Pub Date : 2024-05-28DOI: 10.2174/0115734110290125240520062925
Wenjun Jiang, Xiaodi Guo, Shanbo Ma, Yuanhong Chang, Ya Li
Background: Gastric cancer is a prevalent malignant tumor globally, with metastasis and recurrence serving as primary contributors to the diminished survival rates observed in patients with advanced stages of the disease. Despite numerous investigations into the pathogenesis of gastric cancer, the significance of m6A demethylase ALKBH5 in the initiation and progression of the disease has been established, yet its precise mechanism remains incompletely understood. Methods: Immunohistochemistry was utilized for the evaluation of ALKBH5 expression in gastric cancer tissues, while RT-qPCR and WB were employed for the analysis of ALKBH5 expression in cells. Additionally, the levels of m6A methylation in RNA were assessed using the EpiQuikTM m6A methylation quantitative assay kit. The proliferative and metastatic potentials of gastric cancer cells were assessed using the CCK-8 and Transwell cell assays, respectively. The binding site between miR-625-5p and ALKBH5 was predicted through the TargetScan website, and the interaction was confirmed using a dual luciferase reporter assay. Results: The research findings indicated a significant increase in m6A methylation levels in gastric cancer cells, concomitant with a substantial decrease in ALKBH5 expression. ALKBH5 can inhibit the proliferation and metastasis of gastric cancer cells. Additionally, the interaction between miR- 625-5p and the 3'-UTR of ALKBH5 mRNA, resulting in the suppression of ALKBH5 expression, was validated. Conclusions: Through its specific interaction with ALKBH5, miR-625-5p modulates the proliferation and metastatic potential of gastric cancer cells, bridging a previously unexplored link in the pathogenesis of gastric cancer between miR-625-5p and ALKBH5. This finding suggests that miR- 625-5p could be a potential target for treating gastric cancer, with the goal of enhancing patient outcomes and quality of life.
{"title":"MiR-625-5p Modulates the Metastasis and Proliferation of Gastric Cancer Cells by Governing the M6A Demethylase ALKBH5","authors":"Wenjun Jiang, Xiaodi Guo, Shanbo Ma, Yuanhong Chang, Ya Li","doi":"10.2174/0115734110290125240520062925","DOIUrl":"https://doi.org/10.2174/0115734110290125240520062925","url":null,"abstract":"Background: Gastric cancer is a prevalent malignant tumor globally, with metastasis and recurrence serving as primary contributors to the diminished survival rates observed in patients with advanced stages of the disease. Despite numerous investigations into the pathogenesis of gastric cancer, the significance of m6A demethylase ALKBH5 in the initiation and progression of the disease has been established, yet its precise mechanism remains incompletely understood. Methods: Immunohistochemistry was utilized for the evaluation of ALKBH5 expression in gastric cancer tissues, while RT-qPCR and WB were employed for the analysis of ALKBH5 expression in cells. Additionally, the levels of m6A methylation in RNA were assessed using the EpiQuikTM m6A methylation quantitative assay kit. The proliferative and metastatic potentials of gastric cancer cells were assessed using the CCK-8 and Transwell cell assays, respectively. The binding site between miR-625-5p and ALKBH5 was predicted through the TargetScan website, and the interaction was confirmed using a dual luciferase reporter assay. Results: The research findings indicated a significant increase in m6A methylation levels in gastric cancer cells, concomitant with a substantial decrease in ALKBH5 expression. ALKBH5 can inhibit the proliferation and metastasis of gastric cancer cells. Additionally, the interaction between miR- 625-5p and the 3'-UTR of ALKBH5 mRNA, resulting in the suppression of ALKBH5 expression, was validated. Conclusions: Through its specific interaction with ALKBH5, miR-625-5p modulates the proliferation and metastatic potential of gastric cancer cells, bridging a previously unexplored link in the pathogenesis of gastric cancer between miR-625-5p and ALKBH5. This finding suggests that miR- 625-5p could be a potential target for treating gastric cancer, with the goal of enhancing patient outcomes and quality of life.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"55 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141167627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-13DOI: 10.2174/0115734110304194240503103902
Xiaoting Wang, Yingdong Yang, Tianyou Wang, Wugan Luo
Background: Bronze knives, which have been excavated in large quantities and acquired hierarchical significance, are essential artifacts in Shu State in Southwest China. Building upon previous typological analyses of bronze knives, it is hypothesized that Shu culture may have imported foreign-style bronze knives. However, further demonstration of the provenance of metal materials, the typology of knives, and the cultural exchange necessitates a comprehensive examination through the lens of scientific analysis. The purpose of this study is to investigate the differences in the manufacturing processes and metal resources of Shu bronze knives with various cultural styles and whether bronze knives in the foreign styles were imported or locally imitated. Methods: In this study, the typology, portable X-ray fluorescence spectrometry and multi-collector inductively coupled plasma mass spectrometry were used to analyze twenty-four bronze knives unearthed from Shuangyuan cemetery, a cemetery of the Eastern Zhou Dynasty in Chengdu City, Sichuan Province, Southwest China. Results: The results of the study show that the knives of Shuangyuan Cemetery can be classified typologically into five types, encompassing both local and foreign styles. The predominant alloy composition of these knives is lead-tin bronze, characterized by a notably high tin content. Lead isotope ratios indicate that metal materials from the South China geochemical province and Yangtze geochemical province were mainly used to manufacture the bronze knives unearthed from the Shu state Conclusion: While the majority of foreign-style knives with ring-shaped heads were likely imported from neighboring regions like Chu state, distinctively styled type D and type E bronze knives appear to have been locally imitated by the Shu, showcasing a blend of external influences and indigenous innovation. The combination of typology and scientific analysis of the bronze knife may shed new light on the study of the Shu culture of the Eastern Zhou period.
背景：青铜刀是中国西南地区蜀国的重要器物，出土量大，具有等级意义。根据以往对青铜刀的类型学分析，推测蜀文化可能曾传入过外来风格的青铜刀。然而，要进一步证明金属材料的来源、刀的类型和文化交流，就必须通过科学分析的视角进行全面考察。本研究的目的是探讨不同文化风格的蜀国青铜刀在制造工艺和金属资源方面的差异，以及外来风格的青铜刀是舶来品还是本地仿制品。研究方法：本研究采用类型学、便携式 X 射线荧光光谱仪和多收集器电感耦合等离子体质谱仪对四川省成都市双元东周墓地出土的 24 件青铜刀进行了分析。研究结果研究结果表明，双源墓地出土的青铜刀从类型上可分为五种，既有本地风格，也有外来风格。这些刀具的主要合金成分是铅锡青铜，其特点是锡含量明显偏高。铅同位素比值表明，蜀国墓地出土的青铜刀主要使用了华南地球化学省和长江地球化学省的金属材料：大部分外来的环首刀可能是从楚国等周边地区传入的，而风格独特的D型和E型青铜刀似乎是蜀人在当地仿制的，体现了外来影响与本土创新的融合。将青铜刀的类型学与科学分析相结合，可为东周时期的蜀文化研究提供新的启示。
{"title":"A Typology and Lead Isotope and Cultural Exchange Study on Bronze Knives from Shuangyuan Cemetery, Chengdu City, Southwest China","authors":"Xiaoting Wang, Yingdong Yang, Tianyou Wang, Wugan Luo","doi":"10.2174/0115734110304194240503103902","DOIUrl":"https://doi.org/10.2174/0115734110304194240503103902","url":null,"abstract":"Background: Bronze knives, which have been excavated in large quantities and acquired hierarchical significance, are essential artifacts in Shu State in Southwest China. Building upon previous typological analyses of bronze knives, it is hypothesized that Shu culture may have imported foreign-style bronze knives. However, further demonstration of the provenance of metal materials, the typology of knives, and the cultural exchange necessitates a comprehensive examination through the lens of scientific analysis. The purpose of this study is to investigate the differences in the manufacturing processes and metal resources of Shu bronze knives with various cultural styles and whether bronze knives in the foreign styles were imported or locally imitated. Methods: In this study, the typology, portable X-ray fluorescence spectrometry and multi-collector inductively coupled plasma mass spectrometry were used to analyze twenty-four bronze knives unearthed from Shuangyuan cemetery, a cemetery of the Eastern Zhou Dynasty in Chengdu City, Sichuan Province, Southwest China. Results: The results of the study show that the knives of Shuangyuan Cemetery can be classified typologically into five types, encompassing both local and foreign styles. The predominant alloy composition of these knives is lead-tin bronze, characterized by a notably high tin content. Lead isotope ratios indicate that metal materials from the South China geochemical province and Yangtze geochemical province were mainly used to manufacture the bronze knives unearthed from the Shu state Conclusion: While the majority of foreign-style knives with ring-shaped heads were likely imported from neighboring regions like Chu state, distinctively styled type D and type E bronze knives appear to have been locally imitated by the Shu, showcasing a blend of external influences and indigenous innovation. The combination of typology and scientific analysis of the bronze knife may shed new light on the study of the Shu culture of the Eastern Zhou period.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"137 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140940154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Corrosion of mild steel is a risk to material and stability. The practice of corrosion inhibitors is a cost-effective corrosion modification method for mild steel. Organic inhibitors rich in electrons might have an excellent ability to prevent corrosion. This study aims to assess the inhibitory effect of the mixture of Urea, Zinc Sulfate, and L-Phenylalanine, which has a high electron density Methods: MS corrosion was experimentally performed using H2SO4 at a pH of 4. Different gravimetric and conventional techniques, such as polarization, AC impedance AFM, UV, and fluorescence, were used to examine the studied data. Results: According to gravimetric measurements, this combination produced 93% effective inhibition. The findings of the impedance test proved that the mixture of inhibitors that was adsorbed on the metal surface effectively prevented corrosion. Conclusion: Likewise, according to the Polarization measurements, the inhibitor exhibits mixed-type performance with significant cathodic activity. UV, Fluorescence, and AFM findings showed that MS corrosion was suppressed because the inhibitor molecule adhered to the metal's surface and reduced.
{"title":"Investigating the Effects of Urea-Zinc Sulfate-L Phenylalanine on the Corrosion Inhibition of Mild Steel Exposed to pH-4 Sulfuric Acid","authors":"M.B Geetha, Rajesh Nithyanandam, Jenish Soosai, S.S. Rajendran","doi":"10.2174/0115734110296231240501170801","DOIUrl":"https://doi.org/10.2174/0115734110296231240501170801","url":null,"abstract":"Background: Corrosion of mild steel is a risk to material and stability. The practice of corrosion inhibitors is a cost-effective corrosion modification method for mild steel. Organic inhibitors rich in electrons might have an excellent ability to prevent corrosion. This study aims to assess the inhibitory effect of the mixture of Urea, Zinc Sulfate, and L-Phenylalanine, which has a high electron density Methods: MS corrosion was experimentally performed using H2SO4 at a pH of 4. Different gravimetric and conventional techniques, such as polarization, AC impedance AFM, UV, and fluorescence, were used to examine the studied data. Results: According to gravimetric measurements, this combination produced 93% effective inhibition. The findings of the impedance test proved that the mixture of inhibitors that was adsorbed on the metal surface effectively prevented corrosion. Conclusion: Likewise, according to the Polarization measurements, the inhibitor exhibits mixed-type performance with significant cathodic activity. UV, Fluorescence, and AFM findings showed that MS corrosion was suppressed because the inhibitor molecule adhered to the metal's surface and reduced.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"25 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140940224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-13DOI: 10.2174/0115734110300593240325060533
Safa S. EL-Sanafery, Khaled M. Hussein, Ashraf A. Abbas, M. M. Omar, Gehad G. Mohamed
Background and Objective: This study presents a novel potentiometric method for the precise, accurate, selective, and rapid determination of Cr(III) ion concentration in different samples. Methods: A new ionophore, namely macrocyclic tetramide ionophore (MCTA), was synthesized through an inexpensive and straightforward approach, yielding a high-quality product. The (MCTA) ionophore was utilized as the active center in the preparation of modified carbon paste electrodes (MCPEs) to quantify the Cr(III) ion. The paste was made by adding graphite, MCTA, and plasticizer and mixing them in varying weight percent ratios. Results: The proposed electrodes, I and II, exhibited a trivalent Nernstian response of 20.029 ±0.57 and 20.3±0.56 mV decade-1 , respectively, with linearity of 1.0x10-7 – 1.0x10-2 and 1.0x10-5 – 1.0x10- 2 mol L-1 . Electrodes I and II were examined for their pH, response time, and thermal stability. In comparison to other mono-, bi-, and trivalent cations, starch, and sugars, the electrodes demonstrated a high degree of selectivity for Cr(III). The modified electrodes were used to determine the concentration of Cr(III) in various real samples, including drug tablets, juice extractions, and tap water, with acceptable recovery values. Conclusion: The results were compared with those obtained using the previously reported method, with no significant difference observed between them, as indicated by the F and t-test values. The data showed good accuracy and precision, as well as a high percentage of recovery. The adsorption capacity of the MCTA ionophore towards Cr(III) ions was also examined.
背景与目的：本研究提出了一种新颖的电位法，用于精确、准确、选择性地快速测定不同样品中的 Cr(III) 离子浓度。方法：通过一种廉价而简单的方法合成了一种新的离子源，即大环四胺离子源（MCTA），并获得了高质量的产品。在制备改性碳浆电极（MCPEs）时，利用（MCTA）离子团作为活性中心来定量检测铬（III）离子。将石墨、MCTA 和增塑剂按不同的重量百分比比例混合制成碳浆。结果：拟制电极 I 和 II 的三价 Nernstian 响应分别为 20.029 ±0.57 和 20.3±0.56 mV decade-1，线性度分别为 1.0x10-7 - 1.0x10-2 和 1.0x10-5 - 1.0x10- 2 mol L-1。对电极 I 和 II 的 pH 值、响应时间和热稳定性进行了检测。与其他一价、二价和三价阳离子、淀粉和糖相比，电极对 Cr(III) 具有高度的选择性。改良电极可用于测定各种实际样品（包括药物片剂、果汁提取物和自来水）中的三价铬浓度，且回收率可接受。结论将结果与之前报告的方法进行了比较，从 F 检验值和 t 检验值可以看出，两者之间没有显著差异。数据显示了良好的准确度和精确度，以及较高的回收率。此外，还考察了 MCTA 离子团对 Cr(III) 离子的吸附能力。
{"title":"A New Modified Carbon Paste Electrode for Selective Determination of Chromium(III) in Pharmaceutical Drugs and Food Samples","authors":"Safa S. EL-Sanafery, Khaled M. Hussein, Ashraf A. Abbas, M. M. Omar, Gehad G. Mohamed","doi":"10.2174/0115734110300593240325060533","DOIUrl":"https://doi.org/10.2174/0115734110300593240325060533","url":null,"abstract":"Background and Objective: This study presents a novel potentiometric method for the precise, accurate, selective, and rapid determination of Cr(III) ion concentration in different samples. Methods: A new ionophore, namely macrocyclic tetramide ionophore (MCTA), was synthesized through an inexpensive and straightforward approach, yielding a high-quality product. The (MCTA) ionophore was utilized as the active center in the preparation of modified carbon paste electrodes (MCPEs) to quantify the Cr(III) ion. The paste was made by adding graphite, MCTA, and plasticizer and mixing them in varying weight percent ratios. Results: The proposed electrodes, I and II, exhibited a trivalent Nernstian response of 20.029 ±0.57 and 20.3±0.56 mV decade-1 , respectively, with linearity of 1.0x10-7 – 1.0x10-2 and 1.0x10-5 – 1.0x10- 2 mol L-1 . Electrodes I and II were examined for their pH, response time, and thermal stability. In comparison to other mono-, bi-, and trivalent cations, starch, and sugars, the electrodes demonstrated a high degree of selectivity for Cr(III). The modified electrodes were used to determine the concentration of Cr(III) in various real samples, including drug tablets, juice extractions, and tap water, with acceptable recovery values. Conclusion: The results were compared with those obtained using the previously reported method, with no significant difference observed between them, as indicated by the F and t-test values. The data showed good accuracy and precision, as well as a high percentage of recovery. The adsorption capacity of the MCTA ionophore towards Cr(III) ions was also examined.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"18 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140942466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-08DOI: 10.2174/0115734110303211240430161337
Yankai Liang, Kinjal J. Shah, Yongjun Sun
:: Nanoparticles are widely used in manufacturing, daily life applications, and other fields because of their unique properties. However, the nanoparticles that end up in surface water are difficult to break down naturally and cause environmental hazards that cannot be ignored. In this paper, the migration mode of nanoparticles in the environment was proposed, and the factors affecting the removal efficiency of nanoparticles mainly include ionic strength, natural organic matter, surface properties, etc. The common methods for removing nanoparticles mainly include coagulation precipitation methods, activated sludge methods, membrane filtration methods, and adsorption methods. In addition, new technologies such as photothermal removal and electro-adsorption have also been applied to remove nanoparticles in water. In future research, combining the advantages of different removal methods and using different methods interactively to remove nanoparticles in water could become a new research direction.
{"title":"Recent Advances in Technologies for Removing Nanoparticles from Water","authors":"Yankai Liang, Kinjal J. Shah, Yongjun Sun","doi":"10.2174/0115734110303211240430161337","DOIUrl":"https://doi.org/10.2174/0115734110303211240430161337","url":null,"abstract":":: Nanoparticles are widely used in manufacturing, daily life applications, and other fields because of their unique properties. However, the nanoparticles that end up in surface water are difficult to break down naturally and cause environmental hazards that cannot be ignored. In this paper, the migration mode of nanoparticles in the environment was proposed, and the factors affecting the removal efficiency of nanoparticles mainly include ionic strength, natural organic matter, surface properties, etc. The common methods for removing nanoparticles mainly include coagulation precipitation methods, activated sludge methods, membrane filtration methods, and adsorption methods. In addition, new technologies such as photothermal removal and electro-adsorption have also been applied to remove nanoparticles in water. In future research, combining the advantages of different removal methods and using different methods interactively to remove nanoparticles in water could become a new research direction.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"32 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140940157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
:: In recent years, research and development efforts have been heavily focused on conductive diamond electrodes for electrochemical applications. Such initiatives may have been spurred by their broad potential window, low background current, chemical inertness, and mechanical robustness. Compared to other carbon-based materials, conducting diamond can oxidize several analytes before the breakdown of water in aqueous electrolytes. Since the evolution of oxygen and hydrogen does not obstruct the analysis, this is significant for the detection and/or identification of species in solution. As a result, conductive diamond electrodes expand the application of electrochemical detection and make it possible to use them for analytes that are incompatible with traditional electrode materials. Fabricating boron-doped diamond films via chemical vapor deposition on different substrates is of special interest. This article highlights the therapeutic and electroanalytical applications of boron-doped diamond electrodes in various aspects in addition to the synthetic strategies to obtain Boron Doped Diamond Electrodes (BDDE), the cost-effectiveness of BDD and its in-vivo compatibility that will help the analytical researchers to learn almost everything about the previous studies done on BDDE and encourage them to work more efficiently in this research field.
{"title":"Recent Trends in Therapeutic and Electroanalytical Applications of Boron-Doped Diamond Sensors","authors":"Anish Soni, Prabhjot Kaur, Rohit Bhatia, Naresh Kumar Rangra","doi":"10.2174/0115734110304322240430053410","DOIUrl":"https://doi.org/10.2174/0115734110304322240430053410","url":null,"abstract":":: In recent years, research and development efforts have been heavily focused on conductive diamond electrodes for electrochemical applications. Such initiatives may have been spurred by their broad potential window, low background current, chemical inertness, and mechanical robustness. Compared to other carbon-based materials, conducting diamond can oxidize several analytes before the breakdown of water in aqueous electrolytes. Since the evolution of oxygen and hydrogen does not obstruct the analysis, this is significant for the detection and/or identification of species in solution. As a result, conductive diamond electrodes expand the application of electrochemical detection and make it possible to use them for analytes that are incompatible with traditional electrode materials. Fabricating boron-doped diamond films via chemical vapor deposition on different substrates is of special interest. This article highlights the therapeutic and electroanalytical applications of boron-doped diamond electrodes in various aspects in addition to the synthetic strategies to obtain Boron Doped Diamond Electrodes (BDDE), the cost-effectiveness of BDD and its in-vivo compatibility that will help the analytical researchers to learn almost everything about the previous studies done on BDDE and encourage them to work more efficiently in this research field.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"15 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140942503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-07DOI: 10.2174/0115734110294424240429040920
Linyang Wang, Aniwar Ikemu, Liwa Wang, Shuge Tian
Introduction: Leaves of Salvia deserta Schang at seven harvesting times in the same year were collected as the materials. Method: The polyphenols were determined by the Folin–Ciocaileu method and High-Performance Liquid Chromatography (HPLC) to compare the quality of samples. The stability of polyphenols was studied under different conditions (light, temperature, pH, common additives). objective: The contents of total polyphenols in samples of different growth stages were determined by Folin-Ciocaileu colorimetry. The contents of rosmarinic acid and caffeic acid in samples of different growth stages were determined by HPLC. The stability of polyphenols in Salvia deserta Schang Leaves was investigated by measuring the change of polyphenol retention rate in different media (light, temperature, pH, oxidant, sugar, preservative, salt and citric acid) Results: The results showed that the established method is fast, simple and reliable, which is fully validated in terms of outstanding validation data. High Performance Liquid Chromatography (HPLC) for the determination of total polyphenol content can be quickly and accurately detected, reducing the error of manual determination of the content. The study of polyphenol stability was carried out using a UV spectrophotometer (UV) in order to explore the potential factors affecting polyphenol stability as much as possible and to make the study as scientific and rigorous as possible. The results of quantitative determination showed that there are obvious differences in the content of polyphenols in seven samples. The contents of total polyphenols, rosmarinic acid (RA) and caffeic acid (CA) in the samples harvested in July reached the highest level of 41.37, 26.73 and 1.05 mg/g. Conclusion: The results of the stability assay found that light could damage the stability of polyphenols in samples, especially UV light. Polyphenols are quite sensitive to high temperatures. While polyphenols are less stable when exposed to high alkali conditions and salt treatment, they are much more stable when subjected to low concentrations of redox agents, carbohydrates, and preservatives. The developed methods and stability evaluation provide valuable basis information for quality evaluation and the following use of polyphenols in S. deserta Schang leaves.
{"title":"Studies on Quantitative Determination of Polyphenols in Seven Harvesting Times of Salvia deserta Schang Leaves and its Stability Evaluation","authors":"Linyang Wang, Aniwar Ikemu, Liwa Wang, Shuge Tian","doi":"10.2174/0115734110294424240429040920","DOIUrl":"https://doi.org/10.2174/0115734110294424240429040920","url":null,"abstract":"Introduction: Leaves of Salvia deserta Schang at seven harvesting times in the same year were collected as the materials. Method: The polyphenols were determined by the Folin–Ciocaileu method and High-Performance Liquid Chromatography (HPLC) to compare the quality of samples. The stability of polyphenols was studied under different conditions (light, temperature, pH, common additives). objective: The contents of total polyphenols in samples of different growth stages were determined by Folin-Ciocaileu colorimetry. The contents of rosmarinic acid and caffeic acid in samples of different growth stages were determined by HPLC. The stability of polyphenols in Salvia deserta Schang Leaves was investigated by measuring the change of polyphenol retention rate in different media (light, temperature, pH, oxidant, sugar, preservative, salt and citric acid) Results: The results showed that the established method is fast, simple and reliable, which is fully validated in terms of outstanding validation data. High Performance Liquid Chromatography (HPLC) for the determination of total polyphenol content can be quickly and accurately detected, reducing the error of manual determination of the content. The study of polyphenol stability was carried out using a UV spectrophotometer (UV) in order to explore the potential factors affecting polyphenol stability as much as possible and to make the study as scientific and rigorous as possible. The results of quantitative determination showed that there are obvious differences in the content of polyphenols in seven samples. The contents of total polyphenols, rosmarinic acid (RA) and caffeic acid (CA) in the samples harvested in July reached the highest level of 41.37, 26.73 and 1.05 mg/g. Conclusion: The results of the stability assay found that light could damage the stability of polyphenols in samples, especially UV light. Polyphenols are quite sensitive to high temperatures. While polyphenols are less stable when exposed to high alkali conditions and salt treatment, they are much more stable when subjected to low concentrations of redox agents, carbohydrates, and preservatives. The developed methods and stability evaluation provide valuable basis information for quality evaluation and the following use of polyphenols in S. deserta Schang leaves.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"120 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140940158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-07DOI: 10.2174/0115734110301044240426170020
Yangzhou Xie, Yi Yang, Yu Tian, Zhimin Liu, Zhigang Xu, Wei Jiang, Zhihua Liu, Xiaoxi Si
Background: Terpenoids are essential aroma substances in teas, and their concentration brings various characteristics to different teas. Therefore, developing a simple and stable method is necessary for distinguishing tea categories. Objective: In previous studies, more attention was paid to non-chiral isomers of terpenes due to the challenges of separating chiral isomers. So, this paper aims to present a method for effectively separating seven terpenoid substances, including chiral isomers and non-chiral isomers, to facilitate the classification and identification of teas. objective: In previous studies, more attention was paid to non-chiral isomers of terpeniods due to the challenges with separating chiral isomers. So, this paper aims to present a method for effectively separating seven terpenoid substances, including chiral isomers and non-chiral isomers, to facilitate the classification and identification of teas. Methods: A method utilizing headspace solid-phase microextraction coupled with gas chromatography- mass spectrometry was used to isolate and analyze 7 terpenoid compounds. After optimized conditions, the BGB-176 chiral column and the PDMS/DVB fiber were selected for subsequent analysis. Results: This method has a good linear range of 0.1-200 mg/L, and its linear correlation coefficients are between 0.9974 and 0.9994, and the limit of detection and the limit of quantification is 0.02–0.03 and 0.06–0.09 mg/L, respectively. Only five terpenoid substances were detected in a total of 15 tea samples. Furthermore, In the detection of carvon and α-ionone optical isomers, the S isomer was mainly detected. Conclusions: An effective approach was developed to separate and analyze 7 terpenoid compounds in natural and synthetic teas. Meanwhile, 15 tea samples can be identified and classified using principal component analysis.
{"title":"Analysis of Seven Terpenoids by HS-SPME Coupled with GC-MS for the Identification and Classification of Different Teas","authors":"Yangzhou Xie, Yi Yang, Yu Tian, Zhimin Liu, Zhigang Xu, Wei Jiang, Zhihua Liu, Xiaoxi Si","doi":"10.2174/0115734110301044240426170020","DOIUrl":"https://doi.org/10.2174/0115734110301044240426170020","url":null,"abstract":"Background: Terpenoids are essential aroma substances in teas, and their concentration brings various characteristics to different teas. Therefore, developing a simple and stable method is necessary for distinguishing tea categories. Objective: In previous studies, more attention was paid to non-chiral isomers of terpenes due to the challenges of separating chiral isomers. So, this paper aims to present a method for effectively separating seven terpenoid substances, including chiral isomers and non-chiral isomers, to facilitate the classification and identification of teas. objective: In previous studies, more attention was paid to non-chiral isomers of terpeniods due to the challenges with separating chiral isomers. So, this paper aims to present a method for effectively separating seven terpenoid substances, including chiral isomers and non-chiral isomers, to facilitate the classification and identification of teas. Methods: A method utilizing headspace solid-phase microextraction coupled with gas chromatography- mass spectrometry was used to isolate and analyze 7 terpenoid compounds. After optimized conditions, the BGB-176 chiral column and the PDMS/DVB fiber were selected for subsequent analysis. Results: This method has a good linear range of 0.1-200 mg/L, and its linear correlation coefficients are between 0.9974 and 0.9994, and the limit of detection and the limit of quantification is 0.02–0.03 and 0.06–0.09 mg/L, respectively. Only five terpenoid substances were detected in a total of 15 tea samples. Furthermore, In the detection of carvon and α-ionone optical isomers, the S isomer was mainly detected. Conclusions: An effective approach was developed to separate and analyze 7 terpenoid compounds in natural and synthetic teas. Meanwhile, 15 tea samples can be identified and classified using principal component analysis.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"44 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140940209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-07DOI: 10.2174/0115734110287027240427064546
Hanjing Ge
Background: Bacterial cellulose (BC) is a versatile biomaterial with numerous applications, and the identification of bacterial strains that produce it is of great importance. This study explores the effectiveness of a Stacked Autoencoder (SAE)-based deep learning method for the classification of bacterial cellulose-producing bacteria. Objective: The primary objective of this research is to assess the potential of SAE-based classification models in accurately identifying and classifying bacterial cellulose-producing bacteria, with a particular focus on strain GZ-01. objective: The primary objective of this research is to assess the potential of SAE-based classification models in accurately identifying and classifying bacterial cellulose-producing bacteria, with a particular focus on strain GZ-01. Methods: Strain GZ-01 was isolated and subjected to a comprehensive characterization process, including morphological observations, physiological and biochemical analysis, and 16S rDNA sequencing. These methods were employed to determine the identity of strain GZ-01, ultimately recognized as Acetobacter Okinawa. The study compares the performance of SAE-based classification models to traditional methods like Principal Component Analysis (PCA). Results: The SAE-based classifier exhibits outstanding performance, achieving an impressive accuracy of 94.9% in the recognition and classification of bacterial cellulose-producing bacteria. This approach surpasses the efficacy of conventional PCA in handling the complexities of this classification task. Conclusion: The findings from this research highlight the immense potential of utilizing nanotechnology- driven data analysis methods, such as Stacked Autoencoders, in the realm of bacterial cellulose research. These advanced techniques offer a promising avenue for enhancing the efficiency and accuracy of bacterial cellulose-producing bacteria classification, which has significant implications for various applications in biotechnology and materials science.
{"title":"Deep Learning-enhanced Hyperspectral Imaging for the Rapid Identification and Classification of Foodborne Pathogens","authors":"Hanjing Ge","doi":"10.2174/0115734110287027240427064546","DOIUrl":"https://doi.org/10.2174/0115734110287027240427064546","url":null,"abstract":"Background: Bacterial cellulose (BC) is a versatile biomaterial with numerous applications, and the identification of bacterial strains that produce it is of great importance. This study explores the effectiveness of a Stacked Autoencoder (SAE)-based deep learning method for the classification of bacterial cellulose-producing bacteria. Objective: The primary objective of this research is to assess the potential of SAE-based classification models in accurately identifying and classifying bacterial cellulose-producing bacteria, with a particular focus on strain GZ-01. objective: The primary objective of this research is to assess the potential of SAE-based classification models in accurately identifying and classifying bacterial cellulose-producing bacteria, with a particular focus on strain GZ-01. Methods: Strain GZ-01 was isolated and subjected to a comprehensive characterization process, including morphological observations, physiological and biochemical analysis, and 16S rDNA sequencing. These methods were employed to determine the identity of strain GZ-01, ultimately recognized as Acetobacter Okinawa. The study compares the performance of SAE-based classification models to traditional methods like Principal Component Analysis (PCA). Results: The SAE-based classifier exhibits outstanding performance, achieving an impressive accuracy of 94.9% in the recognition and classification of bacterial cellulose-producing bacteria. This approach surpasses the efficacy of conventional PCA in handling the complexities of this classification task. Conclusion: The findings from this research highlight the immense potential of utilizing nanotechnology- driven data analysis methods, such as Stacked Autoencoders, in the realm of bacterial cellulose research. These advanced techniques offer a promising avenue for enhancing the efficiency and accuracy of bacterial cellulose-producing bacteria classification, which has significant implications for various applications in biotechnology and materials science.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"46 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140940153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-03DOI: 10.2174/0115734110307066240425055358
Golchin Poryan, Maedeh Noori, Zahra Talebpour, Hassan Y. Aboul-Enein
Introduction: In this work, a polyacrylate polymer was synthesized into a pipette tip containing cotton fibers and used to extract carvedilol from water and urine samples. Methods: A high-performance liquid chromatography-ultraviolet detection method was developed, which demonstrated the suitability of the purposed pipette tip micro-solid-phase extraction device. Factors affecting the fabrication procedure and polymer quality were studied and optimized. In the next step, the sample preparation process (including extraction and desorption) was fully optimized, and the optimized method was validated. Results: A coating with suitable mechanical and chemical stability was achieved. Its structure was successfully characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. Within-batch and between-batch fabrication reproducibility were obtained at 3.0 and 9.0 %, respectively. The developed method displayed a limit of detection of 1.1 µg L-1 when 1.5 mL of sample was processed, and it was linear in the concentration range of 3.3-350 µg L-1 with LLOQ of 5 µg L-1 . The polyacrylate cotton-based pipette tip was finally used to extract carvedilol from aqueous media with acceptable recoveries of 92-106%. Conclusion: The proposed method is simple and fast and requires low sample volumes. In addition, this method has been evaluated in terms of greenness with three different tools, and the evaluation results with all three tools have shown that this method is one of the green and environmentally friendly methods.
{"title":"A Polyacrylate Cotton-based Pipette Tip Micro-solid-phase Extraction Technique Coupled with High-performance Liquid Chromatography for Carvedilol Determination in Aqueous Media","authors":"Golchin Poryan, Maedeh Noori, Zahra Talebpour, Hassan Y. Aboul-Enein","doi":"10.2174/0115734110307066240425055358","DOIUrl":"https://doi.org/10.2174/0115734110307066240425055358","url":null,"abstract":"Introduction: In this work, a polyacrylate polymer was synthesized into a pipette tip containing cotton fibers and used to extract carvedilol from water and urine samples. Methods: A high-performance liquid chromatography-ultraviolet detection method was developed, which demonstrated the suitability of the purposed pipette tip micro-solid-phase extraction device. Factors affecting the fabrication procedure and polymer quality were studied and optimized. In the next step, the sample preparation process (including extraction and desorption) was fully optimized, and the optimized method was validated. Results: A coating with suitable mechanical and chemical stability was achieved. Its structure was successfully characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. Within-batch and between-batch fabrication reproducibility were obtained at 3.0 and 9.0 %, respectively. The developed method displayed a limit of detection of 1.1 µg L-1 when 1.5 mL of sample was processed, and it was linear in the concentration range of 3.3-350 µg L-1 with LLOQ of 5 µg L-1 . The polyacrylate cotton-based pipette tip was finally used to extract carvedilol from aqueous media with acceptable recoveries of 92-106%. Conclusion: The proposed method is simple and fast and requires low sample volumes. In addition, this method has been evaluated in terms of greenness with three different tools, and the evaluation results with all three tools have shown that this method is one of the green and environmentally friendly methods.","PeriodicalId":10742,"journal":{"name":"Current Analytical Chemistry","volume":"95 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140834024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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