首页 > 最新文献

Current Topics in Nutraceutical Research最新文献

英文 中文
Detection of Carbamates and Nitrites with a Linear Transport Unit Connected to a Paper-Based Multiplexed Colorimetric Platform for Food Safety 与纸质多路比色平台连接的线性传输单元检测氨基甲酸盐和亚硝酸盐的食品安全
4区 医学 Q4 Medicine Pub Date : 2023-05-28 DOI: 10.37290/ctnr2641-452x.21:448-453
Sung-Yu Wu, Yu-Chien Hung, Kuan-Hung Chen, Connie Chen, Chao-Min Cheng, Min-Yen Hsu
The COVID-19 pandemic and its associated restrictions made the concept of home testing increasingly popular. Here, we designed a linear transport unit connected to a paper-based multiplexed colorimetric platform, which can be used to test for food safety and physiological status. The device is composed of a linear transport unit and a paper-based multiplexed colorimetric reaction unit. The linear transporting unit uses the capillary phenomenon to collect samples, and the multiplexed colorimetric reaction unit can be used to detect different targets using a hydrophilic area and wax as the hydrophobic barrier. A rapid and multiplexed naked-eye readout of the colorimetric response is achieved in a few minutes. In this study, we demonstrate that the device can be used to detect nitrite, pesticides, lactate, and glycogen. It is a convenient and cheap home self-testing device that can be applied to different situations. This device could lead to earlier screening and, consequently, earlier referrals for further confirmation and treatment.
COVID-19大流行及其相关限制使家庭检测的概念越来越受欢迎。在这里,我们设计了一个线性传输单元,连接到基于纸张的多路比色平台,可用于检测食品安全和生理状态。该装置由线性传输单元和基于纸张的多路比色反应单元组成。线性输送单元利用毛细现象采集样品,多路比色反应单元利用亲水区域和蜡作为疏水屏障,可用于检测不同的靶标。在几分钟内实现了比色响应的快速和多路裸眼读出。在这项研究中,我们证明了该装置可用于检测亚硝酸盐,农药,乳酸盐和糖原。它是一种方便、廉价的家庭自检装置,可以适用于不同的情况。该设备可以导致早期筛查,因此,早期转诊进一步确认和治疗。
{"title":"Detection of Carbamates and Nitrites with a Linear Transport Unit Connected to a Paper-Based Multiplexed Colorimetric Platform for Food Safety","authors":"Sung-Yu Wu, Yu-Chien Hung, Kuan-Hung Chen, Connie Chen, Chao-Min Cheng, Min-Yen Hsu","doi":"10.37290/ctnr2641-452x.21:448-453","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:448-453","url":null,"abstract":"The COVID-19 pandemic and its associated restrictions made the concept of home testing increasingly popular. Here, we designed a linear transport unit connected to a paper-based multiplexed colorimetric platform, which can be used to test for food safety and physiological status. The device is composed of a linear transport unit and a paper-based multiplexed colorimetric reaction unit. The linear transporting unit uses the capillary phenomenon to collect samples, and the multiplexed colorimetric reaction unit can be used to detect different targets using a hydrophilic area and wax as the hydrophobic barrier. A rapid and multiplexed naked-eye readout of the colorimetric response is achieved in a few minutes. In this study, we demonstrate that the device can be used to detect nitrite, pesticides, lactate, and glycogen. It is a convenient and cheap home self-testing device that can be applied to different situations. This device could lead to earlier screening and, consequently, earlier referrals for further confirmation and treatment.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135897627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimizing Biosynthesis of Nicotinamide Mononucleotide, a Potential Nutritional Supplement, by E. coli 大肠杆菌优化潜在营养补充剂烟酰胺单核苷酸的生物合成
IF 0.5 4区 医学 Q4 Medicine Pub Date : 2023-05-05 DOI: 10.37290/ctnr2641-452x.21:198-207
Yujuan Cao, Lun Cui
Nicotinamide mononucleotide plays a crucial role in the biosynthesis of the coenzyme nicotinamide adenine dinucleotide, which is involved in various metabolic pathways in all living organisms. Nicotinamide mononucleotide has recently received attention for its potential health benefits in aging, weight loss, diabetes, and as a neuroprotectant. In this study, we aimed to optimize the production of nicotinamide mononucleotide in E. coli with combinations of genes and plasmids. Our results show that nicotinamide mononucleotide production in Escherichia coli is highly related to bacterial growth rate, indicating that the highest nicotinamide mononucleotide production can be achieved by strains with moderate growth rates. This finding highlights the importance of maintaining a balanced growth rate to achieve optimal nicotinamide mononucleotide production in E. coli. Our study provides insights into optimizing the in vivo synthesis of nicotinamide mononucleotide, a potential agent for nutritional supplementation for novel therapies in various age-related diseases.
烟酰胺单核苷酸在辅酶烟酰胺腺嘌呤二核苷酸的生物合成中起着至关重要的作用,该二核苷酸参与所有生物体的各种代谢途径。烟酰胺单核苷酸最近因其在衰老、减肥、糖尿病和神经保护方面的潜在健康益处而受到关注。在本研究中,我们旨在通过基因和质粒的组合优化大肠杆菌中烟酰胺单核苷酸的生产。我们的研究结果表明,大肠杆菌中烟酰胺单核苷酸的产生与细菌生长速率高度相关,这表明中等生长速率的菌株可以实现最高的烟酰胺单核苷酸产量。这一发现强调了在大肠杆菌中保持平衡生长速率以实现最佳烟酰胺单核苷酸生产的重要性。我们的研究为优化烟酰胺单核苷酸的体内合成提供了见解,烟酰胺单核苷酸是一种潜在的营养补充剂,可用于各种年龄相关疾病的新疗法。
{"title":"Optimizing Biosynthesis of Nicotinamide Mononucleotide, a Potential Nutritional Supplement, by E. coli","authors":"Yujuan Cao, Lun Cui","doi":"10.37290/ctnr2641-452x.21:198-207","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:198-207","url":null,"abstract":"Nicotinamide mononucleotide plays a crucial role in the biosynthesis of the coenzyme nicotinamide adenine dinucleotide, which is involved in various metabolic pathways in all living organisms. Nicotinamide mononucleotide has recently received attention for its potential health benefits in aging, weight loss, diabetes, and as a neuroprotectant. In this study, we aimed to optimize the production of nicotinamide mononucleotide in E. coli with combinations of genes and plasmids. Our results show that nicotinamide mononucleotide production in Escherichia coli is highly related to bacterial growth rate, indicating that the highest nicotinamide mononucleotide production can be achieved by strains with moderate growth rates. This finding highlights the importance of maintaining a balanced growth rate to achieve optimal nicotinamide mononucleotide production in E. coli. Our study provides insights into optimizing the in vivo synthesis of nicotinamide mononucleotide, a potential agent for nutritional supplementation for novel therapies in various age-related diseases.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42327975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of Garlic (Allium Sativum L.) in Chronic Kidney Diseases: A Short Review 大蒜在慢性肾脏疾病中的作用
IF 0.5 4区 医学 Q4 Medicine Pub Date : 2023-05-02 DOI: 10.37290/ctnr2641-452x.21:1-4
Chien-Ming Lin, Huangfu Chao, Han-Fang Tseng, Chih-Hung Lin, C. Kuo
Chronic kidney disease is characterized by persistent abnormalities in urinary function, structural abnormalities, or impaired excretory functions that indicate the loss of functional nephrons. Cardiovascular disease and death are the most common complications of chronic kidney disease. Once chronic kidney disease is established, treatment has proven ineffective. Therefore, research efforts should be devoted to uncovering early disease mechanisms and alternative treatment options. Traditionally, garlic has been used as a pharmacological agent, especially when aged, in addition to its application as a food flavoring agent. Studies have suggested that garlic has anti-inflammatory and immunomodulatory properties. The effects of garlic on renal function have been summarized in cell culture, animal, and clinical studies.
慢性肾脏疾病的特征是尿功能持续异常、结构异常或排泄功能受损,表明肾单位功能丧失。心血管疾病和死亡是慢性肾脏疾病最常见的并发症。一旦慢性肾脏疾病确立,治疗已被证明是无效的。因此,研究工作应致力于揭示早期疾病机制和替代治疗方案。传统上,大蒜除了用作食品调味剂外,还被用作药理剂,尤其是在陈化时。研究表明,大蒜具有抗炎和免疫调节的特性。大蒜对肾功能的影响已在细胞培养、动物和临床研究中综述。
{"title":"Role of Garlic (Allium Sativum L.) in Chronic Kidney Diseases: A Short Review","authors":"Chien-Ming Lin, Huangfu Chao, Han-Fang Tseng, Chih-Hung Lin, C. Kuo","doi":"10.37290/ctnr2641-452x.21:1-4","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:1-4","url":null,"abstract":"Chronic kidney disease is characterized by persistent abnormalities in urinary function, structural abnormalities, or impaired excretory functions that indicate the loss of functional nephrons. Cardiovascular disease and death are the most common complications of chronic kidney disease. Once chronic kidney disease is established, treatment has proven ineffective. Therefore, research efforts should be devoted to uncovering early disease mechanisms and alternative treatment options. Traditionally, garlic has been used as a pharmacological agent, especially when aged, in addition to its application as a food flavoring agent. Studies have suggested that garlic has anti-inflammatory and immunomodulatory properties. The effects of garlic on renal function have been summarized in cell culture, animal, and clinical studies.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41660197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of Garlic (Allium Sativum L.) in Chronic Kidney Diseases: A Short Review 大蒜在慢性肾脏疾病中的作用综述
4区 医学 Q4 Medicine Pub Date : 2023-05-02 DOI: 10.37290/ctnr2641-452x.21:194-197
Chien-Ming Lin, Huan-Nung Chao, Han-Fang Tseng, Chih-Hung Lin, Chan-Yen Kuo
Chronic kidney disease is characterized by persistent abnormalities in urinary function, structural abnormalities, or impaired excretory functions that indicate the loss of functional nephrons. Cardiovascular disease and death are the most common complications of chronic kidney disease. Once chronic kidney disease is established, treatment has proven ineffective. Therefore, research efforts should be devoted to uncovering early disease mechanisms and alternative treatment options. Traditionally, garlic has been used as a pharmacological agent, especially when aged, in addition to its application as a food flavoring agent. Studies have suggested that garlic has anti-inflammatory and immunomodulatory properties. The effects of garlic on renal function have been summarized in cell culture, animal, and clinical studies.
慢性肾脏疾病的特征是尿功能持续异常、结构异常或排泄功能受损,这表明功能性肾单位的丧失。心血管疾病和死亡是慢性肾脏疾病最常见的并发症。一旦确定患有慢性肾病,治疗已被证明是无效的。因此,研究工作应致力于揭示早期疾病机制和替代治疗方案。传统上,大蒜除了作为食品调味剂外,还被用作药理学剂,尤其是在陈年后。研究表明,大蒜具有抗炎和免疫调节的特性。大蒜对肾功能的影响已经在细胞培养、动物和临床研究中得到了总结。
{"title":"Role of Garlic (Allium Sativum L.) in Chronic Kidney Diseases: A Short Review","authors":"Chien-Ming Lin, Huan-Nung Chao, Han-Fang Tseng, Chih-Hung Lin, Chan-Yen Kuo","doi":"10.37290/ctnr2641-452x.21:194-197","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:194-197","url":null,"abstract":"Chronic kidney disease is characterized by persistent abnormalities in urinary function, structural abnormalities, or impaired excretory functions that indicate the loss of functional nephrons. Cardiovascular disease and death are the most common complications of chronic kidney disease. Once chronic kidney disease is established, treatment has proven ineffective. Therefore, research efforts should be devoted to uncovering early disease mechanisms and alternative treatment options. Traditionally, garlic has been used as a pharmacological agent, especially when aged, in addition to its application as a food flavoring agent. Studies have suggested that garlic has anti-inflammatory and immunomodulatory properties. The effects of garlic on renal function have been summarized in cell culture, animal, and clinical studies.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135270253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Functional Assessment of Therapy Tools for Cancer Cachexia: A Review 癌症恶病质治疗工具的功能评价综述
IF 0.5 4区 医学 Q4 Medicine Pub Date : 2023-04-18 DOI: 10.37290/ctnr2641-452x.21:187-193
Lee Tang, John Jenn Yenn Lu, Sheau-Chung Tang
Cachexia is a multifactorial and multi-organ condition that increases the mortality rate of patients with advanced cancer. Amelioration of cancer patients’ weight loss and inflammatory status are critical to the treatment’s success. Cachexia contains a significant drop in body weight and a rapid loss of lipoprotein, accompanied by an inflammatory state. In the late stage of cancer, anorexia and cachexia may occur at the same time, a condition called anorexia and cachexia syndrome. Keeping abreast of the patient’s inflammatory and nutritional status is of utmost importance for optimal treatment. This review explores the contribution of nutrient changes and the novel biomarker proteins in anorexia and cachexia syndrome. Functional assessment using anorexia and cachexia therapy assessment tools can monitor and provide early signals for anorexia and cachexia syndrome and suggest mechanism-based interventions to improve the status of cachexia with appropriate nutrients or drugs.
恶病质是一种多因素、多器官的疾病,会增加晚期癌症患者的死亡率。癌症患者的体重减轻和炎症状况的改善是治疗成功的关键。恶病质包括体重的显著下降和脂蛋白的快速损失,并伴有炎症状态。在癌症晚期,厌食和恶病质可能同时发生,这种情况被称为厌食和恶病质综合征。及时了解患者的炎症和营养状况对最佳治疗至关重要。本文综述了营养变化和新的生物标志物蛋白在厌食症和恶病质综合征中的作用。使用厌食症和恶病质治疗评估工具进行功能评估可以监测并提供厌食症和恶病质综合征的早期信号,并建议基于机制的干预措施,通过适当的营养或药物来改善恶病质状况。
{"title":"Functional Assessment of Therapy Tools for Cancer Cachexia: A Review","authors":"Lee Tang, John Jenn Yenn Lu, Sheau-Chung Tang","doi":"10.37290/ctnr2641-452x.21:187-193","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:187-193","url":null,"abstract":"Cachexia is a multifactorial and multi-organ condition that increases the mortality rate of patients with advanced cancer. Amelioration of cancer patients’ weight loss and inflammatory status are critical to the treatment’s success. Cachexia contains a significant drop in body weight and a rapid loss of lipoprotein, accompanied by an inflammatory state. In the late stage of cancer, anorexia and cachexia may occur at the same time, a condition called anorexia and cachexia syndrome. Keeping abreast of the patient’s inflammatory and nutritional status is of utmost importance for optimal treatment. This review explores the contribution of nutrient changes and the novel biomarker proteins in anorexia and cachexia syndrome. Functional assessment using anorexia and cachexia therapy assessment tools can monitor and provide early signals for anorexia and cachexia syndrome and suggest mechanism-based interventions to improve the status of cachexia with appropriate nutrients or drugs.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47815752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Effects of Medium-Term Intake of Hydrogen-Rich Water on Sperm Quality Biomarkers in Normospermic and Oligospermic Men: A Randomized Controlled Pilot Trial 中期摄入富氢水对正常精子和少精子男性精子质量生物标志物的影响:一项随机对照试验
IF 0.5 4区 医学 Q4 Medicine Pub Date : 2023-04-11 DOI: 10.37290/ctnr2641-452x.21:182-186
V. Štajer, N. Todorovic, Branislava Drdjenovic Conic, N. Kladar, D. Engeset, T. H. Stea, L. Rátgéber, S. Forbes, D. Candow, S. Ostojić
The main aim of this randomized controlled pilot trial was to evaluate the effects of hydrogen-rich water on spermiogram parameters in normospermic and oligospermic men. As many as 12 healthy young men (age 29.1 ± 5.9 years; n = 6 normospermic; n = 6 oligospermic) volunteered. Participants were allocated in a double-blind manner to receive 1 L of hydrogen-rich water per day or 1 L of tap water fortified with magnesium for 8 weeks. Following hydrogen-rich water supplementation, sperm concentration and morphology tended to increase by 12.4 million per milliliter (95% CI; –31.8 to 56.6), and live cells increased by 3.8% (95% CI; –12.5 to 20.1), respectively (P ≤ 0.30). A significant difference between the two interventions was found in sperm vitality (P = 0.03), with hydrogen-rich water being superior to the placebo in terms of an increase in the number of live sperm cells. Subgroup analyses revealed no significant differences between interventions in sperm viability outcomes in oligospermic men (P > 0.05). However, sperm count increased by over 20 million in two of the three oligospermic men (66.7%) receiving hydrogen-rich water, while no oligospermic men in the placebo group increased sperm concentration to euspermic levels (P < 0.01). These preliminary findings suggest hydrogen-rich water is an effective intervention for improving some aspects of male subfertility; further large-sample trials are warranted to corroborate our results.
这项随机对照先导试验的主要目的是评估富氢水对正常精子和少精子男性精子图参数的影响。健康青年男性12人(年龄29.1±5.9岁;N = 6;N = 6,少精子)自愿。参与者以双盲方式被分配,每天接受1升富氢水或1升加镁的自来水,持续8周。补充富氢水后,精子浓度和形态倾向于每毫升增加1240万个(95% CI;-31.8至56.6),活细胞增加3.8% (95% CI;-12.5 ~ 20.1) (P≤0.30)。两种干预措施在精子活力方面存在显著差异(P = 0.03),富氢水在活精子细胞数量增加方面优于安慰剂。亚组分析显示,不同干预措施对少精子男性精子生存能力的影响无显著差异(P < 0.05)。然而,在接受富氢水治疗的三名少精子男性中,有两名(66.7%)精子数量增加了2000多万,而安慰剂组的少精子男性精子浓度没有增加到原精子水平(P < 0.01)。这些初步研究结果表明,富氢水是改善男性不育某些方面的有效干预措施;需要进一步的大样本试验来证实我们的结果。
{"title":"The Effects of Medium-Term Intake of Hydrogen-Rich Water on Sperm Quality Biomarkers in Normospermic and Oligospermic Men: A Randomized Controlled Pilot Trial","authors":"V. Štajer, N. Todorovic, Branislava Drdjenovic Conic, N. Kladar, D. Engeset, T. H. Stea, L. Rátgéber, S. Forbes, D. Candow, S. Ostojić","doi":"10.37290/ctnr2641-452x.21:182-186","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:182-186","url":null,"abstract":"The main aim of this randomized controlled pilot trial was to evaluate the effects of hydrogen-rich water on spermiogram parameters in normospermic and oligospermic men. As many as 12 healthy young men (age 29.1 ± 5.9 years; n = 6 normospermic; n = 6 oligospermic) volunteered. Participants were allocated in a double-blind manner to receive 1 L of hydrogen-rich water per day or 1 L of tap water fortified with magnesium for 8 weeks. Following hydrogen-rich water supplementation, sperm concentration and morphology tended to increase by 12.4 million per milliliter (95% CI; –31.8 to 56.6), and live cells increased by 3.8% (95% CI; –12.5 to 20.1), respectively (P ≤ 0.30). A significant difference between the two interventions was found in sperm vitality (P = 0.03), with hydrogen-rich water being superior to the placebo in terms of an increase in the number of live sperm cells. Subgroup analyses revealed no significant differences between interventions in sperm viability outcomes in oligospermic men (P > 0.05). However, sperm count increased by over 20 million in two of the three oligospermic men (66.7%) receiving hydrogen-rich water, while no oligospermic men in the placebo group increased sperm concentration to euspermic levels (P < 0.01). These preliminary findings suggest hydrogen-rich water is an effective intervention for improving some aspects of male subfertility; further large-sample trials are warranted to corroborate our results.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42593532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plantamajoside Reduces Gastric Cancer Cell Migration and Invasion by Inhibiting PI3K/AKT and ERK Pathways 车前草皂苷通过抑制PI3K/AKT和ERK通路减少胃癌细胞的迁移和侵袭
IF 0.5 4区 医学 Q4 Medicine Pub Date : 2023-04-11 DOI: 10.37290/ctnr2641-452x.21:175-181
Cheng-fang Tang, Yuanyuan Wang, Jing Huang, Xiaohong Lv
Gastric cancer is a common malignancy with a poor survival rate. Plantamajoside, a phenylethanoid glycoside from Plantago asiatica, has been shown to inhibit the progression of many different types of cancer; however, its effect on gastric cancer remains to be explored. To this end, the effect of plantamajoside on the proliferation, apoptosis, stemness, mobility, and invasion of gastric cancer was evaluated by cell survival assay, flow cytometry, colony formation, sphere formation, transwell, and Western blot assays. Also, the potential molecular mechanism was addressed by Western blot assays for the expression of various signal proteins. Plantamajoside reduced cell viability, enhanced apoptosis, reduced cell mobility, and increased invasion of both AGS and HS746T cells in a dose-dependent manner. Plantamajoside also decreased the diameter of the spheroid and the relative expression of octamer-binding transcription factor-4 and the cluster of differentiation 44 proteins in both AGS and HS746T cells. Furthermore, plantamajoside downregulated the relative protein levels of p-PI3K/ PI3K, p-AKT/AKT, and p-ERK/ERK in both AGS and HS746T cells.
胃癌是一种常见的恶性肿瘤,生存率很低。车前草皂苷是一种来自车前草的苯乙醇苷,已被证明可以抑制许多不同类型癌症的进展;然而,其对胃癌的影响仍有待探讨。为此,采用细胞存活试验、流式细胞术、集落形成、球形成、transwell、Western blot等方法,评价了金跖皂苷对胃癌细胞增殖、凋亡、干性、移动性和侵袭性的影响。此外,通过Western blot检测各种信号蛋白的表达,探讨了潜在的分子机制。车前草皂苷降低细胞活力,增强细胞凋亡,降低细胞流动性,增加AGS和HS746T细胞的侵袭,并呈剂量依赖性。车前草皂苷还能降低AGS和HS746T细胞的球体直径、八聚体结合转录因子-4和分化44蛋白簇的相对表达量。此外,车前草皂苷下调AGS和HS746T细胞中p-PI3K/ PI3K、p-AKT/AKT和p-ERK/ERK的相对蛋白水平。
{"title":"Plantamajoside Reduces Gastric Cancer Cell Migration and Invasion by Inhibiting PI3K/AKT and ERK Pathways","authors":"Cheng-fang Tang, Yuanyuan Wang, Jing Huang, Xiaohong Lv","doi":"10.37290/ctnr2641-452x.21:175-181","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:175-181","url":null,"abstract":"Gastric cancer is a common malignancy with a poor survival rate. Plantamajoside, a phenylethanoid glycoside from Plantago asiatica, has been shown to inhibit the progression of many different types of cancer; however, its effect on gastric cancer remains to be explored. To this end, the effect of plantamajoside on the proliferation, apoptosis, stemness, mobility, and invasion of gastric cancer was evaluated by cell survival assay, flow cytometry, colony formation, sphere formation, transwell, and Western blot assays. Also, the potential molecular mechanism was addressed by Western blot assays for the expression of various signal proteins. Plantamajoside reduced cell viability, enhanced apoptosis, reduced cell mobility, and increased invasion of both AGS and HS746T cells in a dose-dependent manner. Plantamajoside also decreased the diameter of the spheroid and the relative expression of octamer-binding transcription factor-4 and the cluster of differentiation 44 proteins in both AGS and HS746T cells. Furthermore, plantamajoside downregulated the relative protein levels of p-PI3K/ PI3K, p-AKT/AKT, and p-ERK/ERK in both AGS and HS746T cells.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42551756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protection of Neuronal Cells from Lipopolysaccharide-Induced Systemic Inflammation by Gossypetin 棉酚素对神经元细胞的保护作用
IF 0.5 4区 医学 Q4 Medicine Pub Date : 2023-04-07 DOI: 10.37290/ctnr2641-452x.21:138-143
Soyoung Jang, Soyeon Jang, Jiwon Ko, Eungyung Kim, Hyejin Hyung, Ji Yeong Park, Su-Geun Lim, S. Park, M. Kim, Z. Ryoo
Systemic inflammation caused by infection, surgery, or injury can lead to cognitive decline. Lipopolysaccharides are known as toll-like receptor 4 ligands, which are common to the cell walls of gram-negative bacteria. Activation of toll-like receptor 4 leads to the production of proinflammatory cytokines that subsequently mediate systemic inflammation. Furthermore, induc¬tion of systemic inflammation by lipopolysaccharide injection in mice can affect the brain, including cognitive functions. To investigate the neuroprotective role of gossypetin in systemic inflammation, a mouse hippocampal cell line (HT22) and mice were challenged with lipopolysaccharide. The increase in proinflammatory cytokines and reactive oxygen species caused by lipopolysaccharide treatment in HT22 cells was decreased by gossypetin treatment. To evaluate the protective function against memory impairment, gossypetin was orally administered to C57BL/6J mice receiving lipopolysaccharide injec¬tion. Lipopolysaccharide-induced memory deficit was observed in lipopolysaccharide-only treated group in Y-maze test. However, the group treated with gossypetin and lipopolysaccharide had a diminution in cognitive impairment. Consistent with the behavioral test results, the proinflammatory cytokines were also relatively downregulated in the gossypetin-treated mouse group. To sum up, gossypetin can be protect the neuron cells from inflammation in vitro and prevent the cognitive impairment in mice in vivo.
由感染、手术或损伤引起的全身炎症可导致认知能力下降。脂多糖被称为toll样受体4配体,常见于革兰氏阴性菌的细胞壁。toll样受体4的激活导致促炎细胞因子的产生,随后介导全身炎症。此外,脂多糖注射诱导小鼠全身炎症会影响大脑,包括认知功能。为了研究棉子素在全身炎症中的神经保护作用,用脂多糖攻击小鼠海马细胞系(HT22)和小鼠。棉子素处理减少了脂多糖处理引起的HT22细胞中促炎细胞因子和活性氧的增加。为了评估对记忆损伤的保护作用,对接受脂多糖注射的C57BL/6J小鼠口服棉子素。在Y迷宫试验中,仅脂多糖处理组观察到脂多糖诱导的记忆缺陷。然而,棉子素和脂多糖治疗组的认知障碍有所减轻。与行为测试结果一致,在棉子素治疗的小鼠组中,促炎细胞因子也相对下调。综上所述,棉子素在体外具有保护神经元细胞免受炎症的作用,在体内具有预防小鼠认知功能障碍的作用。
{"title":"Protection of Neuronal Cells from Lipopolysaccharide-Induced Systemic Inflammation by Gossypetin","authors":"Soyoung Jang, Soyeon Jang, Jiwon Ko, Eungyung Kim, Hyejin Hyung, Ji Yeong Park, Su-Geun Lim, S. Park, M. Kim, Z. Ryoo","doi":"10.37290/ctnr2641-452x.21:138-143","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:138-143","url":null,"abstract":"Systemic inflammation caused by infection, surgery, or injury can lead to cognitive decline. Lipopolysaccharides are known as toll-like receptor 4 ligands, which are common to the cell walls of gram-negative bacteria. Activation of toll-like receptor 4 leads to the production of proinflammatory cytokines that subsequently mediate systemic inflammation. Furthermore, induc¬tion of systemic inflammation by lipopolysaccharide injection in mice can affect the brain, including cognitive functions. To investigate the neuroprotective role of gossypetin in systemic inflammation, a mouse hippocampal cell line (HT22) and mice were challenged with lipopolysaccharide. The increase in proinflammatory cytokines and reactive oxygen species caused by lipopolysaccharide treatment in HT22 cells was decreased by gossypetin treatment. To evaluate the protective function against memory impairment, gossypetin was orally administered to C57BL/6J mice receiving lipopolysaccharide injec¬tion. Lipopolysaccharide-induced memory deficit was observed in lipopolysaccharide-only treated group in Y-maze test. However, the group treated with gossypetin and lipopolysaccharide had a diminution in cognitive impairment. Consistent with the behavioral test results, the proinflammatory cytokines were also relatively downregulated in the gossypetin-treated mouse group. To sum up, gossypetin can be protect the neuron cells from inflammation in vitro and prevent the cognitive impairment in mice in vivo.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47818170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Physalin A Enhances Autophagy and Suppresses Oxidative Stress Injury and Extracellular Matrix Deposition in Diabetic Nephropathy Physalin A促进糖尿病肾病的自噬,抑制氧化应激损伤和细胞外基质沉积
IF 0.5 4区 医学 Q4 Medicine Pub Date : 2023-04-07 DOI: 10.37290/ctnr2641-452x.21:131-137
Miao Zhang, Dan-dan Huang, Dan Chen, Taotao Hu
Diabetic nephropathy is the major cause of chronic kidney disease worldwide. Persistent hyperglycemia plays a causative role in diabetic nephropathy. Physalin A, a steroidal plant-derived bioactive compound known to exhibit anti-inflammatory, anti-tumor, anti-fungal, and analgesic activities, has been widely used in traditional Chinese medicine practice. The present study examined the effect of Physalin A on high-glucose-treated HBZY-1 cells, a cell culture model of diabetic nephropathy. The results show that Physalin A alleviated cell proliferation, oxidative stress, extracellular matrix protein deposition, and insufficient autophagy induced by high glucose through inhibiting the mitogen-activated protein kinase pathway.
糖尿病肾病是世界范围内慢性肾脏疾病的主要病因。持续性高血糖在糖尿病肾病中起致病作用。Physalin A是一种甾体植物衍生的生物活性化合物,具有抗炎、抗肿瘤、抗真菌和镇痛活性,已广泛应用于中药实践。本研究检测了藻盐A对高糖处理的糖尿病肾病细胞培养模型HBZY-1细胞的影响。结果表明,藻盐蛋白A通过抑制丝裂原活化蛋白激酶途径,减轻高糖诱导的细胞增殖、氧化应激、细胞外基质蛋白沉积和自噬不足。
{"title":"Physalin A Enhances Autophagy and Suppresses Oxidative Stress Injury and Extracellular Matrix Deposition in Diabetic Nephropathy","authors":"Miao Zhang, Dan-dan Huang, Dan Chen, Taotao Hu","doi":"10.37290/ctnr2641-452x.21:131-137","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:131-137","url":null,"abstract":"Diabetic nephropathy is the major cause of chronic kidney disease worldwide. Persistent hyperglycemia plays a causative role in diabetic nephropathy. Physalin A, a steroidal plant-derived bioactive compound known to exhibit anti-inflammatory, anti-tumor, anti-fungal, and analgesic activities, has been widely used in traditional Chinese medicine practice. The present study examined the effect of Physalin A on high-glucose-treated HBZY-1 cells, a cell culture model of diabetic nephropathy. The results show that Physalin A alleviated cell proliferation, oxidative stress, extracellular matrix protein deposition, and insufficient autophagy induced by high glucose through inhibiting the mitogen-activated protein kinase pathway.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41873268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Huangqi Granules Combined with Budesonide on Clinical Indexes, Clinical Efficacy, and Safety in Children with Bronchial Asthma 黄芪颗粒联合布地奈德对儿童支气管哮喘临床指标、疗效及安全性的影响
IF 0.5 4区 医学 Q4 Medicine Pub Date : 2023-04-07 DOI: 10.37290/ctnr2641-452x.21:169-174
Fuzhe Chen, Lei Liang, Changlong Lu, Chongyu Xu
We have investigated the effect of a combination of Budesonide and Huangqi granules on pulmonary function in children with bronchial asthma. To this end, children diagnosed with bronchial asthma were divided into two treatment groups, one receiving Budesonide alone (the control group) and the other receiving additional Huangqi granules (the study group). The syndrome score, pulmonary and immune function indices, clinical efficacy, and adverse reactions were observed and compared between the two groups. Before treatment, there was no significant difference in any of the measures between the two groups. However, after treatment, all measures were significantly improved in both groups, with a greater improvement in the study group. Also, the difference in the adverse reaction rate between the two groups was insignificant. In conclusion, Huangqi granules combined with Budesonide can effectively improve the clinical symptoms and repair pulmonary function in children with bronchial asthma.
我们研究了布地奈德联合黄芪颗粒对支气管哮喘患儿肺功能的影响。为此,将诊断为支气管哮喘的儿童分为两组,一组单独使用布地奈德(对照组),另一组使用黄芪颗粒(研究组)。观察比较两组患者的证候评分、肺功能、免疫功能指标、临床疗效及不良反应。治疗前,两组间各项指标均无显著差异。然而,治疗后,两组的各项指标均有显著改善,研究组改善更大。两组不良反应发生率差异无统计学意义。综上所述,黄芪颗粒联合布地奈德可有效改善支气管哮喘患儿的临床症状,修复肺功能。
{"title":"Effect of Huangqi Granules Combined with Budesonide on Clinical Indexes, Clinical Efficacy, and Safety in Children with Bronchial Asthma","authors":"Fuzhe Chen, Lei Liang, Changlong Lu, Chongyu Xu","doi":"10.37290/ctnr2641-452x.21:169-174","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:169-174","url":null,"abstract":"We have investigated the effect of a combination of Budesonide and Huangqi granules on pulmonary function in children with bronchial asthma. To this end, children diagnosed with bronchial asthma were divided into two treatment groups, one receiving Budesonide alone (the control group) and the other receiving additional Huangqi granules (the study group). The syndrome score, pulmonary and immune function indices, clinical efficacy, and adverse reactions were observed and compared between the two groups. Before treatment, there was no significant difference in any of the measures between the two groups. However, after treatment, all measures were significantly improved in both groups, with a greater improvement in the study group. Also, the difference in the adverse reaction rate between the two groups was insignificant. In conclusion, Huangqi granules combined with Budesonide can effectively improve the clinical symptoms and repair pulmonary function in children with bronchial asthma.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47101632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Current Topics in Nutraceutical Research
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1