Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dw1a.5
V. Perekatova, A. Kostyuk, M. Kirillin, E. Sergeeva, D. Kurakina, Olga Shemagina, A. Orlova, A. Khilov, I. Turchin
A VIS-NIR experimental setup for diffuse reflectance spectroscopy with a fiber-optic probe employing a self-calibrating approach has been created and demonstrated high robustness to various instrumental perturbations in source and detector channels.
{"title":"Broadband diffuse reflectance spectroscopy system with a self-calibrating fiber-optic probe","authors":"V. Perekatova, A. Kostyuk, M. Kirillin, E. Sergeeva, D. Kurakina, Olga Shemagina, A. Orlova, A. Khilov, I. Turchin","doi":"10.1364/boda.2023.dw1a.5","DOIUrl":"https://doi.org/10.1364/boda.2023.dw1a.5","url":null,"abstract":"A VIS-NIR experimental setup for diffuse reflectance spectroscopy with a fiber-optic probe employing a self-calibrating approach has been created and demonstrated high robustness to various instrumental perturbations in source and detector channels.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113993407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dm2a.5
Z. Guang, Y. Reddy, S. Balakirsky, Konrad Ahlin, Francisco E. Robles
We develop an epi-mode quantitative phase imaging device to characterize cell structure and dynamic activity. It is composed of an optical imager using quantitative oblique back-illumination microscopy and a hyper redundant, high degree-of-freedom snake robot.
{"title":"Epi-Mode Quantitative Phase Imaging to Characterize Cell Structure and Dynamics with a Flexible Snake Robot","authors":"Z. Guang, Y. Reddy, S. Balakirsky, Konrad Ahlin, Francisco E. Robles","doi":"10.1364/boda.2023.dm2a.5","DOIUrl":"https://doi.org/10.1364/boda.2023.dm2a.5","url":null,"abstract":"We develop an epi-mode quantitative phase imaging device to characterize cell structure and dynamic activity. It is composed of an optical imager using quantitative oblique back-illumination microscopy and a hyper redundant, high degree-of-freedom snake robot.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121806214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dtu2a.1
A. Dubois
LC-OCT is an optical technique that provides three-dimensional images of the skin, in vivo, with cellular resolution (1 μm). LC-OCT can be used in dermatology for non-invasive diagnosis and study of various lesions including cancers.
{"title":"Line-field confocal optical coherence tomography (LC-OCT) for dermatology","authors":"A. Dubois","doi":"10.1364/boda.2023.dtu2a.1","DOIUrl":"https://doi.org/10.1364/boda.2023.dtu2a.1","url":null,"abstract":"LC-OCT is an optical technique that provides three-dimensional images of the skin, in vivo, with cellular resolution (1 μm). LC-OCT can be used in dermatology for non-invasive diagnosis and study of various lesions including cancers.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116782645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dth1a.6
Nisha Maheshwari, A. Marone, M. Altoe, S. H. Kim, D. Bajakian, A. Hielscher
Parameters derived from a dynamic vascular optical spectroscopy system (DVOS) characterized wound outcome in peripheral arterial disease (PAD) patients after a surgical intervention with high sensitivity and specificity by 3-4 weeks post-intervention.
{"title":"Monitoring Ulcer Healing in Patients with Peripheral Arterial Disease After Surgical Intervention Using Dynamic Vascular Optical Spectroscopy in a Preliminary Study","authors":"Nisha Maheshwari, A. Marone, M. Altoe, S. H. Kim, D. Bajakian, A. Hielscher","doi":"10.1364/boda.2023.dth1a.6","DOIUrl":"https://doi.org/10.1364/boda.2023.dth1a.6","url":null,"abstract":"Parameters derived from a dynamic vascular optical spectroscopy system (DVOS) characterized wound outcome in peripheral arterial disease (PAD) patients after a surgical intervention with high sensitivity and specificity by 3-4 weeks post-intervention.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116886922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dw4a.4
Elizabeth Aguilar, E. Guevara, Guadalupe Donjuán-Loredo, R. Espinosa‐Tanguma, Roberto Salgado Delgado, M. Ramírez-Elías
Steatosis is when the liver accumulates more than 5% of its total weight in fat. Raman imaging was used to evaluate the molecular content of ex vivo rat liver tissue samples, where proteins and fatty acids were found in abundance.
{"title":"Analysis of Non-Alcoholic Fatty Liver Through Intensity Maps Applying Raman Spectroscopy","authors":"Elizabeth Aguilar, E. Guevara, Guadalupe Donjuán-Loredo, R. Espinosa‐Tanguma, Roberto Salgado Delgado, M. Ramírez-Elías","doi":"10.1364/boda.2023.dw4a.4","DOIUrl":"https://doi.org/10.1364/boda.2023.dw4a.4","url":null,"abstract":"Steatosis is when the liver accumulates more than 5% of its total weight in fat. Raman imaging was used to evaluate the molecular content of ex vivo rat liver tissue samples, where proteins and fatty acids were found in abundance.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"150 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123229051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/ntm.2023.ntu3c.7
Guangwei Hu
Differential interference contrast (DIC) microscopy is an important technique to imaging the transparent biosamples. Traditional system is bulky in 4f imaging mode, and can only perform edge detection in one dimensional, leading to anisotropic imaging. Here, I will introduce our recently developed single-shot, isotropic DIC microscopy in 2f system. This is realized by a polarization-multiplexed metalens, that can perform the computations of optical difference for edge detections in all directions. Our work promises the important compact and miniaturized microscopy for miniaturized and even portable imaging system for healthcare, environmental monitoring, and other applications.
{"title":"Single-Shot, Isotropic and Miniaturized Differential Interference Contrast (SIM-DIC) Microscopy Based on Computational Flat-Optics","authors":"Guangwei Hu","doi":"10.1364/ntm.2023.ntu3c.7","DOIUrl":"https://doi.org/10.1364/ntm.2023.ntu3c.7","url":null,"abstract":"Differential interference contrast (DIC) microscopy is an important technique to imaging the transparent biosamples. Traditional system is bulky in 4f imaging mode, and can only perform edge detection in one dimensional, leading to anisotropic imaging. Here, I will introduce our recently developed single-shot, isotropic DIC microscopy in 2f system. This is realized by a polarization-multiplexed metalens, that can perform the computations of optical difference for edge detections in all directions. Our work promises the important compact and miniaturized microscopy for miniaturized and even portable imaging system for healthcare, environmental monitoring, and other applications.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125429019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dtu1a.2
M. S. Arefin, Alexander P. Dumont, Brandon Harrison-Smith, Chetan A. Patil
A Monte Carlo-based framework used previously to investigate racial bias in pulse oximetry evaluated the influence of calibration population demographics on transcutaneous bilirubinometer accuracy in dark-skinned groups. Results support further evaluation of calibration study design.
{"title":"Computational Simulations of Transcutaneous Bilirubinometer Accuracy in Pigmented Infants","authors":"M. S. Arefin, Alexander P. Dumont, Brandon Harrison-Smith, Chetan A. Patil","doi":"10.1364/boda.2023.dtu1a.2","DOIUrl":"https://doi.org/10.1364/boda.2023.dtu1a.2","url":null,"abstract":"A Monte Carlo-based framework used previously to investigate racial bias in pulse oximetry evaluated the influence of calibration population demographics on transcutaneous bilirubinometer accuracy in dark-skinned groups. Results support further evaluation of calibration study design.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121252107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Romero, Jingwei Zhao, Delaney B Stratton, C. Curiel, Dongkyun Kang
We have developed a portable cross-polarized microscope for imaging human skin in vivo at high resolution. Preliminary results showed that high-NA cross-polarized microscopy could visualize individual pigmented cells for the human skin in vivo.
{"title":"Cross-Polarized Microscopy of Pigmented Cells In Vivo","authors":"R. Romero, Jingwei Zhao, Delaney B Stratton, C. Curiel, Dongkyun Kang","doi":"10.1364/ntm.2023.nw1c.3","DOIUrl":"https://doi.org/10.1364/ntm.2023.nw1c.3","url":null,"abstract":"We have developed a portable cross-polarized microscope for imaging human skin in vivo at high resolution. Preliminary results showed that high-NA cross-polarized microscopy could visualize individual pigmented cells for the human skin in vivo.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128236855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.jw2a.2
Bingxue Liu, D. Boas, Xiaojun Cheng
We present a short-separation speckle contrast optical spectroscopy (ss-SCOS) system to enhance the static and slow tissue components with the multi-exposure time-based speckle contrast model to extract both the slow tissue and fast blood flow dynamics.
{"title":"Measurements of slow tissue dynamics with short-separation speckle contrast optical spectroscopy","authors":"Bingxue Liu, D. Boas, Xiaojun Cheng","doi":"10.1364/boda.2023.jw2a.2","DOIUrl":"https://doi.org/10.1364/boda.2023.jw2a.2","url":null,"abstract":"We present a short-separation speckle contrast optical spectroscopy (ss-SCOS) system to enhance the static and slow tissue components with the multi-exposure time-based speckle contrast model to extract both the slow tissue and fast blood flow dynamics.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128199889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/brain.2023.btu1b.6
K. Takasaki, Gaku Hatanaka, Soumya Chatterjee, P. Balaram, Taekjun Kim, Celeste Dylla, Farzaneh Abolmaali, R. Reid, Anitha Pasupathy, W. Bair, Jack Waters
3-photon excitation laser-scanning microscopy enables fluorescence imaging deep in tissue beyond the range of 2-photon microscopy. Here, we report results of functional calcium imaging of neurons with 3-photon microscopy in non-human primates. We compare 2-photon and 3-photon excitation and characterize the performance of 3-photon microscopy at depths beyond the 2-photon limit.
{"title":"Development of 3-Photon Functional Imaging in Deep Layers of Macaque Cortex","authors":"K. Takasaki, Gaku Hatanaka, Soumya Chatterjee, P. Balaram, Taekjun Kim, Celeste Dylla, Farzaneh Abolmaali, R. Reid, Anitha Pasupathy, W. Bair, Jack Waters","doi":"10.1364/brain.2023.btu1b.6","DOIUrl":"https://doi.org/10.1364/brain.2023.btu1b.6","url":null,"abstract":"3-photon excitation laser-scanning microscopy enables fluorescence imaging deep in tissue beyond the range of 2-photon microscopy. Here, we report results of functional calcium imaging of neurons with 3-photon microscopy in non-human primates. We compare 2-photon and 3-photon excitation and characterize the performance of 3-photon microscopy at depths beyond the 2-photon limit.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128746864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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