Pub Date : 1900-01-01DOI: 10.1364/oma.2023.ath1d.2
S. N. Chormaic, G. Tkachenko, Cindy Esporlas, I. Sanskriti, V. G. Truong
We discuss the manipulation of gold-silica Janus particles in the evanescent field of optical nanofibers. Comparison between the trapping efficiency of silica particles is included. In particular, we pay attention to thermal effects due to the gold coating and note that the gold acts as an optical sail for particle translation.
{"title":"Manipulation of Janus Particles using Optical Nanofibers","authors":"S. N. Chormaic, G. Tkachenko, Cindy Esporlas, I. Sanskriti, V. G. Truong","doi":"10.1364/oma.2023.ath1d.2","DOIUrl":"https://doi.org/10.1364/oma.2023.ath1d.2","url":null,"abstract":"We discuss the manipulation of gold-silica Janus particles in the evanescent field of optical nanofibers. Comparison between the trapping efficiency of silica particles is included. In particular, we pay attention to thermal effects due to the gold coating and note that the gold acts as an optical sail for particle translation.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122764959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/omp.2023.oth1e.2
Hengze You, C. L. Anyi, Liaoliao Wei, Tong Ling
We applied iSCAT microscopy to image the Brownian movement of nanoparticles in live cell culture. Utilizing autocorrelation analysis, we demonstrated mapping of nanoparticle binding interactions with the plasma membrane in a wide field.
{"title":"Imaging Nanoparticle Binding to Cell Membrane through Wide-field Interferometric Scattering Microscopy","authors":"Hengze You, C. L. Anyi, Liaoliao Wei, Tong Ling","doi":"10.1364/omp.2023.oth1e.2","DOIUrl":"https://doi.org/10.1364/omp.2023.oth1e.2","url":null,"abstract":"We applied iSCAT microscopy to image the Brownian movement of nanoparticles in live cell culture. Utilizing autocorrelation analysis, we demonstrated mapping of nanoparticle binding interactions with the plasma membrane in a wide field.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122475741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/ntm.2023.nth1c.4
George T. Funkenbusch, Cindy X. Chen, A. Wax
Two-wavelength phase microscopy enables quantification of hemoglobin concentration and height of fast-flowing cells in a flow cytometer. This approach shows great potential for point-of-care diagnosis of blood disorders.
{"title":"Dual-Wavelength Holographic Cytometry for Red Blood Cell Disease Diagnosis","authors":"George T. Funkenbusch, Cindy X. Chen, A. Wax","doi":"10.1364/ntm.2023.nth1c.4","DOIUrl":"https://doi.org/10.1364/ntm.2023.nth1c.4","url":null,"abstract":"Two-wavelength phase microscopy enables quantification of hemoglobin concentration and height of fast-flowing cells in a flow cytometer. This approach shows great potential for point-of-care diagnosis of blood disorders.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123058721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dtu2a.5
Clara Zaccaria, Asiye Malkoç, Beatrice Vignoli, M. Canossa, L. Pavesi
Memory relies on the firing of simultaneously activated neurons (engram), whose synapses are strengthened by long-term potentiation mechanisms. Optogenetic tools and a fluorescence probe to map synaptic engrams, were combined with a digital light processor device (DLP), to create in-vitro engrams and study populations of potentiated spines.
{"title":"A Platform for Single Cell Optogenetics to Study Synaptic Engrams in Vitro","authors":"Clara Zaccaria, Asiye Malkoç, Beatrice Vignoli, M. Canossa, L. Pavesi","doi":"10.1364/boda.2023.dtu2a.5","DOIUrl":"https://doi.org/10.1364/boda.2023.dtu2a.5","url":null,"abstract":"Memory relies on the firing of simultaneously activated neurons (engram), whose synapses are strengthened by long-term potentiation mechanisms. Optogenetic tools and a fluorescence probe to map synaptic engrams, were combined with a digital light processor device (DLP), to create in-vitro engrams and study populations of potentiated spines.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"356 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123109366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dw1a.6
A. Formozov, Joseph Lamarre, A. Dieter, Lucas Majeau, J. Simon Wiegert, Kathy Beaudette
Here, we present a wideband multimode circulator optimized for high transfer and transmittance of light, and low-autofluorescence. It is suitable for fiber photometry and optogenetics, allowing the inference of brain activity in awake mouse.
{"title":"All-fiber photometry based on a novel, low-autofluorescence wideband multimode circulator","authors":"A. Formozov, Joseph Lamarre, A. Dieter, Lucas Majeau, J. Simon Wiegert, Kathy Beaudette","doi":"10.1364/boda.2023.dw1a.6","DOIUrl":"https://doi.org/10.1364/boda.2023.dw1a.6","url":null,"abstract":"Here, we present a wideband multimode circulator optimized for high transfer and transmittance of light, and low-autofluorescence. It is suitable for fiber photometry and optogenetics, allowing the inference of brain activity in awake mouse.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117164242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/omp.2023.otu3e.4
Ruqiang Liang, Junqing Sun, Ryan G. Natan, Boyu Lyu, Guoqiang Yu, Yihan Jin, Hwai-Jong Cheng, K. Zito, Na Ji, Lin Tian
A splittable genetically encoded intensimetric fluorescent sensor for glutamate is engineered. It can reconstitute across synaptic clefts and report glutamate transients when the nonfluorescent halves are targeted to pre- and post-synaptic neurons in a circuit.
{"title":"Fluorescent imaging of synaptic glutamate transients in defined neuronal circuits","authors":"Ruqiang Liang, Junqing Sun, Ryan G. Natan, Boyu Lyu, Guoqiang Yu, Yihan Jin, Hwai-Jong Cheng, K. Zito, Na Ji, Lin Tian","doi":"10.1364/omp.2023.otu3e.4","DOIUrl":"https://doi.org/10.1364/omp.2023.otu3e.4","url":null,"abstract":"A splittable genetically encoded intensimetric fluorescent sensor for glutamate is engineered. It can reconstitute across synaptic clefts and report glutamate transients when the nonfluorescent halves are targeted to pre- and post-synaptic neurons in a circuit.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"240 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115504240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/brain.2023.bw3b.5
Ivan Vishniakou, Johannes D. Seelig
We develop a differentiable optics approach for modeling light propagation through a high N.A. microscope objective based on efficient computational optimization of the Debye-Wolf diffraction integral. We apply the technique for wavefront sensing for adaptive optics without guide stars and light shaping in a two-photon microscope.
{"title":"Differentiable Adaptive Optics and Light Shaping in Two-Photon Microscopy","authors":"Ivan Vishniakou, Johannes D. Seelig","doi":"10.1364/brain.2023.bw3b.5","DOIUrl":"https://doi.org/10.1364/brain.2023.bw3b.5","url":null,"abstract":"We develop a differentiable optics approach for modeling light propagation through a high N.A. microscope objective based on efficient computational optimization of the Debye-Wolf diffraction integral. We apply the technique for wavefront sensing for adaptive optics without guide stars and light shaping in a two-photon microscope.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"151 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115575453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.jtu4b.26
Hao Guo, A. Stueck, A. B. Tikhomirov, H. Zeng, I. Alwayn, B. Gala-Lopez, A. Mahadevan-Jansen, A. Locke, K. Hewitt
We analyzed 70 human liver tissue samples using a filter-based 1064-nm Raman system and Oil Red O staining. The system effectively differentiated low/high-risk liver macrosteatosis, with an area under the operating characteristic curve of 0.92.
{"title":"Evaluation of Steatosis in Human Liver Specimens Using an Ambient Light-compatible Raman Spectroscopy Approach","authors":"Hao Guo, A. Stueck, A. B. Tikhomirov, H. Zeng, I. Alwayn, B. Gala-Lopez, A. Mahadevan-Jansen, A. Locke, K. Hewitt","doi":"10.1364/boda.2023.jtu4b.26","DOIUrl":"https://doi.org/10.1364/boda.2023.jtu4b.26","url":null,"abstract":"We analyzed 70 human liver tissue samples using a filter-based 1064-nm Raman system and Oil Red O staining. The system effectively differentiated low/high-risk liver macrosteatosis, with an area under the operating characteristic curve of 0.92.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115282271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Young and old murine cortical tissue in fresh, snap frozen, and DMSO-preserved form were investigated with two-photon microscopy. Shorter mean NADH and FAD lifetimes were found in old cortical tissue compared to young.
{"title":"Fluorescence Lifetime Imaging of NADH and FAD in Ex Vivo Young and Old Mouse Cortical Tissue","authors":"P. M. Díaz, Narasimhan Rajaram","doi":"10.1364/omp.2023.om2e.2","DOIUrl":"https://doi.org/10.1364/omp.2023.om2e.2","url":null,"abstract":"Young and old murine cortical tissue in fresh, snap frozen, and\u0000 DMSO-preserved form were investigated with two-photon microscopy.\u0000 Shorter mean NADH and FAD lifetimes were found in old cortical tissue\u0000 compared to young.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125418822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this computational study active dumbbell-shaped particles are held in optical traps, and their arrangements and dynamics studied. The richness of resultant physical behaviour is explored for different refractive index particles.
{"title":"Dynamics of Active Particles Confined in Optical Traps","authors":"Michael J. O'Donnell, Thomas S. Morling, S. Hanna","doi":"10.1364/oma.2023.am4d.3","DOIUrl":"https://doi.org/10.1364/oma.2023.am4d.3","url":null,"abstract":"In this computational study active dumbbell-shaped particles are held in optical traps, and their arrangements and dynamics studied. The richness of resultant physical behaviour is explored for different refractive index particles.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"195 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128198347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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