Pub Date : 2023-04-07DOI: 10.37290/ctnr2641-452x.21:144-149
Mingyue Shen, M. Wu, Jing Gu
We have explored the effect of nutrition management based on the principles of traditional Chinese medicine nutrition on the complications of subclinical gestational hypothyroidism. A total of 228 pregnant women from the Affiliated Hospital of Jiangnan University and the Wuxi Xishan People’s Hospital who were diagnosed with subclinical hypothyroidism between June 2020 and April 2022 were recruited. The patients were divided into the control group (n = 120) and intervention group (n = 108) using the historical control method. The intervention group adopted the traditional Chinese medicine nutrition management model, while the control group was given conventional gestational nutrition management. Iodine nutritional status, maternal and neonatal outcomes, pregnancy complications, pregnancy stress, and social support were compared between the two groups before intervention, at 20 weeks of pregnancy, and before labor. The intervention group had significantly lower iodine nutritional status, better postpartum nutritional status, better pregnancy outcome, lower incidences of pregnancy complications, and better pregnancy stress and social support than in the control group (P < 0.05). In conclusion, traditional Chinese medicine-based nutrition management can effectively control iodine nutritional status, improve maternal and neonatal outcomes, reduce pregnancy complications, and decrease pregnancy stress in postpartum women.
{"title":"Nutrition Based on the Traditional Chinese Medicine and Complications of Subclinical Gestational Hypothyroidism","authors":"Mingyue Shen, M. Wu, Jing Gu","doi":"10.37290/ctnr2641-452x.21:144-149","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:144-149","url":null,"abstract":"We have explored the effect of nutrition management based on the principles of traditional Chinese medicine nutrition on the complications of subclinical gestational hypothyroidism. A total of 228 pregnant women from the Affiliated Hospital of Jiangnan University and the Wuxi Xishan People’s Hospital who were diagnosed with subclinical hypothyroidism between June 2020 and April 2022 were recruited. The patients were divided into the control group (n = 120) and intervention group (n = 108) using the historical control method. The intervention group adopted the traditional Chinese medicine nutrition management model, while the control group was given conventional gestational nutrition management. Iodine nutritional status, maternal and neonatal outcomes, pregnancy complications, pregnancy stress, and social support were compared between the two groups before intervention, at 20 weeks of pregnancy, and before labor. The intervention group had significantly lower iodine nutritional status, better postpartum nutritional status, better pregnancy outcome, lower incidences of pregnancy complications, and better pregnancy stress and social support than in the control group (P < 0.05). In conclusion, traditional Chinese medicine-based nutrition management can effectively control iodine nutritional status, improve maternal and neonatal outcomes, reduce pregnancy complications, and decrease pregnancy stress in postpartum women.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46365947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-07DOI: 10.37290/ctnr2641-452x.21:150-158
Xiaojing Li, Ning Li, Xiaoyi Yang, Cheng Zeng
Tilianin is a flavonoid glycoside with potential applications in the prevention and treatment of myocardial infarction, but its specific targets and pharmacological mechanisms are unclear. Therefore, in this study, a reverse screening and molecular docking approach was used to predict the pharmacological targets and mechanisms of tilianin on myocardial infarction based on network pharmacology and experimental validation. Firstly, we screened various databases to obtain potential core targets of tilianin. Secondly, we used protein–protein interaction, gene ontology, Kyoto Encyclopedia of Genes and Genomes, and ClueGO to correlate the key targets before molecular docking of new target genes. Finally, the gene expression of tilianin in HL-1 cells was experimentally validated with quantitative real-time polymerase chain reaction and immunofluorescence. The result showed that tilianin may significantly modulate pro-inflammatory effects in the tumor necrosis factor-α signaling pathway, which activates and regulates various cellular activities. Molecular docking revealed that protein kinase B1, proto-oncogene cellular sarcoma, tumor necrosis factor-α, matrix metalloproteinase-9, nitric oxide synthetase 2, mitogen-activated protein kinase 14 and phosphatase and tensin homolog-induced kinase 1 may be the key targets for the systematic regulatory effect of tilianin against myocardial infarction. Finally, quantitative real-time polymerase chain reaction and immunofluorescence results showed that tilianin significantly influences the expression of the key genes in myocardial infarction. Using network bioinformatic analysis, molecular docking techniques, and experimental validation, we predicted and preliminarily validated potential target sites for tilianin. These findings provide a new perspective for further exploration of the mechanism of action of tilianin in myocardial infarction therapy. Meanwhile, this study provides an objective basis for further experimental studies in the future.
{"title":"Network Pharmacology-Based Prediction and Experimental Validation of the Targets and Mechanism of Tilianin Action Against Myocardial Infarction","authors":"Xiaojing Li, Ning Li, Xiaoyi Yang, Cheng Zeng","doi":"10.37290/ctnr2641-452x.21:150-158","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:150-158","url":null,"abstract":"Tilianin is a flavonoid glycoside with potential applications in the prevention and treatment of myocardial infarction, but its specific targets and pharmacological mechanisms are unclear. Therefore, in this study, a reverse screening and molecular docking approach was used to predict the pharmacological targets and mechanisms of tilianin on myocardial infarction based on network pharmacology and experimental validation. Firstly, we screened various databases to obtain potential core targets of tilianin. Secondly, we used protein–protein interaction, gene ontology, Kyoto Encyclopedia of Genes and Genomes, and ClueGO to correlate the key targets before molecular docking of new target genes. Finally, the gene expression of tilianin in HL-1 cells was experimentally validated with quantitative real-time polymerase chain reaction and immunofluorescence. The result showed that tilianin may significantly modulate pro-inflammatory effects in the tumor necrosis factor-α signaling pathway, which activates and regulates various cellular activities. Molecular docking revealed that protein kinase B1, proto-oncogene cellular sarcoma, tumor necrosis factor-α, matrix metalloproteinase-9, nitric oxide synthetase 2, mitogen-activated protein kinase 14 and phosphatase and tensin homolog-induced kinase 1 may be the key targets for the systematic regulatory effect of tilianin against myocardial infarction. Finally, quantitative real-time polymerase chain reaction and immunofluorescence results showed that tilianin significantly influences the expression of the key genes in myocardial infarction. Using network bioinformatic analysis, molecular docking techniques, and experimental validation, we predicted and preliminarily validated potential target sites for tilianin. These findings provide a new perspective for further exploration of the mechanism of action of tilianin in myocardial infarction therapy. Meanwhile, this study provides an objective basis for further experimental studies in the future.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47355126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-07DOI: 10.37290/ctnr2641-452x.21:125-130
Hongyan Cai, X. Si
The relief from clinical symptoms of Helicobacter pylori infection caused by Lactobacillus acidophilus treatment was analyzed by a 13C-urea breath test. To this end, 200 patients diagnosed with Helicobacter pylori infection were divided into two equal groups of 100 patients, designated as the control and study groups, respectively. The control group received conventional quadruple therapy that included enteric-coated esomeprazole magnesium tablets, amoxicillin, clarithromycin, and colloidal pectin bismuth for 2 weeks. The study group, in addition to the conventional quadruple therapy, received Lactobacillus acidophilus tablets orally for 2 weeks. The Helicobacter pylori eradication rate (P < 0.05) and total effective rate (P < 0.05) of the study group were higher than the control group. The incidence of adverse reactions including acid reflux, upper abdom¬inal pain, abdominal fullness, and vomiting in the study group was lower (P < 0.05) than the control group. There was no statistically significant difference (P > 0.05) in the levels of tumor nuclear factor-α, high sensitivity C-reaction protein, and interlukin-6 between two groups before treatment. However, the level of these indicators in the study group after treatment was lower than that in the control group. Similarly, there was no statistically significant difference in CD3+, CD4+, CD8+, or CD4+/CD8+ between the two groups before treatment. However, the level of CD3+, CD4+, and CD4+/CD8+ after treatment was significantly higher, while CD8+ was significantly lower in the study group. In conclusion, Lactobacillus acidophilus treat¬ment exhibits a high eradication rate of Helicobacter pylori with good safety and relief from Helicobacter pylori-associated clinical symptoms.
{"title":"Lactobacillus acidophilus Enhances Relief from Clinical Symptoms of Helicobacter pylori Infection in Patients on Quadruple Therapy","authors":"Hongyan Cai, X. Si","doi":"10.37290/ctnr2641-452x.21:125-130","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:125-130","url":null,"abstract":"The relief from clinical symptoms of Helicobacter pylori infection caused by Lactobacillus acidophilus treatment was analyzed by a 13C-urea breath test. To this end, 200 patients diagnosed with Helicobacter pylori infection were divided into two equal groups of 100 patients, designated as the control and study groups, respectively. The control group received conventional quadruple therapy that included enteric-coated esomeprazole magnesium tablets, amoxicillin, clarithromycin, and colloidal pectin bismuth for 2 weeks. The study group, in addition to the conventional quadruple therapy, received Lactobacillus acidophilus tablets orally for 2 weeks. The Helicobacter pylori eradication rate (P < 0.05) and total effective rate (P < 0.05) of the study group were higher than the control group. The incidence of adverse reactions including acid reflux, upper abdom¬inal pain, abdominal fullness, and vomiting in the study group was lower (P < 0.05) than the control group. There was no statistically significant difference (P > 0.05) in the levels of tumor nuclear factor-α, high sensitivity C-reaction protein, and interlukin-6 between two groups before treatment. However, the level of these indicators in the study group after treatment was lower than that in the control group. Similarly, there was no statistically significant difference in CD3+, CD4+, CD8+, or CD4+/CD8+ between the two groups before treatment. However, the level of CD3+, CD4+, and CD4+/CD8+ after treatment was significantly higher, while CD8+ was significantly lower in the study group. In conclusion, Lactobacillus acidophilus treat¬ment exhibits a high eradication rate of Helicobacter pylori with good safety and relief from Helicobacter pylori-associated clinical symptoms.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46112294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-07DOI: 10.37290/ctnr2641-452x.21:123-124
Huangfu Chao, Han-Fang Tseng, Ya-Hsuan Lin, C. Kuo
In this short letter to the editor, we have introduced and summarized the role of anti-angiogenesis, microRNA, and exosomes in cancer treatment strategies.
在这封致编辑的短文中，我们介绍并总结了抗血管生成、microRNA和外泌体在癌症治疗策略中的作用。
{"title":"Strategies for Cancer Treatment: Anti-Angiogenesis, Exosomes, and micro-RNAs","authors":"Huangfu Chao, Han-Fang Tseng, Ya-Hsuan Lin, C. Kuo","doi":"10.37290/ctnr2641-452x.21:123-124","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:123-124","url":null,"abstract":"In this short letter to the editor, we have introduced and summarized the role of anti-angiogenesis, microRNA, and exosomes in cancer treatment strategies.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45974468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We have examined the influence of sequential enteral and parenteral nutrition support, assisted by nutritional risk screening, on colorectal cancer patients undergoing radical surgery. This cohort included 100 colorectal cancer patients receiving radical surgery in Zhejiang Quzhou People’s Hospital between May 2021 and April 2022. The control group received treat¬ment with early enteral nutrition support only. The experimental group, however, received sequential enteral and parenteral nutrition support, assisted by nutritional risk screening. The indicators of nutrition adequacy, gastrointestinal function, early postoperative complications, and quality of life were compared. The albumin, hemoglobin, and albumin levels presented no difference between the two groups before treatment. After treatment, these indicators in the experimental group were elevated relative to those in the control group. The time of bowel sound recovery, the time of first anal exhaustion, time of first defecation, and the incidence of surgical complications in the experimental group decreased relative to those in the control group. A 36-item short-form health survey showed an elevation in the experimental group relative to the control group. Application of sequential enteral and parenteral nutritional support assisted by nutritional risk screening in colorectal cancer patients has a significant effect, which helps improve postoperative nutritional status, facilitate gastrointestinal function recovery, reduce incidence of complications, and accelerate postoperative recovery.
{"title":"Sequential Enteral and Parenteral Nutrition Support Assisted by Nutritional Risk Screening Improves Outcome in the Colorectal Cancer Patients Undergoing Radical Surgery","authors":"Jing Gao, Tiansheng Xu, Li’an Xu, C-W Mao, Yujin Feng","doi":"10.37290/ctnr2641-452x.21:159-162","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:159-162","url":null,"abstract":"We have examined the influence of sequential enteral and parenteral nutrition support, assisted by nutritional risk screening, on colorectal cancer patients undergoing radical surgery. This cohort included 100 colorectal cancer patients receiving radical surgery in Zhejiang Quzhou People’s Hospital between May 2021 and April 2022. The control group received treat¬ment with early enteral nutrition support only. The experimental group, however, received sequential enteral and parenteral nutrition support, assisted by nutritional risk screening. The indicators of nutrition adequacy, gastrointestinal function, early postoperative complications, and quality of life were compared. The albumin, hemoglobin, and albumin levels presented no difference between the two groups before treatment. After treatment, these indicators in the experimental group were elevated relative to those in the control group. The time of bowel sound recovery, the time of first anal exhaustion, time of first defecation, and the incidence of surgical complications in the experimental group decreased relative to those in the control group. A 36-item short-form health survey showed an elevation in the experimental group relative to the control group. Application of sequential enteral and parenteral nutritional support assisted by nutritional risk screening in colorectal cancer patients has a significant effect, which helps improve postoperative nutritional status, facilitate gastrointestinal function recovery, reduce incidence of complications, and accelerate postoperative recovery.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49312203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-30DOI: 10.37290/ctnr2641-452x.21:495-501
Chu-yan Wang, Dong-sheng Wang, Jie Wu, Xia-wei Yin, Ze-hui Wang, Ye-xian Hu, Yan Zhang, Rong-an Zhu, Hong Mei
We have examined the basic composition of anthocyanins and polysaccharides, the active components in Aronia melanocarpa fruit. To this end, microwave-assisted ethanol extraction and cellulase enzymatic hydrolysis were employed to extract anthocyanins and crude polysaccharides, respectively. Aronia melanocarpa fruit polysaccharides were separated and purified by DEAE-52 cellulose and Sephadex G-100 glucan gel column chromatography, followed by high-performance liquid chromatography. The results demonstrated that the optimal extraction parameters of anthocyanin from Aronia melanocarpa fruit were a 60% ethanol volume fraction, a liquid-to-solid ratio of 30:1, a microwave time of 60 s, and a microwave power of 210 W. The extracted anthocyanins reached 200.47 mg/100 g, while the yield of polysaccharides was 3.82%. Anthocyanins in Aronia melanocarpa fruit were mainly composed of cyanidin, delphinidin, and mallow pigment, among which cyanidin was the main component, with a content as high as 90.79%. There were two distinct fractions of polysaccharides. The fraction-I polysaccharides were mainly composed of rhamnose, galacturonic acid, glucose, galactose, and arabinose, with a molar ratio of 8.80: 8.97: 2.38: 27.46: 47.94. In contrast, the fraction-II polysaccharides were primarily composed of rhamnose, glucuronic acid, galactose, xylose, and arabinose, with a molar ratio of 1.67: 9.22: 8.47: 70.69: 6.16. The results of this study can provide a reference for the in-depth development of Aronia melanocarpa resources
{"title":"Extraction and Compositional Analysis of Anthocyanins and Polysaccharides from Aronia Melanocarpa Fruit","authors":"Chu-yan Wang, Dong-sheng Wang, Jie Wu, Xia-wei Yin, Ze-hui Wang, Ye-xian Hu, Yan Zhang, Rong-an Zhu, Hong Mei","doi":"10.37290/ctnr2641-452x.21:495-501","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:495-501","url":null,"abstract":"We have examined the basic composition of anthocyanins and polysaccharides, the active components in Aronia melanocarpa fruit. To this end, microwave-assisted ethanol extraction and cellulase enzymatic hydrolysis were employed to extract anthocyanins and crude polysaccharides, respectively. Aronia melanocarpa fruit polysaccharides were separated and purified by DEAE-52 cellulose and Sephadex G-100 glucan gel column chromatography, followed by high-performance liquid chromatography. The results demonstrated that the optimal extraction parameters of anthocyanin from Aronia melanocarpa fruit were a 60% ethanol volume fraction, a liquid-to-solid ratio of 30:1, a microwave time of 60 s, and a microwave power of 210 W. The extracted anthocyanins reached 200.47 mg/100 g, while the yield of polysaccharides was 3.82%. Anthocyanins in Aronia melanocarpa fruit were mainly composed of cyanidin, delphinidin, and mallow pigment, among which cyanidin was the main component, with a content as high as 90.79%. There were two distinct fractions of polysaccharides. The fraction-I polysaccharides were mainly composed of rhamnose, galacturonic acid, glucose, galactose, and arabinose, with a molar ratio of 8.80: 8.97: 2.38: 27.46: 47.94. In contrast, the fraction-II polysaccharides were primarily composed of rhamnose, glucuronic acid, galactose, xylose, and arabinose, with a molar ratio of 1.67: 9.22: 8.47: 70.69: 6.16. The results of this study can provide a reference for the in-depth development of Aronia melanocarpa resources","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":"170 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135469249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-11DOI: 10.37290/ctnr2641-452x.21:102-108
R. Alqurashi, Mariam Alholaibi
This study investigated the effects of Moringa oleifera leaf, mulberry fruit, and mulberry leaf on high-fat diet-induced changes in body weight and hyperlipidemia in rats. Healthy male rats were randomly divided into five equal-sized groups that included (i) basal diet or negative control group, (ii) high-fat diet group (basal diet + 20% fat), (iii) M. oleifera leaf-treated high-fat diet group (high-fat diet + 10% M. oleifera leaf), (iv) mulberry fruit-treated high-fat diet group (high-fat diet + 10% mulberry fruit), and (v) mulberry leaf-treated high-fat diet group (high-fat diet + 10% mulberry leaf). The rats were treated daily for 6 weeks, and body weight data and blood samples were collected for each group. At the end of the study period, body weight, total cholesterol, total triglyceride, low-density lipoprotein level, and glucose level were significantly reduced in the rats fed a high-fat diet supplemented with M. oleifera leaf, mulberry fruit, or mulberry leaf. Moreover, serum high-density lipoprotein was significantly elevated in the rats on a high-fat diet receiving M. oleifera leaf or mulberry fruit. The antiobesity and antihyperlipidemic effects of M. oleifera leaf, mulberry fruit, and mulberry leaf provide support for the use of Moringa and mulberry as potential functional food ingredients for the prevention of obesity and hyperlipidemia.
{"title":"Polyphenols from Moringa Oleifera and Mulberry Exhibit Anti-Obesity and Anti-Hyperlipidemic Effects in Rats Fed a High-Fat Diet","authors":"R. Alqurashi, Mariam Alholaibi","doi":"10.37290/ctnr2641-452x.21:102-108","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:102-108","url":null,"abstract":"This study investigated the effects of Moringa oleifera leaf, mulberry fruit, and mulberry leaf on high-fat diet-induced changes in body weight and hyperlipidemia in rats. Healthy male rats were randomly divided into five equal-sized groups that included (i) basal diet or negative control group, (ii) high-fat diet group (basal diet + 20% fat), (iii) M. oleifera leaf-treated high-fat diet group (high-fat diet + 10% M. oleifera leaf), (iv) mulberry fruit-treated high-fat diet group (high-fat diet + 10% mulberry fruit), and (v) mulberry leaf-treated high-fat diet group (high-fat diet + 10% mulberry leaf). The rats were treated daily for 6 weeks, and body weight data and blood samples were collected for each group. At the end of the study period, body weight, total cholesterol, total triglyceride, low-density lipoprotein level, and glucose level were significantly reduced in the rats fed a high-fat diet supplemented with M. oleifera leaf, mulberry fruit, or mulberry leaf. Moreover, serum high-density lipoprotein was significantly elevated in the rats on a high-fat diet receiving M. oleifera leaf or mulberry fruit. The antiobesity and antihyperlipidemic effects of M. oleifera leaf, mulberry fruit, and mulberry leaf provide support for the use of Moringa and mulberry as potential functional food ingredients for the prevention of obesity and hyperlipidemia.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45026413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
While isoalantolactone is a sesquiterpene lactone known for its anti-inflammatory and antioxidant activities, its role in cardiac hypertrophy is unknown. This study aimed to explore the role and mechanism of isoalantolactone in cardiomyocyte hypertrophy using angiotensin II-treated H9C2 cells as a model. The Western blot analysis was used to evaluate changes in the cardiac hypertrophy marker proteins atrial natriuretic peptide and brain natriuretic peptide and the activity of nuclear-factor erythroid 2-related factor 2/mitogen-activated protein kinase signaling pathway. Rhodamine-phalloidin staining was used to measure the surface area of cardiomyocytes. Apoptosis and intracellular reactive oxygen species levels were measured by flow cytometry. The results showed that isoalantolactone inhibited angiotensin II-induced H9C2 cell hypertrophy and cardiomyocyte apoptosis and reduced intracellular reactive oxygen species and cytochrome C levels in a dose-dependent manner. Furthermore, isoalantolactone upregulated the nuclear factor erythroid 2-related factor 2/heme oxygenase-1 and inhibited extracellular signal-regulated kinase, c-Jun NH2-terminal kinase, and p38 (a stress-stimulated kinase) activation. Collectively, the findings suggest that isoalantolactone can enhance the antioxidant capacity of H9C2 cells, reduce apoptosis, and inhibit cardiac hypertrophy, and these protective effects may be related to the regulation of nuclear-factor erythroid 2-related factor 2 and mitogen-activated protein kinase pathway activity by isoalantolactone.
{"title":"Isoalantolactone Regulates the Activity of Mitogen-Activated Protein Kinases and Nuclear-Factor Erythroid 2-Related Factor 2 Pathways to Attenue Angiotensin II-Induced Cardiomyocyte Hypertrophy and Oxidative Stress","authors":"Ying Yang, Yuan Xie, Hui-nan Xu, Peng Xi, Fangfang Zheng","doi":"10.37290/ctnr2641-452x.21:72-79","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:72-79","url":null,"abstract":"While isoalantolactone is a sesquiterpene lactone known for its anti-inflammatory and antioxidant activities, its role in cardiac hypertrophy is unknown. This study aimed to explore the role and mechanism of isoalantolactone in cardiomyocyte hypertrophy using angiotensin II-treated H9C2 cells as a model. The Western blot analysis was used to evaluate changes in the cardiac hypertrophy marker proteins atrial natriuretic peptide and brain natriuretic peptide and the activity of nuclear-factor erythroid 2-related factor 2/mitogen-activated protein kinase signaling pathway. Rhodamine-phalloidin staining was used to measure the surface area of cardiomyocytes. Apoptosis and intracellular reactive oxygen species levels were measured by flow cytometry. The results showed that isoalantolactone inhibited angiotensin II-induced H9C2 cell hypertrophy and cardiomyocyte apoptosis and reduced intracellular reactive oxygen species and cytochrome C levels in a dose-dependent manner. Furthermore, isoalantolactone upregulated the nuclear factor erythroid 2-related factor 2/heme oxygenase-1 and inhibited extracellular signal-regulated kinase, c-Jun NH2-terminal kinase, and p38 (a stress-stimulated kinase) activation. Collectively, the findings suggest that isoalantolactone can enhance the antioxidant capacity of H9C2 cells, reduce apoptosis, and inhibit cardiac hypertrophy, and these protective effects may be related to the regulation of nuclear-factor erythroid 2-related factor 2 and mitogen-activated protein kinase pathway activity by isoalantolactone.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42505676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-06DOI: 10.37290/ctnr2641-452x.21:60-65
Hyung-Jin Lim, Yeong‐Seon Won, S. Bak, Soyoung Lee, Bomi Ryu, Seon-Heui Cha, Taeoh Kwon, K. Shim, Jisu Jeon, Hyuck Se Kwon, S. Lee, Seung-Jae Lee, M. Rho
We have isolated 15 bacterial strains from Aloe vera gel. Among them, we selected the Lactococcus lactis subsp. hordniae strain UAB10 based on its inhibition of IL-6-induced STAT3 activation and its ability to adhere to intestinal epithelial cells. UAB10 showed strong inhibition of interleukin-6-induced signal transducer and activator of transcription 3 activation. The UAB10 isolate showed superior adhesion to Caco-2 cells compared with KTCT 3768, a reference L. lactis subsp. hordniae strain. The UAB10 isolate displayed no hemolytic activity and more potent anti-Shigella flexneri activity than KTCT 3768. Furthermore, UAB10 was able to survive better than KTCT 3768 in oxgall-containing De Man, Rogosa, and Sharpe agar media and low-pH De Man, Rogosa, and Sharpe agar media. Finally, UAB10 did not show any resistance against the eight tested antibiotics, including ampicillin, vancomycin, and gentamicin. These results highlight the potential for the use of UAB10 as a commercial probiotic.
{"title":"In Vitro Probiotic Characteristics of the Lactococcus Lactis Subsp. Hordniae Strain UAB10 Isolated from Aloe Vera","authors":"Hyung-Jin Lim, Yeong‐Seon Won, S. Bak, Soyoung Lee, Bomi Ryu, Seon-Heui Cha, Taeoh Kwon, K. Shim, Jisu Jeon, Hyuck Se Kwon, S. Lee, Seung-Jae Lee, M. Rho","doi":"10.37290/ctnr2641-452x.21:60-65","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:60-65","url":null,"abstract":"We have isolated 15 bacterial strains from Aloe vera gel. Among them, we selected the Lactococcus lactis subsp. hordniae strain UAB10 based on its inhibition of IL-6-induced STAT3 activation and its ability to adhere to intestinal epithelial cells. UAB10 showed strong inhibition of interleukin-6-induced signal transducer and activator of transcription 3 activation. The UAB10 isolate showed superior adhesion to Caco-2 cells compared with KTCT 3768, a reference L. lactis subsp. hordniae strain. The UAB10 isolate displayed no hemolytic activity and more potent anti-Shigella flexneri activity than KTCT 3768. Furthermore, UAB10 was able to survive better than KTCT 3768 in oxgall-containing De Man, Rogosa, and Sharpe agar media and low-pH De Man, Rogosa, and Sharpe agar media. Finally, UAB10 did not show any resistance against the eight tested antibiotics, including ampicillin, vancomycin, and gentamicin. These results highlight the potential for the use of UAB10 as a commercial probiotic.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45198752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-06DOI: 10.37290/ctnr2641-452x.21:66-71
Zhong-Xi Lu, Mengqin Zhang, Xiangtao Chen, Junjie Lei
We have examined the ultrasonic ethanol extraction of Ilex latifolia Thunb flavonoids and the inhibitory effect of these flavo¬noids on xanthine oxidase. Four single-factor extraction conditions were examined: ethanol concentration (%), solid–liquid ratio (g/mL), ultrasonic extraction temperature (°C), and ultrasonic extraction time (min). With these data, the response surface methodology was adopted to optimize the ultrasonic extraction parameters. The optimal ultrasonic extraction parameters were determined to be the ethanol concentration of 49%, the solid-to-liquid ratio of 1:27, the ultrasonic extraction temperature of 74°C, and the ultrasonic extraction time of 97 min. Under the optimal conditions described ear¬lier, the experimental yield of flavonoids was 6.367% with a relative standard deviation of 1.9%, which was comparable to the theoretical extraction rate. The half maximal inhibitory concentration of flavonoids for xanthine oxidase is 5.50 mg/mL. The inhibitory effect was competitive and reversible, with an inhibitory kinetic constant Ki of 2.35 mg/mL.
{"title":"Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Ilex Latifolia Thunb by Response Surface Methodology and their Influence over the Activity of Xanthine Oxidase","authors":"Zhong-Xi Lu, Mengqin Zhang, Xiangtao Chen, Junjie Lei","doi":"10.37290/ctnr2641-452x.21:66-71","DOIUrl":"https://doi.org/10.37290/ctnr2641-452x.21:66-71","url":null,"abstract":"We have examined the ultrasonic ethanol extraction of Ilex latifolia Thunb flavonoids and the inhibitory effect of these flavo¬noids on xanthine oxidase. Four single-factor extraction conditions were examined: ethanol concentration (%), solid–liquid ratio (g/mL), ultrasonic extraction temperature (°C), and ultrasonic extraction time (min). With these data, the response surface methodology was adopted to optimize the ultrasonic extraction parameters. The optimal ultrasonic extraction parameters were determined to be the ethanol concentration of 49%, the solid-to-liquid ratio of 1:27, the ultrasonic extraction temperature of 74°C, and the ultrasonic extraction time of 97 min. Under the optimal conditions described ear¬lier, the experimental yield of flavonoids was 6.367% with a relative standard deviation of 1.9%, which was comparable to the theoretical extraction rate. The half maximal inhibitory concentration of flavonoids for xanthine oxidase is 5.50 mg/mL. The inhibitory effect was competitive and reversible, with an inhibitory kinetic constant Ki of 2.35 mg/mL.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45386819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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