Plant Nano Biology最新文献_第8页

Nanoparticles and polymer-based nanoparticles in plants and microbes 植物和微生物中的纳米颗粒和聚合物基纳米颗粒

IF 7.7

Plant Nano Biology

Pub Date : 2025-08-01 Epub Date: 2025-06-18 DOI: 10.1016/j.plana.2025.100165

Santosh Kumar, Seena Sahadevan, Sundararajan V. Madihally, Krishnakumar Balu

引用次数: 0

Revolutionizing crop production with iron nanoparticles for controlled release of plant growth regulators and abiotic stress resistance 革命性的作物生产与铁纳米颗粒控制释放植物生长调节剂和非生物抗逆性

Plant Nano Biology

Pub Date : 2025-08-01 Epub Date: 2025-07-11 DOI: 10.1016/j.plana.2025.100172

Sehar Razzaq , Beibei Zhou

Iron nanoparticles (Fe-NPs) have emerged as a revolutionary tool for enhancing the efficiency of plant growth regulators (PGRs) delivery in modern agriculture. This review explores how Fe-NPs address critical challenges in conventional PGR applications, including instability, rapid degradation, and non-target effects. Their unique properties, such as high surface area, magnetic responsiveness, and biocompatibility, enable the precise encapsulation and controlled release of key PGRs, including auxins, gibberellins, cytokinins, and abscisic acid, thereby improving bioavailability and reducing environmental contamination. Fe-NPs demonstrate remarkable potential in enhancing plant growth, stress tolerance (including drought and salinity), and crop productivity through targeted delivery mechanisms. Additionally, their dual role as both PGR carriers and iron micronutrient supplements offers synergistic benefits for plant health. While promising, challenges in scalability, cost-effectiveness, and environmental safety must be addressed for widespread adoption. By integrating nanotechnology with precision agriculture, Fe-NPs-mediated PGR delivery offers a sustainable approach to enhancing crop performance and resilience in the face of climate change and increasing global food demands. The objectives of this review are to highlight current advancements, key mechanisms involved in the target delivery of Fe-NPs, abiotic stress tolerance (including oxidative stress modulation and enhanced metabolic processes), applications, and future directions for harnessing Fe-NPs in next-generation agricultural practices.

铁纳米颗粒（Fe-NPs）已成为提高现代农业中植物生长调节剂（pgr）输送效率的革命性工具。这篇综述探讨了Fe-NPs如何解决传统PGR应用中的关键挑战，包括不稳定性、快速降解和非目标效应。它们的独特特性，如高表面积、磁响应性和生物相容性，使其能够精确封装和控制释放关键的pgr，包括生长素、赤霉素、细胞分裂素和脱落酸，从而提高生物利用度并减少环境污染。Fe-NPs在促进植物生长、抗逆性（包括干旱和盐度）和作物生产力方面具有显著潜力。此外，它们作为PGR载体和铁微量营养素补充剂的双重作用为植物健康提供了协同效益。虽然前景光明，但要广泛采用，必须解决可扩展性、成本效益和环境安全性方面的挑战。通过将纳米技术与精准农业相结合，fe - nps介导的PGR提供了一种可持续的方法，可以在面对气候变化和全球粮食需求不断增加的情况下提高作物性能和抵御能力。本文综述了Fe-NPs靶向递送的主要机制、非生物胁迫耐受性（包括氧化应激调节和增强的代谢过程）、应用以及在下一代农业实践中利用Fe-NPs的未来方向。

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引用次数: 0

Nano-improved plant salinity tolerance: The importance of K+/Na+ homeostasis and crosstalk between Ca2+ and hormones 纳米提高植物耐盐性：K+/Na+稳态和Ca2+与激素间串扰的重要性

IF 7.7

Plant Nano Biology

Pub Date : 2025-08-01 Epub Date: 2025-09-18 DOI: 10.1016/j.plana.2025.100196

Ibrahim A.A. Mohamed , Mohamed Frahat Foda , Irfan Ullah Khan , Maria Batool , Eman F.A. Awad-Allah , Chenjie Fan , Chengcheng Fu , Jie Wang , Zujun Yin , Honghong Wu

Salinity stress is a major constraint on plant organ morphogenesis, and agricultural production, mostly by disrupting ion homeostasis and plant water status, leading to detrimental K⁺/Na⁺ imbalance. Maintaining subcellular ionic balance is a critical defense mechanism against abiotic stresses, and plants employ diverse strategies to mitigate ion toxicity. Nanobiotechnology offers a promising approach to enhance plant ion homeostasis under stressed environments, leveraging nanoparticles' (NPs) capacity to modulate stress-responsive signaling pathways in crops. Crucially, NPs initiate crosstalk between Ca²⁺ signaling and hormonal networks, which cooperate with reactive oxygen species (ROS), K⁺, and nitric oxide (NO) signaling to regulate transcription factors (TFs) essential for ionic equilibrium. This review examines the role of NPs in promoting K⁺/Na⁺ homeostasis during salinity stress by regulating molecular, physiological, anatomical, and morphological mechanisms. These NP-induced Ca²⁺/hormonal networks directly or indirectly regulate NO signaling to bolster organ morphogenesis and stress tolerance. NPs enhance salinity tolerance by upregulating key genes (e.g., SOS1, SOS2, SOS3, HKT1, NHX), improving ion homeostasis and organ development. Moreover, NP-triggered crosstalk between Ca²⁺ signaling and hormones plays a pivotal role in regulating TFs such as bHLH, R2R3-MYB, WRKY, NAC, ZIP, ERFs, and NFX1. Collectively, these signaling and TF networks orchestrated by NPs sustain a high K⁺/Na⁺ ratio by regulating K⁺ and Ca²⁺ transport/distribution and reducing Na⁺ toxicity. Improved K⁺/Na⁺ regulation enhances nutrient uptake, activates ROS scavenging systems, modulates phytohormone levels, boosts photosynthetic efficiency, and optimizes stomatal motions. Understanding the mechanistic basis of NP-mediated stress regulation will elucidate their mode of action and the associated signaling cascades, clarifying their contribution to ion homeostasis under salinity stress.

盐胁迫是植物器官形态发生和农业生产的主要制约因素，主要是通过破坏离子稳态和植物水分状态，导致有害的K+/Na+失衡。维持亚细胞离子平衡是植物抵御非生物胁迫的重要防御机制，植物采用多种策略来减轻离子毒性。纳米生物技术提供了一种很有前途的方法来增强逆境环境下植物离子稳态，利用纳米颗粒（NPs）的能力来调节作物的应激反应信号通路。至关重要的是，NPs启动了Ca 2 +信号和激素网络之间的串扰，后者与活性氧（ROS）、K+和一氧化氮（NO）信号合作，调节离子平衡所必需的转录因子（tf）。本文综述了NPs通过调节分子、生理、解剖和形态机制促进盐胁迫下K + /Na +稳态的作用。这些np诱导的Ca + /激素网络直接或间接调节NO信号，增强器官形态发生和应激耐受性。NPs通过上调关键基因（如SOS1、SOS2、SOS3、HKT1、NHX）、改善离子稳态和器官发育来增强耐盐性。此外，np触发的Ca 2 +信号与激素之间的串扰在bHLH、R2R3-MYB、WRKY、NAC、ZIP、ERFs和NFX1等tf的调控中起着关键作用。总的来说，这些由NPs协调的信号和TF网络通过调节K +和Ca 2 +的运输/分布和降低Na +的毒性来维持高K + /Na +的比例。改进的K + /Na +调节增强了养分吸收，激活了ROS清除系统，调节了植物激素水平，提高了光合效率，优化了气孔运动。了解np介导的胁迫调节的机制基础将阐明它们的作用模式和相关的信号级联，阐明它们对盐胁迫下离子稳态的贡献。

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引用次数: 0

Effects of graphene-derived nanomaterials on the early development of the C4 Poaceae Setaria italica 石墨烯衍生纳米材料对C4禾本科意大利狗尾草早期发育的影响

IF 7.7

Plant Nano Biology

Pub Date : 2025-08-01 Epub Date: 2025-07-30 DOI: 10.1016/j.plana.2025.100180

Wilfredo Rondan , Antony Cristhian Gonzales-Alvarado , Glêyce de Oliveira Ferreira , Nathalia de Setta , Ana Champi

The growing demand for sustainable agricultural solutions has driven the exploration of advanced graphene-derived nanomaterials (GDNs). This study evaluated the effects of graphite (Gr), multilayer graphene (MLG), and graphene oxide (GO) on the early performance of the C4 Poaceae model species Setaria italica. GDNs were synthesized and characterized using Raman spectroscopy, atomic force microscopy, zeta potential, and UV-Vis analysis to identify the presence of structural defects, functional -OH groups and thiol (-SH) groups, as part of the study of their physicochemical properties. To investigate the impact of graphene-derived nanomaterials on S. italica agronomic traits, we performed experiments using Gr, MLG, and GO as soil amendments. Plants were cultivated on four concentrations of Gr, MLG, and GO, and maintained at field capacity for 25 days. We then assessed germination and agronomic traits to evaluate the response of seedlings to these treatments. Principal component analysis and correlation matrices were performed to obtain an integrated profile of the responses to treatment with GDNs. Our results showed that GDNs treatments did not significantly affect the germination profile of S. italica in an agronomic context. On the other hand, root length and total height were improved with MLG and GO treatments, while stem height was increased in the Gr treatment. The presence of thiol and -OH functional groups at the edges or between layers of GO and MLG can be related to plant growth performance, highlighting the potential of GDNs as agricultural nanomaterials to enhance crop productivity and stress resilience, emphasizing the need to optimize material properties and dosages for targeted applications in precision agriculture.

对可持续农业解决方案日益增长的需求推动了对先进石墨烯衍生纳米材料（gdn）的探索。本研究评价了石墨（Gr）、多层石墨烯（MLG）和氧化石墨烯（GO）对C4禾本科模式物种意大利狗尾草（Setaria italica）早期生长性能的影响。利用拉曼光谱、原子力显微镜、zeta电位和UV-Vis分析对gdn进行了合成和表征，以确定结构缺陷、官能团-OH和硫醇（-SH）基团的存在，作为其物理化学性质研究的一部分。为了研究石墨烯衍生纳米材料对意大利葡萄农艺性状的影响，我们使用Gr、MLG和GO作为土壤改良剂进行了实验。植物在4种浓度的Gr、MLG和GO上培养，并保持田间容量25天。然后，我们评估了发芽和农艺性状，以评估幼苗对这些处理的反应。进行主成分分析和相关矩阵，以获得对gdn治疗反应的综合概况。结果表明，在农艺条件下，GDNs处理对意大利葡萄的萌发没有显著影响。另一方面，MLG和GO处理提高了根长和总高，而Gr处理提高了茎高。硫醇和-OH官能团在氧化石墨烯和MLG的边缘或层之间的存在可能与植物生长性能有关，突出了gdn作为农业纳米材料提高作物生产力和抗逆性的潜力，强调了优化材料性能和剂量的必要性，以实现精准农业的目标应用。

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引用次数: 0

Green synthesized nanoparticles for disease management in vegetable crops: A review 绿色合成纳米颗粒用于蔬菜作物病害管理：综述

IF 7.7

Plant Nano Biology

Pub Date : 2025-08-01 Epub Date: 2025-07-30 DOI: 10.1016/j.plana.2025.100179

Deepika Sharma , Ashutosh Sharma , Harender Raj Gautam

Vegetables are an important part of our diet and provide essential nutrients, minerals and vitamins that are beneficial to human health. Vegetables are known as protective foods as they promote the uptake of nutrients, fiber and other components. Present vegetable crop farming frequently depends on chemicals that result in environmental issues, including soil deterioration, pollution, and greenhouse gas emissions. The utilization of organic resources that can either fully or partially replace synthetic chemicals are needed to achieve crop protection and sustainability. A notable field in this context is "nanotechnology", which involves the creation, manipulation, and application of materials at the nanoscale that can be effectively utilized in plant disease management. The science of nanotechnology is concerned with the use of nanomaterials (10⁻⁹m in size) to combat plant diseases and improve plant defense mechanisms. The nanotechnology has significant potential to reduce the impact of environmental stresses due to chemicals. Nanoparticles can be synthesized using different approaches, however, nanoparticles produced using living organisms is beneficial, economical and environment friendly. Various, green-synthesized nanoparticles of silver, copper, iron, and zinc have antifungal activity and has the potential to attack plant infections by altering the permeability and respiratory activities of plant cells. The green nanoparticles and their utilization are being recognized and explored in agriculture. This review examines the efficacy of biological agents in the manufacture of eco-friendly nanoparticles utilizing various metallic ions to manage plant pests in vegetable crops. The focus of this review is mainly on the efficacy of green nanoparticles in controlling diseases affecting various vegetable crops. Future research perspectives are outlined to optimize the effectiveness of green nanotechnology in combating plant diseases.

蔬菜是我们饮食的重要组成部分，提供对人体健康有益的必需营养素、矿物质和维生素。蔬菜被称为保护性食物，因为它们促进营养、纤维和其他成分的吸收。目前的蔬菜作物种植经常依赖于化学品，导致环境问题，包括土壤恶化、污染和温室气体排放。为了实现作物保护和可持续性，需要利用能够完全或部分取代合成化学品的有机资源。在这方面一个值得注意的领域是“纳米技术”，它涉及在纳米尺度上创造、操作和应用材料，这些材料可以有效地用于植物病害管理。纳米技术科学涉及使用纳米材料（尺寸为10 - 9米）来对抗植物疾病和改善植物防御机制。纳米技术在减少化学物质造成的环境压力方面具有巨大的潜力。纳米颗粒的合成方法多种多样，但利用生物体生产纳米颗粒具有有益、经济、环保的特点。各种绿色合成的银、铜、铁和锌纳米颗粒具有抗真菌活性，并有可能通过改变植物细胞的渗透性和呼吸活动来攻击植物感染。绿色纳米颗粒及其在农业上的应用正在被认识和探索。本文综述了生物制剂在利用各种金属离子制备环保型纳米颗粒治理蔬菜作物害虫方面的效果。本文主要对绿色纳米颗粒防治蔬菜病害的效果进行了综述。展望了绿色纳米技术在植物病害防治中的应用前景。

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引用次数: 0

Different types of honey on the synthesis of silver nanoparticles (AgNPs) and their antibacterial activity: In-vitro and in-silico studies 不同类型蜂蜜对银纳米颗粒（AgNPs）合成及其抗菌活性的影响：体外和硅内研究

IF 7.7

Plant Nano Biology

Pub Date : 2025-08-01 Epub Date: 2025-09-02 DOI: 10.1016/j.plana.2025.100188

Saidun Fiddaroini , Kurnia Indu , Luailik Madaniyah , Suci Amalia , Aulanni'am , Moh. Farid Rahman , Akhmad Sabarudin

AgNPs exhibit significant antibacterial activity, which is enhanced by their nanoscale size. Green synthesis using honey offers an eco-friendly, straightforward approach, with glucose and fructose in honey playing key roles in AgNPs synthesis. This study explores the effects of glucose and fructose concentrations in various honey types on AgNPs formation at 27–30 °C and pH 6–6.5, complemented by molecular docking studies. The sugar content in different honey samples was as follows: Cottonwood (56.66 %), Rambutan (49.95 %), Rubber (44.54 %), and Coffee (37.56 %). Higher bioreductor concentrations led to increased absorbance in the UV-Vis spectra; however, antibacterial activity decreased, albeit not significantly. This can be attributed to lower reducing sugar concentrations, which resulted in smaller AgNPs with a larger surface area, consequently affecting their antibacterial efficacy. The synthesized AgNPs were spherical (8–10 nm) and exhibited face-centered cubic crystallinity. The inhibition zones for AgNPs derived from cottonwood, rambutan, rubber, and coffee honey against Staphylococcus aureus were 14.51 mm, 14.54 mm, 15.45 mm, and 16.04 mm, respectively, and against Pseudomonas aeruginosa were 15.10 mm, 15.70 mm, 15.81 mm, and 15.90 mm, respectively. The microdilution broth assay revealed a sharp increase in antibacterial inhibition within the AgNPs concentration range of 5–50 ppm, plateauing above 50 ppm, with the steep increase halting between 20 and 40 ppm. MIC values ranged from 11.47 to 13.37 ppm for S. aureus and 8.71–10.62 ppm for P. aeruginosa. Molecular docking studies confirmed that D-glucose and D-fructose bind to bacterial proteins PBP2a and PBP3, supporting their role as bioreductors in AgNPs formation.

AgNPs具有显著的抗菌活性，其纳米级尺寸增强了抗菌活性。蜂蜜中的葡萄糖和果糖在AgNPs合成中起着关键作用，利用蜂蜜进行绿色合成提供了一种环保、直接的方法。本研究探讨了不同蜂蜜中葡萄糖和果糖浓度在27-30 °C和pH 6-6.5条件下对AgNPs形成的影响，并进行了分子对接研究。不同蜂蜜样品的含糖量分别为杨木（56.66 %）、红毛丹（49.95 %）、橡胶（44.54 %）和咖啡（37.56 %）。较高的生物还原剂浓度导致紫外-可见光谱的吸光度增加；然而，抗菌活性下降，尽管不明显。这可归因于较低的还原糖浓度，导致AgNPs较小，表面积较大，从而影响其抗菌效果。合成的AgNPs为球形（8-10 nm），具有面心立方结晶度。杨木、红毛丹、橡胶和咖啡蜂蜜提取的AgNPs对金黄色葡萄球菌的抑制区分别为14.51 mm、14.54 mm、15.45 mm和16.04 mm，对铜绿假单胞菌的抑制区分别为15.10 mm、15.70 mm、15.81 mm和15.90 mm。微量稀释肉液试验显示，AgNPs的抑菌作用在5-50 ppm范围内急剧增加，在50 ppm以上达到稳定水平，在20 - 40 ppm之间停止急剧增加。金黄色葡萄球菌的MIC值为11.47 ~ 13.37 ppm，铜绿假单胞菌的MIC值为8.71 ~ 10.62 ppm。分子对接研究证实，d -葡萄糖和d -果糖与细菌蛋白PBP2a和PBP3结合，支持它们在AgNPs形成中作为生物还原剂的作用。

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引用次数: 0

Synergistic effects of zinc and silicon dioxide nanoparticles improve cucumber (Cucumis sativus L) drought tolerance 锌和二氧化硅纳米粒子协同作用提高黄瓜抗旱性

IF 7.7

Plant Nano Biology

Pub Date : 2025-08-01 Epub Date: 2025-09-11 DOI: 10.1016/j.plana.2025.100193

Luis Alonso Valdez-Aguilar , Daniela Alvarado-Camarillo , Ponciano Solórzano-Martínez , Luis Alfonso García-Cerda , Ileana Vera-Reyes

Water scarcity significantly threatens agricultural productivity because of changing precipitation patterns and increasing competition for water use. Nanotechnology presents a sustainable and cost-effective strategy to improve water use efficiency, particularly through the application of silicon dioxide nanoparticles (nSiO₂) and zinc (Zn), as they alleviate water stress by enhancing plant water relationships. This study assessed the effects of nSiO₂ alone or in combination with zinc oxide (ZnO) at concentrations of 1.5 % and 3.0 %, applied at 150 mg L⁻¹ , on cucumber plants under water stress. The results indicated that nSiO₂ and nSiO₂ + ZnO_1.5 % significantly increased fruit yield by 52.7 % (5134.3 g), whereas water stress reduced yield by 31.1 % (2449.7 g). These treatments, nSiO₂ and nSiO₂ + ZnO_1.5 %, helped recover fruit production under drought conditions, with yields reaching levels comparable to those of well-irrigated control plants. Moreover, they reduced fruit abortion by 27.1 % and 25.2 %, respectively. The application of nSiO₂ + ZnO_1.5 % and nSiO₂ + ZnO_3.0 % increased the root biomass under both normal and deficit irrigation and increased the root-to-shoot ratio, indicating adaptive biomass allocation to optimize water uptake. The net photosynthesis rate improved in drought-stressed plants treated with nSiO₂ alone, whereas under regular irrigation, the combination with ZnO_1.5 % was more effective. The mesophyll conductance decreased in drought-stressed plants treated with nSiO₂ + ZnO_1.5 %, which was associated with increased intrinsic water use efficiency (iWUE). The combination also increased leaf Zn levels and improved stomatal conductance, although nSiO₂ alone reduced the leaf silicon content, suggesting that the cultivar is not a silicon accumulator. The combination of nSiO₂ and ZnO_1.5 % is a promising approach to increase drought tolerance in cucumbers by improving yield, water use efficiency, and physiological responses under water-limited conditions

由于降水模式的变化和用水竞争的加剧，水资源短缺严重威胁着农业生产力。纳米技术提出了一种可持续的、具有成本效益的策略来提高水的利用效率，特别是通过应用二氧化硅纳米颗粒（nsio2）和锌（Zn），因为它们通过增强植物的水分关系来缓解水分胁迫。本研究评估了nsio2单独或与氧化锌（ZnO）在1.5 %和3.0 %浓度下，以150 mg L⁻¹ 的剂量施用对水分胁迫下黄瓜植株的影响。结果表明，nSiO₂和nSiO₂+ ZnO1.5 %处理显著提高了果实产量52.7 %(5134.3 g)，而水分胁迫使果实产量降低了31.1 %（2449.7 g）。nsio_2和nsio_2 + ZnO1.5 %处理有助于恢复干旱条件下的果实产量，产量达到与灌溉良好的对照植株相当的水平。此外，它们还能使果实败育率分别降低27.1% %和25.2% %。施用nSiO₂+ ZnO1.5 %和nSiO₂+ ZnO3.0 %均增加了正常和亏水灌溉下的根系生物量，提高了根冠比，表明生物量配置的适应性优化了水分吸收。单用nsio2处理能提高干旱胁迫植株的净光合速率，而在常规灌溉条件下，以ZnO1.5 %配合处理效果更好。nsio_2 + ZnO1.5 %处理降低了干旱胁迫植株的叶肉导度，提高了内在水分利用效率（iWUE）。尽管nsio2单独处理降低了叶片硅含量，但nsio2处理提高了叶片锌含量，改善了气孔导度，表明该品种不是硅积累者。nsio2和ZnO1.5 %的组合处理是一种很有希望通过提高产量、水分利用效率和水分限制条件下的生理反应来提高黄瓜抗旱性的方法

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引用次数: 0

The effect of biogenic silver nanoparticles on the germination and phenophase of soybean (Glycine max (L.) Merr.) var. Anjasmoro 生物源纳米银对大豆（Glycine max (L.)）萌发和物候期的影响先生)var. Anjasmoro

Plant Nano Biology

Pub Date : 2025-08-01 Epub Date: 2025-07-04 DOI: 10.1016/j.plana.2025.100170

Elah Nurlaelah , Windri Handayani , Ratna Yuniati , Ezza Syuhada Sazali

Silver nanoparticles (AgNPs) have gained considerable attention in agriculture for their potential to enhance plant growth and productivity. However, their application may also induce phytotoxic effects, depending on factors such as concentration, particle size, plant species, and mode of exposure. In this study, AgNPs were biosynthesized using Diospyros discolor leaf extract and applied to soybean (Glycine max (L.) Merr. var. Anjasmoro) to evaluate their effects on seed germination and plant development through nanopriming and foliar spray treatments. In the first experiment, soybean seeds were soaked with AgNPs at concentrations of 20, 40, and 60 mg/L. The effects on germination rate, shoot and root length, seed vigor index, and chlorophyll a and b content were evaluated on the seedlings after 7 days. In the second experiment, 20 mg/L AgNPs were applied through seed nanopriming, foliar spray application, and a combination of both methods, then their effects were evaluated up to 78 days after sowing and spraying. Their effects on plant height, leaf number, root length, biomass, chlorophyll content, phenological stages (flowering and fruiting time), and oxidative stress markers (hydrogen peroxide and total phenolic content) were assessed. The results showed that germination rates remained above 95 % across all AgNP treatments. Specifically, 20 mg/L AgNPs significantly enhanced shoot elongation and seed vigor, while 60 mg/L reduced root length. Chlorophyll a and b contents increased significantly at 40 and 60 mg/L, respectively. In mature plants, the combined application of nanopriming and foliar spray led to reductions in vegetative growth parameters, accompanied by elevated hydrogen peroxide and phenolic content, indicating oxidative stress. However, foliar and combined treatments effectively accelerated the flowering and fruiting phases. These findings demonstrate the dual role of biogenic AgNPs in stimulating physiological processes while potentially inducing oxidative stress, depending on the dose and application method.

银纳米颗粒（AgNPs）因其促进植物生长和生产力的潜力而在农业领域受到了相当大的关注。然而，它们的应用也可能引起植物毒性作用，这取决于诸如浓度、颗粒大小、植物种类和暴露方式等因素。本研究以脱色薯蓣叶提取物为原料合成AgNPs，并将其应用于大豆（Glycine max (L.)）稳定。评价纳米喷雾和叶面喷雾处理对种子萌发和植株发育的影响。在第一个实验中，大豆种子分别用浓度为20、40和60 mg/L的AgNPs浸泡。7 d后对幼苗发芽率、茎长和根长、种子活力指数、叶绿素a和b含量的影响进行评价。在第二个试验中，通过种子纳米喷施、叶面喷施以及两种方法联合施用20 mg/L AgNPs，并在播种和喷施后78 d对其效果进行评价。评估了它们对株高、叶数、根长、生物量、叶绿素含量、物候阶段（开花和结实时间）和氧化胁迫标志物（过氧化氢和总酚含量）的影响。结果表明，所有AgNP处理的发芽率均保持在95% %以上。其中，20 mg/L AgNPs显著提高了茎伸长和种子活力，60 mg/L AgNPs显著降低了根长。叶绿素a和b含量分别在40和60 mg/L时显著增加。在成熟植物中，纳米喷雾和叶面喷雾联合施用导致营养生长参数降低，同时过氧化氢和酚含量升高，表明氧化应激。而叶面处理和组合处理能有效地加速开花和结果阶段。这些发现证明了生物源性AgNPs在刺激生理过程的同时可能诱导氧化应激的双重作用，这取决于剂量和应用方法。

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引用次数: 0

The influence of iodine nano citrates on juvenile wheat plants at phytopathogenic infection and cooling stress 纳米柠檬酸碘对小麦幼苗病原菌侵染和降温胁迫的影响

IF 7.7

Plant Nano Biology

Pub Date : 2025-08-01 Epub Date: 2025-07-26 DOI: 10.1016/j.plana.2025.100175

Hanna Huliaieva , Iryna Tokovenko , Maksym Kharchuk , Mykhailo Bohdan , Lidiia Pasichnyk

Abiotic stress factors, including those caused by global climate change, can worsen crop damage from phytopathogenic microorganisms. Iodine, as an inorganic antioxidant in the form of iodine nanoparticles, can serve as an additional element to help maintain the redox balance of cells when used for nano-biofortification of plants. Therefore, our study aimed to evaluate the effect of iodine nanoparticles on changes in the H₂O₂ pool and leaf pigment composition in the plant-host-phytopathogen system after a sudden, short-term cold snap, along with a preliminary assessment of how treatment with these nanoparticles influences growth parameters of both healthy and infected wheat plants. Notably, a sudden cold snap increased oxidative stress in tissues of young wheat plants affected by phytoplasmas and bacteria, as evidenced by a significant rise in hydrogen peroxide content in leaf tissues and pigment degradation. An increase in H₂O₂ levels in leaf tissues was also observed following the cooling stress in plants that received pre-sowing treatment with iodine nanoparticles. Iodine treatment may enhance the sensitivity of chloroplasts to oxidative stress and act as an antioxidant, triggering the antioxidant system, thereby helping to stabilize the cellular condition.

非生物胁迫因素，包括由全球气候变化引起的胁迫因素，可以加重植物病原微生物对作物的损害。碘作为一种无机抗氧化剂，以碘纳米颗粒的形式存在，当用于植物的纳米生物强化时，可以作为一种额外的元素来帮助维持细胞的氧化还原平衡。因此，我们的研究旨在评估在突如其来的短期寒流后，碘纳米颗粒对植物-宿主-植物病原体系统中H2O2池和叶片色素组成变化的影响，并初步评估这些纳米颗粒处理如何影响健康和感染小麦植株的生长参数。值得注意的是，突如其来的寒流增加了受植物浆体和细菌影响的小麦幼体组织中的氧化应激，这可以从叶片组织中过氧化氢含量和色素降解的显著增加中得到证明。在播种前用碘纳米颗粒处理的植物，叶片组织中H2O2水平也在降温胁迫后升高。碘处理可以增强叶绿体对氧化应激的敏感性，并起到抗氧化剂的作用，触发抗氧化系统，从而有助于稳定细胞状况。

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引用次数: 0

Nanoparticle-driven modulation of DREB/CBF transcription factors enhances lead phytoremediation in diverse plant species 纳米颗粒驱动的DREB/CBF转录因子调控增强了不同植物物种的铅修复

IF 7.7

Plant Nano Biology

Pub Date : 2025-08-01 Epub Date: 2025-09-03 DOI: 10.1016/j.plana.2025.100189

Fazal Hussain , Fazal Hadi , Nasir Ali

Lead (Pb) contamination in the environment poses a significant threat to plant health and ecosystem stability, necessitating advanced strategies to enhance phytoremediation efficacy. In this study, we investigated the potential of foliar-applied nanoparticles (NPs) to modulate stress-responsive transcription factors (DREB1A, DREB1B, DREB1F, and CBF) and biochemical pathways, thereby improving Pb tolerance and accumulation in Cannabis sativa, Ricinus communis, and Parthenium hysterophorus. Plants were subjected to Pb stress (200 ppm) and treated with copper, iron, magnesium, manganese, molybdenum, or zinc NPs (15 ppm), followed by a comprehensive evaluation of genomic responses, biochemical markers, and Pb uptake. Our findings reveal species- and NP-specific regulatory mechanisms governing Pb stress adaptation. Copper and molybdenum NPs markedly up regulated DREB1A and CBF expression in R. communis and C. sativa, correlating with increased proline accumulation (R² = 0.95), phenolic content, and Pb uptake. Molybdenum NPs facilitated the highest Pb accumulation in R. communis (0.63 ± 0.02 mg/g), whereas manganese NPs maximized Pb uptake in C. sativa (0.61 ± 0.05 mg/g). In contrast, P. hysterophorus exhibited minimal DREB1F induction but significant CBF activation under iron NP treatment, leading to Pb accumulation of 0.54 ± 0.05 mg/g. Biochemical analyses demonstrated strong correlations (R² = 0.99) between stress metabolite synthesis and transcriptional regulation, reinforcing the role of NPs in modulating molecular responses to Pb stress. These findings endorse the prime role of nanoparticle-mediated gene activation in enhancing phytoremediation efficiency. By integrating molecular and biochemical insights, this study provides a framework for species-specific NP applications to optimize eco-friendly remediation strategies for heavy metal-contaminated environments.

环境铅污染严重威胁植物健康和生态系统稳定，需要先进的修复策略来提高植物修复效果。在这项研究中，我们研究了叶面施用纳米颗粒（NPs）调节应激响应转录因子（DREB1A, DREB1B， DREB1F和CBF）和生化途径的潜力，从而提高大麻，蓖麻和子宫Parthenium hysterophorus的Pb耐受性和积累。将植物置于Pb胁迫（200 ppm）和铜、铁、镁、锰、钼或锌NPs（15 ppm）处理下，然后对基因组响应、生化标记和Pb摄取进行综合评估。我们的研究结果揭示了物种和np特异性的铅胁迫适应调节机制。铜和钼NPs显著上调了红豆和红花中DREB1A和CBF的表达，与脯氨酸积累（R²= 0.95）、酚类含量和铅吸收增加相关。钼NPs促进了红豆对Pb的最大积累（0.63 ± 0.02 mg/g），而锰NPs则促进了红豆对Pb的最大吸收（0.61 ± 0.05 mg/g）。相比之下，铁NP处理下，子宫草对DREB1F的诱导作用最小，但对CBF的激活作用显著，Pb积累量为0.54 ± 0.05 mg/g。生化分析表明，胁迫代谢物合成与转录调控之间存在很强的相关性（R²= 0.99），这进一步证实了NPs在Pb胁迫下的分子调控作用。这些发现证实了纳米颗粒介导的基因激活在提高植物修复效率方面的主要作用。通过整合分子和生物化学的见解，本研究为物种特异性NP应用提供了一个框架，以优化重金属污染环境的生态修复策略。

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