Pharmacological Research - Natural Products最新文献_第9页

From biodiversity to clinical translation: A global review of lichen-derived natural products and their pharmacological potential 从生物多样性到临床转化：地衣衍生的天然产物及其药理潜力的全球综述

Pharmacological Research - Natural Products

Pub Date : 2025-12-09 DOI: 10.1016/j.prenap.2025.100456

Meixia Yang , Lisha Lyu , Xinyu Wang , Shengying Lin , Ka Wing Leung , Tina Tingxia Dong , Lisong Wang , Karl Wah Keung Tsim

Lichens, which are unique ecosystems formed by fungi and photosynthetic partners like algae or cyanobacteria, hold great potential for drug discovery. This review compiled four decades of research to create the first comprehensive global database of 14,230 lichen species, among which 116 were highlighted for proven medicinal properties. Our spatial analysis identified key regions for therapeutic potential, such as Europe, coastal North America, East Asia, and Oceania, with the Parmeliaceae family was found to contain the most bioactive species. We systematically described 82 lichen-derived metabolites with confirmed pharmacological effects. Depsidones show promise as agents against oxidative stress and cancer; depsides/polysaccharides demonstrate efficacy in modulating neuroinflammation and immune response; and terpenoids contribute to addressing antimicrobial resistance. Cytotoxic, anticancer, anti-tumor, anti-microbial, and antioxidant activities are associated with the largest number of medicinal species. This research bridges traditional knowledge of lichens with modern pharmacology, identifying 195 medicinal species that need further validation for clinical use. We also discussed challenges in translating this knowledge into practice, such as ensuring sustainable biomass, optimizing bioavailability, and ensuring clinical safety, and we suggested lichen-specific guidelines for these processes. As lichen metabolites offer compelling opportunities to tackle pressing global health issues, such as antimicrobial resistance, neurodegenerative disorders, and cancer, we advocate for lichen metabolites as promising solutions for future pharmaceutical development.

地衣是由真菌和藻类或蓝藻等光合作用伙伴形成的独特生态系统，具有巨大的药物发现潜力。这篇综述汇编了40年的研究，建立了第一个全面的全球地衣数据库，包括14230种地衣，其中116种被证明具有药用价值。我们的空间分析确定了治疗潜力的关键区域，如欧洲、北美沿海、东亚和大洋洲，其中发现Parmeliaceae家族含有最具生物活性的物种。我们系统地描述了82种地衣衍生的代谢物，证实了其药理作用。作为抗氧化应激和癌症的药物，Depsidones大有希望；深度/多糖在调节神经炎症和免疫反应方面表现出功效；萜类化合物有助于解决抗菌素耐药性问题。细胞毒性、抗癌、抗肿瘤、抗微生物和抗氧化活性与数量最多的药用物种有关。这项研究将地衣的传统知识与现代药理学联系起来，确定了195种需要进一步验证以供临床使用的药用物种。我们还讨论了将这些知识转化为实践的挑战，例如确保可持续生物量，优化生物利用度和确保临床安全性，并针对这些过程提出了地衣特定指南。由于地衣代谢物为解决紧迫的全球健康问题（如抗菌素耐药性、神经退行性疾病和癌症）提供了令人信服的机会，我们提倡将地衣代谢物作为未来药物开发的有希望的解决方案。

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引用次数: 0

Shrimp-derived extracts as antibacterial, antioxidant, and burn wound-healing agents: Comprehensive in vitro and in vivo evaluation from Yemeni coastal resources 虾源提取物作为抗菌、抗氧化和烧伤创面愈合剂：也门沿海资源的体外和体内综合评价

Pharmacological Research - Natural Products

Pub Date : 2025-12-09 DOI: 10.1016/j.prenap.2025.100454

Wafa M. Al-Madhagi , Mohammed A. Alkhawlani , Majed Alwan , Saleh Alomaisi , Maged Al-Najar

This study was aimed at evaluating shrimp extract formulations derived from Penaeus indicus for their antibacterial, antioxidant, and burn wound healing potential. Shrimp specimens were collected from coastal regions in Yemen and processed to separate shells and bodies. Chitin was isolated from shells via deproteinization and demineralization and subsequently converted to chitosan through deacetylation, while glucosamine sulphate was extracted from both shells and bodies using optimized hydrolysis protocols. The extracts were characterized by Fourier-transform infrared spectroscopy, melting point analysis, and UV–visible spectrophotometry. Antimicrobial activity was assessed using broth microdilution and disc diffusion assays against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans. Antioxidant capacity was determined via the DPPH assay. In vivo burn wound healing efficacy was examined in murine models using both powder and ointment formulations of chitosan. Results revealed that chitosan exhibited significant antibacterial activity, with minimum inhibitory concentrations ranging from 0.0064 to 0.89 mg/mL, and the shell-derived glucosamine sulphate extract demonstrated potent free radical scavenging (IC₅₀ = 2.82 mg/μL). Moreover, 10 % chitosan formulations markedly accelerated wound healing, as evidenced by rapid re-epithelialization, reduced inflammation, and enhanced collagen deposition compared to lower concentrations and commercial treatments. Histopathological analysis confirmed superior tissue recovery in chitosan-treated groups, characterized by abundant collagen fibers, minimal inflammatory cells, and evidence of hair follicle regeneration. In conclusion, shrimp extract formulations, particularly those enriched with chitosan, offer a promising, cost-effective therapeutic strategy for burn wound management in resource-limited settings.

本研究旨在评价从印度对虾中提取的虾提取物的抗菌、抗氧化和烧伤愈合潜力。从也门沿海地区收集虾标本，并对其进行处理，以分离壳和体。通过脱蛋白和脱矿从壳中分离甲壳素，然后通过脱乙酰转化为壳聚糖，同时通过优化的水解方案从壳和体中提取硫酸氨基葡萄糖。采用傅里叶变换红外光谱、熔点分析和紫外可见分光光度法对提取物进行了表征。采用肉汤微稀释法和圆盘扩散法对大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌和白色念珠菌进行抑菌活性评估。通过DPPH法测定抗氧化能力。研究了壳聚糖粉剂和软膏制剂在小鼠模型中烧伤创面的体内愈合效果。结果表明，壳聚糖具有显著的抗菌活性，最低抑菌浓度范围为0.0064至0.89 mg/mL，壳源性硫酸氨基葡萄糖提取物具有有效的自由基清除能力（IC₅₀= 2.82 mg/μL）。此外，与低浓度和商业治疗相比，10% %壳聚糖配方显著加速了伤口愈合，证明了快速的再上皮化，减少炎症和增强胶原沉积。组织病理学分析证实，壳聚糖处理组的组织恢复良好，其特点是胶原纤维丰富，炎症细胞最少，毛囊再生。总之，虾提取物制剂，特别是那些富含壳聚糖的制剂，在资源有限的情况下为烧伤创面管理提供了一种有前途的、具有成本效益的治疗策略。

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引用次数: 0

Synthesis of chitosan nanoparticles from cuttlebone of Sepia pharaonis (Ehrenberg, 1831) and its antimicrobial effects against oral pathogens 法老乌贼骨制备壳聚糖纳米颗粒（Ehrenberg, 1831）及其对口腔病原体的抗菌作用

Pharmacological Research - Natural Products

Pub Date : 2025-12-01 DOI: 10.1016/j.prenap.2025.100438

Amurthaa Varshini Ragunath , Annathai Pitchai , Pasiyappazham Ramasamy

This study investigates the synthesis of chitosan nanoparticles (CSNPs) derived from the cuttlebone of Sepia pharaonis and evaluates their antimicrobial efficacy. Chitosan was extracted by sequential removal of minerals, proteins, and acetyl groups, followed by nanoparticle formation through ionic cross-linking with sodium tripolyphosphate (TPP). The resulting CSNPs were characterized using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). SEM imaging confirmed a predominantly spherical morphology with particle sizes ranging from 85 to 130 nm. XRD analysis revealed prominent peaks at 2θ = 20° and 25°, indicating crystallinity, while the absorption band at 1632 cm⁻¹ in FTIR spectra corresponded to N–H bending vibrations, confirming the interaction between chitosan and TPP. Antimicrobial activity, assessed via the agar well diffusion method, demonstrated inhibition zones of 24 ± 2.25 mm against Escherichia coli and 19 ± 1.53 mm against Streptococcus mutans. Additionally, E. coli exhibited greater sensitivity, with a minimum inhibitory concentration (MIC) of 1.0 mg/ml, whereas S. mutans required 1.5 mg/ml for comparable inhibition. These findings highlight the potential of CSNPs from S. pharaonis cuttlebone as effective antimicrobial agents for biomedical applications.

研究了以法老乌贼骨为原料制备壳聚糖纳米颗粒（csnp）的工艺，并对其抗菌效果进行了评价。壳聚糖的提取方法包括：依次去除矿物质、蛋白质和乙酰基，然后与三聚磷酸钠（TPP）离子交联形成纳米颗粒。利用傅里叶变换红外光谱（FTIR）、x射线衍射（XRD）和扫描电镜（SEM）对所得csnp进行了表征。扫描电镜成像证实了主要的球形形貌，粒径范围为85 ~ 130 nm。XRD分析发现，在2θ = 20°和25°处有明显的结晶峰，FTIR光谱中在1632 cm⁻¹ 处的吸收带对应于N-H弯曲振动，证实了壳聚糖与TPP之间的相互作用。通过琼脂孔扩散法评估抗菌活性，对大肠杆菌的抑制区为24±2.25 mm，对变形链球菌的抑制区为19±1.53 mm。此外，大肠杆菌表现出更高的敏感性，最低抑制浓度（MIC）为1.0 mg/ml，而变形链球菌需要1.5 mg/ml才能达到类似的抑制效果。这些发现突出了S. pharaonis海螵蛸csnp作为生物医学应用的有效抗菌剂的潜力。

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引用次数: 0

Deciphering the molecular mechanism of Hericium erinaceus secondary metabolites and their impact on gene regulatory networks in hepatic encephalopathy 破解猴头菌次生代谢产物的分子机制及其对肝性脑病基因调控网络的影响

Pharmacological Research - Natural Products

Pub Date : 2025-12-01 DOI: 10.1016/j.prenap.2025.100446

Rajappan Chandra Satish Kumar , Ramesh Venkatachalapathy , Sankari Alagukumar , Akash Jayaraman , Chittaranjan Das , Patana Teng-Umnuay

Hepatic encephalopathy (HE) is a severe neuropsychiatric disorder associated with liver dysfunction, leading to cognitive and motor impairments. Conventional treatments like lactulose and rifaximin often have limited efficacy and side effects, necessitating novel therapeutic strategies. Hericium erinaceus, an edible medicinal mushroom, exhibits hepatoprotective, neuroprotective, and gut-modulating properties. This study employs an integrated computational approach, including network pharmacology, molecular docking, Density Functional Theory (DFT), and Molecular Dynamics (MD) simulations, to evaluate the therapeutic potential of H. erinaceus secondary metabolites (erinacerin I, erinacerin L, and 1-D-arabinitol monolinoleate) against HE-related targets. Protein-protein interaction (PPI) analysis identified key targets, including GAPDH, AKT1, TNF, SRC, ALB, STAT3, and EGFR, involved in HE pathophysiology. Molecular docking revealed strong binding affinities of these metabolites to the identified targets, suggesting their potential to modulate HE-related pathways. DFT analysis indicated that erinacerin L is highly reactive, while 1-D-arabinitol monolinoleate exhibits greater stability. MD simulations confirmed the stability of protein-ligand complexes, supporting their pharmacological viability. These findings suggest that H. erinaceus metabolites are promising candidates for HE treatment, pending experimental validation in preclinical and clinical studies.

肝性脑病（HE）是一种与肝功能障碍相关的严重神经精神疾病，可导致认知和运动障碍。传统的治疗方法如乳果糖和利福昔明的疗效和副作用往往有限，需要新的治疗策略。猴头菌（Hericium erinaceus）是一种药用食用菌，具有保护肝脏、保护神经和调节肠道的特性。本研究采用综合计算方法，包括网络药理学、分子对接、密度泛函数理论（DFT）和分子动力学（MD）模拟，评估了H. erinaceus次级代谢物（erinacerin I、erinacerin L和1- d -阿拉伯糖醇单油酸酯）对he相关靶点的治疗潜力。蛋白-蛋白相互作用（PPI）分析确定了参与HE病理生理的关键靶点，包括GAPDH、AKT1、TNF、SRC、ALB、STAT3和EGFR。分子对接显示，这些代谢物与鉴定的靶标具有很强的结合亲和力，表明它们可能调节he相关途径。DFT分析表明，erinacerin L具有较高的活性，而1- d -阿拉伯糖醇单油酸酯具有较高的稳定性。MD模拟证实了蛋白质配体复合物的稳定性，支持其药理活性。这些发现表明，羊角弧菌代谢物是治疗HE的有希望的候选者，有待于临床前和临床研究的实验验证。

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引用次数: 0

Antidiabetic potential of Ficus septica targeting aldose reductase and glycogen synthase kinase 3β 针对醛糖还原酶和糖原合成酶激酶3β的无花果的降糖潜力

Pharmacological Research - Natural Products

Pub Date : 2025-12-01 DOI: 10.1016/j.prenap.2025.100440

Dintakurthi Sree Naga Bala Krishna Prasanth , Praveen Kumar Pasala , Deepak A. Yaraguppi , Johra Khan , Mithun Rudrapal

The search for effective therapeutic targets to combat diabetes has centered around two key players, human aldose reductase (ALR) and glycogen synthase kinase 3β (GSK 3β). Recent studies have generated interest in the potential of plant compounds to manage diabetes, prompting an investigation into the compounds of Ficus septica. This study sought to reveal the untapped potential of these compounds through comprehensive analyses, including docking studies, to identify dual inhibitors of ALR and GSK 3β. The results unveiled four promising compounds including genistein, aesculin, pungenin, and norruspolin. Genistein which demonstrated remarkable binding energy and molecular dynamic studies revealed its significant potential. Among the leads, genistein was found to be the most prominent compound with the highest binding energy of −7.4 and −8.4 kcal/mol with ALR and GSK 3β, respectively. Further, genistein was subjected to molecular dynamic studies, which indicated a binding affinity of −69.584 ± 8.535 and −62.137 ± 12.929 kJ/mol with GSK 3β and ALR, respectively, over a duration of 100 ns. Our study revealed that genistein demonstrated good binding affinity to ALR and GSK3β, as determined through molecular docking, dynamic simulation. This study identified four compounds from Ficus septica as dual inhibitors of ALR and GSK 3β using molecular docking studies; and they are genistein, aesculin, pungenin, and norruspolin.

针对糖尿病的有效治疗靶点的研究主要集中在两个关键因素上，即人醛糖还原酶（ALR）和糖原合成酶激酶3β (GSK 3β)。最近的研究对植物化合物治疗糖尿病的潜力产生了兴趣，促使对榕树化合物的调查。本研究试图通过包括对接研究在内的综合分析来揭示这些化合物尚未开发的潜力，以确定ALR和GSK 3β的双重抑制剂。结果揭示了四种很有前景的化合物，包括染料木素、七叶草素、刺黄素和诺里斯波林。染料木黄酮的结合能和分子动力学研究显示了其巨大的潜力。其中染料木素与ALR和GSK 3β的结合能最高，分别为−7.4和−8.4 kcal/mol。此外，染料木黄酮进行了分子动力学研究，结果表明其与GSK 3β和ALR的结合亲和力分别为- 69.584 ± 8.535和- 62.137 ± 12.929 kJ/mol，持续时间为100 ns。本研究通过分子对接、动态模拟发现染料木素对ALR和GSK3β具有良好的结合亲和力。本研究通过分子对接研究，从榕树中鉴定出4个具有ALR和GSK 3β双重抑制剂的化合物；它们是染料木黄酮，七叶黄素，刺黄素，和norruspolin。

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引用次数: 0

Virtual screening of flavonoids as potential inhibitors of Candida species: An in silico approach utilizing ADMET and molecular docking studies 虚拟筛选类黄酮作为潜在的假丝酵母菌抑制剂：利用ADMET和分子对接研究的计算机方法

Pharmacological Research - Natural Products

Pub Date : 2025-12-01 DOI: 10.1016/j.prenap.2025.100442

Subiya Showkat , Thiruppathi Senthil Kumar

Fungal infections account for approximately 1.7 million deaths annually, predominantly affecting immunocompromised individuals. The incidence of Candida infections has risen since the 1970s due to increased opportunistic infections, improved detection of hospital-acquired fungal infections, and antifungal resistance from prolonged treatments. This study performed virtual screening of four flavonoids: apigenin, catechin, kaempferol, and quercetin using molecular docking, in silico physicochemical analyses, drug-likeness evaluation, and ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) prediction to identify potential therapeutic alternatives for Candidiasis. These compounds were tested against four Candida albicans target enzymes: N-myristoyl transferase (PDB ID: 1IYL), Lanosterol 14-alpha Demethylase (PDB ID: 5V5Z), Dihydrofolate Reductase (PDB ID: 1M78), and Secreted Aspartic Protease (PDB ID: 1ZAP). The flavonoids exhibited significant inhibitory effects compared to fluconazole and voriconazole. Protein-ligand interaction analysis confirmed effective binding to crucial amino acid residues. Furthermore, all compounds adhered to Lipinski’s Rule of Five and demonstrated favorable drug-likeness and ADMET profiles, supporting their potential as therapeutic leads. These findings highlight flavonoids as promising candidates for further optimization in the treatment of Candidiasis.

真菌感染每年造成约170万人死亡，主要影响免疫功能低下的个体。念珠菌感染的发病率自20世纪70年代以来一直上升，原因是机会性感染增加，医院获得性真菌感染的检测改善，以及长期治疗产生的抗真菌耐药性。本研究通过分子对接、计算机理化分析、药物相似性评价和ADMET（吸收、分布、代谢、排泄和毒性）预测，对芹菜素、儿茶素、山奈酚和槲皮素四种黄酮类化合物进行虚拟筛选，以确定假丝酵母菌病的潜在治疗方案。这些化合物对4种白色念珠菌靶酶：n -肉豆油酰基转移酶（PDB ID: 1IYL）、羊毛甾醇14- α去甲基化酶（PDB ID: 5V5Z）、二氢叶酸还原酶（PDB ID: 1M78）和分泌天冬氨酸蛋白酶（PDB ID: 1ZAP）进行了抑菌试验。与氟康唑和伏立康唑相比，黄酮类化合物具有明显的抑制作用。蛋白质-配体相互作用分析证实了与关键氨基酸残基的有效结合。此外，所有化合物都遵循Lipinski的五法则，并表现出良好的药物相似性和ADMET谱，支持它们作为治疗先导的潜力。这些发现突出了类黄酮作为进一步优化治疗念珠菌病的有希望的候选者。

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引用次数: 0

Natural guardians as radioprotective complements in oncological therapy: A review 天然保护剂在肿瘤治疗中的放射防护作用综述

Pharmacological Research - Natural Products

Pub Date : 2025-12-01 DOI: 10.1016/j.prenap.2025.100441

Wafa Zennouhi , Boutaina Addoum , Mohamed Bourhia , Farid Khallouki , Mohammed Attaleb , Mohamed El Mzibri , Imane Chaoui , Imen Ghzaiel , Fatiha Brahmi , Atanas G. Atanasov , Anne Vejux , Gérard Lizard , Laila Benbacer

Ethnopharmacological relevance

Modern research has increasingly focused on the traditional use of plants for radioprotection. Plant extracts, rich in bioactive compounds, represent promising candidates for mitigating radiation-induced damage. This ethnopharmacological perspective highlights the value of ancestral knowledge in developing complementary therapeutic strategies.

Purpose

Radiotherapy remains a cornerstone of cancer treatment, yet it is often accompanied by adverse effects that impair patients' quality of life. The search for effective, safe, and natural radioprotective agents is therefore of high importance.

Methods

Following PRISMA guidelines, a systematic search was conducted using PubMed and Google Scholar for articles published between April 2019 and February 2025. Keywords included “radioprotective potential” AND (“plant extracts” OR “natural radioprotectors” OR “radioprotection”). Only studies reporting experimental or clinical evidence on the radioprotective effects of plant-derived compounds were selected.

Results

Numerous studies demonstrate that plant-based compounds may protect against ionizing radiation through mechanisms such as reactive oxygen species scavenging, DNA repair, anti-inflammatory activity, and regulation of apoptosis-related pathways. These effects are primarily attributed to secondary metabolites, including polyphenols, vitamins, terpenoids, alkaloids, and glucosinolates.

Conclusion

This review compiles recent advances on the use of plant-based natural products for radioprotection. These findings encourage further research into phytomedicine as a supportive approach to reduce radiation-induced side effects and improve cancer care.

现代研究越来越关注植物的辐射防护传统用途。富含生物活性化合物的植物提取物是减轻辐射引起的损害的有希望的候选者。这种民族药理学观点强调了祖先知识在开发补充治疗策略中的价值。目的放疗仍然是癌症治疗的基石，但它往往伴随着影响患者生活质量的副作用。因此，寻找有效、安全和天然的辐射防护剂是非常重要的。方法根据PRISMA指南，使用PubMed和谷歌Scholar对2019年4月至2025年2月间发表的文章进行系统检索。关键词包括“辐射防护潜力”和（“植物提取物”或“天然辐射防护剂”或“辐射防护”）。只有报告了实验或临床证据的植物源化合物的辐射防护作用的研究被选择。结果大量研究表明，植物基化合物可能通过活性氧清除、DNA修复、抗炎活性和调节细胞凋亡相关途径等机制来保护细胞免受电离辐射的伤害。这些作用主要归因于次生代谢物，包括多酚、维生素、萜类、生物碱和硫代葡萄糖苷。结论本文综述了植物性天然产物在放射性防护方面的最新进展。这些发现鼓励进一步研究植物医学作为一种支持方法，以减少辐射引起的副作用和改善癌症治疗。

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引用次数: 0

Hair growth promoting effects of Kesh-Kanti-Hair-Expert-Oil are mediated through keratinocyte proliferation and modulation in follicular microenvironment Kesh-Kanti-Hair-Expert-Oil促进头发生长的作用是通过角化细胞增殖和调节毛囊微环境介导的

Pharmacological Research - Natural Products

Pub Date : 2025-12-01 DOI: 10.1016/j.prenap.2025.100437

Acharya Balkrishna , Pratibha Singh , Sonam Sharma , Yash Varshney , Sudeep Verma , Sandeep Sinha , Rishabh Dev , Anurag Varshney

The premature shift from anagen phase to telogen phase during hair growth cycle is the hallmark of progressive alopecia which is characterized by miniaturization of hair follicles and failure to regrow hair effectively. Existing treatment options demonstrate limited efficacy due to the unpredictable nature of this disease. Present study examined the hair growth-promoting potential of Kesh-Kanti-Advance-Herbal-Hair-Expert-Oil (coded as, KKEO). It is a polyherbal formulation that contains various herbs with known hair regrowth properties. The phytometabolite analysis conducted using GC/MS-MS and UPLC/MS-QToF identified several compounds known for their anti-oxidant, anti-inflammatory, and hair growth-promoting properties. These phytometabolites included camphor, eucalyptol, linalool, β-himachalene, coumarin, atalantoflavone, linoleic acid and oleic acid. In vitro studies on human keratinocytes (HaCaT) and human hair dermal papilla cells (HHDPCs) demonstrated that KKEO enhanced proliferation of keratinocytes and mitigated loss of cell viability induced by UVB, SLS, and CHP. Additionally, KKEO upregulated the expression of genes related to hair growth cycle including β-Catenin, IGF, KGF and VEGF in HaCaT cells. Remarkably, conditioned medium (KKEO-CM) from KKEO-treated HaCaT cells stimulated proliferation of HHDPCs and augmented the expression of genes associated with hair growth cycle, particularly β-Catenin and SOX2. KKEO-CM also abrogated 5α-Dihydrotestosterone (DHT)-induced apoptosis in HHDPCs. Interestingly, topical application of KKEO to shaved dorsal skin of Wistar rats demonstrated significant enhancement in hair growth parameters. USFDA approved drug Minoxidil was used as a positive control. Overall, present study underscores the effectiveness of KKEO in stimulating hair growth via regulation of follicular microenvironment, promoting keratinocyte proliferation and activating dermal papilla cells.

在头发生长周期中，从生长期到休止期的过早转变是进行性脱发的标志，其特征是毛囊小型化和不能有效地再生头发。由于这种疾病的不可预测性，现有的治疗方案显示出有限的疗效。本研究考察了kech - kanti - advance - herb - hair - expert - oil（编码为，KKEO）的促发潜力。这是一个多草药配方，包含各种草药与已知的头发再生特性。利用GC/MS-MS和UPLC/MS-QToF进行的植物代谢物分析鉴定出几种已知具有抗氧化、抗炎和促进头发生长特性的化合物。这些植物代谢物包括樟脑、桉树醇、芳樟醇、β-马马烯、香豆素、去乙酰黄酮、亚油酸和油酸。对人角质形成细胞（HaCaT）和人毛真皮乳头细胞（HHDPCs）的体外研究表明，KKEO增强了角质形成细胞的增殖，减轻了UVB、SLS和CHP诱导的细胞活力丧失。此外，KKEO上调HaCaT细胞中与毛发生长周期相关的基因β-Catenin、IGF、KGF和VEGF的表达。值得注意的是，kkeo处理的HaCaT细胞的条件培养基（KKEO-CM）刺激了HHDPCs的增殖，并增加了与头发生长周期相关的基因的表达，特别是β-Catenin和SOX2。KKEO-CM还能消除5α-二氢睾酮（DHT）诱导的HHDPCs细胞凋亡。有趣的是，将KKEO局部应用于Wistar大鼠剃光的背部皮肤，可以显著增强毛发生长参数。采用美国食品药品监督管理局批准的药物米诺地尔作为阳性对照。总的来说，本研究强调了KKEO通过调节毛囊微环境、促进角质细胞增殖和激活真皮乳头细胞来刺激毛发生长的有效性。

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引用次数: 0

Rhizophora mucronata flower extracts as a source of anticancer and antimicrobial agents: GC-MS, FTIR and molecular docking approaches 根参花提取物作为抗癌和抗菌药物的来源：GC-MS、FTIR和分子对接方法

Pharmacological Research - Natural Products

Pub Date : 2025-12-01 DOI: 10.1016/j.prenap.2025.100445

Munusamy Muthukumaran , Fredrick Raja Edwardpaul , Noorjahan Abdulbari , Giridharan Bupesh , Dharmalingam Kirubakaran

Rhizophora mucronata, a mangrove species traditionally used in medicine, was investigated for the anticancer and antimicrobial potential of its flowers, with a focus on phytochemical composition. Fresh flowers were extracted using water, ethanol, and ethyl acetate, and the extracts were evaluated for antimicrobial activity, anticancer effects, and phytochemical content. Antimicrobial efficacy was tested against bacterial and fungal strains, while anticancer activity was assessed on A549 (lung) and MDA-MB-231 (breast) cancer cell lines. Phytochemical profiling using FTIR revealed functional groups such as phenols, alcohols, and amides, while GC-MS of the ethyl acetate extract identified key bioactive metabolites including 4-(3-hydroxybutyl) phenol, 2-methyl-5-(1,2,2-trimethylcyclopentyl) phenol, and 9,12-octadecadienoic acid. The ethyl acetate extract exhibited the highest flavonoid content and showed strong antimicrobial activity, comparable to gentamicin, with MIC values of 150 µg/mL for bacteria and 50 µg/mL for fungi. It also demonstrated significant anticancer activity against both tested cancer cell lines. Molecular docking revealed strong interactions of the identified compounds with peptidyl-proline isomerases, supporting their therapeutic relevance. Overall, this study highlights the pharmaceutical potential of R. mucronata flower extracts, particularly the ethyl acetate fraction, as promising sources of antimicrobial and anticancer agents.

摘要研究了一种传统药用红树植物——毛根茎（Rhizophora mucronata）花的抗癌和抗菌潜力，重点研究了其植物化学成分。用水、乙醇和乙酸乙酯提取鲜花，并对提取物的抑菌活性、抗癌作用和植物化学成分进行了评价。对细菌和真菌菌株进行抑菌效果测试，对A549（肺）和MDA-MB-231（乳腺癌）癌细胞进行抑菌活性评估。利用FTIR进行植物化学分析，发现了酚类、醇类和酰胺类等官能团，而乙酸乙酯提取物的GC-MS鉴定了关键的生物活性代谢物，包括4-（3-羟基丁基）苯酚、2-甲基-5-（1,2,2-三甲基环戊基）苯酚和9,12-十八烯二烯酸。乙酸乙酯提取物类黄酮含量最高，抗菌活性与庆大霉素相当，对细菌的MIC值为150 µg/mL，对真菌的MIC值为50 µg/mL。它还对两种测试的癌细胞系显示出显著的抗癌活性。分子对接揭示了鉴定的化合物与肽酰脯氨酸异构酶的强相互作用，支持其治疗相关性。总的来说，本研究强调了麻花提取物的药用潜力，特别是乙酸乙酯部分，作为抗微生物和抗癌药物的有希望的来源。

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引用次数: 0

Exploring medicinal plant diversity in the Serra da Estrela Natural Park (Portugal): Assessing the influence of socioeconomic variables on medicinal plant knowledge (Part II) 探索Serra da Estrela自然公园（葡萄牙）的药用植物多样性：评估社会经济变量对药用植物知识的影响（第二部分）

Pharmacological Research - Natural Products

Pub Date : 2025-12-01 DOI: 10.1016/j.prenap.2025.100439

Radhia Aitfella Lahlou , Filomena Carvalho , Maria João Pereira , João Lopes , Luís R. Silva

Background

Medicinal plant knowledge underpins community health, yet its distribution within populations is uneven. Mountain protected areas in Europe remain understudied in this regard.

Objective

To quantify intracultural variation in medicinal-plant knowledge in the Serra da Estrela Natural Park and assess associations with socio-demographic and geographic factors.

Methods

Cross-sectional survey of 286 informants across five municipalities documenting 133 medicinal species. We analysed patterns using non-metric multidimensional scaling (NMDS), permutational multivariate analysis of variance (PERMANOVA), and generalised linear models (GLMs). A network approach summarised links between frequently cited plant families and disease categories.

Result

Knowledge composition and richness varied significantly among social groups. Women contributed 3507 use reports, compared to 1392 by men. PERMANOVA showed non-random differences by marital status (F = 36.55, p = 0.0001; Bonferroni pairwise p = 0.0006). GLMs identified geographic and social predictors: for digestive uses, Celorico da Beira (B = −0.302, 95 % CI −0.512 to −0.091, p = 0.005) and Guarda (B = −0.540, 95 % CI −0.755 to −0.325, p < 0.001) listed fewer species than Gouveia; for respiratory uses, several municipalities listed more species than Gouveia (e.g., Seia B = 0.769, p < 0.001). Singles reported fewer genitourinary symptoms than widows (B = −0.478, p = 0.039).

Conclusions

Medicinal-plant knowledge in Serra da Estrela is intraculturally structured by municipality and social factors; reporting effect sizes and p-values provides decision-relevant evidence for conservation and rural-health planning. Quantifying this variability provides actionable evidence for safeguarding biocultural heritage and for integrating local knowledge into conservation and rural-health planning in rapidly changing mountain landscapes.

药用植物知识是社区卫生的基础，但其在人群中的分布并不均衡。在这方面，对欧洲山区保护区的研究仍然不足。目的量化Serra da Estrela自然公园药用植物知识的文化内变异，并评估其与社会人口和地理因素的关系。方法对来自5个直辖市的286名被调查者进行横断面调查，记录了133种药用植物。我们使用非度量多维尺度（NMDS）、置换多元方差分析（PERMANOVA）和广义线性模型（GLMs）分析模式。一个网络方法总结了经常被引用的植物科和疾病类别之间的联系。结果不同社会群体的知识构成和知识丰富程度存在显著差异。女性贡献了3507份使用报告，而男性贡献了1392份。PERMANOVA显示婚姻状况的非随机差异（F = 36.55, p = 0.0001；Bonferroni成对p = 0.0006）。GLMs确定了地理和社会预测因子：对于消化功能，Celorico da Beira （B = - 0.302, 95 % CI - 0.512至- 0.091,p = 0.005）和Guarda （B = - 0.540, 95 % CI - 0.755至- 0.325,p <； 0.001）列出的物种比Gouveia少；在呼吸方面，一些城市列出了比古维雅更多的种类（例如，Seia B = 0.769, p <； 0.001）。单身人士报告的泌尿生殖系统症状少于寡妇（B = - 0.478, p = 0.039）。结论埃斯特雷拉山区药用植物知识是由城市和社会因素构成的；报告效应大小和p值为保护和农村卫生规划提供决策相关证据。量化这种可变性为保护生物文化遗产和将当地知识纳入快速变化的山区景观的保护和农村卫生规划提供了可操作的证据。

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引用次数: 0