Pub Date : 2024-12-01DOI: 10.1016/j.eng.2024.10.002
Donglian Gu , Qingrui Yue , Li Li , Chujin Sun , Xinzheng Lu
Vision-based digital shadowing is a highly efficient way to monitor the health of buildings in use. However, previous studies on digital shadowing have been limited to laboratory experiments. This paper proposes a novel computer-vision-based digital shadow workflow and presents its successful application in a real engineering case. In this case, a 345.8-m supertall building experienced unexpected shaking under normal meteorological conditions. This study established a digital shadow of the building using three-dimensional displacement measurements based on super-resolution monocular vision, revealing the hidden structural dynamics and inherent mechanical reasons for the abnormal shaking. The proposed digital shadowing workflow is a feasible roadmap for developing vision-based digital shadows of real-world structures using low-cost cameras. The abnormal vibration event in the supertall building considered in this study is the first of its type worldwide. The results of this study offer practical strategies and invaluable insights into the prevention and mitigation of this type of global risk, thereby contributing to the lifespan extension of buildings in use worldwide. Furthermore, with the increasing number of general sensing devices, such as surveillance cameras in cities, the proposed method may unleash the immense potential of general sensing devices in achieving the leap from structural health monitoring to city health monitoring.
{"title":"Vision-Based Digital Shadowing to Reveal Hidden Structural Dynamics of a Real Supertall Building","authors":"Donglian Gu , Qingrui Yue , Li Li , Chujin Sun , Xinzheng Lu","doi":"10.1016/j.eng.2024.10.002","DOIUrl":"10.1016/j.eng.2024.10.002","url":null,"abstract":"<div><div>Vision-based digital shadowing is a highly efficient way to monitor the health of buildings in use. However, previous studies on digital shadowing have been limited to laboratory experiments. This paper proposes a novel computer-vision-based digital shadow workflow and presents its successful application in a real engineering case. In this case, a 345.8-m supertall building experienced unexpected shaking under normal meteorological conditions. This study established a digital shadow of the building using three-dimensional displacement measurements based on super-resolution monocular vision, revealing the hidden structural dynamics and inherent mechanical reasons for the abnormal shaking. The proposed digital shadowing workflow is a feasible roadmap for developing vision-based digital shadows of real-world structures using low-cost cameras. The abnormal vibration event in the supertall building considered in this study is the first of its type worldwide. The results of this study offer practical strategies and invaluable insights into the prevention and mitigation of this type of global risk, thereby contributing to the lifespan extension of buildings in use worldwide. Furthermore, with the increasing number of general sensing devices, such as surveillance cameras in cities, the proposed method may unleash the immense potential of general sensing devices in achieving the leap from structural health monitoring to city health monitoring.</div></div>","PeriodicalId":11783,"journal":{"name":"Engineering","volume":"43 ","pages":"Pages 146-158"},"PeriodicalIF":10.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143326770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.eng.2024.06.004
Guangwei Wang , Xueming Liu , Ying Xiao , Ye Yuan , Linqiang Pan , Xiaohong Guan , Jianxi Gao , Hai-Tao Zhang
Ecosystems are undergoing unprecedented persistent deterioration due to unsustainable anthropogenic human activities, such as overfishing and deforestation, and the effects of such damage on ecological stability are uncertain. Despite recent advances in experimental and theoretical studies on regime shifts and tipping points, theoretical tools for understanding the extinction chain, which is the sequence of species extinctions resulting from overexploitation, are still lacking, especially for large-scale nonlinear networked systems. In this study, we developed a mathematical tool to predict regime shifts and extinction chains in ecosystems under multiple exploitation situations and verified it in 26 real-world mutualistic networks of various sizes and densities. We discovered five phases during the exploitation process: safe, partial extinction, bistable, tristable, and collapse, which enabled the optimal design of restoration strategies for degraded or collapsed systems. We validated our approach using a 20-year dataset from an eelgrass restoration project. Counterintuitively, we also found a specific region in the diagram spanning exploitation rates and competition intensities, where exploiting more species helps increase biodiversity. Our computational tool provides insights into harvesting, fishing, exploitation, or deforestation plans while conserving or restoring the biodiversity of mutualistic ecosystems.
{"title":"Extinction Chains Reveal Intermediate Phases Between the Safety and Collapse in Mutualistic Ecosystems","authors":"Guangwei Wang , Xueming Liu , Ying Xiao , Ye Yuan , Linqiang Pan , Xiaohong Guan , Jianxi Gao , Hai-Tao Zhang","doi":"10.1016/j.eng.2024.06.004","DOIUrl":"10.1016/j.eng.2024.06.004","url":null,"abstract":"<div><div>Ecosystems are undergoing unprecedented persistent deterioration due to unsustainable anthropogenic human activities, such as overfishing and deforestation, and the effects of such damage on ecological stability are uncertain. Despite recent advances in experimental and theoretical studies on regime shifts and tipping points, theoretical tools for understanding the extinction chain, which is the sequence of species extinctions resulting from overexploitation, are still lacking, especially for large-scale nonlinear networked systems. In this study, we developed a mathematical tool to predict regime shifts and extinction chains in ecosystems under multiple exploitation situations and verified it in 26 real-world mutualistic networks of various sizes and densities. We discovered five phases during the exploitation process: safe, partial extinction, bistable, tristable, and collapse, which enabled the optimal design of restoration strategies for degraded or collapsed systems. We validated our approach using a 20-year dataset from an eelgrass restoration project. Counterintuitively, we also found a specific region in the diagram spanning exploitation rates and competition intensities, where exploiting more species helps increase biodiversity. Our computational tool provides insights into harvesting, fishing, exploitation, or deforestation plans while conserving or restoring the biodiversity of mutualistic ecosystems.</div></div>","PeriodicalId":11783,"journal":{"name":"Engineering","volume":"43 ","pages":"Pages 89-98"},"PeriodicalIF":10.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141711462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.eng.2024.08.025
Xue Xiong , Qingji Huo , Changpeng Cui , Uma K. Aryal , BonHeon Ku , Chin-Suk Hong , HeeChang Lim , Jing Liu , Andy Chen , William R. Thompson , Bai-Yan Li , Xue-Lian Li , Hiroki Yokota
Bone frequently serves as a metastatic site for breast and prostate cancers. Given the potential of low-intensity vibration (LIV) to increase bone health and reduce cancer risk, this study investigated the impact of LIV on cancer cells, as well as noncancer cells such as lymphocytes and peripheral blood mononuclear cells (PBMCs). The results revealed that LIV exposure not only suppressed cancer cell migration but also triggered the generation of induced tumor-suppressing (iTS) cells. Conditioned medium (CM) derived from LIV-treated PBMCs shrank freshly isolated breast and prostate cancer tissues, and when CM was combined with a chemotherapeutic agent, additional antitumor effects were observed. Notably, iTS cell-derived CM hindered the maturation of the receptor activator of nuclear factor-kappa B ligand (RANKL)-stimulated bone-resorbing osteoclasts while promoting the differentiation of bone-forming osteoblasts. Intriguingly, the anticancer effects induced by LIV were replicated by simply shaking a cell-containing tube with a regular tube shaker. Using mass spectrometry-based proteomics, this study revealed enrichment of tumor-suppressing proteins, including enolase 1, moesin (MSN), and aldolase A (ALDOA), which are commonly found in oncogene-activated iTS cells, in LIV-induced CM. Sad1 and UNC-84 domain containing 1 (SUN1), a core component of the linker of the nucleoskeleton and cytoskeleton (LINC) complex, exhibited heightened expression, notably enhancing the response of lymphocytes to LIV. An ex vivo bone cancer model further demonstrated the potent anticancer effects of lymphocyte-derived CM. In conclusion, this study underscores the pivotal role of LIV in preventing bone loss in the tumor microenvironment.
{"title":"Therapeutic Insights into Low-intensity Vibration for Generating Induced Tumor-Suppressive Cells and Modulating the Bone Microenvironment","authors":"Xue Xiong , Qingji Huo , Changpeng Cui , Uma K. Aryal , BonHeon Ku , Chin-Suk Hong , HeeChang Lim , Jing Liu , Andy Chen , William R. Thompson , Bai-Yan Li , Xue-Lian Li , Hiroki Yokota","doi":"10.1016/j.eng.2024.08.025","DOIUrl":"10.1016/j.eng.2024.08.025","url":null,"abstract":"<div><div>Bone frequently serves as a metastatic site for breast and prostate cancers. Given the potential of low-intensity vibration (LIV) to increase bone health and reduce cancer risk, this study investigated the impact of LIV on cancer cells, as well as noncancer cells such as lymphocytes and peripheral blood mononuclear cells (PBMCs). The results revealed that LIV exposure not only suppressed cancer cell migration but also triggered the generation of induced tumor-suppressing (iTS) cells. Conditioned medium (CM) derived from LIV-treated PBMCs shrank freshly isolated breast and prostate cancer tissues, and when CM was combined with a chemotherapeutic agent, additional antitumor effects were observed. Notably, iTS cell-derived CM hindered the maturation of the receptor activator of nuclear factor-kappa B ligand (RANKL)-stimulated bone-resorbing osteoclasts while promoting the differentiation of bone-forming osteoblasts. Intriguingly, the anticancer effects induced by LIV were replicated by simply shaking a cell-containing tube with a regular tube shaker. Using mass spectrometry-based proteomics, this study revealed enrichment of tumor-suppressing proteins, including enolase 1, moesin (MSN), and aldolase A (ALDOA), which are commonly found in oncogene-activated iTS cells, in LIV-induced CM. Sad1 and UNC-84 domain containing 1 (SUN1), a core component of the linker of the nucleoskeleton and cytoskeleton (LINC) complex, exhibited heightened expression, notably enhancing the response of lymphocytes to LIV. An <em>ex vivo</em> bone cancer model further demonstrated the potent anticancer effects of lymphocyte-derived CM. In conclusion, this study underscores the pivotal role of LIV in preventing bone loss in the tumor microenvironment.</div></div>","PeriodicalId":11783,"journal":{"name":"Engineering","volume":"43 ","pages":"Pages 201-215"},"PeriodicalIF":10.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143326744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.eng.2024.03.018
Hong Liu , Zhaoming Chen , Zhiwei Wang , Ming Xu , Yutao Wang , Jinju Geng , Fengjun Yin
Water quality system informatics (WQSI) is an emerging field that employs cybernetics to collect and digitize data associated with water quality. It involves monitoring the physical, chemical, and biological processes that affect water quality and the ecological impacts and interconnections within water quality systems. WQSI integrates theories and methods from water quality engineering, information engineering, and system control theory, enabling the intelligent management and control of water quality. This integration revolutionizes the understanding and management of water quality systems with greater precision and higher resolution. WQSI is a new stage of development in environmental engineering that is driven by the digital age. This work explores the fundamental concepts, research topics, and methods of WQSI and its features and potential to promote disciplinary development. The innovation and development of WQSI are crucial for driving the digital and intelligent transformation of national industry patterns in China, positioning China at the forefront of environmental engineering and ecological environment research on a global scale.
{"title":"Water Quality System Informatics: An Emerging Inter-Discipline of Environmental Engineering","authors":"Hong Liu , Zhaoming Chen , Zhiwei Wang , Ming Xu , Yutao Wang , Jinju Geng , Fengjun Yin","doi":"10.1016/j.eng.2024.03.018","DOIUrl":"10.1016/j.eng.2024.03.018","url":null,"abstract":"<div><div>Water quality system informatics (WQSI) is an emerging field that employs cybernetics to collect and digitize data associated with water quality. It involves monitoring the physical, chemical, and biological processes that affect water quality and the ecological impacts and interconnections within water quality systems. WQSI integrates theories and methods from water quality engineering, information engineering, and system control theory, enabling the intelligent management and control of water quality. This integration revolutionizes the understanding and management of water quality systems with greater precision and higher resolution. WQSI is a new stage of development in environmental engineering that is driven by the digital age. This work explores the fundamental concepts, research topics, and methods of WQSI and its features and potential to promote disciplinary development. The innovation and development of WQSI are crucial for driving the digital and intelligent transformation of national industry patterns in China, positioning China at the forefront of environmental engineering and ecological environment research on a global scale.</div></div>","PeriodicalId":11783,"journal":{"name":"Engineering","volume":"43 ","pages":"Pages 115-124"},"PeriodicalIF":10.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141142665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.eng.2024.09.021
Xuan Chen , Wei Luo , Qun Gao , Congrong Chen , Lichan Li , Dongbo Liu , Shaoyun Wang
A sustainable solution to the dramatic spread of antibiotic resistance threatening public health security is the development of antibiotic-free antimicrobial substances. Inspired by natural host defense mechanisms involving amino-terminal copper–nickel binding motif (ATCUN) antimicrobial peptides (AMPs), we have designed and prepared an artificial complex (Cu@G-AMPs) incorporating single-atom Cu catalysts for antibacterial therapy. The substrate of the complex, formed from guanine doped with abundant heteroatoms, anchored single Cu atoms with a coordination number of 2 and an average bond length of 1.91 Å. Interestingly, Cu@G-AMPs, exhibiting Fenton-like catalytic activity, caused the inactivation of methicillin-resistant Staphylococcus aureus (MRSA) by generating and delivering reactive oxygen species (ROS) cargo. Mechanistically, the intrinsic stress response system of MRSA underwent an irreversible collapse when Cu@G-AMPs initiated its offensive program associated with non-specific targets. Furthermore, Cu@G-AMPs, which inherited the immunomodulatory properties of AMPs, sequentially carried out the functions of pulling edge closure, stabilizing granulation tissue, promoting collagen fiber proliferation, alleviating inflammation, and promoting neovascularization in wound areas infected by MRSA. Our results show that Cu@G-AMPs will provide a new perspective on untangling the complex regulatory networks that resistant bacteria have cultivated to deactivate commercial antibiotics.
{"title":"Incorporating Single-Copper Sites and Host Defense Peptides into a Nanoreactor for Antibacterial Therapy by Bioinspired Design","authors":"Xuan Chen , Wei Luo , Qun Gao , Congrong Chen , Lichan Li , Dongbo Liu , Shaoyun Wang","doi":"10.1016/j.eng.2024.09.021","DOIUrl":"10.1016/j.eng.2024.09.021","url":null,"abstract":"<div><div>A sustainable solution to the dramatic spread of antibiotic resistance threatening public health security is the development of antibiotic-free antimicrobial substances. Inspired by natural host defense mechanisms involving amino-terminal copper–nickel binding motif (ATCUN) antimicrobial peptides (AMPs), we have designed and prepared an artificial complex (Cu@G-AMPs) incorporating single-atom Cu catalysts for antibacterial therapy. The substrate of the complex, formed from guanine doped with abundant heteroatoms, anchored single Cu atoms with a coordination number of 2 and an average bond length of 1.91 Å. Interestingly, Cu@G-AMPs, exhibiting Fenton-like catalytic activity, caused the inactivation of methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) by generating and delivering reactive oxygen species (ROS) cargo. Mechanistically, the intrinsic stress response system of MRSA underwent an irreversible collapse when Cu@G-AMPs initiated its offensive program associated with non-specific targets. Furthermore, Cu@G-AMPs, which inherited the immunomodulatory properties of AMPs, sequentially carried out the functions of pulling edge closure, stabilizing granulation tissue, promoting collagen fiber proliferation, alleviating inflammation, and promoting neovascularization in wound areas infected by MRSA. Our results show that Cu@G-AMPs will provide a new perspective on untangling the complex regulatory networks that resistant bacteria have cultivated to deactivate commercial antibiotics.</div></div>","PeriodicalId":11783,"journal":{"name":"Engineering","volume":"43 ","pages":"Pages 216-227"},"PeriodicalIF":10.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143326771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pesticide ecological safety continues to be a hot issue. The inherent biosafety and physiological functions of vanillin, a widely used natural flavor in food additives, have unlocked numerous applications in the medical field, leading to a plethora of pharmaceutically active derivatives and commercial drugs. Despite its extensive use in pharmaceutical discovery and the food industry, vanillin’s potential in the domain of green pesticide development has only recently come to light. Significantly, its advantages of safety and low price make vanillin ideal for green pesticide research and development (R&D). In this context, this review illuminates the research on vanillin’s transformation into a suite of innovative agrochemicals. By delving into the design, synthesis, action mechanisms, and bio-safety of these vanillin-derived compounds, we uncover novel pathways for sustainable agriculture. Further possible directions for the exploration of this substance are also outlined. We believe that this story about vanillin will serve as a source of inspiration for those seeking to derive innovative ideas from natural substances, particularly in the realm of green pesticide R&D.
{"title":"Pesticide Engineering from Natural Vanillin: Recent Advances and a Perspective","authors":"Mingshu Lou, Sha Li, Fangru Jin, Tangbing Yang, Runjiang Song, Baoan Song","doi":"10.1016/j.eng.2024.06.015","DOIUrl":"10.1016/j.eng.2024.06.015","url":null,"abstract":"<div><div>Pesticide ecological safety continues to be a hot issue. The inherent biosafety and physiological functions of vanillin, a widely used natural flavor in food additives, have unlocked numerous applications in the medical field, leading to a plethora of pharmaceutically active derivatives and commercial drugs. Despite its extensive use in pharmaceutical discovery and the food industry, vanillin’s potential in the domain of green pesticide development has only recently come to light. Significantly, its advantages of safety and low price make vanillin ideal for green pesticide research and development (R&D). In this context, this review illuminates the research on vanillin’s transformation into a suite of innovative agrochemicals. By delving into the design, synthesis, action mechanisms, and bio-safety of these vanillin-derived compounds, we uncover novel pathways for sustainable agriculture. Further possible directions for the exploration of this substance are also outlined. We believe that this story about vanillin will serve as a source of inspiration for those seeking to derive innovative ideas from natural substances, particularly in the realm of green pesticide R&D.</div></div>","PeriodicalId":11783,"journal":{"name":"Engineering","volume":"43 ","pages":"Pages 241-257"},"PeriodicalIF":10.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141777019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.eng.2024.11.010
Comprehensive Group of the Global Engineering Front Research Project
{"title":"2024 Global Engineering Fronts","authors":"Comprehensive Group of the Global Engineering Front Research Project","doi":"10.1016/j.eng.2024.11.010","DOIUrl":"10.1016/j.eng.2024.11.010","url":null,"abstract":"","PeriodicalId":11783,"journal":{"name":"Engineering","volume":"43 ","pages":"Pages 4-7"},"PeriodicalIF":10.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143358300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.eng.2024.06.011
Junyao Xiong , Usama Ashraf , Jing Ye , Shengbo Cao
Extracellular vesicles (EVs) are secreted by cells and widely exist in body fluids, serving as an essential vehicle of intercellular communication. In recent years, EVs have gained significant attention owing to their ability to carry nucleic acids, lipids, and proteins of host and pathogen origins. A distinct composition of EVs during pathogenic infection contributes to mediating pathogenic infection, transmission, and immunity to target cells. Therefore, studying the role of EVs in pathogenic infection is crucial for understanding pathogen–host interactions and developing new anti-pathogenic therapies. This review offers an overview of current knowledge of EVs in the context of infection, transmission, and immunity to pathogens. Harnessing EVs’ biology during pathogenic infection may lay a foundation for the mitigation of pathogenic infection and associated disease outcomes.
{"title":"Extracellular Vesicles in Pathogenic Infection, Transmission, and Immunity","authors":"Junyao Xiong , Usama Ashraf , Jing Ye , Shengbo Cao","doi":"10.1016/j.eng.2024.06.011","DOIUrl":"10.1016/j.eng.2024.06.011","url":null,"abstract":"<div><div>Extracellular vesicles (EVs) are secreted by cells and widely exist in body fluids, serving as an essential vehicle of intercellular communication. In recent years, EVs have gained significant attention owing to their ability to carry nucleic acids, lipids, and proteins of host and pathogen origins. A distinct composition of EVs during pathogenic infection contributes to mediating pathogenic infection, transmission, and immunity to target cells. Therefore, studying the role of EVs in pathogenic infection is crucial for understanding pathogen–host interactions and developing new anti-pathogenic therapies. This review offers an overview of current knowledge of EVs in the context of infection, transmission, and immunity to pathogens. Harnessing EVs’ biology during pathogenic infection may lay a foundation for the mitigation of pathogenic infection and associated disease outcomes.</div></div>","PeriodicalId":11783,"journal":{"name":"Engineering","volume":"43 ","pages":"Pages 228-240"},"PeriodicalIF":10.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141696441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.eng.2024.07.007
Chuanxi Tian , Hang Liu , Qian Wang , Jinyue Zhao , Chensi Yao , Yanfeng Yao , Xu Zhang , Qinhai Ma , Weihao Wang , Yanyan Zhou , Mengxiao Wang , Xiaomeng Shi , Xiangyan Li , Shan Wang , Yingying Yang , Xiaowen Gou , Lijuan Zhou , Jingyi Zhao , Li Wan , Jiarui Li , Xiaolin Tong
Sanhan Huashi formula (SHHS), a traditional Chinese medicine (TCM), has shown significant therapeutic effects on coronavirus disease 2019 (COVID-19) in clinical settings. However, its specific mechanism and components still require further clarification. In vitro experiments with Vero-E6 cells infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demonstrated that SHHS effectively inhibited viral invasion and proliferation. Complementary in vivo experiments using K18-human angiotensin converting enzyme 2 (hACE2) mice exposed to virus-like particles (VLPs) further confirmed that SHHS impeded SARS-CoV-2 entry. Although SHHS did not demonstrate direct antiviral effects in K18-hACE2 mice challenged with SARS-CoV-2, it significantly alleviated pathological damage and decreased the expression of chemokines such as C–C motif ligand (CCL)-2, CCL-3, C–X–C motif ligand (CXCL)-1, CXCL-6, CXCL-9, CXCL-10, and CXCL-11 in the lungs, suggesting that SHHS exerts immunomodulatory and anti-inflammatory effects via the CCL-2–CXCL axis. Additional research using a lipopolysaccharide (LPS)-induced acute lung injury (ALI) and RAW264.7 cell model validated the ability of SHHS to reduce the levels of inflammatory biomarkers, including interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α). Using advanced analytical techniques such as ultrahigh-performance liquid chromatography coupled with linear trap quadrupole Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap-MS) and surface plasmon resonance (SPR), nodakenin was identified as a potent antiviral component of SHHS that targets the 3C-like protease (3CLpro), a finding supported by the hydrogen–deuterium exchange mass spectrometry (HDX-MS) and molecular docking analyses. Furthermore, nodakenin demonstrated a significant antiviral effect, reducing the viral load by more than 66%. This investigation reveals that SHHS can combat COVID-19 by inhibiting viral invasion and promoting anti-inflammatory effects.
{"title":"Sanhan Huashi Formula and Its Bioactive Compounds Exert Antiviral and Anti-Inflammatory Effects on COVID-19","authors":"Chuanxi Tian , Hang Liu , Qian Wang , Jinyue Zhao , Chensi Yao , Yanfeng Yao , Xu Zhang , Qinhai Ma , Weihao Wang , Yanyan Zhou , Mengxiao Wang , Xiaomeng Shi , Xiangyan Li , Shan Wang , Yingying Yang , Xiaowen Gou , Lijuan Zhou , Jingyi Zhao , Li Wan , Jiarui Li , Xiaolin Tong","doi":"10.1016/j.eng.2024.07.007","DOIUrl":"10.1016/j.eng.2024.07.007","url":null,"abstract":"<div><div>Sanhan Huashi formula (SHHS), a traditional Chinese medicine (TCM), has shown significant therapeutic effects on coronavirus disease 2019 (COVID-19) in clinical settings. However, its specific mechanism and components still require further clarification. <em>In vitro</em> experiments with Vero-E6 cells infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demonstrated that SHHS effectively inhibited viral invasion and proliferation. Complementary <em>in vivo</em> experiments using K18-human angiotensin converting enzyme 2 (hACE2) mice exposed to virus-like particles (VLPs) further confirmed that SHHS impeded SARS-CoV-2 entry. Although SHHS did not demonstrate direct antiviral effects in K18-hACE2 mice challenged with SARS-CoV-2, it significantly alleviated pathological damage and decreased the expression of chemokines such as C–C motif ligand (CCL)-2, CCL-3, C–X–C motif ligand (CXCL)-1, CXCL-6, CXCL-9, CXCL-10, and CXCL-11 in the lungs, suggesting that SHHS exerts immunomodulatory and anti-inflammatory effects via the CCL-2–CXCL axis. Additional research using a lipopolysaccharide (LPS)-induced acute lung injury (ALI) and RAW264.7 cell model validated the ability of SHHS to reduce the levels of inflammatory biomarkers, including interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α). Using advanced analytical techniques such as ultrahigh-performance liquid chromatography coupled with linear trap quadrupole Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap-MS) and surface plasmon resonance (SPR), nodakenin was identified as a potent antiviral component of SHHS that targets the 3C-like protease (3CL<sup>pro</sup>), a finding supported by the hydrogen–deuterium exchange mass spectrometry (HDX-MS) and molecular docking analyses. Furthermore, nodakenin demonstrated a significant antiviral effect, reducing the viral load by more than 66%. This investigation reveals that SHHS can combat COVID-19 by inhibiting viral invasion and promoting anti-inflammatory effects.</div></div>","PeriodicalId":11783,"journal":{"name":"Engineering","volume":"43 ","pages":"Pages 159-172"},"PeriodicalIF":10.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141776935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号