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EVALUATION OF CHLORELLA VULGARIS AS A SOURCE OF FERMENTABLE SUGAR USING AMYLASE PRODUCING BACILLUS CEREUS 利用产生淀粉酶的蜡样芽孢杆菌对普通小球藻作为发酵糖来源的评价
Pub Date : 2014-07-01 DOI: 10.5958/0976-4763.2014.00001.4
H. H. Migap, C. Whong, I. Abdullahi
Bacillus cereus that was previously isolated from the soil and screened for alpha-amylase production was used for hydrolysis of Chlorella vulgaris starch content. The Dinitrosalicylic acid method was used to determine the amount of reducing sugar produced. Maximum reducing sugar yield of 2.10 mg/ml was obtained for pre-treated C. vulgaris biomass, 2.06 mg/ml for untreated biomass and 2.56 mg/ml for corn starch (control) after 24 hours incubation. When the production parameters where optimized, maximum reducing sugar yield was obtained at a pH of 6.5, temperature of 35°C, incubation time of 24 hours and 4% inoculums concentration for pretreated C. vulgaris biomass. Also, a 1.3 fold reducing sugar yield was enhanced when compared with non-optimized conditions indicating the usefulness of optimization on biochemical processes. The maximum reducing sugar obtained for hydrolysis of pretreated C. vulgaris biomass is similar to the reducing sugar yield of corn starch. Also, the carbohydrate content of C. vulgaris hydrolysis was 66% which is comparable to the carbohydrate content of corn (about 73%). This indicates the potential of C. vulgaris as an alternative substrate for fermentable sugar production.
先前从土壤中分离并筛选产生α -淀粉酶的蜡样芽孢杆菌用于水解普通小球藻的淀粉含量。采用二硝基水杨酸法测定还原糖的产量。培养24小时后,预处理后的普通草生物量的最大还原糖产量为2.10 mg/ml,未处理的生物量为2.06 mg/ml,玉米淀粉(对照)的最大还原糖产量为2.56 mg/ml。优化生产参数后,在pH为6.5、温度为35℃、孵育时间为24 h、接种量为4%的条件下,预处理后的草菇生物量还原糖产量最大。此外,与未优化条件相比,还原糖产量提高了1.3倍,表明优化对生化过程的有效性。预处理后的木霉生物质水解得到的最大还原糖量与玉米淀粉的还原糖量相近。同时,木豆水解后的碳水化合物含量为66%,与玉米的碳水化合物含量(约73%)相当。这表明了C. vulgaris作为可发酵糖生产的替代底物的潜力。
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引用次数: 0
Tree or Shrub Jatropha curcas L.: Biofuel and Potential Herb 树或灌木麻疯树:生物燃料和潜在草本植物
Pub Date : 1900-01-01 DOI: 10.5958/0976-4763.2016.00012.X
Vikas Kumar, A. Tripathi, Pooja Tak, S. Chouhan
An attempt was made to update on review available literature on unique and potential herb of Jatropha curcas L. (Jatropha) for augmenting renewable energy source, genetic improvement and production of biodiesel from seed oil. Jatropha. curcas has spread beyond its original distribution because of its low moisture demands, pure hardiness, easy propagation, drought endurance, high oil content, low seed cost, short gestation period, rapid growth, adoption to wide agro-climatic condition, bushy/shrubby nature, tangible and intangible benefits of ecology and environment and it also used as a multifunctional plant for traditional medicine, bio-pesticide, land erosion control, live hedge, oil for lighting and soap making as well as alternative energy sources because it is non-toxic and biodegradable. Due to the concern on the availability of recoverable fossil fuel reserves and the environmental problems caused by the use those fossilfuels, considerableattention has been given to biodiesel production as an alternative to petrodiesel. Indeed, various important roles of Jatropha such as its application, extension, agroforestry systems, carbon sequestration, medicinal properties, agro-industrial solid waste, bio-fuels and by products, income and risks, which needs to be exploited well for its beneficial role in tropical environment. These issues are dealt herewith to observe its future scope to mitigate energy crisis, environmental management and sustainable productions.
本文对麻疯树(Jatropha curcas L.,简称麻疯树)独特草本植物和潜在草本植物在增加可再生能源、遗传改良和利用种子油生产生物柴油方面的研究进展进行了综述。麻疯树。麻瓜因其水分需求低、抗寒性强、易繁殖、耐旱、含油量高、种子成本低、孕育期短、生长快、适应广泛的农业气候条件、灌木性强、具有有形和无形的生态环境效益,已超越其原有的分布范围,成为传统医药、生物农药、防治水土流失、活树篱等多功能植物。用于照明和肥皂制造以及替代能源,因为它是无毒的和可生物降解的。由于对可采化石燃料储量的可获得性和使用这些化石燃料所引起的环境问题的关注,人们相当重视生产生物柴油作为石油柴油的替代品。麻疯树的应用、推广、农林复合系统、碳固存、药用特性、农用工业固体废弃物、生物燃料和副产品、收益和风险等多种重要作用需要充分利用,以发挥其在热带环境中的有益作用。在此处理这些问题,以观察其未来的范围,以减轻能源危机,环境管理和可持续生产。
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引用次数: 1
Nano-Copper Additive for Reducing NOx Emission in Soya Bean Biodiesel-Fuelled CI Engine 纳米铜添加剂用于降低大豆生物柴油发动机NOx排放
Pub Date : 1900-01-01 DOI: 10.5958/J.0976-4763.4.1.001
K. Balamurugan, A. Tamilvanan, M. Anbarasu, S. A. Mohamed, S. Srihari
Biodiesel usage in automotive engines is restricted due to its high nitrogen oxide (NOx) emission and low performance. Soya bean biodiesel (B10) with nano-copper particle as fuel additive was tested for performance and exhaust emission properties in a diesel engine. Nano-copper particles were synthesised using electrolysis method, and the characterisation (x-ray diffraction and scanning electron microscope) studies confirmed the particle sizes to be around 40 and 50 nm. Nano-copper particles were added to soya bean methyl ester through span 80 surfactant and further mixed with diesel as B10 blend. Various formulations (diesel, soya bean B10, soya bean B10+1.5% 30 nm Cu, soya bean B10+1.5% 42 nm Cu) were tested in a single-cylinder, water-cooled diesel engine and found that B10 with nano-copper particles show better engine performance and reduced NOx emission and smoke compared with other formulations.
生物柴油在汽车发动机中的使用受到限制,因为它的高氮氧化物(NOx)排放和低性能。以纳米铜颗粒为燃料添加剂,对大豆生物柴油(B10)在柴油机上的性能和废气排放特性进行了测试。采用电解法合成了纳米铜颗粒,并对其进行了表征(x射线衍射和扫描电子显微镜)研究,证实其粒径在40和50 nm左右。通过span 80表面活性剂将纳米铜颗粒加入到大豆甲酯中,再与柴油混合成B10共混物。在单缸水冷柴油机上对不同配方(柴油、大豆B10、大豆B10+1.5% 30 nm Cu、大豆B10+1.5% 42 nm Cu)进行了测试,发现纳米铜颗粒的B10比其他配方具有更好的发动机性能,降低了NOx排放和烟雾。
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引用次数: 36
Catalytic Applications of oxalic acid treated CuO/HZSM-5 in Methanol Conversion to Hydrocarbons 草酸处理CuO/HZSM-5在甲醇制烃中的催化应用
Pub Date : 1900-01-01 DOI: 10.5958/J.0976-3015.1.1.020
H. Zaidi, K. Pant
A series of CuO over HZSM-5 catalysts were prepared by wet impregnation technique followed by dealumination using oxalic acid. The loading of CuO over HZSM-5 was kept between 0 and 9 wt% and treated with oxalic acid. XRD, Surface area analyzer, metal trace analyzer and SEM techniques were used to characterize the catalysts. The higher yield of gasoline range hydrocarbons were obtained with the increase in weight % of CuO over HZSM5.Effect of run time on the hydrocarbon yields and methanol conversion was investigated. The activity of the catalyst decreased progressively with time on stream. Relatively lower coke deposition over HZSM-5 catalysts was observed compared to CuO impregnated HZSM-5 catalyst. The effect of run time was studied to investigate the effect oxalic acid treatment on catalyst stability.
采用湿浸渍法和草酸脱铝法制备了一系列CuO在HZSM-5催化剂上。在草酸处理下,HZSM-5的CuO负载保持在0 ~ 9 wt%之间。采用XRD、比表面积分析仪、金属痕量分析仪和扫描电镜等技术对催化剂进行了表征。在HZSM5上,随着CuO质量%的增加,汽油级烃类的收率提高。考察了运行时间对烃收率和甲醇转化率的影响。催化剂的活性随着生产时间的延长而逐渐降低。与CuO浸渍的HZSM-5催化剂相比,HZSM-5催化剂上的焦炭沉积相对较少。考察了草酸处理对催化剂稳定性的影响。
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引用次数: 1
Fabrication of Biodiesel Batch Reactor Experimental Setup 生物柴油间歇式反应器实验装置的研制
Pub Date : 1900-01-01 DOI: 10.5958/0976-4763.2021.00006.4
Sangeeta Kanakraj, S. Dixit, A. Rehman
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引用次数: 0
Effect of Fungal Biotreatment Method for Lignocellulosic Ethanol Production from Areca Nut (Areca catechu L.) Husk Using Yeasts and Zymomonas mobilis NCIM 2915 真菌生物处理方法对槟榔果木质纤维素乙醇生产的影响利用酵母和活动单胞菌NCIM 2915制备谷壳
Pub Date : 1900-01-01 DOI: 10.5958/0976-4763.2016.00011.8
Naveen Kumar Kudure Jayanna, Thippeswamy Basaiah, K. Madappa
Sustainable development is the underpinning principle in the panacea for almost every environmental concern. Generation of energy from the biomass can solve the purpose of environmentalist. Bioethanol and biodiesel that comprises biofuel is one such form of green energy. The major drivers for bioethanol production in India are energy security, slower potential for global warming and converting waste to energy. For bioethanol lignocellulosic, biomass is the most abundant renewable resource that can serve as substrate for its production. Bioconversion offers a cheap and safe method of not only disposing the agricultural residues, but also it has the potential to convert lignocellulosic wastes into usable forms, such as reducing sugars that could be used for ethanol production. This paper reports a preliminary study on the microbial pretreatment and fermentation of the areca nut husk. A combination of Aspergillus terreus and Phanerochaete chrysosporium NCIM 1197 (AT + PC) obtained from screening was used for pretreatment and, yeasts and bacterium Zymomonas mobilis NCIM 2915 were used for carrying out fermentation. Z. mobilis NCIM 2915 was showed maximum ethanol production after fermentation from areca nut husk as followed by Pichia stipitis NCIM 3498, Saccharomyces cerevisiae NCIM 3095 and Candida shehatae NCIM 3500. Hence, fungal pretreatment by cellulolytic fungi was more effective for ethanol production. Areca nut husk was revealed as a suitable substrate for ethanol production.
可持续发展是解决几乎所有环境问题的灵丹妙药的基本原则。利用生物质发电可以解决环境保护的问题。由生物燃料组成的生物乙醇和生物柴油就是这样一种绿色能源。印度生物乙醇生产的主要驱动因素是能源安全、减缓全球变暖的潜力以及将废物转化为能源。对于生物乙醇木质纤维素,生物质是最丰富的可再生资源,可以作为其生产的底物。生物转化提供了一种廉价而安全的方法,不仅可以处理农业残留物,而且还具有将木质纤维素废物转化为可用形式的潜力,例如可用于乙醇生产的还原糖。本文对槟榔果皮的微生物预处理和发酵进行了初步研究。筛选得到的土曲霉和黄孢平革菌NCIM 1197 (AT + PC)组合进行预处理,酵母和活动单胞菌NCIM 2915进行发酵。槟榔果皮发酵产乙醇量最大的是Z. mobilis NCIM 2915,其次是毕赤酵母NCIM 3498、酿酒酵母NCIM 3095和假丝酵母NCIM 3500。因此,纤维素水解真菌预处理对乙醇生产更为有效。槟榔果壳是生产乙醇的合适底物。
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引用次数: 0
Comparison and Evaluation of Two Pretreatment Processes for Enhancing Enzymatic Saccharification of Sweet Sorghum Bagasse 促进甜高粱甘蔗渣酶解糖化的两种预处理工艺比较与评价
Pub Date : 1900-01-01 DOI: 10.5958/J.0976-3015.1.1.003
Aditi Banerji, M. Balakrishnan, V. Kishore
With world reserves of petroleum fast depleting and growing environmental degradation concerns, ethanol has emerged as an important alternative transportation fuel. Lignocellulosic biomass is a readily available resource that can be used for ethanol production after breaking the cellulose and hemicellulose fractions into their component monosaccharides. In this study, we investigated the release of reducing sugars from sweet sorghum bagasse using a two – stage chemical pretreatment (dilute acid hydrolysis followed by alkaline-peroxide delignification) and steam pretreatment; the solid residue obtained in both cases were further subjected to enzymatic saccharification. The results show that steam pretreatment followed by enzymatic saccharification is more promising. It released 75.7% of the total sugars originally present in the bagasse, compared to 66.1% obtained with twostage chemical pretreatment-enzymatic saccharification.
随着世界石油储量的快速消耗和环境恶化问题的日益严重,乙醇已成为一种重要的替代运输燃料。木质纤维素生物质是一种容易获得的资源,在将纤维素和半纤维素组分分解成单糖组分后,可用于乙醇生产。在这项研究中,我们研究了甜高粱甘蔗渣中还原糖的释放采用两阶段化学预处理(稀酸水解,碱-过氧化物脱木质素)和蒸汽预处理;在这两种情况下得到的固体残渣进一步进行酶糖化。结果表明,蒸汽预处理后再进行酶解糖化是较有前途的方法。它释放了甘蔗渣中最初存在的总糖的75.7%,而两阶段化学预处理-酶糖化获得的总糖为66.1%。
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引用次数: 3
Biodiesel Policies for India: Achieving Optimal Socio-Economic and Environmental Impact 印度生物柴油政策:实现最佳社会经济和环境影响
Pub Date : 1900-01-01 DOI: 10.5958/J.0976-3015.1.1.022
T. Altenburg
Production of biodiesel from tree-borne oilseeds is often praised as a solution to many problems of rural development in India: employment generation on wastelands; afforestation of eroded land; soil conservation; a new source of energy, etc. Whether and to what extent these goals are achieved depends on the selected oilseed species, and, more importantly, the mode of production. We found many different ways of organising the biodiesel value chain that have emerged on the basis of varying local conditions and power relations among interest groups in five Indian states. Each mode has different socio-economic and environmental impacts, and there are manifold trade-offs. The article highlights the pros and cons of different modes of value chain organisation. It also discusses policies to improve their development effects. To choose the right policy-mix, policymakers need to be clear about their objectives and potential trade-offs. The article further emphasizes the need for better research before poor farmers are encouraged to plant oilseeds; and it discusses a number of supply and demand-side policies to accelerate the use of oilseeds for biodiesel in India.
从树生油籽生产生物柴油经常被称赞为解决了印度农村发展的许多问题:在荒地上创造就业机会;侵蚀土地造林;水土保持;新能源等。是否以及在多大程度上实现这些目标取决于所选择的油籽品种,更重要的是,取决于生产方式。我们发现了许多组织生物柴油价值链的不同方式,这些方式是基于印度五个邦不同的当地条件和利益集团之间的权力关系而出现的。每种模式都有不同的社会经济和环境影响,并且存在多方面的权衡。本文着重分析了不同价值链组织模式的优缺点。本文还讨论了提高其发展效果的政策。为了选择正确的政策组合,政策制定者需要清楚他们的目标和潜在的权衡。文章进一步强调,在鼓励贫困农民种植油籽之前,需要进行更好的研究;它还讨论了一些供需方面的政策,以加速在印度使用油籽生产生物柴油。
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引用次数: 3
Ethanol Blended Fuel in India: An Overview 印度的乙醇混合燃料:综述
Pub Date : 1900-01-01 DOI: 10.5958/J.0976-3015.1.2.026
Mahesh Saini, N. Garg, Ashutosh Kumar Singh, A. Tyagi, U. Niyogi, R. K. Khandal
Ethanol produced from renewable resources is being considered globally as the most prominent and possible substitute for fossil fuel. It is being produced by fermentation of raw materials obtained from various renewable resources like Sugarcane, Corn and Sweet Sorghum all over the world. Ethanol is mainly blended with gasoline in different ratios and commonly known as ‘Gasohol’. This special use has escalated the global production of ethanol by 85% in 2006 from 2002. Oil embargo of 1973 forced Brazil to initiate and implement the Bio-ethanol for mandatory use in automotive fuel upto the extent of 100%. For this to happen, the flexi-fuel vehicles were also introduced that can run both on petrol and E100 (Bio-ethanol). Other countries like US, Europe and India followed this successful model to achieve the objectives of self reliance in fuel. At present in India ethanol is blended @ 5% in petrol. Ethanol in fuel increases its oxygen content and has replaced the use of MTBE and ETBE. Various studies conducted all over the world have revealed that ethanol blending in petrol at different ratios has remarkably reduced the emission of GHG from vehicles. Ethanol has also been used along with diesel, but comparatively ethanol blended petrol is more acceptable than with diesel because later requires the admixture of surfactants to keep the blend stable, which increases the cost of the fuel. Even, having so much benefits of ethanol blending, its use is limited by various technical factors like, degradation of non-metallic components of engine, decrease in fuel lubricity, viscosity and calorific value of the fuel, etc. The other Non-technical factors also plays major role, which are elaborately discussed in this paper. Government of India in the year 2002 issued the first national Biofuel policy, in order to use bio-ethanol and bio-diesel as a fuel for transport. However, certain factors hindered the successful execution of ethanol use as a transport fuel. This paper present the studies conducted to find out the reasons for limited success of especially ethanol blended fuel in India. The outcome of this study would serve as the base reference for the policy makers to devise strategies to achieve the indicative target of recent Biofuel Policy released on September 11, 2008 mandating 20 % blending of biofuels by 2017.
从可再生资源生产的乙醇被全球认为是化石燃料最重要和最可能的替代品。它是由世界各地的甘蔗、玉米、甜高粱等各种可再生资源的原料发酵而成的。乙醇主要与汽油按不同比例混合,通常称为“汽油醇”。这种特殊用途使2006年全球乙醇产量比2002年提高了85%。1973年的石油禁运迫使巴西启动并实施生物乙醇强制用于汽车燃料,达到100%的程度。为了实现这一目标,灵活燃料汽车也被引入,既可以使用汽油,也可以使用E100(生物乙醇)。美国、欧洲和印度等其他国家效仿这一成功模式,实现了燃料自给自足的目标。目前在印度,乙醇在汽油中的混合比例为5%。燃料中的乙醇增加了其氧含量,并取代了MTBE和ETBE的使用。世界各地进行的各种研究表明,在汽油中以不同比例混合乙醇显著减少了车辆的温室气体排放。乙醇也曾与柴油一起使用,但相对而言,乙醇混合汽油比柴油混合汽油更容易被接受,因为后者需要表面活性剂的混合物来保持混合物的稳定性,这增加了燃料的成本。即使乙醇混合有这么多的好处,它的使用也受到各种技术因素的限制,如发动机非金属部件的降解,燃料的润滑性,粘度和热值的降低等。其他非技术因素也起着重要作用,本文对此进行了详细讨论。印度政府于2002年发布了第一个国家生物燃料政策,以使用生物乙醇和生物柴油作为运输燃料。然而,某些因素阻碍了乙醇作为运输燃料的成功实施。本文提出的研究,以找出有限的成功的原因,特别是乙醇混合燃料在印度。这项研究的结果将作为政策制定者制定战略的基础参考,以实现2008年9月11日发布的生物燃料政策的指示性目标,该政策要求到2017年生物燃料的混合比例达到20%。
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引用次数: 9
Experimental Investigations on Performance and Emissions of a C.I. Engine Fuelled With Milk Dairy Waste Scum Oil Biodiesel 以乳业废渣油生物柴油为燃料的内燃机性能与排放试验研究
Pub Date : 1900-01-01 DOI: 10.5958/0976-4763.2015.00005.7
H. V. Srikanth, J. Venkatesh, G. Sharanappa
The present research work proposes to study the transesterification process of milk dairy waste scum oil and its suitability as a fuel for CI engine by conducting performance and emission tests. This study was carried out in two steps, production of milk dairy waste scum biodiesel followed by performance and emission tests on a single cylinder, four-stroke CI engine with water cooled system at a fixed engine speed of 1500 rpm and at different load conditions. The impact of milk dairy waste scum oil biodiesel and its blends as a fuel on brake-specific fuel consumption, brake thermal efficiency and brake-specific energy consumption and exhaust gas emissions has been investigated and presented. From the experimental results it is concluded that the performance and emission characteristics of B20 are satisfactory compared to all other blends. Hence, blend B20 can be used as an alternative fuel source in CI engine without any modifications in engine hardware.
本研究拟通过性能试验和排放试验,研究乳业废渣油的酯交换过程及其作为内燃机燃料的适用性。本研究分两个步骤进行,首先生产乳业废渣生物柴油,然后在一台水冷系统的单缸四冲程CI发动机上,在发动机固定转速1500转/分和不同负载条件下进行性能和排放测试。研究了牛奶、乳业废渣、油脂、生物柴油及其混合燃料对制动比油耗、制动热效率、制动比能耗和废气排放的影响。实验结果表明,与其他混合燃料相比,B20的性能和排放特性是令人满意的。因此,混合B20可作为CI发动机的替代燃料源,无需对发动机硬件进行任何修改。
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引用次数: 1
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Journal of Biofuels
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