-lightening properties&lt;em&gt;. In vitro&lt;/em&gt; and &lt;em&gt;in vivo&lt;/em&gt; studies highlighted the anti-cancer property of CD against BC. It covers the molecular pathways modulated by the natural compound in BC. CD induced apoptosis by upregulating Bax/BcL ratio, cleaved caspases 8 and 7 and downregulating BcL-2 in BC cell lines as well as in Triple Negative Breast Cancer (TNBC) xenograft models. It initiated autophagy in MCF-7 cells and apoptosis in MDA-MB-231 cells. CD inhibited cell invasion and metastasis by downregulating matrix metalloproteinases (MMP’s), epithelial-mesenchymal transition (EMT) markers in TNBC xenograft model. It also downregulated EMT transcription factors in TNBC cancer cell lines, inhibited markers of hedgehog signaling pathway. Several other BC molecular pathways regulated by CD are also covered. Apart from anti-BC activity, it also possesses anti-hyperlipidemic, anti-hyperglycemic, immunomodulatory, anti-obesity, anti-pigmentation, anti-anxiety, stress lowering, and anti-hypertensive properties. Pre-clinical studies on CD’s anti-cancer activity against colon, hepatocellular, leukemia and other cancers have been included. It displayed synergistic effects when combined with conventional chemotherapeutic drugs, including doxorubicin, cisplatin, gemcitabine and apatinib. One of the major limitations of CD is the bioavailability of the compound inside the human body. As CD is susceptible to degradation by adenosine deaminase (A","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"18 ","pages":"Article 100742"},"PeriodicalIF":0.0,"publicationDate":"2025-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145840925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reframing antimicrobial synergy through traditional and translational lenses","authors":"Md. Sanower Hossain","doi":"10.1016/j.prmcm.2025.100739","DOIUrl":"10.1016/j.prmcm.2025.100739","url":null,"abstract":"","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"18 ","pages":"Article 100739"},"PeriodicalIF":0.0,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145750390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quail egg yolk oil attenuates insulin resistance and redox imbalance in a high-sugar-diet drosophila model","authors":"Muhammad Sani Ismaila ,&nbsp;Ismail Sulaiman ,&nbsp;Abdulbariu Ogirima Uhuami ,&nbsp;Venkatesan Sundaram ,&nbsp;Mustapha Umar Imam","doi":"10.1016/j.prmcm.2025.100740","DOIUrl":"10.1016/j.prmcm.2025.100740","url":null,"abstract":"<div><h3>Introduction</h3><div>Type 2 diabetes mellitus (T2DM) is increasing globally, creating a need for natural and functional food–based therapeutic interventions. Quail egg yolk oil (QEYO), traditionally used in Chinese medicine as 鹌鹑蛋黄油 (ānchún dànhuáng yóu) for nourishing Yin and supporting metabolic balance, contains bioactive lipids and antioxidants with potential relevance for glycemic and redox regulation. This study investigated the antidiabetic and antioxidant effects of QEYO in a high-sugar diet (HSD)–induced metabolic dysfunction model in <em>Drosophila melanogaster</em>.</div></div><div><h3>Methods</h3><div>Adult male <em>Drosophila melanogaster</em> were fed a high-sugar diet (30% sucrose) to induce metabolic dysfunction and allocated into five groups: control, HSD, QEYO (62.5 or 125 mg/10 mL diet), and metformin (16 mg/10 mL). After 14 days of HSD induction followed by 7 days of treatment, physiological parameters, glucose and lipid biomarkers, oxidative stress indicators, and expression of key metabolic genes were assessed using enzymatic assays and RT-qPCR.</div></div><div><h3>Results</h3><div>HSD-fed flies developed obesity, hyperglycemia, elevated triglycerides, oxidative stress, and dysregulation of insulin pathway–related genes. QEYO significantly ameliorated these abnormalities in a dose-dependent manner. The 62.5 mg dose produced stronger glucose-lowering effects, while the 125 mg dose showed greater antioxidant enhancement and improved expression of SOD, CAT, IRS, and DILP2. Metformin produced comparable metabolic benefits.</div></div><div><h3>Discussion</h3><div>QEYO improved glucose homeostasis, lipid metabolism, and insulin pathway activity, and reduced markers of insulin resistance and oxidative imbalance in HSD-induced flies. These findings support its potential as a functional food–based intervention for T2DM and correspond with its traditional role in Chinese dietary therapy for metabolic regulation. Further mammalian studies are required for translational confirmation.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"18 ","pages":"Article 100740"},"PeriodicalIF":0.0,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145750151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aganope thyrsiflora (Benth.) Polhill: Acute, sub-acute, and in silico toxicological evaluation of an underexplored ethnomedicinal plant","authors":"Lalnun Hruaitluangi ,&nbsp;Lalduhawma Chhakchhuak ,&nbsp;Anima Debbarma ,&nbsp;C. Malsawmtluangi ,&nbsp;Hauzel Lalhlenmawia ,&nbsp;Ajmal Koya Pulikkal ,&nbsp;Lalzikpuii Sailo","doi":"10.1016/j.prmcm.2025.100735","DOIUrl":"10.1016/j.prmcm.2025.100735","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div><em>Aganope thyrsiflora</em> (Benth.) Polhill, is a lesser-known ethnomedicinal plant traditionally used to treat gastrointestinal ailments. Despite its use in folk medicine, its pharmacological efficacy and safety profile remain largely unexamined.</div></div><div><h3>Aim of the study</h3><div>To evaluate the systemic effects and potential toxicological risks of the methanolic extract of <em>A. thyrsiflora</em> through combined experimental and computational approaches.</div></div><div><h3>Materials and methods</h3><div><em>In vivo</em> acute and sub-acute toxicity were assessed via biochemical analysis, liver histopathology, and serum electrolyte analysis. LC-MS identified major phytoconstituents, which were further evaluated using <em>in silico</em> toxicity prediction tools.</div></div><div><h3>Results</h3><div>Extract treatment increased serum cholesterol, triglycerides, and very low-density lipoproteins (VLDL), while reducing low-density lipoprotein (LDL) levels. Liver function markers remained within physiological ranges, though serum alkaline phosphatase (SAP) increased at higher doses. Histopathology confirmed preserved hepatic structure without pathological changes. A remarkable hypoglycemic effect were observed. LC-MS identified fifteen major compounds, several of which were flagged with organ-specific toxicity alerts <em>in silico</em>.</div></div><div><h3>Conclusion</h3><div>The methanolic extract of <em>A. thyrsiflora</em> shows hypoglycemic potential and general hepatic safety at tested doses. However, its metabolic effects and predicted compound-specific toxicities highlight the need for further investigation. This study provides the first toxicological basis for future pharmacological validation of this underexplored ethnomedicinal species.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"18 ","pages":"Article 100735"},"PeriodicalIF":0.0,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo, in vitro, and in silico evaluation of the analgesic, antidiarrheal, and anthelmintic activities of methanolic extract of Syzygium grande (Wight) Walp","authors":"Md. Jahirul Islam Mamun,&nbsp;Md. Hossain Rasel,&nbsp;Zobayed Islam,&nbsp;Khurshida Jahan Suma,&nbsp;Mohi Uddin","doi":"10.1016/j.prmcm.2025.100734","DOIUrl":"10.1016/j.prmcm.2025.100734","url":null,"abstract":"<div><h3>Background</h3><div><em>Syzygium grande</em> (syn. Eugenia grandis), commonly known as the sea apple, is a medicinally important plant that has been traditionally used to treat various ailments, including coughs, hemorrhoids (piles), dental problems, dysentery, bronchitis, and diabetes. This study aimed to evaluate the <em>in vitro</em> anthelmintic, <em>in vivo</em> analgesic, and antidiarrheal activities of the methanolic extract of <em>S. grande</em> (MESG) using Swiss albino mice.</div></div><div><h3>Methods</h3><div>The anthelmintic activity was tested on the earthworm <em>Pheretima posthuma</em>. At the same time, analgesic effects were assessed using three models: the acetic acid-induced writhing test, the formalin-induced paw licking test, and the hot plate test. Antidiarrheal activity was evaluated using castor oil-induced diarrhea and the gastrointestinal motility test with charcoal meal. To support experimental findings, in silico methods such as molecular docking, ADME/T, PASS prediction, and network pharmacology were employed.</div></div><div><h3>Results</h3><div>Significant writhing inhibition was shown by MESG at 400 mg/kg—66.82% in the acetic acid test and 63.38% in the formalin test (p &lt; 0.001), and showed marked analgesic activity in the hot plate test. At 200 mg/kg, the extract also exhibited strong antidiarrheal effects (p &lt; 0.001). In the anthelmintic test, MESG at 10 μg/mL showed maximum efficacy comparable to levamisole (10 μg/mL). To clarify the multi-target potential of <em>S. grande</em> phytochemicals in modifying key proteins involved in pharmacological processes, this study combines network pharmacology with molecular docking. Molecular docking results revealed that gamma-sitosterol exhibited the highest binding affinity against 6COX, 5ZHP, and 1SA0 protein targets, suggesting strong potential as a drug candidate due to its favorable interactions.</div></div><div><h3>Conclusion</h3><div>Based on both experimental and computational evidence, MESG shows promise as a potential source of natural analgesic, antidiarrheal, and anthelmintic agents, warranting further research for drug development.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"18 ","pages":"Article 100734"},"PeriodicalIF":0.0,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145750172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The anti-tumor effect of chinese medicine extracts camptothecin -AS1411 system","authors":"Zhanbiao He ,&nbsp;Zhen Wang ,&nbsp;Lu Ga ,&nbsp;Jun Ai ,&nbsp;Xin Tong","doi":"10.1016/j.prmcm.2025.100732","DOIUrl":"10.1016/j.prmcm.2025.100732","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Introduction&lt;/h3&gt;&lt;div&gt;The AS1411 aptamer has shown great potential in the development of targeted anti-tumor drug delivery systems. &lt;strong&gt;Camptothecin (CPT, Xǐshù Jiǎn)&lt;/strong&gt;, a cytotoxic quinoline alkaloid first isolated from the traditional Chinese medicine &lt;em&gt;Camptotheca acuminata&lt;/em&gt; (Xǐshù), has been extensively used in various Chinese medicinal preparations including Xi Shu Injection and compound formulations combined with other herbs for cancer treatment. Despite its potent antitumor activity demonstrated in both traditional applications and modern clinical studies, the clinical application of CPT is limited by poor water solubility, low stability, and lack of tumor selectivity. Although some studies have combined AS1411 with chemotherapeutic drugs, the precise binding mechanism between the AS1411 G-quadruplex and small molecule drugs like CPT remains unclear. A systematic investigation into their interaction is crucial for the rational design of efficient and targeted drug delivery systems that can enhance the therapeutic potential of this important TCM-derived compound.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;In this work, we first synthesized a CPT-AS1411 complex and confirmed its formation. Subsequently, we employed a suite of biophysical techniques to decipher the binding mechanism between CPT and the AS1411 G-quadruplex. UV–Vis absorption and steady-state fluorescence spectroscopy were used to verify complex formation and determine the quenching constant. Ethidium bromide (EtBr) displacement assays were conducted to probe the binding mode (e.g., intercalation or groove binding). Circular dichroism (CD) spectroscopy was utilized to monitor conformational changes in the AS1411 structure upon CPT binding. Furthermore, the effects of ionic strength and the stability of the complex under different storage conditions were evaluated.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;UV–Vis and fluorescence spectroscopy confirmed the formation of the CPT-AS1411 complex. The calculated quenching constant (K∼SV∼ = 0.5135 M⁻¹, K∼q∼ = 5.135 × 10⁷ M⁻¹·S⁻¹) indicated a static quenching process, suggesting the formation of a ground-state complex. EtBr displacement studies and CD spectral analysis collectively demonstrated a non-intercalative binding mode between CPT and AS1411. Investigations into ionic strength revealed that electrostatic interactions played a minor role in complex formation. Stability studies indicated that the CPT-AS1411 complex was more stable when stored at -4 °C compared to room temperature.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;This study comprehensively elucidates the non-intercalative binding mechanism between CPT and the AS1411 G-quadruplex through multi-spectroscopic methods. Our findings provide crucial molecular-level insights and a solid experimental foundation for the future development of AS1411-based targeted delivery systems for camptothecin and its derivatives, potentially enhancing their therapeutic efficacy a","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"18 ","pages":"Article 100732"},"PeriodicalIF":0.0,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145694951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}