Environmental Technology & Innovation最新文献_第8页

Effect of freeze-thaw cycle on carbon fixation by soil microbes 冻融循环对土壤微生物固碳作用的影响

IF 7.1 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-12-19 DOI: 10.1016/j.eti.2025.104713

Lian He , Yujuan Wen , Zhipeng Zhang , Xiaoming Song

The freeze-thaw cycle (FTC) is a common periodic process of soil change in middle and high latitudes and high altitudes. FTC can affect microbial functional genes, plant carbon input patterns and physicochemical protection mechanisms of soil organic carbon. FTC significantly affected soil carbon cycle and microbial carbon sequestration by changing soil porosity, temperature, water content and aggregate structure. However, current research lacks sufficient discussion on the long-term effects of FTC the functional mechanisms of microbial communities and soil carbon components, and the coupled effects of multiple environmental factors. This paper introduces the efficiency of the microbial carbon pump (MCP) and its related microbial indicators as key parameters into the analytical framework of the carbon cycle model. The review encompasses the following aspects: (1) the mechanisms of carbon fixation by soil microorganism; (2) response and changes in the composition and activity of soil microbial communities under freeze-thaw cyclic stress; (3) the influence of FTC on the carbon cycle under different soil depth conditions; (4) how FTC jointly affect soil carbon dynamics by regulating microbial carbon sequestration-related functional genes (involved in substrate utilization preference and carbon use efficiency) and their interactions with plant organic carbon input patterns. This review systematically integrates research findings on the effects of FTC on soil carbon pools and microbial carbon sequestration, clarifies core mechanisms and research gaps, and provides key support for theoretical refinement, ecological conservation, and carbon sink management in related fields, promoting interdisciplinary research integration.

冻融循环是中高纬度和高海拔地区土壤变化的一种常见的周期性过程。FTC可以影响微生物功能基因、植物碳输入模式和土壤有机碳的理化保护机制。FTC通过改变土壤孔隙度、温度、含水量和团聚体结构显著影响土壤碳循环和微生物固碳。然而，目前的研究缺乏对FTC的长期影响、微生物群落与土壤碳组分的作用机制以及多种环境因素的耦合效应的充分讨论。本文将微生物碳泵（MCP）效率及其相关微生物指标作为关键参数引入碳循环模型的分析框架。本文主要从以下几个方面进行了综述：(1)土壤微生物的固碳机制；(2)冻融循环胁迫下土壤微生物群落组成与活性的响应与变化；(3)不同土壤深度条件下FTC对碳循环的影响；(4) FTC如何通过调节微生物固碳相关功能基因（涉及底物利用偏好和碳利用效率）及其与植物有机碳输入模式的相互作用，共同影响土壤碳动态。本文系统整合了FTC对土壤碳库和微生物固碳影响的研究成果，明确了核心机制和研究空白，为相关领域的理论完善、生态保护和碳汇管理提供了关键支撑，促进了跨学科研究的整合。

{"title":"Effect of freeze-thaw cycle on carbon fixation by soil microbes","authors":"Lian He , Yujuan Wen , Zhipeng Zhang , Xiaoming Song","doi":"10.1016/j.eti.2025.104713","DOIUrl":"10.1016/j.eti.2025.104713","url":null,"abstract":"<div><div>The freeze-thaw cycle (FTC) is a common periodic process of soil change in middle and high latitudes and high altitudes. FTC can affect microbial functional genes, plant carbon input patterns and physicochemical protection mechanisms of soil organic carbon. FTC significantly affected soil carbon cycle and microbial carbon sequestration by changing soil porosity, temperature, water content and aggregate structure. However, current research lacks sufficient discussion on the long-term effects of FTC the functional mechanisms of microbial communities and soil carbon components, and the coupled effects of multiple environmental factors. This paper introduces the efficiency of the microbial carbon pump (MCP) and its related microbial indicators as key parameters into the analytical framework of the carbon cycle model. The review encompasses the following aspects: (1) the mechanisms of carbon fixation by soil microorganism; (2) response and changes in the composition and activity of soil microbial communities under freeze-thaw cyclic stress; (3) the influence of FTC on the carbon cycle under different soil depth conditions; (4) how FTC jointly affect soil carbon dynamics by regulating microbial carbon sequestration-related functional genes (involved in substrate utilization preference and carbon use efficiency) and their interactions with plant organic carbon input patterns. This review systematically integrates research findings on the effects of FTC on soil carbon pools and microbial carbon sequestration, clarifies core mechanisms and research gaps, and provides key support for theoretical refinement, ecological conservation, and carbon sink management in related fields, promoting interdisciplinary research integration.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"41 ","pages":"Article 104713"},"PeriodicalIF":7.1,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145938950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Straw incorporation combined with Cd/As resistant microbial inoculation reduces arsenic and cadmium bioavailability and translocation in Ipomoea aquatica (water spinach) 秸秆还田配合抗Cd/As微生物接种可降低水菠菜中砷和镉的生物利用度和转运

IF 7.1 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-12-18 DOI: 10.1016/j.eti.2025.104709

Chen Huojun , Hou Yuting , Farhan Nabi , Weizhi Li , Rakhwe Kama , Huashou Li

Straw undergoes a microbial degradation process in soil, influencing heavy metal (HM) speciation through humification and mineralization. This study investigates the combined effect of straw incorporation and Cd/As-resistant microbial inocula on arsenic (As) and cadmium (Cd) co-contaminated soil remediation and their accumulation in water spinach. A pot experiment was conducted to assess changes in soil As/Cd speciation, organic carbon fractions, and water spinach uptake under straw incorporation and microbial inocula, such as the metal-tolerant strains Pseudomonas sp. C and Bacillus sp. D, to assess their impact on phytoremediation efficiency. The results showed that microbial agents C and D significantly increased soil microbial biomass carbon (MBC), while microbial agent C also enhanced soil total organic carbon (TOC) and dissolved organic carbon (DOC) content. Microbial agent D reduced the mobility of As and Cd, shifting As to crystalline iron-aluminum oxide-bound forms and Cd to iron-manganese oxide-bound and organic/sulfide-bound forms. All microbial agents promoted humic acid (HA) accumulation and influenced metal uptake patterns. Microbial agent C enhanced Cd translocation to all plant parts and increased stem As uptake while reducing leaf As content. Microbial agent D reduced As accumulation in roots and leaves but increased stem translocation. These findings suggest that combining straw with microbial inocula improves soil health, reduces HM bioavailability and mobility, offering a sustainable strategy for managing Cd/As co-contaminated agricultural soils.

秸秆在土壤中经历微生物降解过程，通过腐殖化和矿化影响重金属形态。研究了秸秆还田和抗Cd/As微生物接种对砷镉共污染土壤修复及其在菠菜中积累的影响。采用盆栽试验研究了秸秆还田和接种耐金属菌株假单胞菌C和芽孢杆菌D对土壤As/Cd形态、有机碳组分和菠菜水分吸收的影响，并探讨了它们对植物修复效率的影响。结果表明，微生物剂C和D显著提高了土壤微生物生物量碳（MBC），微生物剂C也显著提高了土壤总有机碳（TOC）和溶解有机碳（DOC）含量。微生物剂D降低了砷和镉的迁移率，将砷转变为结晶铁铝氧化物结合形式，将镉转变为铁锰氧化物结合形式和有机/硫化物结合形式。所有微生物剂均促进腐殖酸（HA）积累，并影响金属吸收模式。微生物剂C促进了镉在植株各部位的转运，增加了茎对砷的吸收，降低了叶片砷含量。微生物剂D减少了根和叶中砷的积累，但增加了茎的转运。这些结果表明，秸秆与微生物接种可改善土壤健康，降低HM的生物有效性和流动性，为治理Cd/As共污染的农业土壤提供了一种可持续的策略。

{"title":"Straw incorporation combined with Cd/As resistant microbial inoculation reduces arsenic and cadmium bioavailability and translocation in Ipomoea aquatica (water spinach)","authors":"Chen Huojun , Hou Yuting , Farhan Nabi , Weizhi Li , Rakhwe Kama , Huashou Li","doi":"10.1016/j.eti.2025.104709","DOIUrl":"10.1016/j.eti.2025.104709","url":null,"abstract":"<div><div>Straw undergoes a microbial degradation process in soil, influencing heavy metal (HM) speciation through humification and mineralization. This study investigates the combined effect of straw incorporation and Cd/As-resistant microbial inocula on arsenic (As) and cadmium (Cd) co-contaminated soil remediation and their accumulation in water spinach. A pot experiment was conducted to assess changes in soil As/Cd speciation, organic carbon fractions, and water spinach uptake under straw incorporation and microbial inocula, such as the metal-tolerant strains <em>Pseudomonas</em> sp. C and <em>Bacillus</em> sp. D, to assess their impact on phytoremediation efficiency. The results showed that microbial agents C and D significantly increased soil microbial biomass carbon (MBC), while microbial agent C also enhanced soil total organic carbon (TOC) and dissolved organic carbon (DOC) content. Microbial agent D reduced the mobility of As and Cd, shifting As to crystalline iron-aluminum oxide-bound forms and Cd to iron-manganese oxide-bound and organic/sulfide-bound forms. All microbial agents promoted humic acid (HA) accumulation and influenced metal uptake patterns. Microbial agent C enhanced Cd translocation to all plant parts and increased stem As uptake while reducing leaf As content. Microbial agent D reduced As accumulation in roots and leaves but increased stem translocation. These findings suggest that combining straw with microbial inocula improves soil health, reduces HM bioavailability and mobility, offering a sustainable strategy for managing Cd/As co-contaminated agricultural soils.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"41 ","pages":"Article 104709"},"PeriodicalIF":7.1,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing genotypic-QMRA framework for protozoan health risk assessment in urban recreational waters 建立城市游憩水域原生动物健康风险评价的基因型- qmra框架

IF 7.1 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-12-18 DOI: 10.1016/j.eti.2025.104712

Zixuan Zhang , Miaomiao Teng , Xiaoran Gong , Lihui Zhao , Yunxia Li , Hongbo Ma , Fuwei Zhang

Cryptosporidium and Giardia are globally significant protozoan pathogens, frequently linked to recreational waterborne outbreaks. This study introduces a genotypic-Quantitative Microbial Risk Assessment (QMRA) framework to evaluate the occurrence, diversity, and health risks of Cryptosporidium and Giardia in urban recreational waters. A total of 104 water samples were collected from three exposure zones over two recreational seasons in 2024 and 2025. Cryptosporidium oocysts and Giardia cysts were detected in 84.6 % and 93.3 % of samples, respectively. Genotyping identified six Cryptosporidium species including C. parvum (10.6 %) and C. andersoni (14.4 %) and three Giardia assemblages, with assemblage A (12.5 %) being dominant. Risk model showed that the annual disease burden associated with recreational exposure exceeded the World Health Organization (WHO) threshold of 1.0 × 10⁻⁶ Disability-Adjusted Life Years (DALYs) per person per year. Boating and wading activities contributed the highest risks, with estimated burdens of 1.31 and 1.25 DALYs per million persons for Cryptosporidium, and 0.71 and 0.68 DALYs pppy (per person per year) for Giardia, respectively. Sensitivity analysis identified the fraction of exposed population and exposure frequency as the most influential parameters. Human fecal contamination, indicated by a moderate correlation between HF183 markers and protozoan concentrations (r = 0.34 for Giardia), was identified as the main source of contamination. This Genotypic-QMRA approach provides a novel and scalable tool for pathogen surveillance and health risk management in urban aquatic environments.

隐孢子虫和贾第鞭毛虫是全球重要的原生动物病原体，经常与娱乐性水传播疫情有关。本研究引入基因型-定量微生物风险评估（QMRA）框架，对城市游憩水域隐孢子虫和贾第鞭毛虫的发生、多样性和健康风险进行评估。在2024年和2025年的两个休闲季节，从三个暴露区共收集了104个水样。隐孢子虫卵囊阳性率为84.6% %，贾第鞭毛虫卵囊阳性率为93.3% %。基因分型鉴定出细小隐孢子虫（10.6% %）和安德氏隐孢子虫（14.4% %）等6种隐孢子虫和贾第鞭毛虫3个组合，其中A组合占主导地位（12. %）。风险模型显示，与娱乐暴露相关的年疾病负担超过了世界卫生组织（WHO）的阈值1.0 × 10⁻26每人每年的残疾调整生命年（DALYs）。划船和涉水活动的风险最高，隐孢子虫的估计负担为每百万人1.31和1.25 DALYs，贾第鞭毛虫的估计负担为每百万人0.71和0.68 DALYs（每人每年）。敏感性分析发现，暴露人群比例和暴露频率是影响最大的参数。人类粪便污染被确定为主要污染源，HF183标记物与原生动物浓度之间存在中度相关性（贾第鞭毛虫r = 0.34）。这种基因型- qmra方法为城市水生环境中的病原体监测和健康风险管理提供了一种新颖且可扩展的工具。

{"title":"Establishing genotypic-QMRA framework for protozoan health risk assessment in urban recreational waters","authors":"Zixuan Zhang , Miaomiao Teng , Xiaoran Gong , Lihui Zhao , Yunxia Li , Hongbo Ma , Fuwei Zhang","doi":"10.1016/j.eti.2025.104712","DOIUrl":"10.1016/j.eti.2025.104712","url":null,"abstract":"<div><div><em>Cryptosporidium</em> and <em>Giardia</em> are globally significant protozoan pathogens, frequently linked to recreational waterborne outbreaks. This study introduces a genotypic-Quantitative Microbial Risk Assessment (QMRA) framework to evaluate the occurrence, diversity, and health risks of <em>Cryptosporidium</em> and <em>Giardia</em> in urban recreational waters. A total of 104 water samples were collected from three exposure zones over two recreational seasons in 2024 and 2025. <em>Cryptosporidium</em> oocysts and <em>Giardia</em> cysts were detected in 84.6 % and 93.3 % of samples, respectively. Genotyping identified six <em>Cryptosporidium</em> species including <em>C. parvum</em> (10.6 %) and <em>C. andersoni</em> (14.4 %) and three <em>Giardia</em> assemblages, with assemblage A (12.5 %) being dominant. Risk model showed that the annual disease burden associated with recreational exposure exceeded the World Health Organization (WHO) threshold of 1.0 × 10⁻⁶ Disability-Adjusted Life Years (DALYs) per person per year. Boating and wading activities contributed the highest risks, with estimated burdens of 1.31 and 1.25 DALYs per million persons for <em>Cryptosporidium</em>, and 0.71 and 0.68 DALYs pppy (per person per year) for <em>Giardia</em>, respectively. Sensitivity analysis identified the fraction of exposed population and exposure frequency as the most influential parameters. Human fecal contamination, indicated by a moderate correlation between HF183 markers and protozoan concentrations (r = 0.34 for <em>Giardia</em>), was identified as the main source of contamination. This Genotypic-QMRA approach provides a novel and scalable tool for pathogen surveillance and health risk management in urban aquatic environments.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"41 ","pages":"Article 104712"},"PeriodicalIF":7.1,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of coexisting ions on Cr(VI) interfacial structures: A charge distribution multi-site complexation (CD-MUSIC) modeling study 共存离子对Cr（VI）界面结构的影响：电荷分布多位点络合（CD-MUSIC）模型研究

IF 7.1 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-12-17 DOI: 10.1016/j.eti.2025.104710

Shenmin Zhou, Xinru Zhang, Shan Hu

Despite the fact that chromate [Cr(VI)] adsorption mechanisms have been extensively studied, there is still a lack of knowledge on the influence of typical coexisting ions on Cr(VI) surface structures. Ferrihydrite (Fh), a common naturally occurring material with enormous active sites, and titanium dioxide (TiO₂), the basis for many modified nanomaterials in wastewater treatment, were chosen as the interaction surfaces. Although TiO₂ has a higher specific surface area (196 m²·g⁻¹) than Fh (135 m²·g⁻¹), Cr(VI) adsorption on Fh significantly surpassed that of TiO₂ within pH 3.0 ∼ 8.0. The results of FTIR, XPS, and charge distribution multi-site complexation (CD-MUSIC) model testified that Cr(VI) retention on Fh occurred through monodentate and bidentate inner-sphere adsorption, whereas the inner- and outer-sphere complexes were formed on TiO₂. ClO₄^-, HCO₃^-, and SO₄^2-, which mostly undergo outer-sphere adsorption, could barely interfere with Cr(VI) adsorption on Fh, but the outer-sphere adsorption of Cr(VI) on TiO₂ was greatly inhibited. By creating a cation-bridged surface complex, Cd²⁺ significantly improved the adsorption and immobilization stability of Cr(VI), whereas Fe³⁺ competed with Cr(VI) for the surface sites. According to desorption experiments, the Cr(VI) bidentate species was less likely to redissolve than the monodentate and outer-sphere complexes. Arsenate severely inhibited Cr(VI) adsorption but increased the percentage of monodentate adsorption of Cr(VI) on Fh, which in turn improved the mobility of Cr(VI). Our findings provide molecular-level surface structures and predictive models for Cr(VI) removal in the presence of coexisting ions, which is important in the co-removal of multiple pollutants.

尽管对铬酸盐[Cr(VI)]的吸附机理进行了广泛的研究，但对典型共存离子对Cr（VI）表面结构的影响仍缺乏了解。选择天然存在的具有大量活性位点的水合铁（Fh）和废水处理中许多改性纳米材料的基础二氧化钛（TiO2）作为相互作用表面。虽然TiO2的比表面积（196 m2·g−1）高于Fh(135 m2·g−1)，但在pH 3.0 ~ 8.0范围内，Fh对Cr（VI）的吸附明显超过TiO2。FTIR、XPS和电荷分布多位点络合（CD-MUSIC）模型的结果证明，Cr（VI）在Fh上的保留是通过单齿和双齿的球内吸附实现的，而在TiO2上形成了球内和球外络合物。主要进行外球吸附的ClO4-、HCO3-和SO42-对Fh上Cr（VI）的吸附几乎没有干扰，但对TiO2上Cr（VI）的外球吸附受到了极大的抑制。通过形成阳离子桥接的表面络合物，Cd2+显著提高了Cr（VI）的吸附和固定化稳定性，而Fe3+则与Cr（VI）竞争表面位置。解吸实验表明，双齿Cr（VI）比单齿Cr（VI）和外球Cr（VI）配合物更不容易再溶解。砷酸盐严重抑制了Cr（VI）的吸附，但增加了Fh对Cr（VI）的单齿吸附率，从而提高了Cr（VI）的迁移率。我们的研究结果为共存离子存在下的Cr（VI）去除提供了分子水平的表面结构和预测模型，这在多种污染物的共去除中很重要。

{"title":"Effects of coexisting ions on Cr(VI) interfacial structures: A charge distribution multi-site complexation (CD-MUSIC) modeling study","authors":"Shenmin Zhou, Xinru Zhang, Shan Hu","doi":"10.1016/j.eti.2025.104710","DOIUrl":"10.1016/j.eti.2025.104710","url":null,"abstract":"<div><div>Despite the fact that chromate [Cr(VI)] adsorption mechanisms have been extensively studied, there is still a lack of knowledge on the influence of typical coexisting ions on Cr(VI) surface structures. Ferrihydrite (Fh), a common naturally occurring material with enormous active sites, and titanium dioxide (TiO<sub>2</sub>), the basis for many modified nanomaterials in wastewater treatment, were chosen as the interaction surfaces. Although TiO<sub>2</sub> has a higher specific surface area (196 m<sup>2</sup>·g<sup>−1</sup>) than Fh (135 m<sup>2</sup>·g<sup>−1</sup>), Cr(VI) adsorption on Fh significantly surpassed that of TiO<sub>2</sub> within pH 3.0 ∼ 8.0. The results of FTIR, XPS, and charge distribution multi-site complexation (CD-MUSIC) model testified that Cr(VI) retention on Fh occurred through monodentate and bidentate inner-sphere adsorption, whereas the inner- and outer-sphere complexes were formed on TiO<sub>2</sub>. ClO<sub>4</sub><sup>-</sup>, HCO<sub>3</sub><sup>-</sup>, and SO<sub>4</sub><sup>2-</sup>, which mostly undergo outer-sphere adsorption, could barely interfere with Cr(VI) adsorption on Fh, but the outer-sphere adsorption of Cr(VI) on TiO<sub>2</sub> was greatly inhibited. By creating a cation-bridged surface complex, Cd<sup>2+</sup> significantly improved the adsorption and immobilization stability of Cr(VI), whereas Fe<sup>3+</sup> competed with Cr(VI) for the surface sites. According to desorption experiments, the Cr(VI) bidentate species was less likely to redissolve than the monodentate and outer-sphere complexes. Arsenate severely inhibited Cr(VI) adsorption but increased the percentage of monodentate adsorption of Cr(VI) on Fh, which in turn improved the mobility of Cr(VI). Our findings provide molecular-level surface structures and predictive models for Cr(VI) removal in the presence of coexisting ions, which is important in the co-removal of multiple pollutants.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"41 ","pages":"Article 104710"},"PeriodicalIF":7.1,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The synergistic performance of redox couples enhanced with phase inter-grown ceria-zirconia-alumina mixed oxides: A robust three-way catalyst for diesel exhaust abatement 相间生长的铈-锆-氧化铝混合氧化物增强氧化还原对的协同性能：一种用于柴油废气减排的强大三向催化剂

IF 7.1 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-12-17 DOI: 10.1016/j.eti.2025.104708

Varuna Jayachandran , Priyadharsini Natesan , Balaji Chettiannan , Jet-Chau Wen , Talat Ali , Mohd Shkir , Elango Muniappan , Sambasivam Sangaraju

The CeO₂-ZrO₂ and Al₂O₃ (75 wt% and 25 wt%) were prepared by co-precipitation route, calcined at 500 °C for 5 h and mechanically mixed. Three compositions of Ce/Zr (20:80, 50:50, and 80:20) were synthesized and their microstructural properties was observed via X-ray diffraction (XRD), and Raman analysis. The CeO₂-ZrO₂/Al₂O₃ (Ce/Zr-50:50) sample reveals the mixed phase formation of cubic and tetragonal, which facilitates the catalytic activity via oxygen ion diffusion from O^2- sub-lattice to the sample surface. The CeO₂-ZrO₂/Al₂O₃ (Ce/Zr-50:50) was further investigated using High-resolution transmission electron microscope (HRTEM), Brunauer-Emmett-Teller (BET) study, X-ray photoelectron spectroscopy (XPS), and Scanning electron microscope (SEM). The surface area of CeO₂-ZrO₂/Al₂O₃ (Ce/Zr −50:50) was 91.2 m² g⁻¹, which provides surface active oxygen species to participate in diesel exhaust treatment via redox reactions. The XPS spectra of CeO₂-ZrO₂/Al₂O₃ (Ce/Zr −50:50) sample confirms the Ce³⁺ reduction along with the formation of oxygen vacancies. To perform catalytic reaction, the CeO₂-ZrO₂/Al₂O₃ (Ce/Zr −50:50) sample was coated on the perforated stainless-steel substrates by a unique and cost-effective technique, which could be economically advantageous to produce the catalyst for commercial uses on a large scale. The sample was tested at different load conditions. At maximum load condition, the sample exhibited substantial reduction in CO, HC, CO₂, NO_x, and smoke.

采用共沉淀法制备CeO2-ZrO2和Al2O3(75 wt%和25 wt%)，在500℃下煅烧5 h，机械混合。合成了三种Ce/Zr（20:80, 50:50和80:20）的成分，并通过x射线衍射（XRD）和拉曼分析观察了它们的微观结构特性。CeO2-ZrO2/Al2O3 （Ce/Zr-50:50）样品显示出立方和四方混合相的形成，这有利于氧离子从O2-亚晶格扩散到样品表面的催化活性。采用高分辨率透射电子显微镜（HRTEM）、布鲁诺尔-埃米特-泰勒（BET）、x射线光电子能谱（XPS）和扫描电子显微镜（SEM）对CeO2-ZrO2/Al2O3 （Ce/Zr-50:50）进行了进一步的研究。CeO2-ZrO2/Al2O3 （Ce/Zr−50:50）的表面积为91.2 m2 g−1，为柴油机尾气的氧化还原处理提供了表面活性氧。CeO2-ZrO2/Al2O3 （Ce/Zr−50:50）样品的XPS光谱证实了Ce3+的还原以及氧空位的形成。为了进行催化反应，将CeO2-ZrO2/Al2O3 （Ce/Zr−50:50）样品通过一种独特且经济高效的技术涂覆在多孔不锈钢衬底上，可以在经济上大规模生产用于商业用途的催化剂。对试样进行了不同载荷条件下的试验。在最大负载条件下，样品中CO、HC、CO2、NOx和烟雾的含量显著降低。

{"title":"The synergistic performance of redox couples enhanced with phase inter-grown ceria-zirconia-alumina mixed oxides: A robust three-way catalyst for diesel exhaust abatement","authors":"Varuna Jayachandran , Priyadharsini Natesan , Balaji Chettiannan , Jet-Chau Wen , Talat Ali , Mohd Shkir , Elango Muniappan , Sambasivam Sangaraju","doi":"10.1016/j.eti.2025.104708","DOIUrl":"10.1016/j.eti.2025.104708","url":null,"abstract":"<div><div>The CeO<sub>2</sub>-ZrO<sub>2</sub> and Al<sub>2</sub>O<sub>3</sub> (75 wt% and 25 wt%) were prepared by co-precipitation route, calcined at 500 °C for 5 h and mechanically mixed. Three compositions of Ce/Zr (20:80, 50:50, and 80:20) were synthesized and their microstructural properties was observed via X-ray diffraction (XRD), and Raman analysis. The CeO<sub>2</sub>-ZrO<sub>2</sub>/Al<sub>2</sub>O<sub>3</sub> (Ce/Zr-50:50) sample reveals the mixed phase formation of cubic and tetragonal, which facilitates the catalytic activity via oxygen ion diffusion from O<sup>2-</sup> sub-lattice to the sample surface. The CeO<sub>2</sub>-ZrO<sub>2</sub>/Al<sub>2</sub>O<sub>3</sub> (Ce/Zr-50:50) was further investigated using High-resolution transmission electron microscope (HRTEM), Brunauer-Emmett-Teller (BET) study, X-ray photoelectron spectroscopy (XPS), and Scanning electron microscope (SEM). The surface area of CeO<sub>2</sub>-ZrO<sub>2</sub>/Al<sub>2</sub>O<sub>3</sub> (Ce/Zr −50:50) was 91.2 m<sup>2</sup> g<sup>−1</sup>, which provides surface active oxygen species to participate in diesel exhaust treatment via redox reactions. The XPS spectra of CeO<sub>2</sub>-ZrO<sub>2</sub>/Al<sub>2</sub>O<sub>3</sub> (Ce/Zr −50:50) sample confirms the Ce<sup>3+</sup> reduction along with the formation of oxygen vacancies. To perform catalytic reaction, the CeO<sub>2</sub>-ZrO<sub>2</sub>/Al<sub>2</sub>O<sub>3</sub> (Ce/Zr −50:50) sample was coated on the perforated stainless-steel substrates by a unique and cost-effective technique, which could be economically advantageous to produce the catalyst for commercial uses on a large scale. The sample was tested at different load conditions. At maximum load condition, the sample exhibited substantial reduction in CO, HC, CO<sub>2</sub>, NO<sub>x</sub>, and smoke.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"41 ","pages":"Article 104708"},"PeriodicalIF":7.1,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two-stage inoculation of partially antagonistic Bacillus sp. strains improves imidacloprid pesticide degradation and humic acid formation during vegetable waste composting 两阶段接种部分拮抗芽孢杆菌提高了蔬菜废弃物堆肥过程中吡虫啉农药的降解和腐植酸的形成

IF 7.1 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-12-15 DOI: 10.1016/j.eti.2025.104704

Yajun Duan , Guohui Ning , Weizhe Li , Xue Wang , Jiahui Li , Mingyue Qi , Xiaomin Wang , Yali Huang , Jianfeng Xu , Cunpeng Zhao , Zhixin Yang , Yubo Wang

While phased inoculation has been demonstrated to accelerate compost maturation, its potential effects on detoxifying pesticide-laden vegetable waste remains to be demonstrated. The objective of this study was to determine the efficacy of two-stage inoculation on composting imidacloprid (IMI)-spiked vegetable waste, employing four lignocellulose-degrading Bacillus strains from our strain collection. In IMI-containing mineral salt medium, B. stratosphericus strain M1, B. licheniformis strain Q1, B. subtilis strain QK, and B. altitudinis strain F11 were all individually capable to degrade the pesticide, in decreasing order, with removal efficiencies between 51 % and 10 %. A combined mixture M, used as a first inoculum for composting, excluded strain F11, due to antagonistic growth effects. After 7 days of composting, a secondary inoculation with strain F11 was applied. This two-stage inoculation (MF) enhanced IMI degradation compared to the non-inoculated control and single-stage inoculation with M, reducing IMI half-life by 8.7 days and 1.5 days, respectively. When composting was complete, MF had achieved 81 % IMI degradation, with exogenous bacteria contributing 77 % of the removal efficiency, 63 % higher than the M treatment. Additionally, MF increased the final humic acid content and polymerization degree by 17 % and 25 %, respectively, compared to M treatment, while reducing the fulvic acid content. Multiple linear regression confirmed that fulvic acid content and IMI residue concentrations were significant factors affecting humic acid content in inoculated treatments. This study shows that two-stage inoculation can provide dual benefits of accelerating vegetable waste composting and detoxifying pesticide residues, offering an efficient strategy for sustainable agricultural waste management.

虽然分阶段接种已被证明可以加速堆肥的成熟，但其对含有农药的蔬菜废物解毒的潜在影响仍有待证明。本研究的目的是利用我们收集的4株木质纤维素降解芽孢杆菌菌株，确定两阶段接种对吡虫啉（IMI）添加蔬菜废弃物堆肥的效果。在含imi无机盐培养基中，平流层芽孢杆菌M1、地衣芽孢杆菌Q1、枯草芽孢杆菌QK、高原芽孢杆菌F11对农药的降解效率依次递减，去除率在51 % ~ 10 %之间。组合混合物M作为堆肥的第一接种物，由于拮抗生长作用，将菌株F11排除在外。堆肥7 d后，再次接种菌株F11。与未接种对照和单阶段接种M相比，两阶段接种（MF）增强了IMI的降解，IMI半衰期分别缩短了8.7天和1.5天。堆肥完成后，MF对IMI的降解率达到81 %，其中外源细菌的去除效率为77 %，比M处理高63 %。此外，与M处理相比，MF处理使最终腐植酸含量和聚合度分别提高了17 %和25 %，同时降低了黄腐酸含量。多元线性回归证实，黄腐酸含量和IMI残留浓度是影响接种处理腐植酸含量的显著因素。本研究表明，两阶段接种具有加速蔬菜废弃物堆肥和去除农药残留的双重效益，为农业废弃物的可持续管理提供了有效的策略。

{"title":"Two-stage inoculation of partially antagonistic Bacillus sp. strains improves imidacloprid pesticide degradation and humic acid formation during vegetable waste composting","authors":"Yajun Duan , Guohui Ning , Weizhe Li , Xue Wang , Jiahui Li , Mingyue Qi , Xiaomin Wang , Yali Huang , Jianfeng Xu , Cunpeng Zhao , Zhixin Yang , Yubo Wang","doi":"10.1016/j.eti.2025.104704","DOIUrl":"10.1016/j.eti.2025.104704","url":null,"abstract":"<div><div>While phased inoculation has been demonstrated to accelerate compost maturation, its potential effects on detoxifying pesticide-laden vegetable waste remains to be demonstrated. The objective of this study was to determine the efficacy of two-stage inoculation on composting imidacloprid (IMI)-spiked vegetable waste, employing four lignocellulose-degrading <em>Bacillus</em> strains from our strain collection. In IMI-containing mineral salt medium, <em>B. stratosphericus</em> strain M1, <em>B. licheniformis</em> strain Q1, <em>B. subtilis</em> strain QK, and <em>B. altitudinis</em> strain F11 were all individually capable to degrade the pesticide, in decreasing order, with removal efficiencies between 51 % and 10 %. A combined mixture M, used as a first inoculum for composting, excluded strain F11, due to antagonistic growth effects. After 7 days of composting, a secondary inoculation with strain F11 was applied. This two-stage inoculation (MF) enhanced IMI degradation compared to the non-inoculated control and single-stage inoculation with M, reducing IMI half-life by 8.7 days and 1.5 days, respectively. When composting was complete, MF had achieved 81 % IMI degradation, with exogenous bacteria contributing 77 % of the removal efficiency, 63 % higher than the M treatment. Additionally, MF increased the final humic acid content and polymerization degree by 17 % and 25 %, respectively, compared to M treatment, while reducing the fulvic acid content. Multiple linear regression confirmed that fulvic acid content and IMI residue concentrations were significant factors affecting humic acid content in inoculated treatments. This study shows that two-stage inoculation can provide dual benefits of accelerating vegetable waste composting and detoxifying pesticide residues, offering an efficient strategy for sustainable agricultural waste management.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"41 ","pages":"Article 104704"},"PeriodicalIF":7.1,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilizing industrial waste for preparation of rGO-like carbon /TiO2 composites derived from cassava residue for counter electrodes in dye-sensitized solar cells 利用工业废料制备由木薯渣衍生的rgo样碳/TiO2复合材料，用于染料敏化太阳能电池的对电极

IF 7.1 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-12-15 DOI: 10.1016/j.eti.2025.104703

Woranuch Sudthongkong , Sudarat Premsiripat , Nattakan Kanjana , Akkawat Ruammaitree , Orapan Saensuk , Poramed Wongjom , Yingyot Infahsaeng , Wasan Maiaugree

Industries utilizing cassava-derived products generate significant waste during production, including cassava residue (CR), yeast-fermented cassava residue (YCR), cassava peels (CPs), and yeast-fermented cassava peels (YCPs). Reduced graphene oxide (rGO) was synthesized from these wastes to utilize this biomass while creating value-added materials, using a modified Hummers' method followed by reduction under nitrogen gas at 700 °C. rGO-like carbon was mixed with titanium dioxide (TiO₂) to enhance its properties and subsequently coated onto FTO glass substrates using a doctor blade. This composite material was specifically developed to serve as a counter electrode (CE) in dye-sensitized solar cells (DSSCs), providing a sustainable approach by valorizing cassava biomass for renewable energy devices. The DSSCs of champion cell based on rGO-like carbon/TiO₂ composite derived from cassava peels achieved a power conversion efficiency (PCE) of 6.86 %, with an open-circuit voltage (V_oc) of 0.76 V, a short-circuit current density (J_sc) of 15.80 mA/cm², and a fill factor (FF) of 0.57. The solar cell efficiency of rGO-like carbon/TiO₂ CE is comparable to that of a platinum (Pt) based device (6.68 %).

利用木薯衍生产品的工业在生产过程中产生大量废物，包括木薯渣（CR）、酵母发酵木薯渣（YCR）、木薯皮（CPs）和酵母发酵木薯皮（YCPs）。利用这些废物合成还原性氧化石墨烯（rGO），同时利用这些生物质创造增值材料，使用改进的Hummers方法，然后在700℃的氮气下还原。rGO类碳与二氧化钛（TiO2）混合以增强其性能，随后使用医生刀涂覆在FTO玻璃基板上。这种复合材料专门用于染料敏化太阳能电池（DSSCs）的对电极（CE），为可再生能源设备提供了一种可持续的方法，使木薯生物质增值。以木薯皮为原料制备的rgo样碳/TiO2复合材料制备的DSSCs的功率转换效率（PCE）为6.86 %，开路电压（Voc）为0.76 V，短路电流密度（Jsc）为15.80 mA/cm²，填充因子（FF）为0.57。rgo类碳/TiO2 CE的太阳能电池效率与铂基器件相当（6.68 %）。

{"title":"Utilizing industrial waste for preparation of rGO-like carbon /TiO2 composites derived from cassava residue for counter electrodes in dye-sensitized solar cells","authors":"Woranuch Sudthongkong , Sudarat Premsiripat , Nattakan Kanjana , Akkawat Ruammaitree , Orapan Saensuk , Poramed Wongjom , Yingyot Infahsaeng , Wasan Maiaugree","doi":"10.1016/j.eti.2025.104703","DOIUrl":"10.1016/j.eti.2025.104703","url":null,"abstract":"<div><div>Industries utilizing cassava-derived products generate significant waste during production, including cassava residue (CR), yeast-fermented cassava residue (YCR), cassava peels (CPs), and yeast-fermented cassava peels (YCPs). Reduced graphene oxide (rGO) was synthesized from these wastes to utilize this biomass while creating value-added materials, using a modified Hummers' method followed by reduction under nitrogen gas at 700 °C. rGO-like carbon was mixed with titanium dioxide (TiO<sub>2</sub>) to enhance its properties and subsequently coated onto FTO glass substrates using a doctor blade. This composite material was specifically developed to serve as a counter electrode (CE) in dye-sensitized solar cells (DSSCs), providing a sustainable approach by valorizing cassava biomass for renewable energy devices. The DSSCs of champion cell based on rGO-like carbon/TiO<sub>2</sub> composite derived from cassava peels achieved a power conversion efficiency (PCE) of 6.86 %, with an open-circuit voltage (<em>V</em><sub><em>oc</em></sub>) of 0.76 V, a short-circuit current density (<em>J</em><sub><em>sc</em></sub>) of 15.80 mA/cm², and a fill factor (FF) of 0.57. The solar cell efficiency of rGO-like carbon/TiO<sub>2</sub> CE is comparable to that of a platinum (Pt) based device (6.68 %).</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"41 ","pages":"Article 104703"},"PeriodicalIF":7.1,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contrasting patterns of antibiotic resistance and metal resistance genes between free living and particle attached bacteria in a hypertrophic lake 富营养化湖泊中自由生活细菌和颗粒附着细菌对抗生素和金属抗性基因的对比分析

IF 7.1 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-12-15 DOI: 10.1016/j.eti.2025.104705

Shengxing Wang , Yiwei Lu , Lei Jiang , Kaining Chen , Jiawei Gao , Shiqiang Wan , Li-Jun Zhou , Qinglong L. Wu

The eutrophication and algal blooms caused by human activities significantly change the structure of lake microbial communities. Yet whether this process can also cause changes in the dissemination of antibiotic resistance genes (ARGs), metal resistance genes (MRGs), and mobile genetic elements (MGEs) urgently needs to be investigated. In this study, metagenomic approaches were employed to investigate the abundance, diversity, relationships and hosts of ARGs, MRGs, MGEs and virulence factors (VFs) in free-living (FL) and particle-associated (PA) fractions during cyanobacterial blooms. The diversities of resistance genes were significantly higher in the FL fraction, while the abundance of MRGs and MGEs were greater in the PA fraction. Large-sized cyanobacterial aggregates may alter the bacterial community structure and modulate the diversity and abundance of resistance genes across distinct fractions. These aggregates enhanced topological complexity and multidimensional connectivity of microbial networks, while harboring elevated transposon abundance. Metagenomic assembly results demonstrated a high-frequency co-occurrence of transposons with MRGs, particularly in cyanobacteria-derived assembled sequences. This suggests that cyanobacterial aggregates, which are enriched with transposases, may facilitate the dissemination of MRGs in the environment. Furthermore, we identified potential pathogenic bacteria harboring multiple resistance genes and VFs. This work provides a systematic profile of resistance genes across fractions during cyanobacterial blooms, revealing distinct trends between ARGs and MRGs. These findings offer critical insights for developing effective lake management strategies to curb the dissemination of resistance genes.

人类活动引起的富营养化和藻华显著改变了湖泊微生物群落结构。然而，这一过程是否也会引起抗生素耐药基因（ARGs）、金属耐药基因（MRGs）和移动遗传元件（MGEs）传播的变化，迫切需要研究。在本研究中，采用宏基因组方法研究了蓝藻华期间自由生活（FL）和颗粒相关（PA）组分中ARGs、MRGs、MGEs和毒力因子（VFs）的丰度、多样性、关系和宿主。耐药基因多样性在FL组分中较高，而MRGs和MGEs丰度在PA组分中较高。大型蓝藻聚集体可能改变细菌群落结构，调节不同组分间抗性基因的多样性和丰度。这些聚集体增强了微生物网络的拓扑复杂性和多维连通性，同时拥有升高的转座子丰度。宏基因组组装结果表明，转座子与MRGs高频共存，特别是在蓝藻衍生的组装序列中。这表明富含转座酶的蓝藻聚集体可能促进MRGs在环境中的传播。此外，我们还发现了含有多种耐药基因和VFs的潜在致病菌。这项工作提供了在蓝藻华期间的抗性基因的系统概况，揭示了ARGs和MRGs之间的不同趋势。这些发现为制定有效的湖泊管理策略以遏制抗性基因的传播提供了重要的见解。

{"title":"Contrasting patterns of antibiotic resistance and metal resistance genes between free living and particle attached bacteria in a hypertrophic lake","authors":"Shengxing Wang , Yiwei Lu , Lei Jiang , Kaining Chen , Jiawei Gao , Shiqiang Wan , Li-Jun Zhou , Qinglong L. Wu","doi":"10.1016/j.eti.2025.104705","DOIUrl":"10.1016/j.eti.2025.104705","url":null,"abstract":"<div><div>The eutrophication and algal blooms caused by human activities significantly change the structure of lake microbial communities. Yet whether this process can also cause changes in the dissemination of antibiotic resistance genes (ARGs), metal resistance genes (MRGs), and mobile genetic elements (MGEs) urgently needs to be investigated. In this study, metagenomic approaches were employed to investigate the abundance, diversity, relationships and hosts of ARGs, MRGs, MGEs and virulence factors (VFs) in free-living (FL) and particle-associated (PA) fractions during cyanobacterial blooms. The diversities of resistance genes were significantly higher in the FL fraction, while the abundance of MRGs and MGEs were greater in the PA fraction. Large-sized cyanobacterial aggregates may alter the bacterial community structure and modulate the diversity and abundance of resistance genes across distinct fractions. These aggregates enhanced topological complexity and multidimensional connectivity of microbial networks, while harboring elevated transposon abundance. Metagenomic assembly results demonstrated a high-frequency co-occurrence of transposons with MRGs, particularly in cyanobacteria-derived assembled sequences. This suggests that cyanobacterial aggregates, which are enriched with transposases, may facilitate the dissemination of MRGs in the environment. Furthermore, we identified potential pathogenic bacteria harboring multiple resistance genes and VFs. This work provides a systematic profile of resistance genes across fractions during cyanobacterial blooms, revealing distinct trends between ARGs and MRGs. These findings offer critical insights for developing effective lake management strategies to curb the dissemination of resistance genes.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"41 ","pages":"Article 104705"},"PeriodicalIF":7.1,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel strategy for the management of drug-resistant bacteria in wastewater: Preparation and application of bacteriophage-loaded functionalized Fe3O4 nanoparticles 管理废水中耐药细菌的新策略：噬菌体负载功能化Fe3O4纳米颗粒的制备和应用

IF 7.1 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-12-15 DOI: 10.1016/j.eti.2025.104702

Pei Wang , Xiangyu Kong , Qibai Chen , Shenyu Pang , Shunyuan Pan , Zepeng Zhao , He Li , Dongyang Gao , Jun Song

Water resource contamination by drug-resistant bacteria is a critical issue, which directly increases the complexity and difficulty of wastewater treatment. Given the limitations of conventional wastewater treatment methodologies in addressing drug-resistant bacterial contamination, there is an imperative need for the development of novel wastewater treatment technologies. In this study, magnetic Fe₃O₄ nanoparticles was selected as a bacteriophage (phage) delivery system to improve the bacterial capture efficiency of phages. The specific recognition ability of phages and the functional characteristics of Fe₃O₄ were used to achieve efficient targeted removal of drug-resistant bacteria in wastewater through a synergistic effect. The magnetism of the system makes it highly recyclable, minimizes the use of chemical reagents, and reduces the risk of secondary pollution. The results demonstrated that magnetic Fe₃O₄ nanoparticles exhibit good magnetism and can load phages from various host genera as well as different phage families. Surfactin can effectively improve the dispersion and loading capacity of Fe₃O₄. The highest loading efficiency is achieved within 2 h. The loading amount of phage GEP101 reached 2.44 × 10⁸ PFU/mL, with a loading rate of 85.12 %. The loading rate of other phages can exceed 75 %. Within 3 h, it removed 96.82 % of Escherichia coli, significantly inhibited biofilm formation, and maintained a recovery rate of 84.67 % and a loading rate of 53.71 % after 6 cycles. In simulated wastewater, its sterilization rate (97.39 %) was significantly higher than that of other groups, offering a novel solution to the contamination of wastewater by drug-resistant bacteria.

耐药细菌污染水资源是一个关键问题，它直接增加了废水处理的复杂性和难度。鉴于传统废水处理方法在处理耐药细菌污染方面的局限性，迫切需要开发新的废水处理技术。本研究选择磁性Fe3O4纳米颗粒作为噬菌体（phage）递送系统，以提高噬菌体的细菌捕获效率。利用噬菌体的特异性识别能力和Fe3O4的功能特性，通过协同作用，实现对废水中耐药菌的高效靶向去除。该系统的磁性使其高度可回收，最大限度地减少了化学试剂的使用，并降低了二次污染的风险。结果表明，磁性Fe3O4纳米颗粒具有良好的磁性，可以装载来自不同宿主属和不同噬菌体家族的噬菌体。表面素能有效提高Fe3O4的分散和负载能力。在2 h内达到最高加载效率。噬菌体GEP101的载药量为2.44 × 108 PFU/mL，载药率为85.12 %。其他噬菌体的加载率可超过75% %。在3 h内，去除大肠杆菌96.82 %，显著抑制生物膜的形成，6次循环后，回收率为84.67 %，加载率为53.71 %。在模拟废水中，其灭菌率（97.39 %）显著高于其他组，为耐药菌污染废水提供了新的解决方案。

{"title":"A novel strategy for the management of drug-resistant bacteria in wastewater: Preparation and application of bacteriophage-loaded functionalized Fe3O4 nanoparticles","authors":"Pei Wang , Xiangyu Kong , Qibai Chen , Shenyu Pang , Shunyuan Pan , Zepeng Zhao , He Li , Dongyang Gao , Jun Song","doi":"10.1016/j.eti.2025.104702","DOIUrl":"10.1016/j.eti.2025.104702","url":null,"abstract":"<div><div>Water resource contamination by drug-resistant bacteria is a critical issue, which directly increases the complexity and difficulty of wastewater treatment. Given the limitations of conventional wastewater treatment methodologies in addressing drug-resistant bacterial contamination, there is an imperative need for the development of novel wastewater treatment technologies. In this study, magnetic Fe<sub>3</sub>O<sub>4</sub> nanoparticles was selected as a bacteriophage (phage) delivery system to improve the bacterial capture efficiency of phages. The specific recognition ability of phages and the functional characteristics of Fe<sub>3</sub>O<sub>4</sub> were used to achieve efficient targeted removal of drug-resistant bacteria in wastewater through a synergistic effect. The magnetism of the system makes it highly recyclable, minimizes the use of chemical reagents, and reduces the risk of secondary pollution. The results demonstrated that magnetic Fe<sub>3</sub>O<sub>4</sub> nanoparticles exhibit good magnetism and can load phages from various host genera as well as different phage families. Surfactin can effectively improve the dispersion and loading capacity of Fe<sub>3</sub>O<sub>4</sub>. The highest loading efficiency is achieved within 2 h. The loading amount of phage GEP101 reached 2.44 × 10<sup>8</sup> PFU/mL, with a loading rate of 85.12 %. The loading rate of other phages can exceed 75 %. Within 3 h, it removed 96.82 % of <em>Escherichia coli</em>, significantly inhibited biofilm formation, and maintained a recovery rate of 84.67 % and a loading rate of 53.71 % after 6 cycles. In simulated wastewater, its sterilization rate (97.39 %) was significantly higher than that of other groups, offering a novel solution to the contamination of wastewater by drug-resistant bacteria.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"41 ","pages":"Article 104702"},"PeriodicalIF":7.1,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-step synthesis of hierarchical porous Fe-supported biochar for rapid and efficient removal of antibiotics from water 一步法合成分级多孔铁负载生物炭用于快速高效去除水中抗生素

IF 7.1 2区环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Environmental Technology & Innovation

Pub Date : 2025-12-15 DOI: 10.1016/j.eti.2025.104699

Jun Dong , Yuanjun Tang , Jiayin Ma , Peikun Jiang , Yinxiu Liu , Ruohui Lu , Yin Li , Shuqi Li , Yangqing Hu , Fei Wang

The widespread use of antibiotics has led to their accumulation in water, posing serious environmental risks. Biochar has emerged as a promising adsorbent for antibiotic removal, but its performance is often limited by low capacity and slow kinetics, especially for high-molecular-weight antibiotics. In this study, a hierarchical porous Fe-supported biochar (HPFeBC) was synthesized via one-step pyrolysis of pine sawdust using Fe(NO₃)₃ as both activator and template. The obtained HPFeBC exhibited a well-interconnected pore structure with a high surface area (417.23 m²/g) and enhanced mesoporosity, significantly improving mass transfer and accessibility of adsorption sites. HPFeBC demonstrated exceptional adsorption performance for both low-molecular-weight antibiotic tetracycline (TC) and high-molecular-weight antibiotic tylosin (TYL), with maximum capacities of 361.23 mg/g and 173.61 mg/g, respectively. Notably, HPFeBC achieved rapid adsorption equilibrium within 5 mins for TC and 30 mins for TYL, far surpassing commercial adsorbents. The adsorption process followed the pseudo-second-order kinetics and the Freundlich isotherm model, and thermodynamic analysis confirmed the spontaneous and endothermic nature of adsorption. The adsorption mechanism involved pore filling, π-π interactions, hydrogen bonding, surface complexation, and limited redox reaction. HPFeBC also exhibited excellent stability over a wide pH range, strong anti-interference ability, and good reusability. Overall, the synthesis HPFeBC can act as an effective candidate for rapid and efficient removal of antibiotics from water.

抗生素的广泛使用导致其在水中积聚，构成严重的环境风险。生物炭已成为一种很有前途的抗生素去除吸附剂，但其性能往往受到低容量和慢动力学的限制，特别是对于高分子量抗生素。在本研究中，以Fe(NO3)3为活化剂和模板剂，通过一步热解法合成了层次化多孔Fe负载生物炭（HPFeBC）。制备的HPFeBC具有良好的孔隙结构，具有较高的比表面积（417.23 m2/g）和介孔率，显著改善了传质和吸附位点的可及性。HPFeBC对低分子量抗生素四环素（TC）和高分子量抗生素泰络素（tylosin）均表现出优异的吸附性能，最大吸附量分别为361.23 mg/g和173.61 mg/g。值得注意的是，HPFeBC在5 min内达到了TC的快速吸附平衡，在30 min内达到了TYL的快速吸附平衡，远远超过了商用吸附剂。吸附过程符合拟二级动力学和Freundlich等温模型，热力学分析证实了吸附的自发和吸热性质。吸附机理包括孔隙填充、π-π相互作用、氢键、表面络合和有限氧化还原反应。HPFeBC在较宽的pH范围内具有良好的稳定性，抗干扰能力强，可重复使用。综上所述，合成的HPFeBC可作为快速高效去除水中抗生素的有效候选物。

{"title":"One-step synthesis of hierarchical porous Fe-supported biochar for rapid and efficient removal of antibiotics from water","authors":"Jun Dong , Yuanjun Tang , Jiayin Ma , Peikun Jiang , Yinxiu Liu , Ruohui Lu , Yin Li , Shuqi Li , Yangqing Hu , Fei Wang","doi":"10.1016/j.eti.2025.104699","DOIUrl":"10.1016/j.eti.2025.104699","url":null,"abstract":"<div><div>The widespread use of antibiotics has led to their accumulation in water, posing serious environmental risks. Biochar has emerged as a promising adsorbent for antibiotic removal, but its performance is often limited by low capacity and slow kinetics, especially for high-molecular-weight antibiotics. In this study, a hierarchical porous Fe-supported biochar (HPFeBC) was synthesized via one-step pyrolysis of pine sawdust using Fe(NO<sub>3</sub>)<sub>3</sub> as both activator and template. The obtained HPFeBC exhibited a well-interconnected pore structure with a high surface area (417.23 m<sup>2</sup>/g) and enhanced mesoporosity, significantly improving mass transfer and accessibility of adsorption sites. HPFeBC demonstrated exceptional adsorption performance for both low-molecular-weight antibiotic tetracycline (TC) and high-molecular-weight antibiotic tylosin (TYL), with maximum capacities of 361.23 mg/g and 173.61 mg/g, respectively. Notably, HPFeBC achieved rapid adsorption equilibrium within 5 mins for TC and 30 mins for TYL, far surpassing commercial adsorbents. The adsorption process followed the pseudo-second-order kinetics and the Freundlich isotherm model, and thermodynamic analysis confirmed the spontaneous and endothermic nature of adsorption. The adsorption mechanism involved pore filling, π-π interactions, hydrogen bonding, surface complexation, and limited redox reaction. HPFeBC also exhibited excellent stability over a wide pH range, strong anti-interference ability, and good reusability. Overall, the synthesis HPFeBC can act as an effective candidate for rapid and efficient removal of antibiotics from water.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"41 ","pages":"Article 104699"},"PeriodicalIF":7.1,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0