Pub Date : 2025-10-15DOI: 10.1016/j.fbp.2025.10.014
Eduarda Caetano Peixoto , Elder Pacheco da Cruz , Tatiane Jéssica Siebeneichler , Laura Martins Fonseca , Julia Eisenhardt de Mello , Roselia Maria Spanevello , Alvaro Renato Guerra Dias , Elessandra da Rosa Zavareze , Cesar Valmor Rombaldi
Pecan nut shells are typically discarded as waste. However, their valorization holds significant potential due to the presence of phenolic compounds and antioxidant properties. This study sought to enhance the value of this byproduct by developing cryogels derived from pea starch, enriched with pecan nut shell extract (PNSE). Develop a fully biodegradable material suitable for food packaging that is capable of incorporating bioactive compounds. The cryogels were prepared by gelatinizing the starch, followed by physical crosslinking through five cycles of freezing and thawing. PNSE was incorporated into the cryogels at 10 % and 20 % (w/w). PNSE was evaluated for its individual phenolic compounds and cytotoxicity. The cryogels were assessed for their morphology, density, porosity, water absorption, hardness, antioxidant activity, reuse potential, and biodegradability. PNSE contained phenolic compounds, with tannins and procyanidins identified as the major groups. These compounds are primarily responsible for the bioactivity of PNSE. The cryogels exhibited a macroporous and homogeneous morphology. All cryogels had a low density (0.058–0.078 g/cm³), high porosity (88–91 %), and high water absorption, reaching up to 1513 %. The incorporation of PNSE increased the hardness of the cryogels, thereby enhancing their structural integrity. Cryogels demonstrated reusability over 15 cycles, with 600 % absorption potential. All cryogels were biodegradable in soil within 15 days. These cryogels represent effective matrices with potential applications as water absorbers for fresh food products that release exudates, as well as carriers of antioxidant and bioactive compounds.
{"title":"Biodegradable cryogels based on pea starch as carriers of bioactive compounds from pecan (Carya illinoinensis) co-product: Synthesis, characterization, and prospecting for technological use","authors":"Eduarda Caetano Peixoto , Elder Pacheco da Cruz , Tatiane Jéssica Siebeneichler , Laura Martins Fonseca , Julia Eisenhardt de Mello , Roselia Maria Spanevello , Alvaro Renato Guerra Dias , Elessandra da Rosa Zavareze , Cesar Valmor Rombaldi","doi":"10.1016/j.fbp.2025.10.014","DOIUrl":"10.1016/j.fbp.2025.10.014","url":null,"abstract":"<div><div>Pecan nut shells are typically discarded as waste. However, their valorization holds significant potential due to the presence of phenolic compounds and antioxidant properties. This study sought to enhance the value of this byproduct by developing cryogels derived from pea starch, enriched with pecan nut shell extract (PNSE). Develop a fully biodegradable material suitable for food packaging that is capable of incorporating bioactive compounds. The cryogels were prepared by gelatinizing the starch, followed by physical crosslinking through five cycles of freezing and thawing. PNSE was incorporated into the cryogels at 10 % and 20 % (w/w). PNSE was evaluated for its individual phenolic compounds and cytotoxicity. The cryogels were assessed for their morphology, density, porosity, water absorption, hardness, antioxidant activity, reuse potential, and biodegradability. PNSE contained phenolic compounds, with tannins and procyanidins identified as the major groups. These compounds are primarily responsible for the bioactivity of PNSE. The cryogels exhibited a macroporous and homogeneous morphology. All cryogels had a low density (0.058–0.078 g/cm³), high porosity (88–91 %), and high water absorption, reaching up to 1513 %. The incorporation of PNSE increased the hardness of the cryogels, thereby enhancing their structural integrity. Cryogels demonstrated reusability over 15 cycles, with 600 % absorption potential. All cryogels were biodegradable in soil within 15 days. These cryogels represent effective matrices with potential applications as water absorbers for fresh food products that release exudates, as well as carriers of antioxidant and bioactive compounds.</div></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":"154 ","pages":"Pages 475-488"},"PeriodicalIF":3.4,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145358041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alfalfa is gaining interest as an alternative protein source. The European Food Safety Authority (EFSA) approval of alfalfa protein concentrate as novel food states presence of metabolites as a barrier for higher amount of consumption. A promising method for protein isolation is anion exchange chromatography, which is scalable through expanded bed chromatography. In this study, ultrafiltration-buffer exchange (UBE) and ultrafiltration-diafiltration was applied as an upstream and downstream processing to anion exchange chromatography, respectively. The performance of these techniques was compared to that of lab scale dilution and centrifugal spin filters. The result showed that UBE shortened the chromatography duration by 50 % and resulted in a cleaner column. However, downstream desalination through ultrafiltration-diafiltration resulted in concentrate with lower protein content (60.41 ± 0.84 %), compared to that obtained from centrifugal spin filters (90.49 ± 1.34 %), suggesting the need to carry out extra diafiltration steps. Untargeted metabolomics revealed that high absorption at 214 nm in the anion exchange flowthrough fraction could be attributed to di- and tripeptides also present in the ultrafiltration permeates. Polyphenols present in ultrafiltration permeate were absent from both flowthrough and 225 mM fraction, suggesting that they might bind strongly to the column. Various carboxylic acids were present in the ultrafiltration permeate, chromatography flowthrough, and 225 mM NaCl fraction. Contaminants eluting at 225 mM NaCl with high absorbance at 214 nm were not successfully identified although their occurrence was lower in UBE samples. These results suggest that UBE is promising as pre-treatment for purification of RuBisCO from alfalfa using anion exchange chromatography.
苜蓿作为一种替代蛋白质来源正引起人们的兴趣。欧洲食品安全局(EFSA)批准苜蓿蛋白浓缩物作为新型食品,指出其代谢物的存在是大量食用的障碍。阴离子交换色谱法是一种很有前途的蛋白质分离方法,它可以通过扩展床色谱法进行扩展。在本研究中,超滤-缓冲交换(UBE)和超滤-滤滤分别作为阴离子交换色谱的上游和下游处理。将这些技术的性能与实验室规模稀释和离心自旋过滤器的性能进行了比较。结果表明,UBE使色谱时间缩短了50% %,色谱柱更干净。然而,与离心自旋过滤器获得的浓缩物(90.49 ± 1.34 %)相比,下游通过超滤-滤除得到的浓缩物蛋白质含量(60.41 ± 0.84 %)较低,这表明需要进行额外的滤除步骤。非靶向代谢组学显示,在214 nm处阴离子交换流过部分的高吸收可能归因于超滤渗透物中也存在的二肽和三肽。超滤渗透液中存在的多酚在流过和225 mM馏分中均不存在,表明它们可能与柱结合强烈。超滤渗透液、色谱流和225 mM NaCl馏分中存在不同的羧酸。在225 mM NaCl洗脱的214 nm高吸光度的污染物虽然在UBE样品中的发生率较低,但未能成功鉴定。这些结果表明,UBE有望作为阴离子交换色谱法纯化紫花苜蓿中RuBisCO的前处理。
{"title":"Proof-of-concept: Ultrafiltration as a pre-treatment for the anion exchange chromatography of RuBisCO from alfalfa","authors":"Hartono Tanambell , Marianne Danielsen , Anders Hauer Møller , Trine Kastrup Dalsgaard","doi":"10.1016/j.fbp.2025.10.011","DOIUrl":"10.1016/j.fbp.2025.10.011","url":null,"abstract":"<div><div>Alfalfa is gaining interest as an alternative protein source. The European Food Safety Authority (EFSA) approval of alfalfa protein concentrate as novel food states presence of metabolites as a barrier for higher amount of consumption. A promising method for protein isolation is anion exchange chromatography, which is scalable through expanded bed chromatography. In this study, ultrafiltration-buffer exchange (UBE) and ultrafiltration-diafiltration was applied as an upstream and downstream processing to anion exchange chromatography, respectively. The performance of these techniques was compared to that of lab scale dilution and centrifugal spin filters. The result showed that UBE shortened the chromatography duration by 50 % and resulted in a cleaner column. However, downstream desalination through ultrafiltration-diafiltration resulted in concentrate with lower protein content (60.41 ± 0.84 %), compared to that obtained from centrifugal spin filters (90.49 ± 1.34 %), suggesting the need to carry out extra diafiltration steps. Untargeted metabolomics revealed that high absorption at 214 nm in the anion exchange flowthrough fraction could be attributed to di- and tripeptides also present in the ultrafiltration permeates. Polyphenols present in ultrafiltration permeate were absent from both flowthrough and 225 mM fraction, suggesting that they might bind strongly to the column. Various carboxylic acids were present in the ultrafiltration permeate, chromatography flowthrough, and 225 mM NaCl fraction. Contaminants eluting at 225 mM NaCl with high absorbance at 214 nm were not successfully identified although their occurrence was lower in UBE samples. These results suggest that UBE is promising as pre-treatment for purification of RuBisCO from alfalfa using anion exchange chromatography.</div></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":"154 ","pages":"Pages 440-448"},"PeriodicalIF":3.4,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145320437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-10DOI: 10.1016/j.fbp.2025.10.008
Zhaotian Yang , Jinghao Zhang , Suxia Shen , Ning Xu , Zixuan Han , Lin Zeng , Yan Zhang
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Chlorophyll (Chl), a natural pigment with multifunctional bioactive properties, faces limited industrial application due to its poor water solubility. In this study, we proposed a novel strategy utilizing Chl-rich spinach extracts (CRSE)—natural Chl carriers with inherent structural stability—to generate water-soluble Chl particles. CRSE contained high protein (47.57 %) and lipid (8.2 %) contents, enabling its use as a structural foundation. Sucrose fatty acid ester (SE) and maltodextrin (MD)-whey protein isolate (WPI) matrix were chosen to stabilize emulsions and generate CRSE-MD-WPI aggregation unit particles. The optimized formulation (1.5 % SE; 14 % wall materials, WPI:MD = 5:5) produced particles with enhanced encapsulation efficiency (81.75 %), solubility (86.83 %), and reduced moisture content (3.83 %). Structural analyses revealed that WPI established hydrophobic interactions with CRSE, hydrogen bonding with MD, and alterations both CRSE microenvironment polarity and MD crystallinity, thereby improving solubility and stability. Importantly, this approach reduced wall material usage by 50 % compared to conventional encapsulation. Overall, this work presents a scalable and eco-friendly protocol to enhance Chl solubility, providing a promising pathway for functional pigment applications in the food industry.
{"title":"A new strategy with forming the aggregation unit to improve chlorophyll water solubility based on its natural inherent structure carriers","authors":"Zhaotian Yang , Jinghao Zhang , Suxia Shen , Ning Xu , Zixuan Han , Lin Zeng , Yan Zhang","doi":"10.1016/j.fbp.2025.10.008","DOIUrl":"10.1016/j.fbp.2025.10.008","url":null,"abstract":"<div><div><span><figure><span><img><ol><li><span><span>Download: <span>Download high-res image (191KB)</span></span></span></li><li><span><span>Download: <span>Download full-size image</span></span></span></li></ol></span><span><span><p>Chlorophyll (Chl), a natural pigment with multifunctional bioactive properties, faces limited industrial application due to its poor water solubility. In this study, we proposed a novel strategy utilizing Chl-rich spinach extracts (CRSE)—natural Chl carriers with inherent structural stability—to generate water-soluble Chl particles. CRSE contained high protein (47.57 %) and lipid (8.2 %) contents, enabling its use as a structural foundation. Sucrose fatty acid ester (SE) and maltodextrin (MD)-whey protein isolate (WPI) matrix were chosen to stabilize emulsions and generate CRSE-MD-WPI aggregation unit particles. The optimized formulation (1.5 % SE; 14 % wall materials, WPI:MD = 5:5) produced particles with enhanced encapsulation efficiency (81.75 %), solubility (86.83 %), and reduced moisture content (3.83 %). Structural analyses revealed that WPI established hydrophobic interactions with CRSE, hydrogen bonding with MD, and alterations both CRSE microenvironment polarity and MD crystallinity, thereby improving solubility and stability. Importantly, this approach reduced wall material usage by 50 % compared to conventional encapsulation. Overall, this work presents a scalable and eco-friendly protocol to enhance Chl solubility, providing a promising pathway for functional pigment applications in the food industry.</p><div>Graphical abstract</div></span></span></figure></span></div></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":"154 ","pages":"Pages 371-378"},"PeriodicalIF":3.4,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145320440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-10DOI: 10.1016/j.fbp.2025.10.009
Yaxing Yang , Liyun Wu , Qingqing Zeng , Feifan Cao , Hu Shi , Juming Tang , Fanbin Kong , Zhe Wang , Yang Jiao
3D printing provides delicate and unique structure for low-salt surimi product development, but may require pretreatment methods for improving its stability and gel strength. This study investigated the effects of ultrasound pretreatments (400 W, 40 kHz, treatment time of 0–30 min) on the 3D printing and gelling properties of low-salt surimi. The results revealed that ultrasound pretreatment improved the surface smoothness of printed samples and decreased the filament breakage. The gel strength, water holding capacity and salt-soluble protein solubility of surimi gel initially increased with longer ultrasonic treatment times and then decreased, which indicated that appropriate ultrasound pretreatment promoted the interaction between protein molecules and water. As a result, more proteins participated in the formation of the gel network due to ultrasound application, which significantly enhanced the hardness and gel strength of the low-salt surimi gel. The change in total sulfhydryl content showed a contrary trend than the protein solubility, which might be attributed to more pronounced protein unfolding induced by ultrasound and subsequent exposure of -SH groups, leading to formation of more disulfide bonds. The low field NMR and MRI showed that the initial relaxation times were shortened and hydrogen proton density increased, indicating a tighter binding between protein and water and reduced water fluidity. Scanning electron microscope observations showed the surimi gel treated with ultrasound for 15 min formed a uniform and dense gel network. In conclusion, appropriate ultrasound pretreatment significantly improves the 3D printing and gelling properties of low-salt sliver carp surimi, which provides a promising method in low-salt surimi-based food development in 3D printing.
{"title":"Ultrasound pretreatment improves 3D printing and gelling properties of low-salt sliver carp (Hypophthalmichthys molitrix) surimi","authors":"Yaxing Yang , Liyun Wu , Qingqing Zeng , Feifan Cao , Hu Shi , Juming Tang , Fanbin Kong , Zhe Wang , Yang Jiao","doi":"10.1016/j.fbp.2025.10.009","DOIUrl":"10.1016/j.fbp.2025.10.009","url":null,"abstract":"<div><div>3D printing provides delicate and unique structure for low-salt surimi product development, but may require pretreatment methods for improving its stability and gel strength. This study investigated the effects of ultrasound pretreatments (400 W, 40 kHz, treatment time of 0–30 min) on the 3D printing and gelling properties of low-salt surimi. The results revealed that ultrasound pretreatment improved the surface smoothness of printed samples and decreased the filament breakage. The gel strength, water holding capacity and salt-soluble protein solubility of surimi gel initially increased with longer ultrasonic treatment times and then decreased, which indicated that appropriate ultrasound pretreatment promoted the interaction between protein molecules and water. As a result, more proteins participated in the formation of the gel network due to ultrasound application, which significantly enhanced the hardness and gel strength of the low-salt surimi gel. The change in total sulfhydryl content showed a contrary trend than the protein solubility, which might be attributed to more pronounced protein unfolding induced by ultrasound and subsequent exposure of -SH groups, leading to formation of more disulfide bonds. The low field NMR and MRI showed that the initial relaxation times were shortened and hydrogen proton density increased, indicating a tighter binding between protein and water and reduced water fluidity. Scanning electron microscope observations showed the surimi gel treated with ultrasound for 15 min formed a uniform and dense gel network. In conclusion, appropriate ultrasound pretreatment significantly improves the 3D printing and gelling properties of low-salt sliver carp surimi, which provides a promising method in low-salt surimi-based food development in 3D printing.</div></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":"154 ","pages":"Pages 415-425"},"PeriodicalIF":3.4,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145320435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-10DOI: 10.1016/j.fbp.2025.10.010
Xiaoxue Zhao , Yang Zhou , Caoxing Huang , Chenhuan Lai , Zhe Ling , Chunhui Liu , Qiang Yong
The polymerization degree of xylo-oligosaccharides is crucial in determining their prebiotic activity. Herein, soluble xylan with a moderate degree of polymerization (RX) and xylo-oligosaccharides enriched in xylobiose and xylotriose (XOS) were prepared from eucalyptus forestry residues. Their influence on gut microbiota and potential metabolic mechanisms was evaluated through in vitro fermentation. The results indicated that RX and XOS exhibited sugar utilization rates of 82.5 % and 100 % after purification, respectively, following 24 h of anaerobic fermentation with gut microbiota. Meanwhile, microbial concentration increased by 1.7-fold and 1.9-fold, with short-chain fatty acid production reaching 2.2 g/L and 3.2 g/L, respectively. The gut microbiota composition analysis revealed that RX enhanced species diversity, while XOS significantly enriched dominant taxa. RX promoted the growth of Actinobacteria and Bacteroidetes, whereas XOS favored Firmicutes and Actinobacteria and inhibited Proteobacteria. Functional prediction indicated that RX primarily enhanced cofactor and vitamin metabolism, energy metabolism, and cell growth pathways, while XOS activated pathways related to biodegradation, terpene and polyketide metabolism, and arginine and proline metabolism. These results provide a theoretical basis for the valorization of eucalyptus residues and prebiotic development.
{"title":"Prebiotic activity of xylo-oligosaccharides from eucalyptus forestry residues: Influence of polymerization degree on gut microbiota and metabolism","authors":"Xiaoxue Zhao , Yang Zhou , Caoxing Huang , Chenhuan Lai , Zhe Ling , Chunhui Liu , Qiang Yong","doi":"10.1016/j.fbp.2025.10.010","DOIUrl":"10.1016/j.fbp.2025.10.010","url":null,"abstract":"<div><div>The polymerization degree of xylo-oligosaccharides is crucial in determining their prebiotic activity. Herein, soluble xylan with a moderate degree of polymerization (RX) and xylo-oligosaccharides enriched in xylobiose and xylotriose (XOS) were prepared from eucalyptus forestry residues. Their influence on gut microbiota and potential metabolic mechanisms was evaluated through in vitro fermentation. The results indicated that RX and XOS exhibited sugar utilization rates of 82.5 % and 100 % after purification, respectively, following 24 h of anaerobic fermentation with gut microbiota. Meanwhile, microbial concentration increased by 1.7-fold and 1.9-fold, with short-chain fatty acid production reaching 2.2 g/L and 3.2 g/L, respectively. The gut microbiota composition analysis revealed that RX enhanced species diversity, while XOS significantly enriched dominant taxa. RX promoted the growth of <em>Actinobacteria</em> and Bacteroidetes, whereas XOS favored <em>Firmicutes</em> and <em>Actinobacteria</em> and inhibited <em>Proteobacteria</em>. Functional prediction indicated that RX primarily enhanced cofactor and vitamin metabolism, energy metabolism, and cell growth pathways, while XOS activated pathways related to biodegradation, terpene and polyketide metabolism, and arginine and proline metabolism. These results provide a theoretical basis for the valorization of eucalyptus residues and prebiotic development.</div></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":"154 ","pages":"Pages 500-508"},"PeriodicalIF":3.4,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-09DOI: 10.1016/j.fbp.2025.10.007
Zhili Shen, Shaohua Liang, Cong Sun, Pengcheng Meng, Ruinan Yang
The main factors influencing the crystallization process of crude phytosterols, such as the crystallization termination temperature, cooling rate, stirring speed, and number of recrystallization cycles, were systematically investigated in the present study. Six solvents were selected to extract phytosterols from corn oil deodorizer distillate, while the total phytosterol content and yield were used as evaluation indexes. The results indicated that the total phytosterol content in various solvents increased with higher crystallization termination temperatures, faster stirring rates, or a greater number of crystallization cycles. For the above three factors, taking ethyl acetate as an exemplar, the variations in total phytosterol content were from 70.24 % to 87.57 %, 84.13–92.81 %, and 96.43–99.91 %, respectively. However, under these conditions, the yield of total phytosterol decreased to varying extents. The total phytosterol content in various solvents decreased as the cooling rate increased, whereas the yield of total phytosterol showed varying degrees of increase. The morphological analysis of phytosterol crystals demonstrated that the crystal structure of phytosterol exhibits significant variation depending on the solvent employed, thereby influencing the separation and purification processes of phytosterol crystals. The Pearson correlation coefficient analysis revealed a strong correlation between the selected factors with the total phytosterol content and yield across various solvents. Furthermore, the heat map clustering results indicated that recrystallization using ethyl acetate had a significant effect compared to other solvents, achieving a total phytosterol content of 96.43 % and a total phytosterol yield of 94.25 %. This work holds considerable theoretical and technical significance for the industrialized and efficient production of high-purity phytosterol products. It also provides a feasible solution for the high-value utilization of by-products in vegetable oil processing.
{"title":"Effect of solvent recrystallization on the purification of crude phytosterols derived from corn oil deodorizer distillate","authors":"Zhili Shen, Shaohua Liang, Cong Sun, Pengcheng Meng, Ruinan Yang","doi":"10.1016/j.fbp.2025.10.007","DOIUrl":"10.1016/j.fbp.2025.10.007","url":null,"abstract":"<div><div>The main factors influencing the crystallization process of crude phytosterols, such as the crystallization termination temperature, cooling rate, stirring speed, and number of recrystallization cycles, were systematically investigated in the present study. Six solvents were selected to extract phytosterols from corn oil deodorizer distillate, while the total phytosterol content and yield were used as evaluation indexes. The results indicated that the total phytosterol content in various solvents increased with higher crystallization termination temperatures, faster stirring rates, or a greater number of crystallization cycles. For the above three factors, taking ethyl acetate as an exemplar, the variations in total phytosterol content were from 70.24 % to 87.57 %, 84.13–92.81 %, and 96.43–99.91 %, respectively. However, under these conditions, the yield of total phytosterol decreased to varying extents. The total phytosterol content in various solvents decreased as the cooling rate increased, whereas the yield of total phytosterol showed varying degrees of increase. The morphological analysis of phytosterol crystals demonstrated that the crystal structure of phytosterol exhibits significant variation depending on the solvent employed, thereby influencing the separation and purification processes of phytosterol crystals. The Pearson correlation coefficient analysis revealed a strong correlation between the selected factors with the total phytosterol content and yield across various solvents. Furthermore, the heat map clustering results indicated that recrystallization using ethyl acetate had a significant effect compared to other solvents, achieving a total phytosterol content of 96.43 % and a total phytosterol yield of 94.25 %. This work holds considerable theoretical and technical significance for the industrialized and efficient production of high-purity phytosterol products. It also provides a feasible solution for the high-value utilization of by-products in vegetable oil processing.</div></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":"154 ","pages":"Pages 379-391"},"PeriodicalIF":3.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145320425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-09DOI: 10.1016/j.fbp.2025.10.001
J. Del Rosario-Santiago , K.H. Estévez-Sánchez , H. Ruiz-Espinosa , O. Cortés-Zavaleta , J. Carrillo-Ahumada , C.E. Ochoa-Velasco , I.I. Ruiz-López
Analytical solutions to unsteady-state mass transfer (MT) equations are essential in many chemical and food engineering applications but exist only for a limited set of geometries. This study presents a simplified approach to obtain local and volume-averaged (VA) solutions for MT in elliptic cylinders (ECYLs). To this end, a dimensionless MT model for ECYLs was numerically solved for several aspect ratios and processing conditions. The resulting VA solutions were compared with the analytical solution for circular cylinders (CCYL) to derive a proportionality factor enabling time equivalence between these geometries. Spatial equivalence for local solutions was achieved by mapping ellipse coordinates to an equivalent radius within a unit circle. The proposed models were applied to analyze 3D MT during the dry salting of shrimp, estimating mass diffusivities () for water, total solids, and NaCl, as well as equilibrium dewatering and impregnation levels (). The proposed CCYL-based method closely matched the ECYL results, with differences in and under 1 % and 0.2 %, respectively. These differences were significantly smaller than the uncontrolled variability in parameter estimates, which ranged from 27 % to 45 % for and from 4 % to 14 % for . Estimated diffusivities were 3.0, 9.9, and 9.4 (×10−10) m2/s for water, total solids, NaCl, respectively. Overall, the proposed method demonstrates the feasibility of mapping multidimensional MT problems to simpler solids with 1D MT characteristics.
{"title":"A simplified solution for mass transfer in elliptic cylinders: Application to the dry salting of shrimp","authors":"J. Del Rosario-Santiago , K.H. Estévez-Sánchez , H. Ruiz-Espinosa , O. Cortés-Zavaleta , J. Carrillo-Ahumada , C.E. Ochoa-Velasco , I.I. Ruiz-López","doi":"10.1016/j.fbp.2025.10.001","DOIUrl":"10.1016/j.fbp.2025.10.001","url":null,"abstract":"<div><div>Analytical solutions to unsteady-state mass transfer (MT) equations are essential in many chemical and food engineering applications but exist only for a limited set of geometries. This study presents a simplified approach to obtain local and volume-averaged (VA) solutions for MT in elliptic cylinders (ECYLs). To this end, a dimensionless MT model for ECYLs was numerically solved for several aspect ratios and processing conditions. The resulting VA solutions were compared with the analytical solution for circular cylinders (CCYL) to derive a proportionality factor enabling time equivalence between these geometries. Spatial equivalence for local solutions was achieved by mapping ellipse coordinates to an equivalent radius within a unit circle. The proposed models were applied to analyze 3D MT during the dry salting of shrimp, estimating mass diffusivities (<span><math><mi>D</mi></math></span>) for water, total solids, and NaCl, as well as equilibrium dewatering and impregnation levels (<span><math><msub><mrow><mi>y</mi></mrow><mrow><mi>e</mi></mrow></msub></math></span>). The proposed CCYL-based method closely matched the ECYL results, with differences in <span><math><mi>D</mi></math></span> and <span><math><msub><mrow><mi>y</mi></mrow><mrow><mi>e</mi></mrow></msub></math></span> under 1 % and 0.2 %, respectively. These differences were significantly smaller than the uncontrolled variability in parameter estimates, which ranged from 27 % to 45 % for <span><math><mi>D</mi></math></span> and from 4 % to 14 % for <span><math><msub><mrow><mi>y</mi></mrow><mrow><mi>e</mi></mrow></msub></math></span>. Estimated diffusivities were 3.0, 9.9, and 9.4 (×10<sup>−10</sup>) m<sup>2</sup>/s for water, total solids, NaCl, respectively. Overall, the proposed method demonstrates the feasibility of mapping multidimensional MT problems to simpler solids with 1D MT characteristics.</div></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":"154 ","pages":"Pages 402-414"},"PeriodicalIF":3.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145320434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-08DOI: 10.1016/j.fbp.2025.10.006
Quynh Dao Nguyen , Kien Hao Tran , Ba Thanh Nguyen , Phuong Tuyen Nguyen , Minh Hai Nguyen , Quang Hieu Tran
This study details the development and evaluation of novel nanoliposomes for the co-delivery of adenosine and cordycepin from Cordyceps militaris extract, alongside curcumin. The nanoliposomes, prepared via solvent injection, exhibited a stable formulation (119.5 ± 2.7 nm; zeta potential −39.93 ± 0.25 mV) and high encapsulation efficiencies: adenosine (89.3 ± 1.6 %), cordycepin (90.6 ± 0.9 %), and curcumin (95.2 ± 2.1 %). In vitro digestion studies showed approximately 18 % cumulative release in simulated gastric fluid, followed by > 84 % release in simulated intestinal fluid. Kinetic modeling revealed zero-order release in the gastric phase and Korsmeyer-Peppas kinetics (non-Fickian diffusion) in the intestinal phase. The formulation also demonstrated improved in vitro antioxidant capacity (IC50 127.8 µg/mL), antibacterial activity against Staphylococcus aureus and Escherichia coli, and anticancer activity against MCF-7 cells (IC50 9.3 µg/mL). The enhanced stability, bioavailability, and synergistic biological activities of this nanoliposome highlight its potential as a functional food ingredient or nutraceutical for promoting health and combating cancer.
{"title":"Novel advanced nanoliposomes incorporating adenosine, cordycepin from riched-Cordyceps militaris extract, and curcumin: Formulation, stability, in vitro digestion, and enhanced biological activities","authors":"Quynh Dao Nguyen , Kien Hao Tran , Ba Thanh Nguyen , Phuong Tuyen Nguyen , Minh Hai Nguyen , Quang Hieu Tran","doi":"10.1016/j.fbp.2025.10.006","DOIUrl":"10.1016/j.fbp.2025.10.006","url":null,"abstract":"<div><div>This study details the development and evaluation of novel nanoliposomes for the co-delivery of adenosine and cordycepin from <em>Cordyceps militaris</em> extract, alongside curcumin. The nanoliposomes, prepared via solvent injection, exhibited a stable formulation (119.5 ± 2.7 nm; zeta potential −39.93 ± 0.25 mV) and high encapsulation efficiencies: adenosine (89.3 ± 1.6 %), cordycepin (90.6 ± 0.9 %), and curcumin (95.2 ± 2.1 %). <em>In vitro</em> digestion studies showed approximately 18 % cumulative release in simulated gastric fluid, followed by > 84 % release in simulated intestinal fluid. Kinetic modeling revealed zero-order release in the gastric phase and Korsmeyer-Peppas kinetics (non-Fickian diffusion) in the intestinal phase. The formulation also demonstrated improved <em>in vitro</em> antioxidant capacity (IC50 127.8 µg/mL), antibacterial activity against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>, and anticancer activity against MCF-7 cells (IC50 9.3 µg/mL). The enhanced stability, bioavailability, and synergistic biological activities of this nanoliposome highlight its potential as a functional food ingredient or nutraceutical for promoting health and combating cancer.</div></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":"154 ","pages":"Pages 334-349"},"PeriodicalIF":3.4,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-06DOI: 10.1016/j.fbp.2025.10.005
Dmitry D. Evtyugin, Dmitry V. Evtuguin
Samples with different contents of ellagic acid (EA) (55–94 %), isolated from sulphite spent liquor (SSL) of acid sulphite pulping of Eucalyptus wood, were examined for their antioxidant (AOX) activity towards the stable free 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and compared with several natural and synthetic antioxidants. Using a spectrophotometric approach with stoichiometric and kinetic parameters, AOX potentials were ordered as follows: EA > pyrogallol > caffeic acid ≈ Trolox > > butylated hydroxytoluene. Although the AOX activity in EA formulations was mainly attributed to EA, the contribution of concomitant lignin was not negligible. The importance of prior purification of EA formulations for enhanced AOX properties was highlighted. No significant synergistic or antagonistic effects of concomitants on the AOX activity of EA were detected. EA was suggested to be a prominent natural alternative to synthetic AOX for food applications and polymer matrices in food packaging, being a potentially safe solution for health and environment.
{"title":"A comprehensive DPPH study on the antioxidant activity of ellagic acid from Eucalyptus sulphite pulping liquor for food applications","authors":"Dmitry D. Evtyugin, Dmitry V. Evtuguin","doi":"10.1016/j.fbp.2025.10.005","DOIUrl":"10.1016/j.fbp.2025.10.005","url":null,"abstract":"<div><div>Samples with different contents of ellagic acid (EA) (55–94 %), isolated from sulphite spent liquor (SSL) of acid sulphite pulping of <em>Eucalyptus</em> wood, were examined for their antioxidant (AOX) activity towards the stable free 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and compared with several natural and synthetic antioxidants. Using a spectrophotometric approach with stoichiometric and kinetic parameters, AOX potentials were ordered as follows: EA > pyrogallol > caffeic acid ≈ Trolox > > butylated hydroxytoluene. Although the AOX activity in EA formulations was mainly attributed to EA, the contribution of concomitant lignin was not negligible. The importance of prior purification of EA formulations for enhanced AOX properties was highlighted. No significant synergistic or antagonistic effects of concomitants on the AOX activity of EA were detected. EA was suggested to be a prominent natural alternative to synthetic AOX for food applications and polymer matrices in food packaging, being a potentially safe solution for health and environment.</div></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":"154 ","pages":"Pages 350-358"},"PeriodicalIF":3.4,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The fish processing industry generates significant waste, including scales, bones, and skin, contributing to environmental challenges. Freshwater fish scales offer potential for extracting valuable biomaterials such as collagen, gelatin, and hydroxyapatite, which have diverse industrial applications. Recent research has focused on extracting type I collagen, gelatin, and hydroxyapatite from fish scales as suitable alternatives to animal-derived products, addressing health concerns and religious restrictions in many parts of the world. The review emphasizes established methods for extracting collagen, gelatin, and hydroxyapatite from freshwater fish scales, along with their functional properties and diverse industrial applications. This study addresses a gap in literature by exploring the economic potential of valorizing whole freshwater fish scales, while focusing on established techniques for extraction of key biomaterials such as collagen, gelatin, and hydroxyapatite and emphasizes their diverse applications in the biomedical, food, and packaging sectors. This review also addresses the safety regulations and future prospects for valorizing fish scales. Through comprehensive analysis and exploration, this research contributes to sustainable resource utilization in the fish processing industry.
{"title":"Extraction and industrial valorization of collagen, gelatin, and hydroxyapatite from freshwater fish scales: A review","authors":"Md. Amjad Patwary , Md. Yasin , Md. Shofiul Azam , Mohammad Afzal Hossain","doi":"10.1016/j.fbp.2025.10.004","DOIUrl":"10.1016/j.fbp.2025.10.004","url":null,"abstract":"<div><div>The fish processing industry generates significant waste, including scales, bones, and skin, contributing to environmental challenges. Freshwater fish scales offer potential for extracting valuable biomaterials such as collagen, gelatin, and hydroxyapatite, which have diverse industrial applications. Recent research has focused on extracting type I collagen, gelatin, and hydroxyapatite from fish scales as suitable alternatives to animal-derived products, addressing health concerns and religious restrictions in many parts of the world. The review emphasizes established methods for extracting collagen, gelatin, and hydroxyapatite from freshwater fish scales, along with their functional properties and diverse industrial applications. This study addresses a gap in literature by exploring the economic potential of valorizing whole freshwater fish scales, while focusing on established techniques for extraction of key biomaterials such as collagen, gelatin, and hydroxyapatite and emphasizes their diverse applications in the biomedical, food, and packaging sectors. This review also addresses the safety regulations and future prospects for valorizing fish scales. Through comprehensive analysis and exploration, this research contributes to sustainable resource utilization in the fish processing industry.</div></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":"154 ","pages":"Pages 319-333"},"PeriodicalIF":3.4,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}