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Organic substitution contrasting direct fertilizer reduction increases wheat productivity, soil quality, microbial diversity and network complexity 有机替代与直接减少化肥形成鲜明对比,提高了小麦产量、土壤质量、微生物多样性和网络复杂性
IF 6.7 2区 环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-05 DOI: 10.1016/j.eti.2024.103784
Hao He , Mengwen Peng , Zhenan Hou , Junhua Li

Excessive use of chemical fertilizers negatively impacts crop productivity and farmland ecosystem, impeding sustainable agricultural progress. Consequently, there is an immediate need for a chemical fertilizer reduction strategy that ensures crop productivity and improves soil quality and the ecological environment of farmland. This study implemented a three-year (2018–2020) field experiment with two chemical fertilizer reduction methods (direct fertilizer reduction and organic substitution) to investigate their effects on wheat productivity, soil quality, heavy metal pollution risk and microbial characteristics. The results showed that organic substitution treatments (OF1, OF2 and OF3) improved most wheat plant (nutrient uptake and yield and its components) and soil properties (soil nutrients and carbon and nitrogen fractions), leading to increased crop productivity index (CPI, by 9.18 %-16.39 % and 14.14 %-23.36 %) and soil quality index (SQI, by 84.67 %-138.86 % and 104.11 %-175.91 %) compared to conventional fertilization (CF) and direct fertilizer reduction treatments (RF1, RF2 and RF3) in 2019 and 2020. Additionally, organic substitution enhanced the diversity and network complexity of bacterial community, while raising the soil pollution index (SPI, by 9.30 %-12.84 % and 12.20 %-18.49 %) without causing soil heavy metal pollution. Thus, it is recommended to adopt organic fertilizer substitution as the primary chemical fertilizer reduction strategy for wheat production. This approach will ensure crop yield, and improve soil quality and microbial characteristics, but its long-term application requires monitoring changes in soil heavy metals. Overall, this study provides guidelines for implementing scientific fertilization in agricultural practices, thus contributing to the health and sustainability of farmland ecosystems.

化肥的过度使用对作物生产力和农田生态系统产生了负面影响,阻碍了农业的可持续发展。因此,迫切需要一种既能保证作物产量,又能改善土壤质量和农田生态环境的化肥减量策略。本研究实施了为期三年(2018-2020 年)的田间试验,采用两种化肥减量方法(化肥直接减量和有机替代),研究其对小麦产量、土壤质量、重金属污染风险和微生物特性的影响。结果表明,有机替代处理(OF1、OF2 和 OF3)改善了大部分小麦植株(养分吸收和产量及其组分)和土壤性质(土壤养分和碳氮组分),使作物生产力指数(CPI)提高了 9.与常规施肥(CF)和直接减肥处理(RF1、RF2 和 RF3)相比,2019 年和 2020 年的作物生产力指数(CPI,提高了 9.18 %-16.39 % 和 14.14 %-23.36 %)和土壤质量指数(SQI,提高了 84.67 %-138.86 % 和 104.11 %-175.91 %)均有所提高。此外,有机替代提高了细菌群落的多样性和网络复杂性,同时提高了土壤污染指数(SPI,分别为 9.30 %-12.84 % 和 12.20 %-18.49 %),但不会造成土壤重金属污染。因此,建议采用有机肥替代化肥作为小麦生产的主要化肥减量策略。这种方法既能保证作物产量,又能改善土壤质量和微生物特性,但长期应用需要监测土壤重金属的变化。总之,本研究为在农业实践中实施科学施肥提供了指导,从而有助于农田生态系统的健康和可持续发展。
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引用次数: 0
Transmembrane and transcriptome analysis reveals the mechanism of dietary supplements in reducing cadmium absorption and toxicity 跨膜和转录组分析揭示膳食补充剂减少镉吸收和毒性的机制
IF 6.7 2区 环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-03 DOI: 10.1016/j.eti.2024.103771
Shuo Sun , Ping Zhuang , Zhian Li , Hui Mo

Dietary strategies have proven to be effective in preventing cadmium (Cd) poisoning, but the regulation of the bioavailability (BAV) and transport of Cd in the body, as well as their molecular mechanisms remain unclear. In this study, the Caco-2 cell models were applied to investigate the effects of 15 dietary supplements on Cd bioavailability (Cd-BAV) in Cd-contaminated water through a transmembrane transport assay and the mechanisms of three selected supplements in inhibiting Cd toxicity and uptake. The results demonstrated that the Cd-BAV in water varied from 7.89 % to 18.4 % using simulated gastrointestinal digestion and cell model. The addition of MT, Zn, and OPCs significantly reduced Cd-BAV by 82.5 %, 73 % and 60.5 % and also strengthened transmembrane electrical resistance (TEER), indicating the protective effects of these compounds against Cd-induced barrier dysfunction. Moreover, the results of transcriptomics revealed that these components can modulate the expression of specific pathway genes associated with the toxicity and absorption of dietary Cd, such as the apoptosis pathway (Jun, Ddit3), antioxidant-related pathways (Cat, Hmox1), and mineral absorption pathway (Mt, Slc39a4). This study highlights the importance of potential dietary interventions in public health and suggests that developing more effective measures to promote safer food consumption would be beneficial.

事实证明,膳食策略可有效预防镉(Cd)中毒,但镉在体内的生物利用度(BAV)和转运的调节及其分子机制仍不清楚。本研究应用 Caco-2 细胞模型,通过跨膜转运试验研究了 15 种膳食补充剂对镉污染水中镉生物利用度(Cd-BAV)的影响,以及所选三种补充剂抑制镉毒性和吸收的机制。结果表明,通过模拟胃肠道消化和细胞模型,水中的镉-BAV 从 7.89 % 到 18.4 % 不等。添加 MT、Zn 和 OPCs 后,Cd-BAV 明显降低了 82.5%、73% 和 60.5%,跨膜电阻(TEER)也有所增强,这表明这些化合物对 Cd 引起的屏障功能障碍具有保护作用。此外,转录组学研究结果显示,这些成分可以调节与膳食镉的毒性和吸收相关的特定通路基因的表达,如细胞凋亡通路()、抗氧化相关通路()和矿物质吸收通路()。这项研究强调了潜在膳食干预在公共卫生中的重要性,并表明制定更有效的措施来促进更安全的食品消费将是有益的。
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引用次数: 0
Assessments and application of low-cost sensors to study indoor air quality in layer facilities 评估和应用低成本传感器研究层设施的室内空气质量
IF 6.7 2区 环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-03 DOI: 10.1016/j.eti.2024.103773
Rowshon Afroz , Xinyang Guo , Chu-Wen Cheng , Sohaib Omar , V.L. Carney , Martin J. Zuidhof , Ran Zhao

Indoor poultry facilities often experience poor air quality due to intensive farming and restricted ventilation. Monitoring the air quality in these barns is crucial considering the health of both the birds and producers. Advancements in sensor technologies have led to the development of low-cost sensors (LCS) that can continuously monitor air pollutants. Even though most poultry facilities in Canada are indoors due to harsh winter weather conditions, there is a lack of indoor air quality (IAQ) studies. This study aimed to evaluate the field performance of the LCS network in a table egg farm in Canada, where the sensors were designed specifically for operating in dusty poultry facilities continuously. The LCS monitored IQA parameters such as particulate matter (PM), carbon dioxide (CO2), relative humidity, and temperature in real-time. By implementing a correction factor, the sensor data resulted in an agreement range of 80 ± 20% with a reference instrument. The study observed that PM concentration exceeded several thousand μg/m3, with PM10 at 5.5 × 104 ± 2.2 × 104 and PM2.5 at 6.3 × 103 ± 2.3 × 103, which was found to be most affected by the chicken activity and light regime. The IAQ parameters also exhibited a complex intercorrelation with each other, as well as the outdoor temperature and the building ventilation rate. Sensors were able to make observations that were found only with research-grade instruments in previous studies. Overall, the study showcases the potential of the LCS network as an affordable solution for environmental monitoring in poultry facilities.

由于集约化养殖和通风受限,室内家禽设施的空气质量通常很差。考虑到禽类和生产者的健康,监测这些禽舍的空气质量至关重要。传感器技术的进步推动了可持续监测空气污染物的低成本传感器 (LCS) 的发展。尽管由于冬季气候条件恶劣,加拿大大多数家禽养殖设施都在室内,但却缺乏室内空气质量 (IAQ) 研究。本研究旨在评估 LCS 网络在加拿大一家餐桌蛋鸡养殖场的现场性能,该养殖场的传感器专为在多尘的家禽设施中连续运行而设计。LCS 实时监测微粒物质 (PM)、二氧化碳 (CO)、相对湿度和温度等 IQA 参数。通过使用校正因子,传感器数据与参考仪器的一致性范围为 80 ± 20%。研究发现,可吸入颗粒物的浓度超过几千克/米,其中可吸入颗粒物的浓度为 5.5 × 10 ± 2.2 × 10,可吸入颗粒物的浓度为 6.3 × 10 ± 2.3 × 10。室内空气质量参数之间以及与室外温度和建筑通风率之间也存在复杂的相互关系。传感器能够观测到以往研究中只有研究级仪器才能观测到的情况。总之,这项研究展示了 LCS 网络作为经济实惠的家禽设施环境监测解决方案的潜力。
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引用次数: 0
Enrichment characteristics of polycyclic aromatic hydrocarbons in coal gangue of the Huaibei Coalfield, China 中国淮北煤田煤矸石中多环芳烃的富集特征
IF 6.7 2区 环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-02 DOI: 10.1016/j.eti.2024.103769
Zheng Du , Xiuping Hong , Kang Yang , Zihan Wang , Yingying Zhang , Xin Wang , Lichao Zhang , Yongjie Zhu

Coal gangue, a by-product of coal mining, deteriorates and oxidizes, causing environmental pollution. Despite extensive research on polycyclic aromatic hydrocarbon (PAHs) pollution in soil, aerosols, and water, studies on PAHs in coal gangue remain limited. This study aimed to fill this research gap by analyzing gangue samples from three areas in Huaibei. Sixteen priority parent polycyclic aromatic hydrocarbons (16PAHs) and alkyl polycyclic aromatic hydrocarbons (aPAHs) in the samples were analyzed qualitatively and quantitatively via gas chromatography-triple quadrupole mass spectrometry. The results showed that PAHs existed naturally in coal gangue. The aPAHs concentration of multiple samples from the same area (587.88 ng/g, 2972.73 ng/g, and 13528.29 ng/g from Liuqiao, Suntan, and Tongting, respectively) was higher than the 16PAHs concentration (528.79 ng/g, 570.16 ng/g, and 2818.79 ng/g from Liuqiao, Suntan, and Tongting, respectively). Among the 39 samples, the aPAHs concentration after weathering was 7732.78 ng/g, which was higher than the value in the fresh state of 4765.43 ng/g. 16PAHs with low ring number were dominant in the fresh state, but aPAHs with high ring number were dominant after weathering. The diagnostic ratios revealed that traditional diagnostic ratios may confuse sources of PAHs and that gangue should be considered as a single class of source materials. Additionally, there was no significant difference in the ratio between the weathered and fresh state. TEQBaP analysis showed that there was a certain environmental risk in the area and that TEQBaP(weathered) > TEQBaP(fresh). Therefore, the pollution attributable to PAHs in coal gangue, especially weathered gangue, warrants attention.

煤矸石是煤炭开采的副产品,会变质和氧化,造成环境污染。尽管对土壤、气溶胶和水中的多环芳烃污染进行了广泛研究,但对煤矸石中多环芳烃的研究仍然有限。本研究旨在通过分析淮北三个地区的煤矸石样本来填补这一研究空白。通过气相色谱-三重四极杆质谱法对样品中的 16 种重点母体多环芳烃(16PAHs)和烷基多环芳烃(aPAHs)进行了定性和定量分析。结果表明,煤矸石中天然存在多环芳烃。同一地区多个样品的 aPAHs 浓度(刘桥、孙潭、桐亭分别为 587.88 ng/g、2972.73 ng/g 和 13528.29 ng/g)高于 16PAHs 浓度(刘桥、孙潭、桐亭分别为 528.79 ng/g、570.16 ng/g 和 2818.79 ng/g)。在 39 个样品中,风化后的 aPAHs 浓度为 7732.78 ng/g,高于新鲜状态下的 4765.43 ng/g。16PAHs 在新鲜状态下以低环数为主,而风化后则以高环数为主。诊断比率表明,传统的诊断比率可能会混淆多环芳烃的来源,应将煤矸石视为一类单一的来源物质。此外,风化状态和新鲜状态下的比率没有显著差异。TEQ 分析表明,该地区存在一定的环境风险,且 TEQ(风化)> TEQ(新鲜)。因此,煤矸石,尤其是风化煤矸石中的多环芳烃造成的污染值得关注。
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引用次数: 0
Valorisation of agricultural residues into Thauera sp. Sel9 microbial proteins for aquaculture 将农业残留物转化为用于水产养殖的 Thauera sp.
IF 6.7 2区 环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-07-30 DOI: 10.1016/j.eti.2024.103772
Giovanna Pesante , Chiara Tesoriero , Emma Cadoria , Marco Andreolli , Silvia Lampis , Andrea Vettori , Nicola Frison

Unconventional protein sources are necessary to tackle the increasing demand for food. Microbial proteins (MP) are an alternative source of proteins for feed or food, suitable as feed for aquaculture. Substituting fishmeal with MP obtained from agricultural wastes could reduce the environmental burden of aquaculture and help with waste management. In this study, pure culture MP from the PHA-producer Thauera sp. Sel9 were obtained from agricultural residues (agro-zootechnical digestate and pasta industry leftovers). The produced MP was used in feeding tests with the model fish zebrafish (Danio rerio) to assess potential toxic effects and evaluate overall fish health. The obtained MP was rich in protein (59.5 % w/w over TS) and PHAs (15.0 %) and comprised all fish essential amino acids. The chemical scores and essential amino acid index confirmed the excellent quality of the MP. The feeding tests with 50 % feed substitution with MP resulted in survival rates (80–88 %) comparable to the control group (78 %), with only 100 % MP showing increased mortality. Thauera MP obtained from agricultural residues has the potential to become a partial fishmeal substitute in fish-farming.

要满足日益增长的食品需求,就必须有非常规的蛋白质来源。微生物蛋白质(MP)是饲料或食品蛋白质的替代来源,适合作为水产养殖的饲料。用从农业废弃物中获取的微生物蛋白替代鱼粉,可以减轻水产养殖的环境负担,并有助于废物管理。在这项研究中,从农业残留物(农业沼渣和面食工业残留物)中获得了来自 PHA 生产者 Sel9 藻类的纯培养 MP。生产的 MP 被用于模式鱼斑马鱼()的喂养试验,以评估潜在的毒性作用和鱼类的整体健康状况。获得的 MP 富含蛋白质(59.5% w/w 高于 TS)和 PHAs(15.0%),并含有所有鱼类必需氨基酸。化学评分和必需氨基酸指数证实了 MP 的优良品质。用 MP 代替 50 % 饲料进行喂养试验的结果是,存活率(80-88 %)与对照组(78 %)相当,只有 100 % 的 MP 会增加死亡率。从农业残留物中提取的 MP 有潜力成为养鱼业中的部分鱼粉替代品。
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引用次数: 0
Effects of straw and roots removal on soil Cd availability and Cd accumulation in rice at different growth stages 去除稻草和稻根对水稻不同生长阶段土壤镉供应量和镉积累的影响
IF 6.7 2区 环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-07-26 DOI: 10.1016/j.eti.2024.103768
Chang Li , Zi-Yu Wang , Hang Zhou , Yao-Lin Wen , Peng Zeng , Jiao-Feng Gu , Lu Hu , Hai-Wei Yuan , Bo-Han Liao

It is argument on whether straw removal present a safer alternative compared to straw return or not where in paddy fields contaminated with cadmium (Cd). The objective of this study was to evaluate the impacts of varying levels of straw and roots removal on Cd uptake and accumulation, as well as on the growth of rice, Cd availability of soil at different growth stages, and the safety and nutritional value of brown rice were subject to assessment as well. A field experiment was conducted wherein rice straw and roots were returned into the paddy field, serving as the control group (CK). The findings revealed that the removal of straw and roots resulted in a decline in the availability of Cd in soil and the accumulation and uptake of Cd by rice plant. At the maturation stage of rice, the soil available Cd content and brown rice Cd content was significantly reduced by 40.39% and24.79 % under the treatment where 100 % of rice straw and roots were removed. Moreover, there was a significant decline of 66.54 % and 76.35 % in dissolved organic carbon (DOC) and Cd concentrations respectively within soil pore water. This suggests that one crucial factor contributing to decreased Cd accumulation is the diminished complexation between DOC and Cd resulting from straw removal treatments. The removal of straw and roots had minimal impact on the nutritional components of brown rice, including essential amino acids. After the removal of straw and roots from the field, there was a reduction in hazard quotient (HQ) for rice consumers of varying genders and ages in the region by 17.71–24.95 %, leading to a decrease in local ecological risk level from medium to slight. Therefore, the implementation of strategies such as removing straw and roots could potentially lead to successful outcomes in reducing rice Cd uptake in paddy fields contaminated with this metallic element.

在受到镉(Cd)污染的稻田中,稻草清除是否比稻草还田更安全,目前还存在争议。本研究的目的是评估不同程度的稻草和稻根清除对镉吸收和积累的影响,以及对水稻生长、不同生长阶段土壤中镉的可用性、糙米的安全性和营养价值的影响。在田间试验中,稻草和稻根被送回稻田,作为对照组(CK)。研究结果表明,清除稻草和稻根会导致土壤中镉的供应量下降,以及水稻植株对镉的积累和吸收。在水稻成熟期,100%去除稻草和稻根的处理中,土壤可利用镉含量和糙米镉含量分别显著降低了 40.39% 和 24.79%。此外,土壤孔隙水中的溶解有机碳 (DOC) 和镉浓度也分别大幅下降了 66.54 % 和 76.35 %。这表明,导致镉积累减少的一个关键因素是去除秸秆后 DOC 和镉之间的复合物减少了。去除稻草和根对糙米的营养成分(包括必需氨基酸)影响很小。从田间清除稻草和根茎后,该地区不同性别和年龄的水稻消费者的危害商数(HQ)降低了 17.71-24.95%,导致当地的生态风险等级从中度降低到轻度。因此,在受镉金属元素污染的稻田中,实施清除稻草和稻根等策略有可能成功减少水稻对镉的吸收。
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引用次数: 0
Phosphate chelation over calcium impacts yeast growth and lipid production from short-chain fatty acids-rich media 磷酸螯合作用对富含短链脂肪酸培养基中酵母生长和脂质生产的影响
IF 6.7 2区 环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-07-26 DOI: 10.1016/j.eti.2024.103767
Sergio Morales-Palomo , Elia Tomás-Pejó , Cristina González-Fernández

Some oleaginous yeasts have the ability to produce microbial oils from alternative carbon sources, such as short-chain fatty acids (SCFAs). Nevertheless, there is still a lack of information about the possible effects that media nutrients have on yeast metabolisms when using SCFAs. For instance, inorganic phosphate (PO43-) has been reported to promote yeast growth in literature but its chelating effect over other elements such as calcium (Ca2+) is often not considered in fermentation processes while limitation of nitrogen is probably the most studied. Attending at the need to better understand the role of PO43-, this work assessed the lipid production capacity of Yarrowia lipolytica ACA DC 50109, both in synthetic and real SCFAs-rich media, at different SCFAs concentrations and PO43-:Ca2+ ratios. Reducing PO43-:Ca2+ ratio was identified to be an important factor to improve yeast growth, reaching the highest lipid content (52.7 ± 0.9 % w/w) and lipid yield (0.31 ± 0.01 w/w) in media without PO43-. These results demonstrated the importance of Ca2+ availability in the medium and nutrients interactions in yeast growth that are often underestimated.

一些含油酵母能够利用短链脂肪酸(SCFA)等替代碳源生产微生物油。然而,目前仍缺乏有关使用 SCFAs 时培养基养分对酵母新陈代谢可能产生的影响的信息。例如,有文献报道无机磷酸盐(PO43-)可促进酵母生长,但在发酵过程中往往没有考虑其对其他元素(如钙(Ca2+))的螯合作用,而对氮的限制可能是研究最多的。为了更好地了解 PO43- 的作用,本研究评估了脂肪溶解亚罗酵母(Yarrowia lipolytica ACA DC 50109)在富含 SCFAs 的合成培养基和真实培养基中,不同 SCFAs 浓度和 PO43-:Ca2+ 比例下的脂质生产能力。在不含 PO43- 的培养基中,脂质含量(52.7 ± 0.9 % w/w)和脂质产量(0.31 ± 0.01 w/w)最高。这些结果表明,培养基中 Ca2+ 的可用性和养分相互作用在酵母生长中的重要性往往被低估。
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引用次数: 0
Development of highly porous seaweed impregnated facemask substrates for protecting people from microbial hazards 开发高多孔海藻浸渍面罩基材,保护人们免受微生物危害
IF 6.7 2区 环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-07-22 DOI: 10.1016/j.eti.2024.103764
P. Nisha , Shahma R.M , Sandhanasamy Devanesan , Tanveer Ahmad Mir , Raja Chinnappan , Mohamad S. AlSalhi , Tariq Alzahim

The present study reports the development of a biodegradable mask by combined usage of Lessonia nigrescens(a species of brown algae), Rhizoclonium riparium (a cryptogenic macroalga), and Aloe vera (a succulent plant). The proposed face mask is comprised of three stacked layers: an inner hydrophilic layer containing macroalgae, an intermediate layer coated with microalgae extracts, and an outer hydrophobic layer reinforced with Aloe vera and hexadecyltrimethoxysilane (HDTMS). Experimental investigations confirmed that using seaweed derivatives and Aloe vera plant-based bioactive compounds as coating agents ensures chemical and thermal resilience and can be reused after washing. The ensuring topographic and chemical configurations were characterized through scanning electron microscopy and Fourier transform infrared spectroscopy. The surface wettability of the material was tested by the water contact angle. The resultant facemask products exhibit remarkable water repellency, stain resistance, antimicrobial and antifungal performance against pathogens. Our results suggest that the proposed eco-friendly technique for developing facemask surfaces with inherent antimicrobial and antifungal features might be attractive to personal protective equipment manufacturers and environmental material engineering investigators. The proposed mask offers improved water and aerosol repellency, increased air permeability, and enhanced skin-friendliness compared to masks currently available in the market. Additionally, this innovative mask is suitable for use during mass infections and is designed to be worn for extended periods without causing skin irritation or breathing problems. However, if these features are indeed present in the newly manufactured masks, they could offer significant advantages in terms of protection and comfort in a variety of situations, including outbreaks.

本研究报告介绍了通过联合使用 Lessonia nigrescens(一种褐藻)、Rhizoclonium riparium(一种隐生大型藻类)和芦荟(一种肉质植物)开发出的可生物降解面膜。拟议中的面膜由三层叠加层组成:内层亲水层含有大型海藻,中间层涂有微藻提取物,外层疏水层由芦荟和十六烷基三甲氧基硅烷(HDTMS)加固。实验研究证实,使用海藻衍生物和芦荟植物基生物活性化合物作为涂层剂可确保化学和热弹性,并可在清洗后重复使用。通过扫描电子显微镜和傅立叶变换红外光谱,对确保的形貌和化学结构进行了表征。通过水接触角测试了材料的表面润湿性。结果表明,面罩产品具有显著的防水、防污、抗菌和抗真菌性能。我们的研究结果表明,所提出的开发具有固有抗菌和抗真菌功能的面罩表面的环保技术可能对个人防护设备制造商和环境材料工程研究人员具有吸引力。与目前市场上销售的口罩相比,拟议的口罩具有更好的防水和防气溶胶性能、更高的透气性和更强的亲肤性。此外,这种创新型口罩还适合在大规模感染时使用,并可长时间佩戴而不会造成皮肤过敏或呼吸困难。不过,如果新生产的口罩确实具备这些功能,那么在包括疾病爆发在内的各种情况下,它们在保护和舒适性方面都将具有显著优势。
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引用次数: 0
The enrichment of antibiotic resistance genes in swine manure compost was related to the bulking agent types 猪粪堆肥中抗生素抗性基因的富集与膨松剂类型有关
IF 6.7 2区 环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-07-22 DOI: 10.1016/j.eti.2024.103765
Jiani Wang , Xia Gao , Guoying Wang , Ying Liu , Jiali Chang , Tao Jiang , Guoxue Li , Ruonan Ma , Yan Yang , Jing Yuan

Composting is a major method to produce organic fertilizers, and the variation in antibiotic resistance genes (ARGs) during composting is crucial for the safe utilization of mature compost. The effects of different bulking agents (cellulose-rich cornstalk and lignin-rich garden waste) on ARGs variations during swine manure composting were investigated. The results showed that composting thermophilic could effectively reduce ARGs (58–61 %), whereas ARGs rebounded and were enriched with decreasing temperature during the maturation stage. Compared to their initial abundance, ARGs were enriched 6.97 times (cornstalk) and 22.27 times (garden waste) during the maturation period. The ARGs enrichment mechanism in swine manure composting differed for cornstalk and garden waste amendments. The cornstalk was used as the bulking agent, the selective pressure of continuous high temperature resulted in the proliferation of spore-forming bacteria (Bacillus, Sporosarcina and Psychrobacillus), which are potential host bacteria of ARGs and cause ARGs enrichment through vertical gene proliferation. In the garden waste treatment, the enriched ARGs in the final compost were related to horizontal gene transfer mediated by mobile genetic elements (intl1), with the primary potential host bacteria being Bacillus, Saccharomonospora, and Caldicoprobacter. The types of enriched ARGs were consistent across different bulking agents composting process, and the risk genes enriched in the final mature compost included ermB, ermF, sul1, sul2, tetO, and tetX.

堆肥是生产有机肥的一种主要方法,而堆肥过程中抗生素抗性基因(ARGs)的变化对于安全利用成熟堆肥至关重要。研究人员调查了不同膨松剂(富含纤维素的玉米秸秆和富含木质素的园林垃圾)对猪粪堆肥过程中抗生素抗性基因变化的影响。结果表明,嗜热堆肥可有效减少 ARGs(58-61%),而在成熟阶段,随着温度的降低,ARGs 会反弹并富集。与初始丰度相比,成熟期 ARGs 富集了 6.97 倍(玉米秸秆)和 22.27 倍(花园垃圾)。在猪粪堆肥过程中,玉米秸秆和园林废弃物添加剂的 ARGs 富集机制不同。玉米秸秆作为膨松剂,持续高温的选择性压力导致芽孢形成菌(芽孢杆菌、孢子丝菌和精神杆菌)增殖,而芽孢杆菌、孢子丝菌和精神杆菌是 ARGs 的潜在宿主菌,通过垂直基因增殖导致 ARGs 富集。在花园垃圾处理中,最终堆肥中富集的 ARGs 与移动遗传因子(intl1)介导的水平基因转移有关,主要潜在宿主菌是芽孢杆菌、酵母菌和钙化杆菌。不同膨松剂堆肥过程中富集的 ARGs 类型一致,最终成熟堆肥中富集的风险基因包括 ermB、ermF、sul1、sul2、tetO 和 tetX。
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引用次数: 0
Mitigation of asphalt volatile organic compounds emissions and health hazards using a TiO2-doped biochar composite: Microscopic and physiological insights 使用掺杂 TiO2 的生物炭复合材料缓解沥青挥发性有机化合物排放和健康危害:微观和生理学见解
IF 6.7 2区 环境科学与生态学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-07-22 DOI: 10.1016/j.eti.2024.103763
Lei Ge , Yongsheng Yao , Linghong Xu , Zizun Zhou , Jue Li , Xinqiang Zhang , Chaochao Liu , Huiqing Lv

This study developed a novel TiO2-biochar composite as a modifier for functional asphalt coatings, achieving dual objectives of reducing VOCs emissions and mitigating cytotoxicity. The composite enhanced the softening point of the asphalt by approximately 6℃, reduced penetration and increased viscosity, thereby improving thermal stability and deformation resistance. Headspace-gas chromatography/mass spectrometry analysis confirmed an over 80 % reduction in VOC emissions, significantly reducing the release of harmful compounds like alkanes, cycloalkanes, and aromatic hydrocarbons, with the TiO2-biochar modified and UV-exposed (TUBC) demonstrating the most pronounced effect. In vitro assays with human bronchial epithelial (BEAS-2B) cells showed the composite's cytotoxicity mitigation, with TUBC maintaining higher cell viability. The composite reduced ROS levels and the expression of cytotoxicity-associated biomarkers, suggesting a decrease in oxidative stress and inflammation. The high adsorption capacity of the composite and its photocatalytic degradation under UV light were identified as the key mechanisms for VOCs reduction. These findings collectively establish the TiO2-biochar composite as a promising solution for asphalt for greener and safer asphalt applications, with potential for global environmental and health benefits. Future work will focus on further optimization and field validation to facilitate the adoption of this technology in infrastructure development worldwide.

本研究开发了一种新型二氧化钛-生物炭复合材料,作为功能性沥青涂料的改性剂,实现了减少挥发性有机化合物排放和减轻细胞毒性的双重目标。该复合材料将沥青的软化点提高了约 6℃,降低了渗透性并增加了粘度,从而提高了热稳定性和抗变形性。顶空气相色谱/质谱分析证实,挥发性有机化合物的排放量减少了 80% 以上,大大减少了烷烃、环烷烃和芳香烃等有害化合物的释放,其中二氧化钛-生物炭改性和紫外线照射(TUBC)的效果最为明显。用人类支气管上皮细胞(BEAS-2B)进行的体外试验表明,复合材料的细胞毒性有所减轻,其中 TUBC 的细胞存活率更高。复合材料降低了 ROS 水平和细胞毒性相关生物标志物的表达,表明氧化应激和炎症有所减轻。复合材料的高吸附能力及其在紫外线下的光催化降解被认为是减少挥发性有机化合物的关键机制。这些研究结果共同确定了二氧化钛-生物炭复合材料是一种很有前景的沥青解决方案,可用于更环保、更安全的沥青应用,并有可能为全球环境和健康带来益处。未来的工作重点是进一步优化和现场验证,以促进该技术在全球基础设施建设中的应用。
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