Critical reviews in analytical chemistry最新文献

A living cell is a complex network of molecular, biochemical and physiological processes. Cellular activities, such as ion transport, metabolic processes, and cell-cell interactions can be determined electrochemically by detecting the electrons or ions exchanged in these processes. Electrochemical methods often are noninvasive, and they can enable the real-time monitoring of cellular processes. Scanning electrochemical microscopy (SECM) is an advanced scanning probe electroanalysis technique that can map the surface topography and local reactivity of a substrate with high precision at the micro- or nanoscale. By measuring electrochemical signals, such as redox reactions, ion fluxes, and pH changes, SECM can provide valuable insights into cellular activity. As a result of its compatibility with liquid medium measurements and its nondestructive nature, SECM has gained popularity in living cell research. This review aims to furnish an overview of SECM, elucidating its principles, applications, and its potential to contribute significantly to advancements in cell biology, electroporation, and biosensors. As a multidisciplinary tool, SECM is distinguished by its ability to unravel the intricacies of living cells and offers promising avenues for breakthroughs in our understanding of cellular complexity.

Vitamin B₁₂ plays a significant role in maintaining human health. Deficiency or excess intake of vitamin B₁₂ may cause some diseases. Therefore, it is significant to fabricate sensors for sensitive assay of vitamin B₁₂. In the past few years, a variety of nanomaterials have been developed for the fluorescence detection of vitamin B₁₂ in tablets, injection, human serum and food. In the review, the assay mechanisms of fluorescent nanomaterials for sensing vitamin B₁₂ were first briefly discussed. And the progress of various nanomaterials for fluorescence detection of vitamin B₁₂ were systematically summarized. Furthermore, the sensing performance of fluorescent nanosensors was compared with fluorescent probes. Lastly, the challenges and perspectives about the topic were presented.

Drug resistance in microorganisms is a serious threat to life and health due to the limited number of antibiotics that show efficacy in treating infections and the difficulty in discovering new compounds with antibacterial activity. To address this issue, the World Health Organization created the AWaRe classification, a tool to support global and national antimicrobial stewardship programs. The AWaRe list categorizes antimicrobials into three groups - Access, Watch, and Reserve - according to their intended use. The Reserve group comprises "last resort" medicines used solely for treating infections caused by bacterial strains that are resistant to other treatments. It is therefore necessary to protect them, not only by using them as prudently as possible in humans and animals, but also by monitoring their subsequent fate. Unmetabolized antibiotics enter the environment through hospital and municipal wastewater or from manure, subsequently contaminating bodies of water and soils, thus contributing to the emergence and spread of antibiotic resistance. This article presents a review of determination methods for the Reserve group of antimicrobials in water, wastewater, and manure. Procedures for extracting and determining these substances in environmental samples are described, showing the limited research available, which is typically on a local level.

MicroRNA (miRNA) has emerged as a promising biomarker for disease diagnosis and a potential therapeutic targets for drug development. The detection of miRNA can serve as a noninvasive tool in diseases diagnosis and predicting diseases prognosis. CRISPR/Cas12a system has great potential in nucleic acid detection due to its high sensitivity and specificity, which has been developed to be a versatile tool for nucleic acid-based detection of targets in various fields. However, conversion from RNA to DNA with or without amplification operation is necessary for miRNA detection based on CRISPR/Cas12a system, because dsDNA containing PAM sequence or ssDNA is traditionally considered as the activator of Cas12a. Until recently, direct detection of miRNA by CRISPR/Cas12a system has been reported. In this review, we provide an overview of the evolution of biosensors based on CRISPR/Cas12a for miRNA detection from indirect to direct, which would be beneficial to the development of CRISPR/Cas12a-based sensors with better performance for direct detection of miRNA.