Vacunas (English Edition)最新文献

Introduction

The increasing prevalence of Neisseria gonorrhoeae has become a significant global concern. Consequently, it is of utmost importance to explore prophylactic approaches to combat the anti-microbial resistance in N. gonorrhoeae.

Objective

The present study aims to design a multi-epitopic vaccine construct using immunoinformatics and pan-genomic methodology.

Methods

The initial phase involves retrieving and re-annotating the 133 complete genome sets of N. gonorrhoeae. Subsequently, a pan-genome analysis was conducted to identify the core genes, followed by gene mapping. Non-homologous outer-membrane proteins were filtered out and analyzed using various epitope prediction algorithms targeting major histocompatibility complex (MHC-I, MHC-II), and B cells. The optimal epitopes were selected based on immunogenicity, antigenicity, toxicity, and solubility. Then, the vaccine constructs were designed using different combinations of linkers, Histidine (His) tags, adjuvants, and the finalized epitopes.

Results

The vaccine construct, V13 was screened as the most suitable candidate based on its physiochemical and antigenicity properties. Computational techniques assessed the efficacy of V13 against different immune receptors supported by immune simulation, indicating its safety for inducing immune responses against N. gonorrhoeae.

Conclusion

The chimeric multi-epitopic vaccine V13 construct can potentially trigger a diverse array of protective immune responses and serve as a promising starting point for future experimental investigations.

The world has successfully overcome the menace of Coronavirus disease-19 (COVID-19) pandemic waves, nature has unleashed a new curveball in the years 2022 and 2023 with other highly infectious viral diseases. Understanding the origin and different transmission routes, evolution, the mechanism of their emergence, immune evasion, and vaccine research while dealing with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has advanced our scientific knowledge and public health infrastructure to address unknown future viral pathogens already underway. The current review briefly discussed the worldwide pandemics of Monkeypox (Mpox) and polio viral infection along with the origin theories of other global viral outbreaks post-COVID-19 era in 2022 and 2023 like Ebola, unexplained hepatitis in pediatric children, avian influenza, and Langya virus. The role of climate, biodiversity, zoonotic transmission, and trajectory of these viral infections. It also highlights the containment, preventive, and treatment strategies that are being developed.

COVID-19 pandemic has challenged the National Regulatory Agencies (NRAs). One of these challenges is the transfer and validation of new vaccine methodologies so that they are safe, quality, and effective in protecting the population. In this article, we review the process that the Mexican NRA followed for the Vaxzevria and Convidecia vaccines; where communication between manufacturers and NRA allowed the methodological transfer and validation to be carried out before the first batch for application was ready in compliance with WHO guidelines. In this way, the importance of the relationship that must exist between manufacturers and NRAs is manifested to provide an immediate response and provide the population with a means of protection in the event of a public health emergency.

Communication between health professionals and patients remains a challenge from the point of view of health education and health care. Training in patient–professional communication techniques and improving the communication skills of healthcare professionals is of particular interest. This document aims to share with the healthcare professional community the “Decalogue of good practices in vaccine communication between immunocompromised patients and healthcare professionals”. A QR code is included at the end of the document where the Decalogue can be downloaded for its use and distribution.

The equitable distribution of vaccines for infectious diseases in low- and middle-income countries (LMICs) has become a hotly debated topic among public professionals and Government The decisions made in this regard can have far-reaching consequences for the health and well-being of millions of people including general population and healthcare professionals. There is need toconsider a fair and just distribution system to ensure that vaccines are provided to those who need them the most. For instance, Hepatitis B virus (HBV), influenza, and COVID-19 are crucial for healthcare professionals as they play in the fight against infectious disease outbreaks. However, in LMICs, poor vaccine confidence and uptake rates represent substantial hurdles for healthcare practitioners in obtaining and administering vaccines. Further, equitable vaccine uptake is a debatable policy matter in LMICs. These challenges stem from doubts regarding vaccine efficacy and safety, inaccurate information, and a lack of trust in government and medical institutions. Addressing misconceptions and building confidence, robust monitoring and evaluation mechanisms requires to ensure the safety of vaccines. . This review highlighted the importance of enhancing vaccine uptake motivation among health workers. Moreover, enhance the the need to respect individual choice when refuse. Ultimately, building confidence in the healthcare system is critical for addressing healthcare providers' reluctance about vaccines. The transtheoretical model is extremely useful for for understanding the behaviours and factors increasing vaccine uptake notably for infection prevention against hepatitis B, COVID-19, and influenza. The immplication of framework provided a holistic approach by customizing solutions to each stage of behavior change, from awareness to continuing support. Emphasizing education, incentives, and a supportive environment promotes a resilient healthcare system, allowing health workers community to actively contribute to public health through effective infection control.

Nanocarrier-based formulations such as niosomes are experiencing a rise in popularity owing to their substantial benefits over conventional formulations. Niosomes, which are novel vesicular systems, are composed mainly of hydrated amphiphilic molecules encompassing a variety of non-ionic surfactant classes. They have since emerged as a significant novel drug delivery system employed by numerous research groups for sustained and targeted deliveries. As well as conventional oral and parenteral administrations, biotechnological products can also be administered via transdermal, intranasal, ocular, and vaginal routes. The use of niosomes for vaccine delivery offers an interesting and promising area of research in the field of drug delivery. Furthermore, niosomes have demonstrated their efficacy as immunoadjuvants. This overview provides an extensive viewpoint on the emerging applications of niosomes in vaccine delivery which is a milestone in the immune-providing industry. A wide range of surfactants with diverse polar head groups, in addition to 1, 2, or 3 lipophilic alkyls, perfluoroalkyl, and steroidal moieties, can be used to generate the appropriate vesicular structures for encapsulating both hydrophilic and hydrophobic substances. The techniques utilized for niosome preparation, aspects related to the stability of vesicles and the administration routes for these amphiphilic assemblies are also deliberated upon.

The emergence of the novel coronavirus-based illness, COVID-19, in late 2019 marked a watershed moment in modern history. Initially identified in Wuhan, China, this viral epidemic swiftly transformed into a global pandemic, affecting worldwide. The World Health Organization (WHO) officially labeled the virus as “Severe Acute Respiratory Syndrome Coronavirus 2” (SARS-CoV-2) and the ensuing disease as Coronavirus Disease 2019 (COVID-19) on February 11, 2020. The spectrum of effects induced by COVID-19 encompasses a diverse array of conditions, ranging from sensory disturbances like anosmia and ageusia to more intricate manifestations, including headaches and, alarmingly, multiorgan failure and neuromuscular dysfunction, and even deaths were reported. COVID-19 triggers a vigorous inflammatory response characterized by the overproduction of pro-inflammatory cytokines. “Cytokine storm” significantly underwrites the worsening of the condition of patients, resulting in abnormalities such as ARDS. The major reason behind mortality from COVID-19 is respiratory failure instigated by ARDS. Throughout the early respiratory contamination, SARS-CoV-2 was hypothesized to target type-II pneumocytes lining the respiratory passage that specifically expressed TMPRSS2 and ACE2 receptors. The extra-pulmonary consequences of COVID-19 have been linked to the occurrence of these receptors in the skeletomuscular and brain tissues. As a result, it is condemning to comprehend the unique pathophysiological mechanisms behind the virus's manner of invasion in the tissues and also the disease's consequences. In this article, we focus on the complications caused by COVID-19 on musculoskeletal and neural tissues, the route of invasion, and the future perspective of dealing with the receptors whose expression leads to COVID-19 infection.

Objective

This research aimed to assess the incidence of neurological complication symptoms among residents of the Makkah province in Saudi Arabia following the rapid approval and administration of mRNA vaccines. Given the differences in demographic factors such as age, race, medical history, and lifestyle among individuals, close monitoring of vaccine-related side effects becomes crucial, especially in mass vaccination campaigns.

Method

A cross-sectional online survey study was conducted in both English and Arabic languages, with a sample size determined using the Raosoft sample size calculator statistical analyzer. The study aimed for a 95% confidence level, a 5% margin of error, and a response distribution of 50%. Descriptive statistics were employed using Statistical Package for Social Sciences version 22.0. The Shapiro–Wilk test was utilized to assess data normality, and the statistical significance of the data was determined using Pearson's Chi-Squared test and the Kruskal–Wallis test.

Result

The study included 846 volunteers aged 18 and above, all of whom had received mRNA vaccine in the Makkah region. Data were systematically tabulated and categorized for descriptive analysis. The study found P-values less than .05, and the summarized P-values were presented. The majority of participants (73%) were from the Taif area (32%) within the Makkah region.

Conclusion

This study sheds light on the challenges faced by the population of the Makkah region in Saudi Arabia following mRNA vaccinations. Although some complications were reported post-vaccination, it is noteworthy that these challenges were effectively managed by the medical authorities.