Pub Date : 1988-11-01DOI: 10.1016/S0176-6724(88)80163-9
K. Roszkowski , H.L. Ko , J. Beuth , Y. Ohshima , W. Roszkowski , J. Jeljaszewicz , G. Pulverer
BALB/c-mice were treated for 7 days with oral nonabsorbable dosages of mezlocillin to achieve digestive tract decontamination. Such a procedure resulted in rapid eradication of most species of aerobic and anaerobic intestinal microflora. Various functions of peritoneal macrophages (e. g. chemiluminescence response, chemotactic motility, bactericidal and cytostatic ability) and lymphocyte proliferation were decreased in decontaminated animals as compared to non-treated controls.
BALB/c-Mäuse wurden sieben Tage oral mit Mezlocillin behandelt, um eine Elimination der aeroben und anaeroben Mikroflora des Gastrointestinaltraktes herbeizuführen und deren Auswirkungen zu studieren. Verglichen mit Kontrolltieren zeigten Mezlocillin-behandelte Mäuse mit dekontaminiertem Gastrointestinaltrakt eine Einschränkung der Phagozytose-Aktivität (Chemilumineszenz-Test), der chemotaktischen Beweglichkeit (Chemotaxis-Test), des direkten Abtötungseffektes (Bakterienabtötungstest) und des zytostatischen Effektes (Thymidin-Inkorporationstest) von Peritonealmakrophagen sowie Reduktion der Lymphozytenproliferation.
{"title":"Intestinal Microflora of BALB/c-Mice and Function of Local Immune Cells","authors":"K. Roszkowski , H.L. Ko , J. Beuth , Y. Ohshima , W. Roszkowski , J. Jeljaszewicz , G. Pulverer","doi":"10.1016/S0176-6724(88)80163-9","DOIUrl":"10.1016/S0176-6724(88)80163-9","url":null,"abstract":"<div><p>BALB/c-mice were treated for 7 days with oral nonabsorbable dosages of mezlocillin to achieve digestive tract decontamination. Such a procedure resulted in rapid eradication of most species of aerobic and anaerobic intestinal microflora. Various functions of peritoneal macrophages (e. g. chemiluminescence response, chemotactic motility, bactericidal and cytostatic ability) and lymphocyte proliferation were decreased in decontaminated animals as compared to non-treated controls.</p></div><div><p>BALB/c-Mäuse wurden sieben Tage oral mit Mezlocillin behandelt, um eine Elimination der aeroben und anaeroben Mikroflora des Gastrointestinaltraktes herbeizuführen und deren Auswirkungen zu studieren. Verglichen mit Kontrolltieren zeigten Mezlocillin-behandelte Mäuse mit dekontaminiertem Gastrointestinaltrakt eine Einschränkung der Phagozytose-Aktivität (Chemilumineszenz-Test), der chemotaktischen Beweglichkeit (Chemotaxis-Test), des direkten Abtötungseffektes (Bakterienabtötungstest) und des zytostatischen Effektes (Thymidin-Inkorporationstest) von Peritonealmakrophagen sowie Reduktion der Lymphozytenproliferation.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 270-279"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80163-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14350446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1988-11-01DOI: 10.1016/S0176-6724(88)80139-1
Werner Köhler
The CAMP test was proposed by Lesmana and Rockhill (11) as a method to differentiate between classical V. cholerae (negative) and V. cholerae biotype El Tor (positive). Typical CAMP phenomena with a crescent-like zone of complete hemolysis were observed also with some strains of V. metschnikovii, V. parahaemolyticus, and V. anguillarum when the cultures were incubated anaerobically (mostly also at aerobic conditions). No CAMP reaction was seen with V. harveyi and V. costicola.
{"title":"CAMP-like Phenomena of Vibrios","authors":"Werner Köhler","doi":"10.1016/S0176-6724(88)80139-1","DOIUrl":"10.1016/S0176-6724(88)80139-1","url":null,"abstract":"<div><p>The CAMP test was proposed by <em>Lesmana</em> and <em>Rockhill</em> (11) as a method to differentiate between classical V. cholerae (negative) and <em>V. cholerae</em> biotype <em>El Tor</em> (positive). Typical CAMP phenomena with a crescent-like zone of complete hemolysis were observed also with some strains of <em>V. metschnikovii, V. parahaemolyticus</em>, and <em>V. anguillarum</em> when the cultures were incubated anaerobically (mostly also at aerobic conditions). No CAMP reaction was seen with <em>V. harveyi</em> and <em>V. costicola</em>.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 35-40"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80139-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14350450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1988-11-01DOI: 10.1016/S0176-6724(88)80145-7
Mario Younan, Hans Schmid, Ernst Hellmann
135 Pasteurella strains were cultivated from nasal swabs of sheep as well as pneumonic lungs of dead and slaughtered sheep. The specimen originated from 41 flocks in South Germany and from 15 flocks and 60 slaughter sheep in Syria (Hama region).
Serovariety A2 prevailed amongst P. haemolytica strains (6) isolated in South Germany (53 strains) and in Syria (41 strains). In addition 10 further serovarieties were identified in South Germany (next frequent were A8, A1 and A6) and 7 in Syria. Untypable strains appeared to be more frequent in Syria.
Other Pasteurellae (17) represented ¼ of isolates in Syria and ⅓ of isolates in South Germany. Species identification resulted in P. multocida ssp. multocida (25), P. multocida ssp. septica (4 strains, Syria only), P. canis (3 strains, South Germany only) and Pasteurella-like strains (9 strains). Twelve P. multocida ssp. multocida strains carried capsular antigen D and 7 capsular antigen A.
In most cases where multiple samples were examined from one flock, strains with different capsular antigens and/or belonging to different Pasteurella species were isolated (max. 8).
{"title":"Species Identification and Serotyping (Capsular Antigen) of Pasteurella Strains from Sheep Flocks in South Germany and in Syria","authors":"Mario Younan, Hans Schmid, Ernst Hellmann","doi":"10.1016/S0176-6724(88)80145-7","DOIUrl":"10.1016/S0176-6724(88)80145-7","url":null,"abstract":"<div><p>135 <em>Pasteurella</em> strains were cultivated from nasal swabs of sheep as well as pneumonic lungs of dead and slaughtered sheep. The specimen originated from 41 flocks in South Germany and from 15 flocks and 60 slaughter sheep in Syria (Hama region).</p><p>Serovariety A2 prevailed amongst <em>P. haemolytica</em> strains (6) isolated in South Germany (53 strains) and in Syria (41 strains). In addition 10 further serovarieties were identified in South Germany (next frequent were A8, A1 and A6) and 7 in Syria. Untypable strains appeared to be more frequent in Syria.</p><p>Other <em>Pasteurellae</em> (17) represented ¼ of isolates in Syria and ⅓ of isolates in South Germany. Species identification resulted in <em>P. multocida ssp. multocida</em> (25), <em>P. multocida ssp. septica</em> (4 strains, Syria only), <em>P. canis</em> (3 strains, South Germany only) and <em>Pasteurella</em>-like strains (9 strains). Twelve <em>P. multocida ssp. multocida</em> strains carried capsular antigen D and 7 capsular antigen A.</p><p>In most cases where multiple samples were examined from one flock, strains with different capsular antigens and/or belonging to different <em>Pasteurella</em> species were isolated (max. 8).</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 98-109"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80145-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14350451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1988-11-01DOI: 10.1016/S0176-6724(88)80182-2
H.C. Korting , W. Maier , M. Schäfer-Korting
Two Staphylococcus aureus (S. aureus) strains with differing susceptibility to penicillin G as expressed by the minimum inhibitory concentration were exposed in vitro over a period of 8 h to the continuously changing concentrations of ofloxacin achieved in human serum and cantharides blister fluid (CBF) after the single oral application of 600 mg. In both strains bacterial density was rapidly, but not totally reduced: a reduction by 99 percent took no longer than about 1 and 1.5 h resp. facing the serum level profile and about 1.5 h facing the tissue level profile. The maximum reduction of staphylococcal density in percent (kn) amounted to 0.0007 and 0.0034 and to 0.0038 and 0.0047 resp. Thus not only the serum level profiles but also the skin tissue level profiles obtainable in man proved highly effective in vitro against S. aureus irrespective of the bacterial suspectibility against penicillin G. Therefore oral ofloxacin should prove useful in staphylococcal diseases especially in so far as cutaneous tissue is involved.
Zwei Staphylococcus aureus (S. aureus)-Stämme mit unterschiedlicher Penicillin G-Empfindlichkeit gemessen an der minimalen Hemmkonzentration (MHK) wurden in vitro über 8 h hinweg den ständig schwankenden Ofloxacin-Konzentrationen ausgesetzt, die beim Menschen in Serum- und Kantharidinblasenflüssigkeit (CBF) nach der einmaligen peroralen Gabe von 600 mg erreicht werden. Bei beiden Stämmen wurde die Bakteriendichte rasch, wenn auch nicht vollständig verringert: eine Reduktion um 99% beanspruchte in Gegenwart des Serumspiegelprofils nur etwa 1 bis 1,5 h.
In Gegenwart des Gewebsspiegelprofils lag dieser Wert bei etwa 1,5 h. Die maximale Verringerung der Staphylokokkendichte in Prozent (kn) belief sich auf 0,0007 und 0,0034, bzw. 0,0038 und 0,0047.
Somit erwies sich nicht nur das beim Menschen erreichbare Serumspiegelprofil, sondern auch das entsprechende Gewebsspiegelprofil als in vitro gegenüber S. aureus überaus wirksam. Dabei spielte die Penicillinempfindlichkeit der Erreger keine wesentliche Rolle. Die orale Anwendung von Ofloxacin bei Staphylokokkenerkrankungen dürfte sich demzufolge als nützlich erweisen, nicht zuletzt, was Hautinfektionen anbetrifft.
两株金黄色葡萄球菌(S. aureus)对青霉素G有不同的敏感性(以最小抑制浓度表示),在体外暴露于单次口服600mg氧氟沙星后,人血清和cantharides水泡液(CBF)中氧氟沙星浓度的连续变化中,时间为8小时。在这两种菌株中,细菌密度迅速下降,但不是完全下降:减少99%的时间分别不超过1和1.5小时。面向血清水平曲线,约1.5 h面向组织水平曲线。葡萄球菌密度最大降低百分比(kn)分别为0.0007和0.0034,0.0038和0.0047。因此,不仅血清水平谱,而且人体皮肤组织水平谱在体外证明对金黄色葡萄球菌非常有效,而不考虑细菌对青霉素g的怀疑。因此,口服氧氟沙星应该证明对葡萄球菌疾病有用,特别是在涉及皮肤组织的情况下。金黄色葡萄球菌(S. aureus)-Stämme体外培养青霉素G-Empfindlichkeit gemessen和der minimalen hemmkonconcentration (MHK) wurden, 8 h体外培养ständig schwankenden Ofloxacin-Konzentrationen ausgesetzt,血清中die beim Menschen -和kantharidinblasenflsigkeit (CBF) nach der inmaligen peroralen Gabe 600 mg erreicht werden。德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国德国0,0038和0,0047。一些研究表明,在体外培养金黄色葡萄球菌 (S. aureus berus)的研究中,有一些研究表明,在体外培养金黄色葡萄球菌 (S. aureus berus berirksam)。dabbei spielte die Penicillinempfindlichkeit der ergerine wesentliche Rolle。Die orale Anwendung von oflo沙星bei Staphylokokkenerkrankungen d rfte siich demzufolge als n tzlich erweisen, nicht zulezt,是Hautinfektionen anbetrifft。
{"title":"In vitro-bakterizidieeffekte von ofloxacin bei S. aureus","authors":"H.C. Korting , W. Maier , M. Schäfer-Korting","doi":"10.1016/S0176-6724(88)80182-2","DOIUrl":"https://doi.org/10.1016/S0176-6724(88)80182-2","url":null,"abstract":"<div><p>Two <em>Staphylococcus aureus (S. aureus)</em> strains with differing susceptibility to penicillin G as expressed by the minimum inhibitory concentration were exposed in vitro over a period of 8 h to the continuously changing concentrations of ofloxacin achieved in human serum and cantharides blister fluid (CBF) after the single oral application of 600 mg. In both strains bacterial density was rapidly, but not totally reduced: a reduction by 99 percent took no longer than about 1 and 1.5 h resp. facing the serum level profile and about 1.5 h facing the tissue level profile. The maximum reduction of staphylococcal density in percent (k<sub>n</sub>) amounted to 0.0007 and 0.0034 and to 0.0038 and 0.0047 resp. Thus not only the serum level profiles but also the skin tissue level profiles obtainable in man proved highly effective in vitro against <em>S. aureus</em> irrespective of the bacterial suspectibility against penicillin G. Therefore oral ofloxacin should prove useful in staphylococcal diseases especially in so far as cutaneous tissue is involved.</p></div><div><p>Zwei <em>Staphylococcus aureus (S. aureus)</em>-Stämme mit unterschiedlicher Penicillin G-Empfindlichkeit gemessen an der minimalen Hemmkonzentration (MHK) wurden in vitro über 8 h hinweg den ständig schwankenden Ofloxacin-Konzentrationen ausgesetzt, die beim Menschen in Serum- und Kantharidinblasenflüssigkeit (CBF) nach der einmaligen peroralen Gabe von 600 mg erreicht werden. Bei beiden Stämmen wurde die Bakteriendichte rasch, wenn auch nicht vollständig verringert: eine Reduktion um 99% beanspruchte in Gegenwart des Serumspiegelprofils nur etwa 1 bis 1,5 h.</p><p>In Gegenwart des Gewebsspiegelprofils lag dieser Wert bei etwa 1,5 h. Die maximale Verringerung der Staphylokokkendichte in Prozent (k<sub>n</sub>) belief sich auf 0,0007 und 0,0034, bzw. 0,0038 und 0,0047.</p><p>Somit erwies sich nicht nur das beim Menschen erreichbare Serumspiegelprofil, sondern auch das entsprechende Gewebsspiegelprofil als in vitro gegenüber <em>S. aureus</em> überaus wirksam. Dabei spielte die Penicillinempfindlichkeit der Erreger keine wesentliche Rolle. Die orale Anwendung von Ofloxacin bei Staphylokokkenerkrankungen dürfte sich demzufolge als nützlich erweisen, nicht zuletzt, was Hautinfektionen anbetrifft.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"269 3","pages":"Pages 387-394"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80182-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91983099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1988-11-01DOI: 10.1016/S0176-6724(88)80142-1
N.A. Saleh , A. Zwiefak , W. Peczyńska-Czoch , M. Mordarski , G. Pulverer
Two hydroxymethyltropolones and two tropolone acetate derivatives were found to inhibit an aminoglycoside-adenylyltranferase in a gentamicin-resistant Escherichia coli strain. The inhibitory effect of tropolones depends on the nature of the aminoglycoside antibiotic subject to adenylation.
Combinations of hydroxymethyltropolones with tobramycin were more active compared with tropolone acetates against gentamicin-resistant strains displaying adenylyltransferase activity. On the contrary a combination of the investigated acetate with gentamicin was of lower activity.
It could be shown that these inhibitors inhibit, to a varying degree, the transfer of radioactive ATP to different aminoglycoside molecules.
Zwei Hydroxymethyltropolone und zwei Tropolonacetat-Verbindungen hemmten die Aminoglykosid-Adenylyl-Transferase eines Gentamycin-resistenten Escherichia coli Stammes. Dieser Hemmeffekt der Tropolone hängt auch ab vom Aminoglykosid-Antibiotikum. Die Kombinationen von Hydroxymethyltropolonen mit Tobramycin waren aktiver als Tropolonazetate gegen Gentamycin-resistente Stämme mit Adenylyltransferase-Aktivität. Demgegenüber erwies sich die Kombination der untersuchten Azetate mit Gentamycin als schwächer wirksam. Es konnte gezeigt Verden, daß diese Hemmsubstanzen den Transfer der radioaktiven ATP auf verschiedene Aminoglykosid-Moleküle im wechselnden Ausmaß hemmten.
{"title":"New Inhibitors for Aminoglycoside-Adenylyltransferase","authors":"N.A. Saleh , A. Zwiefak , W. Peczyńska-Czoch , M. Mordarski , G. Pulverer","doi":"10.1016/S0176-6724(88)80142-1","DOIUrl":"10.1016/S0176-6724(88)80142-1","url":null,"abstract":"<div><p>Two hydroxymethyltropolones and two tropolone acetate derivatives were found to inhibit an aminoglycoside-adenylyltranferase in a gentamicin-resistant <em>Escherichia coli</em> strain. The inhibitory effect of tropolones depends on the nature of the aminoglycoside antibiotic subject to adenylation.</p><p>Combinations of hydroxymethyltropolones with tobramycin were more active compared with tropolone acetates against gentamicin-resistant strains displaying adenylyltransferase activity. On the contrary a combination of the investigated acetate with gentamicin was of lower activity.</p><p>It could be shown that these inhibitors inhibit, to a varying degree, the transfer of radioactive ATP to different aminoglycoside molecules.</p></div><div><p>Zwei Hydroxymethyltropolone und zwei Tropolonacetat-Verbindungen hemmten die Aminoglykosid-Adenylyl-Transferase eines Gentamycin-resistenten <em>Escherichia coli</em> Stammes. Dieser Hemmeffekt der Tropolone hängt auch ab vom Aminoglykosid-Antibiotikum. Die Kombinationen von Hydroxymethyltropolonen mit Tobramycin waren aktiver als Tropolonazetate gegen Gentamycin-resistente Stämme mit Adenylyltransferase-Aktivität. Demgegenüber erwies sich die Kombination der untersuchten Azetate mit Gentamycin als schwächer wirksam. Es konnte gezeigt Verden, daß diese Hemmsubstanzen den Transfer der radioaktiven ATP auf verschiedene Aminoglykosid-Moleküle im wechselnden Ausmaß hemmten.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 66-75"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80142-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13989390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1988-11-01DOI: 10.1016/S0176-6724(88)80172-X
D. Bruce , J.V. Hookey , Sheena A. Waitkins
Reference strains from five different species of Campylobacter were examined by DNA-restriction endonuclease analysis. The DNA profiles were resolved as absorbence peaks and converted to numerical values which were analysed by a Sirius microprocessor. The percentage similarity between each profile was calculated and the strains clustered together by the computer on shared similarities and the results displayed as an abbreviated dendrogram.
Each of the species of Campylobacter were clearly separated from each other and the different biotypes within a species could also be differentiated.
{"title":"Numerical classification of campylobacters by DNA-restriction endonuclease analysis","authors":"D. Bruce , J.V. Hookey , Sheena A. Waitkins","doi":"10.1016/S0176-6724(88)80172-X","DOIUrl":"10.1016/S0176-6724(88)80172-X","url":null,"abstract":"<div><p>Reference strains from five different species of <em>Campylobacter</em> were examined by DNA-restriction endonuclease analysis. The DNA profiles were resolved as absorbence peaks and converted to numerical values which were analysed by a Sirius microprocessor. The percentage similarity between each profile was calculated and the strains clustered together by the computer on shared similarities and the results displayed as an abbreviated dendrogram.</p><p>Each of the species of <em>Campylobacter</em> were clearly separated from each other and the different biotypes within a species could also be differentiated.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"269 3","pages":"Pages 284-297"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80172-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14197164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1988-11-01DOI: 10.1016/S0176-6724(88)80141-X
Britta S. Kubens, Wolfgang Opferkuch
Serum-sensitive mutants and their serum-resistant smooth parental E. coli strains (Wf8, Wf26, and WF 52) have been investigated in respect to their binding of different complement components. These pairs consisting of a wild-type and its mutants represent a better model for the investigation of the mechanism of serum resistance than the comparison of unrelated strains. Both strains of a pair bind equivalent amounts of C3. In binding assays using radiolabeled terminal components C6, C7, C8, and C9, the serum-sensitive strains do bind more late acting components than their resistant parental strains. An active membrane attack complex stably bound to the cell surface was found on the mutants, whereas with wild-type bacteria a complex could be isolated from the supernatant which is composed of the late acting complement components and S-protein. This complex is released from the surface of the wild-type bacteria without participation of C9.
{"title":"Studies on Serum Resistance in E scherichia coli","authors":"Britta S. Kubens, Wolfgang Opferkuch","doi":"10.1016/S0176-6724(88)80141-X","DOIUrl":"10.1016/S0176-6724(88)80141-X","url":null,"abstract":"<div><p>Serum-sensitive mutants and their serum-resistant smooth parental <em>E. coli</em> strains (Wf8, Wf26, and WF 52) have been investigated in respect to their binding of different complement components. These pairs consisting of a wild-type and its mutants represent a better model for the investigation of the mechanism of serum resistance than the comparison of unrelated strains. Both strains of a pair bind equivalent amounts of C3. In binding assays using radiolabeled terminal components C6, C7, C8, and C9, the serum-sensitive strains do bind more late acting components than their resistant parental strains. An active membrane attack complex stably bound to the cell surface was found on the mutants, whereas with wild-type bacteria a complex could be isolated from the supernatant which is composed of the late acting complement components and S-protein. This complex is released from the surface of the wild-type bacteria without participation of C9.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 52-65"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80141-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14277632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1988-11-01DOI: 10.1016/S0176-6724(88)80159-7
G. Pulverer , H.L. Ko , J. Beuth , K. Roszkowski , W. Roszkowski , J. Jeljaszewicz
Propionibacterium avidum KP 40 cells were mechanically disintegrated in order to obtain the soluble immunostimulatory (antineoplastic) LYSAT. Chemiluminescence measurements of human leukocyte function yielded enhanced activation of the cells after incubation with 2.5 and 5 mg of LYSAT. As compared to non-treated controls, administration of LYSAT to BALB/c-mice (1, 2.5 and 5 mg; intraperitoneally, subcutaneously, intranasal; 7, 4 and 2 days prior to challenge) induced a significant enlargement of the spleen as well as significantly reduced sarcoma L-1 lung colonization 14 days after challenge and evidently enhanced chemiluminescence response of peritoneal macrophages.
Propionibacterium avidum KP 40 Zellen wurden in einer Zellmühle mechanisch aufgelöst und weiter verarbeitet zum Immunstimulans LYSAT. Im Chemilumineszenz-Test offenbarten menschliche Leukozyten (Granulozyten und Monozyten) nach Inkubation mit 2,5 und 5 mg LYSAT eine gesteigerte Aktivität. Im Vergleich mit einer unbehandelten Kontroll-gruppe bewirkte die dreimalige LYSAT-Gabe in BALB/c-Mäusen (1, 2,5 und 5 mg; intraperitoneal, subkutan, nasal; 7, 4 und 2 Tage vor Testbeginn) eine signifikante Gewichtszunahme der Milz sowie eine signifikant reduzierte Anzahl von Lungenmetastasen 14 Tage nach Tumorzellinokulation und eine deutlich gesteigerte Aktivität der Peritoneal-makrophagen im Chemilumineszenz-Assay.
采用机械崩解方法,获得可溶性免疫刺激(抗肿瘤)LYSAT。人白细胞功能的化学发光测量显示,在2.5和5mg LYSAT孵育后,细胞的活化增强。与未处理的对照组相比,给BALB/c小鼠LYSAT(1,2.5和5mg;腹腔内、皮下、鼻内;攻毒前7、4和2天)诱导脾脏显著增大,攻毒后14天显著减少肉瘤L-1肺定植,并明显增强腹腔巨噬细胞的化学发光反应。猪丙酸杆菌KP 40 Zellen wurden在einer zellm机制aufgelöst和weiter verarbeitet zum免疫刺激剂LYSAT。每次孵育2、5、5 mg LYSAT eine gesteigerte Aktivität。在BALB/c-Mäusen(1、2、5和5 mg)中对LYSAT-Gabe进行预处理的对照组;腹膜内,腹下,鼻;7 4 2天伏尔Testbeginn)一张signifikante Gewichtszunahme der Milz和一张signifikant reduzierte Anzahl冯Lungenmetastasen 14天Tumorzellinokulation票和一张deutlich gesteigerte Aktivitat der Peritoneal-makrophagen Chemilumineszenz-Assay化生。
{"title":"Studies on a Biological Response Modifying LYSAT from Propionibacterium avidum KP 40","authors":"G. Pulverer , H.L. Ko , J. Beuth , K. Roszkowski , W. Roszkowski , J. Jeljaszewicz","doi":"10.1016/S0176-6724(88)80159-7","DOIUrl":"10.1016/S0176-6724(88)80159-7","url":null,"abstract":"<div><p><em>Propionibacterium avidum</em> KP 40 cells were mechanically disintegrated in order to obtain the soluble immunostimulatory (antineoplastic) LYSAT. Chemiluminescence measurements of human leukocyte function yielded enhanced activation of the cells after incubation with 2.5 and 5 mg of LYSAT. As compared to non-treated controls, administration of LYSAT to BALB/c-mice (1, 2.5 and 5 mg; intraperitoneally, subcutaneously, intranasal; 7, 4 and 2 days prior to challenge) induced a significant enlargement of the spleen as well as significantly reduced sarcoma L-1 lung colonization 14 days after challenge and evidently enhanced chemiluminescence response of peritoneal macrophages.</p></div><div><p><em>Propionibacterium avidum</em> KP 40 Zellen wurden in einer Zellmühle mechanisch aufgelöst und weiter verarbeitet zum Immunstimulans LYSAT. Im Chemilumineszenz-Test offenbarten menschliche Leukozyten (Granulozyten und Monozyten) nach Inkubation mit 2,5 und 5 mg LYSAT eine gesteigerte Aktivität. Im Vergleich mit einer unbehandelten Kontroll-gruppe bewirkte die dreimalige LYSAT-Gabe in BALB/c-Mäusen (1, 2,5 und 5 mg; intraperitoneal, subkutan, nasal; 7, 4 und 2 Tage vor Testbeginn) eine signifikante Gewichtszunahme der Milz sowie eine signifikant reduzierte Anzahl von Lungenmetastasen 14 Tage nach Tumorzellinokulation und eine deutlich gesteigerte Aktivität der Peritoneal-makrophagen im Chemilumineszenz-Assay.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 237-245"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80159-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14350445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1988-11-01DOI: 10.1016/S0176-6724(88)80148-2
W. Mathys , E. Junge , Ch. Sobek-Pfeiffer , H. Bösenberg , M. von Eiff
For the outbreak of diseases caused by Legionellae an infection by contaminated water is thought to be responsible. In the environment of patients with a high risk of infection (e. g. after kidney transplantation, immunosuppression) prophylactic measures (rising the temperature of the warm water to 60 °C) should be combined with bacteriological controls. It has to be taken into account, however, that only by taking a couple of samples from the same outlet during a period of time a colonization of central installation systems (sediments, storage tanks) can be discovered. In patients with acute pneumonia of unknown origin serological tests of Legionella antibodies are not very specific. Antibody titers of not infected and with Legionella infected patients show no significant difference. Well suited for the diagnosis of a legionellosis is the use of the Direct Immunofluorescent Antibody Assay (DFA) in invasively sampled specimens. In immunocompromised pneumonia patients 18.3% of bronchoalveolar lavages and 16.0% of bronchoalveolar aspirates are Legionella positive.
Für den Ausbruch von Legionellen-Erkrankungen wird im allgemeinen die Infektion durch kontaminiertes Wasser als Ursache angenommen. In der Umgebung gefährdeter Patienten (z.B. nach Nierentransplantation, Immunsuppression) sollten deshalb vorsorgliche Maßnahmen (Erhöhung der Warmwassertemperatur auf 60 °C) mit bakteriologischen Kontrollen kombiniert werden. Dabei ist zu bedenken, daß nur durch Erfassen mehrerer Wasserproben nach dem öffnen der Wasserzapfstelle zentrale Keimreservoire (z. B. Sediment, Wassertanks) erkennbar werden. Bei akuten unklaren Lungenerkrankungen sind serologische Untersuchungen auf Legionellenantikörper wenig spezifisch. Antikörpertiter von nicht-infizierten und mit Legionella infizierten Patienten zeigen keine signifikanten Unterschiede. Als gut geeignete Methode zur Diagnose einer Legionellose erweist sich der Einsatz des Direkten Immunofluoreszenztests (DFA) bei bronchoskopisch gewonnenen Materialien. Bei immunsupprimierten Pneumonie-Patienten ergaben sich 18,3% positive Befunde in der Bronchiallavage und 16,0% im Bronchialsekret.
对于军团菌引起的疾病爆发,被污染的水的感染被认为是负责任的。在感染高危患者的环境中(如肾移植后、免疫抑制),应采取预防措施(将温水温度升高至60℃)并结合细菌学控制。然而,必须考虑到,只有在一段时间内从同一出口采集几个样本,才能发现中央安装系统(沉积物,储罐)的定植。在不明原因急性肺炎患者中,军团菌抗体的血清学检测不具有特异性。未感染和军团菌感染患者的抗体滴度无显著差异。在侵入性取样标本中使用直接免疫荧光抗体测定(DFA)非常适合军团菌病的诊断。在免疫功能低下的肺炎患者中,18.3%的支气管肺泡灌洗液和16.0%的支气管肺泡吸出液呈军团菌阳性。[8] [j] [j] [j] [j] [j] [j]。In der Umgebung gefährdeter Patienten (z.b.n ach Nierentransplantation, immunosuppression) sollten deshalb vorsorgliche Maßnahmen (Erhöhung der warmwassertemperature auf 60°C) mit bakteriologischen Kontrollen kombiniert werden。Dabei ist zu bedenken, dasunur durch Erfassen mehrerer Wasserproben nach dem öffnen der Wasserzapfstelle zentrale keimreservoir (z. B. Sediment, Wassertanks) erkennbar werden。北碚akuten unklaren Lungenerkrankungen sinologische Untersuchungen auf Legionellenantikörper wenig spezifisch。Antikörpertiter von nicht-infizierten and mit Legionella infizierten patientzeigen keine signalkanten Unterschiede。采用直接免疫荧光试验(DFA)诊断支气管肺组织病变的方法。免疫原性肺炎患者中,支气管灌洗液中Befunde阳性18.3%,支气管灌洗液中Befunde阳性16.0%。
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Pub Date : 1988-11-01DOI: 10.1016/S0176-6724(88)80158-5
Rea Krausse , Uwe Ullmann , Christel Wagener
The influence of Mycoplasma species ( sp.) on the stimulation of human polymorphonuclear neutrophil granulocytes (PMNG) was determined by means of the luminol-dependent chemiluminescence (CL) method. When opsonized Mycoplasma sp. were used the CL response of PMNG was greater than in the presence of nonopsonized strains. Nonopsonized and nonspecifically opsonized Mycoplasma sp. showed a different CL response pattern. The stimulation of PMNG was with M. pneumoniae significantly weaker than with the other Mycoplasma sp. Using isolated M. hominis strains always the same CL-reaction of PMNG was observed. On the other hand, with 12 isolated U. urealyticum strains different results were obtained; 9 strains isolated from the upper urogenital tract lead to a slight PMNG stimulation comparable to that of M. pneumoniae. No correlation was found between CL response and bacterial killing. The weak stimulation of PMNG by M. pneumoniae and most of the U. urealyticum isolates suggest that this behaviour could be a factor of pathogenicity.
{"title":"In Vitro Influence of Mycoplasma Species on the Stimulation of Human Polymorphonuclear Granulocytes","authors":"Rea Krausse , Uwe Ullmann , Christel Wagener","doi":"10.1016/S0176-6724(88)80158-5","DOIUrl":"10.1016/S0176-6724(88)80158-5","url":null,"abstract":"<div><p>The influence of <em>Mycoplasma</em> species ( sp.) on the stimulation of human polymorphonuclear neutrophil granulocytes (PMNG) was determined by means of the luminol-dependent chemiluminescence (CL) method. When opsonized <em>Mycoplasma</em> sp. were used the CL response of PMNG was greater than in the presence of nonopsonized strains. Nonopsonized and nonspecifically opsonized <em>Mycoplasma</em> sp. showed a different CL response pattern. The stimulation of PMNG was with <em>M. pneumoniae</em> significantly weaker than with the other <em>Mycoplasma</em> sp. Using isolated <em>M. hominis</em> strains always the same CL-reaction of PMNG was observed. On the other hand, with 12 isolated <em>U. urealyticum</em> strains different results were obtained; 9 strains isolated from the upper urogenital tract lead to a slight PMNG stimulation comparable to that of <em>M. pneumoniae</em>. No correlation was found between CL response and bacterial killing. The weak stimulation of PMNG by <em>M. pneumoniae</em> and most of the <em>U. urealyticum</em> isolates suggest that this behaviour could be a factor of pathogenicity.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 228-236"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80158-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14277630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}