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Biodegradable cryogels based on pea starch as carriers of bioactive compounds from pecan (Carya illinoinensis) co-product: Synthesis, characterization, and prospecting for technological use 以豌豆淀粉为载体的山核桃副产物生物活性化合物的可生物降解冷冻材料:合成、表征及技术应用前景
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-10-15 DOI: 10.1016/j.fbp.2025.10.014
Eduarda Caetano Peixoto , Elder Pacheco da Cruz , Tatiane Jéssica Siebeneichler , Laura Martins Fonseca , Julia Eisenhardt de Mello , Roselia Maria Spanevello , Alvaro Renato Guerra Dias , Elessandra da Rosa Zavareze , Cesar Valmor Rombaldi
Pecan nut shells are typically discarded as waste. However, their valorization holds significant potential due to the presence of phenolic compounds and antioxidant properties. This study sought to enhance the value of this byproduct by developing cryogels derived from pea starch, enriched with pecan nut shell extract (PNSE). Develop a fully biodegradable material suitable for food packaging that is capable of incorporating bioactive compounds. The cryogels were prepared by gelatinizing the starch, followed by physical crosslinking through five cycles of freezing and thawing. PNSE was incorporated into the cryogels at 10 % and 20 % (w/w). PNSE was evaluated for its individual phenolic compounds and cytotoxicity. The cryogels were assessed for their morphology, density, porosity, water absorption, hardness, antioxidant activity, reuse potential, and biodegradability. PNSE contained phenolic compounds, with tannins and procyanidins identified as the major groups. These compounds are primarily responsible for the bioactivity of PNSE. The cryogels exhibited a macroporous and homogeneous morphology. All cryogels had a low density (0.058–0.078 g/cm³), high porosity (88–91 %), and high water absorption, reaching up to 1513 %. The incorporation of PNSE increased the hardness of the cryogels, thereby enhancing their structural integrity. Cryogels demonstrated reusability over 15 cycles, with 600 % absorption potential. All cryogels were biodegradable in soil within 15 days. These cryogels represent effective matrices with potential applications as water absorbers for fresh food products that release exudates, as well as carriers of antioxidant and bioactive compounds.
山核桃壳通常被当作废物丢弃。然而,由于酚类化合物和抗氧化性能的存在,它们的增值具有显著的潜力。本研究试图通过开发富含核桃壳提取物(PNSE)的豌豆淀粉衍生的冷冻产品来提高这一副产品的价值。开发一种完全可生物降解的材料,适合于食品包装,能够纳入生物活性化合物。通过将淀粉糊化,然后经过5次冷冻和解冻的物理交联,制备出冷冻材料。PNSE分别以10 %和20 % (w/w)加入到冷冻液中。评估了PNSE的单个酚类化合物和细胞毒性。研究人员评估了这些冷冻液的形态、密度、孔隙率、吸水性、硬度、抗氧化活性、再利用潜力和生物降解性。PNSE含有酚类化合物,以单宁和原花青素为主要基团。这些化合物是PNSE生物活性的主要原因。所制备的冷库具有均匀的大孔结构。所有冷冻液均具有低密度(0.058 ~ 0.078 g/cm³)、高孔隙率(88 ~ 91 %)和高吸水性(高达1513 %)的特点。PNSE的加入提高了低温材料的硬度,从而增强了其结构完整性。制冷机可重复使用超过15次循环,具有600 %的吸收潜力。所有冷冻剂在15天内均可在土壤中降解。这些冷藏箱代表了有效的基质,具有潜在的应用价值,可以作为新鲜食品中释放渗出物的吸水性材料,以及抗氧化剂和生物活性化合物的载体。
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引用次数: 0
Proof-of-concept: Ultrafiltration as a pre-treatment for the anion exchange chromatography of RuBisCO from alfalfa 概念验证:超滤作为紫花苜蓿RuBisCO阴离子交换色谱的预处理
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-10-13 DOI: 10.1016/j.fbp.2025.10.011
Hartono Tanambell , Marianne Danielsen , Anders Hauer Møller , Trine Kastrup Dalsgaard
Alfalfa is gaining interest as an alternative protein source. The European Food Safety Authority (EFSA) approval of alfalfa protein concentrate as novel food states presence of metabolites as a barrier for higher amount of consumption. A promising method for protein isolation is anion exchange chromatography, which is scalable through expanded bed chromatography. In this study, ultrafiltration-buffer exchange (UBE) and ultrafiltration-diafiltration was applied as an upstream and downstream processing to anion exchange chromatography, respectively. The performance of these techniques was compared to that of lab scale dilution and centrifugal spin filters. The result showed that UBE shortened the chromatography duration by 50 % and resulted in a cleaner column. However, downstream desalination through ultrafiltration-diafiltration resulted in concentrate with lower protein content (60.41 ± 0.84 %), compared to that obtained from centrifugal spin filters (90.49 ± 1.34 %), suggesting the need to carry out extra diafiltration steps. Untargeted metabolomics revealed that high absorption at 214 nm in the anion exchange flowthrough fraction could be attributed to di- and tripeptides also present in the ultrafiltration permeates. Polyphenols present in ultrafiltration permeate were absent from both flowthrough and 225 mM fraction, suggesting that they might bind strongly to the column. Various carboxylic acids were present in the ultrafiltration permeate, chromatography flowthrough, and 225 mM NaCl fraction. Contaminants eluting at 225 mM NaCl with high absorbance at 214 nm were not successfully identified although their occurrence was lower in UBE samples. These results suggest that UBE is promising as pre-treatment for purification of RuBisCO from alfalfa using anion exchange chromatography.
苜蓿作为一种替代蛋白质来源正引起人们的兴趣。欧洲食品安全局(EFSA)批准苜蓿蛋白浓缩物作为新型食品,指出其代谢物的存在是大量食用的障碍。阴离子交换色谱法是一种很有前途的蛋白质分离方法,它可以通过扩展床色谱法进行扩展。在本研究中,超滤-缓冲交换(UBE)和超滤-滤滤分别作为阴离子交换色谱的上游和下游处理。将这些技术的性能与实验室规模稀释和离心自旋过滤器的性能进行了比较。结果表明,UBE使色谱时间缩短了50% %,色谱柱更干净。然而,与离心自旋过滤器获得的浓缩物(90.49 ± 1.34 %)相比,下游通过超滤-滤除得到的浓缩物蛋白质含量(60.41 ± 0.84 %)较低,这表明需要进行额外的滤除步骤。非靶向代谢组学显示,在214 nm处阴离子交换流过部分的高吸收可能归因于超滤渗透物中也存在的二肽和三肽。超滤渗透液中存在的多酚在流过和225 mM馏分中均不存在,表明它们可能与柱结合强烈。超滤渗透液、色谱流和225 mM NaCl馏分中存在不同的羧酸。在225 mM NaCl洗脱的214 nm高吸光度的污染物虽然在UBE样品中的发生率较低,但未能成功鉴定。这些结果表明,UBE有望作为阴离子交换色谱法纯化紫花苜蓿中RuBisCO的前处理。
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引用次数: 0
A new strategy with forming the aggregation unit to improve chlorophyll water solubility based on its natural inherent structure carriers 基于天然固有结构载体形成聚集单元提高叶绿素水溶性的新策略
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-10-10 DOI: 10.1016/j.fbp.2025.10.008
Zhaotian Yang , Jinghao Zhang , Suxia Shen , Ning Xu , Zixuan Han , Lin Zeng , Yan Zhang
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Chlorophyll (Chl), a natural pigment with multifunctional bioactive properties, faces limited industrial application due to its poor water solubility. In this study, we proposed a novel strategy utilizing Chl-rich spinach extracts (CRSE)—natural Chl carriers with inherent structural stability—to generate water-soluble Chl particles. CRSE contained high protein (47.57 %) and lipid (8.2 %) contents, enabling its use as a structural foundation. Sucrose fatty acid ester (SE) and maltodextrin (MD)-whey protein isolate (WPI) matrix were chosen to stabilize emulsions and generate CRSE-MD-WPI aggregation unit particles. The optimized formulation (1.5 % SE; 14 % wall materials, WPI:MD = 5:5) produced particles with enhanced encapsulation efficiency (81.75 %), solubility (86.83 %), and reduced moisture content (3.83 %). Structural analyses revealed that WPI established hydrophobic interactions with CRSE, hydrogen bonding with MD, and alterations both CRSE microenvironment polarity and MD crystallinity, thereby improving solubility and stability. Importantly, this approach reduced wall material usage by 50 % compared to conventional encapsulation. Overall, this work presents a scalable and eco-friendly protocol to enhance Chl solubility, providing a promising pathway for functional pigment applications in the food industry.

Graphical abstract
叶绿素(Chl)是一种具有多功能生物活性的天然色素,由于其水溶性较差,工业应用受到限制。在这项研究中,我们提出了一种新的策略,利用富含Chl的菠菜提取物(CRSE) -具有固有结构稳定性的天然Chl载体-生成水溶性Chl颗粒。CRSE含有较高的蛋白质(47.57 %)和脂质(8.2 %)含量,使其成为结构基础。选择蔗糖脂肪酸酯(SE)和麦芽糖糊精(MD)-乳清分离蛋白(WPI)基质稳定乳状液,生成CRSE-MD-WPI聚集单元颗粒。优化后的配方(1.5 % SE; 14 %壁材,WPI:MD = 5:5)制备的颗粒包封效率提高(81.75 %),溶解度提高(86.83 %),含水率降低(3.83 %)。结构分析表明,WPI与CRSE建立疏水相互作用,与MD形成氢键,改变了CRSE微环境极性和MD结晶度,从而提高了溶解性和稳定性。重要的是,与传统封装相比,这种方法减少了50% %的壁材使用量。总的来说,这项工作提出了一种可扩展和环保的方案来提高Chl的溶解度,为功能性色素在食品工业中的应用提供了一条有前途的途径。图形抽象
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引用次数: 0
Ultrasound pretreatment improves 3D printing and gelling properties of low-salt sliver carp (Hypophthalmichthys molitrix) surimi 超声预处理改善了低盐鲢鱼鱼糜的3D打印和胶凝性能
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-10-10 DOI: 10.1016/j.fbp.2025.10.009
Yaxing Yang , Liyun Wu , Qingqing Zeng , Feifan Cao , Hu Shi , Juming Tang , Fanbin Kong , Zhe Wang , Yang Jiao
3D printing provides delicate and unique structure for low-salt surimi product development, but may require pretreatment methods for improving its stability and gel strength. This study investigated the effects of ultrasound pretreatments (400 W, 40 kHz, treatment time of 0–30 min) on the 3D printing and gelling properties of low-salt surimi. The results revealed that ultrasound pretreatment improved the surface smoothness of printed samples and decreased the filament breakage. The gel strength, water holding capacity and salt-soluble protein solubility of surimi gel initially increased with longer ultrasonic treatment times and then decreased, which indicated that appropriate ultrasound pretreatment promoted the interaction between protein molecules and water. As a result, more proteins participated in the formation of the gel network due to ultrasound application, which significantly enhanced the hardness and gel strength of the low-salt surimi gel. The change in total sulfhydryl content showed a contrary trend than the protein solubility, which might be attributed to more pronounced protein unfolding induced by ultrasound and subsequent exposure of -SH groups, leading to formation of more disulfide bonds. The low field NMR and MRI showed that the initial relaxation times were shortened and hydrogen proton density increased, indicating a tighter binding between protein and water and reduced water fluidity. Scanning electron microscope observations showed the surimi gel treated with ultrasound for 15 min formed a uniform and dense gel network. In conclusion, appropriate ultrasound pretreatment significantly improves the 3D printing and gelling properties of low-salt sliver carp surimi, which provides a promising method in low-salt surimi-based food development in 3D printing.
3D打印为低盐鱼糜产品的开发提供了精致独特的结构,但可能需要预处理方法来提高其稳定性和凝胶强度。本研究考察了超声预处理(400 W, 40 kHz,处理时间0-30 min)对低盐鱼糜3D打印及胶凝性能的影响。结果表明,超声预处理提高了打印样品的表面光洁度,减少了细丝断裂。随着超声处理时间的延长,鱼糜凝胶的凝胶强度、持水量和盐溶蛋白溶解度先升高后降低,说明适当的超声预处理促进了蛋白质分子与水的相互作用。超声的应用使得更多的蛋白质参与凝胶网络的形成,显著提高了低盐鱼糜凝胶的硬度和凝胶强度。总巯基含量的变化与蛋白质溶解度的变化趋势相反,这可能是由于超声和随后的-SH基团暴露导致蛋白质展开更明显,导致形成更多的二硫键。低场NMR和MRI显示,初始弛豫时间缩短,氢质子密度增加,表明蛋白质与水的结合更紧密,水的流动性降低。扫描电镜观察显示,超声作用15 min后鱼糜凝胶形成均匀致密的凝胶网络。综上所述,适当的超声预处理可以显著提高低盐鲢鱼鱼糜的3D打印和胶凝性能,为低盐鱼糜食品的3D打印开发提供了一种很有前景的方法。
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引用次数: 0
Prebiotic activity of xylo-oligosaccharides from eucalyptus forestry residues: Influence of polymerization degree on gut microbiota and metabolism 桉树残基低聚木糖的益生元活性:聚合程度对肠道菌群和代谢的影响
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-10-10 DOI: 10.1016/j.fbp.2025.10.010
Xiaoxue Zhao , Yang Zhou , Caoxing Huang , Chenhuan Lai , Zhe Ling , Chunhui Liu , Qiang Yong
The polymerization degree of xylo-oligosaccharides is crucial in determining their prebiotic activity. Herein, soluble xylan with a moderate degree of polymerization (RX) and xylo-oligosaccharides enriched in xylobiose and xylotriose (XOS) were prepared from eucalyptus forestry residues. Their influence on gut microbiota and potential metabolic mechanisms was evaluated through in vitro fermentation. The results indicated that RX and XOS exhibited sugar utilization rates of 82.5 % and 100 % after purification, respectively, following 24 h of anaerobic fermentation with gut microbiota. Meanwhile, microbial concentration increased by 1.7-fold and 1.9-fold, with short-chain fatty acid production reaching 2.2 g/L and 3.2 g/L, respectively. The gut microbiota composition analysis revealed that RX enhanced species diversity, while XOS significantly enriched dominant taxa. RX promoted the growth of Actinobacteria and Bacteroidetes, whereas XOS favored Firmicutes and Actinobacteria and inhibited Proteobacteria. Functional prediction indicated that RX primarily enhanced cofactor and vitamin metabolism, energy metabolism, and cell growth pathways, while XOS activated pathways related to biodegradation, terpene and polyketide metabolism, and arginine and proline metabolism. These results provide a theoretical basis for the valorization of eucalyptus residues and prebiotic development.
低聚木糖的聚合度是决定其益生元活性的关键。本文以桉树残基为原料,制备了中等聚合度的可溶性木聚糖(RX)和富含木糖二糖和木糖三糖的低聚木糖(XOS)。通过体外发酵,评估其对肠道菌群的影响和潜在的代谢机制。结果表明,纯化后的RX和XOS经肠道菌群厌氧发酵24 h,糖利用率分别为82.5 %和100 %。微生物浓度提高了1.7倍和1.9倍,短链脂肪酸产量分别达到2.2 g/L和3.2 g/L。肠道菌群组成分析显示,RX提高了物种多样性,而XOS显著增加了优势类群。RX促进放线菌门和拟杆菌门的生长,XOS有利于厚壁菌门和放线菌门的生长,抑制变形菌门的生长。功能预测表明,RX主要增强了辅助因子和维生素代谢、能量代谢和细胞生长途径,而XOS激活了生物降解、萜烯和聚酮代谢、精氨酸和脯氨酸代谢等相关途径。这些结果为桉树残留物的增值和益生元的开发提供了理论依据。
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引用次数: 0
Effect of solvent recrystallization on the purification of crude phytosterols derived from corn oil deodorizer distillate 溶剂重结晶对玉米油脱臭馏出物纯化粗植物甾醇的影响
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-10-09 DOI: 10.1016/j.fbp.2025.10.007
Zhili Shen, Shaohua Liang, Cong Sun, Pengcheng Meng, Ruinan Yang
The main factors influencing the crystallization process of crude phytosterols, such as the crystallization termination temperature, cooling rate, stirring speed, and number of recrystallization cycles, were systematically investigated in the present study. Six solvents were selected to extract phytosterols from corn oil deodorizer distillate, while the total phytosterol content and yield were used as evaluation indexes. The results indicated that the total phytosterol content in various solvents increased with higher crystallization termination temperatures, faster stirring rates, or a greater number of crystallization cycles. For the above three factors, taking ethyl acetate as an exemplar, the variations in total phytosterol content were from 70.24 % to 87.57 %, 84.13–92.81 %, and 96.43–99.91 %, respectively. However, under these conditions, the yield of total phytosterol decreased to varying extents. The total phytosterol content in various solvents decreased as the cooling rate increased, whereas the yield of total phytosterol showed varying degrees of increase. The morphological analysis of phytosterol crystals demonstrated that the crystal structure of phytosterol exhibits significant variation depending on the solvent employed, thereby influencing the separation and purification processes of phytosterol crystals. The Pearson correlation coefficient analysis revealed a strong correlation between the selected factors with the total phytosterol content and yield across various solvents. Furthermore, the heat map clustering results indicated that recrystallization using ethyl acetate had a significant effect compared to other solvents, achieving a total phytosterol content of 96.43 % and a total phytosterol yield of 94.25 %. This work holds considerable theoretical and technical significance for the industrialized and efficient production of high-purity phytosterol products. It also provides a feasible solution for the high-value utilization of by-products in vegetable oil processing.
系统研究了影响植物甾醇粗结晶过程的主要因素,如结晶终止温度、冷却速率、搅拌速度、再结晶循环次数等。选择6种溶剂从玉米油脱臭馏出物中提取植物甾醇,以总甾醇含量和得率为评价指标。结果表明,随着结晶终止温度的升高、搅拌速率的加快和结晶循环次数的增加,不同溶剂中植物甾醇的含量均有所增加。以乙酸乙酯为例,3个因素对总甾醇含量的影响范围分别为70.24 % ~ 87.57 %、84.13 ~ 92.81 %和96.43 ~ 99.91 %。然而,在这些条件下,总甾醇的产率都有不同程度的下降。随着冷却速率的增加,各种溶剂中的总甾醇含量降低,而总甾醇得率则有不同程度的提高。对植物甾醇晶体的形态分析表明,不同溶剂对植物甾醇晶体结构的影响很大,从而影响了植物甾醇晶体的分离纯化过程。Pearson相关系数分析表明,所选因素与不同溶剂的总甾醇含量和得率之间存在较强的相关性。此外,热图聚类结果表明,与其他溶剂相比,乙酸乙酯重结晶效果显著,总甾醇含量为96.43 %,总甾醇收率为94.25 %。本研究对工业化高效生产高纯度植物甾醇产品具有重要的理论和技术意义。为植物油加工副产物的高价值利用提供了可行的解决方案。
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引用次数: 0
A simplified solution for mass transfer in elliptic cylinders: Application to the dry salting of shrimp 椭圆圆柱体传质的简化解:在虾干腌渍中的应用
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-10-09 DOI: 10.1016/j.fbp.2025.10.001
J. Del Rosario-Santiago , K.H. Estévez-Sánchez , H. Ruiz-Espinosa , O. Cortés-Zavaleta , J. Carrillo-Ahumada , C.E. Ochoa-Velasco , I.I. Ruiz-López
Analytical solutions to unsteady-state mass transfer (MT) equations are essential in many chemical and food engineering applications but exist only for a limited set of geometries. This study presents a simplified approach to obtain local and volume-averaged (VA) solutions for MT in elliptic cylinders (ECYLs). To this end, a dimensionless MT model for ECYLs was numerically solved for several aspect ratios and processing conditions. The resulting VA solutions were compared with the analytical solution for circular cylinders (CCYL) to derive a proportionality factor enabling time equivalence between these geometries. Spatial equivalence for local solutions was achieved by mapping ellipse coordinates to an equivalent radius within a unit circle. The proposed models were applied to analyze 3D MT during the dry salting of shrimp, estimating mass diffusivities (D) for water, total solids, and NaCl, as well as equilibrium dewatering and impregnation levels (ye). The proposed CCYL-based method closely matched the ECYL results, with differences in D and ye under 1 % and 0.2 %, respectively. These differences were significantly smaller than the uncontrolled variability in parameter estimates, which ranged from 27 % to 45 % for D and from 4 % to 14 % for ye. Estimated diffusivities were 3.0, 9.9, and 9.4 (×10−10) m2/s for water, total solids, NaCl, respectively. Overall, the proposed method demonstrates the feasibility of mapping multidimensional MT problems to simpler solids with 1D MT characteristics.
非稳态传质(MT)方程的解析解在许多化学和食品工程应用中是必不可少的,但只适用于有限的几何形状。本研究提出了一种简化的方法来获得椭圆圆柱体中MT的局部解和体积平均(VA)解。为此,对不同宽高比和加工条件下的ECYLs无量纲MT模型进行了数值求解。将所得的VA解与圆柱(CCYL)的解析解进行比较,以得出使这些几何形状之间的时间等效的比例因子。通过将椭圆坐标映射到单位圆内的等效半径,实现了局部解的空间等价。该模型用于分析虾干盐过程中的三维MT,估计水、总固体和NaCl的质量扩散系数(D)以及平衡脱水和浸渍水平(ye)。所提出的基于ccyl的方法与ECYL结果非常吻合,D和ye的差异分别在1%和0.2%以下。这些差异明显小于参数估计的未控制变异性,D的范围为27%至45%,ye的范围为4%至14%。水、总固体和NaCl的估计扩散系数分别为3.0、9.9和9.4 (×10−10)m2/s。总体而言,该方法证明了将多维MT问题映射到具有一维MT特征的更简单实体的可行性。
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引用次数: 0
Novel advanced nanoliposomes incorporating adenosine, cordycepin from riched-Cordyceps militaris extract, and curcumin: Formulation, stability, in vitro digestion, and enhanced biological activities 含有腺苷、蛹虫草提取物虫草素和姜黄素的新型先进纳米脂质体:配方、稳定性、体外消化和增强的生物活性
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-10-08 DOI: 10.1016/j.fbp.2025.10.006
Quynh Dao Nguyen , Kien Hao Tran , Ba Thanh Nguyen , Phuong Tuyen Nguyen , Minh Hai Nguyen , Quang Hieu Tran
This study details the development and evaluation of novel nanoliposomes for the co-delivery of adenosine and cordycepin from Cordyceps militaris extract, alongside curcumin. The nanoliposomes, prepared via solvent injection, exhibited a stable formulation (119.5 ± 2.7 nm; zeta potential −39.93 ± 0.25 mV) and high encapsulation efficiencies: adenosine (89.3 ± 1.6 %), cordycepin (90.6 ± 0.9 %), and curcumin (95.2 ± 2.1 %). In vitro digestion studies showed approximately 18 % cumulative release in simulated gastric fluid, followed by > 84 % release in simulated intestinal fluid. Kinetic modeling revealed zero-order release in the gastric phase and Korsmeyer-Peppas kinetics (non-Fickian diffusion) in the intestinal phase. The formulation also demonstrated improved in vitro antioxidant capacity (IC50 127.8 µg/mL), antibacterial activity against Staphylococcus aureus and Escherichia coli, and anticancer activity against MCF-7 cells (IC50 9.3 µg/mL). The enhanced stability, bioavailability, and synergistic biological activities of this nanoliposome highlight its potential as a functional food ingredient or nutraceutical for promoting health and combating cancer.
本研究详细介绍了新型纳米脂质体的开发和评价,用于从蛹虫草提取物中共同递送腺苷和虫草素,以及姜黄素。nanoliposomes,准备通过溶剂注入,表现出一个稳定的配方( 119.5±2.7  nm;电动电势 −39.93±0.25  mV)和高封装效率:腺苷(89.3 ±1.6  %),虫草素(90.6 ±0.9  %),和姜黄素(95.2 ±2.1  %)。体外消化研究显示,在模拟胃液中累积释放量约为18% %,随后在模拟肠液中释放量为 84% %。动力学模型显示胃相为零级释放,肠相为Korsmeyer-Peppas动力学(非fickian扩散)。该制剂还显示出提高的体外抗氧化能力(IC50 127.8 µg/mL),对金黄色葡萄球菌和大肠杆菌的抗菌活性,以及对MCF-7细胞的抗癌活性(IC50 9.3 µg/mL)。这种纳米脂质体的稳定性、生物利用度和协同生物活性增强了其作为功能性食品成分或营养保健品促进健康和抗癌的潜力。
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引用次数: 0
A comprehensive DPPH study on the antioxidant activity of ellagic acid from Eucalyptus sulphite pulping liquor for food applications 食品用亚桉浆液鞣花酸抗氧化活性的DPPH综合研究
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-10-06 DOI: 10.1016/j.fbp.2025.10.005
Dmitry D. Evtyugin, Dmitry V. Evtuguin
Samples with different contents of ellagic acid (EA) (55–94 %), isolated from sulphite spent liquor (SSL) of acid sulphite pulping of Eucalyptus wood, were examined for their antioxidant (AOX) activity towards the stable free 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and compared with several natural and synthetic antioxidants. Using a spectrophotometric approach with stoichiometric and kinetic parameters, AOX potentials were ordered as follows: EA > pyrogallol > caffeic acid ≈ Trolox > > butylated hydroxytoluene. Although the AOX activity in EA formulations was mainly attributed to EA, the contribution of concomitant lignin was not negligible. The importance of prior purification of EA formulations for enhanced AOX properties was highlighted. No significant synergistic or antagonistic effects of concomitants on the AOX activity of EA were detected. EA was suggested to be a prominent natural alternative to synthetic AOX for food applications and polymer matrices in food packaging, being a potentially safe solution for health and environment.
从桉木亚硫酸盐制浆亚硫酸盐废液(SSL)中分离得到不同含量的鞣花酸(EA)(55 ~ 94 %),测定了其对稳定游离的2,2-二苯基-1-吡啶肼(DPPH)自由基的抗氧化活性,并与几种天然抗氧化剂和合成抗氧化剂进行了比较。采用具有化学计量学和动力学参数的分光光度法,AOX电位依次为:EA >; 邻苯三酚>; 咖啡酸≈ Trolox >; >;丁基羟基甲苯。虽然EA制剂的AOX活性主要归因于EA,但伴随木质素的贡献不可忽略。强调了事先纯化EA制剂对增强AOX性能的重要性。伴随物对EA的AOX活性没有明显的协同或拮抗作用。EA被认为是食品应用和食品包装聚合物基质中合成AOX的重要天然替代品,是一种潜在的健康和环境安全解决方案。
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引用次数: 0
Extraction and industrial valorization of collagen, gelatin, and hydroxyapatite from freshwater fish scales: A review 从淡水鱼鳞中提取胶原蛋白、明胶和羟基磷灰石及其工业价值:综述
IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2025-10-05 DOI: 10.1016/j.fbp.2025.10.004
Md. Amjad Patwary , Md. Yasin , Md. Shofiul Azam , Mohammad Afzal Hossain
The fish processing industry generates significant waste, including scales, bones, and skin, contributing to environmental challenges. Freshwater fish scales offer potential for extracting valuable biomaterials such as collagen, gelatin, and hydroxyapatite, which have diverse industrial applications. Recent research has focused on extracting type I collagen, gelatin, and hydroxyapatite from fish scales as suitable alternatives to animal-derived products, addressing health concerns and religious restrictions in many parts of the world. The review emphasizes established methods for extracting collagen, gelatin, and hydroxyapatite from freshwater fish scales, along with their functional properties and diverse industrial applications. This study addresses a gap in literature by exploring the economic potential of valorizing whole freshwater fish scales, while focusing on established techniques for extraction of key biomaterials such as collagen, gelatin, and hydroxyapatite and emphasizes their diverse applications in the biomedical, food, and packaging sectors. This review also addresses the safety regulations and future prospects for valorizing fish scales. Through comprehensive analysis and exploration, this research contributes to sustainable resource utilization in the fish processing industry.
鱼类加工业产生大量废物,包括鱼鳞、鱼骨和鱼皮,对环境构成挑战。淡水鱼鳞提供了提取有价值的生物材料的潜力,如胶原蛋白、明胶和羟基磷灰石,这些材料具有多种工业应用。最近的研究集中在从鱼鳞中提取I型胶原蛋白、明胶和羟基磷灰石,作为动物源性产品的合适替代品,以解决世界上许多地方的健康问题和宗教限制。综述了从淡水鱼鳞中提取胶原蛋白、明胶和羟基磷灰石的方法,以及它们的功能特性和多种工业应用。本研究通过探索整个淡水鱼鳞片的经济潜力来填补文献空白,同时重点关注胶原蛋白、明胶和羟基磷灰石等关键生物材料的提取技术,并强调其在生物医学、食品和包装领域的多种应用。本文还讨论了鱼鳞加工的安全法规和未来前景。通过综合分析和探索,本研究有助于鱼类加工业资源的可持续利用。
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引用次数: 0
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Food and Bioproducts Processing
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