European Journal of Soil Biology最新文献_第4页

Microbial mechanism of soil organic carbon content under waterlogging and water table fluctuation in drained peat soils 涝渍和地下水位波动对排干泥炭土土壤有机碳含量的微生物机制

IF 3.3 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2025-09-13 DOI: 10.1016/j.ejsobi.2025.103773

Xiaohan Li , Yang Wang , Jiye Cai , Guangyuan Xu , Hongkai Li , Yanmin Dong , Shasha Liu , Ziping Liu , Shengzhong Wang , Zhiwei Xu

Peatlands serve as crucial reservoirs within the global carbon cycle; however, widespread degradation has led to significant reductions in soil organic carbon (SOC) contents. The effects of rewetting on the active SOC and the underlying microbial mechanisms in degraded peatlands are still not fully elucidated. This study examined changes in bulk and active SOC contents, as well as microbial community composition and enzyme activities via controlled water table (WT) manipulation in intact peat columns sourced from drained sites in the Changbai Mountains. The results demonstrated that variations in WT significantly influenced microbial biomass and enzymatic activities within the surface soil layer. Waterlogging resulted in decreased total bacterial and actinomycete biomass, accompanied by a marked increase in fungal biomass, especially under WT fluctuation. Soil oxidase and hydrolase activities only increased significantly under WT fluctuation treatments. SOC contents rose under waterlogged conditions but declined with WT fluctuation. Both dissolved organic carbon (DOC) and microbial biomass carbon were elevated in surface soils subjected to waterlogged and WT fluctuation compared to those in drained peat. However, the easily oxidizable carbon contents were only elevated in surface soils subjected to waterlogged treatments compared to those in drained peat. Soil moisture content had the greatest positive influence, while pH had the greatest negative influence on SOC contents. Actinomycete and DOC had negative impacts on SOC contents under varied WT conditions. This study clarifies the regulatory influence of WT on microbial community dynamics and enzyme activities relevant to SOC storage in degraded peatlands, highlighting the importance of WT management for effective peatland restoration.

泥炭地是全球碳循环的重要储存库；然而，广泛的退化导致土壤有机碳（SOC）含量显著降低。复湿对退化泥炭地活性有机碳的影响及其潜在的微生物机制尚不完全清楚。通过控制地下水位（WT）的方法，研究了长白山干枯土壤中完整泥炭柱的有机碳含量、活性有机碳含量、微生物群落组成和酶活性的变化。结果表明，WT的变化对土壤表层微生物生物量和酶活性有显著影响。涝渍导致细菌和放线菌总生物量下降，真菌生物量显著增加，尤其是在WT波动下。土壤氧化酶和水解酶活性仅在WT波动处理下显著增加。土壤有机碳含量在淹水条件下呈上升趋势，但随WT波动而下降。受涝渍和WT波动影响的表层土壤溶解有机碳（DOC）和微生物生物量碳均高于排水泥炭。然而，与排水泥炭相比，淹水处理仅使表层土壤的易氧化碳含量升高。土壤含水量对有机碳含量的正向影响最大，pH对有机碳含量的负向影响最大。放线菌和DOC对土壤有机碳含量有负向影响。本研究阐明了WT对退化泥炭地微生物群落动态和与有机碳储量相关的酶活性的调控作用，强调了WT管理对有效恢复泥炭地的重要性。

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引用次数: 0

Interplanting Phoebe bournei modifies soil microbial community characteristics in Cunninghamia lanceolata monocultures 间作杉木对杉木单作土壤微生物群落特征的影响

IF 3.3 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2025-09-01 DOI: 10.1016/j.ejsobi.2025.103762

Dan Zhou , Chengjian Hong , Jiahuan Guo , Chang Pan , Yazhou Tang , Jie Yan , Kaizhi Xie , Yuanchun Yu

The prolonged monoculture of Cunninghamia lanceolata depletes soil fertility, making the introduction of broadleaf trees—Phoebe bournei indispensable for soil restoration—yet its impact on the belowground ecological environment remains inadequately explored. Here, we investigated how uneven-aged interplanting of P. bournei in subtropical C. lanceolata stands alters microbial community characteristics and correlates with major edaphic variables across 0–60 cm soil profile. Interplanting P. bournei improved soil aeration, water conservation, and nutrient availability, driving significant shifts in bacterial and fungal β-diversity and partial but significant changes in community composition. It also enhanced ecological drift and reduced dispersal limitation, strengthening homogeneous selection in bacterial communities (0–40 cm) while promoting drift in fungal communities (0–60 cm). These structural and assembly changes suggest potential functional alterations in organic-matter degradation and nutrient cycling. Interplanting P. bournei led to marked improvements in soil microbial ecology, as evidenced by higher abundances of Acidobacteria and Ascomycota, indicative of enhanced complex-carbon degradation; elevated bacterial cellulolytic capacity and accelerated organic-matter turnover; promotion of saprotroph–symbiotroph nutrition; and reduced subsoil pathotroph abundance, which may indirectly support C. lanceolata health. Lastly, soil nutrient elements (e.g., soil organic carbon, total phosphorus) and enzyme activities (e.g., Cellobiohydrolase, Sucrase) were identified as key drivers of microbial community structure and functional potential, highlighting their critical roles in shaping soil microbial ecosystems. In summary, uneven-aged interplanting P. bournei in C. lanceolata plantations optimized soil ecosystem functions, offering a sustainable strategy to enhance forest productivity and improve soil health.

杉木的长期单一栽培消耗了土壤肥力，因此引入阔叶树——杉木是恢复土壤不可缺少的手段，但其对地下生态环境的影响尚未得到充分的探讨。本文以亚热带杉木林为研究对象，研究了不均匀套种林龄对微生物群落特征的影响及其与0 ~ 60 cm土壤剖面主要土壤变量的关系。套种柏树改善了土壤的通气性、保水性和养分有效性，显著改变了细菌和真菌的β-多样性和部分但显著的群落组成。它还增强了生态漂变，减少了扩散限制，加强了细菌群落（0-40 cm）的同质选择，同时促进了真菌群落（0-60 cm）的漂变。这些结构和组装的变化表明有机物降解和养分循环的潜在功能改变。土壤中酸菌群和子囊菌群的丰度显著提高，表明复合碳降解能力增强；提高细菌的纤维素分解能力和加速有机物的周转；腐养-共生营养促进；土壤病原菌丰度降低，可能间接支持杉木的健康。最后，土壤养分元素（如土壤有机碳、全磷）和酶活性（如纤维素生物水解酶、蔗糖酶）被认为是微生物群落结构和功能潜力的关键驱动因素，强调了它们在塑造土壤微生物生态系统中的关键作用。综上所述，间作杉木优化了土壤生态系统功能，为提高森林生产力和改善土壤健康提供了可持续的策略。

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引用次数: 0

Mechanisms of furrow-applied biochar in enhancing rhizosphere soil microbiota and metabolites in continuous sesame cropping 沟施生物炭提高芝麻连作根际土壤微生物群和代谢物的机制

IF 3.3 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2025-08-27 DOI: 10.1016/j.ejsobi.2025.103763

Ruiqing Wang , Fengjuan Lyu , Rujie Lyu , Junhai He , Lingen Wei

Biochar application significantly reduces bacterial wilt and boosts yield in continuous sesame cropping, but high costs limit its widespread use. This study used metagenomic and metabolomic analyses to evaluate three biochar application strategies (surface [BR3, 15,000 kg ha⁻¹], furrow [BR4, 1,875 kg ha⁻¹], and no biochar [BR0]) across four sample types (D0/D1: diseased; H0/H1: healthy; rhizosphere/non-rhizosphere), analyzing their effects on microbial community as well as their functional and metabolic profiles in sesame soil. The results showed that BR4 significantly increased sesame yield by 26.57 % compared to the control (BR0). Biochar treatments (BR3/BR4) enhanced the relative abundance of Actinomycetota while suppressing Pseudomonas in rhizosphere and non-rhizosphere soils of diseased plants. Sphingobium amiense and S. indicum were differentially abundant in BR3H0 and BR4H0, respectively. We observed disruption of fatty acid metabolism in BR3D0, which was linked to growth inhibition in continuously cropped sesame. BR4H0 treatment significantly increased the relative abundance of pyruvate dehydrogenase and the superpathway for de novo biosynthesis of pyrimidine ribonucleotides. The rhizospheres treated with BR4 from healthy plants showed higher levels of C17-sphinganine (+2.79 percentage points) and 6-[3-(dihydroxyphenyl-methyl)-methoxybutyl]-benzodioxole (+0.18 percentage points) compared to diseased plants. Partial Least Squares Discriminant Analysis (PLS-DA) identified Jasmine lactone and Tris(hydroxymethyl)aminomethane as key metabolite discriminators under positive ion mode, as opposed to Taurochenoxycholate and Palmitic acid under negative ion mode. These findings suggest that furrow-applied biochar at a reduced rate (1,875 kg ha⁻¹) can enhance soil microbial structure, function, and metabolite composition, ultimately improving yield in continuous sesame cropping systems.

在芝麻连作中施用生物炭可显著减少青枯病，提高产量，但成本高限制了其广泛应用。本研究利用宏基因组学和代谢组学分析，在4种样品类型（D0/D1：患病；H0/H1：健康；根际/非根际）中评估了3种生物炭施用策略（地表[BR3, 15,000 kg ha−1]，垄作[BR4, 1,875 kg ha−1]和无生物炭[BR0]），分析了它们对芝麻土微生物群落的影响以及它们的功能和代谢特征。结果表明，与对照（BR0）相比，BR4显著提高了26.57%的芝麻产量。生物炭处理（BR3/BR4）提高了病株根际和非根际土壤放线菌的相对丰度，抑制了假单胞菌。amiense和S. indicum的BR3H0和BR4H0含量差异较大。我们观察到BR3D0脂肪酸代谢的破坏，这与连作芝麻的生长抑制有关。BR4H0处理显著增加了丙酮酸脱氢酶的相对丰度和嘧啶核糖核苷酸从头生物合成的超途径。经BR4处理的健康植株根际中c17 -鞘氨氨酸（+2.79个百分点）和6-[3-（二羟基苯基-甲基）-甲氧基丁基]-苯二氮唑（+0.18个百分点）含量高于患病植株。偏最小二乘判别分析（PLS-DA）发现，茉莉内酯和三羟甲基胺甲烷在正离子模式下是关键的代谢物判别因子，而牛磺酸氧胆酸和棕榈酸在负离子模式下是关键的代谢物判别因子。这些结果表明，在芝麻连作系统中，以减量施用生物炭（1875 kg ha - 1）可以改善土壤微生物结构、功能和代谢物组成，最终提高产量。

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引用次数: 0

Investigating soil trophic links in a peatland, northeast China: Dual stable isotope analysis (δ13C and δ15N) of microarthropods and their food sources 东北泥炭地土壤营养联系研究：微节肢动物及其食物来源的δ13C和δ15N双稳定同位素分析

IF 3.3 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2025-08-05 DOI: 10.1016/j.ejsobi.2025.103761

Dandan Liu , Yiling Lin , Haitao Wu

Peatlands are crucial global carbon sinks and key players in carbon cycles, with their soil food webs supporting vital ecosystem processes. However, trophic relationships and carbon source uses of soil microarthropods in peatlands are poorly understood, limiting our understanding of their role in material cycling and ecosystem stability. Here, we employed dual stable isotope analysis (δ¹³C and δ¹⁵N) to investigate the trophic structure and carbon source utilization of soil microarthropods (Collembola, Oribatida, and Mesostigmata) in the Hani Peatland of northeastern China. Our results reveal clear trophic differentiation among taxa, with Mesostigmata occupying higher positions and Oribatida displaying broader isotopic niches. Although Collembola exhibited a weak correlation between body mass and isotope values, this pattern was not consistent across taxa. Mosses, particularly Polytrichum strictum, contributed significantly to the carbon intake of Collembola and Oribatida. However, δ¹³C enrichment patterns suggest that carbon transfer is primarily mediated through moss-associated microbial pathways rather than direct moss consumption. These findings provide novel insights into the structure and functioning of peatland soil food webs and highlight the ecological importance of microarthropods in belowground biogeochemical cycling. Understanding their roles is critical for predicting peatland responses to environmental change and informing conservation strategies.

泥炭地是至关重要的全球碳汇和碳循环的关键参与者，其土壤食物网支持重要的生态系统过程。然而，泥炭地土壤微节肢动物的营养关系和碳源利用尚不清楚，限制了我们对其在物质循环和生态系统稳定中的作用的理解。本文采用双稳定同位素（δ13C和δ15N）分析了哈尼泥炭地土壤微节肢动物（弹虫、甲虫和中刺虫）的营养结构和碳源利用。我们的研究结果揭示了类群间明显的营养分化，中柱头甲占据较高的位置，甲骨甲显示出更广泛的同位素生态位。虽然弹虫的体质量与同位素值的相关性较弱，但这种模式在不同的分类群中并不一致。苔藓类植物对弹虫和甲虫的碳吸收有显著的促进作用，其中以窄曲多毛虫最为显著。然而，δ13C富集模式表明碳转移主要是通过苔藓相关的微生物途径介导的，而不是直接消耗苔藓。这些发现为泥炭地土壤食物网的结构和功能提供了新的见解，并强调了微节肢动物在地下生物地球化学循环中的生态重要性。了解它们的作用对于预测泥炭地对环境变化的反应和为保护策略提供信息至关重要。

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引用次数: 0

Crop rotation and organic fertilizer maintains diversity and activity of cbbL-carrying CO2-fixing bacteria in reclaimed coal mining soils 轮作和有机肥可维持复垦煤矿土壤中携带cbbl的co2固定细菌的多样性和活性

IF 3.3 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2025-07-31 DOI: 10.1016/j.ejsobi.2025.103759

Shuning Bai , Meihua Fan , Min Wu , Xiaolong Sui , Yibo Song , Yunlong Jiang , Huisheng Meng , Yulin Liu , Xiangying Wang , Xianjun Hao , Yi Li , Jianping Hong , Jie Zhang

CO₂-fixing bacteria are an important factor in restoring soil health in coal mining areas. The impact of crop rotation and fertilization on CO₂-fixing bacteria in reclaimed mining soils remains unclear. To narrow this knowledge gap, in this study, maize (Zea mays L.) monoculture (M) and maize-soybean (Glycine max) rotation (R) cropping systems were set up in a coal-mining reclamation area with four fertilization treatments in each, namely, CK (without fertilization), inorganic fertilizer (F), organic fertilizer (O), and combined organic and inorganic fertilizer (OF). The abundance, diversity, community composition and RubisCO activity of CO₂-fixing bacteria in topsoil under those treatments were investigated respectively using quantitative PCR, high-throughput sequencing based on the cbbL gene [that encodes ribulose-1,5-biphosphate carboxylase/oxygenase (RubisCO)] and enzyme-linked immunosorbent assay (ELISA). The results showed that R_O significantly increased easily oxidized organic carbon (EOC), total nitrogen (TN), and available nitrogen (AN) (P < 0.05). It also significantly increased the biomass of CO₂-fixing bacteria (P < 0.05) and altered the CO₂-fixing bacterial community. The CO₂-fixing bacteria in R_OF, R_O and M_O exhibited comparable community structures and harbored a greater co-occurrence network complexity than other treatments. Several CO₂-fixing bacteria associated with nitrogen cycling, such as Devosia, Nitrobacter, Hyphomicrobiales and Nitrosospira, were significantly enriched under the maize-soybean rotation system (P < 0.05). This study implied that crop rotation and organic fertilizer application could synergistically foster soil quality restoration in coal mining area by elevating soil nutrients and maintaining biomass, diversity and community structure of cbbL-carrying CO₂-fixing bacteria, establishing a theoretical foundation for optimizing carbon sequestration strategies in post-mining ecological rehabilitation.

固结菌是恢复矿区土壤健康的重要因子。轮作和施肥对复垦矿区土壤中固定co2细菌的影响尚不清楚。为了缩小这方面的知识差距，本研究在某煤矿垦区建立了玉米（Zea mays L.）单作(M)和玉米-大豆（Glycine max）轮作(R)制度，每种施肥处理分别为CK（不施肥）、无机肥(F)、有机肥(O)和有机无机复合肥（OF）。采用定量PCR、基于cbbL基因（编码核酮糖-1,5-二磷酸羧化酶/加氧酶（RubisCO））的高通量测序和酶联免疫吸附试验（ELISA）分别研究了不同处理下表层土壤co2固定菌的丰度、多样性、群落组成和RubisCO活性。结果表明：R_O显著提高了易氧化有机碳（EOC）、总氮（TN）和速效氮（AN） (P <；0.05)。它还显著增加了co2固定菌的生物量(P <；0.05)，并改变了固定co2的细菌群落。与其他处理相比，R_OF、R_O和M_O的co2固定菌群落结构相似，共发生网络复杂性更高。玉米-大豆轮作制度下，与氮循环相关的几种co2固定菌，如Devosia、Nitrobacter、菌丝微生物和亚硝基螺旋体显著富集(P <；0.05)。本研究表明，轮作与有机肥施用可通过提高土壤养分、维持携带cbbl的co2固定菌的生物量、多样性和群落结构，协同促进矿区土壤质量恢复，为优化采煤后生态恢复固碳策略奠定理论基础。

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引用次数: 0

Multifunctional endophytic bacteria intimately associated within spores of arbuscular mycorrhizal fungi in a chernozem soil in Central Europe 在中欧黑钙土中与丛枝菌根真菌孢子密切相关的多功能内生细菌

IF 3.3 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2025-07-30 DOI: 10.1016/j.ejsobi.2025.103760

Helmut Baltruschat , Johanna Hummel , Marit Gillmeister , Stefan Ratering , Kathrin Kabrodt , Ewald Sieverding , Fritz Oehl

Chernozems are counted among the most fertile soils worldwide. Unexpectedly high spore density and species richness of arbuscular mycorrhizal fungi (AMF) were found in a long-term field trial established on such a soil. The purpose of the present study was to estimate bacterial communities associated within spores of selected AMF species from a long-term field trial on a highly fertile Calcic Chernozem to unravel their diversity belonging to different genera and species. We hypothized that high AMF species richness found in the Chernozem soil is reflected in a bacterial diversity with multifunctional traits mediated by indigenous bacterial compositions. The AMF species Funneliformis mosseae, Scutellospora calospora and Septoglomus nigrum were selected, since they occurred abundantly both in reduced and conventional tillage systems. The pure cultures of isolated bacterial strains were tested for ecological functions (traits) such as phosphorus solubilization, siderophore production, indole-3-acetic acid production and 1-aminocyclopropane-1-carboxylate deaminase activity. In addition, antimicrobial activity against both hemibiotrophic and necrotrophic fungi and oomycetes was evaluated. The majority of bacterial strains was exclusively associated with only one of the three AMF species, thus, giving evidence that each AMF species may harbor its own bacterial community. A large number of bacterial communities was shown to exert multifunctional activities ranging from plant growth promotion traits to antimicrobial activity. These findings suggest that the multifunctionality of bacteria intimately associated with AMF could markedly expand the ecological function of an autochthonous AMF population and empower host plants to explore robust ways to cope with changing environmental conditions.

黑钙土是世界上最肥沃的土壤之一。在这种土壤上进行了长期的田间试验，发现丛枝菌根真菌（AMF）的孢子密度和物种丰富度出乎意料地高。本研究的目的是通过在高肥力钙质黑钙土上进行的长期田间试验，估计选定的AMF物种孢子内的细菌群落，以揭示它们属于不同属和物种的多样性。我们假设黑钙土中AMF物种丰富度高反映在原生细菌组成介导的多种功能特征的细菌多样性上。选择的AMF种有：mossefuneliformis、calospora Scutellospora和Septoglomus nigrum，因为它们在减量耕作和常规耕作制度中都大量存在。对分离菌株的纯培养物进行了磷增溶、铁载体产量、吲哚-3-乙酸产量和1-氨基环丙烷-1-羧酸脱氨酶活性等生态功能（性状）的测试。此外，还评估了其对半生物营养和坏死性真菌及卵菌的抑菌活性。大多数菌株只与三种AMF中的一种相关，因此，证据表明每种AMF可能都有自己的细菌群落。大量的细菌群落被证明具有多种功能，从植物生长促进性状到抗菌活性。这些发现表明，与AMF密切相关的细菌的多功能性可以显着扩展本地AMF种群的生态功能，并使寄主植物能够探索应对不断变化的环境条件的强大方法。

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引用次数: 0

Ant-isopod interactions: spanning predation, facilitation, and myrmecophily 反等足类动物的相互作用：跨越捕食、促进和蚁寄生

IF 3.7 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2025-07-16 DOI: 10.1016/j.ejsobi.2025.103753

Thomas Parmentier , Stefano Taiti , Pallieter De Smedt

Ants and terrestrial isopods are two key groups within soil ecosystems both in terms of their biomass and the vital functions they contribute to. Despite the ecological importance of both groups and their frequent interactions, the dynamics between ants and terrestrial isopods remain poorly understood. We provide a systematic overview and discuss the diverse spectrum of ant-isopod interactions ranging from predation, through facilitation, to myrmecophily.

A limited number of ant species, restricted to the genus Leptogenys, specialize in preying on isopods, while a very broad group of ants opportunistically include them in their diet. To evade ant predation, terrestrial isopods employ defensive strategies such as avoidance, conglobation or secretion of repellent substances. Apart from the direct negative impacts of predation, ants impose several non-lethal effects on terrestrial isopods, influencing their spatial distribution, behaviour, and fitness. Invasive ants may, in contrast, indirectly benefit isopods by feeding on their predators or competitors. A diverse group of isopods are not deterred by ants and reside in their nests. These myrmecophilous isopods vary from facultative associates, which cohabit with ants only occasionally and are represented by many records, to obligate guests that live permanently within ant nests. These obligate guests thrive in ant fortresses by specific behavioural, chemical and morphological adaptations. Their effect on the host can range from negative to neutral, or potentially even beneficial. Though modest in number, with 23 species across 10 genera, the diversity of obligate myrmecophilous isopods showcases a fascinating pattern of independent evolution in soil ecosystems.

蚂蚁和陆生等足类动物是土壤生态系统中的两个关键类群，无论是从它们的生物量还是它们所起的重要作用来看都是如此。尽管蚂蚁和陆生等足类动物在生态上都很重要，而且它们之间的互动也很频繁，但人们对它们之间的动态关系仍然知之甚少。我们提供了一个系统的概述，并讨论了各种各样的抗等足类动物的相互作用，从捕食，通过促进，到食蚁兽。数量有限的蚂蚁种类，仅限于轻足类，专门捕食等足类动物，而非常广泛的蚂蚁群体则机会主义地将它们纳入其饮食中。为了躲避蚂蚁的捕食，陆生等足类动物采用诸如躲避、聚集或分泌驱避物质等防御策略。除了捕食的直接负面影响外，蚂蚁还对陆地等足类动物施加了一些非致命的影响，影响了它们的空间分布、行为和适应性。相比之下，入侵蚂蚁可能通过捕食它们的捕食者或竞争对手，间接地使等足类动物受益。一群不同的等足类动物不会被蚂蚁吓倒，而是住在蚂蚁的巢穴里。这些嗜myrmecophilous等足类动物从偶尔与蚂蚁同居的兼性伙伴（有许多记录）到永久居住在蚂蚁巢穴中的义务客人（有许多记录）不等。这些有义务的客人通过特定的行为、化学和形态适应在蚂蚁堡垒中茁壮成长。它们对宿主的影响从负面到中性，甚至可能是有益的。虽然数量不多，只有10属23种，但专性嗜蜜等足类动物的多样性展示了土壤生态系统中独立进化的迷人模式。

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引用次数: 0

Deciphering the microbial mechanisms underlying glucose induced soil priming effects under low and high nutrient levels 解读低、高营养水平下葡萄糖诱导土壤启动效应的微生物机制

IF 3.7 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2025-07-16 DOI: 10.1016/j.ejsobi.2025.103757

Jiaqi Wang , Xichang Zhang , Yingyi Fu , Lukas Van Zwieten , Han Sun , Georg Guggenberger , Lingfei Hu , Yu Luo , Tida Ge , Yakov Kuzyakov

Soil priming effect, which refers to the impact of labile carbon inputs on the decomposition of soil organic matter, plays a significant role in carbon storage. Investigating substrate induced soil priming effects and the involved microbial mechanisms, particularly under nutrients gradient, is central to the understanding of carbon processes and potential accrual in agriculture soil. Thus, a 7 days laboratory incubation was conducted to assess ¹³C labeled glucose induced priming effects in soil receiving the high (TH) and low nutrient (TL) addition. Also, DNA-SIP coupled with metagenomic were adopted to identify the core microbial groups and functional guild responsible for soil priming effects. Here, we found that i) soil priming effects were significantly larger in TL treatment than in the TH treatment, and ii) the larger priming in TL was likely driven by N-mining processes dominated by K-strategy microbes, whereas less priming in TH might be explained by co-metabolism led by r-strategy microbes. Additionally, functional changes of microbial community were revealed by Shotgun sequencing. Both KEGG, EggNOG and CAZymes showed the relative abundance of the functional genes (e.g., GH13_10 and GH77) encoding cellulase enzymes involved in soil organic carbon decomposition were more abundant in TL compared to TH, suggesting higher priming effects in TL was mainly due to the nutrient constraints on microbial demands. This study revealed the main microbial groups and their functions in glucose induced soil priming effects under low and high nutrient levels.

土壤启动效应是指不稳定碳输入对土壤有机质分解的影响，在碳储量中起着重要作用。研究基质诱导的土壤启动效应和相关的微生物机制，特别是在养分梯度下，是理解农业土壤中碳过程和潜在积累的核心。因此，通过7天的实验室培养来评估13C标记葡萄糖在土壤中添加高（TH）和低营养（TL）诱导的启动效应。同时，采用DNA-SIP结合宏基因组技术鉴定土壤启动效应的核心微生物群和功能区系。本研究发现：1)TL处理的土壤启动效应明显大于TH处理；2)TL中较大的土壤启动效应可能是由k策略微生物主导的n挖掘过程驱动的，而TH中较少的土壤启动效应可能是由r策略微生物主导的共代谢引起的。此外，通过霰弹枪测序分析了微生物群落的功能变化。KEGG、EggNOG和CAZymes均显示，与TH相比，TL中编码土壤有机碳分解纤维素酶的功能基因（如GH13_10和GH77）的相对丰度更高，说明TL中较高的启动效应主要是由于养分对微生物需求的限制。本研究揭示了低、高营养水平下葡萄糖诱导土壤启动效应的主要微生物类群及其功能。

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引用次数: 0

Pedogenesis of volcanic ash soils determines the soil microbial pool and its contribution to nutrient availability 火山灰土的成土作用决定了土壤微生物库及其对养分有效性的贡献

IF 3.7 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2025-07-12 DOI: 10.1016/j.ejsobi.2025.103756

John Clunes , Rebecca Hood-Nowotny , Celia Férnandez-Balado , Susan Valle , Sabine Huber , Ferdinand Hartmann , Niklas Bruhn , Christoph Rosinger , Dante Pinochet , Lorena Lagos , Katharina Keiblinger

Pedogenesis is a key factor driving nutrient immobilization and mineralization, particularly relevant in the fertility management of volcanic soils. These processes have a strong effect on plant production, but also on the functionality of a soil microbial pool. This research aimed to determine the effect of substrate addition on soil microbial pool, substrate use and nitrogen availability by performing a double labeling study (¹³C and ¹⁵N isotope labeling) in five different volcanic ash soils under permanent pastures. Disturbed soil samples were collected in five volcanic ash soils between the Andes and Coastal Mountains of Chile to evaluate contrasting pedogenesis. Soils were incubated for 16 days at 20 °C and a 60 % water holding capacity. Destructive sampling was carried out on days 1, 2, 3, 6, 9 and 16. Labeling was tracked through the consumption by the microbial biomass, functional genes, stoichiometric imbalances between microbial C/N, enzymatic C/N and the release of CO₂ during the incubation period. Ultisol and well-developed Andisol (“Older soils”) with higher organic matter content (∼14 %) showed higher available ¹⁵N contents, higher ¹³C-CO₂ respiration, and immobilization of substrate into microbial biomass (i.e., ¹³C-MBC). This study found that depending on the pedogenesis of the soil, N availability from mineral N and N-acquiring enzyme activities changed as a function of a microbial pool inherent in each soil. Thus, the effect of the initial activity of the soil microbial pool on N availability will allow adjusting fertilization strategies in soils with high levels of organic matter such as volcanic ash soils.

成土作用是驱动养分固定和矿化的关键因素，尤其与火山土壤的肥力管理有关。这些过程对植物生产有很强的影响，但也对土壤微生物库的功能有很大的影响。本研究通过对5种不同永久牧场下的火山灰土壤进行13C和15N同位素双标记研究，以确定添加基质对土壤微生物库、基质利用和氮有效性的影响。在智利安第斯山脉和海岸山脉之间的五个火山灰土壤中收集了扰动土壤样品，以评估对比的土壤形成过程。土壤在20°C和60%保水能力条件下培养16天。在第1、2、3、6、9和16天进行破坏性采样。通过微生物生物量的消耗、功能基因、微生物C/N、酶C/N之间的化学计量失衡以及孵化期间二氧化碳的释放来跟踪标记。有机物质含量较高（~ 14%）的Ultisol和发育良好的andiol（“老土壤”）显示出更高的有效15N含量，更高的13C-CO2呼吸，以及将底物固定为微生物生物量（即13C-MBC）。本研究发现，根据土壤的成土作用，矿质氮和氮获取酶活性的氮有效性随每种土壤中固有的微生物库的功能而变化。因此，土壤微生物库的初始活性对氮有效性的影响将有助于调整有机质含量高的土壤（如火山灰土壤）的施肥策略。

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引用次数: 0

Changes in carbon and nitrogen stable isotopic ratios with decomposition of plant residue 植物残体分解过程中碳氮稳定同位素比值的变化

IF 3.7 2区农林科学 Q1 ECOLOGY

European Journal of Soil Biology

Pub Date : 2025-07-09 DOI: 10.1016/j.ejsobi.2025.103755

Xiaodong Chen , Ryoko Senda , Yuki Mori , Syuntaro Hiradate

The transformation of plant residue into soil organic matter (SOM) plays a crucial role in maintaining the function of ecosystems. To elucidate the formation processes of SOM from plant residue, we examined changes in carbon (C) and nitrogen (N) contents, along with δ¹³C and δ¹⁵N values, during the decomposition of Artemisia princeps residue in an incubation experiment using 100 % quartz sand as an artificial soil. The results indicated that plant residue decomposition occurred in two distinct stages: (1) a rapid initial loss of C and N (43–54 % and 36–47 %, respectively) within the early 90 days, followed by (2) a slower loss (4–14 % and 7–15 %, respectively) over the remaining 90–360 days. In the early stage, δ¹³C and δ¹⁵N values increased similarly to trophic fractionation, with a δ¹⁵N/δ¹³C ratio of 1.54. In the latter stage, this ratio increased significantly to 12.8, probably due to increased C resistance to decomposition, while continuous N metabolism. By integrating data from both the literature and our study, we concluded that C and N in plant residue undergo one to two times the metabolic turnover relative to trophic turnover to form stable SOM. These findings are essential for understanding the preservation of plant residue, particularly labile compounds, in soils. This preservation would occur through microbial transformation. Our study offers a theoretical framework for understanding the SOM dynamics based on δ¹³C and δ¹⁵N values, emphasizing the utility of stable C and N isotope analyses in elucidating SOM transformation.

植物残茬向土壤有机质的转化对维持生态系统的功能起着至关重要的作用。为了阐明植物残渣中SOM的形成过程，我们采用100%石英砂作为人工土壤，研究了青蒿残渣分解过程中碳(C)和氮(N)含量以及δ13C和δ15N值的变化。结果表明，植物残体分解分两个阶段：(1)初90 d内C和N的快速损失（分别为43 - 54%和36 - 47%），随后(2)90 - 360 d内C和N的缓慢损失（分别为4 - 14%和7 - 15%）。早期δ13C和δ15N值的增加与营养分馏相似，δ15N/δ13C比值为1.54。在后期，这一比例显著增加到12.8，可能是由于C对分解的抵抗力增强，同时N的代谢持续进行。通过综合文献数据和我们的研究，我们得出结论，植物残体中的C和N相对于营养周转要经历一到两倍的代谢周转才能形成稳定的SOM。这些发现对于了解植物残留物，特别是不稳定化合物在土壤中的保存是至关重要的。这种保存将通过微生物转化来实现。我们的研究为理解基于δ13C和δ15N值的SOM动力学提供了理论框架，强调了稳定C和N同位素分析在阐明SOM转变中的作用。

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引用次数: 0