Pub Date : 1900-01-01DOI: 10.1364/ntm.2023.ntu3c.7
Guangwei Hu
Differential interference contrast (DIC) microscopy is an important technique to imaging the transparent biosamples. Traditional system is bulky in 4f imaging mode, and can only perform edge detection in one dimensional, leading to anisotropic imaging. Here, I will introduce our recently developed single-shot, isotropic DIC microscopy in 2f system. This is realized by a polarization-multiplexed metalens, that can perform the computations of optical difference for edge detections in all directions. Our work promises the important compact and miniaturized microscopy for miniaturized and even portable imaging system for healthcare, environmental monitoring, and other applications.
{"title":"Single-Shot, Isotropic and Miniaturized Differential Interference Contrast (SIM-DIC) Microscopy Based on Computational Flat-Optics","authors":"Guangwei Hu","doi":"10.1364/ntm.2023.ntu3c.7","DOIUrl":"https://doi.org/10.1364/ntm.2023.ntu3c.7","url":null,"abstract":"Differential interference contrast (DIC) microscopy is an important technique to imaging the transparent biosamples. Traditional system is bulky in 4f imaging mode, and can only perform edge detection in one dimensional, leading to anisotropic imaging. Here, I will introduce our recently developed single-shot, isotropic DIC microscopy in 2f system. This is realized by a polarization-multiplexed metalens, that can perform the computations of optical difference for edge detections in all directions. Our work promises the important compact and miniaturized microscopy for miniaturized and even portable imaging system for healthcare, environmental monitoring, and other applications.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125429019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dth2a.4
Maria-Pilar Urizar, Álvaro de la Peña, E. Gambra, A. de Castro, S. Marcos, A. Curatolo
We experimentally verify the design of a low-cost scanner that performs dynamic whole-eye scans without requiring any moving component. We present beam profiles and OCT images in an ex vivo rabbit eye.
{"title":"A low-cost, non-mechanical optical beam scanner: experimental proof-of-concept for whole-eye OCT imaging","authors":"Maria-Pilar Urizar, Álvaro de la Peña, E. Gambra, A. de Castro, S. Marcos, A. Curatolo","doi":"10.1364/boda.2023.dth2a.4","DOIUrl":"https://doi.org/10.1364/boda.2023.dth2a.4","url":null,"abstract":"We experimentally verify the design of a low-cost scanner that performs dynamic whole-eye scans without requiring any moving component. We present beam profiles and OCT images in an ex vivo rabbit eye.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126453495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Romero, Jingwei Zhao, Delaney B Stratton, C. Curiel, Dongkyun Kang
We have developed a portable cross-polarized microscope for imaging human skin in vivo at high resolution. Preliminary results showed that high-NA cross-polarized microscopy could visualize individual pigmented cells for the human skin in vivo.
{"title":"Cross-Polarized Microscopy of Pigmented Cells In Vivo","authors":"R. Romero, Jingwei Zhao, Delaney B Stratton, C. Curiel, Dongkyun Kang","doi":"10.1364/ntm.2023.nw1c.3","DOIUrl":"https://doi.org/10.1364/ntm.2023.nw1c.3","url":null,"abstract":"We have developed a portable cross-polarized microscope for imaging human skin in vivo at high resolution. Preliminary results showed that high-NA cross-polarized microscopy could visualize individual pigmented cells for the human skin in vivo.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128236855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dtu1a.2
M. S. Arefin, Alexander P. Dumont, Brandon Harrison-Smith, Chetan A. Patil
A Monte Carlo-based framework used previously to investigate racial bias in pulse oximetry evaluated the influence of calibration population demographics on transcutaneous bilirubinometer accuracy in dark-skinned groups. Results support further evaluation of calibration study design.
{"title":"Computational Simulations of Transcutaneous Bilirubinometer Accuracy in Pigmented Infants","authors":"M. S. Arefin, Alexander P. Dumont, Brandon Harrison-Smith, Chetan A. Patil","doi":"10.1364/boda.2023.dtu1a.2","DOIUrl":"https://doi.org/10.1364/boda.2023.dtu1a.2","url":null,"abstract":"A Monte Carlo-based framework used previously to investigate racial bias in pulse oximetry evaluated the influence of calibration population demographics on transcutaneous bilirubinometer accuracy in dark-skinned groups. Results support further evaluation of calibration study design.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121252107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We investigate individual collagen fibrils using polarization SHG microscopy and find that polarization SHG is sensitive to changes in fibril structure and orientation.
我们使用偏振SHG显微镜观察单个胶原原纤维，发现偏振SHG对原纤维结构和取向的变化很敏感。
{"title":"Characterizing Hydration and Buckling in Individual Collagen Fibrils with Polarization Second Harmonic Generation Microscopy","authors":"Macaulay Harvey, R. Cisek, L. Kreplak, D. Tokarz","doi":"10.1364/omp.2023.om2e.3","DOIUrl":"https://doi.org/10.1364/omp.2023.om2e.3","url":null,"abstract":"We investigate individual collagen fibrils using polarization SHG microscopy and find that polarization SHG is sensitive to changes in fibril structure and orientation.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126765044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/ntm.2023.ntu3c.2
Shuhao Shen, E. Du, Kai Long, Nanguang Chena
We have implemented structured illumination in a fast-scanning confocal microscope. Significant reduction in background and noise has been accomplished. It is further augmented by image resan to enable real-time acquisition of super-resolution images.
{"title":"Fast structured illumination microscopy for deep tissue super-resolution imaging","authors":"Shuhao Shen, E. Du, Kai Long, Nanguang Chena","doi":"10.1364/ntm.2023.ntu3c.2","DOIUrl":"https://doi.org/10.1364/ntm.2023.ntu3c.2","url":null,"abstract":"We have implemented structured illumination in a fast-scanning confocal microscope. Significant reduction in background and noise has been accomplished. It is further augmented by image resan to enable real-time acquisition of super-resolution images.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126351656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/brain.2023.btu1b.6
K. Takasaki, Gaku Hatanaka, Soumya Chatterjee, P. Balaram, Taekjun Kim, Celeste Dylla, Farzaneh Abolmaali, R. Reid, Anitha Pasupathy, W. Bair, Jack Waters
3-photon excitation laser-scanning microscopy enables fluorescence imaging deep in tissue beyond the range of 2-photon microscopy. Here, we report results of functional calcium imaging of neurons with 3-photon microscopy in non-human primates. We compare 2-photon and 3-photon excitation and characterize the performance of 3-photon microscopy at depths beyond the 2-photon limit.
{"title":"Development of 3-Photon Functional Imaging in Deep Layers of Macaque Cortex","authors":"K. Takasaki, Gaku Hatanaka, Soumya Chatterjee, P. Balaram, Taekjun Kim, Celeste Dylla, Farzaneh Abolmaali, R. Reid, Anitha Pasupathy, W. Bair, Jack Waters","doi":"10.1364/brain.2023.btu1b.6","DOIUrl":"https://doi.org/10.1364/brain.2023.btu1b.6","url":null,"abstract":"3-photon excitation laser-scanning microscopy enables fluorescence imaging deep in tissue beyond the range of 2-photon microscopy. Here, we report results of functional calcium imaging of neurons with 3-photon microscopy in non-human primates. We compare 2-photon and 3-photon excitation and characterize the performance of 3-photon microscopy at depths beyond the 2-photon limit.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128746864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/omp.2023.otu3e.3
J. Torra, C. Flors
We explore the use of fluorogenic amyloid probes in two super-resolution methods. A spatial resolution of 45–70 nm, low image background and long term imaging can be achieved, expanding the toolbox of super-resolution labels.
{"title":"Unconventional fluorophores for super-resolution microscopy","authors":"J. Torra, C. Flors","doi":"10.1364/omp.2023.otu3e.3","DOIUrl":"https://doi.org/10.1364/omp.2023.otu3e.3","url":null,"abstract":"We explore the use of fluorogenic amyloid probes in two super-resolution methods. A spatial resolution of 45–70 nm, low image background and long term imaging can be achieved, expanding the toolbox of super-resolution labels.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132657072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dtu2a.1
A. Dubois
LC-OCT is an optical technique that provides three-dimensional images of the skin, in vivo, with cellular resolution (1 μm). LC-OCT can be used in dermatology for non-invasive diagnosis and study of various lesions including cancers.
{"title":"Line-field confocal optical coherence tomography (LC-OCT) for dermatology","authors":"A. Dubois","doi":"10.1364/boda.2023.dtu2a.1","DOIUrl":"https://doi.org/10.1364/boda.2023.dtu2a.1","url":null,"abstract":"LC-OCT is an optical technique that provides three-dimensional images of the skin, in vivo, with cellular resolution (1 μm). LC-OCT can be used in dermatology for non-invasive diagnosis and study of various lesions including cancers.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116782645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dth1a.6
Nisha Maheshwari, A. Marone, M. Altoe, S. H. Kim, D. Bajakian, A. Hielscher
Parameters derived from a dynamic vascular optical spectroscopy system (DVOS) characterized wound outcome in peripheral arterial disease (PAD) patients after a surgical intervention with high sensitivity and specificity by 3-4 weeks post-intervention.
{"title":"Monitoring Ulcer Healing in Patients with Peripheral Arterial Disease After Surgical Intervention Using Dynamic Vascular Optical Spectroscopy in a Preliminary Study","authors":"Nisha Maheshwari, A. Marone, M. Altoe, S. H. Kim, D. Bajakian, A. Hielscher","doi":"10.1364/boda.2023.dth1a.6","DOIUrl":"https://doi.org/10.1364/boda.2023.dth1a.6","url":null,"abstract":"Parameters derived from a dynamic vascular optical spectroscopy system (DVOS) characterized wound outcome in peripheral arterial disease (PAD) patients after a surgical intervention with high sensitivity and specificity by 3-4 weeks post-intervention.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116886922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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