Plant Nano Biology最新文献_第3页

Utilizing Arthrospira platensis for the fabrication of zinc oxide nanoparticles: Analysis and assessment for enhancing drought tolerance in Sub1A QTL bearing rice seedlings 利用节肢动物制造纳米氧化锌颗粒：分析和评估提高Sub1A QTL水稻秧苗的抗旱能力

Plant Nano Biology

Pub Date : 2024-10-19 DOI: 10.1016/j.plana.2024.100101

Abir Das , Sayanti Bagchi , Sayan Pal , Anway Ganguly , Sudipta Kumar Sil , Malay Kumar Adak

In the present study, the underlying pathways for zinc oxide nanoparticles (ZnO-NPs) mediated modulation of oxidative stress under drought were evaluated in rice seedlings. Principally, rice cultivar (cv. Swarna Sub1) was used to assess the potential of the Sub1A QTL for its drought sensitivity in response to bio-fabricated ZnO-NPs. ZnO-NPs were synthesized from algal (Arthrospira platensis) extract and characterized for their opto-physical properties, confirming size (54 nm), hydrodynamicity (-18.54), amorphous-cubic shape and others features. Fifteen-day-old rice seedlings were primed with 25 ppm ZnO-NPs and exposed to 12 % polyethylene glycol (PEG) mediated drought stress (DS) for 7-days under laboratory conditions. Primarily, Sub1A QTL responded to drought-induced anoxic stress with a significant increase in the activities of alcohol dehydrogenase (447.41 %) and pyruvate decarboxylase (96.51 %) through ZnO-NPs sensitization. Plants recorded a significant reduction in root growth, which regained 89.25 % with ZnO-NPs treatments. ZnO-NPs also recovered relative water content (49 %), proline (99.2 %), and improved chlorophyll fluorescence (90.9 %) under stress. Furthermore, drought-induced membrane leakage was stabilized by reducing ionic conductivity through the distribution of wall-bound polyamines. A characteristic feature of fluorescence also reinforced the sustenance of photosynthetic activities by ZnO-NPs under drought. Alternatively, rice seedlings showed regulation of oxidative stress, where lipid peroxidation and protein carbonylation were reduced by 270 % and 178.2 %, respectively. This was observed with the minimization of superoxide and hydrogen peroxide concentrations by regulating apoplastic oxidase activity (117.64 %) with distinct polymorphisms in proteins. These observations suggest that ZnO-NPs can ameliorate drought-induced oxidative stress in rice, providing insights for improved nano-fertigation.

本研究评估了氧化锌纳米颗粒（ZnO-NPs）介导的水稻幼苗在干旱条件下调节氧化应激的基本途径。主要利用水稻栽培品种（cv. Swarna Sub1）来评估 Sub1A QTL 对生物制造的 ZnO-NPs 的干旱敏感性的潜力。ZnO-NPs 由藻类（Arthrospira platensis）提取物合成，并对其光物理性质、确认尺寸（54 nm）、水动力（-18.54）、无定形立方体形状及其他特征进行了表征。在实验室条件下，用 25 ppm ZnO-NPs 对 15 天大的水稻秧苗进行引诱，并将其置于 12 % 聚乙二醇（PEG）介导的干旱胁迫（DS）中 7 天。通过 ZnO-NPs 的敏化作用，Sub1A QTL 主要对干旱诱导的缺氧胁迫做出反应，醇脱氢酶（447.41 %）和丙酮酸脱羧酶（96.51 %）的活性显著增加。植物的根系生长明显减少，但在 ZnO-NPs 处理后恢复了 89.25%。ZnO-NPs 还能恢复胁迫下的相对含水量（49%）、脯氨酸（99.2%），并改善叶绿素荧光（90.9%）。此外，通过壁结合多胺的分布降低离子传导性，稳定了干旱引起的膜渗漏。荧光的特征也加强了 ZnO-NPs 在干旱条件下对光合作用的维持。此外，水稻秧苗还表现出对氧化应激的调节作用，其中脂质过氧化和蛋白质羰基化分别降低了 270% 和 178.2%。通过调节凋亡体氧化酶活性（117.64%），最大限度地降低了超氧化物和过氧化氢的浓度，同时蛋白质也出现了明显的多态性。这些观察结果表明，ZnO-NPs 可以改善干旱引起的水稻氧化应激，为改进纳米灌溉提供了启示。

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引用次数: 0

Nanomaterials as tools in plant transformation: A protoplast-centric perspective 作为植物转化工具的纳米材料：以原生质体为中心的视角

Plant Nano Biology

Pub Date : 2024-10-18 DOI: 10.1016/j.plana.2024.100100

Zhila Osmani , Lipu Wang , Wei Xiao , Marianna Kulka

Genetic engineering of plants can boost disease resistance, enhance crop traits, and ultimately improve agricultural productivity. Several approaches to plant bioengineering have been successful in recent decades. Nanomaterials (NMs) can be customized and fabricated with targeting capabilities, making them well-suited for bioengineering applications. These NMs include organic, inorganic, and composite materials with many different structures, including nanofibers, nanoparticles (NPs), and nanomembranes. Protoplasts are often used as target cells because they lack a cell wall and are more likely to endocytose NM. In this review, the efficacy of NMs in delivering genetic material to protoplasts is examined. The challenges associated with protoplast generation and optimization of protocols for transformation are explored and the possible advantages of NMs in this process are identified. The chemical properties of these NMs in relation to their potency is briefly discussed. Ultimately, this technology is evolving and our understanding of NMs and the requirement for migration through the cellular membrane is still missing several key pieces of information. The next decades will likely produce important new insights that will have important impacts in this field.

植物基因工程可以增强植物的抗病性，提高作物的性状，并最终提高农业生产率。近几十年来，有几种植物生物工程方法取得了成功。纳米材料（NMs）可以定制和制造，具有靶向能力，非常适合生物工程应用。这些纳米材料包括具有多种不同结构的有机、无机和复合材料，其中包括纳米纤维、纳米颗粒（NPs）和纳米膜。原生质体通常被用作靶细胞，因为它们缺乏细胞壁，更有可能内吞 NM。在这篇综述中，我们将探讨 NM 在向原生质体输送遗传物质方面的功效。探讨了与原生质体生成和转化方案优化相关的挑战，并指出了 NMs 在这一过程中可能具有的优势。还简要讨论了这些 NMs 与其效力相关的化学特性。归根结底，这项技术还在不断发展，而我们对 NMs 和通过细胞膜迁移的要求的理解还缺少一些关键信息。未来几十年可能会产生新的重要见解，对这一领域产生重要影响。

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引用次数: 0

Comparing the individual and combined effects of nano zinc and conventional zinc fertilization on growth, yield, phytochemical properties, antioxidant activity, and secoisolariciresinol diglucoside content in linseed 比较纳米锌和传统锌肥对亚麻籽的生长、产量、植物化学特性、抗氧化活性和仲异落叶松脂二葡萄糖苷含量的单独和综合影响

Plant Nano Biology

Pub Date : 2024-10-17 DOI: 10.1016/j.plana.2024.100098

Ragini Singh , Peer Saffeullah , Shahid Umar , Sageer Abass , Sayeed Ahmad , Noushina Iqbal

Zinc sulfate (ZnSO₄), a conventional Zn fertilizer, is widely used due to its high solubility and ease of application. In contrast, nano Zn represents an innovative approach, utilizing nanoscale particles to enhance Zn bioavailability and uptake efficiency. This study compares these two Zn fertilizers regarding their impact on plant growth, yield, Zn uptake, and overall crop quality. In our study, we explored the potential of nano Zn and ZnSO₄ by applying two different doses of each (100, 1000 ppm ZnO NP and 30, 50 kg ha⁻¹ ZnSO₄) both individually and in combination, to linseed accession. The results obtained showed the potential of nano Zn over conventional Zn fertilizer in terms of enhanced linseed growth and yield together with greater antioxidants enzyme, oil content, protein content, Zn accumulation, secoisolariciresinol diglucoside (SDG) content, and the accumulation of bioactive metabolites. Nanoscale ZnO (with particle size less than 100 nm) at a 1000 ppm concentration sped up growth, yield, increased SDG content, and antioxidant activity. However, when nano Zn (1000 ppm) was applied in combination with ZnSO₄ (30 kg ha⁻¹), it maximally enhanced plant fresh and dry weight, photosynthesis, and yield compared to their individual treatment. The combined application increased seed yield by 4.55 folds compared to the control. The treated plants were assessed for SDG content using liquid chromatography-mass spectrometry analysis (LC-MS), which showed maximum increase with 1000 ppm ZnO NP. SDG is a type of lignan known for their antioxidant properties and potential health benefits paving way for its pharmaceutical importance.

硫酸锌（ZnSO4）是一种传统的锌肥，因其溶解度高、易于施用而被广泛使用。相比之下，纳米锌代表了一种创新方法，它利用纳米级颗粒来提高锌的生物利用率和吸收效率。本研究比较了这两种锌肥对植物生长、产量、锌吸收和作物整体质量的影响。在我们的研究中，我们对亚麻籽品种施用了两种不同剂量的纳米锌和硫酸锌（分别为 100 和 1000 ppm ZnO NP 以及 30 和 50 kg ha-1 ZnSO4），既有单独施用，也有混合施用。研究结果表明，与传统锌肥相比，纳米锌具有提高亚麻籽生长和产量的潜力，同时还能提高抗氧化酶、油含量、蛋白质含量、锌积累、仲异落叶松脂素二葡萄糖苷（SDG）含量以及生物活性代谢物的积累。浓度为 1000 ppm 的纳米级氧化锌（粒径小于 100 nm）可加速生长、提高产量、增加 SDG 含量和抗氧化活性。然而，当纳米锌（1000 ppm）与硫酸锌（30 kg ha-1）联合施用时，与单独施用相比，能最大程度地提高植物的鲜重和干重、光合作用和产量。与对照组相比，联合施用可使种子产量提高 4.55 倍。使用液相色谱-质谱分析法（LC-MS）对处理过的植物进行了 SDG 含量评估，结果表明 1000 ppm ZnO NP 能最大程度地提高 SDG 含量。SDG 是一种木质素，因其抗氧化特性和潜在的健康益处而闻名，为其在医药方面的重要性铺平了道路。

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引用次数: 0

Opinion: Smart nanofertilizers for growth enhancement and stress resilience in agriculture 意见：智能纳米肥料促进农业生长和抗逆性

Plant Nano Biology

Pub Date : 2024-10-17 DOI: 10.1016/j.plana.2024.100095

Ritu Gill , Faheem Ahmed , Gopal Kalwan , Narendra Tuteja , Sarvajeet Singh Gill

Smart nanofertilizers (NFs) represent a promising frontier in agricultural technology, offering precise nutrient delivery and stress resilience to enhance crop growth and productivity while minimizing the environmental impact. This opinion article explores the potential of smart NFs in revolutionizing traditional fertilization practices and addressing the challenges facing modern agriculture. By harnessing the unique properties of nanomaterials, smart NFs including noble metal nanoparticles, green NFs, and novel NFs from industrial waste enable targeted nutrient delivery, enhanced nutrient use efficiency, and stress tolerance in plants. However, their widespread adoption faces regulatory, safety, and scalability challenges that require interdisciplinary collaboration and concerted efforts from stakeholders. Despite these hurdles, smart NFs hold immense promise for promoting sustainable agriculture and ensuring food security in frequently changing global climatic conditions.

智能纳米肥料（NFs）是农业技术中前景广阔的前沿领域，可提供精确的养分输送和抗逆性，从而提高作物的生长和产量，同时最大限度地减少对环境的影响。这篇观点文章探讨了智能 NF 在革新传统施肥方法和应对现代农业面临的挑战方面的潜力。通过利用纳米材料的独特性能，智能 NFs（包括贵金属纳米颗粒、绿色 NFs 和来自工业废料的新型 NFs）可实现有针对性的养分输送、提高养分利用效率和植物的抗逆性。然而，它们的广泛应用面临着监管、安全和可扩展性方面的挑战，需要跨学科合作和利益相关者的共同努力。尽管存在这些障碍，智能 NFs 仍为促进可持续农业和确保全球气候条件频繁变化时的粮食安全带来了巨大希望。

引用次数: 0

Interactive effect of biogenic nanoparticles and UV-B exposure on physio-biochemical behavior and secondary metabolism of Artemisia annua L 生物纳米粒子和紫外线-B 暴露对黄花蒿生理生化行为和次生代谢的交互影响

Plant Nano Biology

Pub Date : 2024-10-15 DOI: 10.1016/j.plana.2024.100097

Deepika Tripathi , Apoorva , Niraj Kumar Goswami , Shashi Pandey-Rai

Biosynthesized silver nanoparticles (AgNPs) are key nanomaterials with unique physio-chemical characteristics and diverse applications. Their strong absorption potential and antibacterial activity make them useful for agriculture, medicine and other industries. AgNPs boost plant growth and metabolism, especially under stress. However, the combined effects of AgNPs and UV-B exposure on plants are unknown. To elucidate the interactive effects of biosynthesized silver nanoparticles (AgNPs) and exposure of ultraviolet B (UV-B) on plant growth and metabolic processes, this study assessed the response of Artemisia annua under controlled in vitro conditions. In total, eight sets of plants were used with the alone/combined treatment of AgNPs and UV-B. For this purpose, spherical and averaged ∼ 31.8 nm in size AaAgNPs were synthesized. The photosynthetic pigments were calculated maximum with the alone treatment of 0.5 mg L⁻¹ AaAgNPs and combined treatment of 0.5 mg L⁻¹ AaAgNPs with 3 h UV-B, respectively. The results evidenced that the co-exposure of AaAgNPs and UV-B led to a significant balance in ROS production of A. annua; as well as improved antioxidative enzyme activity. Fluorescence and scanning electron microscopic (SEM) analysis indicated the enhancement of glandular trichomes (GT) area and density with the combined treatment of AaAgNPs and 3 h UV-B. In accordance with correlation between microscopic GT results, high concentration of artemisinin and up-regulation of related transcripts were found in A. annua plants treated with low concentrations of AaAgNPs and UV-B. Thus, it may be inferred that two distinct plant growth modulators, namely low-concentration biosynthesized AgNPs and short-term UV-B exposure, can enhance the physio-biochemical characteristics and production of secondary metabolites (specially artemisinin) in A. annua synergistically.

生物合成银纳米粒子（AgNPs）是一种重要的纳米材料，具有独特的物理化学特性和多种用途。其强大的吸收潜力和抗菌活性使其在农业、医药和其他行业中大有用武之地。AgNPs 可促进植物生长和新陈代谢，尤其是在胁迫条件下。然而，AgNPs 和紫外线照射对植物的综合影响尚不清楚。为了阐明生物合成的银纳米粒子（AgNPs）和紫外线 B（UV-B）照射对植物生长和代谢过程的交互作用，本研究在受控离体条件下评估了黄花蒿的反应。共有八组植物接受了 AgNPs 和紫外线 B 的单独/联合处理。为此，合成了球形且平均大小为 31.8 nm 的 AaAgNPs。分别计算了 0.5 mg L-1 AaAgNPs 单独处理和 0.5 mg L-1 AaAgNPs 与 3 h UV-B 联合处理时光合色素的最大值。结果表明，AaAgNPs 和紫外线-B 的共同暴露显著平衡了环斑金枪鱼的 ROS 生成，并提高了抗氧化酶的活性。荧光和扫描电子显微镜（SEM）分析表明，在 AaAgNPs 和 3 小时紫外线-B 的联合处理下，腺毛（GT）的面积和密度都有所增加。根据显微镜下 GT 结果之间的相关性，在使用低浓度 AaAgNPs 和紫外线-B 处理的 A. annua 植物中发现了高浓度的青蒿素和相关转录本的上调。因此可以推断，两种不同的植物生长调节剂，即低浓度生物合成的 AgNPs 和短期紫外线-B 照射，可以协同提高青蒿的生理生化特性和次生代谢产物（特别是青蒿素）的产量。

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引用次数: 0

Nano-priming: Improving plant nutrition to support the establishment of sustainable agriculture under heavy metal stress 纳米填料：改善植物营养，支持在重金属胁迫下建立可持续农业

Plant Nano Biology

Pub Date : 2024-10-11 DOI: 10.1016/j.plana.2024.100096

Mohammad Faizan , Pooja Sharma , Haider Sultan , Pravej Alam , Shafaque Sehar , Vishnu D. Rajput , Shamsul Hayat

Heavy metals (HMs) have become a severe problem for all living organisms, including plants, because of their unprecedented bioaccumulation and biomagnification in the environment. When exposed to hazardous quantities of HMs, various essential cellular macromolecules, including DNA and nuclear proteins, can interact with HMs, causing an overproduction of reactive oxygen species (ROS). Recently, several techniques have been used to ameliorate HM toxicity, including nano-priming, which effectively modulates plant physiological and biochemical responses under HM stress. This review summarizes the literature on the effectiveness of nano-priming for boosting germination, growth, photosynthetic efficiency, biomass accumulation, and crop yield. Additionally, information regarding the application of nano-priming to reduce HM toxicity in plants is reviewed. Future research prospects are indicated by highlighting the knowledge gaps in the current literature and underlining the need optimize and validate nano-priming techniques and their physiological effects on plants.

重金属（HMs）因其在环境中前所未有的生物蓄积性和生物放大性，已成为包括植物在内的所有生物体面临的一个严重问题。当暴露在有害的 HMs 中时，各种重要的细胞大分子（包括 DNA 和核蛋白）会与 HMs 相互作用，导致过量产生活性氧（ROS）。最近，有几种技术被用于改善 HM 的毒性，包括纳米引物，它能有效调节 HM 胁迫下的植物生理和生化反应。本综述总结了有关纳米引物在促进发芽、生长、光合效率、生物量积累和作物产量方面有效性的文献。此外，还综述了有关应用纳米填料降低植物 HM 毒性的信息。通过强调当前文献中的知识空白，并强调优化和验证纳米填料技术及其对植物生理效应的必要性，指出了未来的研究前景。

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引用次数: 0

Fabrication of cobalt oxide nanosheets using Withania somnifera root extract for degradation of organic pollutants 利用睡茄根提取物制备用于降解有机污染物的氧化钴纳米片

Plant Nano Biology

Pub Date : 2024-10-11 DOI: 10.1016/j.plana.2024.100099

Chinky Gangwar , Saloni Sahu , Ritik Jaiswal , Nisha Gangwar , Ashish Soni

In today’s scenario, material science emerges as pivotal players, as it is an emerging and fast-growing interdisciplinary field. Nanomaterials are increasingly popular among researchers due to their unique physicochemical properties and multifaceted applications. Recently, researchers are exploring more sustainable approaches for the synthesis of nanomaterials due to its numerous advantages. In this context, present investigation reports an approach for the synthesis of cobalt oxide nanosheets (Co₃O₄ NSs). This approach leverages environmentally friendly and sustainable methods, minimizing the use of hazardous chemicals and reducing energy consumption. Hence it involved Withania somnifera (ashwagandha) root extract as a greener reductant as well as stabilizing agent. The synthesized Co₃O₄ NSs were thoroughly analyzed using various techniques, including ultraviolet-visible (UV–vis) spectroscopy, fourier-transform infrared spectroscopy (FTIR), powder x-ray diffraction (PXRD), and field emission scanning electron microscopy (FE-SEM). A sharp absorption peak at 252 nm with a tail towards higher wavelength reveal the formation of Co₃O₄ NSs. The diffraction pattern reveals a face centered cubic structure of Co₃O₄ NSs. Morphological studies confirmed the substantial surface area of Co₃O₄ NSs which enable us to perform the catalytic degradation of azo dye, i.e., methyl orange. It provides that 10 mg of Co₃O₄ NSs is sufficient to degrade a 10 ppm aqueous methyl orange solution by 75.82 % in the dark and by 96.12 % under sunlight exposure. Thus, this study offers an excellent pathway for the synthesis of Co₃O₄ NSs and demonstrates their potential as a promising material for future catalytic applications.

在当今形势下，材料科学成为一个新兴的、快速发展的跨学科领域，其地位举足轻重。纳米材料因其独特的物理化学特性和多方面的应用，越来越受到研究人员的青睐。最近，由于纳米材料具有众多优点，研究人员正在探索更多可持续的纳米材料合成方法。在此背景下，本研究报告了一种合成氧化钴纳米片（Co3O4 NSs）的方法。这种方法利用了环保和可持续的方法，最大限度地减少了有害化学品的使用，降低了能耗。因此，它采用了睡莲（Withania somnifera）根提取物作为更环保的还原剂和稳定剂。利用紫外-可见（UV-vis）光谱、傅立叶变换红外光谱（FTIR）、粉末 X 射线衍射（PXRD）和场发射扫描电子显微镜（FE-SEM）等多种技术对合成的 Co3O4 NSs 进行了深入分析。在 252 纳米波长处有一个尖锐的吸收峰，其尾部向更高波长延伸，揭示了 Co3O4 NSs 的形成。衍射图样显示了 Co3O4 NSs 的面心立方结构。形态学研究证实，Co3O4 NSs 具有很大的表面积，使我们能够对偶氮染料（即甲基橙）进行催化降解。研究表明，10 毫克 Co3O4 NSs 就足以在黑暗条件下降解 10 ppm 的甲基橙水溶液 75.82%，在阳光照射下降解 96.12%。因此，这项研究为 Co3O4 NSs 的合成提供了一个很好的途径，并证明了其作为一种有前途的材料在未来催化应用中的潜力。

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引用次数: 0

Functional and transcriptomic insights into plant response to arginine-functionalized nanohydroxyapatite treatment 植物对精氨酸功能化纳米羟基磷灰石处理反应的功能和转录组学见解

Plant Nano Biology

Pub Date : 2024-10-09 DOI: 10.1016/j.plana.2024.100093

Chinenye L. Izuegbunam, Beate Wone, Bernard W.M. Wone

The manipulation of the plant genome is essential for elucidating gene functions in plants and advancing the development of climate-resistant crops. We have demonstrated that a nanohydroxyapatite (nHA)-mediated gene delivery system is effective in the transformation of reporter genes into six plant species. Despite the potential advantages of nHA-mediated biomolecule delivery and its application as fertilizers, phytotoxicity concerns necessitate additional studies. While initial findings suggest the beneficial effects of nHA as a nanofertilizer at specific concentrations, a thorough investigation into its bioactivity is warranted. This study reports the bioactivity of nHA on two model plants, including a crop species, and examines the global gene expression alterations in Arabidopsis thaliana. Treatment of seeds and seedlings with arginine-functionalized nHA (R-nHA) at concentrations at 50, 200 and 500 µg/ml led to accelerated germination in Arabidopsis, an effect not observed in Nicotiana benthamiana. Additionally, R-nHA did not affect root growth in either model species but significantly promoted root and leaf growth in Triticum aestivum. Transcriptomic analysis revealed minimal transcriptional changes in Arabidopsis treated with R-nHA compared to a water control, including activated phytohormone signaling pathways and stress-responsive genes. Salicylic acid (SA) has been identified as a pivotal phytohormone in initiating stress resistance in response to R-nHA exposure in Arabidopsis, highlighting its essential role in plant defense mechanisms against both biotic and abiotic stresses. In summary, this study showed that R-nHA accelerates germination and promotes plant growth with minimal transcriptional changes, thereby laying the groundwork for the use of nHA in plant genome manipulations. This research indicates that nHAs are highly biocompatible for plant bionanotechnology applications.

操纵植物基因组对于阐明植物基因功能和推动耐气候作物的开发至关重要。我们已经证明，纳米羟基磷灰石（nHA）介导的基因递送系统能有效地将报告基因转化到六种植物中。尽管纳米羟基磷灰石介导的生物分子递送及其作为肥料的应用具有潜在优势，但植物毒性问题仍需要进一步研究。虽然初步研究结果表明，在特定浓度下，nHA 作为纳米肥料具有有益作用，但仍有必要对其生物活性进行深入研究。本研究报告了 nHA 对两种模式植物（包括一种作物物种）的生物活性，并考察了拟南芥的全局基因表达变化。用浓度为 50、200 和 500 µg/ml 的精氨酸功能化 nHA（R-nHA）处理拟南芥的种子和幼苗，可加速其萌发，但在拟南芥中未观察到这种效应。此外，R-nHA 不会影响两种模式物种的根系生长，但会显著促进小麦的根系和叶片生长。转录组分析表明，与水对照相比，用 R-nHA 处理的拟南芥转录变化极小，包括激活的植物激素信号通路和胁迫响应基因。水杨酸（SA）已被确定为拟南芥对 R-nHA 暴露产生抗逆反应的关键植物激素，突出了它在植物防御机制中对生物和非生物胁迫的重要作用。总之，这项研究表明，R-nHA 能加速植物萌发并促进植物生长，而转录变化极小，从而为在植物基因组操作中使用 nHA 奠定了基础。这项研究表明，nHA 具有高度的生物兼容性，可用于植物仿生技术。

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引用次数: 0

Elucidation of biochemical and physiological modulations in Triticum aestivum induced by green synthesized nitrogen-enriched zinc nano-complexes 阐明绿色合成富氮纳米锌络合物诱导的小麦生化和生理变化

Plant Nano Biology

Pub Date : 2024-10-04 DOI: 10.1016/j.plana.2024.100094

Zari Shiran , Sedigheh Esmaeilzadeh Bahabadi , Zohreh Razmara , Kavitha Beluri , Nusrat Easmin , Amirhossein Mahdaviarab , Hamidreza Sharifan

This study investigates the efficacy of a green synthesized nitrogen-rich zinc complex (Zn-NC) using quinoline (C₉H₇N) as the nitrogen-rich substrate to enhance growth and biochemical properties in wheat (Triticum aestivum). The performance of Zn-NC was compared to standard zinc oxide nanoparticles (ZnO-NPs). Both Zn-NC and ZnO-NPs were characterized using scanning electron microscopy (SEM) and X-ray diffraction (XRD), and dynamic light scattering (DLS). Three concentrations (100, 200, and 500 ppm) of each compound, along with a control, were applied to local soil samples (n=3). The physiological (biomass, elongation) and biochemical effects (chlorophyll, carotenoids, flavonoids, and phenols) on wheat were investigated. Potential phytotoxic effects were evaluated to establish the biostimulants' safety thresholds. Plants treated with green Zn-NC showed an average increase in shoot length of 25 % compared to the control group. The chlorophyll content in plants treated with ZnO-NPs increased by 18 %, while those treated with green Zn-NC increased by 12 % compared to control. Application of ZnO-NPs resulted in a 30 % increase in total yield, whereas green Zn-NC treatment led to a 22 % yield increase. The root biomass of plants treated with ZnO-NPs increased by 28 %, and those treated with green Zn-NC saw a 20 % increase compared to controls. Based on the optimization of overall results, the ZnO NPs showed phytotoxic effects at concentrations above 200 ppm, while green Zn-NC exhibited no significant phytotoxicity even at concentrations up to 300 ppm. This study delineates the optimal concentrations of Zn-NC and ZnO-NPs that can enhance nutrient delivery and yield in cereal crops while mitigating phytotoxic risks. The findings provide valuable insights into applying nano-biostimulants in agroecosystems, highlighting their potential to improve productivity and sustainability in agriculture.

本研究探讨了以喹啉（C9H7N）为富氮底物的绿色合成富氮锌复合物（Zn-NC）对提高小麦（Triticum aestivum）生长和生化特性的功效。Zn-NC 的性能与标准氧化锌纳米颗粒（ZnO-NPs）进行了比较。使用扫描电子显微镜（SEM）、X 射线衍射（XRD）和动态光散射（DLS）对 Zn-NC 和 ZnO-NPs 进行了表征。每种化合物的三种浓度（100、200 和 500 ppm）与对照组一起施用于当地土壤样本（n=3）。研究了小麦的生理效应（生物量、伸长率）和生化效应（叶绿素、类胡萝卜素、类黄酮和酚类）。对潜在的植物毒性效应进行了评估，以确定生物刺激剂的安全阈值。与对照组相比，用绿色 Zn-NC 处理过的植物的嫩枝长度平均增加了 25%。与对照组相比，使用 ZnO-NPs 处理的植物叶绿素含量增加了 18%，而使用绿色 Zn-NC 处理的植物叶绿素含量增加了 12%。施用 ZnO-NPs 可使总产量增加 30%，而绿色 Zn-NC 处理可使产量增加 22%。与对照组相比，使用 ZnO-NPs 处理的植物根部生物量增加了 28%，而使用绿色 Zn-NC 处理的植物根部生物量增加了 20%。根据对总体结果的优化，氧化锌氮氧化物在浓度超过 200 ppm 时会产生植物毒性，而绿色 Zn-NC 即使在浓度达到 300 ppm 时也不会产生明显的植物毒性。这项研究确定了 Zn-NC 和 ZnO-NPs 的最佳浓度，既能提高谷类作物的养分输送和产量，又能降低植物毒性风险。研究结果为在农业生态系统中应用纳米生物刺激剂提供了宝贵的见解，凸显了纳米生物刺激剂在提高农业生产力和可持续性方面的潜力。

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引用次数: 0

Microbial Nanotechnology for Plant Science and Agriculture 植物科学与农业微生物纳米技术

Plant Nano Biology

Pub Date : 2024-08-01 DOI: 10.1016/j.plana.2024.100088

Hena Dhar, Javaid Akhter Bhat, Ulhas Kadam, Rupesh Deshmukh

引用次数: 0