Pub Date : 2022-04-26DOI: 10.37290/ctnr2641-452x.20:623-629
Juanjuan Cai, Lingling Su, Weiwei Luo
Resistance of cervical cancer to cisplatin chemotherapy compromises the therapeutic efficacy of the treatment. Polyphyllin I, a bioactive compound extracted from rhizoma of Paris polyphyllin, displays a selective inhibitory effect on a variety of tumor cells. Furthermore, it potentiates the chemosensitivity of cisplatin against cisplatin resistant gastric cancer cells and lung adenocarcinoma. However, its role in modulating chemosensitivity of cisplatin resistant cervical cancer cells remains unexplored. To this end, the repressive effect of polyphyllin I on proliferation, invasion and migration of cisplatin resistant cervical cancer cells compared to the parental cervical cancer cells as well as downregulation of mitochondrial membrane potential of cisplatin resistant cervical cancer cells, as well as upregulation of protein kinase R-like ER kinase/translation initiation factor 2-alpha kinase 3, protein-kinase and site-specific endoribonuclease/inositol-requiring enzyme 1, binding immunoglobulin protein, and C/EBP homologous transcription factor proteins. In conclusion, polyphyllin I stimulate mitochondrial dysfunction and endoplasmic reticulum stress to attenuate cisplatin resistance of cervical cancer.
{"title":"Polyphyllin I Inhibits Cisplatin Resistance of Cervical Cancer Cells by Inducing Mitochondrial Dysfunction and Endoplasmic Reticulum Stress","authors":"Juanjuan Cai, Lingling Su, Weiwei Luo","doi":"10.37290/ctnr2641-452x.20:623-629","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.20:623-629","url":null,"abstract":"Resistance of cervical cancer to cisplatin chemotherapy compromises the therapeutic efficacy of the treatment. Polyphyllin I, a bioactive compound extracted from rhizoma of Paris polyphyllin, displays a selective inhibitory effect on a variety of tumor cells. Furthermore, it potentiates the chemosensitivity of cisplatin against cisplatin resistant gastric cancer cells and lung adenocarcinoma. However, its role in modulating chemosensitivity of cisplatin resistant cervical cancer cells remains unexplored. To this end, the repressive effect of polyphyllin I on proliferation, invasion and migration of cisplatin resistant cervical cancer cells compared to the parental cervical cancer cells as well as downregulation of mitochondrial membrane potential of cisplatin resistant cervical cancer cells, as well as upregulation of protein kinase R-like ER kinase/translation initiation factor 2-alpha kinase 3, protein-kinase and site-specific endoribonuclease/inositol-requiring enzyme 1, binding immunoglobulin protein, and C/EBP homologous transcription factor proteins. In conclusion, polyphyllin I stimulate mitochondrial dysfunction and endoplasmic reticulum stress to attenuate cisplatin resistance of cervical cancer.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45059084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-21DOI: 10.37290/ctnr2641-452x.20:648-653
Xiaokai Yang, Shuai Liu, Z. Wang
Inhibition of bone loss is one of the ways to alleviate motor dysfunction caused by spinal cord injury, a lifelong and lifechanging injury. Isoflavone from legumes that include ononin, Calycosin-7-O-β-Glucoside, and others have been shown to have potential for the treatment of orthopedic diseases. While there are potentials for ononin to improve the motor function of spinal cord injury, studies on the subject are lacking. To this end, we examined the ameliorative role of ononin in an animal model of spinal cord injury. Our results show the ononin reduced inflammatory response, decreased femoral bone loss, and promoted osteoblast formation in rats with spinal cord injury through promotion of adenosine 5′′-monophosphate-activated protein kinase signaling pathway.
脊髓损伤是一种终身性损伤，抑制骨质流失是减轻脊髓损伤所致运动功能障碍的途径之一。从豆科植物中提取的异黄酮，包括芒草苷，花萼异黄酮-7- o -β-葡萄糖苷等，已被证明具有治疗骨科疾病的潜力。虽然茴香素有改善脊髓损伤运动功能的潜力，但这方面的研究还很缺乏。为此，我们在脊髓损伤动物模型中检测了苦参素的改善作用。我们的研究结果表明，木柄草苷通过促进腺苷5′-单磷酸激活的蛋白激酶信号通路，降低了脊髓损伤大鼠的炎症反应，减少了股骨骨丢失，促进了成骨细胞的形成。
{"title":"Ononin Alleviates Sublesional Bone Loss and Inflammation Following Spinal Cord Injury in Rats by Regulating Adenosine 5′-Monophosphate-Activated Protein Kinase Signaling Pathway","authors":"Xiaokai Yang, Shuai Liu, Z. Wang","doi":"10.37290/ctnr2641-452x.20:648-653","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.20:648-653","url":null,"abstract":"Inhibition of bone loss is one of the ways to alleviate motor dysfunction caused by spinal cord injury, a lifelong and lifechanging injury. Isoflavone from legumes that include ononin, Calycosin-7-O-β-Glucoside, and others have been shown to have potential for the treatment of orthopedic diseases. While there are potentials for ononin to improve the motor function of spinal cord injury, studies on the subject are lacking. To this end, we examined the ameliorative role of ononin in an animal model of spinal cord injury. Our results show the ononin reduced inflammatory response, decreased femoral bone loss, and promoted osteoblast formation in rats with spinal cord injury through promotion of adenosine 5′′-monophosphate-activated protein kinase signaling pathway.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44199691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-13DOI: 10.37290/ctnr2641-452x.20:720-724
N. Todorovic, V. Štajer, A. Milovančev, Danijela Musulin Banjanin, Milijana Celarevic, L. Rátgéber, J. Betlehem, P. Ács, Ron Kramer, Alexander Nicolaidis, P. Drid, N. Maksimović, S. Ostojić
The main objective of this randomized controlled trial was to analyze the acute effects of a nitrate-based nutritional formula on peripheral oxygen saturation (SpO2), patient-reported outcomes, and safety indices in patients with acute COVID-19 infection. The participants completing the protocol for the trial (n = 71) were allocated in a double-blind design to receive either a multicomponent nutritional formula (containing 1200 mg of potassium nitrate, 200 mg of magnesium, 50 mg of zinc, and 1000 mg of citric acid), or a placebo (2.5 g of inulin) during a 72-h monitoring period. All participants were requested to take an intervention (four capsules; weight of each capsule was ~2.5 g) every 4 h during the intervention period. A two-way mixed model ANOVA with repeated measures revealed a significant difference in SpO2 between interventions (P = 0.007). As many as 30 out of 38 patients (78.9%) receiving nitrate-based nutritional formula, who all started the treatment with SpO2 ≤ 95%, finished the intervention with SpO2 of at least 95% at 72-h follow-up, whereas in the placebo group, 13 patients out of 33 (39.4%) finished the trial with SpO2 of at least 95% (P < 0.05). The hospital admission rate after the 72-h follow-up was 21.1% in patients receiving nitrate-based formula compared to 33.3% in the placebo group (P = 0.25). The nitrate-based formula might be recognized as a potent strategy to tackle hypoxemia and concomitant features of the COVID-19 pandemic. In conclusion, it appears that the novel nitrated-based nutritional formulation described here was able to improve oxygen saturation and clinical outcomes in COVID-19 patients. Although the exact mechanism of action remains unknown, the nitrate-based formula might be recognized as a potent, safe, and convenient strategy to tackle hypoxemia and concomitant features of the COVID-19 pandemic.
{"title":"The Effects of Nitrate-Based Nutritional Formula on Oxygen Saturation and Patient-Reported Outcomes in COVID-19 Patients: A Randomized Controlled Trial","authors":"N. Todorovic, V. Štajer, A. Milovančev, Danijela Musulin Banjanin, Milijana Celarevic, L. Rátgéber, J. Betlehem, P. Ács, Ron Kramer, Alexander Nicolaidis, P. Drid, N. Maksimović, S. Ostojić","doi":"10.37290/ctnr2641-452x.20:720-724","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.20:720-724","url":null,"abstract":"The main objective of this randomized controlled trial was to analyze the acute effects of a nitrate-based nutritional formula on peripheral oxygen saturation (SpO2), patient-reported outcomes, and safety indices in patients with acute COVID-19 infection. The participants completing the protocol for the trial (n = 71) were allocated in a double-blind design to receive either a multicomponent nutritional formula (containing 1200 mg of potassium nitrate, 200 mg of magnesium, 50 mg of zinc, and 1000 mg of citric acid), or a placebo (2.5 g of inulin) during a 72-h monitoring period. All participants were requested to take an intervention (four capsules; weight of each capsule was ~2.5 g) every 4 h during the intervention period. A two-way mixed model ANOVA with repeated measures revealed a significant difference in SpO2 between interventions (P = 0.007). As many as 30 out of 38 patients (78.9%) receiving nitrate-based nutritional formula, who all started the treatment with SpO2 ≤ 95%, finished the intervention with SpO2 of at least 95% at 72-h follow-up, whereas in the placebo group, 13 patients out of 33 (39.4%) finished the trial with SpO2 of at least 95% (P < 0.05). The hospital admission rate after the 72-h follow-up was 21.1% in patients receiving nitrate-based formula compared to 33.3% in the placebo group (P = 0.25). The nitrate-based formula might be recognized as a potent strategy to tackle hypoxemia and concomitant features of the COVID-19 pandemic. In conclusion, it appears that the novel nitrated-based nutritional formulation described here was able to improve oxygen saturation and clinical outcomes in COVID-19 patients. Although the exact mechanism of action remains unknown, the nitrate-based formula might be recognized as a potent, safe, and convenient strategy to tackle hypoxemia and concomitant features of the COVID-19 pandemic.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47690127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-13DOI: 10.37290/ctnr2641-452x.20:710-715
Zhipeng Chen, Qiaoya He, Yi Yuan
The present study was designed to investigate the role of Taohong Siwu decoction in postoperative recovery from unicompartmental knee arthroplasty. Eighty gonitis patients undergoing unicompartmental knee arthroplasty were recruited and divided into study and control groups using the random number table method. The control group was treated with analgesics and performed routine functional exercise after the operation as per the recommended standard of care, while those in the study group were additionally treated with Taohong Siwu decoction. The total effectiveness rate for the study group was 95.00% (38/40), which was significantly higher than the control group’s 70.00% (28/40). The study group shows better exercise ability than the control group. While after the operation, the skin temperature in two groups increased and then decreased, the skin temperature in the study group was significantly lower than that in the control group. The Lysholm scores, a measure of knee-specific symptoms, were significantly higher in the study group compared to the control. The adverse reaction rates in the study and control groups were 5.00% and 7.25%, respectively, and the difference was not statistically significant. Taohong Siwu decoction can effectively help relieve pain, reduce the inflammatory cytokine content, elevate the knee joint function, improve the exercise ability, and improve the living ability of gonitis patients undergoing unicompartmental knee arthroplasty.
{"title":"The Clinical Efficacy of Taohong Siwu Decoction in Facilitating Recovery from Postoperative Unicompartmental Knee Arthroplasty","authors":"Zhipeng Chen, Qiaoya He, Yi Yuan","doi":"10.37290/ctnr2641-452x.20:710-715","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.20:710-715","url":null,"abstract":"The present study was designed to investigate the role of Taohong Siwu decoction in postoperative recovery from unicompartmental knee arthroplasty. Eighty gonitis patients undergoing unicompartmental knee arthroplasty were recruited and divided into study and control groups using the random number table method. The control group was treated with analgesics and performed routine functional exercise after the operation as per the recommended standard of care, while those in the study group were additionally treated with Taohong Siwu decoction. The total effectiveness rate for the study group was 95.00% (38/40), which was significantly higher than the control group’s 70.00% (28/40). The study group shows better exercise ability than the control group. While after the operation, the skin temperature in two groups increased and then decreased, the skin temperature in the study group was significantly lower than that in the control group. The Lysholm scores, a measure of knee-specific symptoms, were significantly higher in the study group compared to the control. The adverse reaction rates in the study and control groups were 5.00% and 7.25%, respectively, and the difference was not statistically significant. Taohong Siwu decoction can effectively help relieve pain, reduce the inflammatory cytokine content, elevate the knee joint function, improve the exercise ability, and improve the living ability of gonitis patients undergoing unicompartmental knee arthroplasty.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42873228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-26DOI: 10.37290/ctnr2641-452x.20:691-697
Xianqing Meng, Haiyang Sun, Fei-hu Zhang, Yuehua Jiang, Kejian Li, Zhongping Ma, Di Wu, Yitao Xue, H. Hao
Gynostemma pentaphyllum (Thunb.) Makino is a Chinese herb known to alleviate hyperlipidemia and obesity. In the present study, the effect and the mechanism of the effect of gypenoside IX, a bioactive constituent of G. pentaphyllum on the obesity-related hypertension in rats has been explored. The rats were fed a high fat diet to induce obesity leading to hypertension. Treatment of these hypertensive rats with gypenoside IX resulted in decreased systolic and diastolic blood pressure, body weight, and alleviation of pathological manifestations in myocardium and aorta. Furthermore, gypenoside IX antagonized the high fat diet-induced relative increase in the expressions of angiotensin-converting enzyme, angiotensin II receptor type 1 and 2 proteins, and the B-type natriuretic peptide and atrial natriuretic peptide messenger RNAs. However, the concentrations of angiotensin II, adrenaline, and norepinephrine increased, whereas that of cyclic guanosine monophosphate decreased. The levels of malondialdehyde increased and that of reduced glutathione, superoxide dismutase, and catalase decreased in high fat diet rats that were reversed by the gypenoside IX treatment. There was a significant increase in the relative protein levels for phospho-extracellular signal-regulated kinase/extracellular signal-regulated kinase, phospho-c-Jun NH2-terminal kinase/c-Jun NH2-terminal kinase, and p-p38/p-38 that was ameliorated by gypenoside IX. In conclusion, gypenoside IX inhibits the renin–angiotensin system activation and oxidative stress through the inactivation of the mitogen-activated protein kinase pathway in obesity-mediated hypertension in rats.
{"title":"Gypenoside IX Ameliorates Hypertension Induced by Obesity via Inactivation of the Mitogen-Activated Protein Kinase Pathway","authors":"Xianqing Meng, Haiyang Sun, Fei-hu Zhang, Yuehua Jiang, Kejian Li, Zhongping Ma, Di Wu, Yitao Xue, H. Hao","doi":"10.37290/ctnr2641-452x.20:691-697","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.20:691-697","url":null,"abstract":"Gynostemma pentaphyllum (Thunb.) Makino is a Chinese herb known to alleviate hyperlipidemia and obesity. In the present study, the effect and the mechanism of the effect of gypenoside IX, a bioactive constituent of G. pentaphyllum on the obesity-related hypertension in rats has been explored. The rats were fed a high fat diet to induce obesity leading to hypertension. Treatment of these hypertensive rats with gypenoside IX resulted in decreased systolic and diastolic blood pressure, body weight, and alleviation of pathological manifestations in myocardium and aorta. Furthermore, gypenoside IX antagonized the high fat diet-induced relative increase in the expressions of angiotensin-converting enzyme, angiotensin II receptor type 1 and 2 proteins, and the B-type natriuretic peptide and atrial natriuretic peptide messenger RNAs. However, the concentrations of angiotensin II, adrenaline, and norepinephrine increased, whereas that of cyclic guanosine monophosphate decreased. The levels of malondialdehyde increased and that of reduced glutathione, superoxide dismutase, and catalase decreased in high fat diet rats that were reversed by the gypenoside IX treatment. There was a significant increase in the relative protein levels for phospho-extracellular signal-regulated kinase/extracellular signal-regulated kinase, phospho-c-Jun NH2-terminal kinase/c-Jun NH2-terminal kinase, and p-p38/p-38 that was ameliorated by gypenoside IX. In conclusion, gypenoside IX inhibits the renin–angiotensin system activation and oxidative stress through the inactivation of the mitogen-activated protein kinase pathway in obesity-mediated hypertension in rats.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43145284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-23DOI: 10.37290/ctnr2641-452x.20m:m17-m23
Vijayamahantesh K. Tandur, M. N. Inamdar, S. M. Asdaq, S. Rabbani, A. Alamri, M. Alhomrani, W. Alsanie
Herbal medicine has been used since ancient times to treat chronic constipation. The present study has investigated the gut stimulatory activity of Andrographis peniculata, an herbal medicine common to many parts of the globe. Results of these studies show a dose-dependent increase in the contraction of smooth muscle from isolated rat colon, guinea pig ileum, and rabbit jejunum by A. peniculata extracts. Furthermore, it enhanced intestinal fecal transit in mice. Additional studies into its action suggested the involvement of a cholinergic mechanism.
{"title":"Evaluation of the Laxative-Like Activity of Andrographis Paniculata","authors":"Vijayamahantesh K. Tandur, M. N. Inamdar, S. M. Asdaq, S. Rabbani, A. Alamri, M. Alhomrani, W. Alsanie","doi":"10.37290/ctnr2641-452x.20m:m17-m23","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.20m:m17-m23","url":null,"abstract":"Herbal medicine has been used since ancient times to treat chronic constipation. The present study has investigated the gut stimulatory activity of Andrographis peniculata, an herbal medicine common to many parts of the globe. Results of these studies show a dose-dependent increase in the contraction of smooth muscle from isolated rat colon, guinea pig ileum, and rabbit jejunum by A. peniculata extracts. Furthermore, it enhanced intestinal fecal transit in mice. Additional studies into its action suggested the involvement of a cholinergic mechanism.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43785749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-22DOI: 10.37290/ctnr2641-452x.20:581-587
Ying Yang, Tianwei Gao
Use of alantolactone in traditional Chinese medicine has been reported in a wide variety of diseases. To explore the role of alantolactone in chronic heart failure, its effect on doxorubicin treated H9c2 cells as a model was examined. The results of our studies show doxorubicin treatment decreased cell viability, enabled induction of secretion of proinflammatory cytokines, increased expression of malondialdehyde and decreased expression of superoxide dismutase, and enabled downregulation of nuclear factor erythroid 2-related factor 2 and heme oxygenase-1 pathways. Furthermore, all the reported effects of doxorubicin were reversed by alantolactone. In conclusion, alantolactone exhibits anti-inflammatory and antioxidative effects on doxorubicin treated H9c2 cells through activation of nuclear factor erythroid 2-related factor 2/heme oxygenase-1 and inactivation of nuclear factor-kappa B pathway. Therefore, alantolactone might be a potential agent for the prevention of doxorubicin induced chronic heart failure.
{"title":"Alantolactone Suppresses Inflammation and Oxidative Stress in Doxorubicin Induced H9c2 Cells","authors":"Ying Yang, Tianwei Gao","doi":"10.37290/ctnr2641-452x.20:581-587","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.20:581-587","url":null,"abstract":"Use of alantolactone in traditional Chinese medicine has been reported in a wide variety of diseases. To explore the role of alantolactone in chronic heart failure, its effect on doxorubicin treated H9c2 cells as a model was examined. The results of our studies show doxorubicin treatment decreased cell viability, enabled induction of secretion of proinflammatory cytokines, increased expression of malondialdehyde and decreased expression of superoxide dismutase, and enabled downregulation of nuclear factor erythroid 2-related factor 2 and heme oxygenase-1 pathways. Furthermore, all the reported effects of doxorubicin were reversed by alantolactone. In conclusion, alantolactone exhibits anti-inflammatory and antioxidative effects on doxorubicin treated H9c2 cells through activation of nuclear factor erythroid 2-related factor 2/heme oxygenase-1 and inactivation of nuclear factor-kappa B pathway. Therefore, alantolactone might be a potential agent for the prevention of doxorubicin induced chronic heart failure.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46406246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-20DOI: 10.37290/ctnr2641-452x.20:654-661
Chao Liu, Suqian Zhao, Bao Zhong, Y. Piao, F. Liang, Wei Cui, Fenglin Li
Morchella esculenta L. is a rare and the most valuable edible mushrooms. The polysaccharides of M. esculenta L. boast high nutrition and health values. This paper describes microwave extraction of the polysaccharides of M. esculenta L. by ultrasonic microwave. The optimal extraction conditions, optimized by single factor experiments and response surface method, were determined as follows: the ultrasonic power is 250 W, the microwave power is 220 W, and the extraction time is 7 min with a yield of polysaccharides at 4.425%. The crude polysaccharides of M. esculenta L. at 4 mg/mL exhibited hydroxyl radical, 2-Diphenyl-1-picrylhydrazyl radical and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging rates at 76.70%, 60.73%, and 80.87%, respectively. Its Fe2+ chelating rates increased from 45.63% to 82.50%, as the polysaccharide concentration increased from 0.5 to 4.0 mg/mL.
{"title":"Ultrasound-Microwave Co-Extraction and Antioxidant Activity Analysis of Polysaccharides from Morchella Esculenta L. Mycelium Obtained through Submerged Fermentation","authors":"Chao Liu, Suqian Zhao, Bao Zhong, Y. Piao, F. Liang, Wei Cui, Fenglin Li","doi":"10.37290/ctnr2641-452x.20:654-661","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.20:654-661","url":null,"abstract":"Morchella esculenta L. is a rare and the most valuable edible mushrooms. The polysaccharides of M. esculenta L. boast high nutrition and health values. This paper describes microwave extraction of the polysaccharides of M. esculenta L. by ultrasonic microwave. The optimal extraction conditions, optimized by single factor experiments and response surface method, were determined as follows: the ultrasonic power is 250 W, the microwave power is 220 W, and the extraction time is 7 min with a yield of polysaccharides at 4.425%. The crude polysaccharides of M. esculenta L. at 4 mg/mL exhibited hydroxyl radical, 2-Diphenyl-1-picrylhydrazyl radical and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging rates at 76.70%, 60.73%, and 80.87%, respectively. Its Fe2+ chelating rates increased from 45.63% to 82.50%, as the polysaccharide concentration increased from 0.5 to 4.0 mg/mL.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44181477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-16DOI: 10.37290/ctnr2641-452x.20:561-566
D. Yu, Jin Xu, Ya Chen, B. Shi
The present study has examined the effect of procyanidin A1 on insulin resistance and oxidative stress in palmitic acid treated HepG2 cells. The HepG2 cells were pretreated with procyanidin A1 and then with palmitic acid. The cell viability and the levels of catalase, superoxide dismutase, glutathione peroxidase, and reactive oxygen were used to assess oxidative stress. Treatment with palmitic acid significantly reduced cell viability that was alleviated by cotreatment with procyanidin A1. In addition, procyanidin A1 restored the palmitic acid induced insulin resistance and oxidative stress in the HepG2 cells. Furthermore, inactivation of P38 MAPK and JNK signaling pathways was closely associated with the procyanidin A1-regulated resistance and oxidative stress in palmitic acid treated HepG2 cells. In conclusion, procyanidin A1 inhibits insulin resistance and oxidative stress in palmitic acid treated HepG2 cells by regulating the P38 MAPK and JNK signaling pathways.
{"title":"Procyanidin A1 Inhibits Insulin Resistance and Oxidative Stress in Palmitic Acid Treated HepG2 Cells","authors":"D. Yu, Jin Xu, Ya Chen, B. Shi","doi":"10.37290/ctnr2641-452x.20:561-566","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.20:561-566","url":null,"abstract":"The present study has examined the effect of procyanidin A1 on insulin resistance and oxidative stress in palmitic acid treated HepG2 cells. The HepG2 cells were pretreated with procyanidin A1 and then with palmitic acid. The cell viability and the levels of catalase, superoxide dismutase, glutathione peroxidase, and reactive oxygen were used to assess oxidative stress. Treatment with palmitic acid significantly reduced cell viability that was alleviated by cotreatment with procyanidin A1. In addition, procyanidin A1 restored the palmitic acid induced insulin resistance and oxidative stress in the HepG2 cells. Furthermore, inactivation of P38 MAPK and JNK signaling pathways was closely associated with the procyanidin A1-regulated resistance and oxidative stress in palmitic acid treated HepG2 cells. In conclusion, procyanidin A1 inhibits insulin resistance and oxidative stress in palmitic acid treated HepG2 cells by regulating the P38 MAPK and JNK signaling pathways.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43986889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-11DOI: 10.37290/ctnr2641-452x.20:675-684
D. Chatziharalambous, D. Sarris, K. Gkatzionis, A. Koutelidakis
Wheat flour is the major constituent of bakery products consumed as everyday snacks worldwide. However, due to its poor nutritional value, high gluten content, and rapid digestion has been linked to the onset of celiac disease, obesity, diabetes type II, and metabolic syndrome. Therefore, alternative flours from olive and grape by-products as well as pulses, such as chickpea, with higher nutritional value and prebiotic function have been exploited to enrich those products and possibly confer several health benefits due to their high bioactivity. To this end, the bioactivity of grape and olive by-products as well as chickpea is described. This study emphasizes the crucial function of the related compounds in human nutrition and metabolism, particularly in relation to post-prandial glycemia and lipidemia, as their acute perturbation is linked to the development of metabolic diseases. Dietary supplementation with either chickpea, olive or grape pomace extracts or powders in in vivo and in vitro studies demonstrated beneficial properties in terms of glucose and lipid metabolism management. Moreover, hepato- and cardio-protective, antioxidant, and anti-inflammatory properties were observed. Furthermore, fortification of bakery snacks with chickpea, olive, and grape pomace flours demonstrated promising results in improving the lipid and glycemic profiles in human intervention studies.
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